AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580024488.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024488.01.T01 MTR_5g012000 97.826 184 4 0 1 184 34 217 5.81e-134 374
MsG0580024488.01.T01 MTR_4g101760 64.641 181 58 1 5 179 23 203 1.48e-82 243
MsG0580024488.01.T01 MTR_3g008840 53.571 168 77 1 11 178 21 187 8.53e-65 197
MsG0580024488.01.T01 MTR_3g008820 51.190 168 81 1 11 178 20 186 3.36e-62 191
MsG0580024488.01.T01 MTR_4g035905 43.030 165 91 1 15 179 36 197 6.09e-45 147
MsG0580024488.01.T01 MTR_4g035870 42.073 164 92 1 15 178 36 196 7.20e-42 139
MsG0580024488.01.T01 MTR_4g035880 42.073 164 92 1 15 178 36 196 2.06e-41 138
MsG0580024488.01.T01 MTR_4g025550 39.888 178 95 4 15 183 32 206 9.66e-39 132
MsG0580024488.01.T01 MTR_4g025610 39.773 176 98 4 15 183 32 206 1.90e-37 129
MsG0580024488.01.T01 MTR_4g025590 38.636 176 96 4 15 181 3 175 1.02e-36 126
MsG0580024488.01.T01 MTR_4g025620 37.931 174 96 4 15 179 32 202 4.31e-35 122
MsG0580024488.01.T01 MTR_8g104765 38.596 171 96 3 13 175 42 211 1.12e-33 119
MsG0580024488.01.T01 MTR_4g120825 38.596 171 96 3 13 175 33 202 1.24e-33 119
MsG0580024488.01.T01 MTR_4g120830 38.596 171 96 3 13 175 33 202 1.97e-33 118
MsG0580024488.01.T01 MTR_7g108790 35.802 162 99 2 16 177 34 190 2.44e-30 110
MsG0580024488.01.T01 MTR_3g008760 34.161 161 102 2 15 173 66 224 4.30e-28 105
MsG0580024488.01.T01 MTR_2g043560 35.912 181 95 6 4 177 23 189 5.60e-26 99.0
MsG0580024488.01.T01 MTR_4g124855 32.370 173 104 4 12 177 33 199 5.56e-24 94.0
MsG0580024488.01.T01 MTR_4g035855 31.056 161 110 1 15 175 43 202 1.20e-22 90.5
MsG0580024488.01.T01 MTR_1g088540 30.405 148 98 2 30 177 1 143 2.37e-19 80.5
MsG0580024488.01.T01 MTR_3g091380 30.864 162 107 2 12 170 74 233 1.07e-17 80.5
MsG0580024488.01.T01 MTR_1g008140 29.762 168 106 3 12 170 78 242 6.90e-17 78.2
MsG0580024488.01.T01 MTR_8g023310 25.478 157 113 2 16 170 68 222 6.68e-15 72.4
MsG0580024488.01.T01 MTR_8g023150 29.341 167 108 5 8 171 69 228 3.27e-11 61.6
MsG0580024488.01.T01 MTR_2g044810 26.901 171 112 5 8 175 31 191 7.22e-11 60.5
MsG0580024488.01.T01 MTR_2g044810 26.901 171 112 5 8 175 31 191 9.54e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024488.01.T01 AT4G12390 56.805 169 69 2 15 180 39 206 1.13e-60 187
MsG0580024488.01.T01 AT1G62770 53.514 185 77 5 8 184 21 204 1.50e-58 182
MsG0580024488.01.T01 AT4G25260 47.977 173 89 1 7 178 29 201 5.34e-55 173
MsG0580024488.01.T01 AT5G62350 47.273 165 86 1 15 178 38 202 7.64e-55 172
MsG0580024488.01.T01 AT3G47380 46.988 166 87 1 14 178 37 202 1.88e-51 164
