AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380012645.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380012645.01.T01 MTR_8g011350 90.761 184 10 1 12 188 211 394 2.08e-118 346
MsG0380012645.01.T01 MTR_8g011350 90.761 184 10 1 12 188 211 394 2.21e-118 346
MsG0380012645.01.T01 MTR_7g051430 59.064 171 67 3 12 180 210 379 1.55e-65 208
MsG0380012645.01.T01 MTR_7g051430 59.064 171 67 3 12 180 210 379 2.02e-64 208
MsG0380012645.01.T01 MTR_7g080180 50.515 194 86 4 12 197 210 401 5.30e-59 193
MsG0380012645.01.T01 MTR_7g080180 50.515 194 86 4 12 197 210 401 1.27e-58 192
MsG0380012645.01.T01 MTR_2g018630 28.655 171 83 6 12 180 112 245 1.39e-13 69.3
MsG0380012645.01.T01 MTR_2g018630 28.655 171 83 6 12 180 217 350 1.55e-13 69.7
MsG0380012645.01.T01 MTR_2g018590 43.421 76 40 2 12 85 214 288 4.92e-13 68.2
MsG0380012645.01.T01 MTR_0024s0320 82.857 35 6 0 12 46 39 73 5.49e-13 65.1
MsG0380012645.01.T01 MTR_4g021520 31.579 171 81 6 12 180 220 356 4.39e-12 65.1
MsG0380012645.01.T01 MTR_4g021520 44.000 75 39 2 12 84 220 293 5.08e-12 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380012645.01.T01 AT4G12910 59.146 164 65 1 12 175 215 376 2.24e-64 207
MsG0380012645.01.T01 AT4G12910 59.146 164 65 1 12 175 215 376 3.89e-64 207
MsG0380012645.01.T01 AT3G25420 54.545 176 74 2 12 183 208 381 4.13e-60 196
MsG0380012645.01.T01 AT2G22920 39.785 93 54 1 12 102 26 118 3.10e-14 70.5
MsG0380012645.01.T01 AT2G22920 39.785 93 54 1 12 102 205 297 5.66e-14 70.1
MsG0380012645.01.T01 AT2G22920 26.222 225 137 6 12 229 205 407 8.18e-14 70.1
MsG0380012645.01.T01 AT2G22920 39.785 93 54 1 12 102 205 297 8.49e-14 70.5
MsG0380012645.01.T01 AT5G09640 48.649 74 36 1 12 83 207 280 1.28e-13 69.7
MsG0380012645.01.T01 AT1G73270 39.785 93 48 2 12 99 213 302 2.00e-12 66.2
MsG0380012645.01.T01 AT1G73270 38.889 90 53 1 12 99 213 302 2.12e-12 66.2
MsG0380012645.01.T01 AT2G22980 40.789 76 43 1 12 85 84 159 1.45e-11 63.2
MsG0380012645.01.T01 AT2G22980 37.778 90 54 1 12 99 201 290 2.31e-11 63.2
MsG0380012645.01.T01 AT2G22980 40.789 76 43 1 12 85 201 276 2.44e-11 63.2
MsG0380012645.01.T01 AT2G22980 37.778 90 54 1 12 99 203 292 2.84e-11 62.8
MsG0380012645.01.T01 AT2G22980 40.789 76 43 1 12 85 201 276 2.88e-11 62.8
MsG0380012645.01.T01 AT2G22970 42.105 76 41 2 12 85 205 279 9.63e-11 61.2

Find 49 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAATTGATTTAGCAGAAT+TGG 0.262392 3:-21826871 None:intergenic
