Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015223.01.T01 | XP_003600710.1 | 94.167 | 120 | 7 | 0 | 1 | 120 | 88 | 207 | 3.82E-65 | 209 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015223.01.T01 | Q0WPN8 | 57.6 | 125 | 50 | 1 | 1 | 122 | 99 | 223 | 1.56E-44 | 149 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015223.01.T01 | G7J9C6 | 94.167 | 120 | 7 | 0 | 1 | 120 | 88 | 207 | 1.82e-65 | 209 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380015223.01 | MsG0680031356.01 | 0.800619 | 1.323744e-48 | 6.192100e-46 |
| MsG0380015223.01 | MsG0780041820.01 | 0.802626 | 5.130273e-49 | 2.525145e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015223.01.T01 | MTR_3g065370 | 94.167 | 120 | 7 | 0 | 1 | 120 | 88 | 207 | 4.63e-69 | 209 |
| MsG0380015223.01.T01 | MTR_5g078030 | 65.833 | 120 | 38 | 1 | 1 | 117 | 90 | 209 | 4.84e-44 | 145 |
| MsG0380015223.01.T01 | MTR_8g104990 | 47.107 | 121 | 57 | 2 | 4 | 117 | 90 | 210 | 3.36e-31 | 112 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015223.01.T01 | AT5G37660 | 57.600 | 125 | 50 | 1 | 1 | 122 | 99 | 223 | 1.57e-45 | 149 |
| MsG0380015223.01.T01 | AT5G37660 | 57.600 | 125 | 50 | 1 | 1 | 122 | 99 | 223 | 1.59e-45 | 149 |
| MsG0380015223.01.T01 | AT2G01660 | 57.983 | 119 | 46 | 2 | 3 | 117 | 94 | 212 | 1.34e-41 | 138 |
| MsG0380015223.01.T01 | AT2G01660 | 57.983 | 119 | 46 | 2 | 3 | 117 | 94 | 212 | 1.77e-41 | 139 |
| MsG0380015223.01.T01 | AT2G01660 | 57.983 | 119 | 46 | 2 | 3 | 117 | 94 | 212 | 2.15e-41 | 139 |
| MsG0380015223.01.T01 | AT1G70690 | 40.678 | 118 | 66 | 1 | 4 | 117 | 98 | 215 | 2.91e-26 | 99.8 |
| MsG0380015223.01.T01 | AT3G60720 | 39.024 | 123 | 71 | 2 | 3 | 121 | 97 | 219 | 1.75e-18 | 78.6 |
| MsG0380015223.01.T01 | AT3G60720 | 39.024 | 123 | 71 | 2 | 3 | 121 | 97 | 219 | 3.10e-18 | 78.2 |
| MsG0380015223.01.T01 | AT3G60720 | 39.024 | 123 | 71 | 2 | 3 | 121 | 97 | 219 | 3.41e-18 | 78.2 |
Find 47 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATTATGGAGATATGTTCTT+AGG | 0.218260 | 3:+67037157 | MsG0380015223.01.T01:CDS |
| CGGGTTCAGGTGGGTTGTTT+AGG | 0.228798 | 3:+67037015 | MsG0380015223.01.T01:CDS |
| TTGAGAAATGATTGTCCTTC+TGG | 0.234841 | 3:+67037134 | MsG0380015223.01.T01:CDS |
| TGATTGTCCTTCTGGGATTA+TGG | 0.237076 | 3:+67037142 | MsG0380015223.01.T01:CDS |
| TTGTTTAGGGTTGGTGGTTC+GGG | 0.272429 | 3:+67037029 | MsG0380015223.01.T01:CDS |
| TTGGGTGGGTTGGCGGGTTC+AGG | 0.279854 | 3:+67037002 | MsG0380015223.01.T01:CDS |
| GGGTTCAGGTGGGTTGTTTA+GGG | 0.285383 | 3:+67037016 | MsG0380015223.01.T01:CDS |
| GGTGGGTTGGCGGGTTCAGG+TGG | 0.311303 | 3:+67037005 | MsG0380015223.01.T01:CDS |
| GTTGTTTAGGGTTGGTGGTT+CGG | 0.315244 | 3:+67037028 | MsG0380015223.01.T01:CDS |
