Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380011687.01.T01 | XP_003598232.1 | 94.273 | 227 | 13 | 0 | 31 | 257 | 1 | 227 | 1.28E-151 | 432 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380011687.01.T01 | Q9SB38 | 32.547 | 212 | 125 | 4 | 44 | 254 | 1 | 195 | 2.51E-34 | 125 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380011687.01.T01 | G7IYI6 | 94.273 | 227 | 13 | 0 | 31 | 257 | 1 | 227 | 6.09e-152 | 432 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0380011687.01 | MsG0880046152.01 | PPI |
| MsG0380011687.01 | MsG0580024488.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380011687.01.T01 | MTR_3g008760 | 94.273 | 227 | 13 | 0 | 31 | 257 | 1 | 227 | 1.54e-155 | 432 |
| MsG0380011687.01.T01 | MTR_4g035855 | 41.463 | 164 | 91 | 2 | 96 | 257 | 43 | 203 | 5.58e-35 | 124 |
| MsG0380011687.01.T01 | MTR_8g104765 | 34.595 | 185 | 112 | 2 | 78 | 254 | 26 | 209 | 9.52e-32 | 116 |
| MsG0380011687.01.T01 | MTR_4g035905 | 37.342 | 158 | 96 | 2 | 96 | 253 | 36 | 190 | 1.78e-31 | 115 |
| MsG0380011687.01.T01 | MTR_3g008840 | 37.791 | 172 | 98 | 4 | 85 | 254 | 18 | 182 | 2.38e-31 | 114 |
| MsG0380011687.01.T01 | MTR_4g120825 | 33.871 | 186 | 114 | 2 | 77 | 254 | 16 | 200 | 3.18e-31 | 115 |
| MsG0380011687.01.T01 | MTR_4g120830 | 33.871 | 186 | 114 | 2 | 77 | 254 | 16 | 200 | 4.33e-31 | 114 |
| MsG0380011687.01.T01 | MTR_4g035880 | 36.709 | 158 | 97 | 2 | 96 | 253 | 36 | 190 | 1.25e-30 | 113 |
| MsG0380011687.01.T01 | MTR_4g035870 | 36.709 | 158 | 97 | 2 | 96 | 253 | 36 | 190 | 4.68e-30 | 111 |
| MsG0380011687.01.T01 | MTR_3g008820 | 36.842 | 171 | 101 | 3 | 85 | 254 | 17 | 181 | 2.03e-29 | 109 |
| MsG0380011687.01.T01 | MTR_5g012000 | 33.540 | 161 | 103 | 2 | 96 | 254 | 48 | 206 | 1.04e-26 | 103 |
| MsG0380011687.01.T01 | MTR_4g101760 | 29.224 | 219 | 125 | 5 | 42 | 254 | 3 | 197 | 3.02e-23 | 94.0 |
| MsG0380011687.01.T01 | MTR_2g043560 | 33.149 | 181 | 97 | 7 | 80 | 253 | 21 | 184 | 6.78e-21 | 87.8 |
| MsG0380011687.01.T01 | MTR_4g124855 | 27.746 | 173 | 120 | 3 | 84 | 253 | 24 | 194 | 1.04e-18 | 82.0 |
| MsG0380011687.01.T01 | MTR_4g025590 | 31.737 | 167 | 96 | 5 | 95 | 249 | 2 | 162 | 1.30e-17 | 78.6 |
| MsG0380011687.01.T01 | MTR_4g025550 | 31.351 | 185 | 105 | 6 | 77 | 249 | 17 | 191 | 1.31e-17 | 79.3 |
| MsG0380011687.01.T01 | MTR_4g025610 | 31.138 | 167 | 97 | 5 | 95 | 249 | 31 | 191 | 1.39e-16 | 76.6 |
