AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280010953.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010953.01.T01 MTR_2g095730 94.347 513 24 1 1 513 1 508 0.0 1006
MsG0280010953.01.T01 MTR_2g095750 79.644 506 91 5 1 501 1 499 0.0 824
MsG0280010953.01.T01 MTR_2g095710 80.240 501 80 6 1 501 1 482 0.0 799
MsG0280010953.01.T01 MTR_4g057585 64.240 467 153 7 43 501 34 494 0.0 615
MsG0280010953.01.T01 MTR_1g094155 59.681 501 188 6 22 513 5 500 0.0 611
MsG0280010953.01.T01 MTR_4g057595 63.948 466 156 7 42 502 38 496 0.0 610
MsG0280010953.01.T01 MTR_4g057600 61.522 473 172 6 34 502 28 494 0.0 592
MsG0280010953.01.T01 MTR_1g050545 63.816 456 155 7 51 501 32 482 0.0 575
MsG0280010953.01.T01 MTR_4g057570 56.448 473 189 10 37 501 28 491 4.62e-178 509
MsG0280010953.01.T01 MTR_5g097180 32.401 429 265 10 90 508 32 445 3.31e-66 221
MsG0280010953.01.T01 MTR_4g084710 32.609 414 256 8 95 500 39 437 1.04e-63 214
MsG0280010953.01.T01 MTR_3g079560 30.303 429 255 14 99 508 92 495 9.92e-51 181
MsG0280010953.01.T01 MTR_3g079570 29.213 445 278 12 77 508 75 495 6.58e-50 179
MsG0280010953.01.T01 MTR_3g079590 29.345 443 281 12 77 508 74 495 8.76e-50 178
MsG0280010953.01.T01 MTR_1g020090 29.712 451 275 13 69 501 69 495 7.38e-49 176
MsG0280010953.01.T01 MTR_3g434850 30.233 430 250 13 99 500 44 451 4.04e-48 173
MsG0280010953.01.T01 MTR_2g063000 27.193 456 290 14 71 499 19 459 3.33e-47 171
MsG0280010953.01.T01 MTR_3g079600 27.220 518 327 15 9 504 3 492 2.32e-46 169
MsG0280010953.01.T01 MTR_1g021010 29.524 420 254 13 99 501 90 484 7.49e-46 167
MsG0280010953.01.T01 MTR_2g081620 28.169 426 260 12 99 499 55 459 1.52e-45 166
MsG0280010953.01.T01 MTR_2g062990 27.123 424 267 12 99 499 54 458 1.66e-45 166
MsG0280010953.01.T01 MTR_2g018590 27.765 443 253 15 101 500 49 467 2.07e-45 166
MsG0280010953.01.T01 MTR_8g011350 27.158 475 269 11 85 499 35 492 3.07e-45 166
MsG0280010953.01.T01 MTR_7g021130 28.873 426 260 16 101 500 46 454 4.11e-45 165
MsG0280010953.01.T01 MTR_3g034290 28.810 420 265 13 96 499 85 486 4.14e-45 166
MsG0280010953.01.T01 MTR_8g099930 26.698 427 274 14 99 500 47 459 7.27e-45 164
MsG0280010953.01.T01 MTR_2g062980 26.747 415 280 11 99 499 54 458 2.49e-44 163
MsG0280010953.01.T01 MTR_3g079630 27.376 526 323 18 9 504 3 499 3.16e-44 163
MsG0280010953.01.T01 MTR_1g021020 28.502 414 265 10 99 501 92 485 3.96e-44 162
MsG0280010953.01.T01 MTR_7g051430 26.350 463 265 14 99 499 44 492 1.47e-43 161
MsG0280010953.01.T01 MTR_3g498755 27.336 428 267 14 101 500 31 442 1.66e-43 160
MsG0280010953.01.T01 MTR_4g122950 26.667 435 275 14 97 499 51 473 1.99e-43 160
MsG0280010953.01.T01 MTR_3g079620 29.241 448 269 13 77 501 74 496 2.29e-43 161
MsG0280010953.01.T01 MTR_4g122960 26.512 430 280 12 97 499 51 471 2.55e-43 160
MsG0280010953.01.T01 MTR_7g051430 35.686 255 145 5 99 348 44 284 2.64e-43 159
MsG0280010953.01.T01 MTR_7g080180 35.766 274 153 8 84 348 31 290 3.68e-42 157
MsG0280010953.01.T01 MTR_1g095560 29.314 423 262 16 99 500 47 453 4.46e-42 156
MsG0280010953.01.T01 MTR_7g080180 35.766 274 153 8 84 348 31 290 8.45e-42 156
MsG0280010953.01.T01 MTR_2g018600 25.866 433 273 13 101 500 50 467 1.94e-41 155
MsG0280010953.01.T01 MTR_0003s0560 28.979 421 271 14 91 499 79 483 2.84e-41 155
MsG0280010953.01.T01 MTR_4g075150 29.691 421 267 15 99 500 48 458 3.11e-41 154
MsG0280010953.01.T01 MTR_2g018630 27.674 430 262 15 101 500 53 463 1.22e-40 152
MsG0280010953.01.T01 MTR_4g089155 25.986 431 281 12 96 500 64 482 1.60e-40 152
MsG0280010953.01.T01 MTR_4g075150 29.551 423 267 15 99 500 48 460 1.70e-40 152
MsG0280010953.01.T01 MTR_8g011350 38.356 219 125 4 85 298 35 248 7.16e-40 150
MsG0280010953.01.T01 MTR_1g095540 27.725 422 270 13 99 500 47 453 1.10e-39 150
MsG0280010953.01.T01 MTR_1g095560 28.222 450 264 18 99 500 47 485 1.26e-39 150
MsG0280010953.01.T01 MTR_2g449850 25.968 439 267 15 94 500 47 459 3.76e-39 148
MsG0280010953.01.T01 MTR_3g090130 26.608 451 271 13 91 500 22 453 3.78e-39 148
MsG0280010953.01.T01 MTR_1g040370 27.913 412 240 16 101 500 46 412 9.62e-39 146
MsG0280010953.01.T01 MTR_4g021535 28.502 414 250 15 113 497 65 461 1.79e-38 146
MsG0280010953.01.T01 MTR_2g012850 26.402 428 275 13 99 500 49 462 2.65e-38 146
MsG0280010953.01.T01 MTR_4g021530 28.434 415 249 15 113 497 69 465 3.33e-38 146
MsG0280010953.01.T01 MTR_4g021510 27.966 472 282 16 57 496 18 463 5.33e-38 145
MsG0280010953.01.T01 MTR_5g072570 25.822 426 288 14 96 500 40 458 7.22e-38 145
MsG0280010953.01.T01 MTR_7g013790 26.316 437 259 15 101 500 51 461 9.33e-38 144
MsG0280010953.01.T01 MTR_2g012860 25.917 436 285 13 91 500 47 470 1.55e-37 144
MsG0280010953.01.T01 MTR_3g100420 25.442 452 282 10 84 497 57 491 6.77e-37 142
MsG0280010953.01.T01 MTR_3g090150 25.647 425 288 9 96 500 44 460 1.49e-36 141
MsG0280010953.01.T01 MTR_4g021520 27.842 431 256 18 101 497 56 465 2.76e-36 140
MsG0280010953.01.T01 MTR_3g045770 27.332 461 276 17 72 501 21 453 9.48e-36 139
MsG0280010953.01.T01 MTR_3g034290 28.783 337 212 11 96 417 85 408 1.76e-35 137
MsG0280010953.01.T01 MTR_4g021490 27.295 414 255 14 113 497 69 465 3.89e-35 137
MsG0280010953.01.T01 MTR_4g021500 26.697 442 252 15 113 512 69 480 8.16e-35 139
