AlfalfaGEDB | MsG0880045705.01

M. sativa cultivar ZhongmuNo.1 / MsG0880045705.01

KEGG: K19600
GO: -
PFAM: PF01167

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880045705.01.T01	MCI09404.1	98.611	72	1	0	1	72	44	115	1.79E-44	149

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880045705.01.T01	Q6Z2G9	90.141	71	7	0	2	72	358	428	5.75E-39	135

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880045705.01.T01	A0A072TSP9	98.611	72	1	0	1	72	341	412	6.53e-42	150
MsG0880045705.01.T01	A0A392PC89	98.611	72	1	0	1	72	44	115	8.56e-45	149

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000144.01	MsG0880045705.01	-0.818809	1.615029e-52	1.221312e-49
MsG0180000155.01	MsG0880045705.01	-0.802129	6.495101e-49	3.156642e-46
MsG0180000400.01	MsG0880045705.01	0.824481	7.886435e-54	6.993923e-51
MsG0180001886.01	MsG0880045705.01	-0.802470	5.525360e-49	2.708809e-46
MsG0180002686.01	MsG0880045705.01	0.829303	5.550318e-55	5.659317e-52
MsG0880045145.01	MsG0880045705.01	-0.809203	2.124857e-50	1.239832e-47
MsG0880045480.01	MsG0880045705.01	0.830972	2.172881e-55	2.325600e-52
MsG0880045656.01	MsG0880045705.01	-0.818651	1.753913e-52	1.320555e-49
MsG0880045705.01	MsG0880047428.01	-0.806091	9.733914e-50	5.236775e-47
MsG0880045705.01	MsG0880047470.01	-0.800256	1.569900e-48	7.276174e-46
MsG0880045705.01	MsG0880047611.01	0.804539	2.058333e-49	1.063999e-46
MsG0180003303.01	MsG0880045705.01	-0.806624	7.514239e-50	4.097727e-47
MsG0180003793.01	MsG0880045705.01	-0.804738	1.870636e-49	9.720665e-47
MsG0180004016.01	MsG0880045705.01	-0.829569	4.783619e-55	4.914437e-52
MsG0180004293.01	MsG0880045705.01	0.814141	1.792195e-51	1.192894e-48
MsG0180004854.01	MsG0880045705.01	-0.809671	1.686343e-50	9.962898e-48
MsG0180005049.01	MsG0880045705.01	-0.823869	1.097914e-53	9.569820e-51
MsG0180005543.01	MsG0880045705.01	0.814983	1.166592e-51	7.943700e-49
MsG0180006050.01	MsG0880045705.01	-0.806526	7.881657e-50	4.287508e-47
MsG0780041223.01	MsG0880045705.01	-0.805143	1.538904e-49	8.080271e-47
MsG0780041268.01	MsG0880045705.01	-0.835728	1.417338e-56	1.748347e-53
MsG0880042399.01	MsG0880045705.01	0.811093	8.318652e-51	5.102652e-48
MsG0880043162.01	MsG0880045705.01	-0.804823	1.795157e-49	9.348917e-47
MsG0280011019.01	MsG0880045705.01	0.812225	4.718621e-51	2.983176e-48
MsG0380013579.01	MsG0880045705.01	-0.812205	4.766888e-51	3.011907e-48
MsG0680031597.01	MsG0880045705.01	0.812806	3.522880e-51	2.261495e-48
MsG0680031628.01	MsG0880045705.01	-0.834152	3.535151e-56	4.158963e-53
MsG0680034913.01	MsG0880045705.01	-0.809056	2.284966e-50	1.327933e-47
MsG0480018477.01	MsG0880045705.01	-0.813216	2.863386e-51	1.858519e-48
MsG0480019984.01	MsG0880045705.01	0.845159	4.833334e-59	7.982279e-56
MsG0480020675.01	MsG0880045705.01	-0.822195	2.697118e-53	2.241800e-50
MsG0480021876.01	MsG0880045705.01	0.814017	1.908185e-51	1.265851e-48
MsG0480023136.01	MsG0880045705.01	0.844229	8.603115e-59	1.379867e-55
MsG0480023841.01	MsG0880045705.01	0.840098	1.066768e-57	1.503757e-54
MsG0580024339.01	MsG0880045705.01	-0.809016	2.330391e-50	1.352852e-47
MsG0580025363.01	MsG0880045705.01	0.815613	8.451504e-52	5.855460e-49
MsG0580025756.01	MsG0880045705.01	-0.804540	2.057088e-49	1.063396e-46
MsG0380015598.01	MsG0880045705.01	0.829418	5.205801e-55	5.324942e-52
MsG0380016005.01	MsG0880045705.01	-0.810751	9.862940e-51	5.995302e-48
MsG0380016342.01	MsG0880045705.01	0.841190	5.521983e-58	8.050757e-55
MsG0380017797.01	MsG0880045705.01	-0.830859	2.316633e-55	2.471157e-52
MsG0680030350.01	MsG0880045705.01	-0.805495	1.298953e-49	6.882049e-47
MsG0280006519.01	MsG0880045705.01	-0.801798	7.596186e-49	3.660865e-46
MsG0280006993.01	MsG0880045705.01	-0.802355	5.835793e-49	2.852762e-46
MsG0280009637.01	MsG0880045705.01	0.802172	6.363928e-49	3.096299e-46
MsG0780036078.01	MsG0880045705.01	-0.823230	1.548936e-53	1.325856e-50
MsG0780036247.01	MsG0880045705.01	0.858720	6.785000e-63	1.748255e-59
MsG0780036544.01	MsG0880045705.01	0.836393	9.608953e-57	1.209302e-53
MsG0780037240.01	MsG0880045705.01	-0.805678	1.189019e-49	6.329556e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880045705.01.T01	MTR_8g077195	98.611	72	1	0	1	72	341	412	1.66e-45	150
MsG0880045705.01.T01	MTR_5g020580	90.141	71	7	0	2	72	346	416	4.17e-41	138
MsG0880045705.01.T01	MTR_1g022370	88.732	71	8	0	2	72	352	422	5.84e-40	135
MsG0880045705.01.T01	MTR_8g077650	90.476	63	6	0	2	64	266	328	1.48e-34	119
MsG0880045705.01.T01	MTR_1g088960	77.465	71	14	1	2	72	334	402	1.33e-31	113
MsG0880045705.01.T01	MTR_2g020280	69.014	71	20	1	2	72	226	294	5.92e-30	107
MsG0880045705.01.T01	MTR_2g007930	67.532	77	19	1	2	72	351	427	1.24e-29	108
MsG0880045705.01.T01	MTR_2g076200	73.239	71	19	0	2	72	301	371	1.68e-29	107
MsG0880045705.01.T01	MTR_2g020280	69.014	71	20	1	2	72	325	393	1.93e-29	107
MsG0880045705.01.T01	MTR_1g098790	63.095	84	17	2	2	72	349	431	6.05e-27	100
MsG0880045705.01.T01	MTR_8g077620	73.529	68	13	2	1	63	317	384	3.29e-26	98.6
MsG0880045705.01.T01	MTR_8g077675	72.727	66	16	1	2	65	330	395	3.71e-24	93.2
MsG0880045705.01.T01	MTR_2g076210	65.217	69	24	0	4	72	299	367	3.50e-23	90.1
MsG0880045705.01.T01	MTR_8g077640	66.667	63	20	1	1	62	321	383	6.57e-20	81.6
MsG0880045705.01.T01	MTR_2g105390	46.875	64	25	1	8	71	305	359	4.48e-13	62.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880045705.01.T01	AT2G18280	81.690	71	13	0	2	72	323	393	2.19e-35	123
MsG0880045705.01.T01	AT2G18280	81.690	71	13	0	2	72	323	393	2.19e-35	123
MsG0880045705.01.T01	AT1G47270	75.676	74	15	1	2	72	339	412	1.52e-33	118
MsG0880045705.01.T01	AT1G47270	75.676	74	15	1	2	72	315	388	1.58e-33	118
MsG0880045705.01.T01	AT1G47270	75.676	74	15	1	2	72	340	413	1.73e-33	118
MsG0880045705.01.T01	AT1G25280	67.073	82	16	1	2	72	186	267	3.96e-30	107
MsG0880045705.01.T01	AT1G25280	67.073	82	16	1	2	72	186	267	3.96e-30	107
MsG0880045705.01.T01	AT2G47900	73.239	71	17	1	2	72	339	407	4.08e-30	109
MsG0880045705.01.T01	AT2G47900	73.239	71	17	1	2	72	338	406	4.12e-30	109
MsG0880045705.01.T01	AT2G47900	73.239	71	17	1	2	72	338	406	4.12e-30	109
MsG0880045705.01.T01	AT1G53320	73.239	71	19	0	2	72	309	379	1.41e-29	107
MsG0880045705.01.T01	AT1G25280	67.073	82	16	1	2	72	364	445	5.11e-29	107
MsG0880045705.01.T01	AT3G06380	71.831	71	17	1	2	72	221	288	5.59e-29	104
MsG0880045705.01.T01	AT5G18680	69.333	75	19	2	2	72	223	297	1.67e-28	103
MsG0880045705.01.T01	AT3G06380	71.831	71	17	1	2	72	313	380	1.84e-28	104
MsG0880045705.01.T01	AT5G18680	69.333	75	19	2	2	72	315	389	8.26e-28	102
MsG0880045705.01.T01	AT1G76900	56.322	87	22	1	2	72	369	455	4.61e-24	93.2
MsG0880045705.01.T01	AT1G76900	56.322	87	22	1	2	72	369	455	4.61e-24	93.2
MsG0880045705.01.T01	AT1G76900	56.322	87	22	1	2	72	369	455	4.61e-24	93.2
MsG0880045705.01.T01	AT1G43640	55.056	89	22	2	2	72	275	363	4.72e-24	92.4
MsG0880045705.01.T01	AT1G43640	55.056	89	22	2	2	72	341	429	7.04e-24	92.8
MsG0880045705.01.T01	AT1G16070	41.538	65	29	1	7	71	340	395	3.86e-11	57.0

