Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013569.01.T02 | XP_024634540.1 | 63.934 | 61 | 22 | 0 | 2 | 62 | 198 | 258 | 1.02E-14 | 76.6 |
| MsG0380013569.01.T01 | XP_024641784.2 | 62.308 | 130 | 49 | 0 | 1 | 130 | 72 | 201 | 1.53E-48 | 165 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013569.01.T01 | A0A2Z6NW35 | 60.150 | 133 | 53 | 0 | 1 | 133 | 263 | 395 | 3.49e-48 | 169 |
| MsG0380013569.01.T02 | A0A2Z6NHQ6 | 61.290 | 62 | 24 | 0 | 1 | 62 | 924 | 985 | 4.94e-15 | 77.8 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0780041116.01 | MsG0380013569.01 | PPI |
| MsG0780041116.01 | MsG0380013569.01 | PPI |
| MsG0380013569.01 | MsG0280010449.01 | PPI |
| MsG0380013578.01 | MsG0380013569.01 | PPI |
| MsG0780040274.01 | MsG0380013569.01 | PPI |
| MsG0280009322.01 | MsG0380013569.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013569.01.T02 | MTR_4g036855 | 52.727 | 55 | 26 | 0 | 5 | 59 | 34 | 88 | 1.69e-13 | 59.7 |
| MsG0380013569.01.T01 | MTR_7g063280 | 72.165 | 97 | 27 | 0 | 1 | 97 | 78 | 174 | 1.05e-45 | 147 |
| MsG0380013569.01.T01 | MTR_6g016135 | 81.034 | 58 | 11 | 0 | 1 | 58 | 385 | 442 | 8.30e-27 | 103 |
| MsG0380013569.01.T01 | MTR_1g045560 | 46.364 | 110 | 43 | 2 | 1 | 110 | 1 | 94 | 9.13e-27 | 96.3 |
| MsG0380013569.01.T01 | MTR_1g074090 | 73.333 | 60 | 16 | 0 | 1 | 60 | 87 | 146 | 7.82e-26 | 95.5 |
| MsG0380013569.01.T01 | MTR_7g053400 | 44.954 | 109 | 26 | 1 | 1 | 109 | 181 | 255 | 3.93e-25 | 96.3 |
| MsG0380013569.01.T01 | MTR_6g016265 | 52.500 | 80 | 38 | 0 | 1 | 80 | 45 | 124 | 5.43e-25 | 92.8 |
| MsG0380013569.01.T01 | MTR_7g072030 | 81.481 | 54 | 10 | 0 | 15 | 68 | 69 | 122 | 7.08e-25 | 92.4 |
| MsG0380013569.01.T01 | MTR_0015s0120 | 64.706 | 68 | 24 | 0 | 1 | 68 | 299 | 366 | 2.41e-24 | 96.3 |
| MsG0380013569.01.T01 | MTR_5g049680 | 57.746 | 71 | 29 | 1 | 1 | 70 | 37 | 107 | 9.41e-24 | 89.0 |
| MsG0380013569.01.T01 | MTR_2g027020 | 36.923 | 130 | 78 | 1 | 1 | 130 | 34 | 159 | 1.40e-23 | 90.9 |
| MsG0380013569.01.T01 | MTR_4g036855 | 45.217 | 115 | 35 | 1 | 16 | 130 | 2 | 88 | 1.42e-23 | 88.2 |
| MsG0380013569.01.T01 | MTR_6g034915 | 34.586 | 133 | 70 | 1 | 1 | 133 | 61 | 176 | 2.57e-22 | 87.4 |
| MsG0380013569.01.T01 | MTR_5g075610 | 40.000 | 100 | 58 | 1 | 1 | 100 | 1 | 98 | 1.56e-18 | 75.5 |
| MsG0380013569.01.T01 | MTR_3g026270 | 56.604 | 53 | 23 | 0 | 3 | 55 | 3 | 55 | 1.99e-18 | 74.3 |
