AlfalfaGEDB | MsG0880043288.01

M. sativa cultivar ZhongmuNo.1 / MsG0880043288.01

KEGG: -
GO: -
PFAM: PF00168

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880043288.01.T01	XP_013444920.1	92.896	183	13	0	1	183	1	183	6.56E-122	352

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880043288.01.T01	A0A072TZI9	92.896	183	13	0	1	183	1	183	3.13e-122	352

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047825.01	MsG0880043288.01	0.804788	1.826160e-49	9.501435e-47
MsG0080048035.01	MsG0880043288.01	0.809216	2.111218e-50	1.232286e-47
MsG0080048177.01	MsG0880043288.01	0.859887	3.030235e-63	8.127918e-60
MsG0080048178.01	MsG0880043288.01	0.856205	3.765155e-62	8.916927e-59
MsG0080048205.01	MsG0880043288.01	0.810925	9.043832e-51	5.523260e-48
MsG0080048210.01	MsG0880043288.01	0.803526	3.342534e-49	1.683609e-46
MsG0080048830.01	MsG0880043288.01	0.801453	8.941327e-49	4.271636e-46
MsG0180000089.01	MsG0880043288.01	0.810121	1.349209e-50	8.065620e-48
MsG0180000157.01	MsG0880043288.01	0.844078	9.446562e-59	1.507987e-55
MsG0180000197.01	MsG0880043288.01	0.818542	1.856909e-52	1.393979e-49
MsG0180000222.01	MsG0880043288.01	0.825619	4.245648e-54	3.889956e-51
MsG0180000267.01	MsG0880043288.01	0.807793	4.248602e-50	2.388412e-47
MsG0180000585.01	MsG0880043288.01	0.831072	2.053257e-55	2.204089e-52
MsG0180000673.01	MsG0880043288.01	0.815706	8.058197e-52	5.596831e-49
MsG0180000821.01	MsG0880043288.01	0.809137	2.195078e-50	1.278542e-47
MsG0180000822.01	MsG0880043288.01	0.804776	1.835991e-49	9.549751e-47
MsG0180001043.01	MsG0880043288.01	0.828544	8.476001e-55	8.450996e-52
MsG0180001154.01	MsG0880043288.01	0.839188	1.839944e-57	2.521432e-54
MsG0180001355.01	MsG0880043288.01	0.802803	4.718004e-49	2.332759e-46
MsG0180001712.01	MsG0880043288.01	0.805770	1.137127e-49	6.067793e-47
MsG0180001921.01	MsG0880043288.01	0.818894	1.544687e-52	1.170983e-49
MsG0180001922.01	MsG0880043288.01	0.863385	2.584944e-64	7.821453e-61
MsG0180002912.01	MsG0880043288.01	0.826341	2.861485e-54	2.676211e-51
MsG0180003088.01	MsG0880043288.01	0.802372	5.789685e-49	2.831432e-46
MsG0180003241.01	MsG0880043288.01	0.858476	8.026534e-63	2.051206e-59
MsG0180003252.01	MsG0880043288.01	0.816403	5.630101e-52	3.985552e-49
MsG0180004116.01	MsG0880043288.01	0.805380	1.373093e-49	7.253435e-47
MsG0180004162.01	MsG0880043288.01	0.826703	2.344775e-54	2.215644e-51
MsG0180004388.01	MsG0880043288.01	0.809646	1.707566e-50	1.008161e-47
MsG0180004762.01	MsG0880043288.01	0.807825	4.182480e-50	2.353156e-47
MsG0180004986.01	MsG0880043288.01	0.818606	1.795618e-52	1.350257e-49
MsG0180005125.01	MsG0880043288.01	0.841481	4.629068e-58	6.811500e-55
MsG0180005773.01	MsG0880043288.01	0.805117	1.558069e-49	8.175483e-47
MsG0180005944.01	MsG0880043288.01	0.819701	1.011290e-52	7.839696e-50
MsG0180005949.01	MsG0880043288.01	0.822580	2.195147e-53	1.844973e-50
MsG0180006016.01	MsG0880043288.01	0.847542	1.082626e-59	1.930880e-56
MsG0180006047.01	MsG0880043288.01	0.802218	6.225608e-49	3.032540e-46
MsG0280006432.01	MsG0880043288.01	0.808993	2.357145e-50	1.367557e-47
MsG0780040505.01	MsG0880043288.01	0.824043	9.996447e-54	8.754855e-51
