Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580027688.01.T01 | XP_003593377.1 | 43.003 | 393 | 139 | 10 | 94 | 444 | 67 | 416 | 1.29E-70 | 239 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580027688.01.T01 | O23063 | 34.483 | 174 | 97 | 5 | 148 | 305 | 78 | 250 | 1.20E-17 | 87.4 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580027688.01.T01 | G7IK18 | 43.003 | 393 | 139 | 10 | 94 | 444 | 67 | 416 | 6.16e-71 | 239 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048431.01 | MsG0580027688.01 | 0.801627 | 8.235872e-49 | 3.951813e-46 |
| MsG0080048874.01 | MsG0580027688.01 | 0.826326 | 2.884315e-54 | 2.696346e-51 |
| MsG0180000065.01 | MsG0580027688.01 | 0.804100 | 2.540568e-49 | 1.298602e-46 |
| MsG0180000415.01 | MsG0580027688.01 | 0.820059 | 8.379468e-53 | 6.561391e-50 |
| MsG0180000447.01 | MsG0580027688.01 | 0.820365 | 7.127662e-53 | 5.628460e-50 |
| MsG0180000470.01 | MsG0580027688.01 | 0.807926 | 3.981329e-50 | 2.245822e-47 |
| MsG0180001401.01 | MsG0580027688.01 | 0.833038 | 6.709654e-56 | 7.635406e-53 |
| MsG0180001410.01 | MsG0580027688.01 | 0.834125 | 3.590197e-56 | 4.220594e-53 |
| MsG0180002082.01 | MsG0580027688.01 | 0.813421 | 2.581979e-51 | 1.685375e-48 |
| MsG0180002089.01 | MsG0580027688.01 | 0.840806 | 6.965037e-58 | 1.003576e-54 |
| MsG0180002195.01 | MsG0580027688.01 | 0.846014 | 2.833174e-59 | 4.810436e-56 |
| MsG0180002234.01 | MsG0580027688.01 | 0.810094 | 1.367294e-50 | 8.167862e-48 |
| MsG0180002282.01 | MsG0580027688.01 | 0.803119 | 4.058419e-49 | 2.022733e-46 |
| MsG0180002518.01 | MsG0580027688.01 | 0.803184 | 3.933895e-49 | 1.964045e-46 |
| MsG0180002563.01 | MsG0580027688.01 | 0.801226 | 9.948526e-49 | 4.725787e-46 |
| MsG0180003438.01 | MsG0580027688.01 | 0.812078 | 5.079713e-51 | 3.198542e-48 |
| MsG0180004264.01 | MsG0580027688.01 | 0.805269 | 1.447975e-49 | 7.627310e-47 |
| MsG0180005438.01 | MsG0580027688.01 | 0.801090 | 1.060877e-48 | 5.022436e-46 |
| MsG0180005705.01 | MsG0580027688.01 | 0.800706 | 1.270561e-48 | 5.956247e-46 |
| MsG0180005974.01 | MsG0580027688.01 | 0.809817 | 1.568352e-50 | 9.301508e-48 |
| MsG0280011032.01 | MsG0580027688.01 | 0.820038 | 8.472423e-53 | 6.630305e-50 |
| MsG0380011759.01 | MsG0580027688.01 | 0.881368 | 2.543726e-70 | 1.486197e-66 |
| MsG0380011786.01 | MsG0580027688.01 | 0.826750 | 2.285439e-54 | 2.162458e-51 |
| MsG0380011870.01 | MsG0580027688.01 | 0.913356 | 6.769480e-84 | 1.594677e-79 |
| MsG0380011871.01 | MsG0580027688.01 | 0.821394 | 4.132279e-53 | 3.357825e-50 |
| MsG0380012702.01 | MsG0580027688.01 | 0.805027 | 1.627068e-49 | 8.517582e-47 |
| MsG0380012885.01 | MsG0580027688.01 | 0.802881 | 4.544615e-49 | 2.251474e-46 |
| MsG0380013596.01 | MsG0580027688.01 | 0.859989 | 2.823240e-63 | 7.600048e-60 |
| MsG0380014135.01 | MsG0580027688.01 | 0.808424 | 3.117727e-50 | 1.782054e-47 |
| MsG0380014142.01 | MsG0580027688.01 | 0.800652 | 1.303766e-48 | 6.103772e-46 |
| MsG0380014153.01 | MsG0580027688.01 | 0.812220 | 4.730514e-51 | 2.990303e-48 |
| MsG0380014251.01 | MsG0580027688.01 | 0.806588 | 7.648804e-50 | 4.167356e-47 |
| MsG0380014841.01 | MsG0580027688.01 | 0.801440 | 8.995670e-49 | 4.296206e-46 |
| MsG0480019730.01 | MsG0580027688.01 | 0.859542 | 3.847716e-63 | 1.019811e-59 |
| MsG0480021350.01 | MsG0580027688.01 | 0.805916 | 1.059233e-49 | 5.673326e-47 |
| MsG0480022259.01 | MsG0580027688.01 | 0.821612 | 3.681532e-53 | 3.009632e-50 |
| MsG0480022262.01 | MsG0580027688.01 | 0.806750 | 7.069395e-50 | 3.867787e-47 |
| MsG0480023248.01 | MsG0580027688.01 | 0.804079 | 2.566033e-49 | 1.310941e-46 |
| MsG0480023427.01 | MsG0580027688.01 | 0.806716 | 7.185713e-50 | 3.928030e-47 |
| MsG0480023654.01 | MsG0580027688.01 | 0.808490 | 3.019451e-50 | 1.728801e-47 |
