Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014572.01.T01 | XP_024640479.2 | 58.088 | 136 | 56 | 1 | 2 | 136 | 109 | 244 | 9.17E-46 | 159 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014572.01.T01 | A0A396GZG8 | 57.778 | 135 | 56 | 1 | 2 | 135 | 127 | 261 | 2.40e-45 | 157 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0380014572.01 | MsG0280009322.01 | PPI |
| MsG0380014572.01 | MsG0280010449.01 | PPI |
| MsG0380014572.01 | MsG0780041116.01 | PPI |
| MsG0380014572.01 | MsG0780041116.01 | PPI |
| MsG0380014572.01 | MsG0780040274.01 | PPI |
| MsG0380014572.01 | MsG0380013578.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014572.01.T01 | MTR_7g082560 | 61.905 | 126 | 16 | 2 | 6 | 130 | 10 | 104 | 4.61e-44 | 140 |
| MsG0380014572.01.T01 | MTR_5g091440 | 44.628 | 121 | 35 | 1 | 10 | 130 | 3 | 91 | 2.99e-26 | 95.9 |
| MsG0380014572.01.T01 | MTR_2g027020 | 42.308 | 130 | 70 | 2 | 2 | 130 | 28 | 153 | 3.49e-26 | 97.4 |
| MsG0380014572.01.T01 | MTR_7g063280 | 41.748 | 103 | 59 | 1 | 2 | 103 | 72 | 174 | 3.38e-25 | 94.7 |
| MsG0380014572.01.T01 | MTR_6g016265 | 54.321 | 81 | 36 | 1 | 4 | 83 | 41 | 121 | 6.03e-24 | 90.1 |
| MsG0380014572.01.T01 | MTR_8g036780 | 51.765 | 85 | 31 | 1 | 1 | 85 | 33 | 107 | 2.71e-23 | 87.8 |
| MsG0380014572.01.T01 | MTR_6g034915 | 37.405 | 131 | 66 | 1 | 3 | 133 | 56 | 170 | 1.13e-20 | 82.8 |
| MsG0380014572.01.T01 | MTR_8g088220 | 53.425 | 73 | 33 | 1 | 12 | 83 | 4 | 76 | 2.43e-20 | 79.3 |
| MsG0380014572.01.T01 | MTR_8g059135 | 48.352 | 91 | 46 | 1 | 5 | 94 | 31 | 121 | 3.51e-20 | 80.5 |
| MsG0380014572.01.T01 | MTR_0015s0120 | 52.000 | 75 | 36 | 0 | 1 | 75 | 292 | 366 | 7.55e-20 | 84.0 |
| MsG0380014572.01.T01 | MTR_5g034450 | 56.250 | 64 | 28 | 0 | 2 | 65 | 49 | 112 | 1.88e-19 | 78.2 |
| MsG0380014572.01.T01 | MTR_1g088650 | 42.157 | 102 | 58 | 1 | 4 | 104 | 88 | 189 | 2.68e-19 | 79.7 |
| MsG0380014572.01.T01 | MTR_4g008270 | 43.590 | 78 | 44 | 0 | 3 | 80 | 11 | 88 | 5.09e-19 | 76.3 |
| MsG0380014572.01.T01 | MTR_7g013520 | 33.083 | 133 | 65 | 1 | 1 | 133 | 69 | 177 | 4.20e-18 | 76.6 |
| MsG0380014572.01.T01 | MTR_1g081930 | 41.304 | 92 | 53 | 1 | 1 | 91 | 37 | 128 | 5.83e-18 | 74.7 |
| MsG0380014572.01.T01 | MTR_5g049680 | 50.000 | 68 | 34 | 0 | 1 | 68 | 30 | 97 | 7.51e-18 | 73.9 |
| MsG0380014572.01.T01 | MTR_8g031800 | 41.667 | 84 | 49 | 0 | 1 | 84 | 30 | 113 | 1.26e-17 | 73.6 |
| MsG0380014572.01.T01 | MTR_5g075610 | 41.414 | 99 | 55 | 2 | 8 | 105 | 1 | 97 | 2.89e-17 | 72.0 |
