Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380012399.01 | MsG0480018250.01 | 0.805427 | 1.341752e-49 | 7.096631e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012399.01.T02 | MTR_8g098375 | 96.154 | 26 | 1 | 0 | 1 | 26 | 256 | 281 | 6.77e-12 | 58.9 |
| MsG0380012399.01.T02 | MTR_5g010150 | 92.308 | 26 | 2 | 0 | 1 | 26 | 256 | 281 | 1.27e-11 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012399.01.T02 | AT4G00430 | 92.308 | 26 | 2 | 0 | 1 | 26 | 256 | 281 | 1.71e-11 | 57.8 |
| MsG0380012399.01.T02 | AT4G23400 | 92.308 | 26 | 2 | 0 | 1 | 26 | 256 | 281 | 2.75e-11 | 57.4 |
| MsG0380012399.01.T02 | AT1G01620 | 88.462 | 26 | 3 | 0 | 1 | 26 | 255 | 280 | 6.34e-11 | 56.2 |
Find 12 sgRNAs with CRISPR-Local
Find 17 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATCTAGCTCACAGGTTTAA+AGG | 0.197029 | 3:-15975386 | MsG0380012399.01.T02:CDS |
| TTCTGGGTTGGACCATTTAT+TGG | 0.216605 | 3:-15975566 | MsG0380012399.01.T01:CDS |
| GCAAGAGCAGCTCCAATAAA+TGG | 0.282926 | 3:+15975554 | None:intergenic |
| ATCTAGCTCACAGGTTTAAA+GGG | 0.353925 | 3:-15975385 | MsG0380012399.01.T02:CDS |
| AGATGGCTCGGATGACAATC+TGG | 0.450464 | 3:+15975519 | None:intergenic |
| TTGCAGTGGATATTCTGGGT+TGG | 0.527082 | 3:-15975578 | MsG0380012399.01.T01:intron |
| CATTGAATGAAACAACCTCT+TGG | 0.529807 | 3:-15975418 | MsG0380012399.01.T02:CDS |
| CAAACACAACAGCAGCAAGA+TGG | 0.581074 | 3:+15975502 | None:intergenic |
| ACAACAGCAGCAAGATGGCT+CGG | 0.597911 | 3:+15975507 | None:intergenic |
| ATGAGCAAGATCTAGCTCAC+AGG | 0.618206 | 3:-15975394 | MsG0380012399.01.T02:CDS |
| TAGATCTTGCTCATACCAAG+AGG | 0.668311 | 3:+15975403 | None:intergenic |
| TGGCTCGGATGACAATCTGG+TGG | 0.725105 | 3:+15975522 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAAATTTGTTCATAAAAATA+AGG | + | Chr3:15975402-15975421 | None:intergenic | 10.0% |
| !!! | TTATTGATGATATTTTGCAG+TGG | - | Chr3:15975468-15975487 | MsG0380012399.01.T02:CDS | 25.0% |
| ! | ATATTTTGCAGTGGATATTC+TGG | - | Chr3:15975477-15975496 | MsG0380012399.01.T02:CDS | 30.0% |
| ! | TATTTTGCAGTGGATATTCT+GGG | - | Chr3:15975478-15975497 | MsG0380012399.01.T02:CDS | 30.0% |
| AAGGTGATAGAAAACGTACA+TGG | + | Chr3:15975383-15975402 | None:intergenic | 35.0% | |
| CATTGAATGAAACAACCTCT+TGG | - | Chr3:15975642-15975661 | MsG0380012399.01.T01:intron | 35.0% | |
| ! | ATCTAGCTCACAGGTTTAAA+GGG | - | Chr3:15975675-15975694 | MsG0380012399.01.T01:intron | 35.0% |
| TAGATCTTGCTCATACCAAG+AGG | + | Chr3:15975660-15975679 | None:intergenic | 40.0% | |
| ! | GATCTAGCTCACAGGTTTAA+AGG | - | Chr3:15975674-15975693 | MsG0380012399.01.T01:intron | 40.0% |
| ! | TTCTGGGTTGGACCATTTAT+TGG | - | Chr3:15975494-15975513 | MsG0380012399.01.T01:CDS | 40.0% |
| CAAACACAACAGCAGCAAGA+TGG | + | Chr3:15975561-15975580 | None:intergenic | 45.0% | |
| GCAAGAGCAGCTCCAATAAA+TGG | + | Chr3:15975509-15975528 | None:intergenic | 45.0% | |
| TTGCAGTGGATATTCTGGGT+TGG | - | Chr3:15975482-15975501 | MsG0380012399.01.T02:CDS | 45.0% | |
| ! | ATGAGCAAGATCTAGCTCAC+AGG | - | Chr3:15975666-15975685 | MsG0380012399.01.T01:intron | 45.0% |
| ACAACAGCAGCAAGATGGCT+CGG | + | Chr3:15975556-15975575 | None:intergenic | 50.0% | |
| ! | AGATGGCTCGGATGACAATC+TGG | + | Chr3:15975544-15975563 | None:intergenic | 50.0% |
| ! | TGGCTCGGATGACAATCTGG+TGG | + | Chr3:15975541-15975560 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 15975376 | 15975706 | 15975376 | ID=MsG0380012399.01;Name=MsG0380012399.01 |
| Chr3 | mRNA | 15975483 | 15975706 | 15975483 | ID=MsG0380012399.01.T01;Parent=MsG0380012399.01;Name=MsG0380012399.01.T01;_AED=0.50;_eAED=1.00;_QI=0|0|0|1|0|0|2|0|39 |
| Chr3 | exon | 15975699 | 15975706 | 15975699 | ID=MsG0380012399.01.T01:exon:18162;Parent=MsG0380012399.01.T01 |
| Chr3 | exon | 15975483 | 15975594 | 15975483 | ID=MsG0380012399.01.T01:exon:18163;Parent=MsG0380012399.01.T01 |
| Chr3 | CDS | 15975699 | 15975706 | 15975699 | ID=MsG0380012399.01.T01:cds;Parent=MsG0380012399.01.T01 |
| Chr3 | CDS | 15975483 | 15975594 | 15975483 | ID=MsG0380012399.01.T01:cds;Parent=MsG0380012399.01.T01 |
| Chr3 | mRNA | 15975376 | 15975597 | 15975376 | ID=MsG0380012399.01.T02;Parent=MsG0380012399.01;Name=MsG0380012399.01.T02;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|73 |
| Chr3 | exon | 15975376 | 15975597 | 15975376 | ID=MsG0380012399.01.T02:exon:18164;Parent=MsG0380012399.01.T02 |
| Chr3 | CDS | 15975376 | 15975597 | 15975376 | ID=MsG0380012399.01.T02:cds;Parent=MsG0380012399.01.T02 |
| Gene Sequence |
| Protein sequence |
