AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880043592.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043592.01.T01 MTR_7g029510 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880043592.01.T01 MTR_7g099610 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880043592.01.T01 MTR_5g063620 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880043592.01.T01 MTR_4g061827 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880043592.01.T01 MTR_4g061920 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880043592.01.T01 MTR_3g462400 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880043592.01.T01 MTR_3g054380 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880043592.01.T01 MTR_3g462730 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880043592.01.T01 MTR_3g462510 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880043592.01.T01 MTR_3g070920 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880043592.01.T01 MTR_3g061440 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880043592.01.T01 MTR_3g462410 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880043592.01.T01 MTR_2g096100 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880043592.01.T01 MTR_8g038460 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880043592.01.T01 MTR_4g128150 100.000 102 0 0 1 102 1 102 1.74e-66 204
MsG0880043592.01.T01 MTR_7g029520 84.000 50 8 0 27 76 1 50 2.23e-21 87.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043592.01.T01 AT5G59970 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0880043592.01.T01 AT5G59690 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0880043592.01.T01 AT5G59970 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0880043592.01.T01 AT3G46320 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0880043592.01.T01 AT3G45930 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0880043592.01.T01 AT3G53730 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0880043592.01.T01 AT2G28740 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0880043592.01.T01 AT1G07660 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0880043592.01.T01 AT1G07820 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0880043592.01.T01 AT1G07820 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0880043592.01.T01 AT1G07660 83.495 103 0 1 1 103 1 86 4.52e-51 155

