Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014246.01.T01 | KEH41533.1 | 37.063 | 143 | 87 | 2 | 39 | 179 | 29 | 170 | 4.40E-23 | 105 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380014246.01 | MsG0780037146.01 | 0.803162 | 3.975986e-49 | 1.983950e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014246.01.T01 | MTR_1g052090 | 37.063 | 143 | 87 | 2 | 39 | 179 | 29 | 170 | 5.32e-27 | 105 |
| MsG0380014246.01.T01 | MTR_1g044605 | 34.807 | 181 | 103 | 5 | 1 | 179 | 1 | 168 | 2.83e-25 | 101 |
| MsG0380014246.01.T01 | MTR_3g455890 | 39.370 | 127 | 77 | 0 | 39 | 165 | 46 | 172 | 5.19e-25 | 100 |
| MsG0380014246.01.T01 | MTR_7g070630 | 32.796 | 186 | 105 | 4 | 2 | 184 | 8 | 176 | 1.89e-23 | 96.3 |
| MsG0380014246.01.T01 | MTR_0084s0060 | 45.098 | 102 | 56 | 0 | 39 | 140 | 46 | 147 | 5.91e-23 | 94.0 |
| MsG0380014246.01.T01 | MTR_3g080720 | 34.426 | 183 | 101 | 2 | 3 | 184 | 10 | 174 | 1.49e-20 | 88.2 |
| MsG0380014246.01.T01 | MTR_8g469580 | 34.559 | 136 | 85 | 3 | 39 | 171 | 44 | 178 | 1.54e-20 | 88.2 |
| MsG0380014246.01.T01 | MTR_5g079810 | 39.796 | 98 | 58 | 1 | 88 | 184 | 1 | 98 | 1.12e-17 | 79.3 |
| MsG0380014246.01.T01 | MTR_0126s0010 | 51.724 | 58 | 28 | 0 | 127 | 184 | 18 | 75 | 1.38e-14 | 70.5 |
| MsG0380014246.01.T01 | MTR_5g039510 | 34.444 | 90 | 56 | 2 | 80 | 169 | 40 | 126 | 4.05e-13 | 67.0 |
| MsG0380014246.01.T01 | MTR_8g056180 | 36.667 | 90 | 54 | 2 | 88 | 175 | 1 | 89 | 1.75e-11 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 57 sgRNAs with CRISPR-Local
Find 69 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTTGAGCGAGTGTTTCTT+TGG | 0.305202 | 3:-51840004 | None:intergenic |
| ATGAAGAGTTTCGAAGTAAT+AGG | 0.315020 | 3:+51840143 | MsG0380014246.01.T01:CDS |
| GATTTGCTTGTGGATGGCTT+GGG | 0.319003 | 3:-51840038 | None:intergenic |
| CGAAACTCTTCATAGATTGA+TGG | 0.338129 | 3:-51840133 | None:intergenic |
| GGATTTGCTTGTGGATGGCT+TGG | 0.341662 | 3:-51840039 | None:intergenic |
| AGCCATTCATTAAGCGCTTC+AGG | 0.349684 | 3:-51840365 | None:intergenic |
| TGAAGAGTTTCGAAGTAATA+GGG | 0.363581 | 3:+51840144 | MsG0380014246.01.T01:CDS |
| TGTGGATGGCTTGGGGTTGG+TGG | 0.363953 | 3:-51840030 | None:intergenic |
| CTTAATTCAGATTCAAAGAA+AGG | 0.368741 | 3:-51840203 | None:intergenic |
| TCATAGATTGATGGAACTCT+TGG | 0.374882 | 3:-51840124 | None:intergenic |
| GGATGCGGCACGAGGACGTT+TGG | 0.402925 | 3:-51840060 | None:intergenic |
| GCTTGTGGATGGCTTGGGGT+TGG | 0.407578 | 3:-51840033 | None:intergenic |
| TGAGCGAGTGTTTCTTTGGT+TGG | 0.423762 | 3:-51840000 | None:intergenic |
