Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014841.01.T01 | RHN67695.1 | 79.07 | 172 | 25 | 2 | 4 | 165 | 39 | 209 | 2.91E-85 | 261 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380014841.01 | MsG0580027688.01 | 0.801440 | 8.995670e-49 | 4.296206e-46 |
| MsG0380014841.01 | MsG0580029554.01 | 0.815879 | 7.373266e-52 | 5.145054e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014841.01.T01 | MTR_3g464920 | 77.586 | 174 | 28 | 2 | 2 | 165 | 1539 | 1711 | 3.49e-83 | 267 |
| MsG0380014841.01.T01 | MTR_3g464810 | 86.000 | 150 | 20 | 1 | 16 | 165 | 1704 | 1852 | 8.48e-81 | 260 |
| MsG0380014841.01.T01 | MTR_3g464840 | 85.333 | 150 | 17 | 1 | 16 | 165 | 1540 | 1684 | 9.45e-79 | 254 |
| MsG0380014841.01.T01 | MTR_3g063010 | 82.432 | 148 | 26 | 0 | 18 | 165 | 1611 | 1758 | 3.85e-76 | 247 |
| MsG0380014841.01.T01 | MTR_3g464870 | 80.667 | 150 | 28 | 1 | 16 | 165 | 1685 | 1833 | 3.44e-75 | 244 |
| MsG0380014841.01.T01 | MTR_3g464950 | 80.795 | 151 | 28 | 1 | 15 | 165 | 1595 | 1744 | 5.85e-75 | 243 |
| MsG0380014841.01.T01 | MTR_3g062320 | 78.621 | 145 | 31 | 0 | 21 | 165 | 1425 | 1569 | 1.64e-71 | 234 |
| MsG0380014841.01.T01 | MTR_3g062980 | 70.370 | 162 | 39 | 1 | 4 | 165 | 509 | 661 | 6.99e-69 | 221 |
| MsG0380014841.01.T01 | MTR_3g062180 | 72.109 | 147 | 41 | 0 | 16 | 162 | 479 | 625 | 2.07e-65 | 211 |
| MsG0380014841.01.T01 | MTR_2g049960 | 69.655 | 145 | 44 | 0 | 21 | 165 | 135 | 279 | 3.30e-65 | 202 |
| MsG0380014841.01.T01 | MTR_3g062400 | 64.968 | 157 | 55 | 0 | 12 | 168 | 109 | 265 | 5.42e-65 | 201 |
| MsG0380014841.01.T01 | MTR_3g062140 | 72.109 | 147 | 41 | 0 | 16 | 162 | 1597 | 1743 | 1.84e-63 | 211 |
| MsG0380014841.01.T01 | MTR_3g027360 | 71.111 | 135 | 39 | 0 | 18 | 152 | 144 | 278 | 1.73e-61 | 191 |
| MsG0380014841.01.T01 | MTR_3g062340 | 70.270 | 148 | 44 | 0 | 18 | 165 | 1758 | 1905 | 4.53e-61 | 204 |
| MsG0380014841.01.T01 | MTR_3g451150 | 73.381 | 139 | 36 | 1 | 20 | 158 | 1705 | 1842 | 1.64e-59 | 199 |
| MsG0380014841.01.T01 | MTR_3g452250 | 73.381 | 139 | 36 | 1 | 20 | 158 | 1705 | 1842 | 1.64e-59 | 199 |
| MsG0380014841.01.T01 | MTR_3g466750 | 66.667 | 150 | 50 | 0 | 16 | 165 | 1675 | 1824 | 2.42e-58 | 196 |
| MsG0380014841.01.T01 | MTR_3g062420 | 68.027 | 147 | 37 | 1 | 16 | 162 | 1772 | 1908 | 4.06e-58 | 195 |
| MsG0380014841.01.T01 | MTR_0922s0020 | 64.085 | 142 | 50 | 1 | 21 | 162 | 39 | 179 | 2.07e-56 | 176 |
| MsG0380014841.01.T01 | MTR_3g435040 | 66.901 | 142 | 46 | 1 | 21 | 162 | 1667 | 1807 | 6.57e-55 | 186 |
| MsG0380014841.01.T01 | MTR_3g435040 | 66.901 | 142 | 46 | 1 | 21 | 162 | 1450 | 1590 | 6.58e-55 | 186 |
| MsG0380014841.01.T01 | MTR_3g435040 | 66.901 | 142 | 46 | 1 | 21 | 162 | 1670 | 1810 | 7.38e-55 | 186 |
| MsG0380014841.01.T01 | MTR_3g435040 | 66.901 | 142 | 46 | 1 | 21 | 162 | 1452 | 1592 | 7.68e-55 | 186 |
| MsG0380014841.01.T01 | MTR_3g435040 | 66.901 | 142 | 46 | 1 | 21 | 162 | 1391 | 1531 | 7.81e-55 | 186 |