MsG0580024488.01.T01 AT5G62360 41.818 165 92 2 14 178 42 202 2.38e-45 149
MsG0580024488.01.T01 AT1G62760 43.353 173 88 2 11 179 143 309 5.62e-42 143
MsG0580024488.01.T01 AT1G14890 41.566 166 90 2 14 177 57 217 6.79e-39 132
MsG0580024488.01.T01 AT2G01610 40.113 177 93 3 14 177 43 219 2.09e-37 129
MsG0580024488.01.T01 AT5G20740 40.361 166 90 3 14 174 32 193 1.18e-35 124
MsG0580024488.01.T01 AT5G20740 40.361 166 90 3 14 174 66 227 4.05e-35 123
MsG0580024488.01.T01 AT4G25250 34.969 163 104 2 15 176 37 198 2.51e-34 120
MsG0580024488.01.T01 AT4G00080 38.788 165 94 4 15 173 35 198 3.24e-33 117
MsG0580024488.01.T01 AT1G70720 34.270 178 108 3 3 175 33 206 4.68e-29 107
MsG0580024488.01.T01 AT1G70720 34.270 178 108 3 3 175 21 194 4.70e-29 107
MsG0580024488.01.T01 AT5G51520 33.929 168 101 2 15 174 37 202 8.23e-28 103
MsG0580024488.01.T01 AT1G23205 35.326 184 98 5 3 172 21 197 6.61e-25 96.3
MsG0580024488.01.T01 AT3G62820 33.537 164 102 4 15 176 33 191 5.41e-22 88.6
MsG0580024488.01.T01 AT3G14310 28.488 172 118 3 9 177 50 219 7.98e-21 89.4
MsG0580024488.01.T01 AT2G47670 30.675 163 109 2 13 175 46 204 5.38e-20 83.6
MsG0580024488.01.T01 AT1G53830 28.235 170 119 2 9 177 55 222 4.47e-18 81.6
MsG0580024488.01.T01 AT3G49220 32.184 174 92 7 12 178 128 282 1.71e-15 73.9
MsG0580024488.01.T01 AT3G49220 32.184 174 92 7 12 178 128 282 3.32e-15 73.2
MsG0580024488.01.T01 AT3G49220 32.184 174 92 7 12 178 83 237 3.80e-15 73.2
MsG0580024488.01.T01 AT5G53370 27.500 160 108 4 12 170 107 259 3.93e-13 67.0
MsG0580024488.01.T01 AT5G53370 27.500 160 108 4 12 170 71 223 5.11e-13 67.0
MsG0580024488.01.T01 AT3G14300 26.136 176 123 2 10 179 265 439 1.87e-12 65.5
MsG0580024488.01.T01 AT5G62340 26.966 178 106 6 15 178 39 206 6.10e-11 59.3

Find 48 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGATAACACTTGTCTTGA+TGG 0.271370 5:+5507586 MsG0580024488.01.T01:CDS
ACTTGTCTTGATGGCTTTGC+TGG 0.284433 5:+5507595 MsG0580024488.01.T01:CDS
AGAAAACATGGGTGACAGTT+TGG 0.352972 5:+5507453 MsG0580024488.01.T01:CDS
ATTGACAACCCTGTCCTTGA+TGG 0.385514 5:-5507647 None:intergenic
ACAGTCTTAGCCAATCGGTT+AGG 0.388918 5:+5507476 MsG0580024488.01.T01:CDS
TACCAGCAACACACTCGCTT+TGG 0.400248 5:+5507681 MsG0580024488.01.T01:CDS
ATGGGGTATGCGAGCGTGAT+TGG 0.403801 5:+5507292 MsG0580024488.01.T01:CDS
AACCAAAGCGAGTGTGTTGC+TGG 0.408904 5:-5507683 None:intergenic
TTCCATGAATGGAATTGTTA+AGG 0.412818 5:+5507621 MsG0580024488.01.T01:CDS
TCGTTAGTTGTCGCTCGCTT+TGG 0.415738 5:-5507315 None:intergenic
