Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012315.01.T01 | XP_039688243.1 | 99.074 | 108 | 1 | 0 | 1 | 108 | 82 | 189 | 6.13E-72 | 222 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012315.01.T01 | F4J4N3 | 51.402 | 107 | 52 | 0 | 1 | 107 | 165 | 271 | 2.21E-32 | 117 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012315.01.T01 | A0A396INL4 | 99.074 | 108 | 1 | 0 | 1 | 108 | 82 | 189 | 2.93e-72 | 222 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380012315.01 | MsG0380015988.01 | 0.807600 | 4.669847e-50 | 2.611844e-47 |
| MsG0380012315.01 | MsG0880042645.01 | 0.844617 | 6.768369e-59 | 1.098829e-55 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012315.01.T01 | MTR_4g078885 | 63.551 | 107 | 39 | 0 | 1 | 107 | 78 | 184 | 4.61e-47 | 149 |
| MsG0380012315.01.T01 | MTR_3g034030 | 63.551 | 107 | 39 | 0 | 1 | 107 | 78 | 184 | 3.33e-46 | 147 |
| MsG0380012315.01.T01 | MTR_1g056370 | 54.630 | 108 | 48 | 1 | 1 | 107 | 78 | 185 | 4.19e-35 | 119 |
| MsG0380012315.01.T01 | MTR_4g013330 | 51.402 | 107 | 52 | 0 | 1 | 107 | 92 | 198 | 4.04e-33 | 114 |
| MsG0380012315.01.T01 | MTR_4g013350 | 52.336 | 107 | 51 | 0 | 1 | 107 | 90 | 196 | 5.79e-33 | 114 |
| MsG0380012315.01.T01 | MTR_4g013345 | 51.402 | 107 | 52 | 0 | 1 | 107 | 89 | 195 | 1.25e-32 | 113 |
| MsG0380012315.01.T01 | MTR_7g070390 | 47.664 | 107 | 56 | 0 | 1 | 107 | 85 | 191 | 1.61e-32 | 112 |
| MsG0380012315.01.T01 | MTR_1g054525 | 52.336 | 107 | 49 | 2 | 1 | 107 | 87 | 191 | 2.26e-31 | 109 |
| MsG0380012315.01.T01 | MTR_4g013325 | 50.467 | 107 | 53 | 0 | 1 | 107 | 92 | 198 | 5.41e-31 | 108 |
| MsG0380012315.01.T01 | MTR_4g013335 | 51.402 | 107 | 52 | 0 | 1 | 107 | 89 | 195 | 1.15e-30 | 108 |
| MsG0380012315.01.T01 | MTR_4g013320 | 48.598 | 107 | 55 | 0 | 1 | 107 | 92 | 198 | 8.73e-30 | 105 |
| MsG0380012315.01.T01 | MTR_4g073950 | 44.860 | 107 | 59 | 0 | 1 | 107 | 85 | 191 | 1.34e-29 | 105 |
| MsG0380012315.01.T01 | MTR_7g093790 | 51.852 | 108 | 49 | 3 | 2 | 107 | 84 | 190 | 1.53e-28 | 102 |
| MsG0380012315.01.T01 | MTR_7g093820 | 52.336 | 107 | 50 | 1 | 2 | 107 | 87 | 193 | 1.70e-28 | 102 |
| MsG0380012315.01.T01 | MTR_4g013385 | 42.991 | 107 | 61 | 0 | 1 | 107 | 83 | 189 | 7.33e-28 | 100 |
| MsG0380012315.01.T01 | MTR_0433s0040 | 42.991 | 107 | 61 | 0 | 1 | 107 | 83 | 189 | 7.33e-28 | 100 |
| MsG0380012315.01.T01 | MTR_4g013770 | 46.729 | 107 | 57 | 0 | 1 | 107 | 84 | 190 | 9.13e-28 | 100 |
| MsG0380012315.01.T01 | MTR_7g093850 | 51.402 | 107 | 51 | 1 | 2 | 107 | 87 | 193 | 2.53e-27 | 99.4 |
| MsG0380012315.01.T01 | MTR_4g013310 | 47.619 | 105 | 53 | 1 | 1 | 105 | 92 | 194 | 2.57e-26 | 97.1 |
| MsG0380012315.01.T01 | MTR_7g093830 | 48.598 | 107 | 54 | 1 | 2 | 107 | 86 | 192 | 5.48e-26 | 95.9 |
