Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012332.01.T01 | GAU20843.1 | 68.252 | 778 | 221 | 7 | 1 | 772 | 1 | 758 | 0 | 1012 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012332.01.T01 | A0A2Z6MDA5 | 68.252 | 778 | 221 | 7 | 1 | 772 | 1 | 758 | 0.0 | 1012 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280010638.01 | MsG0380012332.01 | 0.803714 | 3.055059e-49 | 1.546137e-46 |
| MsG0280011270.01 | MsG0380012332.01 | 0.800720 | 1.262527e-48 | 5.920721e-46 |
| MsG0380012332.01 | MsG0380012336.01 | 0.839354 | 1.666498e-57 | 2.295581e-54 |
| MsG0380012332.01 | MsG0380012343.01 | 0.807838 | 4.156745e-50 | 2.339453e-47 |
| MsG0380012332.01 | MsG0480021565.01 | 0.823376 | 1.432342e-53 | 1.231011e-50 |
| MsG0380012332.01 | MsG0580029394.01 | 0.824599 | 7.398892e-54 | 6.584860e-51 |
| MsG0380012332.01 | MsG0680030680.01 | 0.801545 | 8.560075e-49 | 4.099008e-46 |
| MsG0380012332.01 | MsG0680031997.01 | 0.807156 | 5.801195e-50 | 3.207514e-47 |
| MsG0380012332.01 | MsG0680032000.01 | 0.816752 | 4.703075e-52 | 3.361301e-49 |
| MsG0380012332.01 | MsG0680032003.01 | 0.819856 | 9.321883e-53 | 7.257826e-50 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012332.01.T01 | MTR_3g023030 | 67.985 | 784 | 221 | 7 | 1 | 772 | 1 | 766 | 0.0 | 1005 |
| MsG0380012332.01.T01 | MTR_3g022600 | 64.631 | 786 | 253 | 9 | 1 | 770 | 1 | 777 | 0.0 | 955 |
| MsG0380012332.01.T01 | MTR_3g027200 | 64.103 | 780 | 263 | 7 | 1 | 772 | 1 | 771 | 0.0 | 955 |
| MsG0380012332.01.T01 | MTR_0425s0020 | 64.122 | 786 | 250 | 9 | 1 | 770 | 1 | 770 | 0.0 | 947 |
| MsG0380012332.01.T01 | MTR_3g023030 | 64.796 | 784 | 212 | 7 | 1 | 772 | 1 | 732 | 0.0 | 942 |
| MsG0380012332.01.T01 | MTR_3g022300 | 63.486 | 786 | 252 | 9 | 1 | 770 | 1 | 767 | 0.0 | 932 |
| MsG0380012332.01.T01 | MTR_3g022130 | 63.063 | 777 | 241 | 7 | 1 | 770 | 1 | 738 | 0.0 | 922 |
| MsG0380012332.01.T01 | MTR_3g027470 | 67.103 | 687 | 202 | 6 | 93 | 772 | 2 | 671 | 0.0 | 900 |
| MsG0380012332.01.T01 | MTR_3g022230 | 62.145 | 774 | 272 | 7 | 1 | 770 | 1 | 757 | 0.0 | 895 |
| MsG0380012332.01.T01 | MTR_3g015260 | 60.334 | 779 | 285 | 8 | 1 | 772 | 3 | 764 | 0.0 | 887 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012332.01.T01 | AT3G14470 | 37.970 | 798 | 436 | 15 | 5 | 772 | 4 | 772 | 1.47e-166 | 509 |