AGATTATCACATCCTATATT+AGG 0.278941 3:+21826188 MsG0380012645.01.T01:CDS
ACTTTGCCTTTCAACCAATT+TGG 0.281351 3:-21826955 None:intergenic
CATCTCTCACAGGTGCTCTA+AGG 0.317256 3:-21826291 None:intergenic
CCGTCTAGTTTCCGTGAGTT+AGG 0.343796 3:+21826214 MsG0380012645.01.T01:CDS
CATCACTTCATCACCAACTT+TGG 0.376737 3:-21826916 None:intergenic
TATCTCAGATGAATTGTTTG+AGG 0.408758 3:+21825830 MsG0380012645.01.T01:CDS
AGAGTCTAAATATATAATAC+TGG 0.418253 3:-21825721 None:intergenic
ATCTCTCACAGGTGCTCTAA+GGG 0.423846 3:-21826290 None:intergenic
TGTTCCATTTGTACATGGGA+TGG 0.425923 3:+21825803 MsG0380012645.01.T01:CDS
GAAGTCACTTGAGGCCAAAT+TGG 0.431505 3:+21826941 MsG0380012645.01.T01:CDS
ACTAGACGGTAACCTAATAT+AGG 0.445194 3:-21826200 None:intergenic
GCTCTTGTTCCATTTGTACA+TGG 0.446060 3:+21825798 MsG0380012645.01.T01:CDS
GTTCCATTTGTACATGGGAT+GGG 0.456629 3:+21825804 MsG0380012645.01.T01:CDS
TTCTGCTAAATCAATTGAAA+AGG 0.460788 3:+21826875 MsG0380012645.01.T01:CDS
AGACCCATCCCATGTACAAA+TGG 0.478452 3:-21825807 None:intergenic
TCTCCTGCTGGTTACAAAGT+TGG 0.482060 3:+21825741 MsG0380012645.01.T01:CDS
GGTTGAACAATGAAGAAGTC+AGG 0.487179 3:+21826556 MsG0380012645.01.T01:CDS
GCCTCTGTGCCGTGGTAGCA+TGG 0.493000 3:-21826160 None:intergenic
AAAGAATGTTCGGTCGTGCT+TGG 0.503362 3:+21826266 MsG0380012645.01.T01:CDS
TTGAGGCCAAATTGGTTGAA+AGG 0.513337 3:+21826949 MsG0380012645.01.T01:CDS
TTGATAAGTTGTGGCCAAGT+TGG 0.517392 3:-21826322 None:intergenic
AGGTTAACCGCGAGTGTGAT+GGG 0.518035 3:+21825965 MsG0380012645.01.T01:CDS
TTTCCAACTTTGTAACCAGC+AGG 0.523091 3:-21825744 None:intergenic
GTTACAGATGAACAAATTGA+TGG 0.533753 3:+21825771 MsG0380012645.01.T01:CDS
GAGGTTAACCGCGAGTGTGA+TGG 0.533915 3:+21825964 MsG0380012645.01.T01:CDS
CCTGTGAGGAAAAGAATGTT+CGG 0.536839 3:+21826256 MsG0380012645.01.T01:CDS
GCTGGTTACAAAGTTGGAAA+TGG 0.538341 3:+21825747 MsG0380012645.01.T01:CDS
AAAGCATTAAAAGCCAAAGT+TGG 0.538383 3:+21826903 MsG0380012645.01.T01:CDS
ATATATTTAGACTCTCCTGC+TGG 0.546816 3:+21825729 MsG0380012645.01.T01:CDS
GAGATGGCAACGTACCAACT+TGG 0.557515 3:+21826308 MsG0380012645.01.T01:CDS
CTCTTGTTCCATTTGTACAT+GGG 0.559485 3:+21825799 MsG0380012645.01.T01:CDS
TTTCAGTCTCACCTAACTCA+CGG 0.568224 3:-21826225 None:intergenic
GTAAAGAAACATACTGTGCA+TGG 0.569087 3:-21826358 None:intergenic
CTTAGAGCACCTGTGAGAGA+TGG 0.577548 3:+21826292 MsG0380012645.01.T01:CDS
TTGGTTGAAAGGCAAAGTAA+AGG 0.579751 3:+21826960 MsG0380012645.01.T01:CDS
TTGAAAGGCAAAGTAAAGGT+AGG 0.581011 3:+21826964 MsG0380012645.01.T01:CDS