| MsG0380014572.01.T01 | MTR_8g099580 | 51.948 | 77 | 37 | 0 | 2 | 78 | 206 | 282 | 3.25e-17 | 76.3 |
| MsG0380014572.01.T01 | MTR_1g074090 | 46.269 | 67 | 36 | 0 | 2 | 68 | 81 | 147 | 5.20e-17 | 72.8 |
| MsG0380014572.01.T01 | MTR_1g074240 | 52.857 | 70 | 32 | 1 | 4 | 72 | 2 | 71 | 6.93e-17 | 70.5 |
| MsG0380014572.01.T01 | MTR_5g036320 | 53.030 | 66 | 31 | 0 | 2 | 67 | 79 | 144 | 7.51e-17 | 72.4 |
| MsG0380014572.01.T01 | MTR_6g453270 | 45.455 | 77 | 42 | 0 | 2 | 78 | 300 | 376 | 1.18e-16 | 75.1 |
| MsG0380014572.01.T01 | MTR_2g059470 | 45.455 | 77 | 42 | 0 | 4 | 80 | 2 | 78 | 1.39e-16 | 70.1 |
| MsG0380014572.01.T01 | MTR_1g069475 | 42.500 | 80 | 46 | 0 | 1 | 80 | 163 | 242 | 2.04e-16 | 73.2 |
| MsG0380014572.01.T01 | MTR_1g045560 | 34.579 | 107 | 55 | 1 | 8 | 114 | 1 | 92 | 2.47e-16 | 69.7 |
| MsG0380014572.01.T01 | MTR_7g053400 | 37.383 | 107 | 34 | 1 | 6 | 112 | 179 | 252 | 5.68e-16 | 72.4 |
| MsG0380014572.01.T01 | MTR_6g089560 | 42.857 | 77 | 44 | 0 | 2 | 78 | 116 | 192 | 1.38e-15 | 70.9 |
| MsG0380014572.01.T01 | MTR_1g062840 | 44.595 | 74 | 40 | 1 | 24 | 96 | 2 | 75 | 2.58e-15 | 67.0 |
| MsG0380014572.01.T01 | MTR_3g116210 | 39.286 | 84 | 50 | 1 | 1 | 83 | 96 | 179 | 3.24e-15 | 68.9 |
| MsG0380014572.01.T01 | MTR_0093s0070 | 45.000 | 80 | 43 | 1 | 5 | 83 | 126 | 205 | 3.84e-15 | 69.3 |
| MsG0380014572.01.T01 | MTR_8g038340 | 47.458 | 59 | 31 | 0 | 1 | 59 | 188 | 246 | 6.27e-15 | 69.3 |
| MsG0380014572.01.T01 | MTR_1g050488 | 49.231 | 65 | 33 | 0 | 1 | 65 | 97 | 161 | 1.29e-14 | 67.0 |
| MsG0380014572.01.T01 | MTR_4g094840 | 57.143 | 56 | 17 | 1 | 54 | 109 | 1 | 49 | 1.87e-14 | 64.7 |
| MsG0380014572.01.T01 | MTR_5g085210 | 37.931 | 116 | 57 | 3 | 1 | 116 | 118 | 218 | 1.91e-14 | 67.8 |
| MsG0380014572.01.T01 | MTR_1g017840 | 40.000 | 80 | 48 | 0 | 2 | 81 | 78 | 157 | 5.09e-14 | 65.5 |
| MsG0380014572.01.T01 | MTR_8g098925 | 40.426 | 94 | 55 | 1 | 6 | 98 | 69 | 162 | 5.65e-14 | 65.1 |
| MsG0380014572.01.T01 | MTR_5g037920 | 33.594 | 128 | 76 | 4 | 6 | 128 | 102 | 225 | 8.41e-14 | 65.9 |
| MsG0380014572.01.T01 | MTR_3g090920 | 32.773 | 119 | 65 | 3 | 17 | 135 | 2 | 105 | 2.13e-13 | 62.4 |
| MsG0380014572.01.T01 | MTR_1g022215 | 49.123 | 57 | 29 | 0 | 2 | 58 | 14 | 70 | 3.94e-13 | 61.2 |
| MsG0380014572.01.T01 | MTR_6g016135 | 51.667 | 60 | 29 | 0 | 6 | 65 | 383 | 442 | 7.68e-13 | 64.7 |
| MsG0380014572.01.T01 | MTR_5g069700 | 40.909 | 66 | 38 | 1 | 23 | 87 | 2 | 67 | 1.01e-12 | 59.7 |
| MsG0380014572.01.T01 | MTR_5g083200 | 43.421 | 76 | 43 | 0 | 4 | 79 | 100 | 175 | 1.42e-12 | 62.4 |