| MsG0580024363.01 | MsG0580027688.01 | -0.801272 | 9.737058e-49 | 4.630703e-46 |
| MsG0580024621.01 | MsG0580027688.01 | 0.935931 | 3.895072e-97 | 3.226093e-92 |
| MsG0580025014.01 | MsG0580027688.01 | 0.810032 | 1.410202e-50 | 8.410941e-48 |
| MsG0580026040.01 | MsG0580027688.01 | 0.822199 | 2.691092e-53 | 2.237098e-50 |
| MsG0380014977.01 | MsG0580027688.01 | 0.823837 | 1.117325e-53 | 9.730041e-51 |
| MsG0380015821.01 | MsG0580027688.01 | 0.810084 | 1.374044e-50 | 8.205864e-48 |
| MsG0380016475.01 | MsG0580027688.01 | 0.834861 | 2.346552e-56 | 2.820163e-53 |
| MsG0380017233.01 | MsG0580027688.01 | 0.813184 | 2.911042e-51 | 1.887799e-48 |
| MsG0380017820.01 | MsG0580027688.01 | 0.841373 | 4.942791e-58 | 7.248110e-55 |
| MsG0380017821.01 | MsG0580027688.01 | 0.864652 | 1.042309e-64 | 3.297839e-61 |
| MsG0380017822.01 | MsG0580027688.01 | 0.858763 | 6.589210e-63 | 1.700320e-59 |
| MsG0380017848.01 | MsG0580027688.01 | 0.838099 | 3.517113e-57 | 4.661969e-54 |
| MsG0580026165.01 | MsG0580027688.01 | 0.816216 | 6.201164e-52 | 4.367569e-49 |
| MsG0580026222.01 | MsG0580027688.01 | 0.846755 | 1.779453e-59 | 3.094534e-56 |
| MsG0580026422.01 | MsG0580027688.01 | 0.844134 | 9.124558e-59 | 1.459181e-55 |
| MsG0580027332.01 | MsG0580027688.01 | 0.833736 | 4.492822e-56 | 5.220369e-53 |
| MsG0580027628.01 | MsG0580027688.01 | 0.800901 | 1.159664e-48 | 5.463812e-46 |
| MsG0580027687.01 | MsG0580027688.01 | 0.969248 | 7.619434e-130 | 6.856529e-124 |
| MsG0580027688.01 | MsG0580027766.01 | 0.806388 | 8.428948e-50 | 4.569023e-47 |
| MsG0580027688.01 | MsG0580027767.01 | 0.808703 | 2.718863e-50 | 1.565324e-47 |
| MsG0580027688.01 | MsG0580027926.01 | 0.833086 | 6.525205e-56 | 7.436513e-53 |
| MsG0580027688.01 | MsG0580027986.01 | 0.810664 | 1.029875e-50 | 6.245802e-48 |
| MsG0580027688.01 | MsG0580028527.01 | 0.818468 | 1.929481e-52 | 1.445600e-49 |
| MsG0580027688.01 | MsG0580028990.01 | 0.807717 | 4.409683e-50 | 2.474021e-47 |
| MsG0580027688.01 | MsG0580029204.01 | 0.853498 | 2.297357e-61 | 4.973905e-58 |
| MsG0580027688.01 | MsG0580029381.01 | 0.810654 | 1.035325e-50 | 6.276910e-48 |
| MsG0580027688.01 | MsG0580029500.01 | -0.813946 | 1.978447e-51 | 1.309881e-48 |
| MsG0580027688.01 | MsG0580029554.01 | 0.857497 | 1.567738e-62 | 3.877269e-59 |
| MsG0580027688.01 | MsG0580030168.01 | 0.824294 | 8.724675e-54 | 7.695597e-51 |
| MsG0580027688.01 | MsG0680030316.01 | 0.813428 | 2.572230e-51 | 1.679341e-48 |
| MsG0580027688.01 | MsG0680030667.01 | 0.814347 | 1.613733e-51 | 1.080143e-48 |
| MsG0580027688.01 | MsG0680030800.01 | 0.843602 | 1.267008e-58 | 1.992685e-55 |
| MsG0580027688.01 | MsG0680030886.01 | 0.810756 | 9.840420e-51 | 5.982469e-48 |
| MsG0580027688.01 | MsG0680031114.01 | 0.810152 | 1.328493e-50 | 7.948105e-48 |
| MsG0580027688.01 | MsG0680031156.01 | 0.841859 | 3.679379e-58 | 5.477965e-55 |
| MsG0580027688.01 | MsG0680031210.01 | 0.809401 | 1.927457e-50 | 1.130576e-47 |
| MsG0580027688.01 | MsG0680031896.01 | 0.816093 | 6.605050e-52 | 4.636411e-49 |
| MsG0580027688.01 | MsG0680032095.01 | 0.803625 | 3.188927e-49 | 1.610211e-46 |
| MsG0580027688.01 | MsG0680032801.01 | 0.851219 | 1.023196e-60 | 2.056445e-57 |
| MsG0580027688.01 | MsG0680032844.01 | 0.830974 | 2.170781e-55 | 2.323477e-52 |
| MsG0580027688.01 | MsG0680033187.01 | 0.823951 | 1.050668e-53 | 9.178976e-51 |
| MsG0580027688.01 | MsG0680033228.01 | 0.814519 | 1.478208e-51 | 9.940920e-49 |
| MsG0580027688.01 | MsG0680033379.01 | 0.885033 | 1.151806e-71 | 7.782202e-68 |
| MsG0580027688.01 | MsG0680033792.01 | 0.817085 | 3.958809e-52 | 2.855247e-49 |
| MsG0580027688.01 | MsG0680034058.01 | 0.805233 | 1.473912e-49 | 7.756725e-47 |
| MsG0580027688.01 | MsG0680034538.01 | 0.804745 | 1.863600e-49 | 9.686084e-47 |
