Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014402.01.T01 | WP_166512930.1 | 85.714 | 70 | 10 | 0 | 3 | 72 | 30 | 99 | 3.01E-35 | 125 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014402.01.T01 | Q9LDL7 | 64.286 | 70 | 25 | 0 | 3 | 72 | 407 | 476 | 1.88E-26 | 103 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014402.01.T01 | G7J211 | 90.000 | 70 | 7 | 0 | 3 | 72 | 459 | 528 | 4.14e-34 | 131 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380014402.01 | MsG0580027345.01 | 0.806649 | 7.422788e-50 | 4.050365e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014402.01.T01 | MTR_3g056110 | 90.000 | 70 | 7 | 0 | 3 | 72 | 459 | 528 | 1.05e-37 | 131 |
| MsG0380014402.01.T01 | MTR_5g094450 | 72.857 | 70 | 19 | 0 | 3 | 72 | 449 | 518 | 6.29e-31 | 112 |
| MsG0380014402.01.T01 | MTR_5g097480 | 65.714 | 70 | 24 | 0 | 3 | 72 | 461 | 530 | 6.67e-28 | 104 |
| MsG0380014402.01.T01 | MTR_2g082090 | 61.538 | 65 | 25 | 0 | 3 | 67 | 496 | 560 | 6.89e-23 | 90.5 |
| MsG0380014402.01.T01 | MTR_2g026250 | 50.000 | 66 | 33 | 0 | 3 | 68 | 513 | 578 | 3.00e-19 | 80.1 |
| MsG0380014402.01.T01 | MTR_4g133660 | 48.571 | 70 | 36 | 0 | 3 | 72 | 460 | 529 | 4.10e-19 | 79.7 |
| MsG0380014402.01.T01 | MTR_1g029420 | 46.032 | 63 | 34 | 0 | 3 | 65 | 509 | 571 | 1.39e-16 | 72.4 |
| MsG0380014402.01.T01 | MTR_3g089055 | 43.077 | 65 | 37 | 0 | 3 | 67 | 487 | 551 | 2.47e-15 | 68.9 |
| MsG0380014402.01.T01 | MTR_3g089055 | 43.077 | 65 | 37 | 0 | 3 | 67 | 482 | 546 | 2.91e-15 | 68.9 |
| MsG0380014402.01.T01 | MTR_3g022830 | 43.056 | 72 | 40 | 1 | 2 | 73 | 352 | 422 | 2.27e-12 | 60.5 |
| MsG0380014402.01.T01 | MTR_4g076140 | 41.667 | 72 | 42 | 0 | 2 | 73 | 384 | 455 | 3.92e-11 | 57.0 |
| MsG0380014402.01.T01 | MTR_3g022005 | 41.667 | 72 | 41 | 1 | 2 | 73 | 41 | 111 | 4.04e-11 | 54.7 |
| MsG0380014402.01.T01 | MTR_3g022580 | 41.667 | 72 | 41 | 1 | 2 | 73 | 99 | 169 | 6.30e-11 | 55.5 |
| MsG0380014402.01.T01 | MTR_7g057230 | 48.077 | 52 | 27 | 0 | 3 | 54 | 575 | 626 | 6.38e-11 | 56.6 |
| MsG0380014402.01.T01 | MTR_7g027190 | 44.000 | 75 | 38 | 2 | 2 | 74 | 588 | 660 | 7.46e-11 | 56.2 |
| MsG0380014402.01.T01 | MTR_6g047750 | 48.077 | 52 | 27 | 0 | 3 | 54 | 573 | 624 | 8.98e-11 | 55.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014402.01.T01 | AT5G48150 | 64.286 | 70 | 25 | 0 | 3 | 72 | 407 | 476 | 1.91e-27 | 103 |
| MsG0380014402.01.T01 | AT5G48150 | 64.286 | 70 | 25 | 0 | 3 | 72 | 407 | 476 | 1.91e-27 | 103 |