MsG0280010953.01.T01 MTR_4g021500 37.696 191 111 5 113 298 504 691 9.74e-31 127
MsG0280010953.01.T01 MTR_4g021500 26.471 442 253 14 113 512 69 480 8.70e-35 139
MsG0280010953.01.T01 MTR_4g021500 25.943 424 230 13 113 497 504 882 1.66e-34 138
MsG0280010953.01.T01 MTR_2g063000 24.449 454 274 14 71 499 19 428 5.10e-34 134
MsG0280010953.01.T01 MTR_4g021520 39.512 205 113 7 101 298 56 256 1.72e-32 129
MsG0280010953.01.T01 MTR_0003s0560 34.483 203 129 4 91 291 79 279 1.63e-31 125
MsG0280010953.01.T01 MTR_2g009200 25.000 472 253 19 71 498 21 435 7.37e-29 119
MsG0280010953.01.T01 MTR_4g056520 25.587 426 263 12 112 498 44 454 9.99e-28 116
MsG0280010953.01.T01 MTR_2g084530 28.266 421 246 16 112 493 44 447 1.29e-27 115
MsG0280010953.01.T01 MTR_2g018630 24.932 365 230 13 161 500 13 358 1.30e-23 102
MsG0280010953.01.T01 MTR_4g021535 24.396 414 220 17 113 497 65 414 2.41e-19 90.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010953.01.T01 AT3G45010 60.000 510 189 7 1 503 1 502 0.0 619
MsG0280010953.01.T01 AT3G10410 59.880 501 179 8 13 501 6 496 0.0 618
MsG0280010953.01.T01 AT5G22980 60.521 499 179 7 17 504 7 498 0.0 602
MsG0280010953.01.T01 AT5G22960 61.310 168 44 3 93 260 28 174 3.09e-65 210
MsG0280010953.01.T01 AT1G15000 31.159 414 257 11 95 500 31 424 4.70e-53 186
MsG0280010953.01.T01 AT2G22920 30.631 444 264 18 71 499 18 432 5.42e-53 186
MsG0280010953.01.T01 AT2G22970 29.663 445 267 18 71 500 18 431 2.52e-52 184
MsG0280010953.01.T01 AT1G73300 29.864 442 270 16 71 500 26 439 1.93e-50 179
MsG0280010953.01.T01 AT1G73300 29.864 442 270 16 71 500 26 439 1.93e-50 179
MsG0280010953.01.T01 AT3G25420 29.046 482 255 17 84 500 33 492 1.96e-49 177
MsG0280010953.01.T01 AT2G22920 32.292 384 223 15 71 440 18 378 5.31e-49 174
MsG0280010953.01.T01 AT3G12203 26.683 416 265 13 100 500 24 414 5.78e-49 174
MsG0280010953.01.T01 AT4G12910 27.917 480 259 15 84 499 38 494 7.11e-49 176
MsG0280010953.01.T01 AT2G22970 31.771 384 223 15 71 440 18 376 8.26e-49 174
MsG0280010953.01.T01 AT3G12203 26.683 416 265 13 100 500 45 435 1.19e-48 174
MsG0280010953.01.T01 AT2G22970 29.561 433 260 17 71 488 18 420 1.28e-48 174
MsG0280010953.01.T01 AT3G12203 27.114 402 253 13 114 500 1 377 3.53e-48 171
MsG0280010953.01.T01 AT2G23010 29.176 449 268 18 71 500 18 435 3.63e-48 172
MsG0280010953.01.T01 AT5G36180 28.959 442 274 15 71 500 26 439 5.37e-48 172
MsG0280010953.01.T01 AT2G22980 28.798 441 272 18 71 500 19 428 6.17e-48 172
MsG0280010953.01.T01 AT2G23010 29.176 449 268 18 71 500 18 435 1.38e-47 171
MsG0280010953.01.T01 AT1G73290 27.765 443 276 15 71 500 25 436 2.05e-47 171
MsG0280010953.01.T01 AT1G73270 27.314 443 280 15 71 500 26 439 2.47e-47 170
MsG0280010953.01.T01 AT1G73280 27.828 442 279 14 71 500 26 439 2.54e-47 170
MsG0280010953.01.T01 AT1G73270 27.314 443 280 15 71 500 26 439 3.48e-47 170
MsG0280010953.01.T01 AT3G12240 28.777 417 256 16 100 500 43 434 7.53e-47 169
MsG0280010953.01.T01 AT3G10450 28.140 430 272 14 83 500 31 435 1.09e-46 169
MsG0280010953.01.T01 AT3G10450 27.561 410 264 12 103 500 4 392 1.59e-45 164
MsG0280010953.01.T01 AT1G73290 29.581 382 232 12 71 440 25 381 2.94e-45 164
MsG0280010953.01.T01 AT3G12220 26.794 418 265 14 100 501 42 434 4.60e-45 164
MsG0280010953.01.T01 AT2G22990 28.381 451 267 18 71 500 16 431 5.82e-45 164
MsG0280010953.01.T01 AT2G22980 28.947 418 256 17 71 477 19 406 7.21e-45 163
MsG0280010953.01.T01 AT2G22980 28.505 428 265 17 71 487 19 416 8.15e-45 163
MsG0280010953.01.T01 AT3G12230 28.058 417 259 15 100 500 42 433 8.92e-45 163
MsG0280010953.01.T01 AT3G17180 27.991 443 261 15 96 500 50 472 9.36e-45 164
MsG0280010953.01.T01 AT4G12910 32.537 335 197 11 84 407 38 354 1.27e-44 163
MsG0280010953.01.T01 AT3G12220 27.160 405 254 14 113 501 25 404 2.30e-44 161
MsG0280010953.01.T01 AT4G15100 27.252 433 270 13 99 499 58 477 2.45e-44 163
MsG0280010953.01.T01 AT1G73310 28.281 442 277 15 71 500 26 439 2.95e-44 162
MsG0280010953.01.T01 AT5G23210 28.246 439 267 14 99 500 65 492 3.54e-44 163
MsG0280010953.01.T01 AT4G30810 26.218 431 268 12 97 499 50 458 4.08e-44 162
MsG0280010953.01.T01 AT5G08260 30.162 431 248 17 99 493 53 466 5.29e-44 162
MsG0280010953.01.T01 AT2G23000 28.029 421 256 15 101 500 41 435 1.02e-43 160
MsG0280010953.01.T01 AT2G23000 28.029 421 256 15 101 500 41 435 1.02e-43 160
MsG0280010953.01.T01 AT2G22920 34.921 315 176 12 71 376 18 312 1.17e-43 157
MsG0280010953.01.T01 AT1G28110 27.294 425 270 13 99 499 45 454 1.27e-43 160
MsG0280010953.01.T01 AT1G28110 27.294 425 270 13 99 499 45 454 1.27e-43 160
MsG0280010953.01.T01 AT1G61130 28.774 424 267 14 99 499 45 456 3.72e-43 159
MsG0280010953.01.T01 AT1G11080 27.650 434 269 13 99 499 64 485 5.59e-43 159
MsG0280010953.01.T01 AT1G11080 27.650 434 269 13 99 499 64 485 6.56e-43 159
MsG0280010953.01.T01 AT2G33530 27.739 429 267 13 99 500 47 459 1.77e-42 157
MsG0280010953.01.T01 AT3G07990 28.604 437 253 16 96 500 45 454 2.74e-42 157
MsG0280010953.01.T01 AT3G52000 28.780 410 269 13 99 498 78 474 4.02e-42 157
MsG0280010953.01.T01 AT4G30610 27.083 432 271 14 97 500 44 459 7.45e-42 156
MsG0280010953.01.T01 AT3G10450 27.764 407 258 13 83 477 31 413 7.96e-42 155
MsG0280010953.01.T01 AT2G22970 34.069 317 181 12 71 379 18 314 8.65e-42 152