| MsG0580027688.01 | MsG0680035389.01 | 0.822955 | 1.795396e-53 | 1.524992e-50 |
| MsG0580027688.01 | MsG0680035694.01 | 0.809830 | 1.558483e-50 | 9.246021e-48 |
| MsG0580027688.01 | MsG0680035828.01 | 0.833520 | 5.086249e-56 | 5.872146e-53 |
| MsG0580027688.01 | MsG0780036498.01 | 0.851232 | 1.014555e-60 | 2.039929e-57 |
| MsG0580027688.01 | MsG0780037457.01 | 0.803586 | 3.247640e-49 | 1.638316e-46 |
| MsG0580027688.01 | MsG0780039282.01 | 0.829121 | 6.144642e-55 | 6.230577e-52 |
| MsG0580027688.01 | MsG0780039880.01 | 0.833185 | 6.164937e-56 | 7.046330e-53 |
| MsG0580027688.01 | MsG0780040576.01 | -0.819192 | 1.321409e-52 | 1.009898e-49 |
| MsG0580027688.01 | MsG0780040788.01 | 0.838661 | 2.518829e-57 | 3.396797e-54 |
| MsG0580027688.01 | MsG0880041916.01 | 0.808305 | 3.306516e-50 | 1.883995e-47 |
| MsG0580027688.01 | MsG0880042078.01 | 0.802889 | 4.527456e-49 | 2.243448e-46 |
| MsG0580027688.01 | MsG0880042161.01 | 0.809905 | 1.501681e-50 | 8.926522e-48 |
| MsG0580027688.01 | MsG0880042454.01 | 0.836893 | 7.165183e-57 | 9.154427e-54 |
| MsG0580027688.01 | MsG0880042800.01 | 0.830639 | 2.622644e-55 | 2.779481e-52 |
| MsG0580027688.01 | MsG0880042966.01 | 0.800030 | 1.744861e-48 | 8.040762e-46 |
| MsG0580027688.01 | MsG0880044542.01 | 0.807527 | 4.838963e-50 | 2.701485e-47 |
| MsG0580027688.01 | MsG0880044554.01 | 0.828482 | 8.773649e-55 | 8.731950e-52 |
| MsG0580027688.01 | MsG0880044573.01 | 0.822178 | 2.722434e-53 | 2.261669e-50 |
| MsG0580027688.01 | MsG0880044644.01 | 0.831783 | 1.372618e-55 | 1.505084e-52 |
| MsG0580027688.01 | MsG0880045816.01 | 0.832558 | 8.825397e-56 | 9.899937e-53 |
| MsG0580027688.01 | MsG0880047425.01 | 0.838331 | 3.063708e-57 | 4.089898e-54 |
| MsG0280006456.01 | MsG0580027688.01 | 0.859278 | 4.619594e-63 | 1.213294e-59 |
| MsG0280007864.01 | MsG0580027688.01 | -0.806647 | 7.431516e-50 | 4.054961e-47 |
| MsG0280008759.01 | MsG0580027688.01 | 0.818738 | 1.675836e-52 | 1.264853e-49 |
| MsG0280008894.01 | MsG0580027688.01 | 0.810226 | 1.280758e-50 | 7.676796e-48 |
| MsG0280009248.01 | MsG0580027688.01 | 0.826070 | 3.318826e-54 | 3.079941e-51 |
| MsG0280009646.01 | MsG0580027688.01 | 0.841487 | 4.613889e-58 | 6.790209e-55 |
| MsG0280010443.01 | MsG0580027688.01 | 0.801547 | 8.550934e-49 | 4.094833e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0580027688.01 | MsG0280006564.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580027688.01.T01 | MTR_2g010790 | 43.003 | 393 | 139 | 10 | 94 | 444 | 67 | 416 | 1.56e-74 | 239 |
| MsG0580027688.01.T01 | MTR_4g109950 | 44.886 | 176 | 73 | 5 | 246 | 418 | 150 | 304 | 1.29e-25 | 106 |
| MsG0580027688.01.T01 | MTR_3g070120 | 31.967 | 244 | 117 | 9 | 213 | 435 | 149 | 364 | 5.63e-20 | 91.7 |
| MsG0580027688.01.T01 | MTR_4g109910 | 51.250 | 80 | 37 | 2 | 340 | 418 | 71 | 149 | 2.86e-12 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580027688.01.T01 | AT4G00390 | 34.483 | 174 | 97 | 5 | 148 | 305 | 78 | 250 | 1.22e-18 | 87.4 |
| MsG0580027688.01.T01 | AT1G61730 | 28.000 | 325 | 181 | 12 | 148 | 443 | 70 | 370 | 1.29e-18 | 87.4 |
Find 101 sgRNAs with CRISPR-Local
Find 120 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCGCAAACACCTCCATTT+TGG | 0.211865 | 5:+66160195 | None:intergenic |
| GAAGAATCTTCTTTGAATTC+AGG | 0.213006 | 5:+66161210 | None:intergenic |
| CAACTATTTCTTCGGGGTTT+TGG | 0.216835 | 5:+66161292 | None:intergenic |
| TCAGAACCAGATTCGGATTC+AGG | 0.219194 | 5:+66161012 | None:intergenic |
| CACTCGCTTTGTTCCAGATT+TGG | 0.224163 | 5:+66160888 | None:intergenic |
| GAAGAATCTTCCTCGGATTC+AGG | 0.232265 | 5:+66161102 | None:intergenic |
| AGTTGATTGGGCCTGGGTTT+GGG | 0.242415 | 5:+66160921 | None:intergenic |
| GAGGGAAGTTGTCGTTAATA+AGG | 0.263093 | 5:-66160479 | MsG0580027688.01.T01:CDS |
