Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280011226.01.T01 | KYP58364.1 | 56.25 | 64 | 28 | 0 | 1 | 64 | 109 | 172 | 1.25E-12 | 69.7 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280011226.01.T01 | A0A151SU53 | 56.250 | 64 | 28 | 0 | 1 | 64 | 109 | 172 | 5.95e-13 | 69.7 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180004302.01 | MsG0280011226.01 | -0.807634 | 4.593962e-50 | 2.571775e-47 |
| MsG0280011226.01 | MsG0280011227.01 | 0.905643 | 3.456773e-80 | 5.641331e-76 |
| MsG0280011226.01 | MsG0280011449.01 | -0.819164 | 1.341163e-52 | 1.024180e-49 |
| MsG0280011226.01 | MsG0380012444.01 | -0.812950 | 3.274677e-51 | 2.110309e-48 |
| MsG0280011226.01 | MsG0380016261.01 | 0.807899 | 4.034177e-50 | 2.274016e-47 |
| MsG0280011226.01 | MsG0480023102.01 | 0.827575 | 1.450162e-54 | 1.405464e-51 |
| MsG0280011226.01 | MsG0480023693.01 | 0.802293 | 6.009089e-49 | 2.932806e-46 |
| MsG0280011226.01 | MsG0580024044.01 | 0.801842 | 7.439618e-49 | 3.589473e-46 |
| MsG0280011226.01 | MsG0680030399.01 | -0.821632 | 3.641729e-53 | 2.978838e-50 |
| MsG0280011226.01 | MsG0680034316.01 | 0.804073 | 2.573484e-49 | 1.314559e-46 |
| MsG0280011226.01 | MsG0880042895.01 | -0.813255 | 2.808261e-51 | 1.824685e-48 |
| MsG0280006668.01 | MsG0280011226.01 | 0.808947 | 2.411325e-50 | 1.397234e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 31 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCCGGATTTGAGGCGGTCTT+TGG | 0.291200 | 2:-81179445 | MsG0280011226.01.T01:CDS |
| GGATGATGGCGTAGTTCATC+TGG | 0.387307 | 2:-81179575 | MsG0280011226.01.T01:CDS |
| GCCAAAGACCGCCTCAAATC+CGG | 0.414007 | 2:+81179444 | None:intergenic |
| TCCTCTTCCTCAGTCTTCTC+TGG | 0.414218 | 2:+81179607 | None:intergenic |
| GGAAATAGCGTCGTGGAAGT+TGG | 0.421104 | 2:-81179526 | MsG0280011226.01.T01:CDS |
| ATGAATTCGAGGTTTGTTGC+AGG | 0.451398 | 2:-81179414 | MsG0280011226.01.T01:CDS |
| GACCGCCTCAAATCCGGCCT+CGG | 0.475536 | 2:+81179450 | None:intergenic |
| CATCTGGAAGAAAGAGATGT+TGG | 0.482307 | 2:-81179559 | MsG0280011226.01.T01:CDS |
| GCCTCGGAACTCGCCGTCCA+TGG | 0.485626 | 2:+81179466 | None:intergenic |
| GCCAGAGAAGACTGAGGAAG+AGG | 0.489667 | 2:-81179608 | MsG0280011226.01.T01:CDS |
| TGAGGAAGAGGATGATGAGA+AGG | 0.491579 | 2:-81179596 | MsG0280011226.01.T01:CDS |
| TGAAGGAACGGAGGTTGAAG+AGG | 0.492601 | 2:-81179494 | MsG0280011226.01.T01:CDS |
| TTCGAGGTTTGTTGCAGGTG+GGG | 0.512536 | 2:-81179409 | MsG0280011226.01.T01:CDS |
| GAGTTCCGAGGCCGGATTTG+AGG | 0.519741 | 2:-81179455 | MsG0280011226.01.T01:CDS |
