Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038911.01.T01 | ACJ86172.1 | 92.405 | 79 | 6 | 0 | 1 | 79 | 1 | 79 | 5.70E-45 | 149 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048137.01 | MsG0780038911.01 | 0.836229 | 1.057492e-56 | 1.324584e-53 |
| MsG0080048197.01 | MsG0780038911.01 | 0.807150 | 5.816929e-50 | 3.215717e-47 |
| MsG0080048205.01 | MsG0780038911.01 | 0.806220 | 9.145437e-50 | 4.936516e-47 |
| MsG0080048982.01 | MsG0780038911.01 | 0.827658 | 1.385028e-54 | 1.345633e-51 |
| MsG0080049121.01 | MsG0780038911.01 | 0.811656 | 6.277213e-51 | 3.907806e-48 |
| MsG0180000220.01 | MsG0780038911.01 | 0.844758 | 6.199380e-59 | 1.011013e-55 |
| MsG0180000271.01 | MsG0780038911.01 | 0.845602 | 3.666310e-59 | 6.142009e-56 |
| MsG0180000284.01 | MsG0780038911.01 | 0.856538 | 3.006910e-62 | 7.201598e-59 |
| MsG0180000293.01 | MsG0780038911.01 | 0.803400 | 3.549711e-49 | 1.782113e-46 |
| MsG0180000550.01 | MsG0780038911.01 | 0.825476 | 4.591882e-54 | 4.189707e-51 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038911.01.T01 | MTR_7g065130 | 92.405 | 79 | 6 | 0 | 1 | 79 | 1 | 79 | 6.90e-49 | 149 |
| MsG0780038911.01.T01 | MTR_4g019640 | 62.963 | 81 | 28 | 2 | 1 | 79 | 1 | 81 | 3.03e-32 | 107 |
| MsG0780038911.01.T01 | MTR_3g069540 | 44.156 | 77 | 36 | 2 | 8 | 77 | 17 | 93 | 8.73e-16 | 65.9 |
| MsG0780038911.01.T01 | MTR_4g029470 | 80.000 | 35 | 6 | 1 | 37 | 71 | 56 | 89 | 7.85e-12 | 56.2 |
Find 28 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAATGTTGTGATGGAATTGG+AGG | 0.597103 | 7:+55275786 | MsG0780038911.01.T01:CDS |
| AACAAGTTGCAATAGACATG+TGG | 0.663724 | 7:-55276081 | None:intergenic |
| AACCATCACTTATAGAAGCA+AGG | 0.587548 | 7:+55275592 | MsG0780038911.01.T01:CDS |
| AATAACTAGTGTGATTTCCA+AGG | 0.557652 | 7:-55275858 | None:intergenic |
| AGGATAGGGAACTTGTAGGA+TGG | 0.530247 | 7:-55275393 | None:intergenic |
| ATAACTTAAAGTGTTCACCT+TGG | 0.595348 | 7:+55275841 | MsG0780038911.01.T01:CDS |
| ATAGGGAACTTGTAGGATGG+GGG | 0.578714 | 7:-55275390 | None:intergenic |
| ATGAAACCTCAAAGTTAGAG+AGG | 0.596758 | 7:-55275459 | None:intergenic |
| ATGAGAACATGAGGCTTGTT+TGG | 0.314452 | 7:-55275534 | None:intergenic |
| ATTACCTTGGTTTGAAATTA+AGG | 0.129246 | 7:-55275622 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AATAACTAGTGTGATTTCCA+AGG | - | Chr7:55275861-55275880 | None:intergenic | 30.0% | |
| CTTTCCTTAATTTCAAACCA+AGG | + | Chr7:55275618-55275637 | MsG0780038911.01.T01:CDS | 30.0% | |
| GAGTTGGGAGATATATAAAA+AGG | - | Chr7:55275416-55275435 | None:intergenic | 30.0% | |
| GGGAGATATATAAAAAGGAT+AGG | - | Chr7:55275411-55275430 | None:intergenic | 30.0% | |
| AAAAAGGATAGGGAACTTGT+AGG | - | Chr7:55275400-55275419 | None:intergenic | 35.0% | |
| AACAAGTTGCAATAGACATG+TGG | - | Chr7:55276084-55276103 | None:intergenic | 35.0% | |
| AACCATCACTTATAGAAGCA+AGG | + | Chr7:55275592-55275611 | MsG0780038911.01.T01:CDS | 35.0% | |
| AATTAGATCTAGTGGAAGAG+AGG | - | Chr7:55275692-55275711 | None:intergenic | 35.0% | |
| ATGAAACCTCAAAGTTAGAG+AGG | - | Chr7:55275462-55275481 | None:intergenic | 35.0% | |
| GTTGTTTGCAAATGTTGTGA+TGG | + | Chr7:55275777-55275796 | MsG0780038911.01.T01:CDS | 35.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 55275372 | 55276180 | 55275372 | ID=MsG0780038911.01;Name=MsG0780038911.01 |
| Chr7 | mRNA | 55275372 | 55276180 | 55275372 | ID=MsG0780038911.01.T01;Parent=MsG0780038911.01;Name=MsG0780038911.01.T01;_AED=0.41;_eAED=0.41;_QI=156|1|1|1|0|0|2|305|79 |
| Chr7 | exon | 55275372 | 55275639 | 55275372 | ID=MsG0780038911.01.T01:exon:7729;Parent=MsG0780038911.01.T01 |
| Chr7 | exon | 55275748 | 55276180 | 55275748 | ID=MsG0780038911.01.T01:exon:7730;Parent=MsG0780038911.01.T01 |
| Chr7 | five_prime_UTR | 55275372 | 55275527 | 55275372 | ID=MsG0780038911.01.T01:five_prime_utr;Parent=MsG0780038911.01.T01 |
| Chr7 | CDS | 55275528 | 55275639 | 55275528 | ID=MsG0780038911.01.T01:cds;Parent=MsG0780038911.01.T01 |
| Chr7 | CDS | 55275748 | 55275875 | 55275748 | ID=MsG0780038911.01.T01:cds;Parent=MsG0780038911.01.T01 |
| Chr7 | three_prime_UTR | 55275876 | 55276180 | 55275876 | ID=MsG0780038911.01.T01:three_prime_utr;Parent=MsG0780038911.01.T01 |
| Gene Sequence |
>MsG0780038911.01.T01 ATGAGTCCAAACAAGCCTCATGTTCTCATATTACTAATCATCATGCTTGTTTCAGTTTCTAATAAACCATCACTTATAGAAGCAAGGACACTTTCCTTAATTTCAAACCAAGGGTACTCAAAGATTTTTGCAACACTTGGAGTTGTTTGCAAATGTTGTGATGGAATTGGAGGTACTTGCACTAGTACTTGGACACAATCTTGTAATAACTTAAAGTGTTCACCTTGGAAATCACACTAG |
| Protein sequence |
>MsG0780038911.01.T01 MSPNKPHVLILLIIMLVSVSNKPSLIEARTLSLISNQGYSKIFATLGVVCKCCDGIGGTCTSTWTQSCNNLKCSPWKSH* |
