Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013621.01.T01 | AES97196.2 | 72.034 | 118 | 28 | 2 | 8 | 123 | 29 | 143 | 1.19E-47 | 160 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180004389.01 | MsG0380013621.01 | 0.804032 | 2.623750e-49 | 1.338824e-46 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013621.01.T01 | MTR_5g046830 | 72.034 | 118 | 28 | 2 | 8 | 123 | 29 | 143 | 1.45e-51 | 160 |
| MsG0380013621.01.T01 | MTR_6g015975 | 41.026 | 117 | 52 | 3 | 17 | 123 | 109 | 218 | 3.43e-18 | 77.4 |
| MsG0380013621.01.T01 | MTR_7g016600 | 39.200 | 125 | 60 | 4 | 8 | 123 | 100 | 217 | 5.09e-17 | 74.3 |
| MsG0380013621.01.T01 | MTR_4g109810 | 38.462 | 117 | 55 | 3 | 17 | 123 | 108 | 217 | 1.54e-15 | 70.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013621.01.T01 | AT5G15800 | 38.281 | 128 | 60 | 3 | 8 | 123 | 101 | 221 | 6.11e-15 | 68.9 |
| MsG0380013621.01.T01 | AT5G15800 | 38.281 | 128 | 60 | 3 | 8 | 123 | 101 | 221 | 8.55e-15 | 68.6 |
| MsG0380013621.01.T01 | AT2G03710 | 36.170 | 141 | 61 | 6 | 5 | 122 | 94 | 228 | 7.29e-14 | 66.2 |
| MsG0380013621.01.T01 | AT2G03710 | 36.170 | 141 | 61 | 6 | 5 | 122 | 94 | 228 | 7.29e-14 | 66.2 |
| MsG0380013621.01.T01 | AT3G02310 | 33.858 | 127 | 66 | 4 | 8 | 123 | 101 | 220 | 4.25e-13 | 63.9 |
Find 1431 sgRNAs with CRISPR-Local
Find 2156 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACAATAGAGAGAGAAAA+AGG | 0.345433 | 3:+42015550 | None:intergenic |
| AAAACAATAGAGAGAGAAAA+AGG | 0.345433 | 3:+42015665 | None:intergenic |
| AAAACCTCCGGGGCAGCGGG+GGG | 0.550431 | 3:+42000979 | None:intergenic |
| AAAAGAGAGGTCAACTGGAG+TGG | 0.587592 | 3:-42000908 | MsG0380013621.01.T01:five_prime_UTR |
| AAAAGATGTATATTAAATAG+GGG | 0.558105 | 3:-42015954 | MsG0380013621.01.T01:five_prime_UTR |
| AAAAGCCTATTAATTTAAAT+AGG | 0.314207 | 3:+42004072 | None:intergenic |
| AAAAGCCTTACAAACCTATC+AGG | 0.403577 | 3:+42004113 | None:intergenic |
| AAAATAGTGTTACTCGAACC+TGG | 0.538064 | 3:-42003371 | MsG0380013621.01.T01:five_prime_UTR |
| AAAATTCGCCAAAATATCTA+AGG | 0.375878 | 3:-42013477 | MsG0380013621.01.T01:five_prime_UTR |
| AAACAACTCCGAGGCAGCGG+GGG | 0.530138 | 3:+42000948 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAAGGAGATAACTAGTTGA+TGG | - | Chr3:42017212-42017231 | MsG0380013621.01.T01:five_prime_UTR | 30.0% | |
| AAAGACCAAGGAAACTTTAA+TGG | - | Chr3:42006400-42006419 | MsG0380013621.01.T01:five_prime_UTR | 30.0% | |
| AAAGGAAAACTGTTTAGAAG+AGG | - | Chr3:41993004-41993023 | MsG0380013621.01.T01:intron | 30.0% | |
| AAAGGAGATAACTAGTTGAT+GGG | - | Chr3:42017213-42017232 | MsG0380013621.01.T01:five_prime_UTR | 30.0% | |
| AAATACTAAGCAACTCCTAA+TGG | - | Chr3:42009606-42009625 | MsG0380013621.01.T01:five_prime_UTR | 30.0% | |
| AACAAAAACAATCCGAACAT+GGG | + | Chr3:42009234-42009253 | None:intergenic | 30.0% | |
| AACAAAACCTCAATTGGTTA+AGG | - | Chr3:41994680-41994699 | MsG0380013621.01.T01:intron | 30.0% | |
| AACAATGCAAAGAAAGTTTG+TGG | - | Chr3:42017927-42017946 | MsG0380013621.01.T01:five_prime_UTR | 30.0% | |
| AACTGAATGTTTATGTCTAG+TGG | + | Chr3:42007724-42007743 | None:intergenic | 30.0% | |
| AAGAAACACTTATTTCCAAC+TGG | + | Chr3:41997448-41997467 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 41991746 | 42019665 | 41991746 | ID=MsG0380013621.01;Name=MsG0380013621.01 |
| Chr3 | mRNA | 41991746 | 42019665 | 41991746 | ID=MsG0380013621.01.T01;Parent=MsG0380013621.01;Name=MsG0380013621.01.T01;_AED=0.07;_eAED=0.07;_QI=23104|0|0.33|1|0|0|3|0|123 |
| Chr3 | exon | 41991746 | 41991880 | 41991746 | ID=MsG0380013621.01.T01:exon:2943;Parent=MsG0380013621.01.T01 |
| Chr3 | exon | 41995053 | 41995139 | 41995053 | ID=MsG0380013621.01.T01:exon:2942;Parent=MsG0380013621.01.T01 |
| Chr3 | exon | 41996412 | 42019665 | 41996412 | ID=MsG0380013621.01.T01:exon:2941;Parent=MsG0380013621.01.T01 |
| Chr3 | five_prime_UTR | 41996562 | 42019665 | 41996562 | ID=MsG0380013621.01.T01:five_prime_utr;Parent=MsG0380013621.01.T01 |
| Chr3 | CDS | 41996412 | 41996561 | 41996412 | ID=MsG0380013621.01.T01:cds;Parent=MsG0380013621.01.T01 |
| Chr3 | CDS | 41995053 | 41995139 | 41995053 | ID=MsG0380013621.01.T01:cds;Parent=MsG0380013621.01.T01 |
| Chr3 | CDS | 41991746 | 41991880 | 41991746 | ID=MsG0380013621.01.T01:cds;Parent=MsG0380013621.01.T01 |
| Gene Sequence |
>MsG0380013621.01.T01 ATGGTAGATATTGATTTTATGAAAATAAATAATATATCTTCACCCTGTAGGAACTTTCTTGGAGAAGAATTGGAGCAGTTGGATATCAATGAGCTTGAGCAAATAGAACTTCAGTTAGACTCTTCCTTGAAGACAATCAGGTCAAACAAGCATGTACTTATTGGTTTCCCATCTTCCTTTTTGATGGACACACAGGAAGAGATGCAACTAGAAACTAATAATATTCTGAGGAATAAGTTGGAGGAAATTAATGTGATCCTTCAACCAACATGGGAATCTAGTGAGCAAAATGCAACCTATAGCGGCCACCCTCACCAATCAGAGGGATACTTTGAGAAAGCATGTTGCAATAGTACATTGCAAATTGGGTAA |
| Protein sequence |
>MsG0380013621.01.T01 MVDIDFMKINNISSPCRNFLGEELEQLDINELEQIELQLDSSLKTIRSNKHVLIGFPSSFLMDTQEEMQLETNNILRNKLEEINVILQPTWESSEQNATYSGHPHQSEGYFEKACCNSTLQIG* |