| MsG0380014841.01.T01 | MTR_3g062010 | 64.828 | 145 | 51 | 0 | 21 | 165 | 1071 | 1215 | 2.27e-53 | 182 |
| MsG0380014841.01.T01 | MTR_7g026240 | 82.955 | 88 | 15 | 0 | 78 | 165 | 208 | 295 | 1.51e-47 | 157 |
| MsG0380014841.01.T01 | MTR_2g451270 | 57.241 | 145 | 34 | 1 | 21 | 165 | 91 | 207 | 2.67e-46 | 152 |
| MsG0380014841.01.T01 | MTR_3g069220 | 64.423 | 104 | 37 | 0 | 21 | 124 | 26 | 129 | 2.39e-39 | 131 |
| MsG0380014841.01.T01 | MTR_1147s0010 | 53.237 | 139 | 56 | 2 | 24 | 162 | 10 | 139 | 3.89e-39 | 130 |
| MsG0380014841.01.T01 | MTR_2g023000 | 53.472 | 144 | 44 | 1 | 17 | 160 | 291 | 411 | 6.24e-39 | 137 |
| MsG0380014841.01.T01 | MTR_3g062130 | 51.333 | 150 | 38 | 1 | 16 | 165 | 280 | 394 | 1.04e-37 | 134 |
| MsG0380014841.01.T01 | MTR_3g048690 | 70.213 | 94 | 28 | 0 | 21 | 114 | 1162 | 1255 | 2.03e-34 | 127 |
| MsG0380014841.01.T01 | MTR_3g466550 | 82.692 | 52 | 9 | 0 | 114 | 165 | 17 | 68 | 1.41e-23 | 89.7 |
| MsG0380014841.01.T01 | MTR_2g450490 | 87.500 | 48 | 6 | 0 | 118 | 165 | 179 | 226 | 1.68e-22 | 90.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 35 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGATGCCTTGCAGAAATTAT+TGG | 0.196050 | 3:-61018074 | MsG0380014841.01.T01:CDS |
| TCCAAGAATCATTTGGATTC+TGG | 0.217220 | 3:-61018367 | MsG0380014841.01.T01:CDS |
| GCGAGATGGGTAGGGTTATC+AGG | 0.218220 | 3:-61018581 | None:intergenic |
| TGAAAGGCGGGCTTTGTTTC+CGG | 0.241139 | 3:-61018113 | MsG0380014841.01.T01:CDS |
| TCCAGAATCCAAATGATTCT+TGG | 0.261031 | 3:+61018366 | None:intergenic |
| CCAATACTTCCAATATACTA+TGG | 0.270209 | 3:+61018215 | None:intergenic |
| CATAGTATATTGGAAGTATT+GGG | 0.292150 | 3:-61018214 | MsG0380014841.01.T01:CDS |
| CCTCAAAACCCATAGTATAT+TGG | 0.369603 | 3:-61018224 | MsG0380014841.01.T01:CDS |
| CCATAGTATATTGGAAGTAT+TGG | 0.380122 | 3:-61018215 | MsG0380014841.01.T01:CDS |
| TAGTTTAGCTAGTACGTTGT+AGG | 0.383025 | 3:+61018255 | None:intergenic |
| ATTATTGGATTCCAAGCAAC+AGG | 0.384055 | 3:-61018059 | MsG0380014841.01.T01:CDS |
| GTCATTCCACTCTGAATTTG+TGG | 0.386153 | 3:-61018191 | MsG0380014841.01.T01:CDS |
| TCTTGTGATATTTGTATATC+CGG | 0.389600 | 3:+61018094 | None:intergenic |
| ACTGGTCTCCAAGAATCATT+TGG | 0.409664 | 3:-61018374 | MsG0380014841.01.T01:CDS |
| GATGAGTGAGCTTGAGCAAC+TGG | 0.412700 | 3:-61018392 | MsG0380014841.01.T01:CDS |
| ATCCTTGGTAACCTGTTGCT+TGG | 0.437795 | 3:+61018048 | None:intergenic |
| TTACAAGATGCGCGCAGCTT+TGG | 0.443077 | 3:-61018163 | MsG0380014841.01.T01:CDS |
| TTTGATAAGGATTGGTTGGA+TGG | 0.483587 | 3:-61018139 | MsG0380014841.01.T01:CDS |
| AGGTTTCCGGCGAGATGGGT+AGG | 0.490187 | 3:-61018590 | None:intergenic |
| ACGCAACACTTCAACATCCT+TGG | 0.509653 | 3:+61018033 | None:intergenic |
| GGCTCTGTTGTATCTAGTAA+AGG | 0.510067 | 3:-61018540 | MsG0380014841.01.T01:intron |
| CAATACTTCCAATATACTAT+GGG | 0.513578 | 3:+61018216 | None:intergenic |
