AlfalfaGEDB | MsG0580024488.01

M. sativa cultivar ZhongmuNo.1 / MsG0580024488.01

KEGG: -
GO: GO:0004857
PFAM: PF04043

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024488.01.T01	AFK45126.1	97.826	184	4	0	1	184	13	196	4.25E-130	373

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024488.01.T01	P17407	49.102	167	85	0	13	179	26	192	7.12E-60	187

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024488.01.T01	G7KG88	97.826	184	4	0	1	184	34	217	2.29e-130	374
MsG0580024488.01.T01	I3SXY4	97.826	184	4	0	1	184	13	196	2.03e-130	373

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047831.01	MsG0580024488.01	0.847428	1.163438e-59	2.067405e-56
MsG0080047967.01	MsG0580024488.01	-0.829997	3.762822e-55	3.914300e-52
MsG0080048059.01	MsG0580024488.01	-0.823168	1.601565e-53	1.368539e-50
MsG0080048352.01	MsG0580024488.01	0.804815	1.802658e-49	9.385950e-47
MsG0080048694.01	MsG0580024488.01	-0.833745	4.470586e-56	5.195838e-53
MsG0080048695.01	MsG0580024488.01	-0.834031	3.791685e-56	4.445009e-53
MsG0080048813.01	MsG0580024488.01	-0.841954	3.472662e-58	5.185542e-55
MsG0080048889.01	MsG0580024488.01	0.842895	1.955102e-58	3.006917e-55
MsG0080048978.01	MsG0580024488.01	-0.809143	2.189312e-50	1.275359e-47
MsG0080049015.01	MsG0580024488.01	-0.802349	5.851000e-49	2.859757e-46

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PPI

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Gene1	Gene2	Type
MsG0380011687.01	MsG0580024488.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024488.01.T01	MTR_5g012000	97.826	184	4	0	1	184	34	217	5.81e-134	374
MsG0580024488.01.T01	MTR_4g101760	64.641	181	58	1	5	179	23	203	1.48e-82	243
MsG0580024488.01.T01	MTR_3g008840	53.571	168	77	1	11	178	21	187	8.53e-65	197
MsG0580024488.01.T01	MTR_3g008820	51.190	168	81	1	11	178	20	186	3.36e-62	191
MsG0580024488.01.T01	MTR_4g035905	43.030	165	91	1	15	179	36	197	6.09e-45	147
MsG0580024488.01.T01	MTR_4g035870	42.073	164	92	1	15	178	36	196	7.20e-42	139
MsG0580024488.01.T01	MTR_4g035880	42.073	164	92	1	15	178	36	196	2.06e-41	138
MsG0580024488.01.T01	MTR_4g025550	39.888	178	95	4	15	183	32	206	9.66e-39	132
MsG0580024488.01.T01	MTR_4g025610	39.773	176	98	4	15	183	32	206	1.90e-37	129
MsG0580024488.01.T01	MTR_4g025590	38.636	176	96	4	15	181	3	175	1.02e-36	126

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024488.01.T01	AT4G12390	56.805	169	69	2	15	180	39	206	1.13e-60	187
MsG0580024488.01.T01	AT1G62770	53.514	185	77	5	8	184	21	204	1.50e-58	182
MsG0580024488.01.T01	AT4G25260	47.977	173	89	1	7	178	29	201	5.34e-55	173
MsG0580024488.01.T01	AT5G62350	47.273	165	86	1	15	178	38	202	7.64e-55	172
MsG0580024488.01.T01	AT3G47380	46.988	166	87	1	14	178	37	202	1.88e-51	164
MsG0580024488.01.T01	AT5G62360	41.818	165	92	2	14	178	42	202	2.38e-45	149
MsG0580024488.01.T01	AT1G62760	43.353	173	88	2	11	179	143	309	5.62e-42	143
MsG0580024488.01.T01	AT1G14890	41.566	166	90	2	14	177	57	217	6.79e-39	132
MsG0580024488.01.T01	AT2G01610	40.113	177	93	3	14	177	43	219	2.09e-37	129
MsG0580024488.01.T01	AT5G20740	40.361	166	90	3	14	174	32	193	1.18e-35	124