| MsG0380011687.01.T01 | MTR_7g108790 | 26.816 | 179 | 120 | 4 | 75 | 253 | 18 | 185 | 1.69e-15 | 73.2 |
| MsG0380011687.01.T01 | MTR_4g025620 | 29.121 | 182 | 107 | 6 | 80 | 249 | 20 | 191 | 2.13e-14 | 70.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380011687.01.T01 | AT5G51520 | 38.265 | 196 | 104 | 3 | 70 | 257 | 18 | 204 | 3.46e-40 | 138 |
| MsG0380011687.01.T01 | AT4G25250 | 32.547 | 212 | 125 | 4 | 44 | 254 | 1 | 195 | 2.56e-35 | 125 |
| MsG0380011687.01.T01 | AT1G62760 | 43.931 | 173 | 83 | 6 | 85 | 253 | 140 | 302 | 3.66e-33 | 122 |
| MsG0380011687.01.T01 | AT4G25260 | 37.654 | 162 | 96 | 2 | 96 | 254 | 37 | 196 | 6.85e-33 | 119 |
| MsG0380011687.01.T01 | AT2G01610 | 34.615 | 182 | 103 | 4 | 86 | 253 | 35 | 214 | 1.66e-32 | 119 |
| MsG0380011687.01.T01 | AT5G62360 | 35.849 | 159 | 98 | 2 | 96 | 254 | 43 | 197 | 5.30e-30 | 112 |
| MsG0380011687.01.T01 | AT1G14890 | 34.483 | 174 | 102 | 3 | 84 | 253 | 47 | 212 | 1.51e-29 | 111 |
| MsG0380011687.01.T01 | AT3G47380 | 32.778 | 180 | 116 | 2 | 77 | 253 | 19 | 196 | 2.62e-29 | 110 |
| MsG0380011687.01.T01 | AT5G62350 | 33.540 | 161 | 102 | 2 | 96 | 253 | 38 | 196 | 3.35e-29 | 109 |
| MsG0380011687.01.T01 | AT1G23205 | 36.095 | 169 | 99 | 3 | 96 | 256 | 33 | 200 | 5.10e-27 | 103 |
| MsG0380011687.01.T01 | AT1G70720 | 34.483 | 174 | 98 | 4 | 84 | 253 | 30 | 191 | 1.60e-25 | 100 |
| MsG0380011687.01.T01 | AT1G70720 | 34.483 | 174 | 98 | 4 | 84 | 253 | 42 | 203 | 1.70e-25 | 100 |
| MsG0380011687.01.T01 | AT1G62770 | 32.184 | 174 | 113 | 3 | 85 | 254 | 22 | 194 | 1.91e-24 | 97.4 |
| MsG0380011687.01.T01 | AT5G20740 | 27.679 | 224 | 142 | 7 | 38 | 254 | 16 | 226 | 8.48e-23 | 94.0 |
| MsG0380011687.01.T01 | AT5G20740 | 29.762 | 168 | 111 | 4 | 91 | 254 | 28 | 192 | 1.40e-22 | 92.4 |
| MsG0380011687.01.T01 | AT4G12390 | 34.969 | 163 | 100 | 3 | 96 | 254 | 39 | 199 | 3.13e-20 | 86.3 |
| MsG0380011687.01.T01 | AT4G00080 | 32.597 | 181 | 109 | 5 | 83 | 254 | 22 | 198 | 1.85e-19 | 84.3 |
| MsG0380011687.01.T01 | AT3G62820 | 32.530 | 166 | 97 | 5 | 94 | 253 | 31 | 187 | 5.77e-19 | 82.4 |
| MsG0380011687.01.T01 | AT2G47670 | 33.140 | 172 | 99 | 6 | 89 | 252 | 37 | 200 | 2.35e-17 | 78.6 |
| MsG0380011687.01.T01 | AT3G14300 | 28.916 | 166 | 103 | 4 | 97 | 252 | 271 | 431 | 3.43e-11 | 63.5 |
Find 33 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCGTGTTTCGTGATTTAT+AGG | 0.265201 | 3:-3513482 | None:intergenic |
