Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012457.01.T01 | KEH33110.1 | 87.5 | 128 | 16 | 0 | 1 | 128 | 1 | 128 | 8.10E-78 | 236 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012457.01.T01 | A0A072V4J5 | 87.500 | 128 | 16 | 0 | 1 | 128 | 1 | 128 | 3.87e-78 | 236 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0380012457.01 | MsG0180006180.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012457.01.T01 | MTR_3g023770 | 87.500 | 128 | 16 | 0 | 1 | 128 | 1 | 128 | 9.81e-82 | 236 |
| MsG0380012457.01.T01 | MTR_7g090270 | 65.347 | 101 | 34 | 1 | 25 | 125 | 5 | 104 | 1.40e-39 | 136 |
| MsG0380012457.01.T01 | MTR_1g051245 | 55.856 | 111 | 49 | 0 | 17 | 127 | 1 | 111 | 1.06e-38 | 132 |
| MsG0380012457.01.T01 | MTR_7g090290 | 55.208 | 96 | 43 | 0 | 41 | 136 | 1 | 96 | 1.21e-31 | 116 |
| MsG0380012457.01.T01 | MTR_8g027760 | 51.754 | 114 | 54 | 1 | 23 | 135 | 48 | 161 | 1.43e-31 | 114 |
| MsG0380012457.01.T01 | MTR_8g027760 | 51.754 | 114 | 54 | 1 | 23 | 135 | 48 | 161 | 5.53e-31 | 114 |
| MsG0380012457.01.T01 | MTR_4g124580 | 53.846 | 65 | 28 | 1 | 66 | 128 | 2 | 66 | 6.81e-13 | 63.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012457.01.T01 | AT5G04090 | 51.639 | 122 | 58 | 1 | 7 | 127 | 8 | 129 | 2.96e-34 | 121 |
| MsG0380012457.01.T01 | AT3G10250 | 53.636 | 110 | 50 | 1 | 17 | 125 | 1 | 110 | 3.57e-33 | 118 |
| MsG0380012457.01.T01 | AT3G10250 | 53.636 | 110 | 50 | 1 | 17 | 125 | 1 | 110 | 9.76e-33 | 117 |
| MsG0380012457.01.T01 | AT3G10250 | 53.636 | 110 | 50 | 1 | 17 | 125 | 1 | 110 | 9.76e-33 | 117 |
| MsG0380012457.01.T01 | AT3G10250 | 53.636 | 110 | 50 | 1 | 17 | 125 | 1 | 110 | 9.76e-33 | 117 |
| MsG0380012457.01.T01 | AT3G10250 | 53.636 | 110 | 50 | 1 | 17 | 125 | 1 | 110 | 9.76e-33 | 117 |
| MsG0380012457.01.T01 | AT5G04090 | 53.571 | 112 | 51 | 1 | 17 | 127 | 1 | 112 | 2.94e-32 | 116 |
| MsG0380012457.01.T01 | AT2G46420 | 51.402 | 107 | 51 | 1 | 19 | 124 | 41 | 147 | 2.50e-29 | 109 |
| MsG0380012457.01.T01 | AT3G61700 | 50.926 | 108 | 51 | 1 | 19 | 124 | 42 | 149 | 2.96e-29 | 109 |
| MsG0380012457.01.T01 | AT3G61700 | 50.926 | 108 | 51 | 1 | 19 | 124 | 44 | 151 | 3.95e-29 | 108 |
| MsG0380012457.01.T01 | AT3G61700 | 50.926 | 108 | 51 | 1 | 19 | 124 | 44 | 151 | 3.96e-29 | 108 |
| MsG0380012457.01.T01 | AT3G61700 | 50.926 | 108 | 51 | 1 | 19 | 124 | 44 | 151 | 3.96e-29 | 108 |
| MsG0380012457.01.T01 | AT2G46420 | 51.402 | 107 | 51 | 1 | 19 | 124 | 41 | 147 | 4.47e-29 | 108 |
| MsG0380012457.01.T01 | AT2G46420 | 51.402 | 107 | 51 | 1 | 19 | 124 | 41 | 147 | 4.47e-29 | 108 |
| MsG0380012457.01.T01 | AT5G04090 | 55.952 | 84 | 36 | 1 | 45 | 127 | 1 | 84 | 1.08e-22 | 90.5 |
| MsG0380012457.01.T01 | AT5G04090 | 55.952 | 84 | 36 | 1 | 45 | 127 | 1 | 84 | 1.08e-22 | 90.5 |
| MsG0380012457.01.T01 | AT2G29605 | 37.037 | 81 | 51 | 0 | 27 | 107 | 19 | 99 | 1.43e-12 | 63.9 |
