Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015195.01.T01 | XP_013444917.1 | 89.071 | 183 | 20 | 0 | 1 | 183 | 1 | 183 | 3.52E-119 | 345 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015195.01.T01 | A0A072TNG9 | 89.071 | 183 | 20 | 0 | 1 | 183 | 1 | 183 | 1.68e-119 | 345 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380015195.01 | MsG0880043288.01 | 0.809002 | 2.346534e-50 | 1.361702e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015195.01.T01 | MTR_8g432460 | 89.071 | 183 | 20 | 0 | 1 | 183 | 1 | 183 | 4.27e-123 | 345 |
| MsG0380015195.01.T01 | MTR_8g432490 | 89.617 | 183 | 19 | 0 | 1 | 183 | 1 | 183 | 3.47e-122 | 342 |
| MsG0380015195.01.T01 | MTR_3g064780 | 89.444 | 180 | 19 | 0 | 1 | 180 | 1 | 180 | 4.32e-119 | 337 |
| MsG0380015195.01.T01 | MTR_8g432440 | 82.065 | 184 | 32 | 1 | 1 | 183 | 1 | 184 | 8.25e-112 | 316 |
| MsG0380015195.01.T01 | MTR_8g432420 | 82.065 | 184 | 32 | 1 | 1 | 183 | 1 | 184 | 8.25e-112 | 316 |
| MsG0380015195.01.T01 | MTR_8g432470 | 82.609 | 184 | 31 | 1 | 1 | 183 | 1 | 184 | 8.34e-112 | 316 |
| MsG0380015195.01.T01 | MTR_8g432450 | 82.955 | 176 | 28 | 2 | 1 | 174 | 1 | 176 | 1.28e-103 | 295 |
| MsG0380015195.01.T01 | MTR_8g432480 | 51.412 | 177 | 64 | 5 | 5 | 175 | 7 | 167 | 2.20e-48 | 155 |
| MsG0380015195.01.T01 | MTR_7g076000 | 35.714 | 182 | 83 | 9 | 5 | 176 | 2 | 159 | 1.17e-24 | 94.7 |
| MsG0380015195.01.T01 | MTR_8g432400 | 37.705 | 183 | 97 | 7 | 5 | 179 | 2 | 175 | 7.18e-24 | 92.8 |
| MsG0380015195.01.T01 | MTR_8g432370 | 35.165 | 182 | 102 | 5 | 5 | 179 | 2 | 174 | 4.40e-22 | 88.2 |
| MsG0380015195.01.T01 | MTR_8g432390 | 36.066 | 183 | 100 | 6 | 5 | 179 | 2 | 175 | 2.75e-21 | 86.3 |
| MsG0380015195.01.T01 | MTR_8g432350 | 34.973 | 183 | 102 | 6 | 5 | 179 | 2 | 175 | 1.21e-20 | 84.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 54 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAAATGGAGTTATTCATTT+TGG | 0.193044 | 3:+66633601 | MsG0380015195.01.T01:CDS |
| AACTCTAATGGTGCTGTTAT+TGG | 0.212348 | 3:+66633736 | MsG0380015195.01.T01:CDS |
| AATTACCCTAGCGTTATTTA+AGG | 0.221683 | 3:+66633775 | MsG0380015195.01.T01:CDS |
| AACAGCACCATTAGAGTTCT+TGG | 0.255915 | 3:-66633731 | None:intergenic |
| TTCAAAAGTGTTCGCCCTAT+TGG | 0.292098 | 3:+66633481 | MsG0380015195.01.T01:CDS |
| TCAAAAGTGTTCGCCCTATT+GGG | 0.316840 | 3:+66633482 | MsG0380015195.01.T01:CDS |
| GCAATTCTAGCCACCCCAAT+AGG | 0.325926 | 3:-66633496 | None:intergenic |
