AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280011019.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280011019.01.T01 MTR_0053s0020 83.284 341 9 3 1 333 1 301 1.83e-178 496
MsG0280011019.01.T01 MTR_0092s0090 80.162 247 9 2 1 247 1 207 1.56e-112 330
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280011019.01.T01 AT5G51170 59.746 236 72 3 105 332 64 284 1.04e-92 278
MsG0280011019.01.T01 AT5G51170 54.661 236 56 3 105 332 64 256 1.72e-82 251
MsG0280011019.01.T01 AT5G51170 63.077 195 63 2 146 332 28 221 7.01e-82 248
MsG0280011019.01.T01 AT5G51170 60.140 143 42 2 105 247 64 191 5.84e-52 172

Find 70 sgRNAs with CRISPR-Local

Find 272 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTGCTTCTTTATCCTTAT+TGG 0.126930 2:+78472345 MsG0280011019.01.T01:three_prime_UTR
CGGATCTGCCAAAATATTTA+TGG 0.159427 2:+78472261 MsG0280011019.01.T01:three_prime_UTR
GGATGCAACCATAAATATTT+TGG 0.187256 2:-78472269 None:intergenic
GAAATCAAGAAATGTGTTTC+TGG 0.204852 2:+78472114 MsG0280011019.01.T01:CDS
TTCGATACCTTTGAATTTAC+AGG 0.211499 2:-78472163 None:intergenic
CCCAGCATACCAAGAAAATT+TGG 0.234445 2:-78467936 None:intergenic
CTTCTTTATCCTTATTGGTT+TGG 0.281203 2:+78472350 MsG0280011019.01.T01:three_prime_UTR
AAGGGAGTTTCACATAAGTT+TGG 0.299639 2:+78469715 MsG0280011019.01.T01:CDS
AGCTCCTAACTTTAGTTGTC+TGG 0.332076 2:-78468324 None:intergenic
TAGGAGGTGGCGGCAAGGGC+TGG 0.366489 2:-78467896 None:intergenic
TACAATGTACCAAACCAATA+AGG 0.366947 2:-78472359 None:intergenic
GAGGCATTAATGCAATGTTA+TGG 0.379241 2:+78467747 MsG0280011019.01.T01:CDS
ACACTGACCTCGTAAAATTC+AGG 0.393841 2:-78471708 None:intergenic
GTTTGTTGCCCGGGGTGGCA+AGG 0.397405 2:-78467844 None:intergenic
GCTTTAAGGTGCAGTGACTT+TGG 0.398713 2:+78469517 MsG0280011019.01.T01:intron
AGGGAGTTTCACATAAGTTT+GGG 0.402709 2:+78469716 MsG0280011019.01.T01:CDS
CATTTCGTTAGCATGGTCAT+TGG 0.406486 2:+78472053 MsG0280011019.01.T01:CDS
AGGAGCTTCCCTCATGTTGA+TGG 0.416207 2:+78468340 MsG0280011019.01.T01:CDS
TAAGCCAGACAACTAAAGTT+AGG 0.418446 2:+78468320 MsG0280011019.01.T01:CDS
GGAGGATGAAGGAGCGCAAT+AGG 0.426335 2:-78467915 None:intergenic
AGGCATTAATGCAATGTTAT+GGG 0.426486 2:+78467748 MsG0280011019.01.T01:CDS
TTCACGAATGTTTGTTGCCC+GGG 0.435513 2:-78467853 None:intergenic
AAATCAAGAAATGTGTTTCT+GGG 0.446858 2:+78472115 MsG0280011019.01.T01:CDS
TCAATCTGATGTACTCGTAT+TGG 0.447949 2:-78469749 None:intergenic
CAGTCTACATAGAATAGAAC+TGG 0.451596 2:-78472290 None:intergenic
TGCATAAGCTAAGGTTGGAT+TGG 0.452098 2:-78467808 None:intergenic
ATTTCGTTAGCATGGTCATT+GGG 0.452771 2:+78472054 MsG0280011019.01.T01:CDS
CTTTCTGCATAAGCTAAGGT+TGG 0.456737 2:-78467813 None:intergenic
AAAGGTATCGAATGTAAGAT+TGG 0.468840 2:+78472174 MsG0280011019.01.T01:CDS
ACTGGATCGACTTTGACAAA+TGG 0.473405 2:+78469912 MsG0280011019.01.T01:CDS
ACTTGGTATGTATACATGTA+AGG 0.484192 2:-78468371 None:intergenic
TTTGTTGCCCGGGGTGGCAA+GGG 0.491040 2:-78467843 None:intergenic
AATGAACATTCATCATCAAA+AGG 0.492995 2:-78468267 None:intergenic
AATGTTCATTGTTGTGATGA+AGG 0.496386 2:+78468280 MsG0280011019.01.T01:CDS
GTTCACGAATGTTTGTTGCC+CGG 0.496813 2:-78467854 None:intergenic
CGCAATAGGAGGTGGCGGCA+AGG 0.500205 2:-78467901 None:intergenic
CAAGAAAATTTGGAGGATGA+AGG 0.508763 2:-78467926 None:intergenic
CTGGATCGACTTTGACAAAT+GGG 0.509720 2:+78469913 MsG0280011019.01.T01:CDS
GCAATAGGAGGTGGCGGCAA+GGG 0.510215 2:-78467900 None:intergenic
CAGATAGATGATAATGTGCA+TGG 0.512307 2:+78472226 MsG0280011019.01.T01:exon
ACATCAACGGCACAAAGACT+TGG 0.514481 2:-78469632 None:intergenic
ACGTGAGCCCTTGCCACCCC+GGG 0.528418 2:+78467836 MsG0280011019.01.T01:CDS
CGAATGTTTGTTGCCCGGGG+TGG 0.532635 2:-78467849 None:intergenic
GACGTGAGCCCTTGCCACCC+CGG 0.539443 2:+78467835 MsG0280011019.01.T01:CDS
GTCCTCACATTTCGTTAGCA+TGG 0.540870 2:+78472046 MsG0280011019.01.T01:CDS