| MsG0380014402.01.T01 | AT5G48150 | 64.286 | 70 | 25 | 0 | 3 | 72 | 407 | 476 | 1.91e-27 | 103 |
| MsG0380014402.01.T01 | AT5G48150 | 64.286 | 70 | 25 | 0 | 3 | 72 | 407 | 476 | 1.91e-27 | 103 |
| MsG0380014402.01.T01 | AT1G50600 | 68.571 | 70 | 22 | 0 | 3 | 72 | 514 | 583 | 3.83e-27 | 102 |
| MsG0380014402.01.T01 | AT1G50600 | 68.571 | 70 | 22 | 0 | 3 | 72 | 518 | 587 | 3.84e-27 | 102 |
| MsG0380014402.01.T01 | AT1G50600 | 68.571 | 70 | 22 | 0 | 3 | 72 | 443 | 512 | 3.94e-27 | 102 |
| MsG0380014402.01.T01 | AT2G04890 | 60.000 | 70 | 28 | 0 | 3 | 72 | 330 | 399 | 8.80e-26 | 97.8 |
| MsG0380014402.01.T01 | AT1G21450 | 50.000 | 70 | 32 | 2 | 3 | 69 | 509 | 578 | 1.12e-17 | 75.5 |
| MsG0380014402.01.T01 | AT1G66350 | 47.297 | 74 | 36 | 2 | 3 | 74 | 421 | 493 | 9.22e-16 | 70.1 |
| MsG0380014402.01.T01 | AT4G17230 | 42.857 | 70 | 40 | 0 | 3 | 72 | 442 | 511 | 2.70e-14 | 66.2 |
| MsG0380014402.01.T01 | AT4G17230 | 42.857 | 70 | 40 | 0 | 3 | 72 | 442 | 511 | 2.70e-14 | 66.2 |
| MsG0380014402.01.T01 | AT5G52510 | 42.857 | 63 | 36 | 0 | 3 | 65 | 557 | 619 | 1.67e-11 | 58.2 |
| MsG0380014402.01.T01 | AT5G17490 | 43.284 | 67 | 36 | 1 | 3 | 67 | 431 | 497 | 2.11e-11 | 57.8 |
| MsG0380014402.01.T01 | AT1G14920 | 39.189 | 74 | 42 | 2 | 3 | 74 | 444 | 516 | 3.79e-11 | 57.0 |
| MsG0380014402.01.T01 | AT2G01570 | 40.541 | 74 | 41 | 2 | 3 | 74 | 496 | 568 | 6.15e-11 | 56.6 |
Find 16 sgRNAs with CRISPR-Local
Find 17 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTGCACCTCTCTCTCTTCT+AGG | 0.319437 | 3:+54194733 | None:intergenic |
| GAGAGAGGTGCACTGTTTCT+TGG | 0.333491 | 3:-54194724 | MsG0380014402.01.T01:CDS |
| CTTGATTGAAGAATTGATGA+AGG | 0.426007 | 3:+54194788 | None:intergenic |
| ATGAAGGAGCTCAATGGATA+AGG | 0.465572 | 3:+54194804 | None:intergenic |
| GAGGTGCACTGTTTCTTGGT+TGG | 0.490660 | 3:-54194720 | MsG0380014402.01.T01:CDS |
| GTCACGAGCTTCTGAGTAAG+TGG | 0.507829 | 3:-54194857 | MsG0380014402.01.T01:CDS |
| GTGCACCTCTCTCTCTTCTA+GGG | 0.513222 | 3:+54194734 | None:intergenic |
| TGAACTTAGTAGCTTGCGAA+GGG | 0.564647 | 3:-54194896 | MsG0380014402.01.T01:CDS |
| TTAATGTCGAACAGCACTGT+TGG | 0.576640 | 3:-54194931 | None:intergenic |
| GTGAACTTAGTAGCTTGCGA+AGG | 0.579196 | 3:-54194897 | MsG0380014402.01.T01:CDS |
| TTACACCCTAGAAGAGAGAG+AGG | 0.616707 | 3:-54194739 | MsG0380014402.01.T01:CDS |
| CTTCTAGGGTGTAATGTCCA+CGG | 0.620198 | 3:+54194748 | None:intergenic |
| GAATTGATGAAGGAGCTCAA+TGG | 0.624450 | 3:+54194798 | None:intergenic |