| CTAAAGGTTTAATTACTTCT+TGG | 0.266900 | 5:+66160126 | None:intergenic |
| TTCTACATCAACTATTTCTT+CGG | 0.266909 | 5:+66161284 | None:intergenic |
| TCAGGGTTTGCGACAGGTTT+AGG | 0.282148 | 5:+66161120 | None:intergenic |
| GAGTTGATTGGGCCTGGGTT+TGG | 0.283172 | 5:+66160920 | None:intergenic |
| TGAAGTTTGCAAGAAGGTTT+GGG | 0.296024 | 5:-66160569 | MsG0580027688.01.T01:CDS |
| GCGCCGGAGTTGATTGGGCC+TGG | 0.296982 | 5:+66160914 | None:intergenic |
| TGGGCAGCGCCGGAGTTGAT+TGG | 0.298293 | 5:+66160908 | None:intergenic |
| GTCAGATTCGGATTCGGTTT+CGG | 0.305012 | 5:+66160987 | None:intergenic |
| TCTACATCAACTATTTCTTC+GGG | 0.316467 | 5:+66161285 | None:intergenic |
| ACTCCGGCGCTGCCCAAATC+TGG | 0.324460 | 5:-66160901 | MsG0580027688.01.T01:CDS |
| TTGAAGTTTGCAAGAAGGTT+TGG | 0.331821 | 5:-66160570 | MsG0580027688.01.T01:CDS |
| CGCCGGAGTTGATTGGGCCT+GGG | 0.342671 | 5:+66160915 | None:intergenic |
| ACTCGCTTTGTTCCAGATTT+GGG | 0.350803 | 5:+66160889 | None:intergenic |
| ACCTTCAAGAAGTGTGGTTC+TGG | 0.360394 | 5:-66160424 | MsG0580027688.01.T01:CDS |
| TCAGGGTTTGAGATTGGTAT+AGG | 0.361191 | 5:+66161228 | None:intergenic |
| TAAATGCTGATGTGAAAATT+AGG | 0.361347 | 5:-66160513 | MsG0580027688.01.T01:CDS |
| GAAATGCACAGAGTTGATTA+AGG | 0.370653 | 5:-66160176 | MsG0580027688.01.T01:CDS |
| TAAAAGAGGGTTGGAATTGA+TGG | 0.372605 | 5:-66160269 | MsG0580027688.01.T01:CDS |
| TCAAACCCTGAATCCGAATC+TGG | 0.382191 | 5:-66161018 | MsG0580027688.01.T01:CDS |
| GGGCAGCGCCGGAGTTGATT+GGG | 0.396283 | 5:+66160909 | None:intergenic |
| GCAGTTAAACATCGCCAAAA+TGG | 0.398617 | 5:-66160209 | MsG0580027688.01.T01:CDS |
| GATTAAGGACCAGGCTAGAT+TGG | 0.403393 | 5:-66160161 | MsG0580027688.01.T01:CDS |
| AAGAATCTTCTTTGAATTCA+GGG | 0.411293 | 5:+66161211 | None:intergenic |
| GGCTTTGAATCGAGGGGTTG+TGG | 0.412020 | 5:+66160952 | None:intergenic |
| CAAGAAGGTTTGGGGTGAGA+AGG | 0.420820 | 5:-66160560 | MsG0580027688.01.T01:CDS |
| GCAGATAAAGTCAAATTGAT+TGG | 0.421576 | 5:-66160838 | MsG0580027688.01.T01:CDS |
| TTGAATTCAGGGTTTGAGAT+TGG | 0.422967 | 5:+66161222 | None:intergenic |
| ACCAGAACCACACTTCTTGA+AGG | 0.425626 | 5:+66160423 | None:intergenic |
| ACAATGGTTGAAGAAGAATA+TGG | 0.429857 | 5:-66161453 | MsG0580027688.01.T01:CDS |
| GTCAGATAGGGTTGCATTGG+TGG | 0.431334 | 5:-66160242 | MsG0580027688.01.T01:CDS |
| AATTGATGGCCGAGTCAGAT+AGG | 0.446476 | 5:-66160255 | MsG0580027688.01.T01:CDS |
| GTCGTTAATAAGGAGAAAGT+TGG | 0.449221 | 5:-66160469 | MsG0580027688.01.T01:CDS |
| GAGTAAAGTTGTCACTGGTT+AGG | 0.451669 | 5:+66160614 | None:intergenic |
| TTCGGATTCAGAACCAGATT+CGG | 0.456274 | 5:+66161005 | None:intergenic |
| CAGAACCAGATTCGGATTCA+GGG | 0.457611 | 5:+66161013 | None:intergenic |
| GAATCGAGGGGTTGTGGAGT+GGG | 0.461518 | 5:+66160958 | None:intergenic |
| AGTTGGTAAGTGTGTTAAGA+AGG | 0.464134 | 5:-66160452 | MsG0580027688.01.T01:CDS |
| TCGGATTCAGGGTTTGCGAC+AGG | 0.466200 | 5:+66161114 | None:intergenic |
| TTCGGATGAAGAATCTTCCT+CGG | 0.474524 | 5:+66161095 | None:intergenic |
| AAGAATCTTCCTCGGATTCA+GGG | 0.475948 | 5:+66161103 | None:intergenic |
| AGTGGGTTCAGCGTCAGATT+CGG | 0.478522 | 5:+66160975 | None:intergenic |
| TTCAGCGTCAGATTCGGATT+CGG | 0.479031 | 5:+66160981 | None:intergenic |
| TCGGATTCAGGGTTTGAGAC+AGG | 0.479429 | 5:+66161024 | None:intergenic |
| GACACAATCACAATTCTCAA+TGG | 0.481594 | 5:-66160778 | MsG0580027688.01.T01:CDS |
| GTTCCAGATTTGGGCAGCGC+CGG | 0.482085 | 5:+66160898 | None:intergenic |
| TTGGGGTGAGAAGGGTTCAA+AGG | 0.485409 | 5:-66160551 | MsG0580027688.01.T01:CDS |
| CACAGAGTTGATTAAGGACC+AGG | 0.492931 | 5:-66160170 | MsG0580027688.01.T01:CDS |
| TTGGAAGGAGTATGTTGAGT+TGG | 0.496367 | 5:+66161168 | None:intergenic |
| GTTAGCTAAGGTTACCAAGA+AGG | 0.497922 | 5:-66160395 | MsG0580027688.01.T01:CDS |
| TGAGTGGAAAAGTGGAAGGT+GGG | 0.499571 | 5:-66160372 | MsG0580027688.01.T01:CDS |