| GGGCTTGGTCATTGATGAAA+TGG | 0.433108 | 3:-51840103 | None:intergenic |
| TGTGTAGGCCTGGGTCTACT+TGG | 0.455071 | 3:+51840483 | MsG0380014246.01.T01:CDS |
| GCCATTCATTAAGCGCTTCA+GGG | 0.464356 | 3:-51840364 | None:intergenic |
| ATTGATGGAACTCTTGGGCT+TGG | 0.464533 | 3:-51840118 | None:intergenic |
| CATAGATTGATGGAACTCTT+GGG | 0.467923 | 3:-51840123 | None:intergenic |
| TCAACCACCGGAGGAGTGTT+GGG | 0.475896 | 3:+51840394 | MsG0380014246.01.T01:CDS |
| ATTACCACGATGTGTAGGCC+TGG | 0.485912 | 3:+51840473 | MsG0380014246.01.T01:CDS |
| TCTTTGAATCTGAATTAAGA+AGG | 0.491499 | 3:+51840207 | MsG0380014246.01.T01:CDS |
| TCTCAGCATAGGTAAGCATC+CGG | 0.494255 | 3:-51840514 | None:intergenic |
| TTCAACCACCGGAGGAGTGT+TGG | 0.497684 | 3:+51840393 | MsG0380014246.01.T01:CDS |
| TTCTCAACCCAACACTCCTC+CGG | 0.500312 | 3:-51840401 | None:intergenic |
| CGTTTGGATTTGCTTGTGGA+TGG | 0.502038 | 3:-51840044 | None:intergenic |
| TCGAATTCTTTGCAAATGCA+AGG | 0.510244 | 3:+51840288 | MsG0380014246.01.T01:CDS |
| AAACTGAGGAGGGATAGATT+TGG | 0.512322 | 3:+51840422 | MsG0380014246.01.T01:CDS |
| CGCTTCAGGGCTGAAATCAA+TGG | 0.516932 | 3:-51840351 | None:intergenic |
| TTACCACGATGTGTAGGCCT+GGG | 0.518048 | 3:+51840474 | MsG0380014246.01.T01:CDS |
| CGAATTCTTTGCAAATGCAA+GGG | 0.522458 | 3:+51840289 | MsG0380014246.01.T01:CDS |
| GTTTCGAAGTAATAGGGGAG+AGG | 0.527550 | 3:+51840150 | MsG0380014246.01.T01:CDS |
| ACCCCGAACAAAAGATTGAA+AGG | 0.531437 | 3:-51840324 | None:intergenic |
| GCGAGTGTTTCTTTGGTTGG+AGG | 0.533251 | 3:-51839997 | None:intergenic |
| GCCCTGAAGCGCTTAATGAA+TGG | 0.536762 | 3:+51840363 | MsG0380014246.01.T01:CDS |
| ATTTGCTTGTGGATGGCTTG+GGG | 0.543117 | 3:-51840037 | None:intergenic |
| AGGACGTTTGGATTTGCTTG+TGG | 0.548218 | 3:-51840048 | None:intergenic |
| TGAATCTGAATTAAGAAGGA+GGG | 0.553477 | 3:+51840211 | MsG0380014246.01.T01:CDS |
| AGTGTTGGGTTGAGAAACTG+AGG | 0.553972 | 3:+51840408 | MsG0380014246.01.T01:CDS |
| AAAAGATTGAAAGGGTGTAT+CGG | 0.557262 | 3:-51840315 | None:intergenic |
| CCCCGAACAAAAGATTGAAA+GGG | 0.561185 | 3:-51840323 | None:intergenic |
| GAATCTGAATTAAGAAGGAG+GGG | 0.571010 | 3:+51840212 | MsG0380014246.01.T01:CDS |
| TCAACCCAACACTCCTCCGG+TGG | 0.575000 | 3:-51840398 | None:intergenic |
| TCATGGTAACATCTCAGCAT+AGG | 0.590203 | 3:-51840525 | None:intergenic |
| TTGAATCTGAATTAAGAAGG+AGG | 0.590475 | 3:+51840210 | MsG0380014246.01.T01:CDS |
| CGACGAAGCAAACAACAAAA+CGG | 0.592685 | 3:+51840259 | MsG0380014246.01.T01:CDS |
| GGATAGATTTGGTATGACAG+AGG | 0.604363 | 3:+51840433 | MsG0380014246.01.T01:CDS |
| GTTGGGTTGAGAAACTGAGG+AGG | 0.607238 | 3:+51840411 | MsG0380014246.01.T01:CDS |