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Find 48 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACCAAAGCGAGTGTGTTGC+TGG	0.408904	5:-5507683	None:intergenic
AAGGAGTTTCTGCAGTTCGA+TGG	0.560457	5:-5507723	None:intergenic
AATTGTTAAGGCTGCCATCA+AGG	0.538854	5:+5507633	MsG0580024488.01.T01:CDS
ACAAGACTGCATAGAAAACA+TGG	0.577763	5:+5507441	MsG0580024488.01.T01:CDS
ACAGTCTTAGCCAATCGGTT+AGG	0.388918	5:+5507476	MsG0580024488.01.T01:CDS
ACATGAACAACGTGCAGACT+TGG	0.438369	5:+5507545	MsG0580024488.01.T01:CDS
ACTTGTCTTGATGGCTTTGC+TGG	0.284433	5:+5507595	MsG0580024488.01.T01:CDS
AGAAAACATGGGTGACAGTT+TGG	0.352972	5:+5507453	MsG0580024488.01.T01:CDS
AGACATTGAACGCATAAAAC+AGG	0.460054	5:-5507268	None:intergenic
AGCCAATCGGTTAGGGAGTT+AGG	0.439103	5:+5507484	MsG0580024488.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GTTTGTACAGAAGATATCAA+AGG	+	Chr5:5507384-5507403	MsG0580024488.01.T01:CDS	30.0%
TTAACAATTCCATTCATGGA+AGG	-	Chr5:5507622-5507641	None:intergenic	30.0%
TTCCATGAATGGAATTGTTA+AGG	+	Chr5:5507621-5507640	MsG0580024488.01.T01:CDS	30.0%
ACAAGACTGCATAGAAAACA+TGG	+	Chr5:5507441-5507460	MsG0580024488.01.T01:CDS	35.0%
AGACATTGAACGCATAAAAC+AGG	-	Chr5:5507271-5507290	None:intergenic	35.0%
AGCCTTAACAATTCCATTCA+TGG	-	Chr5:5507626-5507645	None:intergenic	35.0%
CAAGACTGCATAGAAAACAT+GGG	+	Chr5:5507442-5507461	MsG0580024488.01.T01:CDS	35.0%
GATGATAACACTTGTCTTGA+TGG	+	Chr5:5507586-5507605	MsG0580024488.01.T01:CDS	35.0%
TTATGCGTTCAATGTCTCAT+GGG	+	Chr5:5507274-5507293	MsG0580024488.01.T01:CDS	35.0%
TTTATGCGTTCAATGTCTCA+TGG	+	Chr5:5507273-5507292	MsG0580024488.01.T01:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	5507193	5507747	5507193	ID=MsG0580024488.01;Name=MsG0580024488.01
Chr5	mRNA	5507193	5507747	5507193	ID=MsG0580024488.01.T01;Parent=MsG0580024488.01;Name=MsG0580024488.01.T01;_AED=0.44;_eAED=0.44;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|184
Chr5	exon	5507193	5507747	5507193	ID=MsG0580024488.01.T01:exon:19081;Parent=MsG0580024488.01.T01
Chr5	CDS	5507193	5507747	5507193	ID=MsG0580024488.01.T01:cds;Parent=MsG0580024488.01.T01

Gene Sequence

>MsG0580024488.01.T01

ATGAGCCTTGTGACTGCAGAATCCTCTATAACCCCATCAGAGTTCATCAAGTCCTCATGTAGAGCTACTCGCTATCCTGTTTTATGCGTTCAATGTCTCATGGGGTATGCGAGCGTGATTGGCCAAAGCGAGCGACAACTAACGATAACCGCTCTATCAGTGAGCATATCCAGGACACGATCGAGTGCATCGTTTGTACAGAAGATATCAAAGGCAAGAGGCATCAAGCCAAGGGAGTACAGAGCCGTACAAGACTGCATAGAAAACATGGGTGACAGTTTGGACAGTCTTAGCCAATCGGTTAGGGAGTTAGGCAGTATTGGTCATGCTGTTGGAGAGGACTTCGTGTGGCACATGAACAACGTGCAGACTTGGGTCAGTGCTGCACTTACTGATGATAACACTTGTCTTGATGGCTTTGCTGGTCCTTCCATGAATGGAATTGTTAAGGCTGCCATCAAGGACAGGGTTGTCAATGTTGCTCAAGTTACCAGCAACACACTCGCTTTGGTTAATCACTTCGCCTCAAGCCATCGAACTGCAGAAACTCCTTAA

Protein sequence

>MsG0580024488.01.T01

MSLVTAESSITPSEFIKSSCRATRYPVLCVQCLMGYASVIGQSERQLTITALSVSISRTRSSASFVQKISKARGIKPREYRAVQDCIENMGDSLDSLSQSVRELGSIGHAVGEDFVWHMNNVQTWVSAALTDDNTCLDGFAGPSMNGIVKAAIKDRVVNVAQVTSNTLALVNHFASSHRTAETP*