Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015663.01.T01 | XP_013460891.1 | 94.595 | 74 | 4 | 0 | 1 | 74 | 436 | 509 | 2.55E-41 | 151 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015663.01.T01 | Q93Y91 | 65.079 | 63 | 22 | 0 | 3 | 65 | 440 | 502 | 3.60E-24 | 96.7 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015663.01.T01 | A0A072V9T5 | 94.595 | 74 | 4 | 0 | 1 | 74 | 436 | 509 | 1.22e-41 | 151 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380015663.01 | MsG0780036147.01 | 0.812961 | 3.256662e-51 | 2.099317e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015663.01.T01 | MTR_3g074410 | 94.595 | 74 | 4 | 0 | 1 | 74 | 436 | 509 | 3.09e-45 | 151 |
| MsG0380015663.01.T01 | MTR_3g074340 | 84.722 | 72 | 11 | 0 | 3 | 74 | 269 | 340 | 2.03e-37 | 127 |
| MsG0380015663.01.T01 | MTR_3g074320 | 83.333 | 72 | 12 | 0 | 3 | 74 | 269 | 340 | 6.02e-37 | 126 |
| MsG0380015663.01.T01 | MTR_3g074370 | 84.722 | 72 | 11 | 0 | 3 | 74 | 269 | 340 | 2.40e-36 | 124 |
| MsG0380015663.01.T01 | MTR_3g074390 | 76.389 | 72 | 17 | 0 | 3 | 74 | 441 | 512 | 1.51e-33 | 119 |
| MsG0380015663.01.T01 | MTR_3g074270 | 70.312 | 64 | 19 | 0 | 3 | 66 | 415 | 478 | 7.36e-27 | 101 |
| MsG0380015663.01.T01 | MTR_0204s0020 | 61.290 | 62 | 24 | 0 | 3 | 64 | 428 | 489 | 1.11e-24 | 95.1 |
| MsG0380015663.01.T01 | MTR_0204s0020 | 61.290 | 62 | 24 | 0 | 3 | 64 | 431 | 492 | 1.12e-24 | 95.1 |
| MsG0380015663.01.T01 | MTR_4g116790 | 56.923 | 65 | 28 | 0 | 3 | 67 | 437 | 501 | 9.28e-24 | 92.8 |
| MsG0380015663.01.T01 | MTR_5g006070 | 53.731 | 67 | 30 | 1 | 3 | 68 | 388 | 454 | 9.82e-23 | 89.7 |
| MsG0380015663.01.T01 | MTR_5g006070 | 53.731 | 67 | 30 | 1 | 3 | 68 | 436 | 502 | 1.08e-22 | 89.7 |
| MsG0380015663.01.T01 | MTR_3g008160 | 53.521 | 71 | 32 | 1 | 3 | 73 | 387 | 456 | 1.75e-22 | 89.0 |
| MsG0380015663.01.T01 | MTR_3g008160 | 53.521 | 71 | 32 | 1 | 3 | 73 | 436 | 505 | 2.22e-22 | 89.0 |
| MsG0380015663.01.T01 | MTR_7g091690 | 62.069 | 58 | 22 | 0 | 3 | 60 | 437 | 494 | 4.34e-22 | 88.2 |
| MsG0380015663.01.T01 | MTR_3g008170 | 56.338 | 71 | 30 | 1 | 3 | 72 | 437 | 507 | 5.28e-22 | 87.8 |
| MsG0380015663.01.T01 | MTR_5g094760 | 62.069 | 58 | 22 | 0 | 3 | 60 | 437 | 494 | 6.97e-22 | 87.4 |
| MsG0380015663.01.T01 | MTR_4g116770 | 60.345 | 58 | 23 | 0 | 3 | 60 | 635 | 692 | 1.69e-20 | 83.6 |
| MsG0380015663.01.T01 | MTR_0204s0040 | 60.345 | 58 | 23 | 0 | 3 | 60 | 439 | 496 | 1.94e-20 | 83.6 |
| MsG0380015663.01.T01 | MTR_0009s0370 | 54.545 | 66 | 30 | 0 | 3 | 68 | 437 | 502 | 6.71e-20 | 82.0 |