TAAGCTAAGGTTGGATTGGA+AGG 0.544475 2:-78467804 None:intergenic
TGGACGCATGCTTTGTGAAG+TGG 0.546759 2:-78467876 None:intergenic
ACGTCTTTCTGCATAAGCTA+AGG 0.555280 2:-78467817 None:intergenic
TGAAGGAGCGCAATAGGAGG+TGG 0.555464 2:-78467909 None:intergenic
AACAACCTCGACTGAGAGGA+AGG 0.577707 2:-78469966 None:intergenic
TTAATGCAATGTTATGGGGA+TGG 0.578101 2:+78467753 MsG0280011019.01.T01:CDS
TGGCTTATCTGAATATCTTG+AGG 0.583122 2:-78468304 None:intergenic
AGGAGCGCAATAGGAGGTGG+CGG 0.592012 2:-78467906 None:intergenic
GCTAACGAAATGTGAGGACG+TGG 0.594436 2:-78472042 None:intergenic
GTGCATGGTTTATAGAGTAA+CGG 0.596404 2:+78472241 MsG0280011019.01.T01:three_prime_UTR
AGCATACCAAGAAAATTTGG+AGG 0.602667 2:-78467933 None:intergenic
ATGTTCATTGTTGTGATGAA+GGG 0.610785 2:+78468281 MsG0280011019.01.T01:CDS
GCATAGTTACCATCAACATG+AGG 0.627181 2:-78468349 None:intergenic
GGATGAAGGAGCGCAATAGG+AGG 0.627367 2:-78467912 None:intergenic
GGCATTAATGCAATGTTATG+GGG 0.632606 2:+78467749 MsG0280011019.01.T01:CDS
CATAGTTACCATCAACATGA+GGG 0.639206 2:-78468348 None:intergenic
ACGCACCTTCCTCTCAGTCG+AGG 0.647582 2:+78469961 MsG0280011019.01.T01:CDS
TCACGAATGTTTGTTGCCCG+GGG 0.650250 2:-78467852 None:intergenic
AAGTGGGACATCAACATCAA+CGG 0.651923 2:-78469645 None:intergenic
GATCGACTTTGACAAATGGG+AGG 0.652184 2:+78469916 MsG0280011019.01.T01:CDS
CGTGAACAACCTCGACTGAG+AGG 0.653411 2:-78469970 None:intergenic
CGATTCCTTGAAGAAAACTG+TGG 0.667788 2:+78472086 MsG0280011019.01.T01:CDS
TGACCTCGTAAAATTCAGGA+AGG 0.669807 2:-78471704 None:intergenic
GACCATGCTAACGAAATGTG+AGG 0.681537 2:-78472048 None:intergenic
AGTACATCAGATTGACTCAG+TGG 0.704214 2:+78469757 MsG0280011019.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAGCAAAAAAATGTT+CGG + Chr2:78471911-78471930 MsG0280011019.01.T01:intron 15.0%
!! AAAACAATAATTGAAAAACT+CGG + Chr2:78468986-78469005 MsG0280011019.01.T01:intron 15.0%
!! ATTACATGTAAATCTTAAAA+AGG - Chr2:78469304-78469323 None:intergenic 15.0%
!!! AATAATTGTTTTGAAATGTT+TGG + Chr2:78468795-78468814 MsG0280011019.01.T01:intron 15.0%
!!! ATAATTGTTTTGAAATGTTT+GGG + Chr2:78468796-78468815 MsG0280011019.01.T01:intron 15.0%
!! AAAAGGCAAAATATGAATAT+TGG - Chr2:78470136-78470155 None:intergenic 20.0%
!! AAACAAGAATAAAAACTTAC+AGG - Chr2:78469817-78469836 None:intergenic 20.0%
!! AACAAGAATAAAAACTTACA+GGG - Chr2:78469816-78469835 None:intergenic 20.0%
!! AATGATGATAGAAGATATTA+AGG - Chr2:78471977-78471996 None:intergenic 20.0%
!! ACAATACTACTTAATCTTAA+AGG - Chr2:78471305-78471324 None:intergenic 20.0%
!! ACAATTATTAATCAAGTTTG+TGG - Chr2:78468784-78468803 None:intergenic 20.0%
!! CTGATATATAATATTGTTGA+TGG + Chr2:78470169-78470188 MsG0280011019.01.T01:intron 20.0%
!! GCAATTATGAAATAAATGTT+GGG - Chr2:78471255-78471274 None:intergenic 20.0%
!! TAAATAAAATGCAAGTTTAG+CGG - Chr2:78471465-78471484 None:intergenic 20.0%
!! TAAGATAATGTTAACTTACT+TGG - Chr2:78468391-78468410 None:intergenic 20.0%
!! TAATTAAAATATGTTCCTTG+TGG + Chr2:78467998-78468017 MsG0280011019.01.T01:intron 20.0%
!! TACTAACACAATAAATATAG+AGG - Chr2:78470045-78470064 None:intergenic 20.0%
!! TATATGATGATATAAGCTTT+AGG - Chr2:78471338-78471357 None:intergenic 20.0%
!! TGCAATTATGAAATAAATGT+TGG - Chr2:78471256-78471275 None:intergenic 20.0%
!!! AAATATAGAGGCATAAAAAT+TGG - Chr2:78470033-78470052 None:intergenic 20.0%
!!! AATGAACTCTTATTTATATG+TGG + Chr2:78468225-78468244 MsG0280011019.01.T01:intron 20.0%
!!! ATTTTTACCTGTAAATTCAA+AGG + Chr2:78472156-78472175 MsG0280011019.01.T01:CDS 20.0%
!!! CATTTTATTTGATGAATCTA+AGG + Chr2:78471367-78471386 MsG0280011019.01.T01:intron 20.0%
!!! TATATTTAAGACAAATGTGA+TGG - Chr2:78468548-78468567 None:intergenic 20.0%
!!! TCTTGTTTTGAAAAATTGAA+CGG + Chr2:78469829-78469848 MsG0280011019.01.T01:intron 20.0%
!!! TTAAAGCTAATTGATTCAAA+AGG + Chr2:78469352-78469371 MsG0280011019.01.T01:intron 20.0%