| GGTGGGTTGTTTAGGGTTGG+TGG | 0.391830 | 3:+67037023 | MsG0380015223.01.T01:CDS |
| TGCTGTGTTGGGTGGGTTGG+CGG | 0.395709 | 3:+67036995 | MsG0380015223.01.T01:CDS |
| GCTGTGTTGGGTGGGTTGGC+GGG | 0.402006 | 3:+67036996 | MsG0380015223.01.T01:CDS |
| TGGTGGTTCGGGTCAGTTGA+AGG | 0.409087 | 3:+67037040 | MsG0380015223.01.T01:CDS |
| TGAGAAATGATTGTCCTTCT+GGG | 0.414669 | 3:+67037135 | MsG0380015223.01.T01:CDS |
| TCAGGTGGGTTGTTTAGGGT+TGG | 0.416015 | 3:+67037020 | MsG0380015223.01.T01:CDS |
| CCCGGGTCATACCCTACTTC+AGG | 0.420966 | 3:-67036963 | None:intergenic |
| TGAGACAGCGCGTGCAATGC+AGG | 0.450074 | 3:-67036814 | None:intergenic |
| AAGAAATGTGGGCCTGAAGT+AGG | 0.455715 | 3:+67036951 | MsG0380015223.01.T01:CDS |
| AGATGCTGTGTTGGGTGGGT+TGG | 0.456176 | 3:+67036992 | MsG0380015223.01.T01:CDS |
| AACACAGCATCTATCGTTCC+CGG | 0.461385 | 3:-67036981 | None:intergenic |
| ATTATGGAGATATGTTCTTA+GGG | 0.468350 | 3:+67037158 | MsG0380015223.01.T01:CDS |
| ACGATAGATGCTGTGTTGGG+TGG | 0.499063 | 3:+67036987 | MsG0380015223.01.T01:CDS |
| GCAATGCAGGAGGAGCAGTC+TGG | 0.502669 | 3:-67036801 | None:intergenic |
| GGTGGTTCGGGTCAGTTGAA+GGG | 0.521698 | 3:+67037041 | MsG0380015223.01.T01:CDS |
| GGCGGCGTAGTGCAACTAGA+AGG | 0.532882 | 3:+67036867 | MsG0380015223.01.T01:CDS |
| CGCACCGGTGACATATGTCC+TGG | 0.536550 | 3:+67036837 | MsG0380015223.01.T01:CDS |
| ACTACGCCGCCGCACACGCC+AGG | 0.536702 | 3:-67036855 | None:intergenic |
| AGAAATGTGGGCCTGAAGTA+GGG | 0.540842 | 3:+67036952 | MsG0380015223.01.T01:CDS |
| GACAATCATTTCTCAACCGT+CGG | 0.541798 | 3:-67037127 | None:intergenic |
| GGAACGATAGATGCTGTGTT+GGG | 0.547252 | 3:+67036984 | MsG0380015223.01.T01:CDS |
| GTGGGTTGGCGGGTTCAGGT+GGG | 0.556632 | 3:+67037006 | MsG0380015223.01.T01:CDS |
| CGATAGATGCTGTGTTGGGT+GGG | 0.557400 | 3:+67036988 | MsG0380015223.01.T01:CDS |
| GCAGGAGGAGCAGTCTGGCA+TGG | 0.570950 | 3:-67036796 | None:intergenic |
| GCCTGAAGTAGGGTATGACC+CGG | 0.573127 | 3:+67036962 | MsG0380015223.01.T01:CDS |
| TTCGGGTCAGTTGAAGGGAA+TGG | 0.573592 | 3:+67037046 | MsG0380015223.01.T01:CDS |
| GCACGCGCTGTCTCACGCAC+CGG | 0.588396 | 3:+67036822 | MsG0380015223.01.T01:CDS |
| TGTCAAGATTGTAGTTTCAG+AGG | 0.596202 | 3:+67037101 | MsG0380015223.01.T01:CDS |
| GGGAACGATAGATGCTGTGT+TGG | 0.602336 | 3:+67036983 | MsG0380015223.01.T01:CDS |
| ACACAGCATCTATCGTTCCC+GGG | 0.603589 | 3:-67036980 | None:intergenic |
| AAGGGAATGGCACAGTGTGT+TGG | 0.615028 | 3:+67037059 | MsG0380015223.01.T01:CDS |
| ATATGTCCTGGCGTGTGCGG+CGG | 0.627188 | 3:+67036849 | MsG0380015223.01.T01:CDS |
| GTAGTTTCAGAGGCAACCGA+CGG | 0.641265 | 3:+67037111 | MsG0380015223.01.T01:CDS |
| GACAGCGCGTGCAATGCAGG+AGG | 0.641294 | 3:-67036811 | None:intergenic |
| CATATCTCCATAATCCCAGA+AGG | 0.647508 | 3:-67037149 | None:intergenic |