MsG0780040516.01	MsG0880043288.01	0.841002	6.186791e-58	8.967320e-55
MsG0780040679.01	MsG0880043288.01	0.879931	8.325193e-70	4.600817e-66
MsG0780040726.01	MsG0880043288.01	0.816112	6.539711e-52	4.592932e-49
MsG0780040758.01	MsG0880043288.01	0.824357	8.434451e-54	7.453144e-51
MsG0780040929.01	MsG0880043288.01	0.826221	3.056330e-54	2.848584e-51
MsG0780041357.01	MsG0880043288.01	0.800272	1.557501e-48	7.221750e-46
MsG0780041370.01	MsG0880043288.01	0.809468	1.864424e-50	1.095517e-47
MsG0780041485.01	MsG0880043288.01	0.811037	8.552916e-51	5.238819e-48
MsG0880042053.01	MsG0880043288.01	0.800454	1.430310e-48	6.662552e-46
MsG0880042811.01	MsG0880043288.01	0.800123	1.670426e-48	7.716058e-46
MsG0880042843.01	MsG0880043288.01	0.853393	2.462520e-61	5.312697e-58
MsG0880043001.01	MsG0880043288.01	0.848326	6.581628e-60	1.203471e-56
MsG0880043082.01	MsG0880043288.01	0.816573	5.157181e-52	3.667852e-49
MsG0880043279.01	MsG0880043288.01	0.809513	1.823411e-50	1.072703e-47
MsG0880043285.01	MsG0880043288.01	0.805078	1.588333e-49	8.325628e-47
MsG0880043286.01	MsG0880043288.01	0.848473	5.995311e-60	1.101630e-56
MsG0880043287.01	MsG0880043288.01	0.842100	3.177688e-58	4.767282e-55
MsG0880043288.01	MsG0880043489.01	0.819567	1.085551e-52	8.383821e-50
MsG0880043288.01	MsG0880044039.01	0.812745	3.632074e-51	2.328099e-48
MsG0880043288.01	MsG0880044546.01	0.829106	6.195464e-55	6.279244e-52
MsG0880043288.01	MsG0880045106.01	0.851068	1.129030e-60	2.257674e-57
MsG0880043288.01	MsG0880045290.01	0.817088	3.953672e-52	2.851733e-49
MsG0880043288.01	MsG0880045756.01	0.813274	2.780872e-51	1.807904e-48
MsG0880043288.01	MsG0880045914.01	0.833692	4.607323e-56	5.346038e-53
MsG0880043288.01	MsG0880046058.01	0.822849	1.900423e-53	1.609419e-50
MsG0880043288.01	MsG0880046209.01	0.830309	3.157487e-55	3.314168e-52
MsG0880043288.01	MsG0880046244.01	0.868103	8.369291e-66	2.991322e-62
MsG0880043288.01	MsG0880046329.01	0.853527	2.252549e-61	4.881777e-58
MsG0880043288.01	MsG0880046642.01	0.828354	9.420296e-55	9.340300e-52
MsG0880043288.01	MsG0880046719.01	0.812161	4.873545e-51	3.075687e-48
MsG0880043288.01	MsG0880046731.01	0.846001	2.856319e-59	4.847784e-56
MsG0880043288.01	MsG0880046733.01	0.845438	4.062066e-59	6.769206e-56
MsG0880043288.01	MsG0880046888.01	0.825266	5.148293e-54	4.669771e-51
MsG0880043288.01	MsG0880047019.01	0.819315	1.238647e-52	9.498327e-50
MsG0880043288.01	MsG0880047143.01	0.839289	1.731975e-57	2.380806e-54
MsG0880043288.01	MsG0880047425.01	0.826807	2.215388e-54	2.099578e-51
MsG0880043288.01	MsG0880047444.01	0.838510	2.755602e-57	3.698908e-54
MsG0880043288.01	MsG0880047497.01	0.844745	6.250325e-59	1.018901e-55
MsG0880043288.01	MsG0880047590.01	0.824130	9.533691e-54	8.370797e-51
MsG0280010614.01	MsG0880043288.01	0.805013	1.638666e-49	8.575114e-47
MsG0280010784.01	MsG0880043288.01	0.827203	1.780624e-54	1.707407e-51
MsG0280011105.01	MsG0880043288.01	0.800408	1.461374e-48	6.799577e-46
MsG0280011131.01	MsG0880043288.01	0.813485	2.499911e-51	1.634699e-48
MsG0280011191.01	MsG0880043288.01	0.801126	1.043231e-48	4.943173e-46
MsG0280011235.01	MsG0880043288.01	0.839210	1.815431e-57	2.489647e-54
MsG0280011473.01	MsG0880043288.01	0.811936	5.454472e-51	3.421075e-48