Find 29 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAAGGACTTTGTATGGTTT+CGG 0.343848 8:-27400012 MsG0880043592.01.T01:CDS
GGAAAGGGAGGTAAGGGATT+AGG 0.384736 8:-27400282 MsG0880043592.01.T01:CDS
TCGCTGGCTTTGTGATTCCT+TGG 0.395127 8:+27400214 None:intergenic
AGACAAGGAAGGACTTTGTA+TGG 0.433181 8:-27400018 MsG0880043592.01.T01:CDS
CTCGCTAATCGACGAATCGC+TGG 0.450047 8:+27400198 None:intergenic
GGTAAGGGATTAGGAAAGGG+AGG 0.470949 8:-27400273 MsG0880043592.01.T01:CDS
GATTCTCCATTGAAAATGTC+AGG 0.487679 8:-27400309 None:intergenic
AGGACGTGGAAAGGGAGGTA+AGG 0.498386 8:-27400289 MsG0880043592.01.T01:CDS
GGAGGTAAGGGATTAGGAAA+GGG 0.512610 8:-27400276 MsG0880043592.01.T01:CDS
GGGAGGTAAGGGATTAGGAA+AGG 0.522188 8:-27400277 MsG0880043592.01.T01:CDS
GAGGAAGACGGTTACTGCCA+TGG 0.539140 8:-27400061 MsG0880043592.01.T01:CDS
CCATTGAAAATGTCAGGACG+TGG 0.548627 8:-27400303 None:intergenic
GAAAATGTCAGGACGTGGAA+AGG 0.550208 8:-27400298 None:intergenic
AAAATGTCAGGACGTGGAAA+GGG 0.553387 8:-27400297 MsG0880043592.01.T01:CDS
GGACGTGGAAAGGGAGGTAA+GGG 0.565597 8:-27400288 MsG0880043592.01.T01:CDS
CGTCGATTAGCGAGAAGAGG+AGG 0.585804 8:-27400189 MsG0880043592.01.T01:CDS
CAAAGCATAAACAACATCCA+TGG 0.598965 8:+27400044 None:intergenic
AGGACTTTGTATGGTTTCGG+AGG 0.599906 8:-27400009 MsG0880043592.01.T01:CDS
ATGCTTTGAAGAGACAAGGA+AGG 0.603293 8:-27400029 MsG0880043592.01.T01:CDS
ATTCGTCGATTAGCGAGAAG+AGG 0.605618 8:-27400192 MsG0880043592.01.T01:CDS
GTTCTTCGCGACAACATCCA+AGG 0.612538 8:-27400231 MsG0880043592.01.T01:CDS
GGAGGTGTGAAGAGAATCAG+CGG 0.613587 8:-27400171 MsG0880043592.01.T01:CDS
GTTTATGCTTTGAAGAGACA+AGG 0.627217 8:-27400033 MsG0880043592.01.T01:CDS
TGAACATGCGAGGAGGAAGA+CGG 0.628798 8:-27400073 MsG0880043592.01.T01:CDS
ATGTCAGGACGTGGAAAGGG+AGG 0.638369 8:-27400294 MsG0880043592.01.T01:CDS
GAGGTGTGAAGAGAATCAGC+GGG 0.664332 8:-27400170 MsG0880043592.01.T01:CDS
TTGATTTATGAAGAAACTCG+CGG 0.688782 8:-27400147 MsG0880043592.01.T01:CDS
CGTATACTGAACATGCGAGG+AGG 0.708048 8:-27400080 MsG0880043592.01.T01:CDS
TTACGTATACTGAACATGCG+AGG 0.714123 8:-27400083 MsG0880043592.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TTGATTTATGAAGAAACTCG+CGG - Chr8:27400152-27400171 MsG0880043592.01.T01:CDS 30.0%
CAAAGCATAAACAACATCCA+TGG + Chr8:27400258-27400277 None:intergenic 35.0%
!! GTTTATGCTTTGAAGAGACA+AGG - Chr8:27400266-27400285 MsG0880043592.01.T01:CDS 35.0%
AGACAAGGAAGGACTTTGTA+TGG - Chr8:27400281-27400300 MsG0880043592.01.T01:CDS 40.0%
GGAAGGACTTTGTATGGTTT+CGG - Chr8:27400287-27400306 MsG0880043592.01.T01:CDS 40.0%
TTACGTATACTGAACATGCG+AGG - Chr8:27400216-27400235 MsG0880043592.01.T01:CDS 40.0%
! CGGTGTGTTGAAGATTTTCT+TGG - Chr8:27400172-27400191 MsG0880043592.01.T01:CDS 40.0%
!! ATGCTTTGAAGAGACAAGGA+AGG - Chr8:27400270-27400289 MsG0880043592.01.T01:CDS 40.0%
AGGACTTTGTATGGTTTCGG+AGG - Chr8:27400290-27400309 MsG0880043592.01.T01:CDS 45.0%
GGAGGTAAGGGATTAGGAAA+GGG - Chr8:27400023-27400042 MsG0880043592.01.T01:CDS 45.0%
! ATTCGTCGATTAGCGAGAAG+AGG - Chr8:27400107-27400126 MsG0880043592.01.T01:CDS 45.0%
CGTATACTGAACATGCGAGG+AGG - Chr8:27400219-27400238 MsG0880043592.01.T01:CDS 50.0%
GAGGTGTGAAGAGAATCAGC+GGG - Chr8:27400129-27400148 MsG0880043592.01.T01:CDS 50.0%
GGAAAGGGAGGTAAGGGATT+AGG - Chr8:27400017-27400036 MsG0880043592.01.T01:CDS 50.0%
GGGAGGTAAGGGATTAGGAA+AGG - Chr8:27400022-27400041 MsG0880043592.01.T01:CDS 50.0%
GGTAAGGGATTAGGAAAGGG+AGG - Chr8:27400026-27400045 MsG0880043592.01.T01:CDS 50.0%
GTTCTTCGCGACAACATCCA+AGG - Chr8:27400068-27400087 MsG0880043592.01.T01:CDS 50.0%
TGAACATGCGAGGAGGAAGA+CGG - Chr8:27400226-27400245 MsG0880043592.01.T01:CDS 50.0%
! GGAGGTGTGAAGAGAATCAG+CGG - Chr8:27400128-27400147 MsG0880043592.01.T01:CDS 50.0%
!! TCGCTGGCTTTGTGATTCCT+TGG + Chr8:27400088-27400107 None:intergenic 50.0%
AGGACGTGGAAAGGGAGGTA+AGG - Chr8:27400010-27400029 MsG0880043592.01.T01:CDS 55.0%
ATGTCAGGACGTGGAAAGGG+AGG - Chr8:27400005-27400024 MsG0880043592.01.T01:CDS 55.0%
CTCGCTAATCGACGAATCGC+TGG + Chr8:27400104-27400123 None:intergenic 55.0%
GAGGAAGACGGTTACTGCCA+TGG - Chr8:27400238-27400257 MsG0880043592.01.T01:CDS 55.0%
GGACGTGGAAAGGGAGGTAA+GGG - Chr8:27400011-27400030 MsG0880043592.01.T01:CDS 55.0%
! CGTCGATTAGCGAGAAGAGG+AGG - Chr8:27400110-27400129 MsG0880043592.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr8 gene 27400005 27400316 27400005 ID=MsG0880043592.01;Name=MsG0880043592.01
Chr8 mRNA 27400005 27400316 27400005 ID=MsG0880043592.01.T01;Parent=MsG0880043592.01;Name=MsG0880043592.01.T01;_AED=0.46;_eAED=0.46;_QI=0|-1|0|1|-1|1|1|0|103
Chr8 exon 27400005 27400316 27400005 ID=MsG0880043592.01.T01:exon:8696;Parent=MsG0880043592.01.T01
Chr8 CDS 27400005 27400316 27400005 ID=MsG0880043592.01.T01:cds;Parent=MsG0880043592.01.T01
Gene Sequence

>MsG0880043592.01.T01

ATGTCAGGACGTGGAAAGGGAGGTAAGGGATTAGGAAAGGGAGGTGCAAAGAGACATCGCAAAGTTCTTCGCGACAACATCCAAGGAATCACAAAGCCAGCGATTCGTCGATTAGCGAGAAGAGGAGGTGTGAAGAGAATCAGCGGGTTGATTTATGAAGAAACTCGCGGTGTGTTGAAGATTTTCTTGGAGAATGTGATAAGAGATGCTGTTACGTATACTGAACATGCGAGGAGGAAGACGGTTACTGCCATGGATGTTGTTTATGCTTTGAAGAGACAAGGAAGGACTTTGTATGGTTTCGGAGGTTGA

Protein sequence

>MsG0880043592.01.T01

MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG*