Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280011051.01.T01 | XP_024631646.1 | 96.923 | 65 | 2 | 0 | 1 | 65 | 68 | 132 | 1.52E-33 | 127 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280011051.01.T01 | A0A396JHF9 | 96.923 | 65 | 2 | 0 | 1 | 65 | 68 | 132 | 7.26e-34 | 127 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180001486.01 | MsG0280011051.01 | 0.803139 | 4.019304e-49 | 2.004339e-46 |
| MsG0180005127.01 | MsG0280011051.01 | 0.845503 | 3.898767e-59 | 6.510722e-56 |
| MsG0180005146.01 | MsG0280011051.01 | 0.840921 | 6.497546e-58 | 9.395102e-55 |
| MsG0280011050.01 | MsG0280011051.01 | 0.825115 | 5.587898e-54 | 5.046853e-51 |
| MsG0280011051.01 | MsG0480021226.01 | 0.815932 | 7.173538e-52 | 5.013151e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 12 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGACCCCTGCTAATTTATT+TGG | 0.145139 | 2:+78886071 | None:intergenic |
| GTTACTGTGGCTTTGCATCT+AGG | 0.282488 | 2:-78886185 | MsG0280011051.01.T01:CDS |
| CTTCATATTCATCATTTCCA+TGG | 0.449667 | 2:+78886253 | None:intergenic |
| TTACTGTGGCTTTGCATCTA+GGG | 0.458041 | 2:-78886184 | MsG0280011051.01.T01:CDS |
| ACCCCTGCTAATTTATTTGG+TGG | 0.463538 | 2:+78886074 | None:intergenic |
| TGTCCACCAAATAAATTAGC+AGG | 0.481720 | 2:-78886077 | MsG0280011051.01.T01:CDS |
| AAATCAGATGAACTTATACT+TGG | 0.487140 | 2:+78886158 | None:intergenic |
| AAATTAGCAGGGGTCAGTAT+TGG | 0.506191 | 2:-78886065 | MsG0280011051.01.T01:CDS |
| GGACCGATTAGAATCTGAGC+AGG | 0.523985 | 2:+78886033 | None:intergenic |
| CATGGAAATGATGAATATGA+AGG | 0.524476 | 2:-78886252 | MsG0280011051.01.T01:CDS |
| ATTAGAATCTGAGCAGGGGT+AGG | 0.550899 | 2:+78886039 | None:intergenic |
| TTAGAATCTGAGCAGGGGTA+GGG | 0.569587 | 2:+78886040 | None:intergenic |
| GTCCACCAAATAAATTAGCA+GGG | 0.606848 | 2:-78886076 | MsG0280011051.01.T01:CDS |
| GACCGATTAGAATCTGAGCA+GGG | 0.623713 | 2:+78886034 | None:intergenic |
| AGATGATGATGCCGTTACTG+TGG | 0.634808 | 2:-78886198 | MsG0280011051.01.T01:CDS |
| TAGATGCAAAGCCACAGTAA+CGG | 0.646015 | 2:+78886187 | None:intergenic |
| TCCACCAAATAAATTAGCAG+GGG | 0.677713 | 2:-78886075 | MsG0280011051.01.T01:CDS |
| ACCGATTAGAATCTGAGCAG+GGG | 0.690964 | 2:+78886035 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAATCAGATGAACTTATACT+TGG | + | Chr2:78886136-78886155 | None:intergenic | 25.0% |
| GTCCACCAAATAAATTAGCA+GGG | - | Chr2:78886215-78886234 | MsG0280011051.01.T01:CDS | 35.0% | |
| TCCACCAAATAAATTAGCAG+GGG | - | Chr2:78886216-78886235 | MsG0280011051.01.T01:CDS | 35.0% | |
| TGTCCACCAAATAAATTAGC+AGG | - | Chr2:78886214-78886233 | MsG0280011051.01.T01:CDS | 35.0% | |
| AAATTAGCAGGGGTCAGTAT+TGG | - | Chr2:78886226-78886245 | MsG0280011051.01.T01:CDS | 40.0% | |
| ACCCCTGCTAATTTATTTGG+TGG | + | Chr2:78886220-78886239 | None:intergenic | 40.0% | |
| CTGACCCCTGCTAATTTATT+TGG | + | Chr2:78886223-78886242 | None:intergenic | 40.0% | |
| TAGATGCAAAGCCACAGTAA+CGG | + | Chr2:78886107-78886126 | None:intergenic | 40.0% | |
| ! | TTACTGTGGCTTTGCATCTA+GGG | - | Chr2:78886107-78886126 | MsG0280011051.01.T01:CDS | 40.0% |
| TTAGAATCTGAGCAGGGGTA+GGG | + | Chr2:78886254-78886273 | None:intergenic | 45.0% | |
| ! | AGATGATGATGCCGTTACTG+TGG | - | Chr2:78886093-78886112 | MsG0280011051.01.T01:CDS | 45.0% |
| ! | GTTACTGTGGCTTTGCATCT+AGG | - | Chr2:78886106-78886125 | MsG0280011051.01.T01:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 78886040 | 78886273 | 78886040 | ID=MsG0280011051.01;Name=MsG0280011051.01 |
| Chr2 | mRNA | 78886040 | 78886273 | 78886040 | ID=MsG0280011051.01.T01;Parent=MsG0280011051.01;Name=MsG0280011051.01.T01;_AED=0.46;_eAED=0.46;_QI=0|-1|0|1|-1|1|1|0|77 |
| Chr2 | exon | 78886040 | 78886273 | 78886040 | ID=MsG0280011051.01.T01:exon:17386;Parent=MsG0280011051.01.T01 |
| Chr2 | CDS | 78886040 | 78886273 | 78886040 | ID=MsG0280011051.01.T01:cds;Parent=MsG0280011051.01.T01 |
| Gene Sequence |
| Protein sequence |