| GGTTGAGTTTGATGATCGTA+TGG | 0.503747 | 5:-66160320 | MsG0580027688.01.T01:CDS |
| ACACAATCACAATTCTCAAT+GGG | 0.508611 | 5:-66160777 | MsG0580027688.01.T01:CDS |
| AAGCTCTTCATCTGATGAAG+AGG | 0.514629 | 5:-66161403 | MsG0580027688.01.T01:CDS |
| AATTAGGAAGCGTGTTAAGA+GGG | 0.515847 | 5:-66160497 | MsG0580027688.01.T01:CDS |
| AGTTAGTTCTTCGATTGAGA+AGG | 0.520759 | 5:+66161323 | None:intergenic |
| TCAGGGTTTGAGACAGGTGT+AGG | 0.521232 | 5:+66161030 | None:intergenic |
| CGAGTCAGATAGGGTTGCAT+TGG | 0.531040 | 5:-66160245 | MsG0580027688.01.T01:CDS |
| AAGGAATCTGATTCGTTGGA+AGG | 0.533311 | 5:+66161153 | None:intergenic |
| CAAGAAGGTGAGTGGAAAAG+TGG | 0.535173 | 5:-66160380 | MsG0580027688.01.T01:CDS |
| TGAATCGAGGGGTTGTGGAG+TGG | 0.537206 | 5:+66160957 | None:intergenic |
| AATTGGAGTAAAGTTGTCAC+TGG | 0.542694 | 5:+66160609 | None:intergenic |
| CCAGGCTAGATTGGTACTGC+AGG | 0.542882 | 5:-66160152 | MsG0580027688.01.T01:CDS |
| GGGTTGCATTGGTGGAGAGG+TGG | 0.543166 | 5:-66160234 | MsG0580027688.01.T01:CDS |
| AAGAAGGTTTGGGGTGAGAA+GGG | 0.544850 | 5:-66160559 | MsG0580027688.01.T01:CDS |
| AAATTAGGAAGCGTGTTAAG+AGG | 0.551859 | 5:-66160498 | MsG0580027688.01.T01:CDS |
| TAGTTCTTCGATTGAGAAGG+AGG | 0.553733 | 5:+66161326 | None:intergenic |
| GTGAGTGGAAAAGTGGAAGG+TGG | 0.558206 | 5:-66160373 | MsG0580027688.01.T01:CDS |
| GCAGATTTATCAAAGAAAGA+TGG | 0.558558 | 5:-66161258 | MsG0580027688.01.T01:CDS |
| AACCCAGGCCCAATCAACTC+CGG | 0.567535 | 5:-66160917 | MsG0580027688.01.T01:CDS |
| TTCTGGTAAGAAGTTAGCTA+AGG | 0.571748 | 5:-66160407 | MsG0580027688.01.T01:CDS |
| CCTGCAGTACCAATCTAGCC+TGG | 0.574049 | 5:+66160152 | None:intergenic |
| AAGGTGAGTGGAAAAGTGGA+AGG | 0.574162 | 5:-66160376 | MsG0580027688.01.T01:CDS |
| ATAGGGTTGCATTGGTGGAG+AGG | 0.576458 | 5:-66160237 | MsG0580027688.01.T01:CDS |
| GTTCTTCGATTGAGAAGGAG+GGG | 0.577243 | 5:+66161328 | None:intergenic |
| ATTGATGGCCGAGTCAGATA+GGG | 0.577895 | 5:-66160254 | MsG0580027688.01.T01:CDS |
| TTCTTCGATTGAGAAGGAGG+GGG | 0.580865 | 5:+66161329 | None:intergenic |
| GCCCACAAAAGCCCAAACCC+AGG | 0.592097 | 5:-66160932 | MsG0580027688.01.T01:CDS |
| AGTTCTTCGATTGAGAAGGA+GGG | 0.597577 | 5:+66161327 | None:intergenic |
| CTACATCAACTATTTCTTCG+GGG | 0.599443 | 5:+66161286 | None:intergenic |
| GGGAACGGCTGCACTGAGCA+AGG | 0.601978 | 5:-66160299 | MsG0580027688.01.T01:CDS |
| CAATGCAACCCTATCTGACT+CGG | 0.612044 | 5:+66160246 | None:intergenic |
| TGTCGCAAACCCTGAATCCG+AGG | 0.615215 | 5:-66161112 | MsG0580027688.01.T01:CDS |
| AAGGTTACCAAGAAGGTGAG+TGG | 0.615671 | 5:-66160388 | MsG0580027688.01.T01:CDS |
| GAAACTACCTTCAAGAAGTG+TGG | 0.627371 | 5:-66160430 | MsG0580027688.01.T01:CDS |
| GTTAAACATCGCCAAAATGG+AGG | 0.641095 | 5:-66160206 | MsG0580027688.01.T01:CDS |
| GTTTGATGATCGTATGGGAA+CGG | 0.641811 | 5:-66160314 | MsG0580027688.01.T01:CDS |
| GAGTGGAAAAGTGGAAGGTG+GGG | 0.645972 | 5:-66160371 | MsG0580027688.01.T01:CDS |
| GAAGTTTGCAAGAAGGTTTG+GGG | 0.649228 | 5:-66160568 | MsG0580027688.01.T01:CDS |
| GTTGAGTTTGATGATCGTAT+GGG | 0.652781 | 5:-66160319 | MsG0580027688.01.T01:CDS |
| GGTTAGGTACTTATTCCTCA+CGG | 0.655887 | 5:+66160630 | None:intergenic |
| TCTTCGATTGAGAAGGAGGG+GGG | 0.657905 | 5:+66161330 | None:intergenic |
| TTTGTGGGCTTTGAATCGAG+GGG | 0.675767 | 5:+66160946 | None:intergenic |
| GATGAGTGCTGATGTTACAA+CGG | 0.745376 | 5:-66160677 | MsG0580027688.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAATTTTCTTATTACGAAT+TGG | + | Chr5:66160999-66161018 | None:intergenic | 15.0% |
| ! | AAGAATCTTCTTTGAATTCA+GGG | + | Chr5:66160380-66160399 | None:intergenic | 25.0% |
| ! | TAAATGCTGATGTGAAAATT+AGG | - | Chr5:66161075-66161094 | MsG0580027688.01.T01:CDS | 25.0% |
| !! | TAAAAAAGAATCTGATTCGT+TGG | + | Chr5:66160532-66160551 | None:intergenic | 25.0% |