| TTCACTTAGTAATACATACA+AGG | 0.519506 | 3:-61018284 | MsG0380014841.01.T01:CDS |
| GTTGGATGGTGTTGAAAGGC+GGG | 0.525245 | 3:-61018125 | MsG0380014841.01.T01:CDS |
| GGTTTCCGGCGAGATGGGTA+GGG | 0.540978 | 3:-61018589 | None:intergenic |
| GGTTGGATGGTGTTGAAAGG+CGG | 0.541314 | 3:-61018126 | MsG0380014841.01.T01:CDS |
| AGGGCACAACGTAAAGGAGC+AGG | 0.552983 | 3:-61018561 | MsG0380014841.01.T01:CDS |
| CGAGATGGGTAGGGTTATCA+GGG | 0.554090 | 3:-61018580 | None:intergenic |
| GTTATCAGGGCACAACGTAA+AGG | 0.562139 | 3:-61018567 | MsG0380014841.01.T01:CDS |
| ATTGGTTGGATGGTGTTGAA+AGG | 0.563342 | 3:-61018129 | MsG0380014841.01.T01:CDS |
| GGAATCCAATAATTTCTGCA+AGG | 0.618987 | 3:+61018069 | None:intergenic |
| TCACTTAGTAATACATACAA+GGG | 0.621476 | 3:-61018283 | MsG0380014841.01.T01:CDS |
| TTCCAAGCAACAGGTTACCA+AGG | 0.650738 | 3:-61018050 | MsG0380014841.01.T01:CDS |
| ATAGTATATTGGAAGTATTG+GGG | 0.681870 | 3:-61018213 | MsG0380014841.01.T01:CDS |
| ATAACTCCACAAATTCAGAG+TGG | 0.716330 | 3:+61018185 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AGTAAAGGTATTTTATTGTT+TGG | - | Chr3:61018066-61018085 | MsG0380014841.01.T01:CDS | 20.0% |
| !!! | TAAGTTTGTTGTATTCAAAT+AGG | - | Chr3:61018103-61018122 | MsG0380014841.01.T01:CDS | 20.0% |
| ! | CAATACTTCCAATATACTAT+GGG | + | Chr3:61018378-61018397 | None:intergenic | 25.0% |
| ! | TCACTTAGTAATACATACAA+GGG | - | Chr3:61018308-61018327 | MsG0380014841.01.T01:CDS | 25.0% |
| ! | TCTTGTGATATTTGTATATC+CGG | + | Chr3:61018500-61018519 | None:intergenic | 25.0% |
| ! | TTCACTTAGTAATACATACA+AGG | - | Chr3:61018307-61018326 | MsG0380014841.01.T01:CDS | 25.0% |
| !! | GTGTCTTTTAAATAGATAGA+AGG | + | Chr3:61018287-61018306 | None:intergenic | 25.0% |
| !!! | AAATCTTCTACTTTTTGAGA+AGG | + | Chr3:61018161-61018180 | None:intergenic | 25.0% |
| !!! | AATCTTCTACTTTTTGAGAA+GGG | + | Chr3:61018160-61018179 | None:intergenic | 25.0% |
| !!! | ATAGTATATTGGAAGTATTG+GGG | - | Chr3:61018378-61018397 | MsG0380014841.01.T01:CDS | 25.0% |
| !!! | CATAGTATATTGGAAGTATT+GGG | - | Chr3:61018377-61018396 | MsG0380014841.01.T01:CDS | 25.0% |
| CCAATACTTCCAATATACTA+TGG | + | Chr3:61018379-61018398 | None:intergenic | 30.0% | |
| CTTCTTTCCTATAATTTCAG+AGG | - | Chr3:61018133-61018152 | MsG0380014841.01.T01:CDS | 30.0% | |
| ! | CCATAGTATATTGGAAGTAT+TGG | - | Chr3:61018376-61018395 | MsG0380014841.01.T01:CDS | 30.0% |
| AGATGCCTTGCAGAAATTAT+TGG | - | Chr3:61018517-61018536 | MsG0380014841.01.T01:intron | 35.0% | |
| ATAACTCCACAAATTCAGAG+TGG | + | Chr3:61018409-61018428 | None:intergenic | 35.0% | |
| ATTATTGGATTCCAAGCAAC+AGG | - | Chr3:61018532-61018551 | MsG0380014841.01.T01:intron | 35.0% | |
| CCTCAAAACCCATAGTATAT+TGG | - | Chr3:61018367-61018386 | MsG0380014841.01.T01:CDS | 35.0% | |
| GGAATCCAATAATTTCTGCA+AGG | + | Chr3:61018525-61018544 | None:intergenic | 35.0% | |
| TAGTTTAGCTAGTACGTTGT+AGG | + | Chr3:61018339-61018358 | None:intergenic | 35.0% | |