| TGGCGCGTGTGATGAATTCG+AGG | 0.527660 | 2:-81179425 | MsG0280011226.01.T01:CDS |
| AATTCGAGGTTTGTTGCAGG+TGG | 0.530722 | 2:-81179411 | MsG0280011226.01.T01:CDS |
| GAGGATGATGAGAAGGATGA+TGG | 0.534139 | 2:-81179589 | MsG0280011226.01.T01:CDS |
| GAAGTTGGATGGGAGGTTGA+AGG | 0.541239 | 2:-81179511 | MsG0280011226.01.T01:CDS |
| ATAGCGTCGTGGAAGTTGGA+TGG | 0.557067 | 2:-81179522 | MsG0280011226.01.T01:CDS |
| TGGACGGCGAGTTCCGAGGC+CGG | 0.568393 | 2:-81179463 | MsG0280011226.01.T01:CDS |
| TAGCGTCGTGGAAGTTGGAT+GGG | 0.573483 | 2:-81179521 | MsG0280011226.01.T01:CDS |
| ATTCGAGGTTTGTTGCAGGT+GGG | 0.581531 | 2:-81179410 | MsG0280011226.01.T01:CDS |
| TGGATGGGAGGTTGAAGGAA+CGG | 0.585117 | 2:-81179506 | MsG0280011226.01.T01:CDS |
| TGAAGAGGAGTTTCCATGGA+CGG | 0.586287 | 2:-81179479 | MsG0280011226.01.T01:CDS |
| TTCCGAGGCCGGATTTGAGG+CGG | 0.588969 | 2:-81179452 | MsG0280011226.01.T01:CDS |
| AGGTTGAAGAGGAGTTTCCA+TGG | 0.595913 | 2:-81179483 | MsG0280011226.01.T01:CDS |
| TCCATGGACGGCGAGTTCCG+AGG | 0.598894 | 2:-81179467 | MsG0280011226.01.T01:CDS |
| CGTCGTGGAAGTTGGATGGG+AGG | 0.636134 | 2:-81179518 | MsG0280011226.01.T01:CDS |
| ATGGGAGGTTGAAGGAACGG+AGG | 0.651337 | 2:-81179503 | MsG0280011226.01.T01:CDS |
| TTGGATGCCAGAGAAGACTG+AGG | 0.685064 | 2:-81179614 | None:intergenic |
| TGATACGGGAAATAGCGTCG+TGG | 0.762317 | 2:-81179533 | MsG0280011226.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAGAGATGTTGGTTTTGATA+CGG | - | Chr2:81179467-81179486 | MsG0280011226.01.T01:CDS | 30.0% |
| !!! | AGAGATGTTGGTTTTGATAC+GGG | - | Chr2:81179468-81179487 | MsG0280011226.01.T01:CDS | 35.0% |
| CATCTGGAAGAAAGAGATGT+TGG | - | Chr2:81179456-81179475 | MsG0280011226.01.T01:CDS | 40.0% | |
| !! | ATGAATTCGAGGTTTGTTGC+AGG | - | Chr2:81179601-81179620 | MsG0280011226.01.T01:CDS | 40.0% |
| AGGTTGAAGAGGAGTTTCCA+TGG | - | Chr2:81179532-81179551 | MsG0280011226.01.T01:CDS | 45.0% | |
| GAGGATGATGAGAAGGATGA+TGG | - | Chr2:81179426-81179445 | MsG0280011226.01.T01:CDS | 45.0% | |
| TGAAGAGGAGTTTCCATGGA+CGG | - | Chr2:81179536-81179555 | MsG0280011226.01.T01:CDS | 45.0% | |
| TGAGGAAGAGGATGATGAGA+AGG | - | Chr2:81179419-81179438 | MsG0280011226.01.T01:CDS | 45.0% | |
| !! | AATTCGAGGTTTGTTGCAGG+TGG | - | Chr2:81179604-81179623 | MsG0280011226.01.T01:CDS | 45.0% |
| !! | ATTCGAGGTTTGTTGCAGGT+GGG | - | Chr2:81179605-81179624 | MsG0280011226.01.T01:CDS | 45.0% |
| ATAGCGTCGTGGAAGTTGGA+TGG | - | Chr2:81179493-81179512 | MsG0280011226.01.T01:CDS | 50.0% | |