MsG0380011663.01	MsG0880043288.01	0.802289	6.020525e-49	2.938084e-46
MsG0380011754.01	MsG0880043288.01	0.877545	5.766567e-69	2.910458e-65
MsG0380012033.01	MsG0880043288.01	0.813957	1.967268e-51	1.302893e-48
MsG0380012458.01	MsG0880043288.01	0.817313	3.518643e-52	2.553606e-49
MsG0380013047.01	MsG0880043288.01	0.823199	1.574973e-53	1.346983e-50
MsG0380013436.01	MsG0880043288.01	0.820285	7.435906e-53	5.858701e-50
MsG0380014680.01	MsG0880043288.01	0.815626	8.396186e-52	5.819067e-49
MsG0380014913.01	MsG0880043288.01	0.854896	9.066565e-62	2.055649e-58
MsG0680030841.01	MsG0880043288.01	0.825131	5.539594e-54	5.005627e-51
MsG0680031459.01	MsG0880043288.01	0.872056	4.263317e-67	1.758604e-63
MsG0680031538.01	MsG0880043288.01	0.847605	1.040406e-59	1.859145e-56
MsG0680031719.01	MsG0880043288.01	0.814471	1.514616e-51	1.017268e-48
MsG0680032641.01	MsG0880043288.01	0.808650	2.790710e-50	1.604442e-47
MsG0680034450.01	MsG0880043288.01	0.836191	1.081241e-56	1.352743e-53
MsG0680034672.01	MsG0880043288.01	0.871319	7.484434e-67	3.006473e-63
MsG0680035455.01	MsG0880043288.01	0.803708	3.064853e-49	1.550811e-46
MsG0680035460.01	MsG0880043288.01	0.830570	2.725853e-55	2.882917e-52
MsG0680035582.01	MsG0880043288.01	0.813317	2.721072e-51	1.771134e-48
MsG0480018460.01	MsG0880043288.01	0.809077	2.261786e-50	1.315219e-47
MsG0480018635.01	MsG0880043288.01	0.860291	2.288914e-63	6.224516e-60
MsG0480018640.01	MsG0880043288.01	0.808748	2.659126e-50	1.532748e-47
MsG0480018787.01	MsG0880043288.01	0.804488	2.109120e-49	1.088833e-46
MsG0480019289.01	MsG0880043288.01	0.827184	1.800028e-54	1.724974e-51
MsG0480019871.01	MsG0880043288.01	0.800797	1.217591e-48	5.721280e-46
MsG0480020225.01	MsG0880043288.01	0.816989	4.161787e-52	2.993991e-49
MsG0480020242.01	MsG0880043288.01	0.819839	9.406311e-53	7.320123e-50
MsG0480020445.01	MsG0880043288.01	0.850071	2.152011e-60	4.165327e-57
MsG0480020487.01	MsG0880043288.01	0.828102	1.083400e-54	1.066400e-51
MsG0480021240.01	MsG0880043288.01	0.821768	3.387322e-53	2.781566e-50
MsG0480021695.01	MsG0880043288.01	0.814381	1.585927e-51	1.062550e-48
MsG0480022451.01	MsG0880043288.01	0.804192	2.430738e-49	1.245349e-46
MsG0480022479.01	MsG0880043288.01	0.828134	1.064318e-54	1.048586e-51
MsG0480022480.01	MsG0880043288.01	0.836051	1.173659e-56	1.461961e-53
MsG0480022597.01	MsG0880043288.01	0.806934	6.462845e-50	3.552846e-47
MsG0480023088.01	MsG0880043288.01	0.800274	1.556711e-48	7.218238e-46
MsG0480023224.01	MsG0880043288.01	0.829502	4.967379e-55	5.093217e-52
MsG0480023245.01	MsG0880043288.01	0.804293	2.315903e-49	1.189640e-46
MsG0480023270.01	MsG0880043288.01	0.829477	5.037183e-55	5.161154e-52
MsG0480023280.01	MsG0880043288.01	0.858288	9.132461e-63	2.319182e-59
MsG0480023417.01	MsG0880043288.01	0.868457	6.437564e-66	2.330059e-62
MsG0480023427.01	MsG0880043288.01	0.800845	1.190240e-48	5.599799e-46
MsG0480023517.01	MsG0880043288.01	0.863852	1.851365e-64	5.694160e-61
MsG0480023554.01	MsG0880043288.01	0.807346	5.286012e-50	2.937210e-47
MsG0480023903.01	MsG0880043288.01	0.809452	1.879028e-50	1.103624e-47
MsG0480023923.01	MsG0880043288.01	0.840993	6.221150e-58	9.014987e-55