AGCCTTAACAATTCCATTCA+TGG 0.416556 5:-5507623 None:intergenic
ACATGAACAACGTGCAGACT+TGG 0.438369 5:+5507545 MsG0580024488.01.T01:CDS
AGCCAATCGGTTAGGGAGTT+AGG 0.439103 5:+5507484 MsG0580024488.01.T01:CDS
TTTGCTGGTCCTTCCATGAA+TGG 0.446590 5:+5507610 MsG0580024488.01.T01:CDS
CGATGCACTCGATCGTGTCC+TGG 0.450154 5:-5507362 None:intergenic
CAGTCTTAGCCAATCGGTTA+GGG 0.452763 5:+5507477 MsG0580024488.01.T01:CDS
AGACATTGAACGCATAAAAC+AGG 0.460054 5:-5507268 None:intergenic
GGCAGTATTGGTCATGCTGT+TGG 0.460878 5:+5507505 MsG0580024488.01.T01:CDS
TTTATGCGTTCAATGTCTCA+TGG 0.462904 5:+5507273 MsG0580024488.01.T01:CDS
GTTAGGGAGTTAGGCAGTAT+TGG 0.470731 5:+5507493 MsG0580024488.01.T01:CDS
GAACTCTGATGGGGTTATAG+AGG 0.476311 5:-5507215 None:intergenic
TTAAGGCTGCCATCAAGGAC+AGG 0.476862 5:+5507638 MsG0580024488.01.T01:CDS
TGTACGGCTCTGTACTCCCT+TGG 0.486585 5:-5507421 None:intergenic
CTCTATCAGTGAGCATATCC+AGG 0.495431 5:+5507344 MsG0580024488.01.T01:CDS
TTATGCGTTCAATGTCTCAT+GGG 0.496994 5:+5507274 MsG0580024488.01.T01:CDS
TTTGGACAGTCTTAGCCAAT+CGG 0.504656 5:+5507471 MsG0580024488.01.T01:CDS
GGCAAGAGGCATCAAGCCAA+GGG 0.526611 5:+5507405 MsG0580024488.01.T01:CDS
TTCTGCAGTTCGATGGCTTG+AGG 0.534233 5:-5507716 None:intergenic
AATTGTTAAGGCTGCCATCA+AGG 0.538854 5:+5507633 MsG0580024488.01.T01:CDS
TGCCTAACTCCCTAACCGAT+TGG 0.544285 5:-5507486 None:intergenic
AGGCAAGAGGCATCAAGCCA+AGG 0.545433 5:+5507404 MsG0580024488.01.T01:CDS
GAGGACTTGATGAACTCTGA+TGG 0.549989 5:-5507226 None:intergenic
AGGACTTGATGAACTCTGAT+GGG 0.550827 5:-5507225 None:intergenic
AAGGAGTTTCTGCAGTTCGA+TGG 0.560457 5:-5507723 None:intergenic
ACAAGACTGCATAGAAAACA+TGG 0.577763 5:+5507441 MsG0580024488.01.T01:CDS
CATGAACAACGTGCAGACTT+GGG 0.585314 5:+5507546 MsG0580024488.01.T01:CDS
TATGCGTTCAATGTCTCATG+GGG 0.593117 5:+5507275 MsG0580024488.01.T01:CDS
TTAACAATTCCATTCATGGA+AGG 0.596367 5:-5507619 None:intergenic
TAAGGCTGCCATCAAGGACA+GGG 0.619720 5:+5507639 MsG0580024488.01.T01:CDS
TATTGGTCATGCTGTTGGAG+AGG 0.620267 5:+5507510 MsG0580024488.01.T01:CDS
TAGAGGATTCTGCAGTCACA+AGG 0.636790 5:-5507198 None:intergenic
CAGAAGATATCAAAGGCAAG+AGG 0.641738 5:+5507391 MsG0580024488.01.T01:CDS
GTTTGTACAGAAGATATCAA+AGG 0.649432 5:+5507384 MsG0580024488.01.T01:CDS
ATAGCGAGTAGCTCTACATG+AGG 0.683240 5:-5507245 None:intergenic
GGATATGCTCACTGATAGAG+CGG 0.692375 5:-5507341 None:intergenic
GGACTTGATGAACTCTGATG+GGG 0.708124 5:-5507224 None:intergenic
CAAGACTGCATAGAAAACAT+GGG 0.713583 5:+5507442 MsG0580024488.01.T01:CDS
CTGTTGGAGAGGACTTCGTG+TGG 0.729717 5:+5507521 MsG0580024488.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