| GAAGTTGGATGGGAGGTTGA+AGG | - | Chr2:81179504-81179523 | MsG0280011226.01.T01:CDS | 50.0% | |
| GGAAATAGCGTCGTGGAAGT+TGG | - | Chr2:81179489-81179508 | MsG0280011226.01.T01:CDS | 50.0% | |
| TAGCGTCGTGGAAGTTGGAT+GGG | - | Chr2:81179494-81179513 | MsG0280011226.01.T01:CDS | 50.0% | |
| TCCTCTTCCTCAGTCTTCTC+TGG | + | Chr2:81179411-81179430 | None:intergenic | 50.0% | |
| TGAAGGAACGGAGGTTGAAG+AGG | - | Chr2:81179521-81179540 | MsG0280011226.01.T01:CDS | 50.0% | |
| TGATACGGGAAATAGCGTCG+TGG | - | Chr2:81179482-81179501 | MsG0280011226.01.T01:CDS | 50.0% | |
| TGGATGGGAGGTTGAAGGAA+CGG | - | Chr2:81179509-81179528 | MsG0280011226.01.T01:CDS | 50.0% | |
| ! | GGATGATGGCGTAGTTCATC+TGG | - | Chr2:81179440-81179459 | MsG0280011226.01.T01:CDS | 50.0% |
| !! | TTCGAGGTTTGTTGCAGGTG+GGG | - | Chr2:81179606-81179625 | MsG0280011226.01.T01:CDS | 50.0% |
| ATGGGAGGTTGAAGGAACGG+AGG | - | Chr2:81179512-81179531 | MsG0280011226.01.T01:CDS | 55.0% | |
| GCCAAAGACCGCCTCAAATC+CGG | + | Chr2:81179574-81179593 | None:intergenic | 55.0% | |
| GCCAGAGAAGACTGAGGAAG+AGG | - | Chr2:81179407-81179426 | MsG0280011226.01.T01:CDS | 55.0% | |
| TGGCGCGTGTGATGAATTCG+AGG | - | Chr2:81179590-81179609 | MsG0280011226.01.T01:CDS | 55.0% | |
| CGTCGTGGAAGTTGGATGGG+AGG | - | Chr2:81179497-81179516 | MsG0280011226.01.T01:CDS | 60.0% | |
| GAGTTCCGAGGCCGGATTTG+AGG | - | Chr2:81179560-81179579 | MsG0280011226.01.T01:CDS | 60.0% | |
| TTCCGAGGCCGGATTTGAGG+CGG | - | Chr2:81179563-81179582 | MsG0280011226.01.T01:CDS | 60.0% | |
| ! | GCCGGATTTGAGGCGGTCTT+TGG | - | Chr2:81179570-81179589 | MsG0280011226.01.T01:CDS | 60.0% |
| GACCGCCTCAAATCCGGCCT+CGG | + | Chr2:81179568-81179587 | None:intergenic | 65.0% | |
| TCCATGGACGGCGAGTTCCG+AGG | - | Chr2:81179548-81179567 | MsG0280011226.01.T01:CDS | 65.0% | |
| GCCTCGGAACTCGCCGTCCA+TGG | + | Chr2:81179552-81179571 | None:intergenic | 70.0% | |
| TGGACGGCGAGTTCCGAGGC+CGG | - | Chr2:81179552-81179571 | MsG0280011226.01.T01:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 81179405 | 81179632 | 81179405 | ID=MsG0280011226.01;Name=MsG0280011226.01 |
| Chr2 | mRNA | 81179405 | 81179632 | 81179405 | ID=MsG0280011226.01.T01;Parent=MsG0280011226.01;Name=MsG0280011226.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|75 |
| Chr2 | exon | 81179405 | 81179632 | 81179405 | ID=MsG0280011226.01.T01:exon:12106;Parent=MsG0280011226.01.T01 |
| Chr2 | CDS | 81179405 | 81179632 | 81179405 | ID=MsG0280011226.01.T01:cds;Parent=MsG0280011226.01.T01 |
| Gene Sequence |
| Protein sequence |
