Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014120.01.T01 | XP_039688427.1 | 78.261 | 138 | 26 | 1 | 16 | 153 | 236 | 369 | 6.19E-65 | 213 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014120.01.T01 | A0A396IRE0 | 77.536 | 138 | 27 | 1 | 16 | 153 | 8 | 141 | 3.83e-65 | 212 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380014097.01 | MsG0380014120.01 | 0.822647 | 2.117641e-53 | 1.783171e-50 |
| MsG0380014103.01 | MsG0380014120.01 | 0.814137 | 1.795921e-51 | 1.195259e-48 |
| MsG0380014120.01 | MsG0780039170.01 | 0.801629 | 8.229084e-49 | 3.948759e-46 |
| MsG0380014120.01 | MsG0780039443.01 | 0.821986 | 3.014907e-53 | 2.491087e-50 |
| MsG0280007955.01 | MsG0380014120.01 | 0.829601 | 4.697901e-55 | 4.831275e-52 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014120.01.T01 | MTR_3g050500 | 68.841 | 138 | 39 | 1 | 16 | 153 | 400 | 533 | 3.39e-56 | 187 |
| MsG0380014120.01.T01 | MTR_3g051060 | 69.912 | 113 | 33 | 1 | 41 | 153 | 427 | 538 | 8.49e-48 | 165 |
| MsG0380014120.01.T01 | MTR_5g085340 | 43.697 | 119 | 61 | 2 | 39 | 153 | 434 | 550 | 5.97e-25 | 100 |
| MsG0380014120.01.T01 | MTR_3g058570 | 45.378 | 119 | 57 | 2 | 39 | 153 | 75 | 189 | 1.48e-23 | 95.5 |
| MsG0380014120.01.T01 | MTR_5g085350 | 46.364 | 110 | 55 | 2 | 46 | 153 | 422 | 529 | 1.83e-23 | 96.3 |
| MsG0380014120.01.T01 | MTR_5g085350 | 46.364 | 110 | 55 | 2 | 46 | 153 | 435 | 542 | 1.89e-23 | 96.3 |
| MsG0380014120.01.T01 | MTR_2g020510 | 47.170 | 106 | 51 | 2 | 49 | 154 | 391 | 491 | 4.79e-22 | 92.0 |
| MsG0380014120.01.T01 | MTR_2g020450 | 43.548 | 124 | 63 | 3 | 31 | 154 | 378 | 494 | 2.83e-21 | 90.1 |
| MsG0380014120.01.T01 | MTR_2g020470 | 36.290 | 124 | 52 | 3 | 31 | 154 | 377 | 473 | 3.47e-13 | 66.6 |
| MsG0380014120.01.T01 | MTR_5g085360 | 32.667 | 150 | 63 | 4 | 49 | 161 | 406 | 554 | 1.06e-11 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 28 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAATGAAAATGCGTTACTTT+TGG | 0.287460 | 3:-50089548 | MsG0380014120.01.T01:CDS |
| AGAGGGAATATTATATATTA+AGG | 0.298852 | 3:-50089749 | MsG0380014120.01.T01:CDS |
| GAATTATCTGCAAAGAAAAT+TGG | 0.332140 | 3:-50089676 | MsG0380014120.01.T01:CDS |
| AACACTGCTTGGAGGATATC+TGG | 0.365015 | 3:-50089382 | MsG0380014120.01.T01:CDS |
| TGACAACACCATTGTACATT+TGG | 0.385568 | 3:-50089431 | MsG0380014120.01.T01:CDS |
| CACAAGTTGTTGAGGGGCTA+TGG | 0.442530 | 3:-50089507 | MsG0380014120.01.T01:CDS |
| TTGTGGTTGTCTATTCTTCA+CGG | 0.443213 | 3:+50089525 | None:intergenic |
| TGAACCTCAAGATAAAATAG+AGG | 0.489446 | 3:-50089767 | MsG0380014120.01.T01:CDS |
| CATTGTTGGAATTAGTCTCA+TGG | 0.502983 | 3:+50089566 | None:intergenic |