| TGGCTTCCATGTTTAGATCT+TGG | 0.323087 | 3:-3513549 | None:intergenic |
| TCTTGATGCCATGGCACATT+TGG | 0.327434 | 3:+3514020 | MsG0380011687.01.T01:CDS |
| AAAGATTTGTAACATATATA+TGG | 0.334992 | 3:-3513790 | None:intergenic |
| AATGATCAAACTTGCTATGA+TGG | 0.416303 | 3:+3514108 | MsG0380011687.01.T01:CDS |
| GTTGTTTAGAAGCTTTGATA+TGG | 0.428821 | 3:-3513908 | None:intergenic |
| GACAATAAATTGTCAAGCAT+TGG | 0.458680 | 3:+3513934 | MsG0380011687.01.T01:CDS |
| ATATGAAGTGTACGAAAAGT+TGG | 0.475164 | 3:-3513695 | None:intergenic |
| TCTGATCTTGTCCCCAAGTG+TGG | 0.483968 | 3:-3514154 | None:intergenic |
| GAAGTTGTGCAAGATTGCTT+TGG | 0.484978 | 3:+3513961 | MsG0380011687.01.T01:CDS |
| TTTGAAATCATTGGTGTTGG+TGG | 0.488377 | 3:-3513731 | None:intergenic |
| ATGAATGTGGATTCCACACT+TGG | 0.503396 | 3:+3514141 | MsG0380011687.01.T01:CDS |
| ATTGGTGTTGGTGGAATTTG+AGG | 0.506534 | 3:-3513722 | None:intergenic |
| AGATTAGTAACATAAAGACT+TGG | 0.517077 | 3:+3514067 | MsG0380011687.01.T01:CDS |
| AGTGTACGAAAAGTTGGTTT+TGG | 0.528393 | 3:-3513689 | None:intergenic |
| GCAAGATTCTCTTGATGCCA+TGG | 0.535597 | 3:+3514011 | MsG0380011687.01.T01:CDS |
| AAACACGAAGTCATTTAGAA+AGG | 0.540934 | 3:+3513495 | MsG0380011687.01.T01:CDS |
| GCAAGTTTGATCATTAGTAA+TGG | 0.544323 | 3:-3514100 | None:intergenic |
| ATGATCAAACTTGCTATGAT+GGG | 0.546835 | 3:+3514109 | MsG0380011687.01.T01:CDS |
| GTAACATATATATGGATATG+TGG | 0.547384 | 3:-3513782 | None:intergenic |
| TTGGTGTTGGTGGAATTTGA+GGG | 0.548839 | 3:-3513721 | None:intergenic |
| GATTAGTAACATAAAGACTT+GGG | 0.551030 | 3:+3514068 | MsG0380011687.01.T01:CDS |
| TAGCATCACTTCAGCATGTG+AGG | 0.581960 | 3:+3513639 | MsG0380011687.01.T01:CDS |
| TAGAAGCTTTGATATGGCGG+AGG | 0.586848 | 3:-3513902 | None:intergenic |
| GTTTAGAAGCTTTGATATGG+CGG | 0.591317 | 3:-3513905 | None:intergenic |
| TGAATGTGGATTCCACACTT+GGG | 0.595762 | 3:+3514142 | MsG0380011687.01.T01:CDS |
| ATCAGCATCCAAATGTGCCA+TGG | 0.600401 | 3:-3514028 | None:intergenic |
| ATTATGCAACACTTCACTCT+CGG | 0.609606 | 3:+3513852 | MsG0380011687.01.T01:CDS |
| GAATGTGGATTCCACACTTG+GGG | 0.621754 | 3:+3514143 | MsG0380011687.01.T01:CDS |
| AGATTGCTTTGGCAATGTGA+AGG | 0.623852 | 3:+3513972 | MsG0380011687.01.T01:CDS |
| TGGGTTTGATGATATGAATG+TGG | 0.629419 | 3:+3514128 | MsG0380011687.01.T01:CDS |
| GAAGTGCCAAGATCTAAACA+TGG | 0.689460 | 3:+3513543 | MsG0380011687.01.T01:CDS |