| MsG0380013569.01.T01 | MTR_7g013520 | 33.083 | 133 | 64 | 1 | 1 | 133 | 76 | 183 | 3.82e-18 | 76.6 |
| MsG0380013569.01.T01 | MTR_5g091440 | 35.714 | 126 | 48 | 1 | 3 | 128 | 3 | 95 | 2.43e-17 | 73.2 |
| MsG0380013569.01.T01 | MTR_1g069475 | 46.575 | 73 | 39 | 0 | 2 | 74 | 171 | 243 | 7.85e-17 | 74.3 |
| MsG0380013569.01.T01 | MTR_3g465840 | 38.938 | 113 | 67 | 1 | 21 | 133 | 1 | 111 | 1.25e-16 | 72.0 |
| MsG0380013569.01.T01 | MTR_6g453270 | 44.595 | 74 | 41 | 0 | 1 | 74 | 306 | 379 | 3.11e-16 | 73.9 |
| MsG0380013569.01.T01 | MTR_8g088220 | 42.105 | 76 | 44 | 0 | 5 | 80 | 4 | 79 | 3.76e-16 | 68.6 |
| MsG0380013569.01.T01 | MTR_8g098925 | 39.130 | 92 | 56 | 0 | 1 | 92 | 71 | 162 | 5.17e-16 | 70.5 |
| MsG0380013569.01.T01 | MTR_6g089560 | 31.579 | 133 | 69 | 1 | 1 | 133 | 122 | 232 | 5.47e-16 | 72.0 |
| MsG0380013569.01.T01 | MTR_6g013970 | 41.892 | 74 | 43 | 0 | 3 | 76 | 107 | 180 | 6.76e-16 | 71.2 |
| MsG0380013569.01.T01 | MTR_2g059470 | 42.667 | 75 | 43 | 0 | 1 | 75 | 6 | 80 | 1.85e-15 | 67.0 |
| MsG0380013569.01.T01 | MTR_4g008270 | 38.462 | 78 | 48 | 0 | 1 | 78 | 16 | 93 | 3.63e-15 | 66.6 |
| MsG0380013569.01.T01 | MTR_1g088650 | 38.776 | 98 | 60 | 0 | 1 | 98 | 92 | 189 | 3.76e-15 | 68.9 |
| MsG0380013569.01.T01 | MTR_5g083200 | 43.056 | 72 | 41 | 0 | 3 | 74 | 106 | 177 | 9.79e-15 | 68.2 |
| MsG0380013569.01.T01 | MTR_0002s1220 | 43.056 | 72 | 41 | 0 | 3 | 74 | 106 | 177 | 9.79e-15 | 68.2 |
| MsG0380013569.01.T01 | MTR_3g111390 | 43.056 | 72 | 41 | 0 | 3 | 74 | 106 | 177 | 2.44e-14 | 67.0 |
| MsG0380013569.01.T01 | MTR_1g070820 | 43.056 | 72 | 41 | 0 | 3 | 74 | 106 | 177 | 2.44e-14 | 67.0 |
| MsG0380013569.01.T01 | MTR_0093s0070 | 41.892 | 74 | 43 | 0 | 1 | 74 | 129 | 202 | 3.17e-14 | 66.6 |
| MsG0380013569.01.T01 | MTR_1g045340 | 41.667 | 72 | 42 | 0 | 3 | 74 | 92 | 163 | 3.46e-14 | 66.2 |
| MsG0380013569.01.T01 | MTR_1g081930 | 37.363 | 91 | 50 | 1 | 1 | 91 | 44 | 127 | 4.51e-14 | 64.7 |
| MsG0380013569.01.T01 | MTR_1g034430 | 37.037 | 81 | 51 | 0 | 1 | 81 | 101 | 181 | 1.36e-13 | 64.7 |
| MsG0380013569.01.T01 | MTR_8g099580 | 44.595 | 74 | 41 | 0 | 1 | 74 | 212 | 285 | 1.58e-13 | 66.2 |
| MsG0380013569.01.T01 | MTR_1g074240 | 44.615 | 65 | 36 | 0 | 1 | 65 | 6 | 70 | 2.09e-13 | 61.6 |
| MsG0380013569.01.T01 | MTR_5g037920 | 34.146 | 123 | 79 | 2 | 1 | 122 | 104 | 225 | 2.50e-13 | 64.7 |
