AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280011227.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280011227.01.T01 MTR_2g100270 91.111 90 7 1 1 90 97 185 3.34e-32 110
MsG0280011227.01.T01 MTR_2g100260 62.366 93 28 3 1 88 98 188 9.59e-32 109
MsG0280011227.01.T01 MTR_7g096220 49.333 75 38 0 3 77 102 176 1.03e-22 87.0
MsG0280011227.01.T01 MTR_1g070480 51.948 77 37 0 1 77 93 169 1.64e-20 81.3
MsG0280011227.01.T01 MTR_8g016150 57.746 71 30 0 1 71 96 166 1.41e-17 73.2
MsG0280011227.01.T01 MTR_6g092690 43.750 80 45 0 1 80 98 177 3.07e-16 70.1
MsG0280011227.01.T01 MTR_5g083490 42.308 78 43 1 2 77 114 191 6.02e-14 64.3
MsG0280011227.01.T01 MTR_4g014060 41.975 81 45 1 1 79 121 201 3.48e-13 62.4
MsG0280011227.01.T01 MTR_0562s0010 41.975 81 45 1 1 79 121 201 3.48e-13 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280011227.01.T01 AT1G55190 52.564 78 37 0 4 81 101 178 3.60e-21 82.8
MsG0280011227.01.T01 AT3G13710 47.727 88 41 1 4 86 101 188 6.97e-20 79.3
MsG0280011227.01.T01 AT1G17700 49.315 73 37 0 8 80 105 177 2.23e-19 77.8
MsG0280011227.01.T01 AT1G04260 48.684 76 39 0 3 78 84 159 1.51e-17 73.2
MsG0280011227.01.T01 AT2G38360 39.506 81 47 1 1 79 116 196 4.98e-15 67.4
MsG0280011227.01.T01 AT1G55640 40.260 77 46 0 1 77 100 176 6.06e-15 66.6
MsG0280011227.01.T01 AT3G13720 54.795 73 33 0 4 76 101 173 6.85e-15 66.6
MsG0280011227.01.T01 AT2G40380 42.105 76 42 1 4 77 111 186 2.20e-14 65.5
MsG0280011227.01.T01 AT5G56230 31.169 77 53 0 1 77 98 174 5.07e-14 64.3
MsG0280011227.01.T01 AT3G56110 39.744 78 45 1 4 79 109 186 4.93e-13 62.0
MsG0280011227.01.T01 AT3G56110 39.744 78 45 1 4 79 109 186 4.93e-13 62.0
MsG0280011227.01.T01 AT3G56110 39.744 78 45 1 4 79 109 186 4.93e-13 62.0
MsG0280011227.01.T01 AT3G56110 39.744 78 45 1 4 79 109 186 4.93e-13 62.0
MsG0280011227.01.T01 AT5G07110 43.421 76 41 1 4 77 109 184 6.50e-13 61.6
MsG0280011227.01.T01 AT5G01640 35.366 82 51 1 2 81 117 198 2.43e-12 60.5
MsG0280011227.01.T01 AT5G05380 43.590 78 42 1 2 77 110 187 3.60e-11 57.0
MsG0280011227.01.T01 AT1G08770 42.857 77 42 1 3 77 111 187 4.20e-11 57.0
MsG0280011227.01.T01 AT5G05380 43.590 78 42 1 2 77 153 230 4.45e-11 57.4
MsG0280011227.01.T01 AT5G05380 43.590 78 42 1 2 77 161 238 4.48e-11 57.4
MsG0280011227.01.T01 AT5G05380 43.590 78 42 1 2 77 177 254 5.18e-11 57.4
MsG0280011227.01.T01 AT4G29658 42.857 77 43 1 3 78 98 174 9.03e-11 55.8
MsG0280011227.01.T01 AT4G29658 41.772 79 45 1 1 78 33 111 9.42e-11 54.3

