AlfalfaGEDB | MsG0380012525.01

M. sativa cultivar ZhongmuNo.1 / MsG0380012525.01

KEGG: K22279
GO: GO:0004553 GO:0005975
PFAM: PF00232

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380012525.01.T01	XP_003599013.1	77.586	290	25	2	42	300	105	385	4.32E-141	418

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380012525.01.T01	A2SY66	73.494	249	57	1	42	290	104	343	4.45E-129	380

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380012525.01.T01	G7IZC8	77.586	290	25	2	42	300	105	385	2.06e-141	418

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0380012524.01	MsG0380012525.01	0.877523	5.871453e-69	2.961285e-65
MsG0380012525.01	MsG0380015743.01	0.805812	1.114420e-49	5.952840e-47
MsG0380012525.01	MsG0580025859.01	0.801021	1.096138e-48	5.180274e-46

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380012525.01.T01	MTR_3g026620	77.586	290	25	2	42	300	105	385	5.23e-145	418
MsG0380012525.01.T01	MTR_3g026400	73.494	249	57	1	42	290	104	343	3.41e-134	385
MsG0380012525.01.T01	MTR_3g026400	73.494	249	57	1	42	290	73	312	9.71e-132	383
MsG0380012525.01.T01	MTR_3g026400	73.494	249	57	1	42	290	104	343	1.56e-131	383
MsG0380012525.01.T01	MTR_4g066240	56.612	242	97	2	50	290	111	345	7.78e-97	292
MsG0380012525.01.T01	MTR_4g066240	55.200	250	104	2	42	290	103	345	8.48e-96	292
MsG0380012525.01.T01	MTR_4g066240	55.200	250	104	2	42	290	103	345	1.06e-95	292
MsG0380012525.01.T01	MTR_2g098540	57.466	221	93	1	71	290	76	296	4.38e-94	286
MsG0380012525.01.T01	MTR_4g067040	57.207	222	94	1	71	291	123	344	5.51e-94	283
MsG0380012525.01.T01	MTR_4g067040	57.207	222	94	1	71	291	123	344	8.71e-94	283

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380012525.01.T01	AT2G44480	52.439	246	110	1	46	291	4	242	5.49e-92	279
MsG0380012525.01.T01	AT2G44480	52.653	245	109	1	46	290	106	343	9.27e-91	279
MsG0380012525.01.T01	AT2G44480	52.653	245	109	1	46	290	137	374	1.58e-90	280
MsG0380012525.01.T01	AT2G44480	51.779	253	114	2	46	298	137	381	1.15e-89	273
MsG0380012525.01.T01	AT3G18080	50.622	241	110	1	50	290	113	344	1.12e-80	253
MsG0380012525.01.T01	AT3G60130	46.667	270	133	4	25	290	26	288	8.85e-80	249
MsG0380012525.01.T01	AT1G02850	49.333	225	113	1	73	296	112	336	9.08e-80	250
MsG0380012525.01.T01	AT3G60130	46.667	270	133	4	25	290	26	288	1.21e-79	249
MsG0380012525.01.T01	AT2G44450	47.561	246	121	2	46	290	102	340	1.87e-79	250
MsG0380012525.01.T01	AT5G42260	48.571	245	117	3	46	288	102	339	5.20e-79	249

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Find 88 sgRNAs with CRISPR-Local