| GATGTCCCACATCATTTGCA+TGG | 0.517564 | 3:+50089354 | None:intergenic |
| GACACAAAGAAAACACTGCT+TGG | 0.521447 | 3:-50089393 | MsG0380014120.01.T01:CDS |
| AAATGATGTGGGACATCAAA+TGG | 0.546182 | 3:-50089348 | MsG0380014120.01.T01:CDS |
| CATAGCCCCTCAACAACTTG+TGG | 0.552448 | 3:+50089508 | None:intergenic |
| GCTTAAATCTACAAGTGGAT+AGG | 0.560975 | 3:-50089456 | MsG0380014120.01.T01:CDS |
| TTGTAGATTTAAGCTCTCAA+AGG | 0.563349 | 3:+50089465 | None:intergenic |
| ACAAAGAAAACACTGCTTGG+AGG | 0.569158 | 3:-50089390 | MsG0380014120.01.T01:CDS |
| TATTTGATTGCAGCAAAACA+TGG | 0.571630 | 3:-50089637 | MsG0380014120.01.T01:CDS |
| AGACAACCACAAGTTGTTGA+GGG | 0.577599 | 3:-50089514 | MsG0380014120.01.T01:CDS |
| GAACCTCAAGATAAAATAGA+GGG | 0.590872 | 3:-50089766 | MsG0380014120.01.T01:CDS |
| TGCAAAGAAAATTGGCACAA+AGG | 0.593931 | 3:-50089668 | MsG0380014120.01.T01:CDS |
| TAGACAACCACAAGTTGTTG+AGG | 0.598311 | 3:-50089515 | MsG0380014120.01.T01:CDS |
| TGAGAGCTTAAATCTACAAG+TGG | 0.610439 | 3:-50089461 | MsG0380014120.01.T01:CDS |
| GTGCGGCCATGCAAATGATG+TGG | 0.618816 | 3:-50089360 | MsG0380014120.01.T01:CDS |
| GTGGGACATCAAATGGTACA+AGG | 0.651567 | 3:-50089341 | MsG0380014120.01.T01:CDS |
| TGCGGCCATGCAAATGATGT+GGG | 0.663238 | 3:-50089359 | MsG0380014120.01.T01:CDS |
| GACAACCACAAGTTGTTGAG+GGG | 0.680583 | 3:-50089513 | MsG0380014120.01.T01:CDS |
| TGCTTGGAGGATATCTGGTG+CGG | 0.701964 | 3:-50089377 | MsG0380014120.01.T01:CDS |
| AAATGCTGCCAAATGTACAA+TGG | 0.732344 | 3:+50089423 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATCAATTAAATTCACTAAT+AGG | + | Chr3:50089326-50089345 | None:intergenic | 15.0% |
| !! | AGAGGGAATATTATATATTA+AGG | - | Chr3:50089451-50089470 | MsG0380014120.01.T01:CDS | 20.0% |
| ! | GAATTATCTGCAAAGAAAAT+TGG | - | Chr3:50089524-50089543 | MsG0380014120.01.T01:CDS | 25.0% |
| GAACCTCAAGATAAAATAGA+GGG | - | Chr3:50089434-50089453 | MsG0380014120.01.T01:CDS | 30.0% | |
| TATTTGATTGCAGCAAAACA+TGG | - | Chr3:50089563-50089582 | MsG0380014120.01.T01:CDS | 30.0% | |
| TGAACCTCAAGATAAAATAG+AGG | - | Chr3:50089433-50089452 | MsG0380014120.01.T01:CDS | 30.0% | |
| TTGTAGATTTAAGCTCTCAA+AGG | + | Chr3:50089738-50089757 | None:intergenic | 30.0% | |
| ! | AAGTAACGCATTTTCATTGT+TGG | + | Chr3:50089651-50089670 | None:intergenic | 30.0% |
| ! | ATTCCCTCTATTTTATCTTG+AGG | + | Chr3:50089440-50089459 | None:intergenic | 30.0% |
| !! | CAATGAAAATGCGTTACTTT+TGG | - | Chr3:50089652-50089671 | MsG0380014120.01.T01:CDS | 30.0% |
| AAATGATGTGGGACATCAAA+TGG | - | Chr3:50089852-50089871 | MsG0380014120.01.T01:intron | 35.0% | |
| AAATGCTGCCAAATGTACAA+TGG | + | Chr3:50089780-50089799 | None:intergenic | 35.0% | |