MsG0280010953.01.T01 AT2G35770 28.148 405 263 10 101 490 54 445 1.35e-41 155
MsG0280010953.01.T01 AT1G73290 36.596 235 134 7 71 299 25 250 1.69e-41 151
MsG0280010953.01.T01 AT1G33540 26.207 435 269 14 101 500 45 462 2.32e-41 154
MsG0280010953.01.T01 AT2G24010 28.736 435 272 13 84 500 34 448 2.72e-41 154
MsG0280010953.01.T01 AT5G23210 40.686 204 116 5 99 298 65 267 3.12e-41 154
MsG0280010953.01.T01 AT3G63470 29.298 413 254 16 99 493 95 487 3.93e-41 155
MsG0280010953.01.T01 AT2G22990 29.091 440 239 19 71 479 16 413 7.14e-41 152
MsG0280010953.01.T01 AT2G22990 29.128 436 238 19 71 477 16 409 1.03e-40 152
MsG0280010953.01.T01 AT3G10450 27.132 387 250 11 103 477 4 370 1.07e-40 150
MsG0280010953.01.T01 AT1G33540 26.097 433 268 14 103 500 4 419 1.19e-40 152
MsG0280010953.01.T01 AT1G73310 29.504 383 233 13 71 440 26 384 1.35e-40 152
MsG0280010953.01.T01 AT3G52020 26.562 512 315 18 17 498 13 493 5.51e-40 151
MsG0280010953.01.T01 AT3G52010 27.315 432 277 14 84 498 68 479 1.10e-39 150
MsG0280010953.01.T01 AT2G35780 27.123 424 272 13 97 500 42 448 1.29e-39 149
MsG0280010953.01.T01 AT2G24000 27.394 449 268 17 84 499 34 457 5.92e-39 148
MsG0280010953.01.T01 AT2G22980 33.544 316 176 12 71 376 19 310 6.91e-39 145
MsG0280010953.01.T01 AT1G73280 36.481 233 135 6 71 298 26 250 1.76e-38 145
MsG0280010953.01.T01 AT2G22990 27.051 451 264 19 71 500 16 422 6.99e-38 144
MsG0280010953.01.T01 AT5G09640 26.124 467 286 19 71 500 19 463 7.28e-38 145
MsG0280010953.01.T01 AT1G43780 28.604 437 253 17 101 500 53 467 8.66e-38 145
MsG0280010953.01.T01 AT2G12480 27.294 425 272 15 84 500 35 430 6.76e-37 142
MsG0280010953.01.T01 AT2G24010 27.556 450 273 14 84 500 34 463 1.19e-36 142
MsG0280010953.01.T01 AT3G17180 33.469 245 143 8 96 332 50 282 1.87e-36 140
MsG0280010953.01.T01 AT2G12480 26.376 436 286 15 84 500 35 454 1.92e-36 141
MsG0280010953.01.T01 AT2G12480 27.529 425 269 16 84 500 35 428 6.02e-36 139
MsG0280010953.01.T01 AT3G12220 34.951 206 128 3 100 300 42 246 7.83e-36 137
MsG0280010953.01.T01 AT5G42230 26.950 423 281 13 99 500 42 457 1.98e-35 138
MsG0280010953.01.T01 AT5G42230 26.950 423 281 13 99 500 42 457 1.98e-35 138
MsG0280010953.01.T01 AT3G02110 25.455 440 264 13 101 500 53 468 6.47e-35 137
MsG0280010953.01.T01 AT2G12480 27.465 426 269 16 84 500 35 429 9.20e-35 136
MsG0280010953.01.T01 AT5G42240 25.463 432 276 14 99 500 46 461 1.51e-34 135
MsG0280010953.01.T01 AT2G05850 25.664 452 291 16 67 498 53 479 1.82e-34 135
MsG0280010953.01.T01 AT5G23210 27.431 401 244 13 136 500 8 397 2.94e-34 134
MsG0280010953.01.T01 AT5G23210 27.250 400 244 13 137 500 8 396 1.61e-33 131
MsG0280010953.01.T01 AT2G24010 26.383 470 278 16 84 500 5 459 1.47e-32 130
MsG0280010953.01.T01 AT5G23210 41.212 165 93 4 137 298 8 171 1.06e-30 123
MsG0280010953.01.T01 AT5G36180 26.801 347 226 10 161 500 12 337 1.28e-30 122
MsG0280010953.01.T01 AT5G36180 26.801 347 226 10 161 500 12 337 1.28e-30 122
MsG0280010953.01.T01 AT1G73280 25.648 347 230 9 161 500 12 337 2.01e-30 121
MsG0280010953.01.T01 AT2G27920 26.588 425 252 14 112 493 45 452 2.86e-30 123
MsG0280010953.01.T01 AT3G56540 40.845 142 80 4 99 238 93 232 9.84e-29 115
MsG0280010953.01.T01 AT2G22990 26.705 352 198 14 173 500 2 317 2.61e-28 115
MsG0280010953.01.T01 AT2G24010 28.686 373 204 15 84 436 5 335 3.78e-28 115
MsG0280010953.01.T01 AT2G22990 26.705 352 198 14 173 500 2 317 7.23e-28 114
MsG0280010953.01.T01 AT5G36180 26.235 324 212 9 161 477 12 315 1.96e-26 110
MsG0280010953.01.T01 AT5G36180 27.972 286 185 7 161 440 12 282 5.82e-26 108
MsG0280010953.01.T01 AT1G73280 24.324 333 224 9 175 500 1 312 1.17e-25 107
MsG0280010953.01.T01 AT2G22980 25.723 311 202 11 173 477 2 289 5.78e-25 105
MsG0280010953.01.T01 AT1G73310 26.389 288 187 7 161 440 12 282 3.61e-24 103
MsG0280010953.01.T01 AT2G27920 25.124 402 233 15 135 493 4 380 6.71e-24 103
MsG0280010953.01.T01 AT2G27920 24.425 348 217 10 182 493 48 385 1.44e-17 85.1

Find 120 sgRNAs with CRISPR-Local

Find 184 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCATTCTCTGGTATGTTTA+TGG 0.137565 2:+77589404 None:intergenic
ACAACATGGCATCATCTTTA+AGG 0.142828 2:-77589593 None:intergenic
ATACAACAAGAAAGTGATTT+TGG 0.209981 2:+77586826 None:intergenic
TATGCCATGTACTGAATTTC+AGG 0.244869 2:+77588042 None:intergenic
TCCCTTGTTTGGAATGAATA+TGG 0.250663 2:-77588835 MsG0280010953.01.T01:CDS
GTCAGGTCAGACAACATTTA+AGG 0.308088 2:-77587223 MsG0280010953.01.T01:CDS
TGGACCTTTGGCCTTCCTTA+AGG 0.320499 2:-77587142 MsG0280010953.01.T01:intron
ACAAGAACTTGTCCCTTGTT+TGG 0.329032 2:-77588846 MsG0280010953.01.T01:CDS
AATAAACAGGGCTTTGCTAT+TGG 0.339075 2:-77588081 MsG0280010953.01.T01:intron
CACTTCAGATGTTACAAGAT+TGG 0.348561 2:-77586878 MsG0280010953.01.T01:CDS
CGATTTGTATGACTTCTTGC+AGG 0.353476 2:-77588603 MsG0280010953.01.T01:intron
CAGGGCTTTGCTATTGGTAA+TGG 0.354719 2:-77588075 MsG0280010953.01.T01:CDS
CTCATTTGGTAGGGAATTCA+AGG 0.357235 2:-77587266 MsG0280010953.01.T01:intron
TCTGAAAAGCCATGGACCTT+TGG 0.366511 2:-77587154 MsG0280010953.01.T01:CDS
AGGCAATGGACTGATTAATA+AGG 0.371408 2:-77588004 MsG0280010953.01.T01:CDS
AAACATTTACCTAGCAGCTT+TGG 0.372493 2:+77589263 None:intergenic
TAAAGGAAGCTTTAGGTGTT+GGG 0.376899 2:-77587514 MsG0280010953.01.T01:CDS
