Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015312.01.T01 | XP_013465092.1 | 85.714 | 70 | 9 | 1 | 1 | 69 | 1 | 70 | 2.11E-13 | 68.9 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380015312.01 | MsG0380016963.01 | 0.804408 | 2.191913e-49 | 1.129225e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015312.01.T01 | MTR_2g090340 | 85.714 | 70 | 9 | 1 | 1 | 69 | 1 | 70 | 2.55e-17 | 68.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 37 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTAATTTCATTACCCATTA+AGG | 0.186380 | 3:-68310379 | None:intergenic |
| AGAGAGAGACAGAGGAAATT+TGG | 0.190544 | 3:-68309752 | None:intergenic |
| AAATTAAAAGTATAAGATTT+TGG | 0.202391 | 3:+68310394 | MsG0380015312.01.T01:three_prime_UTR |
| GAAGAAGAAATTTCACTTGT+TGG | 0.262579 | 3:+68310267 | MsG0380015312.01.T01:three_prime_UTR |
| CGCTGTCTCCGTTTCTTCTT+CGG | 0.298620 | 3:+68309931 | MsG0380015312.01.T01:CDS |
| GGAAGCGGCGGCGACGGAGG+CGG | 0.308504 | 3:-68309903 | None:intergenic |
| TGAGAGAAACAAATGAAGAT+TGG | 0.330432 | 3:-68309847 | None:intergenic |
| GAGAGAAACAAATGAAGATT+GGG | 0.372997 | 3:-68309846 | None:intergenic |
| CGGCGACGGAGGCGGCGAAG+AGG | 0.418581 | 3:-68309895 | None:intergenic |
| GGAGACAGCGGTGGCGGAAG+CGG | 0.428305 | 3:-68309918 | None:intergenic |
| GGTGGCGGAAGCGGCGGCGA+CGG | 0.429714 | 3:-68309909 | None:intergenic |
| GGCAAATAACTCAAATCATC+AGG | 0.435276 | 3:+68309952 | MsG0380015312.01.T01:CDS |
| GAAGAAGAAGAGAGAGAAAG+AGG | 0.446300 | 3:-68309788 | None:intergenic |
| TCATAGATGAAAGAGGGGTT+TGG | 0.481099 | 3:+68310185 | MsG0380015312.01.T01:exon |
| GGCGGAAGCGGCGGCGACGG+AGG | 0.483294 | 3:-68309906 | None:intergenic |
| ACCGCCACAATGAAGAAATC+CGG | 0.492103 | 3:+68309872 | MsG0380015312.01.T01:exon |
| GACGGAGGCGGCGAAGAGGC+CGG | 0.501966 | 3:-68309891 | None:intergenic |
| GACAGCGGTGGCGGAAGCGG+CGG | 0.505444 | 3:-68309915 | None:intergenic |
| AGAAACGGAGACAGCGGTGG+CGG | 0.505992 | 3:-68309924 | None:intergenic |
| TGATACGCGAAATTATTACT+TGG | 0.513903 | 3:+68310338 | MsG0380015312.01.T01:three_prime_UTR |
| GTTATTTGCCGAAGAAGAAA+CGG | 0.515047 | 3:-68309939 | None:intergenic |
| GAAATTGCGCCGAGATTCGA+TGG | 0.516423 | 3:+68310132 | MsG0380015312.01.T01:CDS |
| AAACGCAAACCATCGAATCT+CGG | 0.521279 | 3:-68310141 | None:intergenic |
| AGACAGAGGAAATTTGGTTG+CGG | 0.546519 | 3:-68309746 | None:intergenic |
| AACTGCTCATAGATGAAAGA+GGG | 0.547718 | 3:+68310179 | MsG0380015312.01.T01:exon |
| ACAAGACGAAGAAACATATG+AGG | 0.552549 | 3:-68310222 | None:intergenic |
| ATTTGTAGAGAGTGAATATG+TGG | 0.567779 | 3:-68309812 | None:intergenic |
| GAGGCCGGATTTCTTCATTG+TGG | 0.578217 | 3:-68309876 | None:intergenic |
| GTCTTGTTGAACTTCTTGTG+TGG | 0.583419 | 3:+68310238 | MsG0380015312.01.T01:three_prime_UTR |
| GCCGGATTTCTTCATTGTGG+CGG | 0.585971 | 3:-68309873 | None:intergenic |
| TAACTGCTCATAGATGAAAG+AGG | 0.603127 | 3:+68310178 | MsG0380015312.01.T01:exon |