!!! TTTTCAATTGATTTCAGATA+AGG + Chr2:78471640-78471659 MsG0280011019.01.T01:intron 20.0%
! AAAGAAACACACATTTAATG+TGG - Chr2:78471623-78471642 None:intergenic 25.0%
! AAATAAAATGCAAGTTTAGC+GGG - Chr2:78471464-78471483 None:intergenic 25.0%
! AAATCAAGAAATGTGTTTCT+GGG + Chr2:78472115-78472134 MsG0280011019.01.T01:CDS 25.0%
! AAATCTGTTACTATATGCTT+TGG + Chr2:78470823-78470842 MsG0280011019.01.T01:intron 25.0%
! AATGAACATTCATCATCAAA+AGG - Chr2:78468270-78468289 None:intergenic 25.0%
! ACAAGAATAAAAACTTACAG+GGG - Chr2:78469815-78469834 None:intergenic 25.0%
! AGTTAACATTATCTTAGTGT+TGG + Chr2:78468395-78468414 MsG0280011019.01.T01:intron 25.0%
! ATAGAGATATTCAGAATATG+TGG + Chr2:78470907-78470926 MsG0280011019.01.T01:intron 25.0%
! ATTACCCGATACTTATAATA+AGG - Chr2:78470631-78470650 None:intergenic 25.0%
! ATTATTTATTTGATTGCCGA+TGG - Chr2:78468208-78468227 None:intergenic 25.0%
! ATTCATCAAATAAAATGACG+TGG - Chr2:78471364-78471383 None:intergenic 25.0%
! TAATATTCTCATCTTTGCTA+TGG + Chr2:78471199-78471218 MsG0280011019.01.T01:intron 25.0%
! TATAGTAACAGATTTCATCT+TGG - Chr2:78470818-78470837 None:intergenic 25.0%
! TCAAGCATTATAAAGTAACA+TGG - Chr2:78469457-78469476 None:intergenic 25.0%
! TCAGCAGTTCTATTAAAAAA+AGG - Chr2:78470153-78470172 None:intergenic 25.0%
! TGAAAATCAGCAGTTTATTA+GGG + Chr2:78471782-78471801 MsG0280011019.01.T01:intron 25.0%
! TTCACTTAGAGAATACATTA+TGG + Chr2:78471838-78471857 MsG0280011019.01.T01:intron 25.0%
! TTCTATTGCAAATGAAGTTT+CGG + Chr2:78471144-78471163 MsG0280011019.01.T01:intron 25.0%
! TTGAAAATCAGCAGTTTATT+AGG + Chr2:78471781-78471800 MsG0280011019.01.T01:intron 25.0%
! TTTAATCATCATTTGTAACC+AGG + Chr2:78468648-78468667 MsG0280011019.01.T01:intron 25.0%
!! GATTTTCTTACTCTTTACAA+GGG + Chr2:78467968-78467987 MsG0280011019.01.T01:CDS 25.0%
!! TCATCATTCTGAAAAATATG+AGG - Chr2:78468082-78468101 None:intergenic 25.0%
!! TGATATTTTCTTCAAGAAAG+CGG - Chr2:78469603-78469622 None:intergenic 25.0%
!! TGATTTTCTTACTCTTTACA+AGG + Chr2:78467967-78467986 MsG0280011019.01.T01:CDS 25.0%
!!! ATTGTTGATGGTAAAGTAAT+AGG + Chr2:78470181-78470200 MsG0280011019.01.T01:intron 25.0%
!!! TTGTTGATGGTAAAGTAATA+GGG + Chr2:78470182-78470201 MsG0280011019.01.T01:intron 25.0%
AAAAAACCAACATTGAACCT+TGG - Chr2:78470786-78470805 None:intergenic 30.0%
AAAATATGTTCCTTGTGGAT+AGG + Chr2:78468003-78468022 MsG0280011019.01.T01:intron 30.0%
AAATAAATGTTGGGCAGTAA+AGG - Chr2:78471246-78471265 None:intergenic 30.0%
AAATTCTAAACCTATCCACA+AGG - Chr2:78468016-78468035 None:intergenic 30.0%
AATAAATGTTGGGCAGTAAA+GGG - Chr2:78471245-78471264 None:intergenic 30.0%
AATAACACCAGAATTATACC+AGG - Chr2:78471808-78471827 None:intergenic 30.0%
AATGTTCATTGTTGTGATGA+AGG + Chr2:78468280-78468299 MsG0280011019.01.T01:CDS 30.0%
AATTAACCAAGGTTCAATGT+TGG + Chr2:78470777-78470796 MsG0280011019.01.T01:intron 30.0%
ACTTGCTTCTTTATCCTTAT+TGG + Chr2:78472345-78472364 MsG0280011019.01.T01:three_prime_UTR 30.0%
ACTTGGTATGTATACATGTA+AGG - Chr2:78468374-78468393 None:intergenic 30.0%
AGAGAGATATAAACAAGTCT+TGG - Chr2:78471522-78471541 None:intergenic 30.0%
AGGCATTAATGCAATGTTAT+GGG + Chr2:78467748-78467767 MsG0280011019.01.T01:CDS 30.0%
AGTTCGTCAGTAAGAATAAA+AGG + Chr2:78470296-78470315 MsG0280011019.01.T01:intron 30.0%
ATGAAGAATCAAGAACACTT+AGG - Chr2:78470557-78470576 None:intergenic 30.0%
ATGTTCATTGTTGTGATGAA+GGG + Chr2:78468281-78468300 MsG0280011019.01.T01:CDS 30.0%
CAGTCCTTATTATAAGTATC+GGG + Chr2:78470624-78470643 MsG0280011019.01.T01:intron 30.0%
CCAGTCCTTATTATAAGTAT+CGG + Chr2:78470623-78470642 MsG0280011019.01.T01:intron 30.0%
GAAATCAAGAAATGTGTTTC+TGG + Chr2:78472114-78472133 MsG0280011019.01.T01:CDS 30.0%
GGTAATTGAAATCGGATTAT+TGG + Chr2:78470645-78470664 MsG0280011019.01.T01:intron 30.0%
GTTCGTCAGTAAGAATAAAA+GGG + Chr2:78470297-78470316 MsG0280011019.01.T01:intron 30.0%