| MsG0380015663.01.T01 | MTR_1g104750 | 56.452 | 62 | 26 | 1 | 3 | 63 | 436 | 497 | 1.33e-19 | 80.9 |
| MsG0380015663.01.T01 | MTR_3g007910 | 52.174 | 69 | 33 | 0 | 3 | 71 | 406 | 474 | 2.95e-18 | 77.4 |
| MsG0380015663.01.T01 | MTR_1g104770 | 53.226 | 62 | 28 | 1 | 3 | 63 | 437 | 498 | 4.25e-18 | 76.6 |
| MsG0380015663.01.T01 | MTR_8g102860 | 43.056 | 72 | 40 | 1 | 4 | 74 | 387 | 458 | 2.75e-17 | 74.3 |
| MsG0380015663.01.T01 | MTR_8g102860 | 43.056 | 72 | 40 | 1 | 4 | 74 | 439 | 510 | 3.96e-17 | 73.9 |
| MsG0380015663.01.T01 | MTR_6g087040 | 43.077 | 65 | 37 | 0 | 4 | 68 | 439 | 503 | 4.67e-16 | 70.9 |
| MsG0380015663.01.T01 | MTR_6g088375 | 43.077 | 65 | 37 | 0 | 4 | 68 | 439 | 503 | 4.67e-16 | 70.9 |
| MsG0380015663.01.T01 | MTR_5g082540 | 52.381 | 63 | 30 | 0 | 3 | 65 | 436 | 498 | 2.73e-15 | 68.9 |
| MsG0380015663.01.T01 | MTR_1g038630 | 51.389 | 72 | 34 | 1 | 3 | 73 | 436 | 507 | 5.57e-15 | 67.8 |
| MsG0380015663.01.T01 | MTR_1g038600 | 54.839 | 62 | 28 | 0 | 3 | 64 | 418 | 479 | 7.23e-15 | 67.8 |
| MsG0380015663.01.T01 | MTR_3g023480 | 53.030 | 66 | 31 | 0 | 3 | 68 | 437 | 502 | 2.79e-14 | 65.9 |
| MsG0380015663.01.T01 | MTR_1g104780 | 47.436 | 78 | 34 | 3 | 4 | 74 | 437 | 514 | 7.19e-14 | 64.7 |
| MsG0380015663.01.T01 | MTR_4g091370 | 54.286 | 70 | 31 | 1 | 3 | 71 | 438 | 507 | 1.04e-13 | 64.3 |
| MsG0380015663.01.T01 | MTR_5g041550 | 52.113 | 71 | 34 | 0 | 3 | 73 | 439 | 509 | 2.53e-13 | 63.2 |
| MsG0380015663.01.T01 | MTR_3g074400 | 44.681 | 94 | 10 | 2 | 1 | 74 | 353 | 424 | 4.73e-13 | 62.4 |
| MsG0380015663.01.T01 | MTR_3g093010 | 54.545 | 66 | 30 | 0 | 3 | 68 | 438 | 503 | 5.52e-13 | 62.4 |
| MsG0380015663.01.T01 | MTR_5g096000 | 50.000 | 70 | 35 | 0 | 3 | 72 | 237 | 306 | 1.19e-11 | 58.2 |
| MsG0380015663.01.T01 | MTR_1g114280 | 46.552 | 58 | 30 | 1 | 7 | 64 | 97 | 153 | 2.60e-11 | 55.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015663.01.T01 | AT1G34580 | 65.079 | 63 | 22 | 0 | 3 | 65 | 440 | 502 | 3.67e-25 | 96.7 |
| MsG0380015663.01.T01 | AT1G34580 | 65.079 | 63 | 22 | 0 | 3 | 65 | 390 | 452 | 3.68e-25 | 96.7 |
| MsG0380015663.01.T01 | AT4G02050 | 60.000 | 60 | 24 | 0 | 3 | 62 | 437 | 496 | 3.57e-22 | 88.6 |
| MsG0380015663.01.T01 | AT4G02050 | 60.000 | 60 | 24 | 0 | 3 | 62 | 437 | 496 | 3.57e-22 | 88.6 |
| MsG0380015663.01.T01 | AT5G26340 | 49.367 | 79 | 30 | 2 | 3 | 71 | 437 | 515 | 1.56e-21 | 86.7 |
| MsG0380015663.01.T01 | AT3G19940 | 53.731 | 67 | 30 | 1 | 3 | 69 | 438 | 503 | 1.89e-19 | 80.9 |