GGTACGTTGCCATCTCTCAC+AGG 0.581740 3:-21826301 None:intergenic
TCTTATCTGCCTCTGTGCCG+TGG 0.592423 3:-21826168 None:intergenic
TTGTTGCTATTGATAAGTTG+TGG 0.616997 3:-21826331 None:intergenic
ATGGCTCTGTTGCAAATGCA+TGG 0.623040 3:+21826535 MsG0380012645.01.T01:CDS
CAAAGTTGGTGATGAAGTGA+TGG 0.627478 3:+21826917 MsG0380012645.01.T01:CDS
CCTAACTCACGGAAACTAGA+CGG 0.643978 3:-21826214 None:intergenic
AGAAATTCCCATCACACTCG+CGG 0.644090 3:-21825972 None:intergenic
ATTCTAGAACCATGCTACCA+CGG 0.649207 3:+21826151 MsG0380012645.01.T01:CDS
GAAAGCAATTCACACTGCAG+AGG 0.668971 3:+21826578 MsG0380012645.01.T01:CDS
TCTCTCACAGGTGCTCTAAG+GGG 0.683162 3:-21826289 None:intergenic
AGTGATGGTGAAGTCACTTG+AGG 0.722649 3:+21826932 MsG0380012645.01.T01:CDS
ACCATGCTACCACGGCACAG+AGG 0.736692 3:+21826159 MsG0380012645.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAAAATTAGCATTTCTA+AGG - Chr3:21826476-21826495 None:intergenic 15.0%
!! TTATAAGAAATAAGTGAATT+TGG + Chr3:21826073-21826092 MsG0380012645.01.T01:intron 15.0%
!!! TTGATGATTCTTATTATTTT+AGG + Chr3:21826102-21826121 MsG0380012645.01.T01:intron 15.0%
!! AGAGTCTAAATATATAATAC+TGG - Chr3:21825724-21825743 None:intergenic 20.0%
!! AGTATAAAATACAATCATGA+TGG + Chr3:21826732-21826751 MsG0380012645.01.T01:intron 20.0%
!! GTATAAAATACAATCATGAT+GGG + Chr3:21826733-21826752 MsG0380012645.01.T01:intron 20.0%
!! TTATACTAAATATACATCGA+AGG - Chr3:21826719-21826738 None:intergenic 20.0%
!!! AATAGATTTTCTTTTAGACA+AGG - Chr3:21827011-21827030 None:intergenic 20.0%
!!! TATTATTTTAGGACATTGAT+GGG + Chr3:21826113-21826132 MsG0380012645.01.T01:intron 20.0%
!!! TTATTATTTTAGGACATTGA+TGG + Chr3:21826112-21826131 MsG0380012645.01.T01:intron 20.0%
! AAATCAACTAGTAGCTAATA+AGG - Chr3:21826420-21826439 None:intergenic 25.0%
! AGATTATCACATCCTATATT+AGG + Chr3:21826188-21826207 MsG0380012645.01.T01:CDS 25.0%
! GCTAATAAGGAGATATTAAA+TGG - Chr3:21826407-21826426 None:intergenic 25.0%
! TTACAAGACAATATATAGCA+AGG - Chr3:21825908-21825927 None:intergenic 25.0%
! TTCTGCTAAATCAATTGAAA+AGG + Chr3:21826875-21826894 MsG0380012645.01.T01:CDS 25.0%
!! TTCAATTGATTTAGCAGAAT+TGG - Chr3:21826874-21826893 None:intergenic 25.0%
!! TTTTGACATTCAAAGTATAG+AGG - Chr3:21826623-21826642 None:intergenic 25.0%
ATTTATTTAGCCCTTCCTAT+AGG + Chr3:21826848-21826867 MsG0380012645.01.T01:intron 30.0%
CATGACCTAACAATATCTTT+AGG + Chr3:21826512-21826531 MsG0380012645.01.T01:intron 30.0%
GGCTAAATAAATCAAACAAG+AGG - Chr3:21826840-21826859 None:intergenic 30.0%
