Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014439.01.T01 | XP_003600328.1 | 92.347 | 392 | 26 | 1 | 16 | 403 | 107 | 498 | 0 | 755 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180004110.01 | MsG0380014439.01 | 0.800796 | 1.217972e-48 | 5.722912e-46 |
| MsG0380014439.01 | MsG0480021549.01 | 0.864935 | 8.495760e-65 | 2.714556e-61 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014439.01.T01 | MTR_3g057980 | 92.347 | 392 | 26 | 1 | 16 | 403 | 107 | 498 | 0.0 | 755 |
| MsG0380014439.01.T01 | MTR_3g057900 | 82.199 | 382 | 67 | 1 | 23 | 403 | 118 | 499 | 0.0 | 660 |
| MsG0380014439.01.T01 | MTR_3g057970 | 79.843 | 382 | 73 | 2 | 23 | 403 | 115 | 493 | 0.0 | 637 |
| MsG0380014439.01.T01 | MTR_3g058210 | 60.881 | 386 | 143 | 3 | 25 | 403 | 121 | 505 | 5.54e-179 | 508 |
| MsG0380014439.01.T01 | MTR_3g058220 | 61.257 | 382 | 139 | 4 | 28 | 402 | 123 | 502 | 6.64e-173 | 493 |
| MsG0380014439.01.T01 | MTR_3g058380 | 59.530 | 383 | 146 | 3 | 28 | 403 | 43 | 423 | 2.11e-172 | 488 |
| MsG0380014439.01.T01 | MTR_1g035140 | 60.309 | 388 | 139 | 6 | 25 | 403 | 121 | 502 | 2.77e-172 | 491 |
| MsG0380014439.01.T01 | MTR_3g058230 | 58.549 | 386 | 144 | 4 | 25 | 403 | 49 | 425 | 1.47e-170 | 484 |
| MsG0380014439.01.T01 | MTR_3g058240 | 60.363 | 386 | 145 | 3 | 25 | 403 | 85 | 469 | 4.73e-170 | 484 |
| MsG0380014439.01.T01 | MTR_3g058250 | 60.622 | 386 | 144 | 3 | 25 | 403 | 119 | 503 | 9.98e-169 | 482 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014439.01.T01 | AT3G26300 | 41.422 | 408 | 209 | 6 | 10 | 401 | 104 | 497 | 6.38e-106 | 322 |
| MsG0380014439.01.T01 | AT3G26320 | 39.440 | 393 | 213 | 5 | 25 | 401 | 114 | 497 | 2.48e-104 | 318 |
| MsG0380014439.01.T01 | AT3G26310 | 40.561 | 392 | 208 | 5 | 25 | 400 | 113 | 495 | 2.32e-103 | 315 |
| MsG0380014439.01.T01 | AT3G26330 | 40.204 | 393 | 210 | 5 | 25 | 401 | 114 | 497 | 5.47e-102 | 311 |
| MsG0380014439.01.T01 | AT3G26290 | 42.674 | 389 | 198 | 6 | 29 | 401 | 118 | 497 | 1.74e-97 | 300 |
| MsG0380014439.01.T01 | AT3G26290 | 42.674 | 389 | 198 | 6 | 29 | 401 | 131 | 510 | 2.14e-97 | 300 |
| MsG0380014439.01.T01 | AT3G26230 | 40.204 | 393 | 217 | 8 | 25 | 402 | 109 | 498 | 8.84e-97 | 298 |
| MsG0380014439.01.T01 | AT5G57260 | 39.340 | 394 | 212 | 6 | 25 | 399 | 113 | 498 | 4.66e-96 | 296 |
| MsG0380014439.01.T01 | AT5G25120 | 39.796 | 392 | 209 | 6 | 25 | 400 | 112 | 492 | 1.53e-95 | 295 |
| MsG0380014439.01.T01 | AT2G02580 | 41.624 | 394 | 203 | 6 | 25 | 401 | 114 | 497 | 2.66e-95 | 294 |
Find 63 sgRNAs with CRISPR-Local
Find 201 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACATTATTGATGAACAT+AGG | 0.528089 | 3:+54904576 | MsG0380014439.01.T01:CDS |
| AAAACCAAATGAAGGTGACA+TGG | 0.602504 | 3:+54906026 | MsG0380014439.01.T01:CDS |
| AAACATTATTGATGAACATA+GGG | 0.515452 | 3:+54904577 | MsG0380014439.01.T01:CDS |
| AAAGAAAGAAAACCAAATGA+AGG | 0.537141 | 3:+54906018 | MsG0380014439.01.T01:CDS |
| AAATGGCTTCAAAATATATC+TGG | 0.289129 | 3:+54904503 | MsG0380014439.01.T01:CDS |
| AAGTTGATTGCTTCAAAAGA+AGG | 0.449405 | 3:+54904314 | MsG0380014439.01.T01:CDS |
| AATGGAGAAAGTACAAGCTG+AGG | 0.638477 | 3:+54905978 | MsG0380014439.01.T01:CDS |
| ACAAATACATGATATATGTA+AGG | 0.502167 | 3:+54903195 | MsG0380014439.01.T01:CDS |
| ACCAAACCAAATGTGACTCC+AGG | 0.557520 | 3:-54906330 | None:intergenic |
| AGAAATTCATATCAATTGTA+AGG | 0.389275 | 3:+54904426 | MsG0380014439.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAACGGAATTAGAGTAGTAT+TGG | + | Chr3:54905575-54905594 | MsG0380014439.01.T01:intron | 30.0% | |
| AATAGAGTTAGAGCTAGTAT+TGG | + | Chr3:54905153-54905172 | MsG0380014439.01.T01:intron | 30.0% | |
| AATTCTCTTAGCTTATGATG+AGG | - | Chr3:54904003-54904022 | None:intergenic | 30.0% | |
| AATTTGCCTACTATTATAGG+GGG | + | Chr3:54903283-54903302 | MsG0380014439.01.T01:intron | 30.0% | |
| ATAGAAATTTGAGAAAGCCA+CGG | + | Chr3:54905821-54905840 | MsG0380014439.01.T01:intron | 30.0% | |
| ATAGGGAGGTTAATAAATCA+CGG | + | Chr3:54904594-54904613 | MsG0380014439.01.T01:CDS | 30.0% | |
| ATTAGTAAATTGAGGATACG+TGG | - | Chr3:54903710-54903729 | None:intergenic | 30.0% | |
| ATTTGTACCCTTCTTGTAAA+TGG | + | Chr3:54904486-54904505 | MsG0380014439.01.T01:CDS | 30.0% | |
| CAACTTTCAATAAACCTTTC+AGG | - | Chr3:54906249-54906268 | None:intergenic | 30.0% | |
| CTATAAACACAAACATGAGA+AGG | - | Chr3:54903873-54903892 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 54903140 | 54906509 | 54903140 | ID=MsG0380014439.01;Name=MsG0380014439.01 |
| Chr3 | mRNA | 54903140 | 54906509 | 54903140 | ID=MsG0380014439.01.T01;Parent=MsG0380014439.01;Name=MsG0380014439.01.T01;_AED=0.35;_eAED=0.35;_QI=0|0|0|0.66|1|1|3|0|403 |