| ATGGCTGAATGTTCAACCAC+CGG | 0.613044 | 3:+51840382 | MsG0380014246.01.T01:CDS |
| TTGGGTTGAGAAACTGAGGA+GGG | 0.613278 | 3:+51840412 | MsG0380014246.01.T01:CDS |
| CTCAGCATAGGTAAGCATCC+GGG | 0.614157 | 3:-51840513 | None:intergenic |
| GAAGAGTTTCGAAGTAATAG+GGG | 0.617876 | 3:+51840145 | MsG0380014246.01.T01:CDS |
| GGTGGTTCGGATGCGGCACG+AGG | 0.626471 | 3:-51840068 | None:intergenic |
| ACATCATTACCACGATGTGT+AGG | 0.634062 | 3:+51840468 | MsG0380014246.01.T01:CDS |
| AATCTGAATTAAGAAGGAGG+GGG | 0.642060 | 3:+51840213 | MsG0380014246.01.T01:CDS |
| TTGGTCATTGATGAAATGGA+TGG | 0.651905 | 3:-51840099 | None:intergenic |
| GCTGAATGTTCAACCACCGG+AGG | 0.709126 | 3:+51840385 | MsG0380014246.01.T01:CDS |
| AGACCCAGGCCTACACATCG+TGG | 0.713382 | 3:-51840477 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | CTTAATTCAGATTCAAAGAA+AGG | - | Chr3:51840206-51840225 | None:intergenic | 25.0% |
| ! | TCTTTGAATCTGAATTAAGA+AGG | + | Chr3:51840207-51840226 | MsG0380014246.01.T01:CDS | 25.0% |
| ATGAAGAGTTTCGAAGTAAT+AGG | + | Chr3:51840143-51840162 | MsG0380014246.01.T01:CDS | 30.0% | |
| TGAAGAGTTTCGAAGTAATA+GGG | + | Chr3:51840144-51840163 | MsG0380014246.01.T01:CDS | 30.0% | |
| TGAATCTGAATTAAGAAGGA+GGG | + | Chr3:51840211-51840230 | MsG0380014246.01.T01:CDS | 30.0% | |
| TTGAATCTGAATTAAGAAGG+AGG | + | Chr3:51840210-51840229 | MsG0380014246.01.T01:CDS | 30.0% | |
| ! | AAAAGATTGAAAGGGTGTAT+CGG | - | Chr3:51840318-51840337 | None:intergenic | 30.0% |
| AATCTGAATTAAGAAGGAGG+GGG | + | Chr3:51840213-51840232 | MsG0380014246.01.T01:CDS | 35.0% | |
| CATAGATTGATGGAACTCTT+GGG | - | Chr3:51840126-51840145 | None:intergenic | 35.0% | |
| CGAAACTCTTCATAGATTGA+TGG | - | Chr3:51840136-51840155 | None:intergenic | 35.0% | |
| CGAATTCTTTGCAAATGCAA+GGG | + | Chr3:51840289-51840308 | MsG0380014246.01.T01:CDS | 35.0% | |
| GAAGAGTTTCGAAGTAATAG+GGG | + | Chr3:51840145-51840164 | MsG0380014246.01.T01:CDS | 35.0% | |
| GAATCTGAATTAAGAAGGAG+GGG | + | Chr3:51840212-51840231 | MsG0380014246.01.T01:CDS | 35.0% | |
| TCATAGATTGATGGAACTCT+TGG | - | Chr3:51840127-51840146 | None:intergenic | 35.0% | |
| TCGAATTCTTTGCAAATGCA+AGG | + | Chr3:51840288-51840307 | MsG0380014246.01.T01:CDS | 35.0% | |
| TTGGTCATTGATGAAATGGA+TGG | - | Chr3:51840102-51840121 | None:intergenic | 35.0% | |
| ! | ACCCTTTCAATCTTTTGTTC+GGG | + | Chr3:51840322-51840341 | MsG0380014246.01.T01:CDS | 35.0% |
| ! | CACCCTTTCAATCTTTTGTT+CGG | + | Chr3:51840321-51840340 | MsG0380014246.01.T01:CDS | 35.0% |
| AAACTGAGGAGGGATAGATT+TGG | + | Chr3:51840422-51840441 | MsG0380014246.01.T01:CDS | 40.0% | |
