AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780038911.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780038911.01.T01 MTR_7g065130 92.405 79 6 0 1 79 1 79 6.90e-49 149
MsG0780038911.01.T01 MTR_4g019640 62.963 81 28 2 1 79 1 81 3.03e-32 107
MsG0780038911.01.T01 MTR_3g069540 44.156 77 36 2 8 77 17 93 8.73e-16 65.9
MsG0780038911.01.T01 MTR_4g029470 80.000 35 6 1 37 71 56 89 7.85e-12 56.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 28 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTACCTTGGTTTGAAATTA+AGG 0.129246 7:-55275622 None:intergenic
ATGAGAACATGAGGCTTGTT+TGG 0.314452 7:-55275534 None:intergenic
TTTCTCTAAACTTGATATAT+AGG 0.314882 7:-55275913 None:intergenic
CTTGAACCTCTCTAACTTTG+AGG 0.354710 7:+55275453 MsG0780038911.01.T01:five_prime_UTR
GAGTTGGGAGATATATAAAA+AGG 0.385399 7:-55275413 None:intergenic
TTTGTGTCTGCTAGTGCTAA+TGG 0.389664 7:+55275964 MsG0780038911.01.T01:three_prime_UTR
GAGGTACTTGCACTAGTACT+TGG 0.403825 7:+55275805 MsG0780038911.01.T01:CDS
TTTGGTTTGAAACAAGTCAA+TGG 0.446404 7:+55276031 MsG0780038911.01.T01:three_prime_UTR
TGCAAATGTTGTGATGGAAT+TGG 0.452852 7:+55275783 MsG0780038911.01.T01:CDS
TTGGTTTGAAACAAGTCAAT+GGG 0.459688 7:+55276032 MsG0780038911.01.T01:three_prime_UTR
TTAGAGAGGTTCAAGAGCTA+TGG 0.483018 7:-55275445 None:intergenic
GGATAGGGAACTTGTAGGAT+GGG 0.494469 7:-55275392 None:intergenic
TTGTGTCTGCTAGTGCTAAT+GGG 0.495526 7:+55275965 MsG0780038911.01.T01:three_prime_UTR
GCTATGGAGCACTGATGAGT+TGG 0.497002 7:-55275429 None:intergenic
CTATGGAGCACTGATGAGTT+GGG 0.500776 7:-55275428 None:intergenic
GTCCTTGCTTCTATAAGTGA+TGG 0.503145 7:-55275594 None:intergenic
AGGATAGGGAACTTGTAGGA+TGG 0.530247 7:-55275393 None:intergenic
GTTGTTTGCAAATGTTGTGA+TGG 0.534057 7:+55275777 MsG0780038911.01.T01:CDS
AATAACTAGTGTGATTTCCA+AGG 0.557652 7:-55275858 None:intergenic
CTTTCCTTAATTTCAAACCA+AGG 0.576150 7:+55275618 MsG0780038911.01.T01:CDS
ATAGGGAACTTGTAGGATGG+GGG 0.578714 7:-55275390 None:intergenic
GATAGGGAACTTGTAGGATG+GGG 0.578761 7:-55275391 None:intergenic
AACCATCACTTATAGAAGCA+AGG 0.587548 7:+55275592 MsG0780038911.01.T01:CDS
ATAACTTAAAGTGTTCACCT+TGG 0.595348 7:+55275841 MsG0780038911.01.T01:CDS
ATGAAACCTCAAAGTTAGAG+AGG 0.596758 7:-55275459 None:intergenic
AAATGTTGTGATGGAATTGG+AGG 0.597103 7:+55275786 MsG0780038911.01.T01:CDS
ATTAGTAATATGAGAACATG+AGG 0.602881 7:-55275543 None:intergenic
AACAAGTTGCAATAGACATG+TGG 0.663724 7:-55276081 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATTATAAATTAATTACCT+TGG - Chr7:55275638-55275657 None:intergenic 10.0%
!! AAATAAAAAATTAGATCTAG+TGG - Chr7:55275700-55275719 None:intergenic 15.0%
!!! TATTTTGTTATGTATTTTCA+GGG + Chr7:55275727-55275746 MsG0780038911.01.T01:intron 15.0%
!!! TTATTTTGTTATGTATTTTC+AGG + Chr7:55275726-55275745 MsG0780038911.01.T01:intron 15.0%
!! TTTCTCTAAACTTGATATAT+AGG - Chr7:55275916-55275935 None:intergenic 20.0%
!!! AGTTATTTTTGTGATTTTTC+TGG + Chr7:55275874-55275893 MsG0780038911.01.T01:exon 20.0%
!!! ATGATTTTTCATTGATGATA+AGG + Chr7:55276147-55276166 MsG0780038911.01.T01:three_prime_UTR 20.0%
!!! TGATTTTTCATTGATGATAA+GGG + Chr7:55276148-55276167 MsG0780038911.01.T01:three_prime_UTR 20.0%
! ATTAGTAATATGAGAACATG+AGG - Chr7:55275546-55275565 None:intergenic 25.0%
! GGAGATATATAAAAAGGATA+GGG - Chr7:55275410-55275429 None:intergenic 25.0%
! TGTTTCAAACCAAAAAACTA+TGG - Chr7:55276025-55276044 None:intergenic 25.0%
!! ATTACCTTGGTTTGAAATTA+AGG - Chr7:55275625-55275644 None:intergenic 25.0%
!!! TATATGTGTCCATAGTTTTT+TGG + Chr7:55276013-55276032 MsG0780038911.01.T01:three_prime_UTR 25.0%
AATAACTAGTGTGATTTCCA+AGG - Chr7:55275861-55275880 None:intergenic 30.0%
CTTTCCTTAATTTCAAACCA+AGG + Chr7:55275618-55275637 MsG0780038911.01.T01:CDS 30.0%
GAGTTGGGAGATATATAAAA+AGG - Chr7:55275416-55275435 None:intergenic 30.0%
GGGAGATATATAAAAAGGAT+AGG - Chr7:55275411-55275430 None:intergenic 30.0%
! ATAACTTAAAGTGTTCACCT+TGG + Chr7:55275841-55275860 MsG0780038911.01.T01:CDS 30.0%
! TCAAAGATTTTTGCAACACT+TGG + Chr7:55275753-55275772 MsG0780038911.01.T01:CDS 30.0%
! TTGGTTTGAAACAAGTCAAT+GGG + Chr7:55276032-55276051 MsG0780038911.01.T01:three_prime_UTR 30.0%
!! TTTGGTTTGAAACAAGTCAA+TGG + Chr7:55276031-55276050 MsG0780038911.01.T01:three_prime_UTR 30.0%
AAAAAGGATAGGGAACTTGT+AGG - Chr7:55275400-55275419 None:intergenic 35.0%
AACAAGTTGCAATAGACATG+TGG - Chr7:55276084-55276103 None:intergenic 35.0%
AACCATCACTTATAGAAGCA+AGG + Chr7:55275592-55275611 MsG0780038911.01.T01:CDS 35.0%
AATTAGATCTAGTGGAAGAG+AGG - Chr7:55275692-55275711 None:intergenic 35.0%
ATGAAACCTCAAAGTTAGAG+AGG - Chr7:55275462-55275481 None:intergenic 35.0%
GTTGTTTGCAAATGTTGTGA+TGG + Chr7:55275777-55275796 MsG0780038911.01.T01:CDS 35.0%
! AAATGTTGTGATGGAATTGG+AGG + Chr7:55275786-55275805 MsG0780038911.01.T01:CDS 35.0%
! TGCAAATGTTGTGATGGAAT+TGG + Chr7:55275783-55275802 MsG0780038911.01.T01:CDS 35.0%
