AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380016128.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016128.01.T01 MTR_3g083910 96.899 129 4 0 1 129 1 129 1.57e-88 263
MsG0380016128.01.T01 MTR_3g083920 90.698 129 12 0 1 129 1 129 4.46e-84 251
MsG0380016128.01.T01 MTR_5g007760 91.200 125 11 0 1 125 1 125 1.07e-81 246
MsG0380016128.01.T01 MTR_7g016780 90.400 125 12 0 1 125 1 125 1.59e-81 245
MsG0380016128.01.T01 MTR_1g097935 90.400 125 12 0 1 125 1 125 1.66e-81 245
MsG0380016128.01.T01 MTR_7g016720 90.400 125 12 0 1 125 1 125 1.70e-81 245
MsG0380016128.01.T01 MTR_1g097910 90.400 125 12 0 1 125 1 125 1.70e-81 245
MsG0380016128.01.T01 MTR_7g016800 90.400 125 12 0 1 125 1 125 1.94e-81 245
MsG0380016128.01.T01 MTR_1g098140 90.400 125 12 0 1 125 1 125 2.07e-81 245
MsG0380016128.01.T01 MTR_5g007730 89.600 125 13 0 1 125 1 125 4.57e-81 244
MsG0380016128.01.T01 MTR_5g007717 87.597 129 16 0 1 129 1 129 4.93e-81 244
MsG0380016128.01.T01 MTR_7g016700 89.600 125 13 0 1 125 1 125 7.53e-81 243
MsG0380016128.01.T01 MTR_7g016820 89.600 125 13 0 1 125 1 125 9.25e-81 243
MsG0380016128.01.T01 MTR_5g007713 86.822 129 17 0 1 129 1 129 1.02e-80 243
MsG0380016128.01.T01 MTR_3g086260 85.271 129 19 0 1 129 1 129 1.87e-78 237
MsG0380016128.01.T01 MTR_2g058470 80.620 129 25 0 1 129 1 129 5.78e-75 228
MsG0380016128.01.T01 MTR_7g084300 77.519 129 29 0 1 129 1 129 2.32e-72 222
MsG0380016128.01.T01 MTR_3g088675 63.566 129 47 0 1 129 1 129 1.02e-56 182
MsG0380016128.01.T01 MTR_5g007723 55.738 122 33 3 12 114 32 151 7.85e-36 127
MsG0380016128.01.T01 MTR_5g007770 83.333 60 10 0 70 129 1 60 6.89e-29 107
MsG0380016128.01.T01 MTR_5g007740 71.212 66 19 0 64 129 1 66 6.18e-27 102
MsG0380016128.01.T01 MTR_3g088670 44.915 118 60 2 10 125 2 116 2.13e-24 97.1
MsG0380016128.01.T01 MTR_7g113405 97.436 39 1 0 87 125 1 39 1.01e-18 80.5
MsG0380016128.01.T01 MTR_4g075560 38.261 115 70 1 11 125 13 126 1.05e-16 75.9
MsG0380016128.01.T01 MTR_4g078730 31.532 111 75 1 15 125 24 133 2.70e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016128.01.T01 AT5G13930 77.953 127 28 0 3 129 8 134 1.21e-70 218
MsG0380016128.01.T01 AT4G34850 36.937 111 69 1 15 125 18 127 1.40e-15 72.8
MsG0380016128.01.T01 AT1G02050 34.234 111 72 1 15 125 24 133 5.42e-14 68.2
MsG0380016128.01.T01 AT4G00040 32.432 111 74 1 15 125 17 126 1.85e-13 66.6

Find 59 sgRNAs with CRISPR-Local

Find 67 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATACACTTGGATTCTCTTTC+AGG 0.214226 3:+79411643 None:intergenic
CTTTCTGAGTCCTTGTTTAG+TGG 0.300015 3:-79412133 MsG0380016128.01.T01:five_prime_UTR
GGCCGTCAGCCCTCTGTGCC+TGG 0.321278 3:+79411982 None:intergenic
TTAGACTCAAAGAAAGAAAA+TGG 0.327165 3:-79412024 MsG0380016128.01.T01:exon