| ACATCATTACCACGATGTGT+AGG | + | Chr3:51840468-51840487 | MsG0380014246.01.T01:CDS | 40.0% | |
| ACCCCGAACAAAAGATTGAA+AGG | - | Chr3:51840327-51840346 | None:intergenic | 40.0% | |
| CCCCGAACAAAAGATTGAAA+GGG | - | Chr3:51840326-51840345 | None:intergenic | 40.0% | |
| CGACGAAGCAAACAACAAAA+CGG | + | Chr3:51840259-51840278 | MsG0380014246.01.T01:CDS | 40.0% | |
| TCATGGTAACATCTCAGCAT+AGG | - | Chr3:51840528-51840547 | None:intergenic | 40.0% | |
| TCTTTGAGCGAGTGTTTCTT+TGG | - | Chr3:51840007-51840026 | None:intergenic | 40.0% | |
| ! | ATGAAATGGATGGCGTTTTC+GGG | - | Chr3:51840092-51840111 | None:intergenic | 40.0% |
| ! | CCCTTTCAATCTTTTGTTCG+GGG | + | Chr3:51840323-51840342 | MsG0380014246.01.T01:CDS | 40.0% |
| ! | GATGAAATGGATGGCGTTTT+CGG | - | Chr3:51840093-51840112 | None:intergenic | 40.0% |
| ! | GGATAGATTTGGTATGACAG+AGG | + | Chr3:51840433-51840452 | MsG0380014246.01.T01:CDS | 40.0% |
| AGCCATTCATTAAGCGCTTC+AGG | - | Chr3:51840368-51840387 | None:intergenic | 45.0% | |
| ATGGCTGAATGTTCAACCAC+CGG | + | Chr3:51840382-51840401 | MsG0380014246.01.T01:CDS | 45.0% | |
| ATTGATGGAACTCTTGGGCT+TGG | - | Chr3:51840121-51840140 | None:intergenic | 45.0% | |
| ATTTGCTTGTGGATGGCTTG+GGG | - | Chr3:51840040-51840059 | None:intergenic | 45.0% | |
| GCCATTCATTAAGCGCTTCA+GGG | - | Chr3:51840367-51840386 | None:intergenic | 45.0% | |
| GGTCTACTTGGAAAAAGTCC+CGG | + | Chr3:51840495-51840514 | MsG0380014246.01.T01:CDS | 45.0% | |
| GTTTCGAAGTAATAGGGGAG+AGG | + | Chr3:51840150-51840169 | MsG0380014246.01.T01:CDS | 45.0% | |
| TCTCAGCATAGGTAAGCATC+CGG | - | Chr3:51840517-51840536 | None:intergenic | 45.0% | |
| TGAGCGAGTGTTTCTTTGGT+TGG | - | Chr3:51840003-51840022 | None:intergenic | 45.0% | |
| ! | AGGACGTTTGGATTTGCTTG+TGG | - | Chr3:51840051-51840070 | None:intergenic | 45.0% |
| ! | CGTTTGGATTTGCTTGTGGA+TGG | - | Chr3:51840047-51840066 | None:intergenic | 45.0% |
| ! | GATTTGCTTGTGGATGGCTT+GGG | - | Chr3:51840041-51840060 | None:intergenic | 45.0% |
| ! | GGGCTTGGTCATTGATGAAA+TGG | - | Chr3:51840106-51840125 | None:intergenic | 45.0% |
| !! | AGTGTTGGGTTGAGAAACTG+AGG | + | Chr3:51840408-51840427 | MsG0380014246.01.T01:CDS | 45.0% |
| !! | GACTTTTTCCAAGTAGACCC+AGG | - | Chr3:51840494-51840513 | None:intergenic | 45.0% |
| !! | TTGGGTTGAGAAACTGAGGA+GGG | + | Chr3:51840412-51840431 | MsG0380014246.01.T01:CDS | 45.0% |
| ATTACCACGATGTGTAGGCC+TGG | + | Chr3:51840473-51840492 | MsG0380014246.01.T01:CDS | 50.0% | |
| CGCTTCAGGGCTGAAATCAA+TGG | - | Chr3:51840354-51840373 | None:intergenic | 50.0% | |