MsG0580024073.01	MsG0880043288.01	0.814214	1.727033e-51	1.151746e-48
MsG0580024114.01	MsG0880043288.01	0.831218	1.891267e-55	2.038895e-52
MsG0580024205.01	MsG0880043288.01	0.834791	2.443967e-56	2.930706e-53
MsG0580024581.01	MsG0880043288.01	0.803841	2.875400e-49	1.460059e-46
MsG0580024682.01	MsG0880043288.01	0.804591	2.006870e-49	1.038839e-46
MsG0580024733.01	MsG0880043288.01	0.805403	1.357785e-49	7.176977e-47
MsG0580025278.01	MsG0880043288.01	0.848559	5.674585e-60	1.045449e-56
MsG0580025328.01	MsG0880043288.01	0.837146	6.173128e-57	7.947155e-54
MsG0580025453.01	MsG0880043288.01	0.836947	6.940491e-57	8.881792e-54
MsG0580025769.01	MsG0880043288.01	0.853704	2.004164e-61	4.368734e-58
MsG0580025907.01	MsG0880043288.01	0.807968	3.900251e-50	2.202576e-47
MsG0580026011.01	MsG0880043288.01	0.837442	5.185067e-57	6.736326e-54
MsG0580026068.01	MsG0880043288.01	0.812287	4.573877e-51	2.896489e-48
MsG0380015181.01	MsG0880043288.01	0.804858	1.765121e-49	9.200878e-47
MsG0380015195.01	MsG0880043288.01	0.809002	2.346534e-50	1.361702e-47
MsG0380015686.01	MsG0880043288.01	0.823862	1.102295e-53	9.606072e-51
MsG0380015878.01	MsG0880043288.01	0.834622	2.694973e-56	3.215752e-53
MsG0380015931.01	MsG0880043288.01	0.851415	9.009246e-61	1.822284e-57
MsG0380015986.01	MsG0880043288.01	0.835817	1.345730e-56	1.664331e-53
MsG0380016223.01	MsG0880043288.01	0.813233	2.839233e-51	1.843690e-48
MsG0380016227.01	MsG0880043288.01	0.801546	8.557703e-49	4.097887e-46
MsG0380016624.01	MsG0880043288.01	0.813440	2.557278e-51	1.670138e-48
MsG0380017158.01	MsG0880043288.01	0.842268	2.868863e-58	4.326620e-55
MsG0380017226.01	MsG0880043288.01	0.811353	7.304746e-51	4.511288e-48
MsG0380017285.01	MsG0880043288.01	0.809680	1.678812e-50	9.920698e-48
MsG0380017286.01	MsG0880043288.01	0.858456	8.136718e-63	2.077918e-59
MsG0380017403.01	MsG0880043288.01	0.801965	7.020954e-49	3.397828e-46
MsG0380017631.01	MsG0880043288.01	0.816374	5.714166e-52	4.041968e-49
MsG0380017940.01	MsG0880043288.01	0.803142	4.013727e-49	2.001692e-46
MsG0480018321.01	MsG0880043288.01	0.831365	1.739340e-55	1.883418e-52
MsG0480018332.01	MsG0880043288.01	0.806509	7.948227e-50	4.321878e-47
MsG0580026596.01	MsG0880043288.01	0.813413	2.592676e-51	1.692017e-48
MsG0580027034.01	MsG0880043288.01	0.822682	2.079264e-53	1.752529e-50
MsG0580027129.01	MsG0880043288.01	0.834909	2.281793e-56	2.746363e-53
MsG0580027345.01	MsG0880043288.01	0.842929	1.915427e-58	2.948752e-55
MsG0580028185.01	MsG0880043288.01	0.819037	1.433534e-52	1.091010e-49
MsG0580028416.01	MsG0880043288.01	0.821757	3.407837e-53	2.797385e-50
MsG0580028453.01	MsG0880043288.01	0.819306	1.244615e-52	9.541752e-50
MsG0580028526.01	MsG0880043288.01	0.802989	4.317061e-49	2.144509e-46
MsG0580028969.01	MsG0880043288.01	0.808100	3.655117e-50	2.071417e-47
MsG0580028980.01	MsG0880043288.01	0.816165	6.364874e-52	4.476565e-49
MsG0580029758.01	MsG0880043288.01	0.846963	1.561383e-59	2.733305e-56
MsG0580029906.01	MsG0880043288.01	0.806154	9.441002e-50	5.087403e-47
MsG0580030235.01	MsG0880043288.01	0.809113	2.221275e-50	1.292954e-47
MsG0680030629.01	MsG0880043288.01	0.829972	3.817299e-55	3.968008e-52