CGAGTACATGGCACCTTCAT+TGG 0.339316 3:-79411619 MsG0380016128.01.T01:CDS
CCTGCCACCGTGTTGGCAAT+CGG 0.362122 3:-79411960 MsG0380016128.01.T01:CDS
TATCAGCTCACAAAGCTCTT+AGG 0.381097 3:-79411448 None:intergenic
GAGGTGGTAATCTTGGACTT+AGG 0.389841 3:+79411513 None:intergenic
CCGATTGCCAACACGGTGGC+AGG 0.420233 3:+79411960 None:intergenic
AGGTTGACCCCATTCCTTGA+TGG 0.421592 3:+79411533 None:intergenic
AACAAAGGCCATCAAGGAAT+GGG 0.426157 3:-79411541 MsG0380016128.01.T01:CDS
CTGATAGGTTATATCTATCA+AGG 0.432913 3:+79412076 None:intergenic
CTGCCACCGTGTTGGCAATC+GGG 0.437033 3:-79411959 MsG0380016128.01.T01:CDS
TCTTTGAGTCTAAAGTTGGC+AGG 0.462540 3:+79412034 None:intergenic
AAGAAAAGGTGAGTACTGAT+AGG 0.462698 3:+79412061 None:intergenic
TCTTTCTTTGAGTCTAAAGT+TGG 0.473399 3:+79412030 None:intergenic
GTGATGCGGAAATAGTAGTC+AGG 0.474845 3:+79411897 None:intergenic
AAAAGATGAGGTGGTAATCT+TGG 0.479796 3:+79411506 None:intergenic
CAACAAAGGCCATCAAGGAA+TGG 0.481618 3:-79411542 MsG0380016128.01.T01:CDS
AGTAGTCAGGGTATGTGCTC+TGG 0.502001 3:+79411910 None:intergenic
ACCGGGCATGTCCACACCAC+TGG 0.508071 3:+79411477 None:intergenic
GCCATGTACTCGCATACACT+TGG 0.509273 3:+79411630 None:intergenic
TCCAAGTGTATGCGAGTACA+TGG 0.512057 3:-79411631 MsG0380016128.01.T01:CDS
AGTTGGCAGGGAGGAAGAAA+AGG 0.518931 3:+79412047 None:intergenic
CGCCAGGCACAGAGGGCTGA+CGG 0.520967 3:-79411984 MsG0380016128.01.T01:CDS
GGCAGCAACAAAGGCCATCA+AGG 0.527983 3:-79411547 MsG0380016128.01.T01:CDS
TGTGAGCTGATAGTCGGCAC+CGG 0.530104 3:+79411459 None:intergenic
AGGAAAAGAGGCAGCAACAA+AGG 0.538894 3:-79411556 MsG0380016128.01.T01:CDS
TGCAGTCCCGATTGCCAACA+CGG 0.542887 3:+79411953 None:intergenic
TGACGGCCCTGCCACCGTGT+TGG 0.545438 3:-79411967 MsG0380016128.01.T01:CDS
GGACTCAGAAAGAAAGCAAA+GGG 0.547479 3:+79412144 None:intergenic
GGTTATATCTATCAAGGGAT+AGG 0.549904 3:+79412082 None:intergenic
AGTACCAAGACTAGGAAAAG+AGG 0.550895 3:-79411568 MsG0380016128.01.T01:CDS
AAGACAAGACATGGTGGTTG+TGG 0.550960 3:-79411592 MsG0380016128.01.T01:CDS
TCTTGTCTTGCATCCAATGA+AGG 0.557589 3:+79411606 None:intergenic
CTTTGAGTCTAAAGTTGGCA+GGG 0.559117 3:+79412035 None:intergenic
TGAGATCCGCCAGGCACAGA+GGG 0.572520 3:-79411991 MsG0380016128.01.T01:CDS
GACAGTTGATGAGATCCGCC+AGG 0.581606 3:-79412000 MsG0380016128.01.T01:CDS
ACCAGTGGTGTGGACATGCC+CGG 0.582542 3:-79411478 MsG0380016128.01.T01:CDS
GTGAGCTGATAGTCGGCACC+GGG 0.590423 3:+79411460 None:intergenic
GAGCTTTGTGAGCTGATAGT+CGG 0.596466 3:+79411453 None:intergenic
TATGTTCACTGTTTGTGATG+CGG 0.599298 3:+79411883 None:intergenic
CGATTGCCAACACGGTGGCA+GGG 0.606331 3:+79411961 None:intergenic
GGGCATGTCCACACCACTGG+TGG 0.610981 3:+79411480 None:intergenic
TGATGCGGAAATAGTAGTCA+GGG 0.620998 3:+79411898 None:intergenic
AGGACTCAGAAAGAAAGCAA+AGG 0.635121 3:+79412143 None:intergenic