| MsG0380015663.01.T01 | AT5G26250 | 52.239 | 67 | 31 | 1 | 3 | 68 | 432 | 498 | 2.24e-19 | 80.5 |
| MsG0380015663.01.T01 | AT3G05960 | 52.239 | 67 | 31 | 1 | 3 | 68 | 431 | 497 | 2.24e-19 | 80.5 |
| MsG0380015663.01.T01 | AT1G50310 | 53.846 | 65 | 30 | 0 | 3 | 67 | 296 | 360 | 7.80e-19 | 78.6 |
| MsG0380015663.01.T01 | AT1G50310 | 53.846 | 65 | 30 | 0 | 3 | 67 | 439 | 503 | 1.05e-18 | 78.6 |
| MsG0380015663.01.T01 | AT1G11260 | 60.563 | 71 | 25 | 2 | 3 | 72 | 438 | 506 | 4.40e-18 | 76.6 |
| MsG0380015663.01.T01 | AT5G61520 | 46.970 | 66 | 35 | 0 | 3 | 68 | 392 | 457 | 3.83e-17 | 73.9 |
| MsG0380015663.01.T01 | AT5G61520 | 46.970 | 66 | 35 | 0 | 3 | 68 | 440 | 505 | 4.06e-17 | 73.9 |
| MsG0380015663.01.T01 | AT5G23270 | 55.556 | 54 | 24 | 0 | 10 | 63 | 446 | 499 | 4.19e-16 | 71.2 |
| MsG0380015663.01.T01 | AT1G77210 | 41.538 | 65 | 38 | 0 | 4 | 68 | 290 | 354 | 7.01e-16 | 70.5 |
| MsG0380015663.01.T01 | AT1G77210 | 41.538 | 65 | 38 | 0 | 4 | 68 | 439 | 503 | 1.39e-15 | 69.7 |
| MsG0380015663.01.T01 | AT1G77210 | 41.538 | 65 | 38 | 0 | 4 | 68 | 439 | 503 | 1.39e-15 | 69.7 |
| MsG0380015663.01.T01 | AT3G19930 | 53.846 | 65 | 30 | 0 | 3 | 67 | 436 | 500 | 2.24e-15 | 68.9 |
| MsG0380015663.01.T01 | AT3G19930 | 53.846 | 65 | 30 | 0 | 3 | 67 | 436 | 500 | 2.24e-15 | 68.9 |
| MsG0380015663.01.T01 | AT4G21480 | 50.769 | 65 | 32 | 0 | 3 | 67 | 436 | 500 | 1.99e-13 | 63.5 |
Find 13 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATGGGGTAAACATTGGTTT+TGG | 0.345672 | 3:+73280632 | MsG0380015663.01.T01:CDS |
| ATGTTATGCCACTTTAAGTT+CGG | 0.376560 | 3:+73280508 | MsG0380015663.01.T01:CDS |
| TGTTATGCCACTTTAAGTTC+GGG | 0.386010 | 3:+73280509 | MsG0380015663.01.T01:CDS |
| TGGAGTCAATGCACATCATA+TGG | 0.390532 | 3:+73280614 | MsG0380015663.01.T01:CDS |
| CGGTTTGTTAAAGGAGAAGA+TGG | 0.426425 | 3:+73280658 | MsG0380015663.01.T01:CDS |
| GGAGACTAAAGGAATTGCTT+TGG | 0.447213 | 3:+73280594 | MsG0380015663.01.T01:CDS |
| GGAGTCAATGCACATCATAT+GGG | 0.483226 | 3:+73280615 | MsG0380015663.01.T01:CDS |
| AAAGCAATTCCTTTAGTCTC+CGG | 0.505065 | 3:-73280592 | None:intergenic |
| AAAATGCCCCGAACTTAAAG+TGG | 0.523444 | 3:-73280516 | None:intergenic |
| GTTAAAGGAGAAGATGGAGA+AGG | 0.565819 | 3:+73280664 | MsG0380015663.01.T01:CDS |
| ACATCATATGGGGTAAACAT+TGG | 0.571808 | 3:+73280626 | MsG0380015663.01.T01:CDS |
| GTTATGCCACTTTAAGTTCG+GGG | 0.589545 | 3:+73280510 | MsG0380015663.01.T01:CDS |