MsG0680030685.01	MsG0880043288.01	0.816169	6.351871e-52	4.467873e-49
MsG0280006595.01	MsG0880043288.01	0.807942	3.950588e-50	2.229461e-47
MsG0280006728.01	MsG0880043288.01	0.803770	2.975131e-49	1.507874e-46
MsG0280006935.01	MsG0880043288.01	0.834237	3.366911e-56	3.971459e-53
MsG0280006936.01	MsG0880043288.01	0.812546	4.014868e-51	2.560129e-48
MsG0280007120.01	MsG0880043288.01	0.806070	9.832142e-50	5.286839e-47
MsG0280007258.01	MsG0880043288.01	0.833218	6.049433e-56	6.921236e-53
MsG0280007272.01	MsG0880043288.01	0.866292	3.172007e-65	1.063626e-61
MsG0280007481.01	MsG0880043288.01	0.828442	8.972303e-55	8.919409e-52
MsG0280007549.01	MsG0880043288.01	0.828792	7.381441e-55	7.412467e-52
MsG0280007714.01	MsG0880043288.01	0.826088	3.287281e-54	3.052154e-51
MsG0280007948.01	MsG0880043288.01	0.805001	1.647987e-49	8.621350e-47
MsG0280008206.01	MsG0880043288.01	0.803501	3.382689e-49	1.702702e-46
MsG0280008220.01	MsG0880043288.01	0.847456	1.143440e-59	2.033648e-56
MsG0280008295.01	MsG0880043288.01	0.843961	1.015255e-58	1.614816e-55
MsG0280008907.01	MsG0880043288.01	0.808817	2.569856e-50	1.484006e-47
MsG0280009235.01	MsG0880043288.01	0.825658	4.157915e-54	3.813742e-51
MsG0280010416.01	MsG0880043288.01	0.850166	2.023709e-60	3.929119e-57
MsG0680035737.01	MsG0880043288.01	0.800571	1.354226e-48	6.326834e-46
MsG0680035844.01	MsG0880043288.01	0.808638	2.806926e-50	1.613263e-47
MsG0780035958.01	MsG0880043288.01	0.837833	4.115237e-57	5.411454e-54
MsG0780036291.01	MsG0880043288.01	0.810351	1.203767e-50	7.239334e-48
MsG0780036582.01	MsG0880043288.01	0.802572	5.264847e-49	2.587710e-46
MsG0780036609.01	MsG0880043288.01	0.804069	2.578106e-49	1.316816e-46
MsG0780036630.01	MsG0880043288.01	0.808641	2.802964e-50	1.611112e-47
MsG0780037258.01	MsG0880043288.01	0.805307	1.422132e-49	7.498337e-47
MsG0780037415.01	MsG0880043288.01	0.803468	3.435860e-49	1.727932e-46
MsG0780037503.01	MsG0880043288.01	0.801946	7.082086e-49	3.425850e-46
MsG0780037515.01	MsG0880043288.01	0.817782	2.758806e-52	2.028142e-49
MsG0780038231.01	MsG0880043288.01	0.800497	1.401618e-48	6.536104e-46
MsG0780038444.01	MsG0880043288.01	0.852272	5.146473e-61	1.069832e-57
MsG0780038829.01	MsG0880043288.01	0.842749	2.138730e-58	3.274067e-55
MsG0780039452.01	MsG0880043288.01	0.800308	1.532018e-48	7.110296e-46
MsG0780039875.01	MsG0880043288.01	0.828224	1.012301e-54	9.999640e-52
MsG0780039900.01	MsG0880043288.01	0.800936	1.140699e-48	5.379102e-46
MsG0780039966.01	MsG0880043288.01	0.809359	1.967173e-50	1.152623e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880043288.01.T01	MTR_8g432490	92.896	183	13	0	1	183	1	183	7.94e-126	352
MsG0880043288.01.T01	MTR_8g432460	87.978	183	22	0	1	183	1	183	3.22e-119	335
MsG0880043288.01.T01	MTR_3g064780	86.111	180	25	0	1	180	1	180	5.04e-112	319
MsG0880043288.01.T01	MTR_8g432440	83.696	184	29	1	1	183	1	184	6.34e-112	317
MsG0880043288.01.T01	MTR_8g432420	83.696	184	29	1	1	183	1	184	6.34e-112	317
MsG0880043288.01.T01	MTR_8g432470	82.609	184	31	1	1	183	1	184	3.08e-109	310
MsG0880043288.01.T01	MTR_8g432450	83.051	177	28	2	1	175	1	177	9.51e-103	293
MsG0880043288.01.T01	MTR_8g432480	51.136	176	64	5	5	174	7	166	3.50e-46	150