TAACTTCAAAGAACAATTGC+AGG - Chr2:78468694-78468713 None:intergenic 30.0%
TAATTCATCATTAGTGTTCC+AGG + Chr2:78470745-78470764 MsG0280011019.01.T01:intron 30.0%
TACTTATAATAAGGACTGGA+CGG - Chr2:78470622-78470641 None:intergenic 30.0%
TCTATCATAGGATTTAACTC+AGG - Chr2:78470958-78470977 None:intergenic 30.0%
TGAAATCGGATTATTGGTAA+TGG + Chr2:78470651-78470670 MsG0280011019.01.T01:intron 30.0%
TGAAGTTATACTTTCCTGTT+TGG + Chr2:78468706-78468725 MsG0280011019.01.T01:intron 30.0%
TGCTCAAAATGACAAGTTAA+AGG - Chr2:78469889-78469908 None:intergenic 30.0%
TTCGATACCTTTGAATTTAC+AGG - Chr2:78472166-78472185 None:intergenic 30.0%
! AAGCTTGAACAACTTTTACT+AGG + Chr2:78469692-78469711 MsG0280011019.01.T01:CDS 30.0%
! AATCATTTAAAGCAATACCC+TGG + Chr2:78469090-78469109 MsG0280011019.01.T01:intron 30.0%
! AGATGATAGAATTTTGTGCA+AGG - Chr2:78471123-78471142 None:intergenic 30.0%
! AGTCAAATTTCAAAGTTGGT+TGG + Chr2:78469113-78469132 MsG0280011019.01.T01:intron 30.0%
! ATAAAGTAACATGGAAGTAC+AGG - Chr2:78469448-78469467 None:intergenic 30.0%
! ATGTTTGAAGTTCTTTCCAT+CGG + Chr2:78468189-78468208 MsG0280011019.01.T01:intron 30.0%
! CTAAGCATTGCATATGATTA+TGG + Chr2:78468494-78468513 MsG0280011019.01.T01:intron 30.0%
! CTTCTTTATCCTTATTGGTT+TGG + Chr2:78472350-78472369 MsG0280011019.01.T01:three_prime_UTR 30.0%
! GGAATTTAGAGTTAGTTAGT+TGG + Chr2:78469021-78469040 MsG0280011019.01.T01:intron 30.0%
! GGATGCAACCATAAATATTT+TGG - Chr2:78472272-78472291 None:intergenic 30.0%
! GTCAAATTTCAAAGTTGGTT+GGG + Chr2:78469114-78469133 MsG0280011019.01.T01:intron 30.0%
! TCAAATTTCAAAGTTGGTTG+GGG + Chr2:78469115-78469134 MsG0280011019.01.T01:intron 30.0%
! TCATCATTCTGAAAAATACG+AGG - Chr2:78468131-78468150 None:intergenic 30.0%
! TGAACAACTTTTACTAGGAA+GGG + Chr2:78469697-78469716 MsG0280011019.01.T01:CDS 30.0%
! TGTGGATAGGTTTAGAATTT+AGG + Chr2:78468016-78468035 MsG0280011019.01.T01:intron 30.0%
! TTAAATAGTCTTGCAAACAC+TGG - Chr2:78469411-78469430 None:intergenic 30.0%
! TTGAACAACTTTTACTAGGA+AGG + Chr2:78469696-78469715 MsG0280011019.01.T01:CDS 30.0%
! TTGCATGTTTTATAGTTGTG+TGG + Chr2:78471271-78471290 MsG0280011019.01.T01:intron 30.0%
! TTTTCAAAACTTAGTGACCA+AGG - Chr2:78468854-78468873 None:intergenic 30.0%
! TTTTCTGTTTTGTCATACAG+TGG + Chr2:78468425-78468444 MsG0280011019.01.T01:intron 30.0%
!! AAAGGTATCGAATGTAAGAT+TGG + Chr2:78472174-78472193 MsG0280011019.01.T01:CDS 30.0%
!! AACTTTGAAATTTGACTCCA+GGG - Chr2:78469110-78469129 None:intergenic 30.0%
!! AAGTATCGGGTAATTGAAAT+CGG + Chr2:78470637-78470656 MsG0280011019.01.T01:intron 30.0%
!! AGAAATTTTGATGCAAGACT+TGG - Chr2:78468881-78468900 None:intergenic 30.0%
!! GCTGATTTTCAAAAACAGTT+TGG - Chr2:78471773-78471792 None:intergenic 30.0%
!! TATATGCTTTGGAATTTCGA+GGG + Chr2:78470834-78470853 MsG0280011019.01.T01:intron 30.0%
!! TGTTGATGGTAAAGTAATAG+GGG + Chr2:78470183-78470202 MsG0280011019.01.T01:intron 30.0%
AAAGGCAACAATCAGGTAAA+CGG - Chr2:78469871-78469890 None:intergenic 35.0%
AAATGTGATGGTTAATTCCC+AGG - Chr2:78468536-78468555 None:intergenic 35.0%
AAGGGAGTTTCACATAAGTT+TGG + Chr2:78469715-78469734 MsG0280011019.01.T01:CDS 35.0%
AAGTTTGGAGAAGTTGTGTT+TGG + Chr2:78470682-78470701 MsG0280011019.01.T01:intron 35.0%
ACAAGTTAAAGGCAACAATC+AGG - Chr2:78469878-78469897 None:intergenic 35.0%
ACCTAGAAAGAATGCATTTC+CGG - Chr2:78471942-78471961 None:intergenic 35.0%
AGACTATAGAGTCAGTTCAA+AGG - Chr2:78470981-78471000 None:intergenic 35.0%
AGCATACCAAGAAAATTTGG+AGG - Chr2:78467936-78467955 None:intergenic 35.0%
AGGGAGTTTCACATAAGTTT+GGG + Chr2:78469716-78469735 MsG0280011019.01.T01:CDS 35.0%
AGGTTAAGGTGATCTCAAAT+TGG + Chr2:78468668-78468687 MsG0280011019.01.T01:intron 35.0%
AGTAACAGATTTCATCTTGG+AGG - Chr2:78470815-78470834 None:intergenic 35.0%
AGTAGTAATAGGAAGGAAAC+AGG + Chr2:78470237-78470256 MsG0280011019.01.T01:intron 35.0%
