Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007926.01.T01 | XP_039686465.1 | 93.125 | 160 | 11 | 0 | 1 | 160 | 1 | 160 | 1.61E-104 | 311 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007926.01.T01 | G7LA58 | 90.816 | 98 | 9 | 0 | 1 | 98 | 1 | 98 | 2.26e-55 | 180 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180001173.01 | MsG0280007926.01 | 0.803686 | 3.096880e-49 | 1.566169e-46 |
| MsG0180005443.01 | MsG0280007926.01 | 0.813493 | 2.488829e-51 | 1.627851e-48 |
| MsG0180005451.01 | MsG0280007926.01 | 0.817302 | 3.537854e-52 | 2.566839e-49 |
| MsG0280006367.01 | MsG0280007926.01 | 0.800903 | 1.158519e-48 | 5.458684e-46 |
| MsG0280007926.01 | MsG0380014346.01 | 0.807101 | 5.957334e-50 | 3.289053e-47 |
| MsG0280007926.01 | MsG0480022228.01 | 0.820174 | 7.886331e-53 | 6.194942e-50 |
| MsG0280007926.01 | MsG0780041390.01 | 0.805250 | 1.461961e-49 | 7.696867e-47 |
| MsG0280007926.01 | MsG0880042760.01 | 0.802795 | 4.735775e-49 | 2.341099e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007926.01.T01 | MTR_8g055900 | 90.816 | 98 | 9 | 0 | 1 | 98 | 1 | 98 | 5.73e-59 | 180 |
| MsG0280007926.01.T01 | MTR_7g056633 | 72.727 | 77 | 21 | 0 | 84 | 160 | 12 | 88 | 4.77e-34 | 117 |
| MsG0280007926.01.T01 | MTR_8g463880 | 54.795 | 73 | 33 | 0 | 88 | 160 | 48 | 120 | 2.87e-23 | 90.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 23 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTACAATATGCAAGAATTT+TGG | 0.207961 | 2:-23073514 | None:intergenic |
| TATCTTTCTTCTTTATCGTT+CGG | 0.291860 | 2:+23073560 | MsG0280007926.01.T01:CDS |
| ATCTTTCTTCTTTATCGTTC+GGG | 0.300279 | 2:+23073561 | MsG0280007926.01.T01:CDS |
| CAACGTCTACGCTCACTTTG+TGG | 0.360598 | 2:-23073346 | None:intergenic |
| CCAAAATTCTTGCATATTGT+AGG | 0.369864 | 2:+23073514 | MsG0280007926.01.T01:CDS |
| CACAACTGCATGAATGCTTC+AGG | 0.434104 | 2:-23073427 | None:intergenic |
| ACGCTCACTTTGTGGAGAAT+AGG | 0.478427 | 2:-23073338 | None:intergenic |
| TGTCGGGACATCATACGAAT+GGG | 0.483003 | 2:+23073403 | MsG0280007926.01.T01:CDS |
| GAAGATGAAATGATTGATGT+TGG | 0.483282 | 2:+23073169 | MsG0280007926.01.T01:CDS |
| ATGGAAAGGAGGCAACATAT+TGG | 0.496501 | 2:+23073235 | MsG0280007926.01.T01:CDS |
| TTTGATTCAAATACAATGCA+TGG | 0.500520 | 2:-23073285 | None:intergenic |
| AATAATAGATGAAGTGGACT+TGG | 0.510368 | 2:+23073144 | MsG0280007926.01.T01:CDS |
| GTGTCTAAAACTAAGAGGTA+CGG | 0.521806 | 2:+23073447 | MsG0280007926.01.T01:CDS |
| TGTCTAAAACTAAGAGGTAC+GGG | 0.538246 | 2:+23073448 | MsG0280007926.01.T01:CDS |
| AATTGAAATAATAGATGAAG+TGG | 0.540512 | 2:+23073138 | MsG0280007926.01.T01:CDS |
| CAGTTGTGTCTAAAACTAAG+AGG | 0.556135 | 2:+23073442 | MsG0280007926.01.T01:CDS |
| GTGTCGGGACATCATACGAA+TGG | 0.561288 | 2:+23073402 | MsG0280007926.01.T01:CDS |
| AGTGCACACAGATAAGTGTC+GGG | 0.599113 | 2:+23073387 | MsG0280007926.01.T01:CDS |