Find 14 sgRNAs with CRISPR-Local

Find 14 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGAACCTCTTGTTATCTT+TGG 0.288928 2:+81179696 MsG0280011227.01.T01:CDS
ATTCTTATCGCCGTTGCGGT+TGG 0.459068 2:+81179804 MsG0280011227.01.T01:CDS
TTTCTTGATGAAGAAGAAGA+AGG 0.460660 2:+81179876 MsG0280011227.01.T01:CDS
CGCCGTTGCGGTTGGTGTTG+TGG 0.480076 2:+81179812 MsG0280011227.01.T01:CDS
GAATAACACGATCACTGATA+AGG 0.500904 2:-81179722 None:intergenic
CTTATGTTGATTCTCACCGT+TGG 0.515006 2:+81179750 MsG0280011227.01.T01:CDS
TTCATCAAGAAACAGATCAC+TGG 0.548790 2:-81179865 None:intergenic
AACCACAACACCAACCGCAA+CGG 0.551906 2:-81179814 None:intergenic
GATCAAGAAGCAGAAGCACC+AGG 0.567570 2:-81179915 None:intergenic
CAATATTCTTATCGCCGTTG+CGG 0.580821 2:+81179800 MsG0280011227.01.T01:CDS
AGGCGACCAAAGATAACAAG+AGG 0.631850 2:-81179702 None:intergenic
AGCAAGCAAAAGCAATCCAA+CGG 0.634952 2:-81179766 None:intergenic
TCGTCGTTCTCATGGCTGCG+TGG 0.645538 2:+81179652 None:intergenic
CAAGAAGCAGAAGCACCAGG+TGG 0.685551 2:-81179912 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TTTCTTGATGAAGAAGAAGA+AGG + Chr2:81179876-81179895 MsG0280011227.01.T01:CDS 30.0%
GAATAACACGATCACTGATA+AGG - Chr2:81179725-81179744 None:intergenic 35.0%
TTCATCAAGAAACAGATCAC+TGG - Chr2:81179868-81179887 None:intergenic 35.0%
! GATGAACCTCTTGTTATCTT+TGG + Chr2:81179696-81179715 MsG0280011227.01.T01:CDS 35.0%
AGCAAGCAAAAGCAATCCAA+CGG - Chr2:81179769-81179788 None:intergenic 40.0%
CAATATTCTTATCGCCGTTG+CGG + Chr2:81179800-81179819 MsG0280011227.01.T01:CDS 40.0%
!! CTTATGTTGATTCTCACCGT+TGG + Chr2:81179750-81179769 MsG0280011227.01.T01:CDS 40.0%
AGGCGACCAAAGATAACAAG+AGG - Chr2:81179705-81179724 None:intergenic 45.0%
!! GTTGGATTGCTTTTGCTTGC+TGG + Chr2:81179768-81179787 MsG0280011227.01.T01:CDS 45.0%
AACCACAACACCAACCGCAA+CGG - Chr2:81179817-81179836 None:intergenic 50.0%
ATTCTTATCGCCGTTGCGGT+TGG + Chr2:81179804-81179823 MsG0280011227.01.T01:CDS 50.0%
! GGACTTTCGTTTTCTCCACC+TGG + Chr2:81179897-81179916 MsG0280011227.01.T01:CDS 50.0%
!! CAAGAAGCAGAAGCACCAGG+TGG - Chr2:81179915-81179934 None:intergenic 55.0%
!! CGCCGTTGCGGTTGGTGTTG+TGG + Chr2:81179812-81179831 MsG0280011227.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr2 gene 81179663 81179935 81179663 ID=MsG0280011227.01;Name=MsG0280011227.01
Chr2 mRNA 81179663 81179935 81179663 ID=MsG0280011227.01.T01;Parent=MsG0280011227.01;Name=MsG0280011227.01.T01;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|1|1|0|90
Chr2 exon 81179663 81179935 81179663 ID=MsG0280011227.01.T01:exon:12054;Parent=MsG0280011227.01.T01
Chr2 CDS 81179663 81179935 81179663 ID=MsG0280011227.01.T01:cds;Parent=MsG0280011227.01.T01
Gene Sequence

>MsG0280011227.01.T01

ATGGCTGCGTGGTTGTTTCTCTACTTTCTTCGTGATGAACCTCTTGTTATCTTTGGTCGCCTTATCAGTGATCGTGTTATTCTTGTGCTTATGTTGATTCTCACCGTTGGATTGCTTTTGCTTGCTGGTGCGATTCTCAATATTCTTATCGCCGTTGCGGTTGGTGTTGTGGTTGTTCTTCTTCATGCTGCTTTTAGAAACACCAGTGATCTGTTTCTTGATGAAGAAGAAGAAGGACTTTCGTTTTCTCCACCTGGTGCTTCTGCTTCTTGA
Protein sequence

>MsG0280011227.01.T01

MAAWLFLYFLRDEPLVIFGRLISDRVILVLMLILTVGLLLLAGAILNILIAVAVGVVVVLLHAAFRNTSDLFLDEEEEGLSFSPPGASAS*