AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280011050.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280011050.01.T01 MTR_1g093095 76.378 127 27 1 1 124 134 260 2.92e-70 213
MsG0280011050.01.T01 MTR_4g059870 36.364 110 63 4 8 113 85 191 8.75e-14 67.8
MsG0280011050.01.T01 MTR_1g112270 40.000 85 46 3 30 113 127 207 1.60e-13 67.0
MsG0280011050.01.T01 MTR_1g112270 40.000 85 46 3 30 113 116 196 2.07e-13 66.6
MsG0280011050.01.T01 MTR_8g017210 38.043 92 51 4 24 113 127 214 6.83e-12 62.4
MsG0280011050.01.T01 MTR_1g094115 38.462 91 50 4 25 113 96 182 7.76e-12 62.0
MsG0280011050.01.T01 MTR_1g094115 38.462 91 50 4 25 113 113 199 8.36e-12 62.0
MsG0280011050.01.T01 MTR_2g093960 35.922 103 62 3 12 113 86 185 1.14e-11 61.6
MsG0280011050.01.T01 MTR_1g016010 35.577 104 61 4 12 113 95 194 2.19e-11 60.8
MsG0280011050.01.T01 MTR_2g099990 38.043 92 51 4 24 113 111 198 4.17e-11 60.1
MsG0280011050.01.T01 MTR_2g099990 38.043 92 51 4 24 113 42 129 4.51e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280011050.01.T01 AT3G57670 86.093 151 14 3 1 148 236 382 5.33e-89 264
MsG0280011050.01.T01 AT1G51220 82.270 141 25 0 1 141 197 337 5.76e-83 247
MsG0280011050.01.T01 AT3G20880 80.142 141 23 1 1 141 275 410 6.84e-82 247
MsG0280011050.01.T01 AT1G13290 80.952 126 22 2 1 124 120 245 1.05e-72 220
MsG0280011050.01.T01 AT1G08290 80.165 121 23 1 1 120 199 319 6.05e-70 214
MsG0280011050.01.T01 AT1G34790 80.833 120 23 0 1 120 164 283 6.19e-70 213
MsG0280011050.01.T01 AT1G34370 35.714 154 87 5 1 144 53 204 4.94e-20 84.0
MsG0280011050.01.T01 AT1G34370 38.060 134 72 4 1 125 263 394 8.47e-20 85.1
MsG0280011050.01.T01 AT1G34370 38.060 134 72 4 1 125 263 394 8.47e-20 85.1
MsG0280011050.01.T01 AT5G22890 38.931 131 67 6 1 121 236 363 1.98e-16 75.1
MsG0280011050.01.T01 AT2G02080 35.455 110 63 4 8 113 101 206 4.66e-14 68.6
MsG0280011050.01.T01 AT2G02080 35.455 110 63 4 8 113 101 206 4.66e-14 68.6
MsG0280011050.01.T01 AT2G02080 35.455 110 63 4 8 113 101 206 4.66e-14 68.6
MsG0280011050.01.T01 AT2G02080 35.455 110 63 4 8 113 101 206 4.66e-14 68.6
MsG0280011050.01.T01 AT5G66730 36.937 111 60 6 8 113 79 184 1.59e-13 67.0
MsG0280011050.01.T01 AT1G55110 38.710 93 51 4 23 113 128 216 3.90e-12 63.2
MsG0280011050.01.T01 AT1G55110 38.710 93 51 4 23 113 128 216 3.90e-12 63.2
MsG0280011050.01.T01 AT1G55110 38.710 93 51 4 23 113 128 216 3.90e-12 63.2
MsG0280011050.01.T01 AT5G03150 38.043 92 53 3 23 113 118 206 5.54e-12 62.8
MsG0280011050.01.T01 AT1G14580 40.217 92 49 4 24 113 118 205 8.17e-12 62.0
MsG0280011050.01.T01 AT1G14580 40.217 92 49 4 24 113 118 205 8.17e-12 62.0
MsG0280011050.01.T01 AT1G14580 40.217 92 49 4 24 113 132 219 9.97e-12 62.0
MsG0280011050.01.T01 AT2G02070 40.000 85 46 3 30 113 124 204 1.25e-11 61.6
MsG0280011050.01.T01 AT2G02070 40.000 85 46 3 30 113 124 204 1.25e-11 61.6
MsG0280011050.01.T01 AT2G02080 40.000 85 46 3 30 113 49 129 1.34e-11 61.6
MsG0280011050.01.T01 AT2G02080 40.000 85 46 3 30 113 49 129 1.34e-11 61.6
MsG0280011050.01.T01 AT3G50700 39.474 76 44 2 39 113 112 186 1.50e-11 61.2
MsG0280011050.01.T01 AT3G45260 42.105 76 42 2 39 113 118 192 9.51e-11 58.9
MsG0280011050.01.T01 AT3G45260 42.105 76 42 2 39 113 118 192 9.51e-11 58.9

