Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010915.01.T01 | XP_002946472.1 | 98.63 | 73 | 1 | 0 | 1 | 73 | 1 | 73 | 3.81E-45 | 149 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010915.01.T01 | Q9FGZ9 | 93.151 | 73 | 5 | 0 | 1 | 73 | 1 | 73 | 1.33E-44 | 140 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010915.01.T01 | A0A835BB35 | 94.521 | 73 | 4 | 0 | 1 | 73 | 889 | 961 | 1.61e-41 | 153 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0380016133.01 | MsG0280010915.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010915.01.T01 | MTR_3g084140 | 95.890 | 73 | 3 | 0 | 1 | 73 | 1 | 73 | 5.79e-47 | 144 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010915.01.T01 | AT3G45180 | 93.151 | 73 | 5 | 0 | 1 | 73 | 1 | 73 | 4.28e-46 | 141 |
| MsG0280010915.01.T01 | AT3G45180 | 93.151 | 73 | 5 | 0 | 1 | 73 | 1 | 73 | 4.28e-46 | 141 |
| MsG0280010915.01.T01 | AT5G42300 | 93.151 | 73 | 5 | 0 | 1 | 73 | 1 | 73 | 1.36e-45 | 140 |
Find 12 sgRNAs with CRISPR-Local
Find 13 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACAAAATTCGGATTCAGAAA+TGG | 0.431017 | 2:+76843980 | MsG0280010915.01.T01:CDS |
| GTACAAGACCTGACAAAATT+CGG | 0.445316 | 2:+76843968 | MsG0280010915.01.T01:CDS |
| GAACGATCGGTTAGGGAAGA+AGG | 0.472486 | 2:+76843879 | MsG0280010915.01.T01:CDS |
| AAACTCGTTGCTGCACAAAC+AGG | 0.476026 | 2:+76843946 | MsG0280010915.01.T01:CDS |
| ATTAGTTATAGTAGAGTTCG+AGG | 0.477137 | 2:-76844062 | None:intergenic |
| TCGATCGAAGAAGATGATAG+AGG | 0.558716 | 2:+76843849 | None:intergenic |
| TACGAGATTCATGATGGCAT+GGG | 0.579214 | 2:+76844039 | MsG0280010915.01.T01:CDS |
| TTACGAGATTCATGATGGCA+TGG | 0.596991 | 2:+76844038 | MsG0280010915.01.T01:CDS |
| TCTGATTACGAGATTCATGA+TGG | 0.607663 | 2:+76844033 | MsG0280010915.01.T01:CDS |
| GAGTTTCTTCAAGTCACCGA+TGG | 0.637090 | 2:-76843929 | None:intergenic |
| ATCGAAGAAGATGATAGAGG+TGG | 0.670468 | 2:+76843852 | None:intergenic |
| TGCAACGAAGATGATACCAT+CGG | 0.675479 | 2:+76843913 | MsG0280010915.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACAAAATTCGGATTCAGAAA+TGG | + | Chr2:76843980-76843999 | MsG0280010915.01.T01:CDS | 30.0% | |
| GTACAAGACCTGACAAAATT+CGG | + | Chr2:76843968-76843987 | MsG0280010915.01.T01:CDS | 35.0% | |
| TCTGATTACGAGATTCATGA+TGG | + | Chr2:76844033-76844052 | MsG0280010915.01.T01:CDS | 35.0% | |
| ! | TTCTGAATCCGAATTTTGTC+AGG | - | Chr2:76843979-76843998 | None:intergenic | 35.0% |
| TACGAGATTCATGATGGCAT+GGG | + | Chr2:76844039-76844058 | MsG0280010915.01.T01:CDS | 40.0% | |
| TGCAACGAAGATGATACCAT+CGG | + | Chr2:76843913-76843932 | MsG0280010915.01.T01:CDS | 40.0% | |
| TTACGAGATTCATGATGGCA+TGG | + | Chr2:76844038-76844057 | MsG0280010915.01.T01:CDS | 40.0% | |
| !!! | TAGAGGTGGTTTTGAACGAT+CGG | + | Chr2:76843866-76843885 | MsG0280010915.01.T01:CDS | 40.0% |
| !!! | TGGTTTTGAACGATCGGTTA+GGG | + | Chr2:76843872-76843891 | MsG0280010915.01.T01:CDS | 40.0% |
| AAACTCGTTGCTGCACAAAC+AGG | + | Chr2:76843946-76843965 | MsG0280010915.01.T01:CDS | 45.0% | |
| GAGTTTCTTCAAGTCACCGA+TGG | - | Chr2:76843932-76843951 | None:intergenic | 45.0% | |
| !!! | GTGGTTTTGAACGATCGGTT+AGG | + | Chr2:76843871-76843890 | MsG0280010915.01.T01:CDS | 45.0% |
| GAACGATCGGTTAGGGAAGA+AGG | + | Chr2:76843879-76843898 | MsG0280010915.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 76843862 | 76844083 | 76843862 | ID=MsG0280010915.01;Name=MsG0280010915.01 |
| Chr2 | mRNA | 76843862 | 76844083 | 76843862 | ID=MsG0280010915.01.T01;Parent=MsG0280010915.01;Name=MsG0280010915.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|73 |
| Chr2 | exon | 76843862 | 76844083 | 76843862 | ID=MsG0280010915.01.T01:exon:27901;Parent=MsG0280010915.01.T01 |
| Chr2 | CDS | 76843862 | 76844083 | 76843862 | ID=MsG0280010915.01.T01:cds;Parent=MsG0280010915.01.T01 |
| Gene Sequence |
| Protein sequence |