Find 16 sgRNAs with CRISPR-Local

Find 13 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATCTTACAGTTTGGAAAGAT+TGG	0.295811	8:+63049144	MsG0880045705.01.T01:CDS
CTCTCATTCACAAGCAGGTT+TGG	0.347646	8:-63049247	None:intergenic
GAAAGGGTAATCTTACAGTT+TGG	0.371540	8:+63049135	MsG0880045705.01.T01:CDS
TTTCTTCCGCTGAGCAAGAA+AGG	0.454492	8:+63049118	MsG0880045705.01.T01:CDS
TCAAGCAGATTGCAAAGGCT+TGG	0.460094	8:-63049212	None:intergenic
CACAGCTCTCATTCACAAGC+AGG	0.495782	8:-63049252	None:intergenic
GCAGATTGCAAAGGCTTGGA+AGG	0.525320	8:-63049208	None:intergenic
TTCTTCCGCTGAGCAAGAAA+GGG	0.530855	8:+63049119	MsG0880045705.01.T01:CDS
GCTTGTCAAGCAGATTGCAA+AGG	0.591302	8:-63049217	None:intergenic
AAGGCTTGGAAGGCAGAGAG+TGG	0.610317	8:-63049198	None:intergenic
GAGTGGGTAAGAGTAGTCCA+TGG	0.613248	8:-63049181	None:intergenic
CTTCATGGGTCGTGTTACAG+TGG	0.629354	8:+63049044	None:intergenic
GCGGAAGAAACATTATGAGA+CGG	0.646089	8:-63049105	None:intergenic
TGGAAAAGATATATTCACCA+TGG	0.647072	8:+63049164	MsG0880045705.01.T01:CDS
AGGCTTGGAAGGCAGAGAGT+GGG	0.650720	8:-63049197	None:intergenic
GATTACCCTTTCTTGCTCAG+CGG	0.690765	8:-63049124	None:intergenic

CRISPR-GE

sgRNA_sequence	Strand	Position	Region	GC_content
ATCTTACAGTTTGGAAAGAT+TGG	+	Chr8:63049144-63049163	MsG0880045705.01.T01:CDS	30.0%
TGGAAAAGATATATTCACCA+TGG	+	Chr8:63049164-63049183	MsG0880045705.01.T01:CDS	30.0%
GAAAGGGTAATCTTACAGTT+TGG	+	Chr8:63049135-63049154	MsG0880045705.01.T01:CDS	35.0%
GCGGAAGAAACATTATGAGA+CGG	-	Chr8:63049108-63049127	None:intergenic	40.0%
GATTACCCTTTCTTGCTCAG+CGG	-	Chr8:63049127-63049146	None:intergenic	45.0%
GCTTGTCAAGCAGATTGCAA+AGG	-	Chr8:63049220-63049239	None:intergenic	45.0%
TCAAGCAGATTGCAAAGGCT+TGG	-	Chr8:63049215-63049234	None:intergenic	45.0%
TTCTTCCGCTGAGCAAGAAA+GGG	+	Chr8:63049119-63049138	MsG0880045705.01.T01:CDS	45.0%
TTTCTTCCGCTGAGCAAGAA+AGG	+	Chr8:63049118-63049137	MsG0880045705.01.T01:CDS	45.0%
GAGTGGGTAAGAGTAGTCCA+TGG	-	Chr8:63049184-63049203	None:intergenic	50.0%
GCAGATTGCAAAGGCTTGGA+AGG	-	Chr8:63049211-63049230	None:intergenic	50.0%
AAGGCTTGGAAGGCAGAGAG+TGG	-	Chr8:63049201-63049220	None:intergenic	55.0%
AGGCTTGGAAGGCAGAGAGT+GGG	-	Chr8:63049200-63049219	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	63049048	63049266	63049048	ID=MsG0880045705.01;Name=MsG0880045705.01
Chr8	mRNA	63049048	63049266	63049048	ID=MsG0880045705.01.T01;Parent=MsG0880045705.01;Name=MsG0880045705.01.T01;_AED=0.46;_eAED=0.46;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|72
Chr8	exon	63049048	63049266	63049048	ID=MsG0880045705.01.T01:exon:20011;Parent=MsG0880045705.01.T01
Chr8	CDS	63049048	63049266	63049048	ID=MsG0880045705.01.T01:cds;Parent=MsG0880045705.01.T01

Gene Sequence	>MsG0880045705.01.T01 ATGGGTCGTGTTACAGTGGCATCTGTTAAGAACTTTCAACTTGTAGCTTCTGTTGATCCGTCTCATAATGTTTCTTCCGCTGAGCAAGAAAGGGTAATCTTACAGTTTGGAAAGATTGGAAAAGATATATTCACCATGGACTACTCTTACCCACTCTCTGCCTTCCAAGCCTTTGCAATCTGCTTGACAAGCTTTGATACCAAACCTGCTTGTGAATGA
Protein sequence	>MsG0880045705.01.T01 MGRVTVASVKNFQLVASVDPSHNVSSAEQERVILQFGKIGKDIFTMDYSYPLSAFQAFAICLTSFDTKPACE*