MsG0880043288.01.T01	MTR_7g076000	35.556	180	84	8	4	174	1	157	8.57e-24	92.4
MsG0880043288.01.T01	MTR_8g432400	35.870	184	101	6	4	179	1	175	5.38e-21	85.5
MsG0880043288.01.T01	MTR_8g432370	33.880	183	105	5	4	179	1	174	8.67e-20	82.4
MsG0880043288.01.T01	MTR_8g432350	33.702	181	104	5	4	177	1	172	3.59e-19	80.9
MsG0880043288.01.T01	MTR_8g432390	34.783	184	103	6	4	179	1	175	4.87e-19	80.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 45 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAAAATGGAGTTATTCATTT+TGG	0.202103	8:+22004646	MsG0880043288.01.T01:CDS
AACTCTAATGGTGGTGTTAT+TGG	0.227722	8:+22004781	MsG0880043288.01.T01:CDS
AACACCACCATTAGAGTTCT+TGG	0.255915	8:-22004776	None:intergenic
CCTGGGTAATTCCACCTAAA+AGG	0.262534	8:-22004808	None:intergenic
AAAACTCGAGTTTCCTTTCA+TGG	0.292723	8:+22004434	MsG0880043288.01.T01:CDS
GATTGGGAAGGGAGACAAAA+TGG	0.327474	8:+22004631	MsG0880043288.01.T01:CDS
AGTTATGGTGATCGGTTAAC+TGG	0.339421	8:+22004739	MsG0880043288.01.T01:CDS
TTCAAAAGTGTTCGTCCTAT+TGG	0.350867	8:+22004526	MsG0880043288.01.T01:CDS
TGAGTTATAAATTGAGGGAT+TGG	0.355220	8:+22004614	MsG0880043288.01.T01:CDS
ATCGGTTACGGTGGCGGAAA+AGG	0.359368	8:+22004696	MsG0880043288.01.T01:CDS
CCGTTTCAGATATTCTAAAC+AGG	0.374141	8:+22004563	MsG0880043288.01.T01:CDS
GCGATTCTTGCCACGCCAAT+AGG	0.376994	8:-22004541	None:intergenic
ATCATTATCTTTCACCGTCT+TGG	0.400762	8:-22004401	None:intergenic
ACAAATCTTTAAACAATGCC+TGG	0.408723	8:-22004826	None:intergenic
TATTCTAAACAGGGCTGAAT+CGG	0.435899	8:+22004573	MsG0880043288.01.T01:CDS
TGAAAATATTCATGTAACTA+AGG	0.454486	8:+22004333	MsG0880043288.01.T01:CDS
GAGTTATAAATTGAGGGATT+GGG	0.478191	8:+22004615	MsG0880043288.01.T01:CDS
AAAATTTCTCATTCCATGAA+AGG	0.495676	8:-22004447	None:intergenic
TGGTGTGAGGGTGGTTGTGC+CGG	0.499673	8:+22004666	MsG0880043288.01.T01:CDS
CTCACGCACATTAGAAATAA+CGG	0.501464	8:+22004300	MsG0880043288.01.T01:CDS
GATGCATACGTTGTTGTTCG+AGG	0.504923	8:+22004355	MsG0880043288.01.T01:CDS
GTGAAAGATAATGATGATTG+TGG	0.518541	8:+22004409	MsG0880043288.01.T01:CDS
TTAGAAATAACGGTTATATC+CGG	0.522816	8:+22004310	MsG0880043288.01.T01:CDS
ATGGCGGATGGAAAGAGTTA+TGG	0.523652	8:+22004724	MsG0880043288.01.T01:CDS
GTTGGTACCAAGAACTCTAA+TGG	0.534197	8:+22004769	MsG0880043288.01.T01:CDS
GAAAACATCGGTTACGGTGG+CGG	0.535405	8:+22004690	MsG0880043288.01.T01:CDS
CGGTTAACTGGAATTGATGT+TGG	0.538648	8:+22004751	MsG0880043288.01.T01:CDS
ATAAATTGAGGGATTGGGAA+GGG	0.539517	8:+22004620	MsG0880043288.01.T01:CDS
CCTGTTTAGAATATCTGAAA+CGG	0.542041	8:-22004563	None:intergenic
CAAATCTTTAAACAATGCCT+GGG	0.558166	8:-22004825	None:intergenic
TGAAAGATAATGATGATTGT+GGG	0.561848	8:+22004410	MsG0880043288.01.T01:CDS
TGAACATGCCATTGCATGCA+AGG	0.566598	8:+22004473	MsG0880043288.01.T01:CDS
TATAAATTGAGGGATTGGGA+AGG	0.575657	8:+22004619	MsG0880043288.01.T01:CDS
CGTTTCAGATATTCTAAACA+GGG	0.579121	8:+22004564	MsG0880043288.01.T01:CDS
GCCGGAGAAAACATCGGTTA+CGG	0.585557	8:+22004684	MsG0880043288.01.T01:CDS
ATGGAAAGAGTTATGGTGAT+CGG	0.595704	8:+22004731	MsG0880043288.01.T01:CDS
GTGATTGACCTTGCATGCAA+TGG	0.600206	8:-22004481	None:intergenic
GAAAAGGAAACAATGGCGGA+TGG	0.603995	8:+22004712	MsG0880043288.01.T01:CDS
AAGTGTTCGTCCTATTGGCG+TGG	0.646267	8:+22004531	MsG0880043288.01.T01:CDS
GGTTGTGCCGGAGAAAACAT+CGG	0.667699	8:+22004678	MsG0880043288.01.T01:CDS
GGTGGCGGAAAAGGAAACAA+TGG	0.668224	8:+22004705	MsG0880043288.01.T01:CDS
GGAGAAAACATCGGTTACGG+TGG	0.687005	8:+22004687	MsG0880043288.01.T01:CDS
GGCGGAAAAGGAAACAATGG+CGG	0.701876	8:+22004708	MsG0880043288.01.T01:CDS
TACTTGTTACACGACCAAGA+CGG	0.705041	8:+22004387	MsG0880043288.01.T01:CDS
GGTACCAAGAACTCTAATGG+TGG	0.759836	8:+22004772	MsG0880043288.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AATTTTGAGTTATAAATTGA+GGG	+	Chr8:22004609-22004628	MsG0880043288.01.T01:CDS	15.0%
!!	TGAAAATATTCATGTAACTA+AGG	+	Chr8:22004333-22004352	MsG0880043288.01.T01:CDS	20.0%
!!!	GAATTTTGAGTTATAAATTG+AGG	+	Chr8:22004608-22004627	MsG0880043288.01.T01:CDS	20.0%
!	AAAATTTCTCATTCCATGAA+AGG	-	Chr8:22004450-22004469	None:intergenic	25.0%
!	TGAAAGATAATGATGATTGT+GGG	+	Chr8:22004410-22004429	MsG0880043288.01.T01:CDS	25.0%
!	TTAGAAATAACGGTTATATC+CGG	+	Chr8:22004310-22004329	MsG0880043288.01.T01:CDS	25.0%
!!	AGTTACATGAATATTTTCAC+CGG	-	Chr8:22004332-22004351	None:intergenic	25.0%
!!	CAAAATGGAGTTATTCATTT+TGG	+	Chr8:22004646-22004665	MsG0880043288.01.T01:CDS	25.0%
	CGTTTCAGATATTCTAAACA+GGG	+	Chr8:22004564-22004583	MsG0880043288.01.T01:CDS	30.0%
	GAGTTATAAATTGAGGGATT+GGG	+	Chr8:22004615-22004634	MsG0880043288.01.T01:CDS	30.0%
	GTGAAAGATAATGATGATTG+TGG	+	Chr8:22004409-22004428	MsG0880043288.01.T01:CDS	30.0%
	TGAGTTATAAATTGAGGGAT+TGG	+	Chr8:22004614-22004633	MsG0880043288.01.T01:CDS	30.0%
!	CCTGTTTAGAATATCTGAAA+CGG	-	Chr8:22004566-22004585	None:intergenic	30.0%
!	TTATTGGTATTCCTTTTAGG+TGG	+	Chr8:22004797-22004816	MsG0880043288.01.T01:CDS	30.0%
!!	AGTTATTCATTTTGGTGTGA+GGG	+	Chr8:22004654-22004673	MsG0880043288.01.T01:CDS	30.0%
!!	GTGTTATTGGTATTCCTTTT+AGG	+	Chr8:22004794-22004813	MsG0880043288.01.T01:CDS	30.0%
	AAAACTCGAGTTTCCTTTCA+TGG	+	Chr8:22004434-22004453	MsG0880043288.01.T01:CDS	35.0%
	ATAAATTGAGGGATTGGGAA+GGG	+	Chr8:22004620-22004639	MsG0880043288.01.T01:CDS	35.0%
	ATCATTATCTTTCACCGTCT+TGG	-	Chr8:22004404-22004423	None:intergenic	35.0%
	ATGGAAAGAGTTATGGTGAT+CGG	+	Chr8:22004731-22004750	MsG0880043288.01.T01:CDS	35.0%
	CCGTTTCAGATATTCTAAAC+AGG	+	Chr8:22004563-22004582	MsG0880043288.01.T01:CDS	35.0%
	CTCACGCACATTAGAAATAA+CGG	+	Chr8:22004300-22004319	MsG0880043288.01.T01:CDS	35.0%
	TATAAATTGAGGGATTGGGA+AGG	+	Chr8:22004619-22004638	MsG0880043288.01.T01:CDS	35.0%
	TATTCTAAACAGGGCTGAAT+CGG	+	Chr8:22004573-22004592	MsG0880043288.01.T01:CDS	35.0%
!!	AACTCTAATGGTGGTGTTAT+TGG	+	Chr8:22004781-22004800	MsG0880043288.01.T01:CDS	35.0%
!!	GAGTTATTCATTTTGGTGTG+AGG	+	Chr8:22004653-22004672	MsG0880043288.01.T01:CDS	35.0%
!!	TTCAAAAGTGTTCGTCCTAT+TGG	+	Chr8:22004526-22004545	MsG0880043288.01.T01:CDS	35.0%
	AACACCACCATTAGAGTTCT+TGG	-	Chr8:22004779-22004798	None:intergenic	40.0%
	AGTTATGGTGATCGGTTAAC+TGG	+	Chr8:22004739-22004758	MsG0880043288.01.T01:CDS	40.0%
	CGGTTAACTGGAATTGATGT+TGG	+	Chr8:22004751-22004770	MsG0880043288.01.T01:CDS	40.0%
	GTTGGTACCAAGAACTCTAA+TGG	+	Chr8:22004769-22004788	MsG0880043288.01.T01:CDS	40.0%
!	TACTTGTTACACGACCAAGA+CGG	+	Chr8:22004387-22004406	MsG0880043288.01.T01:CDS	40.0%