ATAGGATTTAACTCAGGCTA+GGG - Chr2:78470952-78470971 None:intergenic 35.0%
ATATGCCAAACTATTAGCAG+AGG - Chr2:78471007-78471026 None:intergenic 35.0%
ATTCTTTGCAAGTGTCTGTA+AGG - Chr2:78470881-78470900 None:intergenic 35.0%
ATTTCGTTAGCATGGTCATT+GGG + Chr2:78472054-78472073 MsG0280011019.01.T01:CDS 35.0%
CAAGAAAATTTGGAGGATGA+AGG - Chr2:78467929-78467948 None:intergenic 35.0%
CAGTCTACATAGAATAGAAC+TGG - Chr2:78472293-78472312 None:intergenic 35.0%
CATAGTTACCATCAACATGA+GGG - Chr2:78468351-78468370 None:intergenic 35.0%
CATCATTTGTAACCAGGTTA+AGG + Chr2:78468654-78468673 MsG0280011019.01.T01:intron 35.0%
CCGATACTTATAATAAGGAC+TGG - Chr2:78470626-78470645 None:intergenic 35.0%
CGGATCTGCCAAAATATTTA+TGG + Chr2:78472261-78472280 MsG0280011019.01.T01:three_prime_UTR 35.0%
CTATACCTCTGCTAATAGTT+TGG + Chr2:78470999-78471018 MsG0280011019.01.T01:intron 35.0%
CTGGAGTCAAATTTCAAAGT+TGG + Chr2:78469109-78469128 MsG0280011019.01.T01:intron 35.0%
CTTGAGGAATCAATTGAGAT+AGG - Chr2:78469188-78469207 None:intergenic 35.0%
GAAACACAAGACTTGTGATA+TGG + Chr2:78471395-78471414 MsG0280011019.01.T01:intron 35.0%
GCATGCATTTAGATTTACCT+GGG + Chr2:78468516-78468535 MsG0280011019.01.T01:intron 35.0%
GGACAACATTGAATTAACCA+AGG + Chr2:78470766-78470785 MsG0280011019.01.T01:intron 35.0%
GGCATTAATGCAATGTTATG+GGG + Chr2:78467749-78467768 MsG0280011019.01.T01:CDS 35.0%
GTAGTAATAGGAAGGAAACA+GGG + Chr2:78470238-78470257 MsG0280011019.01.T01:intron 35.0%
GTATGGTAGTAGTAATAGGA+AGG + Chr2:78470230-78470249 MsG0280011019.01.T01:intron 35.0%
GTCAGTAAGAATAAAAGGGT+GGG + Chr2:78470301-78470320 MsG0280011019.01.T01:intron 35.0%
GTGCATGGTTTATAGAGTAA+CGG + Chr2:78472241-78472260 MsG0280011019.01.T01:three_prime_UTR 35.0%
GTGTTAGTACATATGCAAGT+TGG + Chr2:78470056-78470075 MsG0280011019.01.T01:intron 35.0%
GTTAGTTGGTTAGAGTTAGT+TGG + Chr2:78469035-78469054 MsG0280011019.01.T01:intron 35.0%
TAACGGAATCAGAATCAAAC+AGG + Chr2:78470495-78470514 MsG0280011019.01.T01:intron 35.0%
TAAGCCAGACAACTAAAGTT+AGG + Chr2:78468320-78468339 MsG0280011019.01.T01:CDS 35.0%
TACAGACACTTGCAAAGAAT+AGG + Chr2:78470881-78470900 MsG0280011019.01.T01:intron 35.0%
TAGTAATAGGAAGGAAACAG+GGG + Chr2:78470239-78470258 MsG0280011019.01.T01:intron 35.0%
TCAATCTGATGTACTCGTAT+TGG - Chr2:78469752-78469771 None:intergenic 35.0%
TCAGTAAGAATAAAAGGGTG+GGG + Chr2:78470302-78470321 MsG0280011019.01.T01:intron 35.0%
TCAGTTCAAAGGTCTATCAT+AGG - Chr2:78470970-78470989 None:intergenic 35.0%
TGGCTTATCTGAATATCTTG+AGG - Chr2:78468307-78468326 None:intergenic 35.0%
TGGTTAATTCAATGTTGTCC+TGG - Chr2:78470766-78470785 None:intergenic 35.0%
TGTTGCAGAGAAAGAAAGAA+TGG - Chr2:78468622-78468641 None:intergenic 35.0%
TTAATGCAATGTTATGGGGA+TGG + Chr2:78467753-78467772 MsG0280011019.01.T01:CDS 35.0%
! AAACCTGAAGTATTCTGTAC+AGG + Chr2:78472017-78472036 MsG0280011019.01.T01:intron 35.0%
! AATTTGAGATCACCTTAACC+TGG - Chr2:78468669-78468688 None:intergenic 35.0%
! CACAGTTTTCTTCAAGGAAT+CGG - Chr2:78472088-78472107 None:intergenic 35.0%
! CAGATAGATGATAATGTGCA+TGG + Chr2:78472226-78472245 MsG0280011019.01.T01:exon 35.0%
! CCAAATTTTCTTGGTATGCT+GGG + Chr2:78467936-78467955 MsG0280011019.01.T01:CDS 35.0%
! CTTCATCCTCCAAATTTTCT+TGG + Chr2:78467927-78467946 MsG0280011019.01.T01:CDS 35.0%
! GAGGCATTAATGCAATGTTA+TGG + Chr2:78467747-78467766 MsG0280011019.01.T01:CDS 35.0%
! GTAATGGAATGTGAGAAGTT+TGG + Chr2:78470667-78470686 MsG0280011019.01.T01:intron 35.0%
! GTCTTGTAGATCTGTTTCTT+GGG + Chr2:78471038-78471057 MsG0280011019.01.T01:intron 35.0%
! GTTTGGCATATAGTTTTCCT+TGG + Chr2:78471016-78471035 MsG0280011019.01.T01:intron 35.0%
! GTTTTGTCATACAGTGGTTA+TGG + Chr2:78468431-78468450 MsG0280011019.01.T01:intron 35.0%
! TAACCTTCCTGAATTTTACG+AGG + Chr2:78471701-78471720 MsG0280011019.01.T01:CDS 35.0%