| MsG0380012332.01.T01 | AT3G14470 | 37.970 | 798 | 436 | 15 | 5 | 772 | 4 | 772 | 1.47e-166 | 509 |
| MsG0380012332.01.T01 | AT3G14460 | 33.376 | 788 | 483 | 13 | 5 | 772 | 1 | 766 | 2.78e-125 | 408 |
| MsG0380012332.01.T01 | AT3G50950 | 27.771 | 803 | 488 | 26 | 1 | 770 | 1 | 744 | 4.92e-63 | 227 |
| MsG0380012332.01.T01 | AT3G50950 | 27.771 | 803 | 488 | 26 | 1 | 770 | 1 | 744 | 4.92e-63 | 227 |
| MsG0380012332.01.T01 | AT1G53350 | 28.121 | 761 | 430 | 23 | 1 | 708 | 1 | 697 | 1.43e-57 | 212 |
| MsG0380012332.01.T01 | AT1G58410 | 27.550 | 755 | 468 | 29 | 28 | 746 | 20 | 731 | 4.56e-55 | 204 |
| MsG0380012332.01.T01 | AT1G58410 | 27.550 | 755 | 468 | 29 | 28 | 746 | 20 | 731 | 4.56e-55 | 204 |
| MsG0380012332.01.T01 | AT1G58410 | 27.550 | 755 | 468 | 29 | 28 | 746 | 20 | 731 | 4.56e-55 | 204 |
| MsG0380012332.01.T01 | AT1G58807 | 26.974 | 760 | 479 | 24 | 35 | 759 | 27 | 745 | 1.14e-54 | 203 |
Find 136 sgRNAs with CRISPR-Local
Find 158 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATTCTTATTAAAGGGTT+GGG | 0.377230 | 3:-15044454 | MsG0380012332.01.T01:CDS |
| AAACAATGCTTCCGATTGGA+AGG | 0.501513 | 3:+15045327 | None:intergenic |
| AAAGGTTATAAGTTTGACAA+AGG | 0.589703 | 3:-15045282 | MsG0380012332.01.T01:CDS |
| AAATAGTGAACAAGTGTGGA+GGG | 0.604683 | 3:-15045500 | MsG0380012332.01.T01:CDS |
| AAATCTTGAATCAATTGGCA+GGG | 0.599637 | 3:-15045523 | MsG0380012332.01.T01:CDS |
| AAATTTGCTAAGAATTGATT+TGG | 0.377780 | 3:+15045854 | None:intergenic |
| AACAATGCTTCCGATTGGAA+GGG | 0.631321 | 3:+15045328 | None:intergenic |
| AAGAAGATGCCAAAGCATAT+AGG | 0.456571 | 3:-15044574 | MsG0380012332.01.T01:CDS |
| AAGGTCACAAACTACATCTT+TGG | 0.388784 | 3:-15046108 | MsG0380012332.01.T01:CDS |
| AAGGTGGATTAATCAAGTAT+TGG | 0.531020 | 3:-15045263 | MsG0380012332.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAATCTTGAATCAATTGGCA+GGG | - | Chr3:15045346-15045365 | MsG0380012332.01.T01:CDS | 30.0% | |
| ATATGGTAGAGATGGTTATA+AGG | - | Chr3:15044800-15044819 | MsG0380012332.01.T01:CDS | 30.0% | |
| CATCCAAATCTTGAATCAAT+TGG | - | Chr3:15045341-15045360 | MsG0380012332.01.T01:CDS | 30.0% | |