ACAGCATACATTAGTGTTGC+CGG 0.385275 2:+77587720 None:intergenic
CCTGTCGTCATATGGTTAAC+TGG 0.391322 2:-77588934 MsG0280010953.01.T01:CDS
GGTTATTCTGTTGAAGAACT+TGG 0.406042 2:-77589318 MsG0280010953.01.T01:CDS
TGTCTGACCTGACCACTTCA+TGG 0.416219 2:+77587233 None:intergenic
CTTAGTACACGCTGCCTTGA+CGG 0.424079 2:-77587465 MsG0280010953.01.T01:CDS
AAGAAATGTGAGGGAGCTTT+CGG 0.426372 2:-77587587 MsG0280010953.01.T01:CDS
TTGTCCTTTGAGAACTGAAA+AGG 0.434846 2:+77588865 None:intergenic
GGGAGCTTTCGGTGATTGTC+AGG 0.436170 2:-77587576 MsG0280010953.01.T01:CDS
GGCTGGTCACATGGTTCCTA+TGG 0.441603 2:-77586916 MsG0280010953.01.T01:CDS
GTAGACCAACCCATTGGATC+AGG 0.443437 2:-77588686 MsG0280010953.01.T01:CDS
TTTGTTTCCTTGGTGAACTC+TGG 0.443497 2:+77588248 None:intergenic
AGTCCTCTTATGAGAGTTGA+AGG 0.448595 2:+77589447 None:intergenic
GAGAAGAAGTTCACTTTCCC+TGG 0.449586 2:-77589354 MsG0280010953.01.T01:CDS
CTTTGAATCTCGAAACAGTA+AGG 0.449789 2:-77588962 MsG0280010953.01.T01:CDS
TCCCCTTCAACTCTCATAAG+AGG 0.452569 2:-77589450 MsG0280010953.01.T01:CDS
ACGCTGCCTTGACGGAAGAT+TGG 0.455836 2:-77587457 MsG0280010953.01.T01:CDS
AACATCTGAAGTGCAACTTT+TGG 0.458205 2:+77586888 None:intergenic
TTTGGGATGCAGTTGAAGAA+AGG 0.463192 2:+77589503 None:intergenic
AAGACAGTAAAGGAAGCTTT+AGG 0.467806 2:-77587521 MsG0280010953.01.T01:CDS
GCAATTTCACTGCCGCATCC+TGG 0.467824 2:+77588907 None:intergenic
AAGGGGATGCAGTTGAAGAA+AGG 0.469788 2:+77589466 None:intergenic
TGAACTCTGGATGCAAAAGC+TGG 0.474414 2:+77588261 None:intergenic
GCATATACAGATTATGCATT+AGG 0.474872 2:-77588024 MsG0280010953.01.T01:CDS
ATTCCTGCTCTTCTTGAGGA+TGG 0.475493 2:-77587413 MsG0280010953.01.T01:CDS
GAAGAACTTGGTCATCATGC+AGG 0.475724 2:-77589306 MsG0280010953.01.T01:CDS
TTTGGAACAATAGGTGAAAG+AGG 0.477010 2:-77586937 MsG0280010953.01.T01:intron
CATGTACTGAATTTCAGGAT+TGG 0.477634 2:+77588047 None:intergenic
GTAAAGGAAGCTTTAGGTGT+TGG 0.480724 2:-77587515 MsG0280010953.01.T01:CDS
TTGGTTGATAGTGAAGAAGC+AGG 0.485779 2:-77587180 MsG0280010953.01.T01:CDS
AGGAATTCATATAAATCTAA+AGG 0.501724 2:-77588217 MsG0280010953.01.T01:intron
TCTTCTCCACAATGTTTCCA+TGG 0.501980 2:+77589370 None:intergenic
TACCTAGCAGCTTTGGAATG+AGG 0.502116 2:+77589270 None:intergenic
CAATCCTGAAATTCAGTACA+TGG 0.506627 2:-77588046 MsG0280010953.01.T01:CDS
GGGTATTCCTGCTCTTCTTG+AGG 0.508272 2:-77587417 MsG0280010953.01.T01:CDS
ATTTATCTGTTAGGCACTGA+AGG 0.510266 2:-77587808 MsG0280010953.01.T01:intron
GTTGATCCCACCGTGTCTAA+AGG 0.511902 2:-77587959 MsG0280010953.01.T01:CDS
AATCATTCTTTGTGAACTGA+GGG 0.514863 2:+77588322 None:intergenic
CGCCGTGATGAAGACAGTAT+TGG 0.518941 2:-77588629 MsG0280010953.01.T01:CDS
CCAGTTAACCATATGACGAC+AGG 0.521891 2:+77588934 None:intergenic
CAAGGAAACAAAGCAAAAGA+AGG 0.523553 2:-77588237 MsG0280010953.01.T01:CDS
ATGAAGGTGAAATGAAGAAG+AGG 0.524790 2:+77589529 None:intergenic
ATTCCATCCTCAAGAAGAGC+AGG 0.526347 2:+77587410 None:intergenic
AGGATGACCCTGTCGTCATA+TGG 0.526809 2:-77588942 MsG0280010953.01.T01:CDS
AGGACATGAGGATCATTCTC+TGG 0.531938 2:+77589393 None:intergenic
GAACAATAGGTGAAAGAGGC+TGG 0.533723 2:-77586933 MsG0280010953.01.T01:intron
GATCTAATATGCAATTGGCT+TGG 0.534093 2:-77587359 MsG0280010953.01.T01:intron
ATGTTACAAGATTGGATGCA+AGG 0.542295 2:-77586870 MsG0280010953.01.T01:CDS
ATGTCCAGCGTATGACTCTC+CGG 0.542492 2:+77588290 None:intergenic
ATATGGTTAACTGGAGGTCC+AGG 0.542845 2:-77588925 MsG0280010953.01.T01:CDS
GTTGATGCCATGAAGTGGTC+AGG 0.543073 2:-77587240 MsG0280010953.01.T01:CDS
CTTGAACATGAAGACAGTAA+AGG 0.545435 2:-77587531 MsG0280010953.01.T01:CDS
AAATCATTCTTTGTGAACTG+AGG 0.546581 2:+77588321 None:intergenic
ATATTTGTAGACCAACCCAT+TGG 0.549517 2:-77588692 MsG0280010953.01.T01:CDS
TTGTTTGGAATGAATATGGA+TGG 0.552020 2:-77588831 MsG0280010953.01.T01:CDS
ATAGCTGAAACCTGATCCAA+TGG 0.556117 2:+77588676 None:intergenic
CTGCCGCCTTTAGACACGGT+GGG 0.565722 2:+77587953 None:intergenic
GTCCTCTTATGAGAGTTGAA+GGG 0.568698 2:+77589448 None:intergenic
TCCTCTTATGAGAGTTGAAG+GGG 0.569189 2:+77589449 None:intergenic
AAAGGAAGCTTTAGGTGTTG+GGG 0.573810 2:-77587513 MsG0280010953.01.T01:CDS
AGTTGAAGAAAGGAGAATGA+AGG 0.577019 2:+77589513 None:intergenic
AATGATCCTCATGTCCTCCA+TGG 0.578894 2:-77589387 MsG0280010953.01.T01:CDS
TGTTACAAGATTGGATGCAA+GGG 0.580202 2:-77586869 MsG0280010953.01.T01:CDS
GCAGGAATTCTGAAAAGCCA+TGG 0.580565 2:-77587162 MsG0280010953.01.T01:CDS
ACAGATTATGCATTAGGCAA+TGG 0.580565 2:-77588018 MsG0280010953.01.T01:CDS
TTAACTGGAGGTCCAGGATG+CGG 0.584480 2:-77588919 MsG0280010953.01.T01:CDS
GACATTAGAAAGAAATGTGA+GGG 0.586086 2:-77587596 MsG0280010953.01.T01:CDS
GGTGAAAGAGGCTGGTCACA+TGG 0.589518 2:-77586925 MsG0280010953.01.T01:CDS
TTTGGTAGGGAATTCAAGGT+GGG 0.590648 2:-77587262 MsG0280010953.01.T01:intron
TAAGGAAGGCCAAAGGTCCA+TGG 0.590847 2:+77587145 None:intergenic
TTAAACACAAACCTTAAGGA+AGG 0.596840 2:+77587131 None:intergenic
ACTGAAGGTGGACAAGCATG+TGG 0.599485 2:-77587793 MsG0280010953.01.T01:CDS
ATCCATATTCATTCCAAACA+AGG 0.604369 2:+77588833 None:intergenic
TTCCTCATTCCAAAGCTGCT+AGG 0.605027 2:-77589272 MsG0280010953.01.T01:intron
TAACCAGAGTCATCAAAACC+AGG 0.605492 2:+77589336 None:intergenic
TTTCATCCAATCTTCCGTCA+AGG 0.611684 2:+77587451 None:intergenic
TAGCTGAAACCTGATCCAAT+GGG 0.613889 2:+77588677 None:intergenic