| GAGATCTTCATCGTCGACGA+CGG | 0.614743 | 3:+68310108 | MsG0380015312.01.T01:CDS |
| AGAAGAAACGGAGACAGCGG+TGG | 0.633424 | 3:-68309927 | None:intergenic |
| GACGGTACGGTTGAAAGAAG+AGG | 0.642636 | 3:+68310078 | MsG0380015312.01.T01:CDS |
| CGAAGAAGAAACGGAGACAG+CGG | 0.672061 | 3:-68309930 | None:intergenic |
| ACTGCTCATAGATGAAAGAG+GGG | 0.694445 | 3:+68310180 | MsG0380015312.01.T01:exon |
| CGACGATGAAGATCTCATCA+CGG | 0.700873 | 3:-68310101 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATTAAAAGTATAAGATTT+TGG | + | Chr3:68310394-68310413 | MsG0380015312.01.T01:three_prime_UTR | 10.0% |
| !!! | TGAATAAATTTTCAGATCTT+GGG | - | Chr3:68310008-68310027 | None:intergenic | 20.0% |
| !!! | TTCTTTTTGTAATCCTTAAT+GGG | + | Chr3:68310366-68310385 | MsG0380015312.01.T01:three_prime_UTR | 20.0% |
| !!! | TTGAATAAATTTTCAGATCT+TGG | - | Chr3:68310009-68310028 | None:intergenic | 20.0% |
| !!! | TTTAATTTCATTACCCATTA+AGG | - | Chr3:68310382-68310401 | None:intergenic | 20.0% |
| !!! | TTTCTTTTTGTAATCCTTAA+TGG | + | Chr3:68310365-68310384 | MsG0380015312.01.T01:three_prime_UTR | 20.0% |
| !! | AATTTGTGTTTTGTATAGGA+CGG | + | Chr3:68310060-68310079 | MsG0380015312.01.T01:intron | 25.0% |
| !! | TGTGAATTTGTGTTTTGTAT+AGG | + | Chr3:68310056-68310075 | MsG0380015312.01.T01:intron | 25.0% |
| ATTTGTAGAGAGTGAATATG+TGG | - | Chr3:68309815-68309834 | None:intergenic | 30.0% | |
| GAAGAAGAAATTTCACTTGT+TGG | + | Chr3:68310267-68310286 | MsG0380015312.01.T01:three_prime_UTR | 30.0% | |
| GAGAGAAACAAATGAAGATT+GGG | - | Chr3:68309849-68309868 | None:intergenic | 30.0% | |
| TGAGAGAAACAAATGAAGAT+TGG | - | Chr3:68309850-68309869 | None:intergenic | 30.0% | |
| TGATACGCGAAATTATTACT+TGG | + | Chr3:68310338-68310357 | MsG0380015312.01.T01:three_prime_UTR | 30.0% | |
| AACTGCTCATAGATGAAAGA+GGG | + | Chr3:68310179-68310198 | MsG0380015312.01.T01:exon | 35.0% | |
| ACAAGACGAAGAAACATATG+AGG | - | Chr3:68310225-68310244 | None:intergenic | 35.0% | |
| GGCAAATAACTCAAATCATC+AGG | + | Chr3:68309952-68309971 | MsG0380015312.01.T01:CDS | 35.0% | |
| GTTATTTGCCGAAGAAGAAA+CGG | - | Chr3:68309942-68309961 | None:intergenic | 35.0% | |
| TAACTGCTCATAGATGAAAG+AGG | + | Chr3:68310178-68310197 | MsG0380015312.01.T01:exon | 35.0% | |
| AAACGCAAACCATCGAATCT+CGG | - | Chr3:68310144-68310163 | None:intergenic | 40.0% | |
| ACTGCTCATAGATGAAAGAG+GGG | + | Chr3:68310180-68310199 | MsG0380015312.01.T01:exon | 40.0% | |
| AGACAGAGGAAATTTGGTTG+CGG | - | Chr3:68309749-68309768 | None:intergenic | 40.0% | |
| AGAGAGAGACAGAGGAAATT+TGG | - | Chr3:68309755-68309774 | None:intergenic | 40.0% | |
| GAAGAAGAAGAGAGAGAAAG+AGG | - | Chr3:68309791-68309810 | None:intergenic | 40.0% | |
| TCATAGATGAAAGAGGGGTT+TGG | + | Chr3:68310185-68310204 | MsG0380015312.01.T01:exon | 40.0% | |
| ! | GTCTTGTTGAACTTCTTGTG+TGG | + | Chr3:68310238-68310257 | MsG0380015312.01.T01:three_prime_UTR | 40.0% |