GTTACAGATGAACAAATTGA+TGG + Chr3:21825771-21825790 MsG0380012645.01.T01:CDS 30.0%
TATCTCAGATGAATTGTTTG+AGG + Chr3:21825830-21825849 MsG0380012645.01.T01:CDS 30.0%
TGCACAATAACTATCTTTGA+AGG - Chr3:21826814-21826833 None:intergenic 30.0%
! AAAGCATTAAAAGCCAAAGT+TGG + Chr3:21826903-21826922 MsG0380012645.01.T01:CDS 30.0%
! ACCTAACAATATCTTTAGGA+TGG + Chr3:21826516-21826535 MsG0380012645.01.T01:intron 30.0%
! TTCCAAACTTGCTCTTAAAA+GGG - Chr3:21825866-21825885 None:intergenic 30.0%
! TTGTTGCTATTGATAAGTTG+TGG - Chr3:21826334-21826353 None:intergenic 30.0%
!! TAGGTCATGTTACTTGTTTT+TGG - Chr3:21826501-21826520 None:intergenic 30.0%
ACTAGACGGTAACCTAATAT+AGG - Chr3:21826203-21826222 None:intergenic 35.0%
ACTTTGCCTTTCAACCAATT+TGG - Chr3:21826958-21826977 None:intergenic 35.0%
ATATATTTAGACTCTCCTGC+TGG + Chr3:21825729-21825748 MsG0380012645.01.T01:CDS 35.0%
CAAGCTTGCAAAAATTGATG+AGG + Chr3:21826023-21826042 MsG0380012645.01.T01:CDS 35.0%
CTCTTGTTCCATTTGTACAT+GGG + Chr3:21825799-21825818 MsG0380012645.01.T01:CDS 35.0%
GCCATCCTAAAGATATTGTT+AGG - Chr3:21826520-21826539 None:intergenic 35.0%
GTAAAGAAACATACTGTGCA+TGG - Chr3:21826361-21826380 None:intergenic 35.0%
TCTGATGAAACAGAAAAGTG+TGG + Chr3:21826660-21826679 MsG0380012645.01.T01:intron 35.0%
TTGAAAGGCAAAGTAAAGGT+AGG + Chr3:21826964-21826983 MsG0380012645.01.T01:CDS 35.0%
TTGGTTGAAAGGCAAAGTAA+AGG + Chr3:21826960-21826979 MsG0380012645.01.T01:CDS 35.0%
! ATTTAGCAGAATTGGCCTAT+AGG - Chr3:21826866-21826885 None:intergenic 35.0%
! GTTCCAAACTTGCTCTTAAA+AGG - Chr3:21825867-21825886 None:intergenic 35.0%
!! TACCCTTTTAAGAGCAAGTT+TGG + Chr3:21825861-21825880 MsG0380012645.01.T01:intron 35.0%
CATCACTTCATCACCAACTT+TGG - Chr3:21826919-21826938 None:intergenic 40.0%
CCTGTGAGGAAAAGAATGTT+CGG + Chr3:21826256-21826275 MsG0380012645.01.T01:CDS 40.0%
GCTCTTGTTCCATTTGTACA+TGG + Chr3:21825798-21825817 MsG0380012645.01.T01:CDS 40.0%
GCTGGTTACAAAGTTGGAAA+TGG + Chr3:21825747-21825766 MsG0380012645.01.T01:CDS 40.0%
GGTTGAACAATGAAGAAGTC+AGG + Chr3:21826556-21826575 MsG0380012645.01.T01:CDS 40.0%
GTTCCATTTGTACATGGGAT+GGG + Chr3:21825804-21825823 MsG0380012645.01.T01:CDS 40.0%
TGTTCCATTTGTACATGGGA+TGG + Chr3:21825803-21825822 MsG0380012645.01.T01:CDS 40.0%
TTGAGGCCAAATTGGTTGAA+AGG + Chr3:21826949-21826968 MsG0380012645.01.T01:CDS 40.0%
TTGATAAGTTGTGGCCAAGT+TGG - Chr3:21826325-21826344 None:intergenic 40.0%
TTTCAGTCTCACCTAACTCA+CGG - Chr3:21826228-21826247 None:intergenic 40.0%