| !! | TTCTACATCAACTATTTCTT+CGG | + | Chr5:66160307-66160326 | None:intergenic | 25.0% |
| !!! | AATTTTTGAAGTTTGCAAGA+AGG | - | Chr5:66161013-66161032 | MsG0580027688.01.T01:CDS | 25.0% |
| !!! | AGGTTTAGGTTCTTTTAAAA+AGG | + | Chr5:66160457-66160476 | None:intergenic | 25.0% |
| ACACAATCACAATTCTCAAT+GGG | - | Chr5:66160811-66160830 | MsG0580027688.01.T01:CDS | 30.0% | |
| GAAGAATCTTCTTTGAATTC+AGG | + | Chr5:66160381-66160400 | None:intergenic | 30.0% | |
| GCAGATAAAGTCAAATTGAT+TGG | - | Chr5:66160750-66160769 | MsG0580027688.01.T01:CDS | 30.0% | |
| GCAGATTTATCAAAGAAAGA+TGG | - | Chr5:66160330-66160349 | MsG0580027688.01.T01:CDS | 30.0% | |
| ! | ACGAGTTTCACATTTTTATG+CGG | - | Chr5:66160883-66160902 | MsG0580027688.01.T01:CDS | 30.0% |
| ! | GATACGAGTATGATTTTAAG+CGG | - | Chr5:66161242-66161261 | MsG0580027688.01.T01:CDS | 30.0% |
| ! | GTTGGAATTTTCTTCTTCTT+CGG | + | Chr5:66160514-66160533 | None:intergenic | 30.0% |
| ! | TAAAAAGGAATCTGATTCGT+TGG | + | Chr5:66160442-66160461 | None:intergenic | 30.0% |
| ! | TCTACATCAACTATTTCTTC+GGG | + | Chr5:66160306-66160325 | None:intergenic | 30.0% |
| AATTGGAGTAAAGTTGTCAC+TGG | + | Chr5:66160982-66161001 | None:intergenic | 35.0% | |
| GACACAATCACAATTCTCAA+TGG | - | Chr5:66160810-66160829 | MsG0580027688.01.T01:CDS | 35.0% | |
| GTCGTTAATAAGGAGAAAGT+TGG | - | Chr5:66161119-66161138 | MsG0580027688.01.T01:CDS | 35.0% | |
| TAAAAGAGGGTTGGAATTGA+TGG | - | Chr5:66161319-66161338 | MsG0580027688.01.T01:CDS | 35.0% | |
| TGAAGTTTGCAAGAAGGTTT+GGG | - | Chr5:66161019-66161038 | MsG0580027688.01.T01:CDS | 35.0% | |
| TTCTGGTAAGAAGTTAGCTA+AGG | - | Chr5:66161181-66161200 | MsG0580027688.01.T01:CDS | 35.0% | |
| TTGAAGTTTGCAAGAAGGTT+TGG | - | Chr5:66161018-66161037 | MsG0580027688.01.T01:CDS | 35.0% | |
| ! | AAATTAGGAAGCGTGTTAAG+AGG | - | Chr5:66161090-66161109 | MsG0580027688.01.T01:CDS | 35.0% |
| ! | AATTAGGAAGCGTGTTAAGA+GGG | - | Chr5:66161091-66161110 | MsG0580027688.01.T01:CDS | 35.0% |
| ! | AGCAAGGATTTTGCTAAAAG+AGG | - | Chr5:66161305-66161324 | MsG0580027688.01.T01:CDS | 35.0% |
| ! | AGTTAGTTCTTCGATTGAGA+AGG | + | Chr5:66160268-66160287 | None:intergenic | 35.0% |
| ! | CTACATCAACTATTTCTTCG+GGG | + | Chr5:66160305-66160324 | None:intergenic | 35.0% |
| ! | CTTGATTTTGCTGAAGAGAA+GGG | - | Chr5:66160837-66160856 | MsG0580027688.01.T01:CDS | 35.0% |
| ! | GAAATGCACAGAGTTGATTA+AGG | - | Chr5:66161412-66161431 | MsG0580027688.01.T01:CDS | 35.0% |
| ! | GAGTATGATTTTAAGCGGAA+TGG | - | Chr5:66161247-66161266 | MsG0580027688.01.T01:CDS | 35.0% |
| ! | GCAAGGATTTTGCTAAAAGA+GGG | - | Chr5:66161306-66161325 | MsG0580027688.01.T01:CDS | 35.0% |
| ! | GTTGAGTTTGATGATCGTAT+GGG | - | Chr5:66161269-66161288 | MsG0580027688.01.T01:CDS | 35.0% |
| ! | TCTTGATTTTGCTGAAGAGA+AGG | - | Chr5:66160836-66160855 | MsG0580027688.01.T01:CDS | 35.0% |
| ! | TTGAATTCAGGGTTTGAGAT+TGG | + | Chr5:66160369-66160388 | None:intergenic | 35.0% |
| !! | AGTTGGTAAGTGTGTTAAGA+AGG | - | Chr5:66161136-66161155 | MsG0580027688.01.T01:CDS | 35.0% |
| AAGAATCTTCCTCGGATTCA+GGG | + | Chr5:66160488-66160507 | None:intergenic | 40.0% | |
| AAGCTCTTCATCTGATGAAG+AGG | - | Chr5:66160185-66160204 | MsG0580027688.01.T01:CDS | 40.0% | |
| AGTTCTTCGATTGAGAAGGA+GGG | + | Chr5:66160264-66160283 | None:intergenic | 40.0% | |
| GAAACTACCTTCAAGAAGTG+TGG | - | Chr5:66161158-66161177 | MsG0580027688.01.T01:CDS | 40.0% | |
| GAAGTTTGCAAGAAGGTTTG+GGG | - | Chr5:66161020-66161039 | MsG0580027688.01.T01:CDS | 40.0% | |
| GAGGGAAGTTGTCGTTAATA+AGG | - | Chr5:66161109-66161128 | MsG0580027688.01.T01:CDS | 40.0% | |
| GCAGTTAAACATCGCCAAAA+TGG | - | Chr5:66161379-66161398 | MsG0580027688.01.T01:CDS | 40.0% | |
| GGTTAGGTACTTATTCCTCA+CGG | + | Chr5:66160961-66160980 | None:intergenic | 40.0% | |