| MsG0380012315.01.T01 | MTR_5g096120 | 43.925 | 107 | 59 | 1 | 2 | 107 | 82 | 188 | 3.89e-22 | 85.9 |
| MsG0380012315.01.T01 | MTR_7g093870 | 43.363 | 113 | 55 | 3 | 1 | 107 | 83 | 192 | 2.52e-20 | 82.0 |
| MsG0380012315.01.T01 | MTR_3g105640 | 39.252 | 107 | 64 | 1 | 2 | 107 | 81 | 187 | 6.42e-20 | 80.1 |
| MsG0380012315.01.T01 | MTR_4g013355 | 39.252 | 107 | 55 | 1 | 1 | 107 | 82 | 178 | 1.12e-19 | 79.3 |
| MsG0380012315.01.T01 | MTR_3g105630 | 37.383 | 107 | 66 | 1 | 2 | 107 | 82 | 188 | 5.65e-18 | 75.1 |
| MsG0380012315.01.T01 | MTR_4g049550 | 35.789 | 95 | 61 | 0 | 14 | 108 | 84 | 178 | 1.14e-15 | 68.9 |
| MsG0380012315.01.T01 | MTR_4g049570 | 34.737 | 95 | 62 | 0 | 14 | 108 | 84 | 178 | 4.50e-15 | 67.4 |
| MsG0380012315.01.T01 | MTR_4g122110 | 33.645 | 107 | 71 | 0 | 1 | 107 | 73 | 179 | 5.08e-15 | 67.4 |
| MsG0380012315.01.T01 | MTR_4g122130 | 36.782 | 87 | 55 | 0 | 1 | 87 | 73 | 159 | 1.79e-13 | 63.2 |
| MsG0380012315.01.T01 | MTR_1g046490 | 36.364 | 88 | 55 | 1 | 1 | 88 | 127 | 213 | 2.79e-11 | 58.2 |
| MsG0380012315.01.T01 | MTR_1g046800 | 37.500 | 88 | 54 | 1 | 1 | 88 | 141 | 227 | 5.73e-11 | 57.4 |
| MsG0380012315.01.T01 | MTR_1g046500 | 37.500 | 88 | 54 | 1 | 1 | 88 | 141 | 227 | 6.40e-11 | 57.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012315.01.T01 | AT3G58090 | 51.402 | 107 | 52 | 0 | 1 | 107 | 165 | 271 | 2.26e-33 | 117 |
| MsG0380012315.01.T01 | AT1G65870 | 53.271 | 107 | 50 | 0 | 1 | 107 | 83 | 189 | 8.47e-33 | 113 |
| MsG0380012315.01.T01 | AT5G42500 | 50.467 | 107 | 51 | 1 | 1 | 107 | 81 | 185 | 2.80e-31 | 109 |
| MsG0380012315.01.T01 | AT2G21100 | 43.925 | 107 | 60 | 0 | 1 | 107 | 81 | 187 | 2.06e-29 | 104 |
| MsG0380012315.01.T01 | AT2G21100 | 43.925 | 107 | 60 | 0 | 1 | 107 | 81 | 187 | 2.06e-29 | 104 |
| MsG0380012315.01.T01 | AT1G22900 | 48.598 | 107 | 53 | 1 | 1 | 107 | 89 | 193 | 1.01e-28 | 103 |
| MsG0380012315.01.T01 | AT5G42510 | 47.664 | 107 | 54 | 1 | 1 | 107 | 78 | 182 | 5.51e-28 | 100 |
| MsG0380012315.01.T01 | AT3G13650 | 42.991 | 107 | 61 | 0 | 1 | 107 | 79 | 185 | 7.24e-28 | 100 |
| MsG0380012315.01.T01 | AT1G58170 | 44.860 | 107 | 59 | 0 | 1 | 107 | 78 | 184 | 4.64e-27 | 98.6 |
| MsG0380012315.01.T01 | AT1G55210 | 43.925 | 107 | 60 | 0 | 1 | 107 | 80 | 186 | 1.35e-26 | 97.4 |
| MsG0380012315.01.T01 | AT1G55210 | 43.925 | 107 | 60 | 0 | 1 | 107 | 80 | 186 | 1.35e-26 | 97.4 |
| MsG0380012315.01.T01 | AT5G49040 | 45.370 | 108 | 57 | 2 | 1 | 107 | 84 | 190 | 2.19e-26 | 97.1 |
| MsG0380012315.01.T01 | AT3G13660 | 42.056 | 107 | 62 | 0 | 1 | 107 | 18 | 124 | 4.84e-26 | 94.4 |
| MsG0380012315.01.T01 | AT3G13662 | 49.425 | 87 | 44 | 0 | 1 | 87 | 78 | 164 | 1.31e-24 | 92.0 |