TTTCCAACTTTGTAACCAGC+AGG - Chr3:21825747-21825766 None:intergenic 40.0%
! AGAAAAGTGTGGTCAGTAGT+TGG + Chr3:21826671-21826690 MsG0380012645.01.T01:intron 40.0%
! ATTCTTTTCCTCACAGGATG+AGG - Chr3:21826253-21826272 None:intergenic 40.0%
! GAAAAGTGTGGTCAGTAGTT+GGG + Chr3:21826672-21826691 MsG0380012645.01.T01:intron 40.0%
!! ATTCTAGAACCATGCTACCA+CGG + Chr3:21826151-21826170 MsG0380012645.01.T01:CDS 40.0%
!! CAAAGTTGGTGATGAAGTGA+TGG + Chr3:21826917-21826936 MsG0380012645.01.T01:CDS 40.0%
AAAGAATGTTCGGTCGTGCT+TGG + Chr3:21826266-21826285 MsG0380012645.01.T01:CDS 45.0%
AGAAATTCCCATCACACTCG+CGG - Chr3:21825975-21825994 None:intergenic 45.0%
AGACCCATCCCATGTACAAA+TGG - Chr3:21825810-21825829 None:intergenic 45.0%
ATCTCTCACAGGTGCTCTAA+GGG - Chr3:21826293-21826312 None:intergenic 45.0%
CCTAACTCACGGAAACTAGA+CGG - Chr3:21826217-21826236 None:intergenic 45.0%
CTGAAAAACCTCATCCTGTG+AGG + Chr3:21826242-21826261 MsG0380012645.01.T01:CDS 45.0%
GAAAGCAATTCACACTGCAG+AGG + Chr3:21826578-21826597 MsG0380012645.01.T01:CDS 45.0%
GAAGTCACTTGAGGCCAAAT+TGG + Chr3:21826941-21826960 MsG0380012645.01.T01:CDS 45.0%
! AGCAGAATTGGCCTATAGGA+AGG - Chr3:21826862-21826881 None:intergenic 45.0%
! AGTGATGGTGAAGTCACTTG+AGG + Chr3:21826932-21826951 MsG0380012645.01.T01:CDS 45.0%
! ATGGCTCTGTTGCAAATGCA+TGG + Chr3:21826535-21826554 MsG0380012645.01.T01:CDS 45.0%
! CCGAACATTCTTTTCCTCAC+AGG - Chr3:21826259-21826278 None:intergenic 45.0%
! GCAGAATTGGCCTATAGGAA+GGG - Chr3:21826861-21826880 None:intergenic 45.0%
! TCTCCTGCTGGTTACAAAGT+TGG + Chr3:21825741-21825760 MsG0380012645.01.T01:CDS 45.0%
AGGTTAACCGCGAGTGTGAT+GGG + Chr3:21825965-21825984 MsG0380012645.01.T01:CDS 50.0%
ATGTTGCACTCTGTTGCAGG+AGG + Chr3:21825945-21825964 MsG0380012645.01.T01:intron 50.0%
CAATTCACACTGCAGAGGTG+AGG + Chr3:21826583-21826602 MsG0380012645.01.T01:intron 50.0%
CATCTCTCACAGGTGCTCTA+AGG - Chr3:21826294-21826313 None:intergenic 50.0%
GAGATGGCAACGTACCAACT+TGG + Chr3:21826308-21826327 MsG0380012645.01.T01:CDS 50.0%
GTCATGTTGCACTCTGTTGC+AGG + Chr3:21825942-21825961 MsG0380012645.01.T01:intron 50.0%
! CCGTCTAGTTTCCGTGAGTT+AGG + Chr3:21826214-21826233 MsG0380012645.01.T01:CDS 50.0%
! CTTAGAGCACCTGTGAGAGA+TGG + Chr3:21826292-21826311 MsG0380012645.01.T01:CDS 50.0%
! TCTCTCACAGGTGCTCTAAG+GGG - Chr3:21826292-21826311 None:intergenic 50.0%
GAGGTTAACCGCGAGTGTGA+TGG + Chr3:21825964-21825983 MsG0380012645.01.T01:CDS 55.0%
GGTACGTTGCCATCTCTCAC+AGG - Chr3:21826304-21826323 None:intergenic 55.0%