| CACGCCAGGACATATGTCAC+CGG | 0.653459 | 3:-67036841 | None:intergenic |
| GACATATGTCCTGGCGTGTG+CGG | 0.669580 | 3:+67036846 | MsG0380015223.01.T01:CDS |
| CCTGAAGTAGGGTATGACCC+GGG | 0.716639 | 3:+67036963 | MsG0380015223.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATGATAATGTTAAGTTTTT+AGG | + | Chr3:67036903-67036922 | MsG0380015223.01.T01:CDS | 15.0% |
| !!! | TAATGTTAAGTTTTTAGGCA+TGG | + | Chr3:67036908-67036927 | MsG0380015223.01.T01:CDS | 25.0% |
| !!! | ACGATTGTTTTGAAGAAATG+TGG | + | Chr3:67036939-67036958 | MsG0380015223.01.T01:CDS | 30.0% |
| !!! | CGATTGTTTTGAAGAAATGT+GGG | + | Chr3:67036940-67036959 | MsG0380015223.01.T01:CDS | 30.0% |
| TGAGAAATGATTGTCCTTCT+GGG | + | Chr3:67037135-67037154 | MsG0380015223.01.T01:CDS | 35.0% | |
| TGTCAAGATTGTAGTTTCAG+AGG | + | Chr3:67037101-67037120 | MsG0380015223.01.T01:CDS | 35.0% | |
| TTGAGAAATGATTGTCCTTC+TGG | + | Chr3:67037134-67037153 | MsG0380015223.01.T01:CDS | 35.0% | |
| CATATCTCCATAATCCCAGA+AGG | - | Chr3:67037152-67037171 | None:intergenic | 40.0% | |
| GACAATCATTTCTCAACCGT+CGG | - | Chr3:67037130-67037149 | None:intergenic | 40.0% | |
| !! | TGATTGTCCTTCTGGGATTA+TGG | + | Chr3:67037142-67037161 | MsG0380015223.01.T01:CDS | 40.0% |
| !! | TGTTGGCGATTTGAGTTTTG+TGG | + | Chr3:67037076-67037095 | MsG0380015223.01.T01:CDS | 40.0% |
| AACACAGCATCTATCGTTCC+CGG | - | Chr3:67036984-67037003 | None:intergenic | 45.0% | |
| AAGAAATGTGGGCCTGAAGT+AGG | + | Chr3:67036951-67036970 | MsG0380015223.01.T01:CDS | 45.0% | |
| AGAAATGTGGGCCTGAAGTA+GGG | + | Chr3:67036952-67036971 | MsG0380015223.01.T01:CDS | 45.0% | |
| GGAACGATAGATGCTGTGTT+GGG | + | Chr3:67036984-67037003 | MsG0380015223.01.T01:CDS | 45.0% | |
| !! | GTTGTTTAGGGTTGGTGGTT+CGG | + | Chr3:67037028-67037047 | MsG0380015223.01.T01:CDS | 45.0% |
| !! | TTGTTTAGGGTTGGTGGTTC+GGG | + | Chr3:67037029-67037048 | MsG0380015223.01.T01:CDS | 45.0% |
| AAGGGAATGGCACAGTGTGT+TGG | + | Chr3:67037059-67037078 | MsG0380015223.01.T01:CDS | 50.0% | |
| ACACAGCATCTATCGTTCCC+GGG | - | Chr3:67036983-67037002 | None:intergenic | 50.0% | |
| ACGATAGATGCTGTGTTGGG+TGG | + | Chr3:67036987-67037006 | MsG0380015223.01.T01:CDS | 50.0% | |
| CGATAGATGCTGTGTTGGGT+GGG | + | Chr3:67036988-67037007 | MsG0380015223.01.T01:CDS | 50.0% | |
| GGGAACGATAGATGCTGTGT+TGG | + | Chr3:67036983-67037002 | MsG0380015223.01.T01:CDS | 50.0% | |
| GTAGTTTCAGAGGCAACCGA+CGG | + | Chr3:67037111-67037130 | MsG0380015223.01.T01:CDS | 50.0% | |
| TTCGGGTCAGTTGAAGGGAA+TGG | + | Chr3:67037046-67037065 | MsG0380015223.01.T01:CDS | 50.0% | |
| !! | GGGTTCAGGTGGGTTGTTTA+GGG | + | Chr3:67037016-67037035 | MsG0380015223.01.T01:CDS | 50.0% |
| !! | TCAGGTGGGTTGTTTAGGGT+TGG | + | Chr3:67037020-67037039 | MsG0380015223.01.T01:CDS | 50.0% |