| MsG0380013569.01.T01 | MTR_3g116210 | 39.189 | 74 | 45 | 0 | 1 | 74 | 103 | 176 | 4.43e-13 | 63.5 |
| MsG0380013569.01.T01 | MTR_3g010950 | 39.474 | 76 | 46 | 0 | 55 | 130 | 56 | 131 | 4.72e-13 | 62.4 |
| MsG0380013569.01.T01 | MTR_1g017840 | 43.836 | 73 | 41 | 0 | 1 | 73 | 84 | 156 | 1.21e-12 | 61.6 |
| MsG0380013569.01.T01 | MTR_5g014420 | 54.545 | 44 | 20 | 0 | 32 | 75 | 72 | 115 | 1.36e-12 | 60.5 |
| MsG0380013569.01.T01 | MTR_8g031080 | 31.538 | 130 | 63 | 2 | 1 | 130 | 295 | 398 | 1.57e-12 | 63.5 |
| MsG0380013569.01.T01 | MTR_8g031800 | 36.111 | 72 | 46 | 0 | 1 | 72 | 37 | 108 | 3.10e-12 | 59.7 |
| MsG0380013569.01.T01 | MTR_5g069700 | 38.028 | 71 | 44 | 0 | 16 | 86 | 2 | 72 | 4.37e-12 | 58.2 |
| MsG0380013569.01.T01 | MTR_1g050488 | 49.123 | 57 | 29 | 0 | 2 | 58 | 105 | 161 | 5.56e-12 | 60.1 |
| MsG0380013569.01.T01 | MTR_4g098670 | 37.975 | 79 | 49 | 0 | 50 | 128 | 16 | 94 | 9.13e-12 | 57.8 |
| MsG0380013569.01.T01 | MTR_4g029360 | 34.409 | 93 | 61 | 0 | 3 | 95 | 135 | 227 | 1.20e-11 | 60.1 |
| MsG0380013569.01.T01 | MTR_1g052310 | 28.829 | 111 | 79 | 0 | 26 | 136 | 17 | 127 | 1.48e-11 | 58.2 |
| MsG0380013569.01.T01 | MTR_5g034450 | 44.828 | 58 | 32 | 0 | 1 | 58 | 55 | 112 | 4.59e-11 | 56.6 |
| MsG0380013569.01.T01 | MTR_5g085210 | 32.727 | 110 | 58 | 2 | 1 | 110 | 125 | 218 | 4.83e-11 | 58.5 |
| MsG0380013569.01.T01 | MTR_4g073820 | 51.852 | 54 | 24 | 1 | 1 | 54 | 1 | 52 | 6.94e-11 | 55.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 24 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGTCAGGATGTTACAAAATT+TGG | 0.071391 | 3:-40901798 | MsG0380013569.01.T01:CDS |
| TGTGAATAGAAGTTTGTATA+AGG | 0.255510 | 3:-40901998 | None:intergenic |
| TGGCCTCCTTAGCTAATCTA+TGG | 0.292383 | 3:+40901685 | MsG0380013568.01.T01:intron |
| TAAACTATCCACCACTTCTT+TGG | 0.316701 | 3:+40901830 | MsG0380013568.01.T01:intron |
| GTCAGGATGTTACAAAATTT+GGG | 0.354377 | 3:-40901797 | MsG0380013569.01.T01:CDS |
| GTACATGTTGGCAAAGCTAT+TGG | 0.355428 | 3:-40901918 | MsG0380013569.01.T01:CDS |
| TATTGGATTGTTATTAGCTT+TGG | 0.358538 | 3:-40901901 | MsG0380013569.01.T01:CDS |
| AAAATGTGTTTAATACAATC+TGG | 0.417334 | 3:+40901627 | MsG0380013568.01.T01:intron |
| AGCTAAGGAGGCCACATCTT+CGG | 0.431350 | 3:-40901676 | MsG0380013569.01.T01:CDS |
| TAGTTTATCATCTGCACGTC+AGG | 0.445980 | 3:-40901814 | MsG0380013569.01.T01:CDS |