| ! | GTGTTTTGTATAGGACGGTA+CGG | + | Chr3:68310065-68310084 | MsG0380015312.01.T01:intron | 40.0% |
| ACCGCCACAATGAAGAAATC+CGG | + | Chr3:68309872-68309891 | MsG0380015312.01.T01:exon | 45.0% | |
| CGACGATGAAGATCTCATCA+CGG | - | Chr3:68310104-68310123 | None:intergenic | 45.0% | |
| AGAGAGAGAGAGAGAGACAG+AGG | - | Chr3:68309763-68309782 | None:intergenic | 50.0% | |
| CGAAGAAGAAACGGAGACAG+CGG | - | Chr3:68309933-68309952 | None:intergenic | 50.0% | |
| CGCTGTCTCCGTTTCTTCTT+CGG | + | Chr3:68309931-68309950 | MsG0380015312.01.T01:CDS | 50.0% | |
| GAAATTGCGCCGAGATTCGA+TGG | + | Chr3:68310132-68310151 | MsG0380015312.01.T01:CDS | 50.0% | |
| GACGGTACGGTTGAAAGAAG+AGG | + | Chr3:68310078-68310097 | MsG0380015312.01.T01:CDS | 50.0% | |
| GAGATCTTCATCGTCGACGA+CGG | + | Chr3:68310108-68310127 | MsG0380015312.01.T01:CDS | 50.0% | |
| GAGGCCGGATTTCTTCATTG+TGG | - | Chr3:68309879-68309898 | None:intergenic | 50.0% | |
| GCCGGATTTCTTCATTGTGG+CGG | - | Chr3:68309876-68309895 | None:intergenic | 50.0% | |
| AGAAGAAACGGAGACAGCGG+TGG | - | Chr3:68309930-68309949 | None:intergenic | 55.0% | |
| AGAAACGGAGACAGCGGTGG+CGG | - | Chr3:68309927-68309946 | None:intergenic | 60.0% | |
| !! | GGAGACAGCGGTGGCGGAAG+CGG | - | Chr3:68309921-68309940 | None:intergenic | 70.0% |
| GACGGAGGCGGCGAAGAGGC+CGG | - | Chr3:68309894-68309913 | None:intergenic | 75.0% | |
| !! | GACAGCGGTGGCGGAAGCGG+CGG | - | Chr3:68309918-68309937 | None:intergenic | 75.0% |
| ! | CGGCGACGGAGGCGGCGAAG+AGG | - | Chr3:68309898-68309917 | None:intergenic | 80.0% |
| ! | GGAAGCGGCGGCGACGGAGG+CGG | - | Chr3:68309906-68309925 | None:intergenic | 80.0% |
| !!! | GGTGGCGGAAGCGGCGGCGA+CGG | - | Chr3:68309912-68309931 | None:intergenic | 80.0% |
| ! | GGCGGAAGCGGCGGCGACGG+AGG | - | Chr3:68309909-68309928 | None:intergenic | 85.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 68309746 | 68310433 | 68309746 | ID=MsG0380015312.01;Name=MsG0380015312.01 |
| Chr3 | mRNA | 68309746 | 68310433 | 68309746 | ID=MsG0380015312.01.T01;Parent=MsG0380015312.01;Name=MsG0380015312.01.T01;_AED=0.49;_eAED=0.49;_QI=135|1|1|1|1|1|2|239|69 |
| Chr3 | exon | 68309746 | 68309973 | 68309746 | ID=MsG0380015312.01.T01:exon:13574;Parent=MsG0380015312.01.T01 |
| Chr3 | exon | 68310078 | 68310433 | 68310078 | ID=MsG0380015312.01.T01:exon:13575;Parent=MsG0380015312.01.T01 |
| Chr3 | five_prime_UTR | 68309746 | 68309880 | 68309746 | ID=MsG0380015312.01.T01:five_prime_utr;Parent=MsG0380015312.01.T01 |
| Chr3 | CDS | 68309881 | 68309973 | 68309881 | ID=MsG0380015312.01.T01:cds;Parent=MsG0380015312.01.T01 |
| Chr3 | CDS | 68310078 | 68310194 | 68310078 | ID=MsG0380015312.01.T01:cds;Parent=MsG0380015312.01.T01 |
| Chr3 | three_prime_UTR | 68310195 | 68310433 | 68310195 | ID=MsG0380015312.01.T01:three_prime_utr;Parent=MsG0380015312.01.T01 |
| Gene Sequence |
| Protein sequence |