| ACTCTCGGTCGCATTAAATG+CGG | 0.692627 | 3:+3513867 | MsG0380011687.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAGATTTGTAACATATATA+TGG | - | Chr3:3513793-3513812 | None:intergenic | 15.0% |
| !!! | ATTGCATGAATTTTTAATAT+AGG | - | Chr3:3513758-3513777 | None:intergenic | 15.0% |
| !!! | AATATAGGTTTTGAAATCAT+TGG | - | Chr3:3513743-3513762 | None:intergenic | 20.0% |
| ! | AGATTAGTAACATAAAGACT+TGG | + | Chr3:3514067-3514086 | MsG0380011687.01.T01:CDS | 25.0% |
| ! | GATTAGTAACATAAAGACTT+GGG | + | Chr3:3514068-3514087 | MsG0380011687.01.T01:CDS | 25.0% |
| ! | GTAACATATATATGGATATG+TGG | - | Chr3:3513785-3513804 | None:intergenic | 25.0% |
| AAACACGAAGTCATTTAGAA+AGG | + | Chr3:3513495-3513514 | MsG0380011687.01.T01:CDS | 30.0% | |
| ATATGAAGTGTACGAAAAGT+TGG | - | Chr3:3513698-3513717 | None:intergenic | 30.0% | |
| GACAATAAATTGTCAAGCAT+TGG | + | Chr3:3513934-3513953 | MsG0380011687.01.T01:CDS | 30.0% | |
| GCAAGTTTGATCATTAGTAA+TGG | - | Chr3:3514103-3514122 | None:intergenic | 30.0% | |
| ! | AGAAAAACTTGCAAAAGTGT+TGG | - | Chr3:3513572-3513591 | None:intergenic | 30.0% |
| ! | GTTGTTTAGAAGCTTTGATA+TGG | - | Chr3:3513911-3513930 | None:intergenic | 30.0% |
| !! | AATGATCAAACTTGCTATGA+TGG | + | Chr3:3514108-3514127 | MsG0380011687.01.T01:CDS | 30.0% |
| !! | ATGATCAAACTTGCTATGAT+GGG | + | Chr3:3514109-3514128 | MsG0380011687.01.T01:CDS | 30.0% |
| !!! | GAAGTGTTGCATAATTTTAG+AGG | - | Chr3:3513847-3513866 | None:intergenic | 30.0% |
| ATTATGCAACACTTCACTCT+CGG | + | Chr3:3513852-3513871 | MsG0380011687.01.T01:CDS | 35.0% | |
| CTTCGTGTTTCGTGATTTAT+AGG | - | Chr3:3513485-3513504 | None:intergenic | 35.0% | |
| TCCCTATGCTTCAAAAATTG+AGG | + | Chr3:3513816-3513835 | MsG0380011687.01.T01:CDS | 35.0% | |
| ! | GTTTAGAAGCTTTGATATGG+CGG | - | Chr3:3513908-3513927 | None:intergenic | 35.0% |
| ! | TGGGTTTGATGATATGAATG+TGG | + | Chr3:3514128-3514147 | MsG0380011687.01.T01:CDS | 35.0% |
| !! | AGTGTACGAAAAGTTGGTTT+TGG | - | Chr3:3513692-3513711 | None:intergenic | 35.0% |
| !! | TTTGAAATCATTGGTGTTGG+TGG | - | Chr3:3513734-3513753 | None:intergenic | 35.0% |
| !!! | AAAGTTGGTTTTGGTGGAAT+TGG | - | Chr3:3513683-3513702 | None:intergenic | 35.0% |
| !!! | CAGAAGAAGAGTTTTGAATG+TGG | + | Chr3:3514173-3514192 | MsG0380011687.01.T01:CDS | 35.0% |
| !!! | GCCTCAATTTTTGAAGCATA+GGG | - | Chr3:3513820-3513839 | None:intergenic | 35.0% |
| !!! | GGTTTTGAAATCATTGGTGT+TGG | - | Chr3:3513737-3513756 | None:intergenic | 35.0% |
