AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380016133.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016133.01.T01 MTR_3g084140 100.000 73 0 0 1 73 1 73 7.15e-49 148
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016133.01.T01 AT5G42300 94.521 73 4 0 1 73 1 73 1.11e-45 140
MsG0380016133.01.T01 AT3G45180 90.411 73 7 0 1 73 1 73 2.28e-44 137
MsG0380016133.01.T01 AT3G45180 90.411 73 7 0 1 73 1 73 2.28e-44 137

Find 22 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATCTTGTCAGCCCTGGTTC+CGG 0.386948 3:-79473696 None:intergenic
TGGTGTACCACTTCTGAATT+CGG 0.396732 3:-79473718 None:intergenic
TGGCTGCTCAGACCGGAACC+AGG 0.440186 3:+79473684 MsG0380016133.01.T01:CDS
GTTGTTCTGAACGATCGATT+GGG 0.442659 3:+79473602 MsG0380016133.01.T01:CDS
AATTCGGATCTTGTCAGCCC+TGG 0.475569 3:-79473702 None:intergenic
GGTTGTTCTGAACGATCGAT+TGG 0.506700 3:+79473601 MsG0380016133.01.T01:CDS
GAACGATCGATTGGGGAAGA+AGG 0.524636 3:+79473610 MsG0380016133.01.T01:CDS
AAGCTGGTGGCTGCTCAGAC+CGG 0.532945 3:+79473677 MsG0380016133.01.T01:CDS
AGTGATGTGATCCTTGTAGA+TGG 0.540415 3:-79473738 None:intergenic
GTTCAAGACCCATGCCATCA+TGG 0.543744 3:-79473778 None:intergenic
GATCGGTGATTTGAAGAAGC+TGG 0.556890 3:+79473661 MsG0380016133.01.T01:CDS
TACAAGGATCACATCACTCT+CGG 0.558076 3:+79473743 MsG0380016133.01.T01:CDS
GGCTGCTCAGACCGGAACCA+GGG 0.582375 3:+79473685 MsG0380016133.01.T01:CDS
TACGAGATCCATGATGGCAT+GGG 0.593857 3:+79473770 MsG0380016133.01.T01:CDS
CTACGCTACGAAGATGATTG+AGG 0.594624 3:+79473580 None:intergenic
TTACGAGATCCATGATGGCA+TGG 0.599697 3:+79473769 MsG0380016133.01.T01:CDS
TTGTTCTGAACGATCGATTG+GGG 0.606269 3:+79473603 MsG0380016133.01.T01:CDS
GGTGATTACGAGATCCATGA+TGG 0.610211 3:+79473764 MsG0380016133.01.T01:CDS
GAAGTGGTACACCATCTACA+AGG 0.616793 3:+79473727 MsG0380016133.01.T01:CDS
ACAAGATCCGAATTCAGAAG+TGG 0.640248 3:+79473711 MsG0380016133.01.T01:CDS
TGCAACTCAGATGACACGAT+CGG 0.661294 3:+79473644 MsG0380016133.01.T01:CDS
CGGTGATTTGAAGAAGCTGG+TGG 0.708874 3:+79473664 MsG0380016133.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ACAAGATCCGAATTCAGAAG+TGG + Chr3:79473711-79473730 MsG0380016133.01.T01:CDS 40.0%
AGTGATGTGATCCTTGTAGA+TGG - Chr3:79473741-79473760 None:intergenic 40.0%
TACAAGGATCACATCACTCT+CGG + Chr3:79473743-79473762 MsG0380016133.01.T01:CDS 40.0%
TGGTGTACCACTTCTGAATT+CGG - Chr3:79473721-79473740 None:intergenic 40.0%
! GTTGTTCTGAACGATCGATT+GGG + Chr3:79473602-79473621 MsG0380016133.01.T01:CDS 40.0%
! TTGTTCTGAACGATCGATTG+GGG + Chr3:79473603-79473622 MsG0380016133.01.T01:CDS 40.0%
GAAGTGGTACACCATCTACA+AGG + Chr3:79473727-79473746 MsG0380016133.01.T01:CDS 45.0%
GATCGGTGATTTGAAGAAGC+TGG + Chr3:79473661-79473680 MsG0380016133.01.T01:CDS 45.0%
GGTGATTACGAGATCCATGA+TGG + Chr3:79473764-79473783 MsG0380016133.01.T01:CDS 45.0%
TACGAGATCCATGATGGCAT+GGG + Chr3:79473770-79473789 MsG0380016133.01.T01:CDS 45.0%
TGCAACTCAGATGACACGAT+CGG + Chr3:79473644-79473663 MsG0380016133.01.T01:CDS 45.0%
TTACGAGATCCATGATGGCA+TGG + Chr3:79473769-79473788 MsG0380016133.01.T01:CDS 45.0%
! GGTTGTTCTGAACGATCGAT+TGG + Chr3:79473601-79473620 MsG0380016133.01.T01:CDS 45.0%
AATTCGGATCTTGTCAGCCC+TGG - Chr3:79473705-79473724 None:intergenic 50.0%
CGGTGATTTGAAGAAGCTGG+TGG + Chr3:79473664-79473683 MsG0380016133.01.T01:CDS 50.0%
GAACGATCGATTGGGGAAGA+AGG + Chr3:79473610-79473629 MsG0380016133.01.T01:CDS 50.0%
GTTCAAGACCCATGCCATCA+TGG - Chr3:79473781-79473800 None:intergenic 50.0%
GATCTTGTCAGCCCTGGTTC+CGG - Chr3:79473699-79473718 None:intergenic 55.0%
AAGCTGGTGGCTGCTCAGAC+CGG + Chr3:79473677-79473696 MsG0380016133.01.T01:CDS 60.0%
GGCTGCTCAGACCGGAACCA+GGG + Chr3:79473685-79473704 MsG0380016133.01.T01:CDS 65.0%
TGGCTGCTCAGACCGGAACC+AGG + Chr3:79473684-79473703 MsG0380016133.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr3 gene 79473593 79473814 79473593 ID=MsG0380016133.01;Name=MsG0380016133.01
Chr3 mRNA 79473593 79473814 79473593 ID=MsG0380016133.01.T01;Parent=MsG0380016133.01;Name=MsG0380016133.01.T01;_AED=0.46;_eAED=0.46;_QI=0|-1|0|1|-1|1|1|0|73
Chr3 exon 79473593 79473814 79473593 ID=MsG0380016133.01.T01:exon:5140;Parent=MsG0380016133.01.T01
Chr3 CDS 79473593 79473814 79473593 ID=MsG0380016133.01.T01:cds;Parent=MsG0380016133.01.T01
Gene Sequence

>MsG0380016133.01.T01

ATGATTGAGGTTGTTCTGAACGATCGATTGGGGAAGAAGGTTCGCGTGAAGTGCAACTCAGATGACACGATCGGTGATTTGAAGAAGCTGGTGGCTGCTCAGACCGGAACCAGGGCTGACAAGATCCGAATTCAGAAGTGGTACACCATCTACAAGGATCACATCACTCTCGGTGATTACGAGATCCATGATGGCATGGGTCTTGAACTCTACTACAACTAA

Protein sequence

>MsG0380016133.01.T01

MIEVVLNDRLGKKVRVKCNSDDTIGDLKKLVAAQTGTRADKIRIQKWYTIYKDHITLGDYEIHDGMGLELYYN*