AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780036201.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780036201.01.T01 MTR_7g062720 39.773 88 47 2 1 87 1 83 1.92e-15 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 20 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTATTATTGCCCTCATTAA+TGG 0.192737 7:+4633497 MsG0780036201.01.T01:CDS
CAAAAGATCCCAAGATCTTA+TGG 0.237847 7:+4633440 MsG0780036201.01.T01:CDS
GATTCTTGAGGGAGACGATT+TGG 0.271296 7:+4633321 MsG0780036201.01.T01:CDS
CTCAATTAAGTTGTTGTTCC+AGG 0.274617 7:-4633272 None:intergenic
GGACAAATTAAAGCAAGATT+TGG 0.312406 7:+4633462 MsG0780036201.01.T01:CDS
TAATTTGTCCCATAAGATCT+TGG 0.340378 7:-4633449 None:intergenic
ATTCTTGAGGGAGACGATTT+GGG 0.354692 7:+4633322 MsG0780036201.01.T01:CDS
AATTTGTCCCATAAGATCTT+GGG 0.416721 7:-4633448 None:intergenic
AAAAGATCCCAAGATCTTAT+GGG 0.458748 7:+4633441 MsG0780036201.01.T01:CDS
GAGGGAGACGATTTGGGAGA+TGG 0.461669 7:+4633328 MsG0780036201.01.T01:CDS
ATCAAGCACCACATTGATGA+TGG 0.492315 7:+4633394 MsG0780036201.01.T01:CDS
CTGGCGACAACTACATAACC+TGG 0.502097 7:+4633254 MsG0780036201.01.T01:CDS
GTTCCATTGATCCATTAATG+AGG 0.509644 7:-4633508 None:intergenic
GGACTAGACAAGATTCTTGA+GGG 0.521095 7:+4633310 MsG0780036201.01.T01:CDS
TTCCATTGATCCATTAATGA+GGG 0.533283 7:-4633507 None:intergenic
AATTGAGTATCTTGCATGTG+AGG 0.565013 7:+4633288 MsG0780036201.01.T01:CDS
ATTGAGTATCTTGCATGTGA+GGG 0.575966 7:+4633289 MsG0780036201.01.T01:CDS
GGGACTAGACAAGATTCTTG+AGG 0.613207 7:+4633309 MsG0780036201.01.T01:CDS
CGATTTGGGAGATGGCCCAG+AGG 0.681880 7:+4633336 MsG0780036201.01.T01:CDS
TTTGTAATCCATCATCAATG+TGG 0.715751 7:-4633402 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTCAAAATTTTAAGCATAAC+TGG + Chr7:4633235-4633254 MsG0780036201.01.T01:CDS 20.0%
AAAAGATCCCAAGATCTTAT+GGG + Chr7:4633441-4633460 MsG0780036201.01.T01:CDS 30.0%
AATTTGTCCCATAAGATCTT+GGG - Chr7:4633451-4633470 None:intergenic 30.0%
GGACAAATTAAAGCAAGATT+TGG + Chr7:4633462-4633481 MsG0780036201.01.T01:CDS 30.0%
TTTGTAATCCATCATCAATG+TGG - Chr7:4633405-4633424 None:intergenic 30.0%
! TAATTTGTCCCATAAGATCT+TGG - Chr7:4633452-4633471 None:intergenic 30.0%
! TTTTCTTTGCTAATTCCTCT+GGG - Chr7:4633354-4633373 None:intergenic 30.0%
! TTTTTCTTTGCTAATTCCTC+TGG - Chr7:4633355-4633374 None:intergenic 30.0%
AATTGAGTATCTTGCATGTG+AGG + Chr7:4633288-4633307 MsG0780036201.01.T01:CDS 35.0%
ATTGAGTATCTTGCATGTGA+GGG + Chr7:4633289-4633308 MsG0780036201.01.T01:CDS 35.0%
CAAAAGATCCCAAGATCTTA+TGG + Chr7:4633440-4633459 MsG0780036201.01.T01:CDS 35.0%
! CTCAATTAAGTTGTTGTTCC+AGG - Chr7:4633275-4633294 None:intergenic 35.0%
ATCAAGCACCACATTGATGA+TGG + Chr7:4633394-4633413 MsG0780036201.01.T01:CDS 40.0%
ATTCTTGAGGGAGACGATTT+GGG + Chr7:4633322-4633341 MsG0780036201.01.T01:CDS 40.0%
GGACTAGACAAGATTCTTGA+GGG + Chr7:4633310-4633329 MsG0780036201.01.T01:CDS 40.0%
GATTCTTGAGGGAGACGATT+TGG + Chr7:4633321-4633340 MsG0780036201.01.T01:CDS 45.0%
GGGACTAGACAAGATTCTTG+AGG + Chr7:4633309-4633328 MsG0780036201.01.T01:CDS 45.0%
CTGGCGACAACTACATAACC+TGG + Chr7:4633254-4633273 MsG0780036201.01.T01:CDS 50.0%
! GAGGGAGACGATTTGGGAGA+TGG + Chr7:4633328-4633347 MsG0780036201.01.T01:CDS 55.0%
! CGATTTGGGAGATGGCCCAG+AGG + Chr7:4633336-4633355 MsG0780036201.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr7 gene 4633214 4633516 4633214 ID=MsG0780036201.01;Name=MsG0780036201.01
Chr7 mRNA 4633214 4633516 4633214 ID=MsG0780036201.01.T01;Parent=MsG0780036201.01;Name=MsG0780036201.01.T01;_AED=0.46;_eAED=0.47;_QI=0|-1|0|1|-1|1|1|0|100
Chr7 exon 4633214 4633516 4633214 ID=MsG0780036201.01.T01:exon:2588;Parent=MsG0780036201.01.T01
Chr7 CDS 4633214 4633516 4633214 ID=MsG0780036201.01.T01:cds;Parent=MsG0780036201.01.T01
Gene Sequence

>MsG0780036201.01.T01

ATGTCAAACGTTAAGCATCAATTCAAAATTTTAAGCATAACTGGCGACAACTACATAACCTGGAACAACAACTTAATTGAGTATCTTGCATGTGAGGGACTAGACAAGATTCTTGAGGGAGACGATTTGGGAGATGGCCCAGAGGAATTAGCAAAGAAAAAATCAAAAGTAAATCGTATAATCAAGCACCACATTGATGATGGATTACAAACAGAGTATTCAAATGCAAAAGATCCCAAGATCTTATGGGACAAATTAAAGCAAGATTTGGACATCAGAAGAAAGTATTATTGCCCTCATTAA

Protein sequence

>MsG0780036201.01.T01

MSNVKHQFKILSITGDNYITWNNNLIEYLACEGLDKILEGDDLGDGPEELAKKKSKVNRIIKHHIDDGLQTEYSNAKDPKILWDKLKQDLDIRRKYYCPH*