! TATGCATTTCTAGATCCTGT+AGG - Chr2:78469252-78469271 None:intergenic 35.0%
! TCCAAATTTTCTTGGTATGC+TGG + Chr2:78467935-78467954 MsG0280011019.01.T01:CDS 35.0%
! TTCAAAGTGTTCCTGCTTTA+AGG + Chr2:78469503-78469522 MsG0280011019.01.T01:intron 35.0%
! TTTTACAGAGGACGTTAAGT+GGG - Chr2:78469664-78469683 None:intergenic 35.0%
! TTTTTACAGAGGACGTTAAG+TGG - Chr2:78469665-78469684 None:intergenic 35.0%
!! AAAAAAATGTTCGGTGCTTC+CGG + Chr2:78471920-78471939 MsG0280011019.01.T01:intron 35.0%
!! CAACTTTGAAATTTGACTCC+AGG - Chr2:78469111-78469130 None:intergenic 35.0%
!! CTATATGCTTTGGAATTTCG+AGG + Chr2:78470833-78470852 MsG0280011019.01.T01:intron 35.0%
!! GTTGATGGTAAAGTAATAGG+GGG + Chr2:78470184-78470203 MsG0280011019.01.T01:intron 35.0%
!!! TGTTTTGATCCACATTTGTG+AGG + Chr2:78468741-78468760 MsG0280011019.01.T01:intron 35.0%
!!! TTGTCATTTTGAGCAGATAC+TGG + Chr2:78469894-78469913 MsG0280011019.01.T01:intron 35.0%
AAAACTCGGACTGTTAGTTG+AGG + Chr2:78469000-78469019 MsG0280011019.01.T01:intron 40.0%
AAGGCAACAATCAGGTAAAC+GGG - Chr2:78469870-78469889 None:intergenic 40.0%
AAGTGGGACATCAACATCAA+CGG - Chr2:78469648-78469667 None:intergenic 40.0%
ACACTGACCTCGTAAAATTC+AGG - Chr2:78471711-78471730 None:intergenic 40.0%
ACCTATTTGCCTCACAAATG+TGG - Chr2:78468753-78468772 None:intergenic 40.0%
ACGTCTTTCTGCATAAGCTA+AGG - Chr2:78467820-78467839 None:intergenic 40.0%
AGCTCCTAACTTTAGTTGTC+TGG - Chr2:78468327-78468346 None:intergenic 40.0%
ATTAGGGCCTGGTATAATTC+TGG + Chr2:78471798-78471817 MsG0280011019.01.T01:intron 40.0%
CAAACAAGGGTTAGACATGA+CGG - Chr2:78471071-78471090 None:intergenic 40.0%
CATAGGATTTAACTCAGGCT+AGG - Chr2:78470953-78470972 None:intergenic 40.0%
CATGCAATAACAGTCCAAAC+AGG - Chr2:78468723-78468742 None:intergenic 40.0%
CATTTCGTTAGCATGGTCAT+TGG + Chr2:78472053-78472072 MsG0280011019.01.T01:CDS 40.0%
CCCAGCATACCAAGAAAATT+TGG - Chr2:78467939-78467958 None:intergenic 40.0%
CGATTCCTTGAAGAAAACTG+TGG + Chr2:78472086-78472105 MsG0280011019.01.T01:CDS 40.0%
CGTCAGTAAGAATAAAAGGG+TGG + Chr2:78470300-78470319 MsG0280011019.01.T01:intron 40.0%
CTTACCATCTCTAACAGTCT+CGG - Chr2:78469063-78469082 None:intergenic 40.0%
CTTTCTGCATAAGCTAAGGT+TGG - Chr2:78467816-78467835 None:intergenic 40.0%
GAAACAGATCTACAAGACCA+AGG - Chr2:78471036-78471055 None:intergenic 40.0%
GAGCGTATGGTAGTAGTAAT+AGG + Chr2:78470226-78470245 MsG0280011019.01.T01:intron 40.0%
GATCCTGTACAGAATACTTC+AGG - Chr2:78472023-78472042 None:intergenic 40.0%
GCATAGTTACCATCAACATG+AGG - Chr2:78468352-78468371 None:intergenic 40.0%
GGCATGCATTTAGATTTACC+TGG + Chr2:78468515-78468534 MsG0280011019.01.T01:intron 40.0%
TCCACATTTGTGAGGCAAAT+AGG + Chr2:78468749-78468768 MsG0280011019.01.T01:intron 40.0%
TCCGGAAATGCATTCTTTCT+AGG + Chr2:78471938-78471957 MsG0280011019.01.T01:intron 40.0%
TGACCTCGTAAAATTCAGGA+AGG - Chr2:78471707-78471726 None:intergenic 40.0%
TGCATAAGCTAAGGTTGGAT+TGG - Chr2:78467811-78467830 None:intergenic 40.0%
TTGTGTTTGGAAGCTGAACA+TGG + Chr2:78470695-78470714 MsG0280011019.01.T01:intron 40.0%
! ACTGGATCGACTTTGACAAA+TGG + Chr2:78469912-78469931 MsG0280011019.01.T01:CDS 40.0%
! AGTACATCAGATTGACTCAG+TGG + Chr2:78469757-78469776 MsG0280011019.01.T01:CDS 40.0%
! CTGGATCGACTTTGACAAAT+GGG + Chr2:78469913-78469932 MsG0280011019.01.T01:CDS 40.0%
! GGTCTTGTAGATCTGTTTCT+TGG + Chr2:78471037-78471056 MsG0280011019.01.T01:intron 40.0%
! GTCATCCACAGTTTTCTTCA+AGG - Chr2:78472094-78472113 None:intergenic 40.0%
! TAAGCTAAGGTTGGATTGGA+AGG - Chr2:78467807-78467826 None:intergenic 40.0%
!! TTACTTTTGCCTTGAGACCT+TGG + Chr2:78468834-78468853 MsG0280011019.01.T01:intron 40.0%
!!! GCTTCTCGTCATTTTTACAG+AGG - Chr2:78469676-78469695 None:intergenic 40.0%
AACATGACCTAAGCGTTCGA+CGG - Chr2:78469385-78469404 None:intergenic 45.0%
AAGTCACTGCACCTTAAAGC+AGG - Chr2:78469517-78469536 None:intergenic 45.0%