| AATCTTCTCGAGAGCTACCG+TGG | 0.627998 | 3:-54194765 | MsG0380014402.01.T01:CDS |
| TTGCGAAGGGGAAGAAAGAG+TGG | 0.653404 | 3:-54194883 | MsG0380014402.01.T01:CDS |
| GAACTTAGTAGCTTGCGAAG+GGG | 0.697810 | 3:-54194895 | MsG0380014402.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CTTGATTGAAGAATTGATGA+AGG | + | Chr3:54194849-54194868 | None:intergenic | 30.0% | |
| ATGAAGGAGCTCAATGGATA+AGG | + | Chr3:54194833-54194852 | None:intergenic | 40.0% | |
| GAATTGATGAAGGAGCTCAA+TGG | + | Chr3:54194839-54194858 | None:intergenic | 40.0% | |
| ! | TGAACTTAGTAGCTTGCGAA+GGG | - | Chr3:54194738-54194757 | MsG0380014402.01.T01:CDS | 40.0% |
| TTACACCCTAGAAGAGAGAG+AGG | - | Chr3:54194895-54194914 | MsG0380014402.01.T01:CDS | 45.0% | |
| ! | GAACTTAGTAGCTTGCGAAG+GGG | - | Chr3:54194739-54194758 | MsG0380014402.01.T01:CDS | 45.0% |
| ! | GTGAACTTAGTAGCTTGCGA+AGG | - | Chr3:54194737-54194756 | MsG0380014402.01.T01:CDS | 45.0% |
| !! | CTTCTAGGGTGTAATGTCCA+CGG | + | Chr3:54194889-54194908 | None:intergenic | 45.0% |
| !!! | AAAAAGCGTTTTGCGATGGC+TGG | - | Chr3:54194800-54194819 | MsG0380014402.01.T01:CDS | 45.0% |
| !!! | GTGGAAAAAGCGTTTTGCGA+TGG | - | Chr3:54194796-54194815 | MsG0380014402.01.T01:CDS | 45.0% |
| AATCTTCTCGAGAGCTACCG+TGG | - | Chr3:54194869-54194888 | MsG0380014402.01.T01:CDS | 50.0% | |
| AGTGCACCTCTCTCTCTTCT+AGG | + | Chr3:54194904-54194923 | None:intergenic | 50.0% | |
| GTCACGAGCTTCTGAGTAAG+TGG | - | Chr3:54194777-54194796 | MsG0380014402.01.T01:CDS | 50.0% | |
| GTGCACCTCTCTCTCTTCTA+GGG | + | Chr3:54194903-54194922 | None:intergenic | 50.0% | |
| TTGCGAAGGGGAAGAAAGAG+TGG | - | Chr3:54194751-54194770 | MsG0380014402.01.T01:CDS | 50.0% | |
| ! | GAGAGAGGTGCACTGTTTCT+TGG | - | Chr3:54194910-54194929 | MsG0380014402.01.T01:CDS | 50.0% |
| ! | GAGGTGCACTGTTTCTTGGT+TGG | - | Chr3:54194914-54194933 | MsG0380014402.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 54194716 | 54194940 | 54194716 | ID=MsG0380014402.01;Name=MsG0380014402.01 |
| Chr3 | mRNA | 54194716 | 54194940 | 54194716 | ID=MsG0380014402.01.T01;Parent=MsG0380014402.01;Name=MsG0380014402.01.T01;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|0|1|0|74 |
| Chr3 | exon | 54194716 | 54194940 | 54194716 | ID=MsG0380014402.01.T01:exon:10920;Parent=MsG0380014402.01.T01 |
| Chr3 | CDS | 54194716 | 54194940 | 54194716 | ID=MsG0380014402.01.T01:cds;Parent=MsG0380014402.01.T01 |
| Gene Sequence |
| Protein sequence |