TGAAACCTGATCCAATGGGT+TGG 0.614257 2:+77588681 None:intergenic
TTGTCAGGACTTCTCTGACA+TGG 0.615587 2:-77587561 MsG0280010953.01.T01:CDS
GAATGAATATGGATGGGACA+AGG 0.625882 2:-77588824 MsG0280010953.01.T01:intron
GCGAATATCACTGTGAACAT+CGG 0.629348 2:+77588649 None:intergenic
ATGTTTCCATGGAGGACATG+AGG 0.630827 2:+77589381 None:intergenic
TATCTGTTAGGCACTGAAGG+TGG 0.631703 2:-77587805 MsG0280010953.01.T01:intron
TATCAGATCACAAACATCAC+CGG 0.631908 2:-77587739 MsG0280010953.01.T01:CDS
ATTTGGTAGGGAATTCAAGG+TGG 0.632416 2:-77587263 MsG0280010953.01.T01:intron
AAGAACTTGGTCATCATGCA+GGG 0.634335 2:-77589305 MsG0280010953.01.T01:CDS
TGTCCTCCATGGAAACATTG+TGG 0.634451 2:-77589376 MsG0280010953.01.T01:CDS
TCCATATTCATTCCAAACAA+GGG 0.638155 2:+77588834 None:intergenic
CGACATTAGAAAGAAATGTG+AGG 0.647229 2:-77587597 MsG0280010953.01.T01:CDS
AACCAGAGTCATCAAAACCA+GGG 0.649229 2:+77589337 None:intergenic
GTCGTCATATGGTTAACTGG+AGG 0.656929 2:-77588931 MsG0280010953.01.T01:CDS
ATCAAGTTACTTGTGTACGC+CGG 0.657584 2:-77587389 MsG0280010953.01.T01:CDS
CAGTTAACCATATGACGACA+GGG 0.657833 2:+77588935 None:intergenic
TGCCAATACTGTCTTCATCA+CGG 0.659820 2:+77588627 None:intergenic
GATCCCACCGTGTCTAAAGG+CGG 0.662660 2:-77587956 MsG0280010953.01.T01:CDS
CTGAAGGTGGACAAGCATGT+GGG 0.668943 2:-77587792 MsG0280010953.01.T01:CDS
TGTTTGGAATGAATATGGAT+GGG 0.672561 2:-77588830 MsG0280010953.01.T01:CDS
TCTCCACAATGTTTCCATGG+AGG 0.678885 2:+77589373 None:intergenic
TCTGCCGCCTTTAGACACGG+TGG 0.679091 2:+77587952 None:intergenic
ATTACCGGAGAGTCATACGC+TGG 0.680484 2:-77588294 MsG0280010953.01.T01:CDS
GGTGGGTTGATGCCATGAAG+TGG 0.684602 2:-77587245 MsG0280010953.01.T01:CDS
AATATCACTGTGAACATCGG+TGG 0.689654 2:+77588652 None:intergenic
TTTGCATCCAGAGTTCACCA+AGG 0.720470 2:-77588255 MsG0280010953.01.T01:CDS
CAAACCTTAAGGAAGGCCAA+AGG 0.724280 2:+77587138 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATATATATTTTTTTTTTTT+TGG - Chr2:77589457-77589476 MsG0280010953.01.T01:CDS 0.0%
!!! ATGTTTTTTATTTTTAAAAG+TGG - Chr2:77587281-77587300 MsG0280010953.01.T01:intron 10.0%
!! GAACTTTATAAAAGAAAAAT+GGG + Chr2:77587218-77587237 None:intergenic 15.0%
!! TGTTTATTATAAAAGATGAA+AGG + Chr2:77588319-77588338 None:intergenic 15.0%
!!! AAGAATGATTTTTATATTAC+CGG - Chr2:77588103-77588122 MsG0280010953.01.T01:intron 15.0%
!!! AATAAATTCCTCTAAAAAAA+AGG - Chr2:77587248-77587267 MsG0280010953.01.T01:CDS 15.0%
!!! ATTAATAACCTTTTTTTTAG+AGG + Chr2:77587259-77587278 None:intergenic 15.0%
!!! TTCATCTTTTATAATAAACA+GGG - Chr2:77588319-77588338 MsG0280010953.01.T01:CDS 15.0%
!!! TTTCATCTTTTATAATAAAC+AGG - Chr2:77588318-77588337 MsG0280010953.01.T01:CDS 15.0%
!!! TTTTTTTACGAAAAATTAAG+TGG - Chr2:77587899-77587918 MsG0280010953.01.T01:intron 15.0%
!!! TTTTTTTTTTTTGGAACAAT+AGG - Chr2:77589466-77589485 MsG0280010953.01.T01:CDS 15.0%
!! AAAAAGATCTAATATGCAAT+TGG - Chr2:77589048-77589067 MsG0280010953.01.T01:intron 20.0%
!! AGGAATTCATATAAATCTAA+AGG - Chr2:77588195-77588214 MsG0280010953.01.T01:intron 20.0%
!! ATAACTGTATATTCATACAA+AGG - Chr2:77588243-77588262 MsG0280010953.01.T01:CDS 20.0%
!! CGAACTTTATAAAAGAAAAA+TGG + Chr2:77587219-77587238 None:intergenic 20.0%
!!! ACTTAAATTTTTACAATGAC+AGG - Chr2:77588051-77588070 MsG0280010953.01.T01:CDS 20.0%
!!! ATAAATTAAGAGTGTCAATA+AGG + Chr2:77588590-77588609 None:intergenic 20.0%
!!! ATTTGTATCTTGTTTAAGAT+TGG + Chr2:77589097-77589116 None:intergenic 20.0%
!!! TATGTTATTTAGATTAAGTG+AGG - Chr2:77587331-77587350 MsG0280010953.01.T01:intron 20.0%
! AAATTGGTAAAATTCTCTCT+TGG + Chr2:77588542-77588561 None:intergenic 25.0%
! ACACTCTTAATTTATCTGTT+AGG - Chr2:77588595-77588614 MsG0280010953.01.T01:intron 25.0%
! ACAGACACTTCAAATTAAAA+GGG - Chr2:77589352-77589371 MsG0280010953.01.T01:CDS 25.0%
! ACATAATAATAATGTGTGCA+TGG - Chr2:77587374-77587393 MsG0280010953.01.T01:CDS 25.0%
! GATTAATCACTTGAATTAAG+TGG - Chr2:77587862-77587881 MsG0280010953.01.T01:intron 25.0%
! TACAGACACTTCAAATTAAA+AGG - Chr2:77589351-77589370 MsG0280010953.01.T01:CDS 25.0%
! TGACATAACAAATTGTTCAA+TGG + Chr2:77587408-77587427 None:intergenic 25.0%
! TTAGAACTAAAGTGGAAAAA+AGG + Chr2:77587638-77587657 None:intergenic 25.0%
!! AAAGTAGCACTAAAAGTATA+AGG - Chr2:77588724-77588743 MsG0280010953.01.T01:intron 25.0%
!! ATACAACAAGAAAGTGATTT+TGG + Chr2:77589589-77589608 None:intergenic 25.0%
!!! ATTGCCTTGTTTTATGAAAA+TGG - Chr2:77587523-77587542 MsG0280010953.01.T01:CDS 25.0%
!!! CTTTTTTCTTTCTCTTTTGT+AGG - Chr2:77587686-77587705 MsG0280010953.01.T01:intron 25.0%
!!! TATTTTTAAAAGTGGACAGA+CGG - Chr2:77587289-77587308 MsG0280010953.01.T01:intron 25.0%
!!! TTATGGAACTTTTAGGAAAA+AGG + Chr2:77586994-77587013 None:intergenic 25.0%
AAATCATTCTTTGTGAACTG+AGG + Chr2:77588094-77588113 None:intergenic 30.0%
AATCATTCTTTGTGAACTGA+GGG + Chr2:77588093-77588112 None:intergenic 30.0%
ACGACATTACATACTATCTA+TGG - Chr2:77587975-77587994 MsG0280010953.01.T01:CDS 30.0%
ATCATTCTCTGGTATGTTTA+TGG + Chr2:77587011-77587030 None:intergenic 30.0%
ATCCATATTCATTCCAAACA+AGG + Chr2:77587582-77587601 None:intergenic 30.0%
ATCCTTAAACACAAACCTTA+AGG + Chr2:77589288-77589307 None:intergenic 30.0%
GACATTAGAAAGAAATGTGA+GGG - Chr2:77588816-77588835 MsG0280010953.01.T01:intron 30.0%
GCACACATTATTATTATGTG+TGG + Chr2:77587373-77587392 None:intergenic 30.0%