| !!! | TGCCTCAATTTTTGAAGCAT+AGG | - | Chr3:3513821-3513840 | None:intergenic | 35.0% |
| AGATTGCTTTGGCAATGTGA+AGG | + | Chr3:3513972-3513991 | MsG0380011687.01.T01:CDS | 40.0% | |
| ATGAATGTGGATTCCACACT+TGG | + | Chr3:3514141-3514160 | MsG0380011687.01.T01:CDS | 40.0% | |
| GAAGTTGTGCAAGATTGCTT+TGG | + | Chr3:3513961-3513980 | MsG0380011687.01.T01:CDS | 40.0% | |
| TGAATGTGGATTCCACACTT+GGG | + | Chr3:3514142-3514161 | MsG0380011687.01.T01:CDS | 40.0% | |
| TGGCTTCCATGTTTAGATCT+TGG | - | Chr3:3513552-3513571 | None:intergenic | 40.0% | |
| ! | GAAGTGCCAAGATCTAAACA+TGG | + | Chr3:3513543-3513562 | MsG0380011687.01.T01:CDS | 40.0% |
| !! | ATTGGTGTTGGTGGAATTTG+AGG | - | Chr3:3513725-3513744 | None:intergenic | 40.0% |
| !! | TTGGTGTTGGTGGAATTTGA+GGG | - | Chr3:3513724-3513743 | None:intergenic | 40.0% |
| !!! | GAAGAGTTTTGAATGTGGCA+AGG | + | Chr3:3514178-3514197 | MsG0380011687.01.T01:CDS | 40.0% |
| !!! | GTACGAAAAGTTGGTTTTGG+TGG | - | Chr3:3513689-3513708 | None:intergenic | 40.0% |
| !!! | TGGTTTTGGTGGAATTGGTA+GGG | - | Chr3:3513678-3513697 | None:intergenic | 40.0% |
| !!! | TTGGTTTTGGTGGAATTGGT+AGG | - | Chr3:3513679-3513698 | None:intergenic | 40.0% |
| ACTCTCGGTCGCATTAAATG+CGG | + | Chr3:3513867-3513886 | MsG0380011687.01.T01:CDS | 45.0% | |
| ATCAGCATCCAAATGTGCCA+TGG | - | Chr3:3514031-3514050 | None:intergenic | 45.0% | |
| GAATGTGGATTCCACACTTG+GGG | + | Chr3:3514143-3514162 | MsG0380011687.01.T01:CDS | 45.0% | |
| GCAAGATTCTCTTGATGCCA+TGG | + | Chr3:3514011-3514030 | MsG0380011687.01.T01:CDS | 45.0% | |
| TAGCATCACTTCAGCATGTG+AGG | + | Chr3:3513639-3513658 | MsG0380011687.01.T01:CDS | 45.0% | |
| TCTTGATGCCATGGCACATT+TGG | + | Chr3:3514020-3514039 | MsG0380011687.01.T01:CDS | 45.0% | |
| ! | TAGAAGCTTTGATATGGCGG+AGG | - | Chr3:3513905-3513924 | None:intergenic | 45.0% |
| TCTGATCTTGTCCCCAAGTG+TGG | - | Chr3:3514157-3514176 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 3513472 | 3514245 | 3513472 | ID=MsG0380011687.01;Name=MsG0380011687.01 |
| Chr3 | mRNA | 3513472 | 3514245 | 3513472 | ID=MsG0380011687.01.T01;Parent=MsG0380011687.01;Name=MsG0380011687.01.T01;_AED=0.06;_eAED=0.06;_QI=0|-1|0|1|-1|1|1|0|257 |
| Chr3 | exon | 3513472 | 3514245 | 3513472 | ID=MsG0380011687.01.T01:exon:1038;Parent=MsG0380011687.01.T01 |
| Chr3 | CDS | 3513472 | 3514245 | 3513472 | ID=MsG0380011687.01.T01:cds;Parent=MsG0380011687.01.T01 |
| Gene Sequence |
| Protein sequence |