| GATATGCTGGAATTGAATAA+AGG | - | Chr3:15044693-15044712 | MsG0380012332.01.T01:CDS | 30.0% | |
| GCCTTTAGCTATAAAAACAT+TGG | - | Chr3:15045394-15045413 | MsG0380012332.01.T01:CDS | 30.0% | |
| GTCCAATAACATCAAAATTC+TGG | + | Chr3:15044992-15045011 | None:intergenic | 30.0% | |
| GTGAATGAATCTCTCATATA+TGG | - | Chr3:15044783-15044802 | MsG0380012332.01.T01:CDS | 30.0% | |
| GTTAGATTTCCTATATGCTT+TGG | + | Chr3:15046307-15046326 | None:intergenic | 30.0% | |
| TAGCAATCTCATCTAATAGT+TGG | + | Chr3:15044530-15044549 | None:intergenic | 30.0% | |
| TATTGCTCTATATTTCCCAA+AGG | - | Chr3:15045569-15045588 | MsG0380012332.01.T01:CDS | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 15044285 | 15046606 | 15044285 | ID=MsG0380012332.01;Name=MsG0380012332.01 |
| Chr3 | mRNA | 15044285 | 15046606 | 15044285 | ID=MsG0380012332.01.T01;Parent=MsG0380012332.01;Name=MsG0380012332.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|773 |
| Chr3 | exon | 15044285 | 15046606 | 15044285 | ID=MsG0380012332.01.T01:exon:742;Parent=MsG0380012332.01.T01 |
| Chr3 | CDS | 15044285 | 15046606 | 15044285 | ID=MsG0380012332.01.T01:cds;Parent=MsG0380012332.01.T01 |
| Gene Sequence |
>MsG0380012332.01.T01 ATGGCTGAGATGATTGTTGAGGCATTTCTTGGATCTTTTGCCGACGTGGTTCTTGAGAGGTTGGCTTCAAGTAATTTCATAGATTACTTTCGTAGAGTCAAACTTGAGACATTGGTGGACAAACTTGAGTCTATACTCAATTCTATCAACCAAGTGCTGGATGATGCAGAAAAGAAGCAGTACGTGAATCCAGACGTGAAGACGTGGCTTGGTGATGTTAAGCATTCTATGTATGAGGCTGACCAACTATTAGATGAGATTGCTACTGATGCACCACTCAAGAAGCTGAAGGCCGAATCTCAACCTTCTTCTAGCAATATTTTCAACTTCATTCCAACTTTTACTAATCCATTTGAATCTAGGATCAAAGAATTGATAAAGAGCCTAGATTCTCTTGCTCAGAAGATTGATATGCTGGAATTGAATAAAGGAACTTGTGCTCGTAATGAAGTTGGATTCAATTCGAAACCTTCAGAAAGGTCACAAACTACATCTTTGGTGAATGAATCTCTCATATATGGTAGAGATGGTTATAAGGAGGACATAATCAACTATTTACTTGAAGACAATGACAATGACAACCTGGTATCAGTAACCAGCATAGTTGGTTTGGGTGGGATGGGAAAGACTACTCTTGCTCAACTTGTGTACAATGACCCCAAGATACAAGAGAGGTTTGAACTTAAAGCTTGGGTTTATGTATCCCAGAATTTTGATGTTATTGGACTCACCAAATCAATTCTTAGCAAATTTCATGCTCCAGCAATCAGTGACGACTTGGATCTACTCCAACGAGAACTACAAAAAATATTAACAGGCAAGAAATATCTACTTGTTGTGGATGATGTTTGGCACAAGAAGGAGGAAGATTGGGAGCAGTTACTTCTTCCATTTAATCATGGATCTTCTGGAAGTAAGATTATTGTCACAACACGTGACAAAGAGTTAGTGTATGTCCTGAAATCCACCAAATTAATTGATTTACAACAATTGGACGAAAGCGACTGCTGGAGTTTATTTGTGACACATGCTTTTCATGGCAAGAGTGTGTATGAACATCCAAATCTTGAATCAATTGGCAGGGAAATAGTGAACAAGTGTGGAGGGTTGCCTTTAGCTATAAAAACATTGGGCCAACTCTTGCGAAAAAAATTCTCTCAACATGAATGGATTAAGTTATTGGAGACTGATATGTGGCATTTATCAGACGCGGAAAATAATATTAATCCAGTACTGAGACTGAGTTACCATAATCTCCCTTCCAATCGGAAGCATTGTTTTTCGTATTGCTCTATATTTCCCAAAGGTTATAAGTTTGACAAAGGTGGATTAATCAAGTATTGGATGGCAGAAGGTTTACTCAAATGTTGCAGATCAGAGAAAACTGAAGAAGAGTTGGGTAATGAAATTTTCAATGATCTCGAGTCAATTTCATTTTTCCAACAATCTAATTCAATGTTTGGTTCTTATGAGAACTGCTTTTTAATGCATGATCTTGTCAATGATTTAGCAAAATCAGTGTCAGGTGAATTTTGTATGCAAATAGAGGGAGCCAGGGTGGAAAGTAGCCTTAAAAGGACACGTCACATTTGGTGCTCTCTTCAATTAAATTGTGTTGATGAATCACTAGAGCCAATTTGTCAGCTTAAGGGACTACGTAGTCTGATACTACAAGGTGGCAGGGGTGTGTCAATAAGCAAAACTGTGCAACGTGATCTGTTTTCAGGACTTAAATTTCTGCAGATGTTAAAATTTAGACATTGCGGTCTCTCAGAGCTAGTTGATGAGATAAGAAATTTAAAGCTTTTGCGTTATCTAGACCTTTCTTACACAAAGATTACAAGGTTACCTGATTCTATTTGTATGTTGTATAATTTGCAAACACTCTTATTGCAAGACTGTGGTAATTTGACAGAGCTCCCTTCAAATTTCTCAAAACTCGTCAATTTACGTCATCTTGAACTTCCTTATGAAAGCCTTCCTTACCCAAGTTTTAAATTTCCTAGTATAAAGAAGATGCCAAAGCATATAGGAAATCTAACCAATCTTCAGGCCTTGACTTATTTTAATGTGGAAGAGCAAAATGAGTCTGATCTTAAGGAGTTGGGGAAACTAAACCATCTTCATGGAAAAATTCTTATTAAAGGGTTGGGTAATGTGATTCATCCTGAAGATGCTGCGACAGCCAATTTAAAAGATAAGAAGCATTTAGAAGAATTAGATTTGACGTTTAATGGTTCAAGAATTAGAGTTGAATGCAATGTATCTGTCTTTGAGGCTCTTCAACCAAACAGCAACTTGAAGAAGCTGTTGGTTATTTGA |
| Protein sequence |
>MsG0380012332.01.T01 MAEMIVEAFLGSFADVVLERLASSNFIDYFRRVKLETLVDKLESILNSINQVLDDAEKKQYVNPDVKTWLGDVKHSMYEADQLLDEIATDAPLKKLKAESQPSSSNIFNFIPTFTNPFESRIKELIKSLDSLAQKIDMLELNKGTCARNEVGFNSKPSERSQTTSLVNESLIYGRDGYKEDIINYLLEDNDNDNLVSVTSIVGLGGMGKTTLAQLVYNDPKIQERFELKAWVYVSQNFDVIGLTKSILSKFHAPAISDDLDLLQRELQKILTGKKYLLVVDDVWHKKEEDWEQLLLPFNHGSSGSKIIVTTRDKELVYVLKSTKLIDLQQLDESDCWSLFVTHAFHGKSVYEHPNLESIGREIVNKCGGLPLAIKTLGQLLRKKFSQHEWIKLLETDMWHLSDAENNINPVLRLSYHNLPSNRKHCFSYCSIFPKGYKFDKGGLIKYWMAEGLLKCCRSEKTEEELGNEIFNDLESISFFQQSNSMFGSYENCFLMHDLVNDLAKSVSGEFCMQIEGARVESSLKRTRHIWCSLQLNCVDESLEPICQLKGLRSLILQGGRGVSISKTVQRDLFSGLKFLQMLKFRHCGLSELVDEIRNLKLLRYLDLSYTKITRLPDSICMLYNLQTLLLQDCGNLTELPSNFSKLVNLRHLELPYESLPYPSFKFPSIKKMPKHIGNLTNLQALTYFNVEEQNESDLKELGKLNHLHGKILIKGLGNVIHPEDAATANLKDKKHLEELDLTFNGSRIRVECNVSVFEALQPNSNLKKLLVI* |