| GTTAAACATCGCCAAAATGG+AGG | - | Chr5:66161382-66161401 | MsG0580027688.01.T01:CDS | 40.0% | |
| GTTAGCTAAGGTTACCAAGA+AGG | - | Chr5:66161193-66161212 | MsG0580027688.01.T01:CDS | 40.0% | |
| TAGTTCTTCGATTGAGAAGG+AGG | + | Chr5:66160265-66160284 | None:intergenic | 40.0% | |
| TTCGGATGAAGAATCTTCCT+CGG | + | Chr5:66160496-66160515 | None:intergenic | 40.0% | |
| TTCGGATTCAGAACCAGATT+CGG | + | Chr5:66160586-66160605 | None:intergenic | 40.0% | |
| ! | AAGGAATCTGATTCGTTGGA+AGG | + | Chr5:66160438-66160457 | None:intergenic | 40.0% |
| ! | ACTCGCTTTGTTCCAGATTT+GGG | + | Chr5:66160702-66160721 | None:intergenic | 40.0% |
| ! | GAGTAAAGTTGTCACTGGTT+AGG | + | Chr5:66160977-66160996 | None:intergenic | 40.0% |
| ! | GGATTTTGCTAAAAGAGGGT+TGG | - | Chr5:66161310-66161329 | MsG0580027688.01.T01:CDS | 40.0% |
| ! | GGTTGAGTTTGATGATCGTA+TGG | - | Chr5:66161268-66161287 | MsG0580027688.01.T01:CDS | 40.0% |
| ! | GTTTGATGATCGTATGGGAA+CGG | - | Chr5:66161274-66161293 | MsG0580027688.01.T01:CDS | 40.0% |
| ! | TCAGGGTTTGAGATTGGTAT+AGG | + | Chr5:66160363-66160382 | None:intergenic | 40.0% |
| ! | TTGGAAGGAGTATGTTGAGT+TGG | + | Chr5:66160423-66160442 | None:intergenic | 40.0% |
| ! | TTTCGCAAACACCTCCATTT+TGG | + | Chr5:66161396-66161415 | None:intergenic | 40.0% |
| ! | TTTTGCTGAAGAGAAGGGAA+AGG | - | Chr5:66160842-66160861 | MsG0580027688.01.T01:CDS | 40.0% |
| !! | CAACTATTTCTTCGGGGTTT+TGG | + | Chr5:66160299-66160318 | None:intergenic | 40.0% |
| !! | GATGAGTGCTGATGTTACAA+CGG | - | Chr5:66160911-66160930 | MsG0580027688.01.T01:CDS | 40.0% |
| !!! | TTTTGTGGGCTTTGAATCGA+GGG | + | Chr5:66160646-66160665 | None:intergenic | 40.0% |
| AAGGTGAGTGGAAAAGTGGA+AGG | - | Chr5:66161212-66161231 | MsG0580027688.01.T01:CDS | 45.0% | |
| AAGGTTACCAAGAAGGTGAG+TGG | - | Chr5:66161200-66161219 | MsG0580027688.01.T01:CDS | 45.0% | |
| ACCAGAACCACACTTCTTGA+AGG | + | Chr5:66161168-66161187 | None:intergenic | 45.0% | |
| AGCAAAAAGTTCGTTCCGTG+AGG | - | Chr5:66160943-66160962 | MsG0580027688.01.T01:CDS | 45.0% | |
| CAAGAAGGTGAGTGGAAAAG+TGG | - | Chr5:66161208-66161227 | MsG0580027688.01.T01:CDS | 45.0% | |
| CAATGCAACCCTATCTGACT+CGG | + | Chr5:66161345-66161364 | None:intergenic | 45.0% | |
| CAGAACCAGATTCGGATTCA+GGG | + | Chr5:66160578-66160597 | None:intergenic | 45.0% | |
| GAAGAATCTTCCTCGGATTC+AGG | + | Chr5:66160489-66160508 | None:intergenic | 45.0% | |
| GATTAAGGACCAGGCTAGAT+TGG | - | Chr5:66161427-66161446 | MsG0580027688.01.T01:CDS | 45.0% | |
| GTTCTTCGATTGAGAAGGAG+GGG | + | Chr5:66160263-66160282 | None:intergenic | 45.0% | |
| TCAAACCCTGAATCCGAATC+TGG | - | Chr5:66160570-66160589 | MsG0580027688.01.T01:CDS | 45.0% | |
| TCAGAACCAGATTCGGATTC+AGG | + | Chr5:66160579-66160598 | None:intergenic | 45.0% | |
| TGAGTGGAAAAGTGGAAGGT+GGG | - | Chr5:66161216-66161235 | MsG0580027688.01.T01:CDS | 45.0% | |
| TTCAGCGTCAGATTCGGATT+CGG | + | Chr5:66160610-66160629 | None:intergenic | 45.0% | |
| TTCTTCGATTGAGAAGGAGG+GGG | + | Chr5:66160262-66160281 | None:intergenic | 45.0% | |
| ! | AAGAAGGTTTGGGGTGAGAA+GGG | - | Chr5:66161029-66161048 | MsG0580027688.01.T01:CDS | 45.0% |
| ! | AATTGATGGCCGAGTCAGAT+AGG | - | Chr5:66161333-66161352 | MsG0580027688.01.T01:CDS | 45.0% |
| ! | ACCTTCAAGAAGTGTGGTTC+TGG | - | Chr5:66161164-66161183 | MsG0580027688.01.T01:CDS | 45.0% |
| ! | ATTGATGGCCGAGTCAGATA+GGG | - | Chr5:66161334-66161353 | MsG0580027688.01.T01:CDS | 45.0% |
| ! | CACAGAGTTGATTAAGGACC+AGG | - | Chr5:66161418-66161437 | MsG0580027688.01.T01:CDS | 45.0% |
| ! | CACTCGCTTTGTTCCAGATT+TGG | + | Chr5:66160703-66160722 | None:intergenic | 45.0% |
| ! | CACTTTTCCACTCACCTTCT+TGG | + | Chr5:66161210-66161229 | None:intergenic | 45.0% |
| ! | GTCAGATTCGGATTCGGTTT+CGG | + | Chr5:66160604-66160623 | None:intergenic | 45.0% |