| MsG0380014572.01.T01 | MTR_0002s1220 | 43.421 | 76 | 43 | 0 | 4 | 79 | 100 | 175 | 1.42e-12 | 62.4 |
| MsG0380014572.01.T01 | MTR_4g064590 | 29.921 | 127 | 82 | 3 | 10 | 133 | 34 | 156 | 2.67e-12 | 60.8 |
| MsG0380014572.01.T01 | MTR_1g045340 | 42.105 | 76 | 44 | 0 | 4 | 79 | 86 | 161 | 4.26e-12 | 60.8 |
| MsG0380014572.01.T01 | MTR_3g111390 | 42.105 | 76 | 44 | 0 | 4 | 79 | 100 | 175 | 4.82e-12 | 60.8 |
| MsG0380014572.01.T01 | MTR_1g070820 | 42.105 | 76 | 44 | 0 | 4 | 79 | 100 | 175 | 4.82e-12 | 60.8 |
| MsG0380014572.01.T01 | MTR_7g072030 | 43.636 | 55 | 31 | 0 | 21 | 75 | 68 | 122 | 5.23e-12 | 59.3 |
| MsG0380014572.01.T01 | MTR_4g098670 | 40.000 | 80 | 47 | 1 | 57 | 135 | 16 | 95 | 1.69e-11 | 57.0 |
| MsG0380014572.01.T01 | MTR_8g028785 | 45.570 | 79 | 42 | 1 | 31 | 108 | 287 | 365 | 1.69e-11 | 60.5 |
| MsG0380014572.01.T01 | MTR_1g034430 | 34.091 | 88 | 57 | 1 | 1 | 87 | 94 | 181 | 2.03e-11 | 58.9 |
| MsG0380014572.01.T01 | MTR_4g029360 | 36.634 | 101 | 63 | 1 | 2 | 101 | 127 | 227 | 2.24e-11 | 59.3 |
| MsG0380014572.01.T01 | MTR_8g079540 | 45.161 | 62 | 34 | 0 | 2 | 63 | 48 | 109 | 2.50e-11 | 57.4 |
| MsG0380014572.01.T01 | MTR_6g016355 | 43.548 | 62 | 35 | 0 | 2 | 63 | 116 | 177 | 3.13e-11 | 58.5 |
| MsG0380014572.01.T01 | MTR_1g109000 | 49.153 | 59 | 30 | 0 | 4 | 62 | 28 | 86 | 3.35e-11 | 56.2 |
| MsG0380014572.01.T01 | MTR_3g026270 | 48.077 | 52 | 27 | 0 | 10 | 61 | 3 | 54 | 3.61e-11 | 55.5 |
| MsG0380014572.01.T01 | MTR_1g017820 | 44.444 | 63 | 34 | 1 | 14 | 76 | 12 | 73 | 5.43e-11 | 55.5 |
| MsG0380014572.01.T01 | MTR_4g010930 | 51.724 | 58 | 27 | 1 | 43 | 99 | 1 | 58 | 6.61e-11 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 30 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGATCTACAGTTGGTGAATA+TGG | 0.285393 | 3:+56631504 | MsG0380014572.01.T01:CDS |
| TTGTGTTAGCTTGGACTGAC+TGG | 0.382739 | 3:+56631413 | MsG0380014572.01.T01:CDS |
| TGCTCATAGCTTAACTAAGA+TGG | 0.429469 | 3:+56631681 | MsG0380014572.01.T01:CDS |
| ACTCTGATGTGAAGTTCATT+AGG | 0.435545 | 3:+56631641 | MsG0380014572.01.T01:CDS |
| AACATTTACAGAACGCAATC+TGG | 0.447664 | 3:+56631559 | MsG0380014572.01.T01:CDS |
| GGCTATTACAAGCTATGGAC+TGG | 0.448185 | 3:+56631476 | MsG0380014572.01.T01:CDS |
| ATGAAATGTTATAATGAATA+CGG | 0.448735 | 3:-56631723 | None:intergenic |
| AGTAAATGTATACACATGTT+AGG | 0.450990 | 3:+56631604 | MsG0380014572.01.T01:CDS |
| TAATAGCCCGAGAGCTTCCC+TGG | 0.451504 | 3:-56631461 | None:intergenic |