AATAAGGACTGGACGGTAAG+AGG - Chr2:78470615-78470634 None:intergenic 45.0%
ACATCAACGGCACAAAGACT+TGG - Chr2:78469635-78469654 None:intergenic 45.0%
ACTTAGTGACCAAGGTCTCA+AGG - Chr2:78468846-78468865 None:intergenic 45.0%
ATCAGCAGTTTATTAGGGCC+TGG + Chr2:78471787-78471806 MsG0280011019.01.T01:intron 45.0%
GACCATGCTAACGAAATGTG+AGG - Chr2:78472051-78472070 None:intergenic 45.0%
GCAGAAGACTCGACAAACAA+GGG - Chr2:78471084-78471103 None:intergenic 45.0%
GTAAGAATAAAAGGGTGGGG+AGG + Chr2:78470305-78470324 MsG0280011019.01.T01:intron 45.0%
GTCCTCACATTTCGTTAGCA+TGG + Chr2:78472046-78472065 MsG0280011019.01.T01:CDS 45.0%
TAACTCAGGCTAGGGAATCA+TGG - Chr2:78470944-78470963 None:intergenic 45.0%
TGCAGAAGACTCGACAAACA+AGG - Chr2:78471085-78471104 None:intergenic 45.0%
! GATCGACTTTGACAAATGGG+AGG + Chr2:78469916-78469935 MsG0280011019.01.T01:CDS 45.0%
! GTTCACGAATGTTTGTTGCC+CGG - Chr2:78467857-78467876 None:intergenic 45.0%
! TTCACGAATGTTTGTTGCCC+GGG - Chr2:78467856-78467875 None:intergenic 45.0%
! TTTTATCCCGTCGAACGCTT+AGG + Chr2:78469375-78469394 MsG0280011019.01.T01:intron 45.0%
!! GCTTTAAGGTGCAGTGACTT+TGG + Chr2:78469517-78469536 MsG0280011019.01.T01:intron 45.0%
!! GGGCTTTGAAGAACACTGAA+TGG + Chr2:78471747-78471766 MsG0280011019.01.T01:intron 45.0%
!!! TTTATTTTTTTAAAACTTAA+CGG + Chr2:78470478-78470497 MsG0280011019.01.T01:intron 5.0%
AACAACCTCGACTGAGAGGA+AGG - Chr2:78469969-78469988 None:intergenic 50.0%
ACAAGGGTTAGACATGACGG+AGG - Chr2:78471068-78471087 None:intergenic 50.0%
ACATGACCTAAGCGTTCGAC+GGG - Chr2:78469384-78469403 None:intergenic 50.0%
AGGAGCTTCCCTCATGTTGA+TGG + Chr2:78468340-78468359 MsG0280011019.01.T01:CDS 50.0%
AGGGTGGGGAGGAATTGAAA+AGG + Chr2:78470316-78470335 MsG0280011019.01.T01:intron 50.0%
CCATTGAGCCCAGGAAAACT+CGG + Chr2:78469210-78469229 MsG0280011019.01.T01:intron 50.0%
CTACCATACGCTCTCCTACT+AGG - Chr2:78470219-78470238 None:intergenic 50.0%
CTCTCAGTCGAGGTTGTTCA+CGG + Chr2:78469971-78469990 MsG0280011019.01.T01:intron 50.0%
GCTAACGAAATGTGAGGACG+TGG - Chr2:78472045-78472064 None:intergenic 50.0%
GGAACCGAGACTGTTAGAGA+TGG + Chr2:78469056-78469075 MsG0280011019.01.T01:intron 50.0%
TCTCAGTCGAGGTTGTTCAC+GGG + Chr2:78469972-78469991 MsG0280011019.01.T01:intron 50.0%
TGCTTAATGTGTGTCGCGAG+TGG + Chr2:78470109-78470128 MsG0280011019.01.T01:intron 50.0%
TGGACGCATGCTTTGTGAAG+TGG - Chr2:78467879-78467898 None:intergenic 50.0%
! CTTCCTAGTAGGAGAGCGTA+TGG + Chr2:78470213-78470232 MsG0280011019.01.T01:intron 50.0%
! TCACGAATGTTTGTTGCCCG+GGG - Chr2:78467855-78467874 None:intergenic 50.0%
CGGATGGTCTGATCACCTAC+AGG + Chr2:78469234-78469253 MsG0280011019.01.T01:intron 55.0%
CGTGAACAACCTCGACTGAG+AGG - Chr2:78469973-78469992 None:intergenic 55.0%
CTCAGTCGAGGTTGTTCACG+GGG + Chr2:78469973-78469992 MsG0280011019.01.T01:intron 55.0%
TCAGTCGAGGTTGTTCACGG+GGG + Chr2:78469974-78469993 MsG0280011019.01.T01:intron 55.0%
TGAGCCCAGGAAAACTCGGT+CGG + Chr2:78469214-78469233 MsG0280011019.01.T01:intron 55.0%
TTGTTCACGGGGGCTTGTAG+AGG + Chr2:78469984-78470003 MsG0280011019.01.T01:intron 55.0%
! ACCATCCGACCGAGTTTTCC+TGG - Chr2:78469222-78469241 None:intergenic 55.0%
! CCATCCGACCGAGTTTTCCT+GGG - Chr2:78469221-78469240 None:intergenic 55.0%
! CCGAGTTTTCCTGGGCTCAA+TGG - Chr2:78469213-78469232 None:intergenic 55.0%
! GGAGGATGAAGGAGCGCAAT+AGG - Chr2:78467918-78467937 None:intergenic 55.0%
! GGATGAAGGAGCGCAATAGG+AGG - Chr2:78467915-78467934 None:intergenic 55.0%
! TGAAGGAGCGCAATAGGAGG+TGG - Chr2:78467912-78467931 None:intergenic 55.0%
ACGCACCTTCCTCTCAGTCG+AGG + Chr2:78469961-78469980 MsG0280011019.01.T01:CDS 60.0%
CCCAGGAAAACTCGGTCGGA+TGG + Chr2:78469218-78469237 MsG0280011019.01.T01:intron 60.0%
CCTCAAGCTCCATTGAGCCC+AGG + Chr2:78469201-78469220 MsG0280011019.01.T01:intron 60.0%
CCTGGGCTCAATGGAGCTTG+AGG - Chr2:78469204-78469223 None:intergenic 60.0%