| CTCAGCATAGGTAAGCATCC+GGG | - | Chr3:51840516-51840535 | None:intergenic | 50.0% | |
| CTGAATTAAGAAGGAGGGGG+TGG | + | Chr3:51840216-51840235 | MsG0380014246.01.T01:CDS | 50.0% | |
| GCGAGTGTTTCTTTGGTTGG+AGG | - | Chr3:51840000-51840019 | None:intergenic | 50.0% | |
| TTACCACGATGTGTAGGCCT+GGG | + | Chr3:51840474-51840493 | MsG0380014246.01.T01:CDS | 50.0% | |
| TTCTCAACCCAACACTCCTC+CGG | - | Chr3:51840404-51840423 | None:intergenic | 50.0% | |
| ! | GCCCTGAAGCGCTTAATGAA+TGG | + | Chr3:51840363-51840382 | MsG0380014246.01.T01:CDS | 50.0% |
| ! | GGATTTGCTTGTGGATGGCT+TGG | - | Chr3:51840042-51840061 | None:intergenic | 50.0% |
| !! | AAATGGATGGCGTTTTCGGG+TGG | - | Chr3:51840089-51840108 | None:intergenic | 50.0% |
| !! | GTTGGGTTGAGAAACTGAGG+AGG | + | Chr3:51840411-51840430 | MsG0380014246.01.T01:CDS | 50.0% |
| GCTGAATGTTCAACCACCGG+AGG | + | Chr3:51840385-51840404 | MsG0380014246.01.T01:CDS | 55.0% | |
| TAAGAAGGAGGGGGTGGATG+AGG | + | Chr3:51840222-51840241 | MsG0380014246.01.T01:CDS | 55.0% | |
| TGTGTAGGCCTGGGTCTACT+TGG | + | Chr3:51840483-51840502 | MsG0380014246.01.T01:CDS | 55.0% | |
| ! | TTCAACCACCGGAGGAGTGT+TGG | + | Chr3:51840393-51840412 | MsG0380014246.01.T01:CDS | 55.0% |
| !! | TCAACCACCGGAGGAGTGTT+GGG | + | Chr3:51840394-51840413 | MsG0380014246.01.T01:CDS | 55.0% |
| !!! | GATGGCGTTTTCGGGTGGTT+CGG | - | Chr3:51840084-51840103 | None:intergenic | 55.0% |
| !!! | GTTTTCGGGTGGTTCGGATG+CGG | - | Chr3:51840078-51840097 | None:intergenic | 55.0% |
| AGACCCAGGCCTACACATCG+TGG | - | Chr3:51840480-51840499 | None:intergenic | 60.0% | |
| TCAACCCAACACTCCTCCGG+TGG | - | Chr3:51840401-51840420 | None:intergenic | 60.0% | |
| ! | GCTTGTGGATGGCTTGGGGT+TGG | - | Chr3:51840036-51840055 | None:intergenic | 60.0% |
| ! | TGTGGATGGCTTGGGGTTGG+TGG | - | Chr3:51840033-51840052 | None:intergenic | 60.0% |
| GGATGCGGCACGAGGACGTT+TGG | - | Chr3:51840063-51840082 | None:intergenic | 65.0% | |
| ! | GGTGGTTCGGATGCGGCACG+AGG | - | Chr3:51840071-51840090 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 51839993 | 51840547 | 51839993 | ID=MsG0380014246.01;Name=MsG0380014246.01 |
| Chr3 | mRNA | 51839993 | 51840547 | 51839993 | ID=MsG0380014246.01.T01;Parent=MsG0380014246.01;Name=MsG0380014246.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|184 |
| Chr3 | exon | 51839993 | 51840547 | 51839993 | ID=MsG0380014246.01.T01:exon:10128;Parent=MsG0380014246.01.T01 |
| Chr3 | CDS | 51839993 | 51840547 | 51839993 | ID=MsG0380014246.01.T01:cds;Parent=MsG0380014246.01.T01 |
| Gene Sequence |
| Protein sequence |