| MsG0380012315.01.T01 | AT5G49030 | 50.575 | 87 | 43 | 0 | 1 | 87 | 84 | 170 | 9.50e-24 | 94.7 |
| MsG0380012315.01.T01 | AT4G38700 | 42.342 | 111 | 60 | 2 | 1 | 107 | 79 | 189 | 1.65e-19 | 79.3 |
| MsG0380012315.01.T01 | AT2G21110 | 34.906 | 106 | 69 | 0 | 2 | 107 | 80 | 185 | 1.20e-17 | 74.3 |
| MsG0380012315.01.T01 | AT4G11210 | 34.286 | 105 | 67 | 2 | 1 | 105 | 77 | 179 | 1.51e-12 | 60.8 |
Find 25 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGCCACTGCTAAGAGTCTT+TGG | 0.288310 | 3:+14765735 | MsG0380012315.01.T01:CDS |
| GGTAGAGCTCAAGGGATGTT+TGG | 0.378489 | 3:+14765542 | MsG0380012315.01.T01:CDS |
| AGCTCAAGGGATGTTTGGTT+TGG | 0.396536 | 3:+14765547 | MsG0380012315.01.T01:CDS |
| CCGGAGTGTTTAGGTTTGCT+AGG | 0.397448 | 3:+14765708 | MsG0380012315.01.T01:CDS |
| GCTCTTGTTCTTCAGTTAAG+AGG | 0.398079 | 3:-14765502 | None:intergenic |
| TCTCTAACCGTGTCTTGAAC+AGG | 0.414606 | 3:-14765665 | None:intergenic |
| ACTCCGGTGCCACCGACAAT+AGG | 0.422490 | 3:-14765692 | None:intergenic |
| CTGCCTATTGTCGGTGGCAC+CGG | 0.426676 | 3:+14765689 | MsG0380012315.01.T01:CDS |
| TGCCACTGCTAAGAGTCTTT+GGG | 0.436210 | 3:+14765736 | MsG0380012315.01.T01:CDS |
| CGGAGTGTTTAGGTTTGCTA+GGG | 0.447475 | 3:+14765709 | MsG0380012315.01.T01:CDS |
| ATCCCTTGAGCTCTACCAAC+AGG | 0.451335 | 3:-14765536 | None:intergenic |
| ATTAATCTTGCTTTCACTGA+AGG | 0.457487 | 3:+14765602 | MsG0380012315.01.T01:CDS |
| GGTAGTACTCTTAGTATGCT+TGG | 0.469670 | 3:+14765635 | MsG0380012315.01.T01:CDS |
| TCGGTGGCACCGGAGTGTTT+AGG | 0.470502 | 3:+14765699 | MsG0380012315.01.T01:CDS |
| AAACCTGTTGGTAGAGCTCA+AGG | 0.518436 | 3:+14765533 | MsG0380012315.01.T01:CDS |
| GTTAGAGAACTGCCTATTGT+CGG | 0.550922 | 3:+14765680 | MsG0380012315.01.T01:CDS |
| GAGCTAACATCAAAACCTGT+TGG | 0.568987 | 3:+14765521 | MsG0380012315.01.T01:CDS |
| TTGGCATCTTTGCATGATCG+TGG | 0.594187 | 3:+14765566 | MsG0380012315.01.T01:CDS |
| ATCTTTGCATGATCGTGGAA+TGG | 0.599273 | 3:+14765571 | MsG0380012315.01.T01:CDS |
| AAGAAATCCTGTTCAAGACA+CGG | 0.645101 | 3:+14765658 | MsG0380012315.01.T01:CDS |
| CTCCCAAAGACTCTTAGCAG+TGG | 0.646686 | 3:-14765738 | None:intergenic |
| GGAGTGTTTAGGTTTGCTAG+GGG | 0.669959 | 3:+14765710 | MsG0380012315.01.T01:CDS |
| CCTAGCAAACCTAAACACTC+CGG | 0.676870 | 3:-14765708 | None:intergenic |
| AGAGAACTGCCTATTGTCGG+TGG | 0.682038 | 3:+14765683 | MsG0380012315.01.T01:CDS |
| AACCTGTTGGTAGAGCTCAA+GGG | 0.691584 | 3:+14765534 | MsG0380012315.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATTAATCTTGCTTTCACTGA+AGG | + | Chr3:14765602-14765621 | MsG0380012315.01.T01:CDS | 30.0% | |
| AAGAAATCCTGTTCAAGACA+CGG | + | Chr3:14765658-14765677 | MsG0380012315.01.T01:CDS | 35.0% | |
| ATCTTTGCATGATCGTGGAA+TGG | + | Chr3:14765571-14765590 | MsG0380012315.01.T01:CDS | 40.0% | |