| TAGTGCACACAGATAAGTGT+CGG | 0.605090 | 2:+23073386 | MsG0280007926.01.T01:CDS |
| GTCGGGACATCATACGAATG+GGG | 0.688822 | 2:+23073404 | MsG0280007926.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTGAAATAATAGATGAAG+TGG | + | Chr2:23073138-23073157 | MsG0280007926.01.T01:CDS | 20.0% |
| ! | AAATCATGCTAAAAAAGAGA+TGG | + | Chr2:23073216-23073235 | MsG0280007926.01.T01:CDS | 25.0% |
| ! | TATCTTTCTTCTTTATCGTT+CGG | + | Chr2:23073560-23073579 | MsG0280007926.01.T01:CDS | 25.0% |
| ! | TTTGATTCAAATACAATGCA+TGG | - | Chr2:23073288-23073307 | None:intergenic | 25.0% |
| ATCTTTCTTCTTTATCGTTC+GGG | + | Chr2:23073561-23073580 | MsG0280007926.01.T01:CDS | 30.0% | |
| ATGCTAAAAAAGAGATGGAA+AGG | + | Chr2:23073221-23073240 | MsG0280007926.01.T01:CDS | 30.0% | |
| CCAAAATTCTTGCATATTGT+AGG | + | Chr2:23073514-23073533 | MsG0280007926.01.T01:CDS | 30.0% | |
| GAAGATGAAATGATTGATGT+TGG | + | Chr2:23073169-23073188 | MsG0280007926.01.T01:CDS | 30.0% | |
| ! | AATAATAGATGAAGTGGACT+TGG | + | Chr2:23073144-23073163 | MsG0280007926.01.T01:CDS | 30.0% |
| ! | CCTACAATATGCAAGAATTT+TGG | - | Chr2:23073517-23073536 | None:intergenic | 30.0% |
| CTAAAAAAGAGATGGAAAGG+AGG | + | Chr2:23073224-23073243 | MsG0280007926.01.T01:CDS | 35.0% | |
| ! | CAGTTGTGTCTAAAACTAAG+AGG | + | Chr2:23073442-23073461 | MsG0280007926.01.T01:CDS | 35.0% |
| ! | GTGTCTAAAACTAAGAGGTA+CGG | + | Chr2:23073447-23073466 | MsG0280007926.01.T01:CDS | 35.0% |
| ! | TGTCTAAAACTAAGAGGTAC+GGG | + | Chr2:23073448-23073467 | MsG0280007926.01.T01:CDS | 35.0% |
| ATGGAAAGGAGGCAACATAT+TGG | + | Chr2:23073235-23073254 | MsG0280007926.01.T01:CDS | 40.0% | |
| TAGTGCACACAGATAAGTGT+CGG | + | Chr2:23073386-23073405 | MsG0280007926.01.T01:CDS | 40.0% | |
| ACGCTCACTTTGTGGAGAAT+AGG | - | Chr2:23073341-23073360 | None:intergenic | 45.0% | |
| AGTGCACACAGATAAGTGTC+GGG | + | Chr2:23073387-23073406 | MsG0280007926.01.T01:CDS | 45.0% | |
| CACAACTGCATGAATGCTTC+AGG | - | Chr2:23073430-23073449 | None:intergenic | 45.0% | |
| TGTCGGGACATCATACGAAT+GGG | + | Chr2:23073403-23073422 | MsG0280007926.01.T01:CDS | 45.0% | |
| CAACGTCTACGCTCACTTTG+TGG | - | Chr2:23073349-23073368 | None:intergenic | 50.0% | |
| GTCGGGACATCATACGAATG+GGG | + | Chr2:23073404-23073423 | MsG0280007926.01.T01:CDS | 50.0% | |
| GTGTCGGGACATCATACGAA+TGG | + | Chr2:23073402-23073421 | MsG0280007926.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 23073094 | 23073627 | 23073094 | ID=MsG0280007926.01;Name=MsG0280007926.01 |
| Chr2 | mRNA | 23073094 | 23073627 | 23073094 | ID=MsG0280007926.01.T01;Parent=MsG0280007926.01;Name=MsG0280007926.01.T01;_AED=0.42;_eAED=0.42;_QI=0|-1|0|1|-1|1|1|0|177 |
| Chr2 | exon | 23073094 | 23073627 | 23073094 | ID=MsG0280007926.01.T01:exon:40345;Parent=MsG0280007926.01.T01 |
| Chr2 | CDS | 23073094 | 23073627 | 23073094 | ID=MsG0280007926.01.T01:cds;Parent=MsG0280007926.01.T01 |
| Gene Sequence |
| Protein sequence |