Find 419 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGCCTCTCCATGATCCAT+TGG	0.487416	3:-19172393	MsG0380012525.01.T01:CDS
AAAGGTGGGTGGCCCGATAA+CGG	0.513836	3:+19172320	None:intergenic
AAAGTGATGAGCAACAATGT+AGG	0.552733	3:+19167427	None:intergenic
AACGAAAACCTGATAGCAGG+TGG	0.655710	3:+19172034	None:intergenic
AACGGAAACCTGATAGCAGG+TGG	0.655710	3:+19172338	None:intergenic
AAGCCTCTCCATGATCCATT+GGG	0.516574	3:-19172392	MsG0380012525.01.T01:CDS
AAGGATAATATTATTGATTG+GGG	0.560825	3:+19166673	None:intergenic
AAGGGAGCAGTCAATCCTAT+GGG	0.598556	3:-19168026	MsG0380012525.01.T01:CDS
AAGTGATGAGCAACAATGTA+GGG	0.499808	3:+19167428	None:intergenic
ACATATTTAGAGCATCTTGC+AGG	0.404850	3:+19167485	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAACAAAGTCTTAGAAATGG+TGG	+	Chr3:19166959-19166978	None:intergenic	30.0%
AACACTTTATAAGTGAGAGA+GGG	+	Chr3:19167306-19167325	None:intergenic	30.0%
AATGCTTTGTGCATAATATG+TGG	+	Chr3:19172194-19172213	None:intergenic	30.0%
ACCTCATTCGTAAGTATTTA+TGG	-	Chr3:19172259-19172278	MsG0380012525.01.T01:intron	30.0%
AGCTCTTGAAGATGAATATA+AGG	-	Chr3:19170973-19170992	MsG0380012525.01.T01:intron	30.0%
AGTAACTGTCGATCTTAATT+CGG	+	Chr3:19171647-19171666	None:intergenic	30.0%
AGTTTCCACGTTAAAAATTC+TGG	+	Chr3:19167661-19167680	None:intergenic	30.0%
ATACACCAAAACTTTGGTAA+TGG	+	Chr3:19170078-19170097	None:intergenic	30.0%
ATTCACACCAAGAAAATCAT+AGG	+	Chr3:19172164-19172183	None:intergenic	30.0%
ATTGTATAAGGCCAAATATC+AGG	-	Chr3:19171380-19171399	MsG0380012525.01.T01:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	19166357	19172423	19166357	ID=MsG0380012525.01;Name=MsG0380012525.01
Chr3	mRNA	19166357	19172423	19166357	ID=MsG0380012525.01.T01;Parent=MsG0380012525.01;Name=MsG0380012525.01.T01;_AED=0.49;_eAED=0.61;_QI=0\|0.42\|0.25\|1\|0.85\|0.75\|8\|0\|300
Chr3	exon	19172289	19172423	19172289	ID=MsG0380012525.01.T01:exon:3989;Parent=MsG0380012525.01.T01
Chr3	exon	19171984	19172062	19171984	ID=MsG0380012525.01.T01:exon:3990;Parent=MsG0380012525.01.T01
Chr3	exon	19167982	19168053	19167982	ID=MsG0380012525.01.T01:exon:3991;Parent=MsG0380012525.01.T01
Chr3	exon	19167763	19167850	19167763	ID=MsG0380012525.01.T01:exon:3992;Parent=MsG0380012525.01.T01
Chr3	exon	19167379	19167631	19167379	ID=MsG0380012525.01.T01:exon:3993;Parent=MsG0380012525.01.T01
Chr3	exon	19166877	19166992	19166877	ID=MsG0380012525.01.T01:exon:3994;Parent=MsG0380012525.01.T01
Chr3	exon	19166605	19166726	19166605	ID=MsG0380012525.01.T01:exon:3995;Parent=MsG0380012525.01.T01
Chr3	exon	19166357	19166394	19166357	ID=MsG0380012525.01.T01:exon:3996;Parent=MsG0380012525.01.T01
Chr3	CDS	19172289	19172423	19172289	ID=MsG0380012525.01.T01:cds;Parent=MsG0380012525.01.T01
Chr3	CDS	19171984	19172062	19171984	ID=MsG0380012525.01.T01:cds;Parent=MsG0380012525.01.T01
Chr3	CDS	19167982	19168053	19167982	ID=MsG0380012525.01.T01:cds;Parent=MsG0380012525.01.T01
Chr3	CDS	19167763	19167850	19167763	ID=MsG0380012525.01.T01:cds;Parent=MsG0380012525.01.T01
Chr3	CDS	19167379	19167631	19167379	ID=MsG0380012525.01.T01:cds;Parent=MsG0380012525.01.T01
Chr3	CDS	19166877	19166992	19166877	ID=MsG0380012525.01.T01:cds;Parent=MsG0380012525.01.T01
Chr3	CDS	19166605	19166726	19166605	ID=MsG0380012525.01.T01:cds;Parent=MsG0380012525.01.T01
Chr3	CDS	19166357	19166394	19166357	ID=MsG0380012525.01.T01:cds;Parent=MsG0380012525.01.T01

Gene Sequence

>MsG0380012525.01.T01

ATGGTATCAAAGCCTCTCCATGATCCATTGGGCCACCTGCTATCAGGTTGGGCATCCATTGGGCCACCTGCTATCAGGTTTCCGTTATCGGGCCACCCACCTTTATGTACACGAACCAAGCCAATAGTGCTGGTCGTTGGGCCACCTGCTATCAGGTTTTCGTTATCGGGCCACCCACCATTTATGTTCACGAATCAAGCCAATAGTGCTGGTCAGGGCAAGGGAGCAGTCAATCCTATGGGAGTTAAATTCTACAATAATGTCATTAATGAGGTCCTAGCAAATGGCTTGATACCCTTTGTAACATTGTTTCATTGGGATCTTCCACGAGCTCTTGAAGATGAATATAAGGGATTTCTAAGTCTTAAAGTAGTGAAGGATTTTGAAGCCTATGCTGATTTTTGCTTCAAGACATTTGGAGATAGGGTTAAGCATTGGGTGACACTTAATGAGCCTGTTTCCTACACCATTAATGGCTACCATGGAGGCACTTCTCCACCTGCAAGATGCTCTAAATATGTTGGAAATTGCTCTACTGGTGACTCAACTACAGAACCCTACATTGTTGCTCATCACTTTATACTTTCTCATGCTGCTGCAGCCAAATTGTATAAGGCCAAATATCAGGCTCATCAAAAAGGAAAAATTGGAATTACCTTGATTACTCACTACTATGAACCATATTCCAACAGTGTTGCCGATCACAAAGCTGCAAGTAGAGCACTTGACTTTTTATTTGGATGGTTTGCTCATCCAATTACATATGGTCACTATCCCCAATCAATAATATTATCCTTGGGTAACAGACTCCCAAAATTCACAAAAGAAGAATCTAAAATTATCAAAGGTTCCTATGATTTTCTTGCAATGAAGAATGGTGTAGCTATTGGCTCCTCGGTATGA

Protein sequence

>MsG0380012525.01.T01

MVSKPLHDPLGHLLSGWASIGPPAIRFPLSGHPPLCTRTKPIVLVVGPPAIRFSLSGHPPFMFTNQANSAGQGKGAVNPMGVKFYNNVINEVLANGLIPFVTLFHWDLPRALEDEYKGFLSLKVVKDFEAYADFCFKTFGDRVKHWVTLNEPVSYTINGYHGGTSPPARCSKYVGNCSTGDSTTEPYIVAHHFILSHAAAAKLYKAKYQAHQKGKIGITLITHYYEPYSNSVADHKAASRALDFLFGWFAHPITYGHYPQSIILSLGNRLPKFTKEESKIIKGSYDFLAMKNGVAIGSSV*