| !! | TTTGTGGGCTTTGAATCGAG+GGG | + | Chr5:66160645-66160664 | None:intergenic | 45.0% |
| !!! | CTTTTGTGGGCTTTGAATCG+AGG | + | Chr5:66160647-66160666 | None:intergenic | 45.0% |
| AGTTGATTGGGCCTGGGTTT+GGG | + | Chr5:66160670-66160689 | None:intergenic | 50.0% | |
| CGAGTCAGATAGGGTTGCAT+TGG | - | Chr5:66161343-66161362 | MsG0580027688.01.T01:CDS | 50.0% | |
| GAGTGGAAAAGTGGAAGGTG+GGG | - | Chr5:66161217-66161236 | MsG0580027688.01.T01:CDS | 50.0% | |
| GTCAGATAGGGTTGCATTGG+TGG | - | Chr5:66161346-66161365 | MsG0580027688.01.T01:CDS | 50.0% | |
| GTGAGTGGAAAAGTGGAAGG+TGG | - | Chr5:66161215-66161234 | MsG0580027688.01.T01:CDS | 50.0% | |
| TCAGGGTTTGCGACAGGTTT+AGG | + | Chr5:66160471-66160490 | None:intergenic | 50.0% | |
| TCTTCGATTGAGAAGGAGGG+GGG | + | Chr5:66160261-66160280 | None:intergenic | 50.0% | |
| TTGGGGTGAGAAGGGTTCAA+AGG | - | Chr5:66161037-66161056 | MsG0580027688.01.T01:CDS | 50.0% | |
| ! | AGTGGGTTCAGCGTCAGATT+CGG | + | Chr5:66160616-66160635 | None:intergenic | 50.0% |
| ! | ATAGGGTTGCATTGGTGGAG+AGG | - | Chr5:66161351-66161370 | MsG0580027688.01.T01:CDS | 50.0% |
| ! | CAAGAAGGTTTGGGGTGAGA+AGG | - | Chr5:66161028-66161047 | MsG0580027688.01.T01:CDS | 50.0% |
| ! | TCAGGGTTTGAGACAGGTGT+AGG | + | Chr5:66160561-66160580 | None:intergenic | 50.0% |
| ! | TCGGATTCAGGGTTTGAGAC+AGG | + | Chr5:66160567-66160586 | None:intergenic | 50.0% |
| AACCCAGGCCCAATCAACTC+CGG | - | Chr5:66160671-66160690 | MsG0580027688.01.T01:CDS | 55.0% | |
| CCAGGCTAGATTGGTACTGC+AGG | - | Chr5:66161436-66161455 | MsG0580027688.01.T01:CDS | 55.0% | |
| CCTGCAGTACCAATCTAGCC+TGG | + | Chr5:66161439-66161458 | None:intergenic | 55.0% | |
| GAATCGAGGGGTTGTGGAGT+GGG | + | Chr5:66160633-66160652 | None:intergenic | 55.0% | |
| TCGGATTCAGGGTTTGCGAC+AGG | + | Chr5:66160477-66160496 | None:intergenic | 55.0% | |
| TGAATCGAGGGGTTGTGGAG+TGG | + | Chr5:66160634-66160653 | None:intergenic | 55.0% | |
| TGTCGCAAACCCTGAATCCG+AGG | - | Chr5:66160476-66160495 | MsG0580027688.01.T01:CDS | 55.0% | |
| ! | GAGTTGATTGGGCCTGGGTT+TGG | + | Chr5:66160671-66160690 | None:intergenic | 55.0% |
| ! | GCCTGGGTTTGGGCTTTTGT+GGG | + | Chr5:66160660-66160679 | None:intergenic | 55.0% |
| !! | GGCTTTGAATCGAGGGGTTG+TGG | + | Chr5:66160639-66160658 | None:intergenic | 55.0% |
| GCCCACAAAAGCCCAAACCC+AGG | - | Chr5:66160656-66160675 | MsG0580027688.01.T01:CDS | 60.0% | |
| GTTCCAGATTTGGGCAGCGC+CGG | + | Chr5:66160693-66160712 | None:intergenic | 60.0% | |
| ! | GGCCTGGGTTTGGGCTTTTG+TGG | + | Chr5:66160661-66160680 | None:intergenic | 60.0% |
| ! | GGGTTGCATTGGTGGAGAGG+TGG | - | Chr5:66161354-66161373 | MsG0580027688.01.T01:CDS | 60.0% |
| ACTCCGGCGCTGCCCAAATC+TGG | - | Chr5:66160687-66160706 | MsG0580027688.01.T01:CDS | 65.0% | |
| GGGAACGGCTGCACTGAGCA+AGG | - | Chr5:66161289-66161308 | MsG0580027688.01.T01:CDS | 65.0% | |
| ! | CGCCGGAGTTGATTGGGCCT+GGG | + | Chr5:66160676-66160695 | None:intergenic | 65.0% |
| ! | GGGCAGCGCCGGAGTTGATT+GGG | + | Chr5:66160682-66160701 | None:intergenic | 65.0% |
| ! | TGGGCAGCGCCGGAGTTGAT+TGG | + | Chr5:66160683-66160702 | None:intergenic | 65.0% |
| ! | GCGCCGGAGTTGATTGGGCC+TGG | + | Chr5:66160677-66160696 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 66160138 | 66161472 | 66160138 | ID=MsG0580027688.01;Name=MsG0580027688.01 |
| Chr5 | mRNA | 66160138 | 66161472 | 66160138 | ID=MsG0580027688.01.T01;Parent=MsG0580027688.01;Name=MsG0580027688.01.T01;_AED=0.55;_eAED=0.55;_QI=0|-1|0|1|-1|1|1|0|444 |
| Chr5 | exon | 66160138 | 66161472 | 66160138 | ID=MsG0580027688.01.T01:exon:34059;Parent=MsG0580027688.01.T01 |
| Chr5 | CDS | 66160138 | 66161472 | 66160138 | ID=MsG0580027688.01.T01:cds;Parent=MsG0580027688.01.T01 |
| Gene Sequence |
| Protein sequence |