| TATTCTAAACGGGGCTGAAT+TGG | 0.327706 | 3:+66633528 | MsG0380015195.01.T01:CDS |
| AGTTATGGTGATCGGTTAAC+TGG | 0.339421 | 3:+66633694 | MsG0380015195.01.T01:CDS |
| ATCGGTTACCGTGGCGGAAA+AGG | 0.355914 | 3:+66633651 | MsG0380015195.01.T01:CDS |
| GATTGGGAAGGGAAGCAAAA+TGG | 0.374296 | 3:+66633586 | MsG0380015195.01.T01:CDS |
| CTGTTATTGGTATTCCTGTT+TGG | 0.387184 | 3:+66633749 | MsG0380015195.01.T01:CDS |
| GGTGGCTAGAATTGCCGTTT+CGG | 0.405517 | 3:+66633504 | MsG0380015195.01.T01:CDS |
| CCGTTTCGGATATTCTAAAC+GGG | 0.408061 | 3:+66633518 | MsG0380015195.01.T01:CDS |
| TGGTGTGAGGGTGGTTATGC+AGG | 0.410790 | 3:+66633621 | MsG0380015195.01.T01:CDS |
| TGAGTTATAAACTGAGGGAT+TGG | 0.418159 | 3:+66633569 | MsG0380015195.01.T01:CDS |
| GCCGTTTCGGATATTCTAAA+CGG | 0.426537 | 3:+66633517 | MsG0380015195.01.T01:CDS |
| TCAGTTTATAACTCAACATC+CGG | 0.444784 | 3:-66633561 | None:intergenic |
| GAGTTATAAACTGAGGGATT+GGG | 0.449638 | 3:+66633570 | MsG0380015195.01.T01:CDS |
| CTGAATTGGAAAATTGTTCC+CGG | 0.456171 | 3:+66633542 | MsG0380015195.01.T01:CDS |
| TTATTGGTATTCCTGTTTGG+TGG | 0.478044 | 3:+66633752 | MsG0380015195.01.T01:CDS |
| CAATTCTAGCCACCCCAATA+GGG | 0.482776 | 3:-66633495 | None:intergenic |
| CTAGGGTAATTCCACCAAAC+AGG | 0.484703 | 3:-66633763 | None:intergenic |
| CTCACGTACATTAGAAATAA+CGG | 0.497810 | 3:+66633255 | MsG0380015195.01.T01:CDS |
| ACAAATCCTTAAATAACGCT+AGG | 0.498412 | 3:-66633781 | None:intergenic |
| TAATTGTTACACAATGAAAA+CGG | 0.503129 | 3:+66633342 | MsG0380015195.01.T01:CDS |
| TTAGAAATAACGGTTATATC+CGG | 0.522816 | 3:+66633265 | MsG0380015195.01.T01:CDS |
| ATGGCGGATGGAAAGAGTTA+TGG | 0.523652 | 3:+66633679 | MsG0380015195.01.T01:CDS |
| TGCAAGGTCAATCACATTTG+AGG | 0.534627 | 3:+66633444 | MsG0380015195.01.T01:CDS |
| CGGTTAACTGGAATTGATGT+TGG | 0.538648 | 3:+66633706 | MsG0380015195.01.T01:CDS |
| GATGCATACGTTGTCGTTCG+AGG | 0.545214 | 3:+66633310 | MsG0380015195.01.T01:CDS |
| GTTGGTACCAAGAACTCTAA+TGG | 0.547678 | 3:+66633724 | MsG0380015195.01.T01:CDS |
| GTGAAAGATAATGATGATTG+TGG | 0.551392 | 3:+66633364 | MsG0380015195.01.T01:CDS |
| CCCGTTTAGAATATCCGAAA+CGG | 0.552046 | 3:-66633518 | None:intergenic |
| TGAACATGCCATTGCATGCA+AGG | 0.566598 | 3:+66633428 | MsG0380015195.01.T01:CDS |
| CAAAAGTGTTCGCCCTATTG+GGG | 0.566660 | 3:+66633483 | MsG0380015195.01.T01:CDS |
| ATAAACTGAGGGATTGGGAA+GGG | 0.569199 | 3:+66633575 | MsG0380015195.01.T01:CDS |
| GATGTTGAGTTATAAACTGA+GGG | 0.570650 | 3:+66633564 | MsG0380015195.01.T01:CDS |
| CAAATCCTTAAATAACGCTA+GGG | 0.576601 | 3:-66633780 | None:intergenic |
| ATGGAAAGAGTTATGGTGAT+CGG | 0.595704 | 3:+66633686 | MsG0380015195.01.T01:CDS |
| GTGATTGACCTTGCATGCAA+TGG | 0.597541 | 3:-66633436 | None:intergenic |
| GAAAAGGAAACAATGGCGGA+TGG | 0.603995 | 3:+66633667 | MsG0380015195.01.T01:CDS |
| CGTTATTTCTAATGTACGTG+AGG | 0.605270 | 3:-66633254 | None:intergenic |
| CGTTTCGGATATTCTAAACG+GGG | 0.621740 | 3:+66633519 | MsG0380015195.01.T01:CDS |
| GAAAAGATCGGTTACCGTGG+CGG | 0.623798 | 3:+66633645 | MsG0380015195.01.T01:CDS |
| TGAAAATATTCATGTAACTG+AGG | 0.625588 | 3:+66633288 | MsG0380015195.01.T01:CDS |
| TATAAACTGAGGGATTGGGA+AGG | 0.629947 | 3:+66633574 | MsG0380015195.01.T01:CDS |
| GGTTATGCAGGAGAAAAGAT+CGG | 0.640693 | 3:+66633633 | MsG0380015195.01.T01:CDS |
| AAGTGTTCGCCCTATTGGGG+TGG | 0.645501 | 3:+66633486 | MsG0380015195.01.T01:CDS |
| CAGTTTATAACTCAACATCC+GGG | 0.658129 | 3:-66633560 | None:intergenic |
| CGTGGCGGAAAAGGAAACAA+TGG | 0.664549 | 3:+66633660 | MsG0380015195.01.T01:CDS |
| GGATGTTGAGTTATAAACTG+AGG | 0.678004 | 3:+66633563 | MsG0380015195.01.T01:CDS |
| GGAGAAAAGATCGGTTACCG+TGG | 0.690339 | 3:+66633642 | MsG0380015195.01.T01:CDS |
| GGCGGAAAAGGAAACAATGG+CGG | 0.701876 | 3:+66633663 | MsG0380015195.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATTGTTACACAATGAAAA+CGG | + | Chr3:66633342-66633361 | MsG0380015195.01.T01:CDS | 20.0% |
| ! | TGAAAATATTCATGTAACTG+AGG | + | Chr3:66633288-66633307 | MsG0380015195.01.T01:CDS | 25.0% |
| ! | TTAGAAATAACGGTTATATC+CGG | + | Chr3:66633265-66633284 | MsG0380015195.01.T01:CDS | 25.0% |
| !! | AGTTACATGAATATTTTCAC+CGG | - | Chr3:66633287-66633306 | None:intergenic | 25.0% |
| !! | CAAAATGGAGTTATTCATTT+TGG | + | Chr3:66633601-66633620 | MsG0380015195.01.T01:CDS | 25.0% |
| CTCACGTACATTAGAAATAA+CGG | + | Chr3:66633255-66633274 | MsG0380015195.01.T01:CDS | 30.0% | |
| GATGTTGAGTTATAAACTGA+GGG | + | Chr3:66633564-66633583 | MsG0380015195.01.T01:CDS | 30.0% | |
| GTGAAAGATAATGATGATTG+TGG | + | Chr3:66633364-66633383 | MsG0380015195.01.T01:CDS | 30.0% | |
| TCAGTTTATAACTCAACATC+CGG | - | Chr3:66633564-66633583 | None:intergenic | 30.0% | |
| ! | AAAACTCGAGTTTTCTTTCA+TGG | + | Chr3:66633389-66633408 | MsG0380015195.01.T01:CDS | 30.0% |
| !! | AGTTATTCATTTTGGTGTGA+GGG | + | Chr3:66633609-66633628 | MsG0380015195.01.T01:CDS | 30.0% |
| ATGGAAAGAGTTATGGTGAT+CGG | + | Chr3:66633686-66633705 | MsG0380015195.01.T01:CDS | 35.0% | |
| CAGTTTATAACTCAACATCC+GGG | - | Chr3:66633563-66633582 | None:intergenic | 35.0% | |
| CTGAATTGGAAAATTGTTCC+CGG | + | Chr3:66633542-66633561 | MsG0380015195.01.T01:CDS | 35.0% | |
| CTGTTATTGGTATTCCTGTT+TGG | + | Chr3:66633749-66633768 | MsG0380015195.01.T01:CDS | 35.0% | |
| GAGTTATAAACTGAGGGATT+GGG | + | Chr3:66633570-66633589 | MsG0380015195.01.T01:CDS | 35.0% | |
| GGATGTTGAGTTATAAACTG+AGG | + | Chr3:66633563-66633582 | MsG0380015195.01.T01:CDS | 35.0% | |
| TGAGTTATAAACTGAGGGAT+TGG | + | Chr3:66633569-66633588 | MsG0380015195.01.T01:CDS | 35.0% | |
| TTATTGGTATTCCTGTTTGG+TGG | + | Chr3:66633752-66633771 | MsG0380015195.01.T01:CDS | 35.0% | |
| !! | AACTCTAATGGTGCTGTTAT+TGG | + | Chr3:66633736-66633755 | MsG0380015195.01.T01:CDS | 35.0% |
| !! | CGTTATTTCTAATGTACGTG+AGG | - | Chr3:66633257-66633276 | None:intergenic | 35.0% |
| !! | GAGTTATTCATTTTGGTGTG+AGG | + | Chr3:66633608-66633627 | MsG0380015195.01.T01:CDS | 35.0% |
| AACAGCACCATTAGAGTTCT+TGG | - | Chr3:66633734-66633753 | None:intergenic | 40.0% | |
| AGTTATGGTGATCGGTTAAC+TGG | + | Chr3:66633694-66633713 | MsG0380015195.01.T01:CDS | 40.0% | |
| ATAAACTGAGGGATTGGGAA+GGG | + | Chr3:66633575-66633594 | MsG0380015195.01.T01:CDS | 40.0% | |
| CCGTTTCGGATATTCTAAAC+GGG | + | Chr3:66633518-66633537 | MsG0380015195.01.T01:CDS | 40.0% | |
| CGGTTAACTGGAATTGATGT+TGG | + | Chr3:66633706-66633725 | MsG0380015195.01.T01:CDS | 40.0% | |
| CGTTTCGGATATTCTAAACG+GGG | + | Chr3:66633519-66633538 | MsG0380015195.01.T01:CDS | 40.0% | |
| GCCGTTTCGGATATTCTAAA+CGG | + | Chr3:66633517-66633536 | MsG0380015195.01.T01:CDS | 40.0% | |
| GGTTATGCAGGAGAAAAGAT+CGG | + | Chr3:66633633-66633652 | MsG0380015195.01.T01:CDS | 40.0% | |
| GTTGGTACCAAGAACTCTAA+TGG | + | Chr3:66633724-66633743 | MsG0380015195.01.T01:CDS | 40.0% | |
| TATAAACTGAGGGATTGGGA+AGG | + | Chr3:66633574-66633593 | MsG0380015195.01.T01:CDS | 40.0% | |
| TATTCTAAACGGGGCTGAAT+TGG | + | Chr3:66633528-66633547 | MsG0380015195.01.T01:CDS | 40.0% | |
| TGCAAGGTCAATCACATTTG+AGG | + | Chr3:66633444-66633463 | MsG0380015195.01.T01:CDS | 40.0% | |
| ! | CCCGTTTAGAATATCCGAAA+CGG | - | Chr3:66633521-66633540 | None:intergenic | 40.0% |
| !! | TATTCATTTTGGTGTGAGGG+TGG | + | Chr3:66633612-66633631 | MsG0380015195.01.T01:CDS | 40.0% |
| !! | TCAAAAGTGTTCGCCCTATT+GGG | + | Chr3:66633482-66633501 | MsG0380015195.01.T01:CDS | 40.0% |
| !! | TTCAAAAGTGTTCGCCCTAT+TGG | + | Chr3:66633481-66633500 | MsG0380015195.01.T01:CDS | 40.0% |
| CAATTCTAGCCACCCCAATA+GGG | - | Chr3:66633498-66633517 | None:intergenic | 45.0% | |
| CTAGGGTAATTCCACCAAAC+AGG | - | Chr3:66633766-66633785 | None:intergenic | 45.0% | |
| GATTGGGAAGGGAAGCAAAA+TGG | + | Chr3:66633586-66633605 | MsG0380015195.01.T01:CDS | 45.0% | |
| GTGATTGACCTTGCATGCAA+TGG | - | Chr3:66633439-66633458 | None:intergenic | 45.0% | |
| TGAACATGCCATTGCATGCA+AGG | + | Chr3:66633428-66633447 | MsG0380015195.01.T01:CDS | 45.0% | |
| ! | CATTGTTTCCTTTTCCGCCA+CGG | - | Chr3:66633662-66633681 | None:intergenic | 45.0% |
| ! | GAAAAGGAAACAATGGCGGA+TGG | + | Chr3:66633667-66633686 | MsG0380015195.01.T01:CDS | 45.0% |
| !! | ATGGCGGATGGAAAGAGTTA+TGG | + | Chr3:66633679-66633698 | MsG0380015195.01.T01:CDS | 45.0% |
| !! | CAAAAGTGTTCGCCCTATTG+GGG | + | Chr3:66633483-66633502 | MsG0380015195.01.T01:CDS | 45.0% |
| GAAAAGATCGGTTACCGTGG+CGG | + | Chr3:66633645-66633664 | MsG0380015195.01.T01:CDS | 50.0% | |
| GATGCATACGTTGTCGTTCG+AGG | + | Chr3:66633310-66633329 | MsG0380015195.01.T01:CDS | 50.0% | |
| GCAATTCTAGCCACCCCAAT+AGG | - | Chr3:66633499-66633518 | None:intergenic | 50.0% | |
| GGAGAAAAGATCGGTTACCG+TGG | + | Chr3:66633642-66633661 | MsG0380015195.01.T01:CDS | 50.0% | |
| GGCGGAAAAGGAAACAATGG+CGG | + | Chr3:66633663-66633682 | MsG0380015195.01.T01:CDS | 50.0% | |
| ! | CGTGGCGGAAAAGGAAACAA+TGG | + | Chr3:66633660-66633679 | MsG0380015195.01.T01:CDS | 50.0% |
| ! | GGTGGCTAGAATTGCCGTTT+CGG | + | Chr3:66633504-66633523 | MsG0380015195.01.T01:CDS | 50.0% |
| AAGTGTTCGCCCTATTGGGG+TGG | + | Chr3:66633486-66633505 | MsG0380015195.01.T01:CDS | 55.0% | |
| TGGTGTGAGGGTGGTTATGC+AGG | + | Chr3:66633621-66633640 | MsG0380015195.01.T01:CDS | 55.0% | |
| ! | ATCGGTTACCGTGGCGGAAA+AGG | + | Chr3:66633651-66633670 | MsG0380015195.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 66633244 | 66633795 | 66633244 | ID=MsG0380015195.01;Name=MsG0380015195.01 |
| Chr3 | mRNA | 66633244 | 66633795 | 66633244 | ID=MsG0380015195.01.T01;Parent=MsG0380015195.01;Name=MsG0380015195.01.T01;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|1|1|0|183 |
| Chr3 | exon | 66633244 | 66633795 | 66633244 | ID=MsG0380015195.01.T01:exon:23055;Parent=MsG0380015195.01.T01 |
| Chr3 | CDS | 66633244 | 66633795 | 66633244 | ID=MsG0380015195.01.T01:cds;Parent=MsG0380015195.01.T01 |
| Gene Sequence |
| Protein sequence |