| TGCGTTCTGTAAATGTTGTC+CGG | 0.473920 | 3:-56631553 | None:intergenic |
| CTGGCATCAACATCAATGAT+AGG | 0.474433 | 3:-56631442 | None:intergenic |
| TCTGCATTAGAGACGCGAAT+GGG | 0.485047 | 3:+56631386 | MsG0380014572.01.T01:CDS |
| AATGTTGTCCGGTACCGTCT+TGG | 0.491886 | 3:-56631542 | None:intergenic |
| GTTGATGCCAGGGAAGCTCT+CGG | 0.495039 | 3:+56631454 | MsG0380014572.01.T01:CDS |
| CTATCATTGATGTTGATGCC+AGG | 0.519883 | 3:+56631443 | MsG0380014572.01.T01:CDS |
| GTTGGTGAATATGGATTTCG+AGG | 0.526618 | 3:+56631513 | MsG0380014572.01.T01:CDS |
| TCTCGGGCTATTACAAGCTA+TGG | 0.526899 | 3:+56631471 | MsG0380014572.01.T01:CDS |
| TTGATGCCAGGGAAGCTCTC+GGG | 0.547879 | 3:+56631455 | MsG0380014572.01.T01:CDS |
| CAGAACGCAATCTGGTGTAT+CGG | 0.561000 | 3:+56631567 | MsG0380014572.01.T01:CDS |
| ACAAGCTATGGACTGGGTGA+AGG | 0.570831 | 3:+56631483 | MsG0380014572.01.T01:CDS |
| GGTGGATTCCAAGACGGTAC+CGG | 0.576981 | 3:+56631534 | MsG0380014572.01.T01:CDS |
| TATCATTGATGTTGATGCCA+GGG | 0.587305 | 3:+56631444 | MsG0380014572.01.T01:CDS |
| TCGGAAAGTATGGACAGAGT+GGG | 0.587642 | 3:+56631355 | None:intergenic |
| GCTATTACAAGCTATGGACT+GGG | 0.599559 | 3:+56631477 | MsG0380014572.01.T01:CDS |
| ATCTGCATTAGAGACGCGAA+TGG | 0.611646 | 3:+56631385 | MsG0380014572.01.T01:CDS |
| TTCGGAAAGTATGGACAGAG+TGG | 0.619158 | 3:+56631354 | None:intergenic |
| CTGCATTAGAGACGCGAATG+GGG | 0.629771 | 3:+56631387 | MsG0380014572.01.T01:CDS |
| TTTCGAGGTGGATTCCAAGA+CGG | 0.635793 | 3:+56631528 | MsG0380014572.01.T01:CDS |
| GGTGAATATGGATTTCGAGG+TGG | 0.651187 | 3:+56631516 | MsG0380014572.01.T01:CDS |
| CTGGGTGAAGGATCTACAGT+TGG | 0.667590 | 3:+56631495 | MsG0380014572.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGAAATGTTATAATGAATA+CGG | - | Chr3:56631726-56631745 | None:intergenic | 15.0% |
| ! | AGTAAATGTATACACATGTT+AGG | + | Chr3:56631604-56631623 | MsG0380014572.01.T01:CDS | 25.0% |
| AACATTTACAGAACGCAATC+TGG | + | Chr3:56631559-56631578 | MsG0380014572.01.T01:CDS | 35.0% | |
| ACTCTGATGTGAAGTTCATT+AGG | + | Chr3:56631641-56631660 | MsG0380014572.01.T01:CDS | 35.0% | |
| TATCATTGATGTTGATGCCA+GGG | + | Chr3:56631444-56631463 | MsG0380014572.01.T01:CDS | 35.0% | |
| TGCTCATAGCTTAACTAAGA+TGG | + | Chr3:56631681-56631700 | MsG0380014572.01.T01:CDS | 35.0% | |
| CTATCATTGATGTTGATGCC+AGG | + | Chr3:56631443-56631462 | MsG0380014572.01.T01:CDS | 40.0% | |
| CTGGCATCAACATCAATGAT+AGG | - | Chr3:56631445-56631464 | None:intergenic | 40.0% | |