| GAGCTAACATCAAAACCTGT+TGG | + | Chr3:14765521-14765540 | MsG0380012315.01.T01:CDS | 40.0% | |
| GCTCTTGTTCTTCAGTTAAG+AGG | - | Chr3:14765505-14765524 | None:intergenic | 40.0% | |
| GGTAGTACTCTTAGTATGCT+TGG | + | Chr3:14765635-14765654 | MsG0380012315.01.T01:CDS | 40.0% | |
| GTTAGAGAACTGCCTATTGT+CGG | + | Chr3:14765680-14765699 | MsG0380012315.01.T01:CDS | 40.0% | |
| ! | TTCACTGAAGGTGATTTTGC+TGG | + | Chr3:14765614-14765633 | MsG0380012315.01.T01:CDS | 40.0% |
| AGCTCAAGGGATGTTTGGTT+TGG | + | Chr3:14765547-14765566 | MsG0380012315.01.T01:CDS | 45.0% | |
| ATGCCACTGCTAAGAGTCTT+TGG | + | Chr3:14765735-14765754 | MsG0380012315.01.T01:CDS | 45.0% | |
| CCTAGCAAACCTAAACACTC+CGG | - | Chr3:14765711-14765730 | None:intergenic | 45.0% | |
| TCTCTAACCGTGTCTTGAAC+AGG | - | Chr3:14765668-14765687 | None:intergenic | 45.0% | |
| TGCCACTGCTAAGAGTCTTT+GGG | + | Chr3:14765736-14765755 | MsG0380012315.01.T01:CDS | 45.0% | |
| TTGGCATCTTTGCATGATCG+TGG | + | Chr3:14765566-14765585 | MsG0380012315.01.T01:CDS | 45.0% | |
| ! | AAACCTGTTGGTAGAGCTCA+AGG | + | Chr3:14765533-14765552 | MsG0380012315.01.T01:CDS | 45.0% |
| ! | AACCTGTTGGTAGAGCTCAA+GGG | + | Chr3:14765534-14765553 | MsG0380012315.01.T01:CDS | 45.0% |
| !! | CGGAGTGTTTAGGTTTGCTA+GGG | + | Chr3:14765709-14765728 | MsG0380012315.01.T01:CDS | 45.0% |
| !! | GGAGTGTTTAGGTTTGCTAG+GGG | + | Chr3:14765710-14765729 | MsG0380012315.01.T01:CDS | 45.0% |
| AGAGAACTGCCTATTGTCGG+TGG | + | Chr3:14765683-14765702 | MsG0380012315.01.T01:CDS | 50.0% | |
| ATCCCTTGAGCTCTACCAAC+AGG | - | Chr3:14765539-14765558 | None:intergenic | 50.0% | |
| CTCCCAAAGACTCTTAGCAG+TGG | - | Chr3:14765741-14765760 | None:intergenic | 50.0% | |
| GGTAGAGCTCAAGGGATGTT+TGG | + | Chr3:14765542-14765561 | MsG0380012315.01.T01:CDS | 50.0% | |
| ! | CCGGAGTGTTTAGGTTTGCT+AGG | + | Chr3:14765708-14765727 | MsG0380012315.01.T01:CDS | 50.0% |
| CTGCCTATTGTCGGTGGCAC+CGG | + | Chr3:14765689-14765708 | MsG0380012315.01.T01:CDS | 60.0% | |
| ! | TCGGTGGCACCGGAGTGTTT+AGG | + | Chr3:14765699-14765718 | MsG0380012315.01.T01:CDS | 60.0% |
| !! | ACTCCGGTGCCACCGACAAT+AGG | - | Chr3:14765695-14765714 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 14765491 | 14765817 | 14765491 | ID=MsG0380012315.01;Name=MsG0380012315.01 |
| Chr3 | mRNA | 14765491 | 14765817 | 14765491 | ID=MsG0380012315.01.T01;Parent=MsG0380012315.01;Name=MsG0380012315.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|108 |
| Chr3 | exon | 14765491 | 14765817 | 14765491 | ID=MsG0380012315.01.T01:exon:21494;Parent=MsG0380012315.01.T01 |
| Chr3 | CDS | 14765491 | 14765817 | 14765491 | ID=MsG0380012315.01.T01:cds;Parent=MsG0380012315.01.T01 |
| Gene Sequence |
| Protein sequence |