CTTGAACCTCTCTAACTTTG+AGG + Chr7:55275453-55275472 MsG0780038911.01.T01:five_prime_UTR 40.0%
GTCCTTGCTTCTATAAGTGA+TGG - Chr7:55275597-55275616 None:intergenic 40.0%
TTAGAGAGGTTCAAGAGCTA+TGG - Chr7:55275448-55275467 None:intergenic 40.0%
TTGTGTCTGCTAGTGCTAAT+GGG + Chr7:55275965-55275984 MsG0780038911.01.T01:three_prime_UTR 40.0%
TTTGTGTCTGCTAGTGCTAA+TGG + Chr7:55275964-55275983 MsG0780038911.01.T01:three_prime_UTR 40.0%
! ATGAGAACATGAGGCTTGTT+TGG - Chr7:55275537-55275556 None:intergenic 40.0%
AGGATAGGGAACTTGTAGGA+TGG - Chr7:55275396-55275415 None:intergenic 45.0%
ATAGGGAACTTGTAGGATGG+GGG - Chr7:55275393-55275412 None:intergenic 45.0%
GATAGGGAACTTGTAGGATG+GGG - Chr7:55275394-55275413 None:intergenic 45.0%
GGATAGGGAACTTGTAGGAT+GGG - Chr7:55275395-55275414 None:intergenic 45.0%
! GAGGTACTTGCACTAGTACT+TGG + Chr7:55275805-55275824 MsG0780038911.01.T01:CDS 45.0%
!! CTATGGAGCACTGATGAGTT+GGG - Chr7:55275431-55275450 None:intergenic 45.0%
!! GCTATGGAGCACTGATGAGT+TGG - Chr7:55275432-55275451 None:intergenic 50.0%
GTAGGATGGGGGATGCTGCT+TGG - Chr7:55275382-55275401 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr7 gene 55275372 55276180 55275372 ID=MsG0780038911.01;Name=MsG0780038911.01
Chr7 mRNA 55275372 55276180 55275372 ID=MsG0780038911.01.T01;Parent=MsG0780038911.01;Name=MsG0780038911.01.T01;_AED=0.41;_eAED=0.41;_QI=156|1|1|1|0|0|2|305|79
Chr7 exon 55275372 55275639 55275372 ID=MsG0780038911.01.T01:exon:7729;Parent=MsG0780038911.01.T01
Chr7 exon 55275748 55276180 55275748 ID=MsG0780038911.01.T01:exon:7730;Parent=MsG0780038911.01.T01
Chr7 five_prime_UTR 55275372 55275527 55275372 ID=MsG0780038911.01.T01:five_prime_utr;Parent=MsG0780038911.01.T01
Chr7 CDS 55275528 55275639 55275528 ID=MsG0780038911.01.T01:cds;Parent=MsG0780038911.01.T01
Chr7 CDS 55275748 55275875 55275748 ID=MsG0780038911.01.T01:cds;Parent=MsG0780038911.01.T01
Chr7 three_prime_UTR 55275876 55276180 55275876 ID=MsG0780038911.01.T01:three_prime_utr;Parent=MsG0780038911.01.T01
Gene Sequence

>MsG0780038911.01.T01

ATGAGTCCAAACAAGCCTCATGTTCTCATATTACTAATCATCATGCTTGTTTCAGTTTCTAATAAACCATCACTTATAGAAGCAAGGACACTTTCCTTAATTTCAAACCAAGGGTACTCAAAGATTTTTGCAACACTTGGAGTTGTTTGCAAATGTTGTGATGGAATTGGAGGTACTTGCACTAGTACTTGGACACAATCTTGTAATAACTTAAAGTGTTCACCTTGGAAATCACACTAG

Protein sequence

>MsG0780038911.01.T01

MSPNKPHVLILLIIMLVSVSNKPSLIEARTLSLISNQGYSKIFATLGVVCKCCDGIGGTCTSTWTQSCNNLKCSPWKSH*