| AGATGCTGTGTTGGGTGGGT+TGG | + | Chr3:67036992-67037011 | MsG0380015223.01.T01:CDS | 55.0% | |
| CACGCCAGGACATATGTCAC+CGG | - | Chr3:67036844-67036863 | None:intergenic | 55.0% | |
| CCTGAAGTAGGGTATGACCC+GGG | + | Chr3:67036963-67036982 | MsG0380015223.01.T01:CDS | 55.0% | |
| GACATATGTCCTGGCGTGTG+CGG | + | Chr3:67036846-67036865 | MsG0380015223.01.T01:CDS | 55.0% | |
| GCCTGAAGTAGGGTATGACC+CGG | + | Chr3:67036962-67036981 | MsG0380015223.01.T01:CDS | 55.0% | |
| ! | GGTGGTTCGGGTCAGTTGAA+GGG | + | Chr3:67037041-67037060 | MsG0380015223.01.T01:CDS | 55.0% |
| ! | TGGTGGTTCGGGTCAGTTGA+AGG | + | Chr3:67037040-67037059 | MsG0380015223.01.T01:CDS | 55.0% |
| !! | CGGGTTCAGGTGGGTTGTTT+AGG | + | Chr3:67037015-67037034 | MsG0380015223.01.T01:CDS | 55.0% |
| !! | GGTGGGTTGTTTAGGGTTGG+TGG | + | Chr3:67037023-67037042 | MsG0380015223.01.T01:CDS | 55.0% |
| ATATGTCCTGGCGTGTGCGG+CGG | + | Chr3:67036849-67036868 | MsG0380015223.01.T01:CDS | 60.0% | |
| CCCGGGTCATACCCTACTTC+AGG | - | Chr3:67036966-67036985 | None:intergenic | 60.0% | |
| GCAATGCAGGAGGAGCAGTC+TGG | - | Chr3:67036804-67036823 | None:intergenic | 60.0% | |
| GGCGGCGTAGTGCAACTAGA+AGG | + | Chr3:67036867-67036886 | MsG0380015223.01.T01:CDS | 60.0% | |
| TGAGACAGCGCGTGCAATGC+AGG | - | Chr3:67036817-67036836 | None:intergenic | 60.0% | |
| ! | CGCACCGGTGACATATGTCC+TGG | + | Chr3:67036837-67036856 | MsG0380015223.01.T01:CDS | 60.0% |
| !! | TGCTGTGTTGGGTGGGTTGG+CGG | + | Chr3:67036995-67037014 | MsG0380015223.01.T01:CDS | 60.0% |
| GACAGCGCGTGCAATGCAGG+AGG | - | Chr3:67036814-67036833 | None:intergenic | 65.0% | |
| !! | GCTGTGTTGGGTGGGTTGGC+GGG | + | Chr3:67036996-67037015 | MsG0380015223.01.T01:CDS | 65.0% |
| !! | GTGGGTTGGCGGGTTCAGGT+GGG | + | Chr3:67037006-67037025 | MsG0380015223.01.T01:CDS | 65.0% |
| !! | TTGGGTGGGTTGGCGGGTTC+AGG | + | Chr3:67037002-67037021 | MsG0380015223.01.T01:CDS | 65.0% |
| GCACGCGCTGTCTCACGCAC+CGG | + | Chr3:67036822-67036841 | MsG0380015223.01.T01:CDS | 70.0% | |
| !! | GGTGGGTTGGCGGGTTCAGG+TGG | + | Chr3:67037005-67037024 | MsG0380015223.01.T01:CDS | 70.0% |
| ACTACGCCGCCGCACACGCC+AGG | - | Chr3:67036858-67036877 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 67036798 | 67037178 | 67036798 | ID=MsG0380015223.01;Name=MsG0380015223.01 |
| Chr3 | mRNA | 67036798 | 67037178 | 67036798 | ID=MsG0380015223.01.T01;Parent=MsG0380015223.01;Name=MsG0380015223.01.T01;_AED=0.27;_eAED=0.27;_QI=0|-1|0|1|-1|1|1|0|126 |
| Chr3 | exon | 67036798 | 67037178 | 67036798 | ID=MsG0380015223.01.T01:exon:0;Parent=MsG0380015223.01.T01 |
| Chr3 | CDS | 67036798 | 67037178 | 67036798 | ID=MsG0380015223.01.T01:cds;Parent=MsG0380015223.01.T01 |
| Gene Sequence |
| Protein sequence |