| TCCAAGAATCATTTGGATTC+TGG | - | Chr3:61018224-61018243 | MsG0380014841.01.T01:CDS | 35.0% | |
| TCCAGAATCCAAATGATTCT+TGG | + | Chr3:61018228-61018247 | None:intergenic | 35.0% | |
| ! | CCAATATACTATGGGTTTTG+AGG | + | Chr3:61018370-61018389 | None:intergenic | 35.0% |
| !! | TTTGATAAGGATTGGTTGGA+TGG | - | Chr3:61018452-61018471 | MsG0380014841.01.T01:intron | 35.0% |
| !!! | GCTTTGGTTTTGATAAGGAT+TGG | - | Chr3:61018444-61018463 | MsG0380014841.01.T01:intron | 35.0% |
| !!! | TGGTTTTGATAAGGATTGGT+TGG | - | Chr3:61018448-61018467 | MsG0380014841.01.T01:intron | 35.0% |
| GGCTCTGTTGTATCTAGTAA+AGG | - | Chr3:61018051-61018070 | MsG0380014841.01.T01:CDS | 40.0% | |
| GTCATTCCACTCTGAATTTG+TGG | - | Chr3:61018400-61018419 | MsG0380014841.01.T01:CDS | 40.0% | |
| ! | ACTGGTCTCCAAGAATCATT+TGG | - | Chr3:61018217-61018236 | MsG0380014841.01.T01:CDS | 40.0% |
| ! | ATTGGTTGGATGGTGTTGAA+AGG | - | Chr3:61018462-61018481 | MsG0380014841.01.T01:intron | 40.0% |
| !! | GAAGGGTCCTCTGAAATTAT+AGG | + | Chr3:61018143-61018162 | None:intergenic | 40.0% |
| ACGCAACACTTCAACATCCT+TGG | + | Chr3:61018561-61018580 | None:intergenic | 45.0% | |
| GTTATCAGGGCACAACGTAA+AGG | - | Chr3:61018024-61018043 | MsG0380014841.01.T01:CDS | 45.0% | |
| TTCCAAGCAACAGGTTACCA+AGG | - | Chr3:61018541-61018560 | MsG0380014841.01.T01:CDS | 45.0% | |
| ! | ATCCTTGGTAACCTGTTGCT+TGG | + | Chr3:61018546-61018565 | None:intergenic | 45.0% |
| !!! | GCGCAGCTTTGGTTTTGATA+AGG | - | Chr3:61018439-61018458 | MsG0380014841.01.T01:CDS | 45.0% |
| GATGAGTGAGCTTGAGCAAC+TGG | - | Chr3:61018199-61018218 | MsG0380014841.01.T01:CDS | 50.0% | |
| TTACAAGATGCGCGCAGCTT+TGG | - | Chr3:61018428-61018447 | MsG0380014841.01.T01:CDS | 50.0% | |
| ! | GGTTGGATGGTGTTGAAAGG+CGG | - | Chr3:61018465-61018484 | MsG0380014841.01.T01:intron | 50.0% |
| ! | GTTGGATGGTGTTGAAAGGC+GGG | - | Chr3:61018466-61018485 | MsG0380014841.01.T01:intron | 50.0% |
| !! | TGAAAGGCGGGCTTTGTTTC+CGG | - | Chr3:61018478-61018497 | MsG0380014841.01.T01:intron | 50.0% |
| AGGGCACAACGTAAAGGAGC+AGG | - | Chr3:61018030-61018049 | MsG0380014841.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 61018015 | 61018598 | 61018015 | ID=MsG0380014841.01;Name=MsG0380014841.01 |
| Chr3 | mRNA | 61018015 | 61018598 | 61018015 | ID=MsG0380014841.01.T01;Parent=MsG0380014841.01;Name=MsG0380014841.01.T01;_AED=0.55;_eAED=0.55;_QI=0|0|0|0.5|1|0.5|2|0|168 |
| Chr3 | exon | 61018541 | 61018598 | 61018541 | ID=MsG0380014841.01.T01:exon:6206;Parent=MsG0380014841.01.T01 |
| Chr3 | exon | 61018015 | 61018460 | 61018015 | ID=MsG0380014841.01.T01:exon:6205;Parent=MsG0380014841.01.T01 |
| Chr3 | CDS | 61018541 | 61018598 | 61018541 | ID=MsG0380014841.01.T01:cds;Parent=MsG0380014841.01.T01 |
| Chr3 | CDS | 61018015 | 61018460 | 61018015 | ID=MsG0380014841.01.T01:cds;Parent=MsG0380014841.01.T01 |
| Gene Sequence |
| Protein sequence |
