Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014346.01.T01 | XP_039686465.1 | 92.179 | 179 | 14 | 0 | 1 | 179 | 1 | 179 | 5.85E-118 | 346 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014346.01.T01 | A0A6A5MKU4 | 60.452 | 177 | 66 | 1 | 3 | 179 | 6 | 178 | 2.04e-64 | 211 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280007926.01 | MsG0380014346.01 | 0.807101 | 5.957334e-50 | 3.289053e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014346.01.T01 | MTR_8g055900 | 90.816 | 98 | 9 | 0 | 1 | 98 | 1 | 98 | 1.54e-58 | 179 |
| MsG0380014346.01.T01 | MTR_7g056633 | 75.000 | 96 | 24 | 0 | 84 | 179 | 12 | 107 | 9.79e-45 | 145 |
| MsG0380014346.01.T01 | MTR_8g463880 | 57.609 | 92 | 39 | 0 | 88 | 179 | 48 | 139 | 1.02e-31 | 112 |
| MsG0380014346.01.T01 | MTR_5g063300 | 48.780 | 82 | 42 | 0 | 98 | 179 | 44 | 125 | 1.92e-19 | 80.9 |
| MsG0380014346.01.T01 | MTR_7g094990 | 40.426 | 94 | 54 | 1 | 88 | 179 | 55 | 148 | 7.02e-14 | 69.3 |
| MsG0380014346.01.T01 | MTR_4g117940 | 47.368 | 76 | 40 | 0 | 104 | 179 | 75 | 150 | 2.39e-13 | 65.5 |
| MsG0380014346.01.T01 | MTR_4g055725 | 40.789 | 76 | 45 | 0 | 104 | 179 | 78 | 153 | 9.36e-11 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014346.01.T01 | AT5G41980 | 39.241 | 79 | 48 | 0 | 101 | 179 | 39 | 117 | 3.37e-12 | 64.7 |
Find 22 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAACGTCTACGCTCACTTTG+TGG | 0.360598 | 3:-53255603 | None:intergenic |
| GCAAAATTCTTGCATATTGT+AGG | 0.403388 | 3:+53255771 | MsG0380014346.01.T01:CDS |
| CACAACTGCATGAATGCTTC+AGG | 0.434104 | 3:-53255684 | None:intergenic |
| CATGTTACAAGGCATAATTA+TGG | 0.441824 | 3:-53255880 | None:intergenic |
| AAAATACTTGCCATGTTACA+AGG | 0.470518 | 3:-53255891 | None:intergenic |
| ACGCTCACTTTGTGGAGAAT+AGG | 0.478427 | 3:-53255595 | None:intergenic |
| TGTCGGGACATCATACGAAT+GGG | 0.483003 | 3:+53255660 | MsG0380014346.01.T01:CDS |
| GAAGATGAAATGATTGATGT+TGG | 0.483282 | 3:+53255426 | MsG0380014346.01.T01:CDS |
| ATGGAAAGGAGGCAACATAT+TGG | 0.496501 | 3:+53255492 | MsG0380014346.01.T01:CDS |
| TTTGATTCAAATACAATGCA+TGG | 0.500520 | 3:-53255542 | None:intergenic |
| AATAATAGATGAAGTGGACT+TGG | 0.510368 | 3:+53255401 | MsG0380014346.01.T01:CDS |
| GTGTCTAAAACTAAGAGGTA+CGG | 0.521806 | 3:+53255704 | MsG0380014346.01.T01:CDS |
| CATAATTATGCCTTGTAACA+TGG | 0.523134 | 3:+53255881 | MsG0380014346.01.T01:CDS |
| TGTCTAAAACTAAGAGGTAC+GGG | 0.538246 | 3:+53255705 | MsG0380014346.01.T01:CDS |
| AATTGAAATAATAGATGAAG+TGG | 0.540512 | 3:+53255395 | MsG0380014346.01.T01:CDS |
| AAGAGGTACGGGTATAGTAA+AGG | 0.555417 | 3:+53255716 | MsG0380014346.01.T01:CDS |
| CAGTTGTGTCTAAAACTAAG+AGG | 0.556135 | 3:+53255699 | MsG0380014346.01.T01:CDS |
| GTGTCGGGACATCATACGAA+TGG | 0.561288 | 3:+53255659 | MsG0380014346.01.T01:CDS |
| GTCTATTGTAGACGAGCAAG+TGG | 0.572123 | 3:+53255749 | MsG0380014346.01.T01:CDS |
| AGTGCACACAGATAAGTGTC+GGG | 0.599113 | 3:+53255644 | MsG0380014346.01.T01:CDS |
| TAGTGCACACAGATAAGTGT+CGG | 0.605090 | 3:+53255643 | MsG0380014346.01.T01:CDS |
