Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380014289.01 | MsG0380015232.01 | 0.803113 | 4.069891e-49 | 2.028149e-46 |
| MsG0380015232.01 | MsG0880043592.01 | 0.804019 | 2.640312e-49 | 1.346816e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 25 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCATCCATACTTCTTGTTC+TGG | 0.244658 | 3:+67132095 | None:intergenic |
| CCTCTGTCTCTGGAAGAAAA+AGG | 0.375415 | 3:-67131917 | MsG0380015232.01.T01:CDS |
| TTGAACCAGAACAAGAAGTA+TGG | 0.395877 | 3:-67132100 | MsG0380015232.01.T01:CDS |
| AAGAAGTATGGATGAAATTG+TGG | 0.416795 | 3:-67132088 | MsG0380015232.01.T01:CDS |
| TGCAAGTGCAAGAAGAGAAT+GGG | 0.447300 | 3:-67131944 | MsG0380015232.01.T01:CDS |
| GAGATTATATTAAAGAAAGA+TGG | 0.449227 | 3:-67132032 | MsG0380015232.01.T01:CDS |
| CTTTAATATAATCTCCCATT+CGG | 0.467201 | 3:+67132040 | None:intergenic |
| TCAAGCATAAAAGCAGTCAT+AGG | 0.467225 | 3:+67132191 | None:intergenic |
| AGATTATATTAAAGAAAGAT+GGG | 0.470782 | 3:-67132031 | MsG0380015232.01.T01:CDS |
| GTGCAAGTGCAAGAAGAGAA+TGG | 0.477386 | 3:-67131945 | MsG0380015232.01.T01:CDS |
| GCTTGACAATTTGAAAACAT+TGG | 0.478648 | 3:-67132174 | MsG0380015232.01.T01:CDS |
| AATGGGGATACCTCTGTCTC+TGG | 0.495516 | 3:-67131927 | MsG0380015232.01.T01:CDS |
| AGCTCTGCAAGTGCAAGTGC+AGG | 0.502396 | 3:-67132005 | MsG0380015232.01.T01:CDS |
| GCAAGTGCAAGTGCAGGTGC+AGG | 0.509754 | 3:-67131999 | MsG0380015232.01.T01:CDS |
| GTGCAGGTGCTGGTACAAGC+AGG | 0.522259 | 3:-67131983 | MsG0380015232.01.T01:CDS |
| AGAAGTATGGATGAAATTGT+GGG | 0.525701 | 3:-67132087 | MsG0380015232.01.T01:CDS |
| TAAAACAAGTGGAGACCGAA+TGG | 0.537916 | 3:-67132055 | MsG0380015232.01.T01:CDS |
| GGATAATGATTTAAAACAAG+TGG | 0.548998 | 3:-67132066 | MsG0380015232.01.T01:CDS |
| AAAACAAGTGGAGACCGAAT+GGG | 0.555113 | 3:-67132054 | MsG0380015232.01.T01:CDS |
| GCAAGTGCAGGTGCAGGTGC+TGG | 0.570310 | 3:-67131993 | MsG0380015232.01.T01:CDS |
| GAAAGGAAAAGATGCGCTAG+AGG | 0.614097 | 3:-67131895 | MsG0380015232.01.T01:CDS |
| AAAGGAAAAGATGCGCTAGA+GGG | 0.639755 | 3:-67131894 | MsG0380015232.01.T01:CDS |
| ATTGGAGTCTGAACTAACAC+AGG | 0.645882 | 3:-67132156 | MsG0380015232.01.T01:CDS |
| GATTATATTAAAGAAAGATG+GGG | 0.650075 | 3:-67132030 | MsG0380015232.01.T01:CDS |
| GCAAGTGCAAGAAGAGAATG+GGG | 0.669831 | 3:-67131943 | MsG0380015232.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGATTATATTAAAGAAAGAT+GGG | - | Chr3:67132047-67132066 | MsG0380015232.01.T01:CDS | 15.0% |
| !! | GAGATTATATTAAAGAAAGA+TGG | - | Chr3:67132046-67132065 | MsG0380015232.01.T01:CDS | 20.0% |
| !! | GATTATATTAAAGAAAGATG+GGG | - | Chr3:67132048-67132067 | MsG0380015232.01.T01:CDS | 20.0% |
| ! | CTTTAATATAATCTCCCATT+CGG | + | Chr3:67132041-67132060 | None:intergenic | 25.0% |
| !! | GGATAATGATTTAAAACAAG+TGG | - | Chr3:67132012-67132031 | MsG0380015232.01.T01:CDS | 25.0% |
| GCTTGACAATTTGAAAACAT+TGG | - | Chr3:67131904-67131923 | MsG0380015232.01.T01:CDS | 30.0% | |
| ! | AAGAAGTATGGATGAAATTG+TGG | - | Chr3:67131990-67132009 | MsG0380015232.01.T01:CDS | 30.0% |
| ! | AGAAGTATGGATGAAATTGT+GGG | - | Chr3:67131991-67132010 | MsG0380015232.01.T01:CDS | 30.0% |
| TTCATCCATACTTCTTGTTC+TGG | + | Chr3:67131986-67132005 | None:intergenic | 35.0% | |
| TTGAACCAGAACAAGAAGTA+TGG | - | Chr3:67131978-67131997 | MsG0380015232.01.T01:CDS | 35.0% | |
| AAAACAAGTGGAGACCGAAT+GGG | - | Chr3:67132024-67132043 | MsG0380015232.01.T01:CDS | 40.0% | |
| AAAGGAAAAGATGCGCTAGA+GGG | - | Chr3:67132184-67132203 | MsG0380015232.01.T01:CDS | 40.0% | |
| ATTGGAGTCTGAACTAACAC+AGG | - | Chr3:67131922-67131941 | MsG0380015232.01.T01:CDS | 40.0% | |
| TAAAACAAGTGGAGACCGAA+TGG | - | Chr3:67132023-67132042 | MsG0380015232.01.T01:CDS | 40.0% | |
| TGCAAGTGCAAGAAGAGAAT+GGG | - | Chr3:67132134-67132153 | MsG0380015232.01.T01:CDS | 40.0% | |
| ! | GTCTCTGGAAGAAAAAGGAA+AGG | - | Chr3:67132166-67132185 | MsG0380015232.01.T01:CDS | 40.0% |
| GAAAGGAAAAGATGCGCTAG+AGG | - | Chr3:67132183-67132202 | MsG0380015232.01.T01:CDS | 45.0% | |
| GCAAGTGCAAGAAGAGAATG+GGG | - | Chr3:67132135-67132154 | MsG0380015232.01.T01:CDS | 45.0% | |
| GTGCAAGTGCAAGAAGAGAA+TGG | - | Chr3:67132133-67132152 | MsG0380015232.01.T01:CDS | 45.0% | |
| ! | CCTCTGTCTCTGGAAGAAAA+AGG | - | Chr3:67132161-67132180 | MsG0380015232.01.T01:CDS | 45.0% |
| ! | CCTTTTTCTTCCAGAGACAG+AGG | + | Chr3:67132164-67132183 | None:intergenic | 45.0% |
| AATGGGGATACCTCTGTCTC+TGG | - | Chr3:67132151-67132170 | MsG0380015232.01.T01:CDS | 50.0% | |
| AGCTCTGCAAGTGCAAGTGC+AGG | - | Chr3:67132073-67132092 | MsG0380015232.01.T01:CDS | 55.0% | |
| GCAAGTGCAAGTGCAGGTGC+AGG | - | Chr3:67132079-67132098 | MsG0380015232.01.T01:CDS | 60.0% | |
| !! | GTGCAGGTGCTGGTACAAGC+AGG | - | Chr3:67132095-67132114 | MsG0380015232.01.T01:CDS | 60.0% |
| GCAAGTGCAGGTGCAGGTGC+TGG | - | Chr3:67132085-67132104 | MsG0380015232.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 67131890 | 67132210 | 67131890 | ID=MsG0380015232.01;Name=MsG0380015232.01 |
| Chr3 | mRNA | 67131890 | 67132210 | 67131890 | ID=MsG0380015232.01.T01;Parent=MsG0380015232.01;Name=MsG0380015232.01.T01;_AED=0.39;_eAED=0.39;_QI=0|-1|0|1|-1|1|1|0|106 |
| Chr3 | exon | 67131890 | 67132210 | 67131890 | ID=MsG0380015232.01.T01:exon:23161;Parent=MsG0380015232.01.T01 |
| Chr3 | CDS | 67131890 | 67132210 | 67131890 | ID=MsG0380015232.01.T01:cds;Parent=MsG0380015232.01.T01 |
| Gene Sequence |
| Protein sequence |