| GCTATTACAAGCTATGGACT+GGG | + | Chr3:56631477-56631496 | MsG0380014572.01.T01:CDS | 40.0% | |
| TGCGTTCTGTAAATGTTGTC+CGG | - | Chr3:56631556-56631575 | None:intergenic | 40.0% | |
| ! | GTTGGTGAATATGGATTTCG+AGG | + | Chr3:56631513-56631532 | MsG0380014572.01.T01:CDS | 40.0% |
| !! | GGATCTACAGTTGGTGAATA+TGG | + | Chr3:56631504-56631523 | MsG0380014572.01.T01:CDS | 40.0% |
| ATCTGCATTAGAGACGCGAA+TGG | + | Chr3:56631385-56631404 | MsG0380014572.01.T01:CDS | 45.0% | |
| CAGAACGCAATCTGGTGTAT+CGG | + | Chr3:56631567-56631586 | MsG0380014572.01.T01:CDS | 45.0% | |
| GGCTATTACAAGCTATGGAC+TGG | + | Chr3:56631476-56631495 | MsG0380014572.01.T01:CDS | 45.0% | |
| GGTGAATATGGATTTCGAGG+TGG | + | Chr3:56631516-56631535 | MsG0380014572.01.T01:CDS | 45.0% | |
| TCTCGGGCTATTACAAGCTA+TGG | + | Chr3:56631471-56631490 | MsG0380014572.01.T01:CDS | 45.0% | |
| TCTGCATTAGAGACGCGAAT+GGG | + | Chr3:56631386-56631405 | MsG0380014572.01.T01:CDS | 45.0% | |
| ! | ATGGGGCTTTTGTGTTAGCT+TGG | + | Chr3:56631404-56631423 | MsG0380014572.01.T01:CDS | 45.0% |
| ! | TTGTGTTAGCTTGGACTGAC+TGG | + | Chr3:56631413-56631432 | MsG0380014572.01.T01:CDS | 45.0% |
| ! | TTTCGAGGTGGATTCCAAGA+CGG | + | Chr3:56631528-56631547 | MsG0380014572.01.T01:CDS | 45.0% |
| AATGTTGTCCGGTACCGTCT+TGG | - | Chr3:56631545-56631564 | None:intergenic | 50.0% | |
| CTGCATTAGAGACGCGAATG+GGG | + | Chr3:56631387-56631406 | MsG0380014572.01.T01:CDS | 50.0% | |
| CTGGGTGAAGGATCTACAGT+TGG | + | Chr3:56631495-56631514 | MsG0380014572.01.T01:CDS | 50.0% | |
| !! | ACAAGCTATGGACTGGGTGA+AGG | + | Chr3:56631483-56631502 | MsG0380014572.01.T01:CDS | 50.0% |
| GTTGATGCCAGGGAAGCTCT+CGG | + | Chr3:56631454-56631473 | MsG0380014572.01.T01:CDS | 55.0% | |
| TAATAGCCCGAGAGCTTCCC+TGG | - | Chr3:56631464-56631483 | None:intergenic | 55.0% | |
| TTGATGCCAGGGAAGCTCTC+GGG | + | Chr3:56631455-56631474 | MsG0380014572.01.T01:CDS | 55.0% | |
| ! | GGTGGATTCCAAGACGGTAC+CGG | + | Chr3:56631534-56631553 | MsG0380014572.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 56631364 | 56631777 | 56631364 | ID=MsG0380014572.01;Name=MsG0380014572.01 |
| Chr3 | mRNA | 56631364 | 56631777 | 56631364 | ID=MsG0380014572.01.T01;Parent=MsG0380014572.01;Name=MsG0380014572.01.T01;_AED=0.41;_eAED=0.41;_QI=0|-1|0|1|-1|0|1|0|137 |
| Chr3 | exon | 56631364 | 56631777 | 56631364 | ID=MsG0380014572.01.T01:exon:28885;Parent=MsG0380014572.01.T01 |
| Chr3 | CDS | 56631364 | 56631777 | 56631364 | ID=MsG0380014572.01.T01:cds;Parent=MsG0380014572.01.T01 |
| Gene Sequence |
| Protein sequence |