GTTTGTACAGAAGATATCAA+AGG + Chr5:5507384-5507403 MsG0580024488.01.T01:CDS 30.0%
TTAACAATTCCATTCATGGA+AGG - Chr5:5507622-5507641 None:intergenic 30.0%
TTCCATGAATGGAATTGTTA+AGG + Chr5:5507621-5507640 MsG0580024488.01.T01:CDS 30.0%
ACAAGACTGCATAGAAAACA+TGG + Chr5:5507441-5507460 MsG0580024488.01.T01:CDS 35.0%
AGACATTGAACGCATAAAAC+AGG - Chr5:5507271-5507290 None:intergenic 35.0%
AGCCTTAACAATTCCATTCA+TGG - Chr5:5507626-5507645 None:intergenic 35.0%
CAAGACTGCATAGAAAACAT+GGG + Chr5:5507442-5507461 MsG0580024488.01.T01:CDS 35.0%
GATGATAACACTTGTCTTGA+TGG + Chr5:5507586-5507605 MsG0580024488.01.T01:CDS 35.0%
TTATGCGTTCAATGTCTCAT+GGG + Chr5:5507274-5507293 MsG0580024488.01.T01:CDS 35.0%
TTTATGCGTTCAATGTCTCA+TGG + Chr5:5507273-5507292 MsG0580024488.01.T01:CDS 35.0%
!! GTTTTCTATGCAGTCTTGTA+CGG - Chr5:5507440-5507459 None:intergenic 35.0%
AATTGTTAAGGCTGCCATCA+AGG + Chr5:5507633-5507652 MsG0580024488.01.T01:CDS 40.0%
AGAAAACATGGGTGACAGTT+TGG + Chr5:5507453-5507472 MsG0580024488.01.T01:CDS 40.0%
AGGACTTGATGAACTCTGAT+GGG - Chr5:5507228-5507247 None:intergenic 40.0%
CAGAAGATATCAAAGGCAAG+AGG + Chr5:5507391-5507410 MsG0580024488.01.T01:CDS 40.0%
TATGCGTTCAATGTCTCATG+GGG + Chr5:5507275-5507294 MsG0580024488.01.T01:CDS 40.0%
TTTGGACAGTCTTAGCCAAT+CGG + Chr5:5507471-5507490 MsG0580024488.01.T01:CDS 40.0%
AAGGAGTTTCTGCAGTTCGA+TGG - Chr5:5507726-5507745 None:intergenic 45.0%
ACAGTCTTAGCCAATCGGTT+AGG + Chr5:5507476-5507495 MsG0580024488.01.T01:CDS 45.0%
ACATGAACAACGTGCAGACT+TGG + Chr5:5507545-5507564 MsG0580024488.01.T01:CDS 45.0%
ATTGACAACCCTGTCCTTGA+TGG - Chr5:5507650-5507669 None:intergenic 45.0%
CAGTCTTAGCCAATCGGTTA+GGG + Chr5:5507477-5507496 MsG0580024488.01.T01:CDS 45.0%
CATGAACAACGTGCAGACTT+GGG + Chr5:5507546-5507565 MsG0580024488.01.T01:CDS 45.0%
CTCTATCAGTGAGCATATCC+AGG + Chr5:5507344-5507363 MsG0580024488.01.T01:CDS 45.0%
GAACTCTGATGGGGTTATAG+AGG - Chr5:5507218-5507237 None:intergenic 45.0%
GAGGACTTGATGAACTCTGA+TGG - Chr5:5507229-5507248 None:intergenic 45.0%
GGACTTGATGAACTCTGATG+GGG - Chr5:5507227-5507246 None:intergenic 45.0%
GGATATGCTCACTGATAGAG+CGG - Chr5:5507344-5507363 None:intergenic 45.0%
GTTAGGGAGTTAGGCAGTAT+TGG + Chr5:5507493-5507512 MsG0580024488.01.T01:CDS 45.0%
TAGAGGATTCTGCAGTCACA+AGG - Chr5:5507201-5507220 None:intergenic 45.0%
TATTGGTCATGCTGTTGGAG+AGG + Chr5:5507510-5507529 MsG0580024488.01.T01:CDS 45.0%
TTTGCTGGTCCTTCCATGAA+TGG + Chr5:5507610-5507629 MsG0580024488.01.T01:CDS 45.0%
! ACTTGTCTTGATGGCTTTGC+TGG + Chr5:5507595-5507614 MsG0580024488.01.T01:CDS 45.0%