| Chr3 | exon | 54903140 | 54903216 | 54903140 | ID=MsG0380014439.01.T01:exon:30365;Parent=MsG0380014439.01.T01 |
| Chr3 | exon | 54904193 | 54904715 | 54904193 | ID=MsG0380014439.01.T01:exon:30366;Parent=MsG0380014439.01.T01 |
| Chr3 | exon | 54905898 | 54906509 | 54905898 | ID=MsG0380014439.01.T01:exon:30367;Parent=MsG0380014439.01.T01 |
| Chr3 | CDS | 54903140 | 54903216 | 54903140 | ID=MsG0380014439.01.T01:cds;Parent=MsG0380014439.01.T01 |
| Chr3 | CDS | 54904193 | 54904715 | 54904193 | ID=MsG0380014439.01.T01:cds;Parent=MsG0380014439.01.T01 |
| Chr3 | CDS | 54905898 | 54906509 | 54905898 | ID=MsG0380014439.01.T01:cds;Parent=MsG0380014439.01.T01 |
| Gene Sequence |
>MsG0380014439.01.T01 GTGAAAGTGGCTATTAAGAATTTGCGTGCTCGTGAGCTTAGCTCAGTTGATAGAAACAAATACATGATATATGTAAGGTTTGGACCTTATGGTGATTATTGGAGAAACCTTAGAAAAATTTGTACACTTGAGCTATTAAGTTCAAAACGTGTCCAATCTTTTCAACCAATTAGAAGTGAAGAGGTAACTAATCTAATCAAGTTGATTGCTTCAAAAGAAGGATCACAAATCAATTTTACCAAAGAAATTTTTTCAACAATTTCTGCAATCACTTCAAGAACAGCTTTTGGGAAAAAGTGTAAGGAAAATCAGAAATTCATATCAATTGTAAGGGATGCTATTAAGATTGCTGGTGGTTTTGATTTGGGAGATTTGTACCCTTCTTGTAAATGGCTTCAAAATATATCTGGATTGAAGCCTAAGCTTGAAAAGTTGCACAAACAAGCTGATTTGATTATGCAAAACATTATTGATGAACATAGGGAGGTTAATAAATCACGGGTTAATGAAGATTGTGGTGAAGAACTGGAGGAAGATCTTGTAGATGTACTCTTGAAGCAAGATTTTTTGAGGGATAATAGTGTCAAAGCCGTGATCTTGGATATGTATGGAGGTGGAAGTGAGACATCAGCTACAAGCATAACATGGGCGATGGCAGAGATGATTAAAAATCCCACAATAATGGAGAAAGTACAAGCTGAGGTAAGAGAGGTATTTGATAAAGAAAGAAAACCAAATGAAGGTGACATGGAGAAATTAACATATTTAAAATATGTTGTGAAAGAGACATTGAGATTACACCCTCCTGCTGCATTTTTGCTTCCAAGAGAATGTGGACAAGCATGTGAGATAAATGGATATGGCATACCTTTTAAAAGCAAAGTGATAGTGAATGCTTGGGCCATCGGAAGAGATCCAAATCATTGGGATGACCCTGAGAGATTTTATCCTGAAAGGTTTATTGAAAGTTGTGTAGATTATAAGGGTAATAATTTTGAGTTCGTTCCATTTGGTGCTGGAAGAAGAATGTGTCCTGGAGTCACATTTGGTTTGGTCAATGTTGAGTATCCACTTGCATTGTTGATGTATCACTTTGACTGGAAACTTCCCAATGAGATGAAAAATGAAGATTTGGACATGAGTGAAACTTTTGGATCAGCAGTTACTCGAAAAGACGATTTATACCTTATTCCAGTCATGTATCATCCTTAG |
| Protein sequence |
>MsG0380014439.01.T01 VKVAIKNLRARELSSVDRNKYMIYVRFGPYGDYWRNLRKICTLELLSSKRVQSFQPIRSEEVTNLIKLIASKEGSQINFTKEIFSTISAITSRTAFGKKCKENQKFISIVRDAIKIAGGFDLGDLYPSCKWLQNISGLKPKLEKLHKQADLIMQNIIDEHREVNKSRVNEDCGEELEEDLVDVLLKQDFLRDNSVKAVILDMYGGGSETSATSITWAMAEMIKNPTIMEKVQAEVREVFDKERKPNEGDMEKLTYLKYVVKETLRLHPPAAFLLPRECGQACEINGYGIPFKSKVIVNAWAIGRDPNHWDDPERFYPERFIESCVDYKGNNFEFVPFGAGRRMCPGVTFGLVNVEYPLALLMYHFDWKLPNEMKNEDLDMSETFGSAVTRKDDLYLIPVMYHP* |