GGGGGCTGAGACTTCCTAGT+AGG + Chr2:78470202-78470221 MsG0280011019.01.T01:intron 60.0%
TTTGTTGCCCGGGGTGGCAA+GGG - Chr2:78467846-78467865 None:intergenic 60.0%
! AGGAGCGCAATAGGAGGTGG+CGG - Chr2:78467909-78467928 None:intergenic 60.0%
! CGAATGTTTGTTGCCCGGGG+TGG - Chr2:78467852-78467871 None:intergenic 60.0%
! GCAATAGGAGGTGGCGGCAA+GGG - Chr2:78467903-78467922 None:intergenic 60.0%
! CGCAATAGGAGGTGGCGGCA+AGG - Chr2:78467904-78467923 None:intergenic 65.0%
! GTTTGTTGCCCGGGGTGGCA+AGG - Chr2:78467847-78467866 None:intergenic 65.0%
ACGTGAGCCCTTGCCACCCC+GGG + Chr2:78467836-78467855 MsG0280011019.01.T01:CDS 70.0%
GACGTGAGCCCTTGCCACCC+CGG + Chr2:78467835-78467854 MsG0280011019.01.T01:CDS 70.0%
! TAGGAGGTGGCGGCAAGGGC+TGG - Chr2:78467899-78467918 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr2 gene 78467744 78472377 78467744 ID=MsG0280011019.01;Name=MsG0280011019.01
Chr2 mRNA 78467744 78472377 78467744 ID=MsG0280011019.01.T01;Parent=MsG0280011019.01;Name=MsG0280011019.01.T01;_AED=0.50;_eAED=0.53;_QI=0|0.6|0.5|1|1|1|6|141|333
Chr2 exon 78467744 78467989 78467744 ID=MsG0280011019.01.T01:exon:21251;Parent=MsG0280011019.01.T01
Chr2 exon 78468265 78468391 78468265 ID=MsG0280011019.01.T01:exon:21252;Parent=MsG0280011019.01.T01
Chr2 exon 78469525 78469816 78469525 ID=MsG0280011019.01.T01:exon:21253;Parent=MsG0280011019.01.T01
Chr2 exon 78469910 78469982 78469910 ID=MsG0280011019.01.T01:exon:21254;Parent=MsG0280011019.01.T01
Chr2 exon 78471657 78471722 78471657 ID=MsG0280011019.01.T01:exon:21255;Parent=MsG0280011019.01.T01
Chr2 exon 78472039 78472377 78472039 ID=MsG0280011019.01.T01:exon:21256;Parent=MsG0280011019.01.T01
Chr2 CDS 78467744 78467989 78467744 ID=MsG0280011019.01.T01:cds;Parent=MsG0280011019.01.T01
Chr2 CDS 78468265 78468391 78468265 ID=MsG0280011019.01.T01:cds;Parent=MsG0280011019.01.T01
Chr2 CDS 78469525 78469816 78469525 ID=MsG0280011019.01.T01:cds;Parent=MsG0280011019.01.T01
Chr2 CDS 78469910 78469982 78469910 ID=MsG0280011019.01.T01:cds;Parent=MsG0280011019.01.T01
Chr2 CDS 78471657 78471722 78471657 ID=MsG0280011019.01.T01:cds;Parent=MsG0280011019.01.T01
Chr2 CDS 78472039 78472236 78472039 ID=MsG0280011019.01.T01:cds;Parent=MsG0280011019.01.T01
Chr2 three_prime_UTR 78472237 78472377 78472237 ID=MsG0280011019.01.T01:three_prime_utr;Parent=MsG0280011019.01.T01
Gene Sequence

>MsG0280011019.01.T01

ATGGAGGCATTAATGCAATGTTATGGGGATGGATCTTCTGATTCAGATTCTGAATCTTCTCCTTCCAATCCAACCTTAGCTTATGCAGAAAGACGTGAGCCCTTGCCACCCCGGGCAACAAACATTCGTGAACCACTTCACAAAGCATGCGTCCAGCCCTTGCCGCCACCTCCTATTGCGCTCCTTCATCCTCCAAATTTTCTTGGTATGCTGGGTTTTAAGCTGATTTTCTTACTCTTTACAAGGTGCCTTTTGATGATGAATGTTCATTGTTGTGATGAAGGGCCTCAAGATATTCAGATAAGCCAGACAACTAAAGTTAGGAGCTTCCCTCATGTTGATGGTAACTATGCCTTACATGTATACATACCAAGTGCAGTGACTTTGGACTCAGACTGCAATTGTTCTGTTTCAGTTAATATTTCATCTTCATCGAAAAAAGAAGTAGCCGCTTTCTTGAAGAAAATATCATCTCTGCATCCAAGTCTTTGTGCCGTTGATGTTGATGTCCCACTTAACGTCCTCTGTAAAAATGACGAGAAGCTTGAACAACTTTTACTAGGAAGGGAGTTTCACATAAGTTTGGGAAGAACTGTTCCAATACGAGTACATCAGATTGACTCAGTGGTCACAATGCTAAAGCAGAAGCTTCAGACGCAACCCCTATACTGGATCGACTTTGACAAATGGGAGGTTTTTGTTAATGATGATCGCACACGCACCTTCCTCTCAGTCGAGATAAGGAAGCAAATTGAAGCAGTCAATGCAATTTACAAACTTCATAACCTTCCTGAATTTTACGAGGATCCACGTCCTCACATTTCGTTAGCATGGTCATTGGGCGACACTGCCGATTCCTTGAAGAAAACTGTGGATGACGAAATCAAGAAATGTGTTTCTGGGAAATCATCGAAGAAGTGTATTTTTACCTGTAAATTCAAAGGTATCGAATGTAAGATTGGTCAGAAAACTTACAAAATATGTAAGATTTCAGATAGATGA
Protein sequence

>MsG0280011019.01.T01

MEALMQCYGDGSSDSDSESSPSNPTLAYAERREPLPPRATNIREPLHKACVQPLPPPPIALLHPPNFLGMLGFKLIFLLFTRCLLMMNVHCCDEGPQDIQISQTTKVRSFPHVDGNYALHVYIPSAVTLDSDCNCSVSVNISSSSKKEVAAFLKKISSLHPSLCAVDVDVPLNVLCKNDEKLEQLLLGREFHISLGRTVPIRVHQIDSVVTMLKQKLQTQPLYWIDFDKWEVFVNDDRTRTFLSVEIRKQIEAVNAIYKLHNLPEFYEDPRPHISLAWSLGDTADSLKKTVDDEIKKCVSGKSSKKCIFTCKFKGIECKIGQKTYKICKISDR*