| GTCGGGACATCATACGAATG+GGG | 0.688822 | 3:+53255661 | MsG0380014346.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTGAAATAATAGATGAAG+TGG | + | Chr3:53255395-53255414 | MsG0380014346.01.T01:CDS | 20.0% |
| ! | AAATCATGCTAAAAAAGAGA+TGG | + | Chr3:53255473-53255492 | MsG0380014346.01.T01:CDS | 25.0% |
| ! | TTTGATTCAAATACAATGCA+TGG | - | Chr3:53255545-53255564 | None:intergenic | 25.0% |
| AAAATACTTGCCATGTTACA+AGG | - | Chr3:53255894-53255913 | None:intergenic | 30.0% | |
| ATGCTAAAAAAGAGATGGAA+AGG | + | Chr3:53255478-53255497 | MsG0380014346.01.T01:CDS | 30.0% | |
| CATAATTATGCCTTGTAACA+TGG | + | Chr3:53255881-53255900 | MsG0380014346.01.T01:CDS | 30.0% | |
| CATGTTACAAGGCATAATTA+TGG | - | Chr3:53255883-53255902 | None:intergenic | 30.0% | |
| GAAGATGAAATGATTGATGT+TGG | + | Chr3:53255426-53255445 | MsG0380014346.01.T01:CDS | 30.0% | |
| GCAAAATTCTTGCATATTGT+AGG | + | Chr3:53255771-53255790 | MsG0380014346.01.T01:CDS | 30.0% | |
| ! | AATAATAGATGAAGTGGACT+TGG | + | Chr3:53255401-53255420 | MsG0380014346.01.T01:CDS | 30.0% |
| !!! | AGTATTTTGAAACATGCAGT+AGG | + | Chr3:53255906-53255925 | MsG0380014346.01.T01:CDS | 30.0% |
| !!! | GTATTTTGAAACATGCAGTA+GGG | + | Chr3:53255907-53255926 | MsG0380014346.01.T01:CDS | 30.0% |
| CTAAAAAAGAGATGGAAAGG+AGG | + | Chr3:53255481-53255500 | MsG0380014346.01.T01:CDS | 35.0% | |
| ! | CAGTTGTGTCTAAAACTAAG+AGG | + | Chr3:53255699-53255718 | MsG0380014346.01.T01:CDS | 35.0% |
| ! | GTGTCTAAAACTAAGAGGTA+CGG | + | Chr3:53255704-53255723 | MsG0380014346.01.T01:CDS | 35.0% |
| ! | TGTCTAAAACTAAGAGGTAC+GGG | + | Chr3:53255705-53255724 | MsG0380014346.01.T01:CDS | 35.0% |
| ATGGAAAGGAGGCAACATAT+TGG | + | Chr3:53255492-53255511 | MsG0380014346.01.T01:CDS | 40.0% | |
| TAGTGCACACAGATAAGTGT+CGG | + | Chr3:53255643-53255662 | MsG0380014346.01.T01:CDS | 40.0% | |
| ! | AAGAGGTACGGGTATAGTAA+AGG | + | Chr3:53255716-53255735 | MsG0380014346.01.T01:CDS | 40.0% |
| ACGCTCACTTTGTGGAGAAT+AGG | - | Chr3:53255598-53255617 | None:intergenic | 45.0% | |
| AGTGCACACAGATAAGTGTC+GGG | + | Chr3:53255644-53255663 | MsG0380014346.01.T01:CDS | 45.0% | |
| CACAACTGCATGAATGCTTC+AGG | - | Chr3:53255687-53255706 | None:intergenic | 45.0% | |
| GTCTATTGTAGACGAGCAAG+TGG | + | Chr3:53255749-53255768 | MsG0380014346.01.T01:CDS | 45.0% | |
| TGTCGGGACATCATACGAAT+GGG | + | Chr3:53255660-53255679 | MsG0380014346.01.T01:CDS | 45.0% | |
| CAACGTCTACGCTCACTTTG+TGG | - | Chr3:53255606-53255625 | None:intergenic | 50.0% | |
| GTCGGGACATCATACGAATG+GGG | + | Chr3:53255661-53255680 | MsG0380014346.01.T01:CDS | 50.0% | |
| GTGTCGGGACATCATACGAA+TGG | + | Chr3:53255659-53255678 | MsG0380014346.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 53255351 | 53255938 | 53255351 | ID=MsG0380014346.01;Name=MsG0380014346.01 |
| Chr3 | mRNA | 53255351 | 53255938 | 53255351 | ID=MsG0380014346.01.T01;Parent=MsG0380014346.01;Name=MsG0380014346.01.T01;_AED=0.41;_eAED=0.41;_QI=0|-1|0|1|-1|1|1|0|195 |
| Chr3 | exon | 53255351 | 53255938 | 53255351 | ID=MsG0380014346.01.T01:exon:10581;Parent=MsG0380014346.01.T01 |
| Chr3 | CDS | 53255351 | 53255938 | 53255351 | ID=MsG0380014346.01.T01:cds;Parent=MsG0380014346.01.T01 |
| Gene Sequence |
| Protein sequence |