GCATATACAGATTATGCATT+AGG - Chr2:77588388-77588407 MsG0280010953.01.T01:intron 30.0%
TAAAAAACAGACACACTTTG+AGG + Chr2:77586847-77586866 None:intergenic 30.0%
TCAACCAAAAATGAAGTTGT+AGG + Chr2:77589220-77589239 None:intergenic 30.0%
TCCATATTCATTCCAAACAA+GGG + Chr2:77587581-77587600 None:intergenic 30.0%
TGGATGAAAAATCTTGAAGT+GGG - Chr2:77588975-77588994 MsG0280010953.01.T01:CDS 30.0%
TGTATCAGTTAGAACTAAAG+TGG + Chr2:77587646-77587665 None:intergenic 30.0%
TTAAACACAAACCTTAAGGA+AGG + Chr2:77589284-77589303 None:intergenic 30.0%
TTCCTTAAGGTTTGTGTTTA+AGG - Chr2:77589283-77589302 MsG0280010953.01.T01:CDS 30.0%
TTGGATGAAAAATCTTGAAG+TGG - Chr2:77588974-77588993 MsG0280010953.01.T01:CDS 30.0%
! ACATGACTTGCACAATAAAT+TGG + Chr2:77588558-77588577 None:intergenic 30.0%
! AGGTCCATTTTCATAAAACA+AGG + Chr2:77587530-77587549 None:intergenic 30.0%
! GCATATTAGATCTTTTTCTC+CGG + Chr2:77589045-77589064 None:intergenic 30.0%
! GGTATGTTTATGGAACTTTT+AGG + Chr2:77587001-77587020 None:intergenic 30.0%
! TGTTTGGAATGAATATGGAT+GGG - Chr2:77587582-77587601 MsG0280010953.01.T01:CDS 30.0%
! TTGTTTGGAATGAATATGGA+TGG - Chr2:77587581-77587600 MsG0280010953.01.T01:CDS 30.0%
!! AAGGAGTATGTCATTGTTTT+TGG + Chr2:77586930-77586949 None:intergenic 30.0%
!! AGGAGTATGTCATTGTTTTT+GGG + Chr2:77586929-77586948 None:intergenic 30.0%
!! CAATTTGTTATGTCAATTGC+AGG - Chr2:77587413-77587432 MsG0280010953.01.T01:CDS 30.0%
AAACATTTACCTAGCAGCTT+TGG + Chr2:77587152-77587171 None:intergenic 35.0%
AAATGTGCTTCTCATTTGGT+AGG - Chr2:77589136-77589155 MsG0280010953.01.T01:intron 35.0%
AAGACAGTAAAGGAAGCTTT+AGG - Chr2:77588891-77588910 MsG0280010953.01.T01:CDS 35.0%
AATGTGCTTCTCATTTGGTA+GGG - Chr2:77589137-77589156 MsG0280010953.01.T01:intron 35.0%
ACAGATTATGCATTAGGCAA+TGG - Chr2:77588394-77588413 MsG0280010953.01.T01:intron 35.0%
ACTAAAGTGGAAAAAAGGAG+AGG + Chr2:77587633-77587652 None:intergenic 35.0%
AGTTGAAGAAAGGAGAATGA+AGG + Chr2:77586902-77586921 None:intergenic 35.0%
ATATTTGTAGACCAACCCAT+TGG - Chr2:77587720-77587739 MsG0280010953.01.T01:intron 35.0%
ATGAAGGTGAAATGAAGAAG+AGG + Chr2:77586886-77586905 None:intergenic 35.0%
ATGTTACAAGATTGGATGCA+AGG - Chr2:77589542-77589561 MsG0280010953.01.T01:CDS 35.0%
CAAGGAAACAAAGCAAAAGA+AGG - Chr2:77588175-77588194 MsG0280010953.01.T01:intron 35.0%
CAATCCTGAAATTCAGTACA+TGG - Chr2:77588366-77588385 MsG0280010953.01.T01:intron 35.0%
CACTTCAGATGTTACAAGAT+TGG - Chr2:77589534-77589553 MsG0280010953.01.T01:CDS 35.0%
CATGTACTGAATTTCAGGAT+TGG + Chr2:77588368-77588387 None:intergenic 35.0%
CGACATTAGAAAGAAATGTG+AGG - Chr2:77588815-77588834 MsG0280010953.01.T01:intron 35.0%
CTAAAGTGGAAAAAAGGAGA+GGG + Chr2:77587632-77587651 None:intergenic 35.0%
CTCTAAATGTGCTTCTCATT+TGG - Chr2:77589132-77589151 MsG0280010953.01.T01:intron 35.0%
CTTGAACATGAAGACAGTAA+AGG - Chr2:77588881-77588900 MsG0280010953.01.T01:CDS 35.0%
CTTTGAATCTCGAAACAGTA+AGG - Chr2:77587450-77587469 MsG0280010953.01.T01:CDS 35.0%
GATCTAATATGCAATTGGCT+TGG - Chr2:77589053-77589072 MsG0280010953.01.T01:intron 35.0%
GATGTATTCATGAGCATAAG+AGG - Chr2:77589305-77589324 MsG0280010953.01.T01:CDS 35.0%
GGTTATTCTGTTGAAGAACT+TGG - Chr2:77587094-77587113 MsG0280010953.01.T01:intron 35.0%
TATCAGATCACAAACATCAC+CGG - Chr2:77588673-77588692 MsG0280010953.01.T01:CDS 35.0%
TATGCCATGTACTGAATTTC+AGG + Chr2:77588373-77588392 None:intergenic 35.0%
TGTTACAAGATTGGATGCAA+GGG - Chr2:77589543-77589562 MsG0280010953.01.T01:CDS 35.0%
! AATAAACAGGGCTTTGCTAT+TGG - Chr2:77588331-77588350 MsG0280010953.01.T01:CDS 35.0%
! ACAATGACAGGCATTTTTCA+AGG - Chr2:77588063-77588082 MsG0280010953.01.T01:CDS 35.0%
! GTCTCCTACAACTTCATTTT+TGG - Chr2:77589213-77589232 MsG0280010953.01.T01:intron 35.0%
! TCCCTTGTTTGGAATGAATA+TGG - Chr2:77587577-77587596 MsG0280010953.01.T01:CDS 35.0%
! TTGTCCTTTGAGAACTGAAA+AGG + Chr2:77587550-77587569 None:intergenic 35.0%
! TTTGGAACAATAGGTGAAAG+AGG - Chr2:77589475-77589494 MsG0280010953.01.T01:CDS 35.0%
!! AACATCTGAAGTGCAACTTT+TGG + Chr2:77589527-77589546 None:intergenic 35.0%
!! AGGCAATGGACTGATTAATA+AGG - Chr2:77588408-77588427 MsG0280010953.01.T01:intron 35.0%
!! ATTTATCTGTTAGGCACTGA+AGG - Chr2:77588604-77588623 MsG0280010953.01.T01:CDS 35.0%
!! CTTCAAATTAAAAGGGTGTC+TGG - Chr2:77589359-77589378 MsG0280010953.01.T01:CDS 35.0%
!! TAAAGGAAGCTTTAGGTGTT+GGG - Chr2:77588898-77588917 MsG0280010953.01.T01:CDS 35.0%
!! TGTTGGTGTTTTGTAGCTAT+TGG - Chr2:77589418-77589437 MsG0280010953.01.T01:CDS 35.0%
!!! CTTCTTTTGCTTTGTTTCCT+TGG + Chr2:77588177-77588196 None:intergenic 35.0%
AACCAGAGTCATCAAAACCA+GGG + Chr2:77587078-77587097 None:intergenic 40.0%
AAGAAATGTGAGGGAGCTTT+CGG - Chr2:77588825-77588844 MsG0280010953.01.T01:CDS 40.0%
AAGAACTTGGTCATCATGCA+GGG - Chr2:77587107-77587126 MsG0280010953.01.T01:intron 40.0%
AATATCACTGTGAACATCGG+TGG + Chr2:77587763-77587782 None:intergenic 40.0%
ACAAGAACTTGTCCCTTGTT+TGG - Chr2:77587566-77587585 MsG0280010953.01.T01:CDS 40.0%
ACAGCATACATTAGTGTTGC+CGG + Chr2:77588695-77588714 None:intergenic 40.0%
AGTCCTCTTATGAGAGTTGA+AGG + Chr2:77586968-77586987 None:intergenic 40.0%
ATAGCTGAAACCTGATCCAA+TGG + Chr2:77587739-77587758 None:intergenic 40.0%
ATCAAGTTACTTGTGTACGC+CGG - Chr2:77589023-77589042 MsG0280010953.01.T01:intron 40.0%