| MsG0380012457.01.T01 | AT5G04090 | 54.098 | 61 | 27 | 1 | 68 | 127 | 11 | 71 | 1.12e-11 | 60.8 |
| MsG0380012457.01.T01 | AT5G04090 | 54.098 | 61 | 27 | 1 | 68 | 127 | 11 | 71 | 1.12e-11 | 60.8 |
| MsG0380012457.01.T01 | AT5G04090 | 54.098 | 61 | 27 | 1 | 68 | 127 | 11 | 71 | 1.12e-11 | 60.8 |
| MsG0380012457.01.T01 | AT3G55240 | 40.000 | 80 | 48 | 0 | 30 | 109 | 10 | 89 | 3.08e-11 | 56.6 |
Find 21 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTGTTTCCACTATTTCTTT+TGG | 0.243585 | 3:+16777040 | None:intergenic |
| AAGTTTCTACCTACGCATTT+TGG | 0.257375 | 3:-16776625 | MsG0380012457.01.T01:CDS |
| GAACCCGGGTTTACTAAAAT+AGG | 0.332214 | 3:-16776998 | MsG0380012457.01.T01:intron |
| CCTTCACCACCCAATGCTAA+TGG | 0.357959 | 3:-16776667 | MsG0380012457.01.T01:CDS |
| CCGAGCTTATTATACAAGAT+TGG | 0.370335 | 3:-16776770 | MsG0380012457.01.T01:CDS |
| AGAGAAACAAAAGCAGATAA+TGG | 0.372061 | 3:-16776710 | MsG0380012457.01.T01:CDS |
| GAAAGTTGCAATATGCCAAT+TGG | 0.416390 | 3:-16777857 | None:intergenic |
| TCACAGATTCACTTGGCAAA+TGG | 0.440639 | 3:+16777801 | None:intergenic |
| TATGATCCCAAAAGAAATAG+TGG | 0.444646 | 3:-16777047 | MsG0380012457.01.T01:CDS |
| ACACGCTTCATTGATCCAAT+TGG | 0.464507 | 3:+16777842 | None:intergenic |
| TTACAGGTGGAAAATCTCAT+AGG | 0.488142 | 3:-16777088 | MsG0380012457.01.T01:intron |
| GACATGTGATCCATTAGCAT+TGG | 0.501685 | 3:+16776657 | None:intergenic |
| CCATTAGCATTGGGTGGTGA+AGG | 0.503070 | 3:+16776667 | None:intergenic |
| ACATGTGATCCATTAGCATT+GGG | 0.515311 | 3:+16776658 | None:intergenic |
| CCAATCTTGTATAATAAGCT+CGG | 0.533340 | 3:+16776770 | None:intergenic |
| GCGTAGGTAGAAACTTACTA+GGG | 0.561555 | 3:+16776632 | None:intergenic |
| TGCGTAGGTAGAAACTTACT+AGG | 0.562879 | 3:+16776631 | None:intergenic |
| ACACTCTTCACAGATTCACT+TGG | 0.597329 | 3:+16777794 | None:intergenic |
| AAGCTCAATCCAAAATGCGT+AGG | 0.619410 | 3:+16776616 | None:intergenic |
| TGTGATCCATTAGCATTGGG+TGG | 0.703849 | 3:+16776661 | None:intergenic |
| CGTAGGTAGAAACTTACTAG+GGG | 0.711131 | 3:+16776633 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATTATCTATAACAATTAT+AGG | - | Chr3:16776943-16776962 | MsG0380012457.01.T01:intron | 10.0% |
| !!! | AGATTTTAATATATCATATA+TGG | + | Chr3:16777513-16777532 | None:intergenic | 10.0% |
| !! | ATTATTTACACTTGAATAAA+AGG | + | Chr3:16777052-16777071 | None:intergenic | 15.0% |
| !! | TTAAATTACTTATTTACCTT+TGG | - | Chr3:16777192-16777211 | MsG0380012457.01.T01:intron | 15.0% |
| !!! | ATAGTGTTGAATAATTAATA+TGG | - | Chr3:16777103-16777122 | MsG0380012457.01.T01:intron | 15.0% |
| !!! | TAGTGTTGAATAATTAATAT+GGG | - | Chr3:16777104-16777123 | MsG0380012457.01.T01:intron | 15.0% |
| !!! | TTTTATTCAAGTGTAAATAA+TGG | - | Chr3:16777051-16777070 | MsG0380012457.01.T01:CDS | 15.0% |