| GCTTAAATCTACAAGTGGAT+AGG | - | Chr3:50089744-50089763 | MsG0380014120.01.T01:CDS | 35.0% | |
| TGACAACACCATTGTACATT+TGG | - | Chr3:50089769-50089788 | MsG0380014120.01.T01:CDS | 35.0% | |
| TGAGAGCTTAAATCTACAAG+TGG | - | Chr3:50089739-50089758 | MsG0380014120.01.T01:CDS | 35.0% | |
| TGCAAAGAAAATTGGCACAA+AGG | - | Chr3:50089532-50089551 | MsG0380014120.01.T01:CDS | 35.0% | |
| TTGTGGTTGTCTATTCTTCA+CGG | + | Chr3:50089678-50089697 | None:intergenic | 35.0% | |
| ! | CATTGTTGGAATTAGTCTCA+TGG | + | Chr3:50089637-50089656 | None:intergenic | 35.0% |
| ! | TAAGCTCTCAAAGGTTTTCA+TGG | + | Chr3:50089729-50089748 | None:intergenic | 35.0% |
| ACAAAGAAAACACTGCTTGG+AGG | - | Chr3:50089810-50089829 | MsG0380014120.01.T01:intron | 40.0% | |
| AGACAACCACAAGTTGTTGA+GGG | - | Chr3:50089686-50089705 | MsG0380014120.01.T01:CDS | 40.0% | |
| GACACAAAGAAAACACTGCT+TGG | - | Chr3:50089807-50089826 | MsG0380014120.01.T01:intron | 40.0% | |
| TAGACAACCACAAGTTGTTG+AGG | - | Chr3:50089685-50089704 | MsG0380014120.01.T01:CDS | 40.0% | |
| AACACTGCTTGGAGGATATC+TGG | - | Chr3:50089818-50089837 | MsG0380014120.01.T01:intron | 45.0% | |
| GACAACCACAAGTTGTTGAG+GGG | - | Chr3:50089687-50089706 | MsG0380014120.01.T01:CDS | 45.0% | |
| GATGTCCCACATCATTTGCA+TGG | + | Chr3:50089849-50089868 | None:intergenic | 45.0% | |
| GTGGGACATCAAATGGTACA+AGG | - | Chr3:50089859-50089878 | MsG0380014120.01.T01:intron | 45.0% | |
| CACAAGTTGTTGAGGGGCTA+TGG | - | Chr3:50089693-50089712 | MsG0380014120.01.T01:CDS | 50.0% | |
| CATAGCCCCTCAACAACTTG+TGG | + | Chr3:50089695-50089714 | None:intergenic | 50.0% | |
| TGCGGCCATGCAAATGATGT+GGG | - | Chr3:50089841-50089860 | MsG0380014120.01.T01:intron | 50.0% | |
| TGCTTGGAGGATATCTGGTG+CGG | - | Chr3:50089823-50089842 | MsG0380014120.01.T01:intron | 50.0% | |
| GTGCGGCCATGCAAATGATG+TGG | - | Chr3:50089840-50089859 | MsG0380014120.01.T01:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 50089300 | 50089922 | 50089300 | ID=MsG0380014120.01;Name=MsG0380014120.01 |
| Chr3 | mRNA | 50089300 | 50089922 | 50089300 | ID=MsG0380014120.01.T01;Parent=MsG0380014120.01;Name=MsG0380014120.01.T01;_AED=0.43;_eAED=0.43;_QI=0|0|0|0.5|0|0|2|0|166 |
| Chr3 | exon | 50089914 | 50089922 | 50089914 | ID=MsG0380014120.01.T01:exon:9181;Parent=MsG0380014120.01.T01 |
| Chr3 | exon | 50089300 | 50089791 | 50089300 | ID=MsG0380014120.01.T01:exon:9180;Parent=MsG0380014120.01.T01 |
| Chr3 | CDS | 50089914 | 50089922 | 50089914 | ID=MsG0380014120.01.T01:cds;Parent=MsG0380014120.01.T01 |
| Chr3 | CDS | 50089300 | 50089791 | 50089300 | ID=MsG0380014120.01.T01:cds;Parent=MsG0380014120.01.T01 |
| Gene Sequence |
| Protein sequence |