| GAGTCAATGCACATCATATG+GGG | 0.640900 | 3:+73280616 | MsG0380015663.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTCATTATATTTTTCTTGC+CGG | + | Chr3:73280573-73280592 | MsG0380015663.01.T01:CDS | 20.0% |
| !! | ATGTTATGCCACTTTAAGTT+CGG | + | Chr3:73280508-73280527 | MsG0380015663.01.T01:CDS | 30.0% |
| !! | ATTTTTGTTCTATGCTGGTT+GGG | + | Chr3:73280534-73280553 | MsG0380015663.01.T01:CDS | 30.0% |
| !!! | GTAAACATTGGTTTTGGTAT+CGG | + | Chr3:73280638-73280657 | MsG0380015663.01.T01:CDS | 30.0% |
| !!! | TTTTGGTATCGGTTTGTTAA+AGG | + | Chr3:73280649-73280668 | MsG0380015663.01.T01:CDS | 30.0% |
| AAAGCAATTCCTTTAGTCTC+CGG | - | Chr3:73280595-73280614 | None:intergenic | 35.0% | |
| ACATCATATGGGGTAAACAT+TGG | + | Chr3:73280626-73280645 | MsG0380015663.01.T01:CDS | 35.0% | |
| ! | CATTTTTGTTCTATGCTGGT+TGG | + | Chr3:73280533-73280552 | MsG0380015663.01.T01:CDS | 35.0% |
| !! | TGTTATGCCACTTTAAGTTC+GGG | + | Chr3:73280509-73280528 | MsG0380015663.01.T01:CDS | 35.0% |
| !!! | TATGGGGTAAACATTGGTTT+TGG | + | Chr3:73280632-73280651 | MsG0380015663.01.T01:CDS | 35.0% |
| AAAATGCCCCGAACTTAAAG+TGG | - | Chr3:73280519-73280538 | None:intergenic | 40.0% | |
| GAGTCAATGCACATCATATG+GGG | + | Chr3:73280616-73280635 | MsG0380015663.01.T01:CDS | 40.0% | |
| GGAGACTAAAGGAATTGCTT+TGG | + | Chr3:73280594-73280613 | MsG0380015663.01.T01:CDS | 40.0% | |
| GGAGTCAATGCACATCATAT+GGG | + | Chr3:73280615-73280634 | MsG0380015663.01.T01:CDS | 40.0% | |
| GTTAAAGGAGAAGATGGAGA+AGG | + | Chr3:73280664-73280683 | MsG0380015663.01.T01:CDS | 40.0% | |
| TGGAGTCAATGCACATCATA+TGG | + | Chr3:73280614-73280633 | MsG0380015663.01.T01:CDS | 40.0% | |
| ! | TTTTTCTTGCCGGAGACTAA+AGG | + | Chr3:73280583-73280602 | MsG0380015663.01.T01:CDS | 40.0% |
| !! | CGGTTTGTTAAAGGAGAAGA+TGG | + | Chr3:73280658-73280677 | MsG0380015663.01.T01:CDS | 40.0% |
| !! | GTTATGCCACTTTAAGTTCG+GGG | + | Chr3:73280510-73280529 | MsG0380015663.01.T01:CDS | 40.0% |
| !! | GGGGCATTTTTGTTCTATGC+TGG | + | Chr3:73280529-73280548 | MsG0380015663.01.T01:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 73280475 | 73280699 | 73280475 | ID=MsG0380015663.01;Name=MsG0380015663.01 |
| Chr3 | mRNA | 73280475 | 73280699 | 73280475 | ID=MsG0380015663.01.T01;Parent=MsG0380015663.01;Name=MsG0380015663.01.T01;_AED=0.40;_eAED=0.40;_QI=0|-1|0|1|-1|1|1|0|74 |
| Chr3 | exon | 73280475 | 73280699 | 73280475 | ID=MsG0380015663.01.T01:exon:14124;Parent=MsG0380015663.01.T01 |
| Chr3 | CDS | 73280475 | 73280699 | 73280475 | ID=MsG0380015663.01.T01:cds;Parent=MsG0380015663.01.T01 |
| Gene Sequence |
| Protein sequence |