| !!! | ATAGATAACTTATTTTGTCA+TGG | - | Chr3:16777333-16777352 | MsG0380012457.01.T01:intron | 20.0% |
| !!! | ATGAGGTTTTTATTTTTAGT+TGG | - | Chr3:16777153-16777172 | MsG0380012457.01.T01:intron | 20.0% |
| !!! | GTTTGAATTTTTATAGAATG+AGG | - | Chr3:16777136-16777155 | MsG0380012457.01.T01:intron | 20.0% |
| !!! | TGAGGTTTTTATTTTTAGTT+GGG | - | Chr3:16777154-16777173 | MsG0380012457.01.T01:intron | 20.0% |
| ! | ACACTTGAATAAAAGGTAAA+TGG | + | Chr3:16777045-16777064 | None:intergenic | 25.0% |
| ! | AGTTGTTCACATTGATAAAA+AGG | - | Chr3:16777018-16777037 | MsG0380012457.01.T01:CDS | 25.0% |
| ! | ATATATTCAATCACATGCAA+GGG | + | Chr3:16776736-16776755 | None:intergenic | 25.0% |
| ! | CATAGTAATTAGTAATCCAA+AGG | + | Chr3:16777211-16777230 | None:intergenic | 25.0% |
| ! | GCAACAATCAAGAAATATAT+AGG | + | Chr3:16776773-16776792 | None:intergenic | 25.0% |
| ! | TTGCAAAATTTAGTTGAACT+TGG | + | Chr3:16777569-16777588 | None:intergenic | 25.0% |
| !!! | ATTACCTATTTTAGTAAACC+CGG | + | Chr3:16777466-16777485 | None:intergenic | 25.0% |
| !!! | TTGATTGTTGCTTTAGAATA+GGG | - | Chr3:16776782-16776801 | MsG0380012457.01.T01:CDS | 25.0% |
| AGAGAAACAAAAGCAGATAA+TGG | - | Chr3:16777747-16777766 | MsG0380012457.01.T01:intron | 30.0% | |
| AGTGAATCAAAAATTGAACC+CGG | - | Chr3:16777444-16777463 | MsG0380012457.01.T01:intron | 30.0% | |
| CATATATTCAATCACATGCA+AGG | + | Chr3:16776737-16776756 | None:intergenic | 30.0% | |
| CCAATCTTGTATAATAAGCT+CGG | + | Chr3:16777690-16777709 | None:intergenic | 30.0% | |
| GGGTTTACTAAAATAGGTAA+TGG | - | Chr3:16777465-16777484 | MsG0380012457.01.T01:intron | 30.0% | |
| TATATTCAATCACATGCAAG+GGG | + | Chr3:16776735-16776754 | None:intergenic | 30.0% | |
| TATGATCCCAAAAGAAATAG+TGG | - | Chr3:16777410-16777429 | MsG0380012457.01.T01:intron | 30.0% | |
| TGGTTTACTTGTCTATTTAC+AGG | - | Chr3:16777353-16777372 | MsG0380012457.01.T01:intron | 30.0% | |
| TTTACTTGTCTATTTACAGG+TGG | - | Chr3:16777356-16777375 | MsG0380012457.01.T01:intron | 30.0% | |
| ! | AATGTAGCAATCACATCTTT+TGG | - | Chr3:16777587-16777606 | MsG0380012457.01.T01:intron | 30.0% |
| !! | AGTGTTTCCACTATTTCTTT+TGG | + | Chr3:16777420-16777439 | None:intergenic | 30.0% |
| !! | CTTGATTGTTGCTTTAGAAT+AGG | - | Chr3:16776781-16776800 | MsG0380012457.01.T01:CDS | 30.0% |
| !! | GTGTTTCCACTATTTCTTTT+GGG | + | Chr3:16777419-16777438 | None:intergenic | 30.0% |
| !! | TGATTGTTGCTTTAGAATAG+GGG | - | Chr3:16776783-16776802 | MsG0380012457.01.T01:CDS | 30.0% |
| !!! | GACTTTTCTTCTTTGTTTTG+TGG | - | Chr3:16777487-16777506 | MsG0380012457.01.T01:intron | 30.0% |
| !!! | TTACCTATTTTAGTAAACCC+GGG | + | Chr3:16777465-16777484 | None:intergenic | 30.0% |
| ACATGTGATCCATTAGCATT+GGG | + | Chr3:16777802-16777821 | None:intergenic | 35.0% | |
| CCGAGCTTATTATACAAGAT+TGG | - | Chr3:16777687-16777706 | MsG0380012457.01.T01:intron | 35.0% | |
| GTGAATCAAAAATTGAACCC+GGG | - | Chr3:16777445-16777464 | MsG0380012457.01.T01:intron | 35.0% | |
| TTACAGGTGGAAAATCTCAT+AGG | - | Chr3:16777369-16777388 | MsG0380012457.01.T01:intron | 35.0% | |
| ! | AAGTTTCTACCTACGCATTT+TGG | - | Chr3:16777832-16777851 | MsG0380012457.01.T01:CDS | 35.0% |
| !! | GATTGTTGCTTTAGAATAGG+GGG | - | Chr3:16776784-16776803 | MsG0380012457.01.T01:CDS | 35.0% |
| AAGCTCAATCCAAAATGCGT+AGG | + | Chr3:16777844-16777863 | None:intergenic | 40.0% | |
| ACACGCTTCATTGATCCAAT+TGG | + | Chr3:16776618-16776637 | None:intergenic | 40.0% | |
| ACACTCTTCACAGATTCACT+TGG | + | Chr3:16776666-16776685 | None:intergenic | 40.0% | |
| CAACACTAGACACTCAACAA+TGG | + | Chr3:16777272-16777291 | None:intergenic | 40.0% | |
| CGTAGGTAGAAACTTACTAG+GGG | + | Chr3:16777827-16777846 | None:intergenic | 40.0% | |
| GAACCCGGGTTTACTAAAAT+AGG | - | Chr3:16777459-16777478 | MsG0380012457.01.T01:intron | 40.0% | |
| GACATGTGATCCATTAGCAT+TGG | + | Chr3:16777803-16777822 | None:intergenic | 40.0% | |
| GCGTAGGTAGAAACTTACTA+GGG | + | Chr3:16777828-16777847 | None:intergenic | 40.0% | |
| TCACAGATTCACTTGGCAAA+TGG | + | Chr3:16776659-16776678 | None:intergenic | 40.0% | |
| TGCGTAGGTAGAAACTTACT+AGG | + | Chr3:16777829-16777848 | None:intergenic | 40.0% | |
| TCCACACTGCAAAGATGAGA+GGG | + | Chr3:16777636-16777655 | None:intergenic | 45.0% | |
| TTCCACACTGCAAAGATGAG+AGG | + | Chr3:16777637-16777656 | None:intergenic | 45.0% | |
| ! | TGTGATCCATTAGCATTGGG+TGG | + | Chr3:16777799-16777818 | None:intergenic | 45.0% |
| CCTTCACCACCCAATGCTAA+TGG | - | Chr3:16777790-16777809 | MsG0380012457.01.T01:CDS | 50.0% | |
| TCCCTCTCATCTTTGCAGTG+TGG | - | Chr3:16777632-16777651 | MsG0380012457.01.T01:intron | 50.0% | |
| ! | CCATTAGCATTGGGTGGTGA+AGG | + | Chr3:16777793-16777812 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 16776612 | 16777867 | 16776612 | ID=MsG0380012457.01;Name=MsG0380012457.01 |
| Chr3 | mRNA | 16776612 | 16777867 | 16776612 | ID=MsG0380012457.01.T01;Parent=MsG0380012457.01;Name=MsG0380012457.01.T01;_AED=0.51;_eAED=0.51;_QI=0|0|0|1|1|1|3|0|139 |
| Chr3 | exon | 16777772 | 16777867 | 16777772 | ID=MsG0380012457.01.T01:exon:21953;Parent=MsG0380012457.01.T01 |
| Chr3 | exon | 16776999 | 16777104 | 16776999 | ID=MsG0380012457.01.T01:exon:21952;Parent=MsG0380012457.01.T01 |
| Chr3 | exon | 16776612 | 16776829 | 16776612 | ID=MsG0380012457.01.T01:exon:21951;Parent=MsG0380012457.01.T01 |
| Chr3 | CDS | 16777772 | 16777867 | 16777772 | ID=MsG0380012457.01.T01:cds;Parent=MsG0380012457.01.T01 |
| Chr3 | CDS | 16776999 | 16777104 | 16776999 | ID=MsG0380012457.01.T01:cds;Parent=MsG0380012457.01.T01 |
| Chr3 | CDS | 16776612 | 16776829 | 16776612 | ID=MsG0380012457.01.T01:cds;Parent=MsG0380012457.01.T01 |
| Gene Sequence |
| Protein sequence |
