AlfalfaGEDB | MsG0180006180.01

M. sativa cultivar ZhongmuNo.1 / MsG0180006180.01

KEGG: -
GO: -
PFAM: PF09713

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180006180.01.T01	XP_039685815.1	100	48	0	0	1	48	1	48	5.22E-26	100

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180006180.01.T01	B7FMI5	100.000	48	0	0	1	48	1	48	2.49e-26	100

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000782.01	MsG0180006180.01	0.815148	1.072286e-51	7.333957e-49
MsG0180006180.01	MsG0280006592.01	0.810481	1.128108e-50	6.807835e-48
MsG0180006180.01	MsG0280008899.01	0.841784	3.851599e-58	5.720353e-55
MsG0180006180.01	MsG0280009421.01	0.803575	3.264778e-49	1.646528e-46
MsG0180006180.01	MsG0380012565.01	0.820611	6.258614e-53	4.975580e-50
MsG0180006180.01	MsG0480023150.01	0.810456	1.142397e-50	6.889277e-48
MsG0180006180.01	MsG0580027955.01	0.816398	5.645197e-52	3.995727e-49
MsG0180006180.01	MsG0680030895.01	0.846385	2.245661e-59	3.858784e-56
MsG0180006180.01	MsG0680031263.01	0.822628	2.139457e-53	1.800660e-50
MsG0180006180.01	MsG0680031708.01	0.802672	5.020048e-49	2.473725e-46
MsG0180006180.01	MsG0780039940.01	0.839606	1.432856e-57	1.988934e-54
MsG0180006180.01	MsG0780040430.01	0.800463	1.424168e-48	6.635376e-46
MsG0180006180.01	MsG0780040431.01	0.802782	4.764692e-49	2.354625e-46

PPI

Gene1	Gene2	Type
MsG0380012457.01	MsG0180006180.01	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180006180.01.T01	MTR_1g115490	100.000	48	0	0	1	48	1	48	6.32e-30	100
MsG0180006180.01.T01	MTR_0001s0190	97.917	48	1	0	1	48	1	48	2.70e-29	99.4
MsG0180006180.01.T01	MTR_7g022840	92.308	39	3	0	10	48	15	53	3.82e-22	81.3
MsG0180006180.01.T01	MTR_7g100335	74.419	43	11	0	6	48	4	46	2.26e-19	73.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180006180.01.T01	AT3G55240	83.333	48	7	1	1	48	1	47	5.05e-24	85.9
MsG0180006180.01.T01	AT5G02580	76.000	50	11	1	1	49	1	50	2.09e-21	78.2
MsG0180006180.01.T01	AT5G02580	75.510	49	11	1	1	48	1	49	1.62e-20	76.6
MsG0180006180.01.T01	AT3G28990	79.545	44	9	0	5	48	4	47	6.73e-20	75.1
MsG0180006180.01.T01	AT5G02580	69.231	39	12	0	10	48	4	42	1.70e-13	58.9
MsG0180006180.01.T01	AT1G10657	52.500	40	19	0	9	48	22	61	3.27e-11	53.5
MsG0180006180.01.T01	AT1G10657	52.500	40	19	0	9	48	22	61	3.80e-11	53.1
MsG0180006180.01.T01	AT1G10657	52.500	40	19	0	9	48	22	61	4.63e-11	53.5
MsG0180006180.01.T01	AT1G10657	52.500	40	19	0	9	48	22	61	5.57e-11	53.5

Find 7 sgRNAs with CRISPR-Local

Find 11 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATACCAGTGGATGTTATAAT+TGG	0.219027	1:+101046677	None:intergenic
TGGATTTATATTTGCATGTT+TGG	0.252690	1:+101046697	None:intergenic
CGCTTCCATGCATTCTTCCT+TGG	0.321815	1:+101046724	None:intergenic
TTCGGCTTCATACATTCACT+TGG	0.510731	1:-101046915	MsG0180006180.01.T01:intron
AATCCAATTATAACATCCAC+TGG	0.539693	1:-101046680	MsG0180006180.01.T01:CDS
CATGACCAAGGAAGAATGCA+TGG	0.570865	1:-101046729	MsG0180006180.01.T01:CDS
TTTGATCTTTCACATGACCA+AGG	0.655387	1:-101046741	MsG0180006180.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CTACAATTGCAAATATAATT+AGG	-	Chr1:101046810-101046829	MsG0180006180.01.T01:intron	20.0%
!	TGGATTTATATTTGCATGTT+TGG	+	Chr1:101046902-101046921	None:intergenic	25.0%
!	TTAAAAATCATACATACCAG+TGG	+	Chr1:101046935-101046954	None:intergenic	25.0%
!!	ATAATTTTTGTCAACATGCA+TGG	+	Chr1:101046784-101046803	None:intergenic	25.0%
	AATCCAATTATAACATCCAC+TGG	-	Chr1:101046916-101046935	MsG0180006180.01.T01:CDS	30.0%
	ATACCAGTGGATGTTATAAT+TGG	+	Chr1:101046922-101046941	None:intergenic	30.0%
	TTTGATCTTTCACATGACCA+AGG	-	Chr1:101046855-101046874	MsG0180006180.01.T01:intron	35.0%
!	AGATCAAACACTTTTCGATC+AGG	+	Chr1:101046843-101046862	None:intergenic	35.0%
	TTCGGCTTCATACATTCACT+TGG	-	Chr1:101046681-101046700	MsG0180006180.01.T01:CDS	40.0%
	CATGACCAAGGAAGAATGCA+TGG	-	Chr1:101046867-101046886	MsG0180006180.01.T01:intron	45.0%
	CGCTTCCATGCATTCTTCCT+TGG	+	Chr1:101046875-101046894	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	101046664	101046954	101046664	ID=MsG0180006180.01;Name=MsG0180006180.01
Chr1	mRNA	101046664	101046954	101046664	ID=MsG0180006180.01.T01;Parent=MsG0180006180.01;Name=MsG0180006180.01.T01;_AED=0.46;_eAED=0.52;_QI=0\|0\|0\|1\|0\|0\|2\|0\|53
Chr1	exon	101046664	101046786	101046664	ID=MsG0180006180.01.T01:exon:12847;Parent=MsG0180006180.01.T01
Chr1	exon	101046916	101046954	101046916	ID=MsG0180006180.01.T01:exon:12846;Parent=MsG0180006180.01.T01
Chr1	CDS	101046916	101046954	101046916	ID=MsG0180006180.01.T01:cds;Parent=MsG0180006180.01.T01
Chr1	CDS	101046664	101046786	101046664	ID=MsG0180006180.01.T01:cds;Parent=MsG0180006180.01.T01

Gene Sequence	>MsG0180006180.01.T01 ATGGGGGACTCGTCTTCTTCGGCTTCATACATTCACTTGGTGCAGCACCTGATCGAAAAGTGTTTGATCTTTCACATGACCAAGGAAGAATGCATGGAAGCGCTCTCCAAACATGCAAATATAAATCCAATTATAACATCCACTGGTATGTATGATTTTTAA
Protein sequence	>MsG0180006180.01.T01 MGDSSSSASYIHLVQHLIEKCLIFHMTKEECMEALSKHANINPIITSTGMYDF*

Copyright © Liu Lab , TPR, XTBG, China.

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