Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012109.01.T01 | XP_003598460.2 | 96.939 | 98 | 3 | 0 | 1 | 98 | 38 | 135 | 6.32E-63 | 197 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012109.01.T01 | I3S4V2 | 96.939 | 98 | 3 | 0 | 1 | 98 | 38 | 135 | 3.02e-63 | 197 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380012109.01 | MsG0780038911.01 | 0.808089 | 3.675413e-50 | 2.082343e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012109.01.T01 | MTR_3g013980 | 96.939 | 98 | 3 | 0 | 1 | 98 | 31 | 128 | 1.24e-66 | 196 |
| MsG0380012109.01.T01 | MTR_8g040080 | 44.554 | 101 | 46 | 3 | 1 | 97 | 23 | 117 | 1.17e-16 | 69.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 12 sgRNAs with CRISPR-Local
Find 11 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATTGAAGTTGTCCATTTAA+TGG | 0.149180 | 3:+10990556 | None:intergenic |
| TCCTCAATGGAGCCATTAAA+TGG | 0.313472 | 3:-10990568 | MsG0380012109.01.T01:CDS |
| GCACTAGTCTTCACTTTCAT+AGG | 0.354513 | 3:+10990805 | None:intergenic |
| TTATGCCTGATTACTCTACT+TGG | 0.443981 | 3:-10990621 | MsG0380012109.01.T01:CDS |
| CAATGTTGCTGATATGTTTG+TGG | 0.462429 | 3:-10990737 | MsG0380012109.01.T01:CDS |
| TTCATCCAAGTAGAGTAATC+AGG | 0.472724 | 3:+10990616 | None:intergenic |
| TTACAATTTGAGATCCTCAA+TGG | 0.478064 | 3:-10990581 | MsG0380012109.01.T01:CDS |
| TCCATTTAATGGCTCCATTG+AGG | 0.500948 | 3:+10990567 | None:intergenic |
| CACAAACATATCAGCAACAT+TGG | 0.506499 | 3:+10990738 | None:intergenic |
| AGTGATAATAATTCTAGTGA+AGG | 0.551796 | 3:-10990646 | MsG0380012109.01.T01:CDS |
| AAACAAATTCAATTCAACAA+TGG | 0.630769 | 3:-10990672 | MsG0380012109.01.T01:CDS |
| GCACTTGTACAAGAGCTCAC+AGG | 0.635402 | 3:-10990769 | MsG0380012109.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAACAAATTCAATTCAACAA+TGG | - | Chr3:10990658-10990677 | MsG0380012109.01.T01:CDS | 20.0% |
| ! | AGTGATAATAATTCTAGTGA+AGG | - | Chr3:10990684-10990703 | MsG0380012109.01.T01:CDS | 25.0% |
| ! | TATTGAAGTTGTCCATTTAA+TGG | + | Chr3:10990777-10990796 | None:intergenic | 25.0% |
| ! | TTACAATTTGAGATCCTCAA+TGG | - | Chr3:10990749-10990768 | MsG0380012109.01.T01:CDS | 30.0% |
| CAATGTTGCTGATATGTTTG+TGG | - | Chr3:10990593-10990612 | MsG0380012109.01.T01:CDS | 35.0% | |
| CACAAACATATCAGCAACAT+TGG | + | Chr3:10990595-10990614 | None:intergenic | 35.0% | |
| TTATGCCTGATTACTCTACT+TGG | - | Chr3:10990709-10990728 | MsG0380012109.01.T01:CDS | 35.0% | |
| TTCATCCAAGTAGAGTAATC+AGG | + | Chr3:10990717-10990736 | None:intergenic | 35.0% | |
| TCCATTTAATGGCTCCATTG+AGG | + | Chr3:10990766-10990785 | None:intergenic | 40.0% | |
| TCCTCAATGGAGCCATTAAA+TGG | - | Chr3:10990762-10990781 | MsG0380012109.01.T01:CDS | 40.0% | |
| GCACTTGTACAAGAGCTCAC+AGG | - | Chr3:10990561-10990580 | MsG0380012109.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 10990528 | 10990824 | 10990528 | ID=MsG0380012109.01;Name=MsG0380012109.01 |
| Chr3 | mRNA | 10990528 | 10990824 | 10990528 | ID=MsG0380012109.01.T01;Parent=MsG0380012109.01;Name=MsG0380012109.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|98 |
| Chr3 | exon | 10990528 | 10990824 | 10990528 | ID=MsG0380012109.01.T01:exon:311;Parent=MsG0380012109.01.T01 |
| Chr3 | CDS | 10990528 | 10990824 | 10990528 | ID=MsG0380012109.01.T01:cds;Parent=MsG0380012109.01.T01 |
| Gene Sequence |
| Protein sequence |