ATTTGGTAGGGAATTCAAGG+TGG - Chr2:77589149-77589168 MsG0280010953.01.T01:intron 40.0%
CAGTTAACCATATGACGACA+GGG + Chr2:77587480-77587499 None:intergenic 40.0%
CGATTTGTATGACTTCTTGC+AGG - Chr2:77587809-77587828 MsG0280010953.01.T01:intron 40.0%
CTCATTTGGTAGGGAATTCA+AGG - Chr2:77589146-77589165 MsG0280010953.01.T01:intron 40.0%
GAATGAATATGGATGGGACA+AGG - Chr2:77587588-77587607 MsG0280010953.01.T01:CDS 40.0%
GCGAATATCACTGTGAACAT+CGG + Chr2:77587766-77587785 None:intergenic 40.0%
GTCAGGTCAGACAACATTTA+AGG - Chr2:77589189-77589208 MsG0280010953.01.T01:intron 40.0%
GTCCTCTTATGAGAGTTGAA+GGG + Chr2:77586967-77586986 None:intergenic 40.0%
TAACCAGAGTCATCAAAACC+AGG + Chr2:77587079-77587098 None:intergenic 40.0%
TAGCTGAAACCTGATCCAAT+GGG + Chr2:77587738-77587757 None:intergenic 40.0%
TCCTCTTATGAGAGTTGAAG+GGG + Chr2:77586966-77586985 None:intergenic 40.0%
TCTTCTCCACAATGTTTCCA+TGG + Chr2:77587045-77587064 None:intergenic 40.0%
TGCCAATACTGTCTTCATCA+CGG + Chr2:77587788-77587807 None:intergenic 40.0%
TTTCATCCAATCTTCCGTCA+AGG + Chr2:77588964-77588983 None:intergenic 40.0%
TTTGGTAGGGAATTCAAGGT+GGG - Chr2:77589150-77589169 MsG0280010953.01.T01:intron 40.0%
TTTGTTTCCTTGGTGAACTC+TGG + Chr2:77588167-77588186 None:intergenic 40.0%
! TGGACCTTTTCAGTTCTCAA+AGG - Chr2:77587543-77587562 MsG0280010953.01.T01:CDS 40.0%
! TTTGGGATGCAGTTGAAGAA+AGG + Chr2:77586912-77586931 None:intergenic 40.0%
!! AAAGGAAGCTTTAGGTGTTG+GGG - Chr2:77588899-77588918 MsG0280010953.01.T01:CDS 40.0%
!! GCAACTTTTGGTTGATCCAT+AGG + Chr2:77589515-77589534 None:intergenic 40.0%
!! GTAAAGGAAGCTTTAGGTGT+TGG - Chr2:77588897-77588916 MsG0280010953.01.T01:CDS 40.0%
!! TTGGTTGATAGTGAAGAAGC+AGG - Chr2:77589232-77589251 MsG0280010953.01.T01:intron 40.0%
AAGGGGATGCAGTTGAAGAA+AGG + Chr2:77586949-77586968 None:intergenic 45.0%
AATGATCCTCATGTCCTCCA+TGG - Chr2:77587025-77587044 MsG0280010953.01.T01:intron 45.0%
AGGACATGAGGATCATTCTC+TGG + Chr2:77587022-77587041 None:intergenic 45.0%
ATATGGTTAACTGGAGGTCC+AGG - Chr2:77587487-77587506 MsG0280010953.01.T01:CDS 45.0%
ATGTTTCCATGGAGGACATG+AGG + Chr2:77587034-77587053 None:intergenic 45.0%
ATTCCATCCTCAAGAAGAGC+AGG + Chr2:77589005-77589024 None:intergenic 45.0%
ATTCCTGCTCTTCTTGAGGA+TGG - Chr2:77588999-77589018 MsG0280010953.01.T01:intron 45.0%
CAAACCTTAAGGAAGGCCAA+AGG + Chr2:77589277-77589296 None:intergenic 45.0%
CCAGTTAACCATATGACGAC+AGG + Chr2:77587481-77587500 None:intergenic 45.0%
CCTGTCGTCATATGGTTAAC+TGG - Chr2:77587478-77587497 MsG0280010953.01.T01:CDS 45.0%
CTAAAGGCGGCAGAAAAATG+TGG - Chr2:77588469-77588488 MsG0280010953.01.T01:intron 45.0%
GAACAATAGGTGAAAGAGGC+TGG - Chr2:77589479-77589498 MsG0280010953.01.T01:CDS 45.0%
GAAGAACTTGGTCATCATGC+AGG - Chr2:77587106-77587125 MsG0280010953.01.T01:intron 45.0%
GAGAAGAAGTTCACTTTCCC+TGG - Chr2:77587058-77587077 MsG0280010953.01.T01:intron 45.0%
GTCGTCATATGGTTAACTGG+AGG - Chr2:77587481-77587500 MsG0280010953.01.T01:CDS 45.0%
GTGTTCGACATATGTGTGTC+TGG - Chr2:77589381-77589400 MsG0280010953.01.T01:CDS 45.0%
TCCCCTTCAACTCTCATAAG+AGG - Chr2:77586962-77586981 MsG0280010953.01.T01:intron 45.0%
TCTCCACAATGTTTCCATGG+AGG + Chr2:77587042-77587061 None:intergenic 45.0%
TCTGAAAAGCCATGGACCTT+TGG - Chr2:77589258-77589277 MsG0280010953.01.T01:intron 45.0%
TGAAACCTGATCCAATGGGT+TGG + Chr2:77587734-77587753 None:intergenic 45.0%
TGAACTCTGGATGCAAAAGC+TGG + Chr2:77588154-77588173 None:intergenic 45.0%
TGTCCTCCATGGAAACATTG+TGG - Chr2:77587036-77587055 MsG0280010953.01.T01:intron 45.0%
TTCCTCATTCCAAAGCTGCT+AGG - Chr2:77587140-77587159 MsG0280010953.01.T01:intron 45.0%
TTGTCAGGACTTCTCTGACA+TGG - Chr2:77588851-77588870 MsG0280010953.01.T01:CDS 45.0%
TTTGCATCCAGAGTTCACCA+AGG - Chr2:77588157-77588176 MsG0280010953.01.T01:intron 45.0%
! GCAGGAATTCTGAAAAGCCA+TGG - Chr2:77589250-77589269 MsG0280010953.01.T01:intron 45.0%
! TTTTCTGCCGCCTTTAGACA+CGG + Chr2:77588466-77588485 None:intergenic 45.0%
!! CAGGGCTTTGCTATTGGTAA+TGG - Chr2:77588337-77588356 MsG0280010953.01.T01:CDS 45.0%
!! TACCTAGCAGCTTTGGAATG+AGG + Chr2:77587145-77587164 None:intergenic 45.0%
!! TATCTGTTAGGCACTGAAGG+TGG - Chr2:77588607-77588626 MsG0280010953.01.T01:CDS 45.0%
!!! TTCCCTGGTTTTGATGACTC+TGG - Chr2:77587073-77587092 MsG0280010953.01.T01:intron 45.0%
ACTGAAGGTGGACAAGCATG+TGG - Chr2:77588619-77588638 MsG0280010953.01.T01:CDS 50.0%
AGGATGACCCTGTCGTCATA+TGG - Chr2:77587470-77587489 MsG0280010953.01.T01:CDS 50.0%
ATGTCCAGCGTATGACTCTC+CGG + Chr2:77588125-77588144 None:intergenic 50.0%
ATTACCGGAGAGTCATACGC+TGG - Chr2:77588118-77588137 MsG0280010953.01.T01:intron 50.0%
CGCCGTGATGAAGACAGTAT+TGG - Chr2:77587783-77587802 MsG0280010953.01.T01:CDS 50.0%
CTGAAGGTGGACAAGCATGT+GGG - Chr2:77588620-77588639 MsG0280010953.01.T01:CDS 50.0%
CTTAGTACACGCTGCCTTGA+CGG - Chr2:77588947-77588966 MsG0280010953.01.T01:CDS 50.0%
GGGTATTCCTGCTCTTCTTG+AGG - Chr2:77588995-77589014 MsG0280010953.01.T01:intron 50.0%
GTAGACCAACCCATTGGATC+AGG - Chr2:77587726-77587745 MsG0280010953.01.T01:intron 50.0%
GTTGATCCCACCGTGTCTAA+AGG - Chr2:77588453-77588472 MsG0280010953.01.T01:intron 50.0%
GTTGATGCCATGAAGTGGTC+AGG - Chr2:77589172-77589191 MsG0280010953.01.T01:intron 50.0%
TAAGGAAGGCCAAAGGTCCA+TGG + Chr2:77589270-77589289 None:intergenic 50.0%