| GTGAATAGAAGTTTGTATAA+GGG | 0.505540 | 3:-40901997 | None:intergenic |
| TGTGAGGAGACATGCAAATG+AGG | 0.555190 | 3:-40901712 | MsG0380013569.01.T01:CDS |
| ATGTGTTCATCAATTACACT+TGG | 0.566231 | 3:-40901877 | MsG0380013569.01.T01:CDS |
| ACTTTAGTGTTGAGTTTGTG+AGG | 0.568252 | 3:-40901728 | MsG0380013569.01.T01:CDS |
| GGCATCCCATAGATTAGCTA+AGG | 0.579785 | 3:-40901691 | MsG0380013569.01.T01:CDS |
| CTGAAAACTAGCCGAAGATG+TGG | 0.586945 | 3:+40901665 | MsG0380013568.01.T01:intron |
| CAAGTCTGTGAGGTACATGT+TGG | 0.597463 | 3:-40901930 | MsG0380013569.01.T01:CDS |
| GGCCTCCTTAGCTAATCTAT+GGG | 0.615022 | 3:+40901686 | MsG0380013568.01.T01:intron |
| ATCCCATAGATTAGCTAAGG+AGG | 0.615553 | 3:-40901688 | MsG0380013569.01.T01:CDS |
| GCATCAAGCTCAAAATCGAT+AGG | 0.619848 | 3:+40901852 | MsG0380013568.01.T01:intron |
| TGTGTTCATCAATTACACTT+GGG | 0.623250 | 3:-40901876 | MsG0380013569.01.T01:CDS |
| TGAGCTTGATGCCAAAGAAG+TGG | 0.646305 | 3:-40901841 | MsG0380013569.01.T01:CDS |
| GCTTGATGCCAAAGAAGTGG+TGG | 0.669926 | 3:-40901838 | MsG0380013569.01.T01:CDS |
| ATTGTTTACTCAAGTCTGTG+AGG | 0.685113 | 3:-40901940 | MsG0380013569.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTGGATTGTTATAAAATAT+AGG | - | Chr3:40901988-40902007 | MsG0380013569.01.T01:CDS | 15.0% |
| !!! | CAATGATAGTTTTATAATTA+CGG | + | Chr3:40901817-40901836 | MsG0380013568.01.T01:intron | 15.0% |
| !! | AAAATGTGTTTAATACAATC+TGG | + | Chr3:40901956-40901975 | MsG0380013568.01.T01:intron | 20.0% |
| !!! | CATTTTAAGTAATACAAGAT+TGG | - | Chr3:40901970-40901989 | MsG0380013569.01.T01:CDS | 20.0% |
| !! | TATTGGATTGTTATTAGCTT+TGG | - | Chr3:40901679-40901698 | MsG0380013569.01.T01:CDS | 25.0% |
| !!! | TGATACAAACACTTTTGTTT+TGG | - | Chr3:40901607-40901626 | MsG0380013569.01.T01:CDS | 25.0% |
| ATGTGTTCATCAATTACACT+TGG | - | Chr3:40901703-40901722 | MsG0380013569.01.T01:CDS | 30.0% | |
| GTCAGGATGTTACAAAATTT+GGG | - | Chr3:40901783-40901802 | MsG0380013569.01.T01:CDS | 30.0% | |
| TGTGTTCATCAATTACACTT+GGG | - | Chr3:40901704-40901723 | MsG0380013569.01.T01:CDS | 30.0% | |
| ATTGTTTACTCAAGTCTGTG+AGG | - | Chr3:40901640-40901659 | MsG0380013569.01.T01:CDS | 35.0% | |
| CGTCAGGATGTTACAAAATT+TGG | - | Chr3:40901782-40901801 | MsG0380013569.01.T01:CDS | 35.0% | |
| TAAACTATCCACCACTTCTT+TGG | + | Chr3:40901753-40901772 | MsG0380013568.01.T01:intron | 35.0% | |