Find 23 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTGTTCCTCTTAGAGATTC+TGG 0.183675 2:+78884465 None:intergenic
CAACACTTTCTACACATAAA+TGG 0.204972 2:+78884306 None:intergenic
AAAGATCATATCAAGGCTTT+TGG 0.250816 2:-78884180 MsG0280011050.01.T01:CDS
GGAAATGGACATAAAGCTTA+TGG 0.262958 2:-78884159 MsG0280011050.01.T01:CDS
AACTTTGGTATTGTTGTTGT+GGG 0.402964 2:-78884230 MsG0280011050.01.T01:CDS
TGCAAAGCATTTGCAGTAAG+AGG 0.436467 2:-78884285 MsG0280011050.01.T01:CDS
AAACTTTGGTATTGTTGTTG+TGG 0.458245 2:-78884231 MsG0280011050.01.T01:CDS
CCATGTTATTGTTGTGCACC+TGG 0.466023 2:-78884420 MsG0280011050.01.T01:CDS
ATCACTTAAAGATCATATCA+AGG 0.535427 2:-78884187 MsG0280011050.01.T01:CDS
ACATGGATCACAATATAGAA+AGG 0.537364 2:-78884490 MsG0280011050.01.T01:CDS
CCAGGTGCACAACAATAACA+TGG 0.547993 2:+78884420 None:intergenic
TCAATGTTGTTCTTGCATCC+AGG 0.558572 2:+78884402 None:intergenic
CATGGATCACAATATAGAAA+GGG 0.565142 2:-78884489 MsG0280011050.01.T01:CDS
ACACATTACAAGAGAAAGCA+TGG 0.565234 2:-78884336 MsG0280011050.01.T01:CDS
GGGAGTCTTAGCATTGCTGT+TGG 0.566939 2:+78884441 None:intergenic
TAGATGCATATGTGGGGACA+TGG 0.582440 2:-78884507 None:intergenic
AAGGGACCAGAATCTCTAAG+AGG 0.583893 2:-78884471 MsG0280011050.01.T01:CDS
AGTGTTCTAAAATCTTTGAG+TGG 0.612744 2:+78884363 None:intergenic
CATTTGCAGTAAGAGGTGAC+TGG 0.624837 2:-78884278 MsG0280011050.01.T01:CDS
CAAGATCAAGATGATGAACA+TGG 0.669051 2:-78884117 MsG0280011050.01.T01:CDS
AATATATAGATGCATATGTG+GGG 0.677061 2:-78884513 None:intergenic
CAGGTGCACAACAATAACAT+GGG 0.695885 2:+78884421 None:intergenic
GATCAAGATGATGAACATGG+TGG 0.762207 2:-78884114 MsG0280011050.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ATCACTTAAAGATCATATCA+AGG - Chr2:78884385-78884404 MsG0280011050.01.T01:CDS 25.0%
!! TGATCTTTTGTGCTTAAAAT+CGG + Chr2:78884369-78884388 None:intergenic 25.0%
ACATGGATCACAATATAGAA+AGG - Chr2:78884082-78884101 MsG0280011050.01.T01:CDS 30.0%
CAACACTTTCTACACATAAA+TGG + Chr2:78884269-78884288 None:intergenic 30.0%
CATGGATCACAATATAGAAA+GGG - Chr2:78884083-78884102 MsG0280011050.01.T01:CDS 30.0%
! AAACTTTGGTATTGTTGTTG+TGG - Chr2:78884341-78884360 MsG0280011050.01.T01:CDS 30.0%
! AAAGATCATATCAAGGCTTT+TGG - Chr2:78884392-78884411 MsG0280011050.01.T01:CDS 30.0%
! AACTTTGGTATTGTTGTTGT+GGG - Chr2:78884342-78884361 MsG0280011050.01.T01:CDS 30.0%
!! AGTGTTCTAAAATCTTTGAG+TGG + Chr2:78884212-78884231 None:intergenic 30.0%
ACACATTACAAGAGAAAGCA+TGG - Chr2:78884236-78884255 MsG0280011050.01.T01:CDS 35.0%
AGAAAAACTGTGGGAAACTT+TGG - Chr2:78884327-78884346 MsG0280011050.01.T01:CDS 35.0%
CAAGATCAAGATGATGAACA+TGG - Chr2:78884455-78884474 MsG0280011050.01.T01:CDS 35.0%
GGAAATGGACATAAAGCTTA+TGG - Chr2:78884413-78884432 MsG0280011050.01.T01:CDS 35.0%
!! CATATCAAGGCTTTTGGAAA+TGG - Chr2:78884398-78884417 MsG0280011050.01.T01:CDS 35.0%
AGAACTCACGAGAAAAACTG+TGG - Chr2:78884317-78884336 MsG0280011050.01.T01:CDS 40.0%
CAGGTGCACAACAATAACAT+GGG + Chr2:78884154-78884173 None:intergenic 40.0%
GAACTCACGAGAAAAACTGT+GGG - Chr2:78884318-78884337 MsG0280011050.01.T01:CDS 40.0%
GATCAAGATGATGAACATGG+TGG - Chr2:78884458-78884477 MsG0280011050.01.T01:CDS 40.0%
TCAATGTTGTTCTTGCATCC+AGG + Chr2:78884173-78884192 None:intergenic 40.0%
TGTGTTCCTCTTAGAGATTC+TGG + Chr2:78884110-78884129 None:intergenic 40.0%
! TGCAAAGCATTTGCAGTAAG+AGG - Chr2:78884287-78884306 MsG0280011050.01.T01:CDS 40.0%
!! TCTTTGAGTGGTTTTGCTCT+TGG + Chr2:78884200-78884219 None:intergenic 40.0%
AAGGGACCAGAATCTCTAAG+AGG - Chr2:78884101-78884120 MsG0280011050.01.T01:CDS 45.0%
CATTTGCAGTAAGAGGTGAC+TGG - Chr2:78884294-78884313 MsG0280011050.01.T01:CDS 45.0%
CCAGGTGCACAACAATAACA+TGG + Chr2:78884155-78884174 None:intergenic 45.0%
CCATGTTATTGTTGTGCACC+TGG - Chr2:78884152-78884171 MsG0280011050.01.T01:CDS 45.0%
GGGAGTCTTAGCATTGCTGT+TGG + Chr2:78884134-78884153 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
Chr2 gene 78884074 78884520 78884074 ID=MsG0280011050.01;Name=MsG0280011050.01
Chr2 mRNA 78884074 78884520 78884074 ID=MsG0280011050.01.T01;Parent=MsG0280011050.01;Name=MsG0280011050.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|148
Chr2 exon 78884074 78884520 78884074 ID=MsG0280011050.01.T01:exon:20859;Parent=MsG0280011050.01.T01
Chr2 CDS 78884074 78884520 78884074 ID=MsG0280011050.01.T01:cds;Parent=MsG0280011050.01.T01
Gene Sequence