TGGTGTACGTGTGTGTCTGT+TGG - Chr2:77589401-77589420 MsG0280010953.01.T01:CDS 50.0%
TGTCTGACCTGACCACTTCA+TGG + Chr2:77589182-77589201 None:intergenic 50.0%
TTAACTGGAGGTCCAGGATG+CGG - Chr2:77587493-77587512 MsG0280010953.01.T01:CDS 50.0%
! TGGACCTTTGGCCTTCCTTA+AGG - Chr2:77589270-77589289 MsG0280010953.01.T01:intron 50.0%
ACGCTGCCTTGACGGAAGAT+TGG - Chr2:77588955-77588974 MsG0280010953.01.T01:CDS 55.0%
GATCCCACCGTGTCTAAAGG+CGG - Chr2:77588456-77588475 MsG0280010953.01.T01:intron 55.0%
GCAATTTCACTGCCGCATCC+TGG + Chr2:77587508-77587527 None:intergenic 55.0%
GGGAGCTTTCGGTGATTGTC+AGG - Chr2:77588836-77588855 MsG0280010953.01.T01:CDS 55.0%
! GGCTGGTCACATGGTTCCTA+TGG - Chr2:77589496-77589515 MsG0280010953.01.T01:CDS 55.0%
! GGTGAAAGAGGCTGGTCACA+TGG - Chr2:77589487-77589506 MsG0280010953.01.T01:CDS 55.0%
!! GGTGGGTTGATGCCATGAAG+TGG - Chr2:77589167-77589186 MsG0280010953.01.T01:intron 55.0%
CTGCCGCCTTTAGACACGGT+GGG + Chr2:77588462-77588481 None:intergenic 60.0%
TCTGCCGCCTTTAGACACGG+TGG + Chr2:77588463-77588482 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr2 gene 77586824 77589610 77586824 ID=MsG0280010953.01;Name=MsG0280010953.01
Chr2 mRNA 77586824 77589610 77586824 ID=MsG0280010953.01.T01;Parent=MsG0280010953.01;Name=MsG0280010953.01.T01;_AED=0.43;_eAED=0.43;_QI=0|0|0|1|1|1|9|0|513
Chr2 exon 77589273 77589610 77589273 ID=MsG0280010953.01.T01:exon:28001;Parent=MsG0280010953.01.T01
Chr2 exon 77588825 77588999 77588825 ID=MsG0280010953.01.T01:exon:28000;Parent=MsG0280010953.01.T01
Chr2 exon 77588604 77588726 77588604 ID=MsG0280010953.01.T01:exon:27999;Parent=MsG0280010953.01.T01
Chr2 exon 77588218 77588361 77588218 ID=MsG0280010953.01.T01:exon:27998;Parent=MsG0280010953.01.T01
Chr2 exon 77587944 77588094 77587944 ID=MsG0280010953.01.T01:exon:27997;Parent=MsG0280010953.01.T01
Chr2 exon 77587729 77587817 77587729 ID=MsG0280010953.01.T01:exon:27996;Parent=MsG0280010953.01.T01
Chr2 exon 77587360 77587624 77587360 ID=MsG0280010953.01.T01:exon:27995;Parent=MsG0280010953.01.T01
Chr2 exon 77587143 77587276 77587143 ID=MsG0280010953.01.T01:exon:27994;Parent=MsG0280010953.01.T01
Chr2 exon 77586824 77586946 77586824 ID=MsG0280010953.01.T01:exon:27993;Parent=MsG0280010953.01.T01
Chr2 CDS 77589273 77589610 77589273 ID=MsG0280010953.01.T01:cds;Parent=MsG0280010953.01.T01
Chr2 CDS 77588825 77588999 77588825 ID=MsG0280010953.01.T01:cds;Parent=MsG0280010953.01.T01
Chr2 CDS 77588604 77588726 77588604 ID=MsG0280010953.01.T01:cds;Parent=MsG0280010953.01.T01
Chr2 CDS 77588218 77588361 77588218 ID=MsG0280010953.01.T01:cds;Parent=MsG0280010953.01.T01
Chr2 CDS 77587944 77588094 77587944 ID=MsG0280010953.01.T01:cds;Parent=MsG0280010953.01.T01
Chr2 CDS 77587729 77587817 77587729 ID=MsG0280010953.01.T01:cds;Parent=MsG0280010953.01.T01
Chr2 CDS 77587360 77587624 77587360 ID=MsG0280010953.01.T01:cds;Parent=MsG0280010953.01.T01
Chr2 CDS 77587143 77587276 77587143 ID=MsG0280010953.01.T01:cds;Parent=MsG0280010953.01.T01
Chr2 CDS 77586824 77586946 77586824 ID=MsG0280010953.01.T01:cds;Parent=MsG0280010953.01.T01
Gene Sequence

>MsG0280010953.01.T01

ATGGCATCATCTTTAAGGTTCCTCAAAGTGTGTCTGTTTTTTAAAATGTTGTTGCACTTCCTCTTCTTCATTTCACCTTCATTCTCCTTTCTTCAACTGCATCCCAAAAACAATGACATACTCCTTTCTTCAACTGCATCCCCTTCAACTCTCATAAGAGGACTTAACCTTTTTCCTAAAAGTTCCATAAACATACCAGAGAATGATCCTCATGTCCTCCATGGAAACATTGTGGAGAAGAAGTTCACTTTCCCTGGTTTTGATGACTCTGGTTATTCTGTTGAAGAACTTGGTCATCATGCAGGGTATTATAGTCTTCCTCATTCCAAAGCTGCTAGGATGTTCTATTTTTTCTTTGAATCTCGAAACAGTAAGGATGACCCTGTCGTCATATGGTTAACTGGAGGTCCAGGATGCGGCAGTGAAATTGCCTTGTTTTATGAAAATGGACCTTTTCAGTTCTCAAAGGACAAGAACTTGTCCCTTGTTTGGAATGAATATGGATGGGACAAGGCATCAAATATTATATTTGTAGACCAACCCATTGGATCAGGTTTCAGCTATACCACCGATGTTCACAGTGATATTCGCCGTGATGAAGACAGTATTGGCAACGATTTGTATGACTTCTTGCAGGCATTTTTCAAGGAACACCCTCAGTTCACAAAGAATGATTTTTATATTACCGGAGAGTCATACGCTGGACATTACGTTCCAGCTTTTGCATCCAGAGTTCACCAAGGAAACAAAGCAAAAGAAGGAATTCATATAAATCTAAAGGGCTTTGCTATTGGTAATGGATTAACCAATCCTGAAATTCAGTACATGGCATATACAGATTATGCATTAGGCAATGGACTGATTAATAAGGATGAATATGAAAGAATCAACAAGTTGATCCCACCGTGTCTAAAGGCGGCAGAAAAATGTGGCACTGAAGGTGGACAAGCATGTGGGACTGCATTAACTACTTGTATGAAAATATTCTATCAGATCACAAACATCACCGGCAACACTAATTACTACGACATTAGAAAGAAATGTGAGGGAGCTTTCGGTGATTGTCAGGACTTCTCTGACATGGAGACATTCTTGAACATGAAGACAGTAAAGGAAGCTTTAGGTGTTGGGGACTTGAAATTTGATTCTTGCAGTTTCTTAGTACACGCTGCCTTGACGGAAGATTGGATGAAAAATCTTGAAGTGGGTATTCCTGCTCTTCTTGAGGATGGAATCAAGTTACTTGTGTACGCCGGAGAAAAAGATCTAATATGCAATTGGCTTGGGAATTCAAGGTGGGTTGATGCCATGAAGTGGTCAGGTCAGACAACATTTAAGGAGTCTCCTACAACTTCATTTTTGGTTGATAGTGAAGAAGCAGGAATTCTGAAAAGCCATGGACCTTTGGCCTTCCTTAAGGTGAAAGAGGCTGGTCACATGGTTCCTATGGATCAACCAAAAGTTGCACTTCAGATGTTACAAGATTGGATGCAAGGGAAACTGCACTCAGCACAAAACCAAAATCACTTTCTTGTTGTATAA
Protein sequence

>MsG0280010953.01.T01

MASSLRFLKVCLFFKMLLHFLFFISPSFSFLQLHPKNNDILLSSTASPSTLIRGLNLFPKSSINIPENDPHVLHGNIVEKKFTFPGFDDSGYSVEELGHHAGYYSLPHSKAARMFYFFFESRNSKDDPVVIWLTGGPGCGSEIALFYENGPFQFSKDKNLSLVWNEYGWDKASNIIFVDQPIGSGFSYTTDVHSDIRRDEDSIGNDLYDFLQAFFKEHPQFTKNDFYITGESYAGHYVPAFASRVHQGNKAKEGIHINLKGFAIGNGLTNPEIQYMAYTDYALGNGLINKDEYERINKLIPPCLKAAEKCGTEGGQACGTALTTCMKIFYQITNITGNTNYYDIRKKCEGAFGDCQDFSDMETFLNMKTVKEALGVGDLKFDSCSFLVHAALTEDWMKNLEVGIPALLEDGIKLLVYAGEKDLICNWLGNSRWVDAMKWSGQTTFKESPTTSFLVDSEEAGILKSHGPLAFLKVKEAGHMVPMDQPKVALQMLQDWMQGKLHSAQNQNHFLVV*