!!	TATTCATTTTGGTGTGAGGG+TGG	+	Chr8:22004657-22004676	MsG0880043288.01.T01:CDS	40.0%
!!	TGCAAGGTCAATCACTTTTG+AGG	+	Chr8:22004489-22004508	MsG0880043288.01.T01:CDS	40.0%
	CCTGGGTAATTCCACCTAAA+AGG	-	Chr8:22004811-22004830	None:intergenic	45.0%
	GATTGGGAAGGGAGACAAAA+TGG	+	Chr8:22004631-22004650	MsG0880043288.01.T01:CDS	45.0%
	GGTACCAAGAACTCTAATGG+TGG	+	Chr8:22004772-22004791	MsG0880043288.01.T01:CDS	45.0%
	GTGATTGACCTTGCATGCAA+TGG	-	Chr8:22004484-22004503	None:intergenic	45.0%
	TGAACATGCCATTGCATGCA+AGG	+	Chr8:22004473-22004492	MsG0880043288.01.T01:CDS	45.0%
!	ACCGTAACCGATGTTTTCTC+CGG	-	Chr8:22004688-22004707	None:intergenic	45.0%
!	GAAAAGGAAACAATGGCGGA+TGG	+	Chr8:22004712-22004731	MsG0880043288.01.T01:CDS	45.0%
!	GATGCATACGTTGTTGTTCG+AGG	+	Chr8:22004355-22004374	MsG0880043288.01.T01:CDS	45.0%
!!	ATGGCGGATGGAAAGAGTTA+TGG	+	Chr8:22004724-22004743	MsG0880043288.01.T01:CDS	45.0%
!!	CCTTTTAGGTGGAATTACCC+AGG	+	Chr8:22004808-22004827	MsG0880043288.01.T01:CDS	45.0%
	AAGTGTTCGTCCTATTGGCG+TGG	+	Chr8:22004531-22004550	MsG0880043288.01.T01:CDS	50.0%
	GGCGGAAAAGGAAACAATGG+CGG	+	Chr8:22004708-22004727	MsG0880043288.01.T01:CDS	50.0%
	GGTTGTGCCGGAGAAAACAT+CGG	+	Chr8:22004678-22004697	MsG0880043288.01.T01:CDS	50.0%
!	GAAAACATCGGTTACGGTGG+CGG	+	Chr8:22004690-22004709	MsG0880043288.01.T01:CDS	50.0%
!	GCCGGAGAAAACATCGGTTA+CGG	+	Chr8:22004684-22004703	MsG0880043288.01.T01:CDS	50.0%
!	GGAGAAAACATCGGTTACGG+TGG	+	Chr8:22004687-22004706	MsG0880043288.01.T01:CDS	50.0%
!!	GGTGGCGGAAAAGGAAACAA+TGG	+	Chr8:22004705-22004724	MsG0880043288.01.T01:CDS	50.0%
	GCGATTCTTGCCACGCCAAT+AGG	-	Chr8:22004544-22004563	None:intergenic	55.0%
!!	ATCGGTTACGGTGGCGGAAA+AGG	+	Chr8:22004696-22004715	MsG0880043288.01.T01:CDS	55.0%
	TGGTGTGAGGGTGGTTGTGC+CGG	+	Chr8:22004666-22004685	MsG0880043288.01.T01:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	22004289	22004840	22004289	ID=MsG0880043288.01;Name=MsG0880043288.01
Chr8	mRNA	22004289	22004840	22004289	ID=MsG0880043288.01.T01;Parent=MsG0880043288.01;Name=MsG0880043288.01.T01;_AED=0.49;_eAED=0.49;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|183
Chr8	exon	22004289	22004840	22004289	ID=MsG0880043288.01.T01:exon:5821;Parent=MsG0880043288.01.T01
Chr8	CDS	22004289	22004840	22004289	ID=MsG0880043288.01.T01:cds;Parent=MsG0880043288.01.T01

Gene Sequence

>MsG0880043288.01.T01

ATGGGTATGGTCTCACGCACATTAGAAATAACGGTTATATCCGGTGAAAATATTCATGTAACTAAGGATGCATACGTTGTTGTTCGAGGCGAGTCTCTTACTTGTTACACGACCAAGACGGTGAAAGATAATGATGATTGTGGGAAAAACTCGAGTTTCCTTTCATGGAATGAGAAATTTTTGTTGAACATGCCATTGCATGCAAGGTCAATCACTTTTGAGGTGCAATGCAAGAAGTTCAAAAGTGTTCGTCCTATTGGCGTGGCAAGAATCGCCGTTTCAGATATTCTAAACAGGGCTGAATCGGAAAATTGTTCGCGAATTTTGAGTTATAAATTGAGGGATTGGGAAGGGAGACAAAATGGAGTTATTCATTTTGGTGTGAGGGTGGTTGTGCCGGAGAAAACATCGGTTACGGTGGCGGAAAAGGAAACAATGGCGGATGGAAAGAGTTATGGTGATCGGTTAACTGGAATTGATGTTGGTACCAAGAACTCTAATGGTGGTGTTATTGGTATTCCTTTTAGGTGGAATTACCCAGGCATTGTTTAA

Protein sequence

>MsG0880043288.01.T01

MGMVSRTLEITVISGENIHVTKDAYVVVRGESLTCYTTKTVKDNDDCGKNSSFLSWNEKFLLNMPLHARSITFEVQCKKFKSVRPIGVARIAVSDILNRAESENCSRILSYKLRDWEGRQNGVIHFGVRVVVPEKTSVTVAEKETMADGKSYGDRLTGIDVGTKNSNGGVIGIPFRWNYPGIV*