Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014990.01.T01 | RHN67881.1 | 94.915 | 59 | 3 | 0 | 1 | 59 | 154 | 212 | 1.89E-30 | 115 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014990.01.T01 | Q6J540 | 59.649 | 57 | 23 | 0 | 2 | 58 | 475 | 531 | 2.35E-16 | 73.9 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014990.01.T01 | A0A072UZE3 | 94.915 | 59 | 3 | 0 | 1 | 59 | 470 | 528 | 1.51e-28 | 115 |
| MsG0380014990.01.T01 | A0A396IT51 | 94.915 | 59 | 3 | 0 | 1 | 59 | 154 | 212 | 9.03e-31 | 115 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380014988.01 | MsG0380014990.01 | 0.805358 | 1.387578e-49 | 7.326016e-47 |
| MsG0380014989.01 | MsG0380014990.01 | 0.869753 | 2.445329e-66 | 9.278298e-63 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014990.01.T01 | MTR_3g467220 | 94.915 | 59 | 3 | 0 | 1 | 59 | 470 | 528 | 3.83e-32 | 115 |
| MsG0380014990.01.T01 | MTR_3g467200 | 76.271 | 59 | 14 | 0 | 1 | 59 | 475 | 533 | 1.72e-24 | 94.4 |
| MsG0380014990.01.T01 | MTR_3g465300 | 60.656 | 61 | 24 | 0 | 2 | 62 | 471 | 531 | 9.97e-18 | 75.1 |
| MsG0380014990.01.T01 | MTR_8g073960 | 52.632 | 57 | 27 | 0 | 1 | 57 | 472 | 528 | 2.52e-15 | 68.2 |
| MsG0380014990.01.T01 | MTR_8g076372 | 50.877 | 57 | 28 | 0 | 1 | 57 | 459 | 515 | 1.96e-14 | 65.9 |
| MsG0380014990.01.T01 | MTR_8g076390 | 50.877 | 57 | 28 | 0 | 1 | 57 | 471 | 527 | 1.96e-14 | 65.9 |
| MsG0380014990.01.T01 | MTR_3g467290 | 55.932 | 59 | 25 | 1 | 1 | 58 | 480 | 538 | 7.84e-14 | 63.9 |
| MsG0380014990.01.T01 | MTR_8g073950 | 47.368 | 57 | 30 | 0 | 1 | 57 | 468 | 524 | 3.93e-13 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014990.01.T01 | AT5G35920 | 46.429 | 56 | 30 | 0 | 2 | 57 | 98 | 153 | 9.76e-11 | 53.9 |
Find 11 sgRNAs with CRISPR-Local
Find 11 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATATTGATTCTTGATATAT+TGG | 0.177519 | 3:-63671952 | None:intergenic |
| GGCTTTACTGCAACCGTTAG+TGG | 0.355500 | 3:-63672008 | None:intergenic |
| ATATTGATTCTTGATATATT+GGG | 0.375181 | 3:-63671951 | None:intergenic |
| GGTTACTCCATGGCTTCACT+TGG | 0.446859 | 3:+63671919 | MsG0380014990.01.T01:CDS |
| GGTTGTCCTGGTATCATGCT+TGG | 0.499438 | 3:+63671867 | None:intergenic |
| TACAATTCTGCTGCTAATCG+TGG | 0.533354 | 3:-63672029 | None:intergenic |
| ATCTCTTGATGAGCCACTAA+CGG | 0.553205 | 3:+63671995 | MsG0380014990.01.T01:CDS |
| TGACTATAATGCTATTAGCT+AGG | 0.558207 | 3:+63671898 | MsG0380014990.01.T01:CDS |
| GTGCACTCCAAGTGAAGCCA+TGG | 0.562249 | 3:-63671926 | None:intergenic |
| CTATTAGCTAGGTTACTCCA+TGG | 0.577297 | 3:+63671909 | MsG0380014990.01.T01:CDS |
| TAATAGCATTATAGTCATTG+TGG | 0.627153 | 3:-63671892 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATATTGATTCTTGATATAT+TGG | - | Chr3:63671955-63671974 | None:intergenic | 15.0% |
| !!! | ATATTGATTCTTGATATATT+GGG | - | Chr3:63671954-63671973 | None:intergenic | 15.0% |
| ! | TAATAGCATTATAGTCATTG+TGG | - | Chr3:63671895-63671914 | None:intergenic | 25.0% |
| !!! | ATCAATATTTTCGATTCGAA+TGG | + | Chr3:63671966-63671985 | MsG0380014990.01.T01:CDS | 25.0% |
| TGACTATAATGCTATTAGCT+AGG | + | Chr3:63671898-63671917 | MsG0380014990.01.T01:CDS | 30.0% | |
| ATCTCTTGATGAGCCACTAA+CGG | + | Chr3:63671995-63672014 | MsG0380014990.01.T01:CDS | 40.0% | |
| CTATTAGCTAGGTTACTCCA+TGG | + | Chr3:63671909-63671928 | MsG0380014990.01.T01:CDS | 40.0% | |
| TACAATTCTGCTGCTAATCG+TGG | - | Chr3:63672032-63672051 | None:intergenic | 40.0% | |
| GGCTTTACTGCAACCGTTAG+TGG | - | Chr3:63672011-63672030 | None:intergenic | 50.0% | |
| GGTTACTCCATGGCTTCACT+TGG | + | Chr3:63671919-63671938 | MsG0380014990.01.T01:CDS | 50.0% | |
| GTGCACTCCAAGTGAAGCCA+TGG | - | Chr3:63671929-63671948 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 63671882 | 63672070 | 63671882 | ID=MsG0380014990.01;Name=MsG0380014990.01 |
| Chr3 | mRNA | 63671882 | 63672070 | 63671882 | ID=MsG0380014990.01.T01;Parent=MsG0380014990.01;Name=MsG0380014990.01.T01;_AED=0.40;_eAED=0.40;_QI=0|-1|0|1|-1|0|1|0|62 |
| Chr3 | exon | 63671882 | 63672070 | 63671882 | ID=MsG0380014990.01.T01:exon:29782;Parent=MsG0380014990.01.T01 |
| Chr3 | CDS | 63671882 | 63672070 | 63671882 | ID=MsG0380014990.01.T01:cds;Parent=MsG0380014990.01.T01 |
| Gene Sequence |
| Protein sequence |