| ! | ACTTTAGTGTTGAGTTTGTG+AGG | - | Chr3:40901852-40901871 | MsG0380013569.01.T01:CDS | 35.0% |
| !!! | TTCGGCTAGTTTTCAGATTT+TGG | - | Chr3:40901922-40901941 | MsG0380013569.01.T01:CDS | 35.0% |
| GCATCAAGCTCAAAATCGAT+AGG | + | Chr3:40901731-40901750 | MsG0380013568.01.T01:intron | 40.0% | |
| GTACATGTTGGCAAAGCTAT+TGG | - | Chr3:40901662-40901681 | MsG0380013569.01.T01:CDS | 40.0% | |
| TAGTTTATCATCTGCACGTC+AGG | - | Chr3:40901766-40901785 | MsG0380013569.01.T01:CDS | 40.0% | |
| ! | ATCCCATAGATTAGCTAAGG+AGG | - | Chr3:40901892-40901911 | MsG0380013569.01.T01:CDS | 40.0% |
| CAAGTCTGTGAGGTACATGT+TGG | - | Chr3:40901650-40901669 | MsG0380013569.01.T01:CDS | 45.0% | |
| CTGAAAACTAGCCGAAGATG+TGG | + | Chr3:40901918-40901937 | MsG0380013568.01.T01:intron | 45.0% | |
| GGCCTCCTTAGCTAATCTAT+GGG | + | Chr3:40901897-40901916 | MsG0380013568.01.T01:intron | 45.0% | |
| TGAGCTTGATGCCAAAGAAG+TGG | - | Chr3:40901739-40901758 | MsG0380013569.01.T01:CDS | 45.0% | |
| TGGCCTCCTTAGCTAATCTA+TGG | + | Chr3:40901898-40901917 | MsG0380013568.01.T01:intron | 45.0% | |
| TGTGAGGAGACATGCAAATG+AGG | - | Chr3:40901868-40901887 | MsG0380013569.01.T01:CDS | 45.0% | |
| ! | GGCATCCCATAGATTAGCTA+AGG | - | Chr3:40901889-40901908 | MsG0380013569.01.T01:CDS | 45.0% |
| AGCTAAGGAGGCCACATCTT+CGG | - | Chr3:40901904-40901923 | MsG0380013569.01.T01:CDS | 50.0% | |
| GCTTGATGCCAAAGAAGTGG+TGG | - | Chr3:40901742-40901761 | MsG0380013569.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 40901593 | 40902009 | 40901593 | ID=MsG0380013569.01;Name=MsG0380013569.01 |
| Chr3 | mRNA | 40901593 | 40902009 | 40901593 | ID=MsG0380013569.01.T01;Parent=MsG0380013569.01;Name=MsG0380013569.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|0|1|0|138 |
| Chr3 | exon | 40901593 | 40902009 | 40901593 | ID=MsG0380013569.01.T01:exon:1624;Parent=MsG0380013569.01.T01 |
| Chr3 | CDS | 40901593 | 40902009 | 40901593 | ID=MsG0380013569.01.T01:cds;Parent=MsG0380013569.01.T01 |
| Chr3 | mRNA | 40901593 | 40901796 | 40901593 | ID=MsG0380013569.01.T02;Parent=MsG0380013569.01;Name=MsG0380013569.01.T02;_AED=0.53;_eAED=0.53;_QI=0|-1|0|1|-1|0|1|0|67 |
| Chr3 | exon | 40901593 | 40901796 | 40901593 | ID=MsG0380013569.01.T02:exon:1625;Parent=MsG0380013569.01.T02 |
| Chr3 | CDS | 40901593 | 40901796 | 40901593 | ID=MsG0380013569.01.T02:cds;Parent=MsG0380013569.01.T02 |
| Gene Sequence |
| Protein sequence |