! ATAGCGAGTAGCTCTACATG+AGG - Chr5:5507248-5507267 None:intergenic 45.0%
AACCAAAGCGAGTGTGTTGC+TGG - Chr5:5507686-5507705 None:intergenic 50.0%
AGCCAATCGGTTAGGGAGTT+AGG + Chr5:5507484-5507503 MsG0580024488.01.T01:CDS 50.0%
TAAGGCTGCCATCAAGGACA+GGG + Chr5:5507639-5507658 MsG0580024488.01.T01:CDS 50.0%
TACCAGCAACACACTCGCTT+TGG + Chr5:5507681-5507700 MsG0580024488.01.T01:CDS 50.0%
TCGTTAGTTGTCGCTCGCTT+TGG - Chr5:5507318-5507337 None:intergenic 50.0%
TGCCTAACTCCCTAACCGAT+TGG - Chr5:5507489-5507508 None:intergenic 50.0%
TTAAGGCTGCCATCAAGGAC+AGG + Chr5:5507638-5507657 MsG0580024488.01.T01:CDS 50.0%
TTCTGCAGTTCGATGGCTTG+AGG - Chr5:5507719-5507738 None:intergenic 50.0%
!! GGCAGTATTGGTCATGCTGT+TGG + Chr5:5507505-5507524 MsG0580024488.01.T01:CDS 50.0%
ATGGGGTATGCGAGCGTGAT+TGG + Chr5:5507292-5507311 MsG0580024488.01.T01:CDS 55.0%
CTGTTGGAGAGGACTTCGTG+TGG + Chr5:5507521-5507540 MsG0580024488.01.T01:CDS 55.0%
TGTACGGCTCTGTACTCCCT+TGG - Chr5:5507424-5507443 None:intergenic 55.0%
!! AGGCAAGAGGCATCAAGCCA+AGG + Chr5:5507404-5507423 MsG0580024488.01.T01:CDS 55.0%
!! GGCAAGAGGCATCAAGCCAA+GGG + Chr5:5507405-5507424 MsG0580024488.01.T01:CDS 55.0%
CGATGCACTCGATCGTGTCC+TGG - Chr5:5507365-5507384 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr5 gene 5507193 5507747 5507193 ID=MsG0580024488.01;Name=MsG0580024488.01
Chr5 mRNA 5507193 5507747 5507193 ID=MsG0580024488.01.T01;Parent=MsG0580024488.01;Name=MsG0580024488.01.T01;_AED=0.44;_eAED=0.44;_QI=0|-1|0|1|-1|1|1|0|184
Chr5 exon 5507193 5507747 5507193 ID=MsG0580024488.01.T01:exon:19081;Parent=MsG0580024488.01.T01
Chr5 CDS 5507193 5507747 5507193 ID=MsG0580024488.01.T01:cds;Parent=MsG0580024488.01.T01
Gene Sequence

>MsG0580024488.01.T01

ATGAGCCTTGTGACTGCAGAATCCTCTATAACCCCATCAGAGTTCATCAAGTCCTCATGTAGAGCTACTCGCTATCCTGTTTTATGCGTTCAATGTCTCATGGGGTATGCGAGCGTGATTGGCCAAAGCGAGCGACAACTAACGATAACCGCTCTATCAGTGAGCATATCCAGGACACGATCGAGTGCATCGTTTGTACAGAAGATATCAAAGGCAAGAGGCATCAAGCCAAGGGAGTACAGAGCCGTACAAGACTGCATAGAAAACATGGGTGACAGTTTGGACAGTCTTAGCCAATCGGTTAGGGAGTTAGGCAGTATTGGTCATGCTGTTGGAGAGGACTTCGTGTGGCACATGAACAACGTGCAGACTTGGGTCAGTGCTGCACTTACTGATGATAACACTTGTCTTGATGGCTTTGCTGGTCCTTCCATGAATGGAATTGTTAAGGCTGCCATCAAGGACAGGGTTGTCAATGTTGCTCAAGTTACCAGCAACACACTCGCTTTGGTTAATCACTTCGCCTCAAGCCATCGAACTGCAGAAACTCCTTAA

Protein sequence

>MsG0580024488.01.T01

MSLVTAESSITPSEFIKSSCRATRYPVLCVQCLMGYASVIGQSERQLTITALSVSISRTRSSASFVQKISKARGIKPREYRAVQDCIENMGDSLDSLSQSVRELGSIGHAVGEDFVWHMNNVQTWVSAALTDDNTCLDGFAGPSMNGIVKAAIKDRVVNVAQVTSNTLALVNHFASSHRTAETP*