TCTTATCTGCCTCTGTGCCG+TGG - Chr3:21826171-21826190 None:intergenic 55.0%
ACCATGCTACCACGGCACAG+AGG + Chr3:21826159-21826178 MsG0380012645.01.T01:CDS 60.0%
GCCTCTGTGCCGTGGTAGCA+TGG - Chr3:21826163-21826182 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr3 gene 21825717 21827040 21825717 ID=MsG0380012645.01;Name=MsG0380012645.01
Chr3 mRNA 21825717 21827040 21825717 ID=MsG0380012645.01.T01;Parent=MsG0380012645.01;Name=MsG0380012645.01.T01;_AED=0.29;_eAED=0.29;_QI=0|0|0|0.6|1|1|5|0|231
Chr3 exon 21825717 21825851 21825717 ID=MsG0380012645.01.T01:exon:7982;Parent=MsG0380012645.01.T01
Chr3 exon 21825964 21826044 21825964 ID=MsG0380012645.01.T01:exon:7983;Parent=MsG0380012645.01.T01
Chr3 exon 21826124 21826366 21826124 ID=MsG0380012645.01.T01:exon:7984;Parent=MsG0380012645.01.T01
Chr3 exon 21826534 21826599 21826534 ID=MsG0380012645.01.T01:exon:7985;Parent=MsG0380012645.01.T01
Chr3 exon 21826870 21827040 21826870 ID=MsG0380012645.01.T01:exon:7986;Parent=MsG0380012645.01.T01
Chr3 CDS 21825717 21825851 21825717 ID=MsG0380012645.01.T01:cds;Parent=MsG0380012645.01.T01
Chr3 CDS 21825964 21826044 21825964 ID=MsG0380012645.01.T01:cds;Parent=MsG0380012645.01.T01
Chr3 CDS 21826124 21826366 21826124 ID=MsG0380012645.01.T01:cds;Parent=MsG0380012645.01.T01
Chr3 CDS 21826534 21826599 21826534 ID=MsG0380012645.01.T01:cds;Parent=MsG0380012645.01.T01
Chr3 CDS 21826870 21827040 21826870 ID=MsG0380012645.01.T01:cds;Parent=MsG0380012645.01.T01
Gene Sequence

>MsG0380012645.01.T01

GTTTCCAGTATTATATATTTAGACTCTCCTGCTGGTTACAAAGTTGGAAATGGTGTTACAGATGAACAAATTGATGGCAATGCTCTTGTTCCATTTGTACATGGGATGGGTCTTATCTCAGATGAATTGTTTGAGGAGGTTAACCGCGAGTGTGATGGGAATTTCTATAATTCACTCAGTGATAATTGTACAAACAAGCTTGCAAAAATTGATGAGGACATTGATGGGTTGAATGTATATAACATTCTAGAACCATGCTACCACGGCACAGAGGCAGATAAGATTATCACATCCTATATTAGGTTACCGTCTAGTTTCCGTGAGTTAGGTGAGACTGAAAAACCTCATCCTGTGAGGAAAAGAATGTTCGGTCGTGCTTGGCCCCTTAGAGCACCTGTGAGAGATGGCAACGTACCAACTTGGCCACAACTTATCAATAGCAACAATGTTCCATGCACAGATGGCTCTGTTGCAAATGCATGGTTGAACAATGAAGAAGTCAGGAAAGCAATTCACACTGCAGAGGCCAATTCTGCTAAATCAATTGAAAAGGATCATAAAGCATTAAAAGCCAAAGTTGGTGATGAAGTGATGGTGAAGTCACTTGAGGCCAAATTGGTTGAAAGGCAAAGTAAAGGTAGGTTCTTGTGCAATATTGTTATACCTTGTCTAAAAGAAAATCTATTTTCTGACTGA

Protein sequence

>MsG0380012645.01.T01

VSSIIYLDSPAGYKVGNGVTDEQIDGNALVPFVHGMGLISDELFEEVNRECDGNFYNSLSDNCTNKLAKIDEDIDGLNVYNILEPCYHGTEADKIITSYIRLPSSFRELGETEKPHPVRKRMFGRAWPLRAPVRDGNVPTWPQLINSNNVPCTDGSVANAWLNNEEVRKAIHTAEANSAKSIEKDHKALKAKVGDEVMVKSLEAKLVERQSKGRFLCNIVIPCLKENLFSD*