>MsG0280011050.01.T01

ATGTGGGGACATGGATCACAATATAGAAAGGGACCAGAATCTCTAAGAGGAACACAACCAACAGCAATGCTAAGACTCCCATGTTATTGTTGTGCACCTGGATGCAAGAACAACATTGATCATCCAAGAGCAAAACCACTCAAAGATTTTAGAACACTTCAAACACATTACAAGAGAAAGCATGGAATAAAGCCATTTATGTGTAGAAAGTGTTGCAAAGCATTTGCAGTAAGAGGTGACTGGAGAACTCACGAGAAAAACTGTGGGAAACTTTGGTATTGTTGTTGTGGGTCCGATTTTAAGCACAAAAGATCACTTAAAGATCATATCAAGGCTTTTGGAAATGGACATAAAGCTTATGGAATTGACGAATGTCTTGATCAAGATCAAGATGATGAACATGGTGGCTCTGAAATTGAGCAAGACAATGAATCTTTTCAGTCATAG

Protein sequence

>MsG0280011050.01.T01

MWGHGSQYRKGPESLRGTQPTAMLRLPCYCCAPGCKNNIDHPRAKPLKDFRTLQTHYKRKHGIKPFMCRKCCKAFAVRGDWRTHEKNCGKLWYCCCGSDFKHKRSLKDHIKAFGNGHKAYGIDECLDQDQDDEHGGSEIEQDNESFQS*