CGTCAGCCCTCTGTGCCTGG+CGG 0.639933 3:+79411985 None:intergenic
TGATAGGTTATATCTATCAA+GGG 0.640343 3:+79412077 None:intergenic
ATTGGATGCAAGACAAGACA+TGG 0.641833 3:-79411601 MsG0380016128.01.T01:CDS
TATCTATCAAGGGATAGGAA+TGG 0.644835 3:+79412087 None:intergenic
ACAAAGGCCATCAAGGAATG+GGG 0.662909 3:-79411540 MsG0380016128.01.T01:CDS
GTTGTGGAAGTACCAAGACT+AGG 0.667192 3:-79411576 MsG0380016128.01.T01:CDS
TGAGTCTAAAGTTGGCAGGG+AGG 0.671507 3:+79412038 None:intergenic
TGCAACTCCTCAAAACTGTG+TGG 0.671975 3:-79411934 MsG0380016128.01.T01:CDS
CACTGGTGGTGCAAAAGATG+AGG 0.677073 3:+79411494 None:intergenic
ATGAGATCCGCCAGGCACAG+AGG 0.679000 3:-79411992 MsG0380016128.01.T01:CDS
TGGTGGTGCAAAAGATGAGG+TGG 0.689658 3:+79411497 None:intergenic
GGATGCAAGACAAGACATGG+TGG 0.788462 3:-79411598 MsG0380016128.01.T01:CDS
AGTCCCGATTGCCAACACGG+TGG 0.788994 3:+79411956 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TGATAGGTTATATCTATCAA+GGG + Chr3:79411525-79411544 None:intergenic 25.0%
! TTAGACTCAAAGAAAGAAAA+TGG - Chr3:79411575-79411594 MsG0380016128.01.T01:CDS 25.0%
!!! TAAACTGCATATACTTTTTC+AGG - Chr3:79411885-79411904 MsG0380016128.01.T01:CDS 25.0%
AGAGTGAAAGATCAAATTTG+AGG + Chr3:79411842-79411861 None:intergenic 30.0%
CTGATAGGTTATATCTATCA+AGG + Chr3:79411526-79411545 None:intergenic 30.0%
! TCTTTCTTTGAGTCTAAAGT+TGG + Chr3:79411572-79411591 None:intergenic 30.0%
!!! AAGTTTTTTGCCACTAAACA+AGG + Chr3:79411479-79411498 None:intergenic 30.0%
AAAAGATGAGGTGGTAATCT+TGG + Chr3:79412096-79412115 None:intergenic 35.0%
ATACACTTGGATTCTCTTTC+AGG + Chr3:79411959-79411978 None:intergenic 35.0%
GGTTATATCTATCAAGGGAT+AGG + Chr3:79411520-79411539 None:intergenic 35.0%
TATCTATCAAGGGATAGGAA+TGG + Chr3:79411515-79411534 None:intergenic 35.0%
TATGTTCACTGTTTGTGATG+CGG + Chr3:79411719-79411738 None:intergenic 35.0%
!! AAGAAAAGGTGAGTACTGAT+AGG + Chr3:79411541-79411560 None:intergenic 35.0%
AACAAAGGCCATCAAGGAAT+GGG - Chr3:79412058-79412077 MsG0380016128.01.T01:five_prime_UTR 40.0%
AGAGCACGGAAAGATATGTA+TGG + Chr3:79411793-79411812 None:intergenic 40.0%
AGGACTCAGAAAGAAAGCAA+AGG + Chr3:79411459-79411478 None:intergenic 40.0%
AGTACCAAGACTAGGAAAAG+AGG - Chr3:79412031-79412050 MsG0380016128.01.T01:five_prime_UTR 40.0%
ATTGGATGCAAGACAAGACA+TGG - Chr3:79411998-79412017 MsG0380016128.01.T01:CDS 40.0%
GGACTCAGAAAGAAAGCAAA+GGG + Chr3:79411458-79411477 None:intergenic 40.0%
TCTTGTCTTGCATCCAATGA+AGG + Chr3:79411996-79412015 None:intergenic 40.0%
TGATGCGGAAATAGTAGTCA+GGG + Chr3:79411704-79411723 None:intergenic 40.0%
! CTTTCTGAGTCCTTGTTTAG+TGG - Chr3:79411466-79411485 MsG0380016128.01.T01:CDS 40.0%
! CTTTGAGTCTAAAGTTGGCA+GGG + Chr3:79411567-79411586 None:intergenic 40.0%
! TCTTTGAGTCTAAAGTTGGC+AGG + Chr3:79411568-79411587 None:intergenic 40.0%
AAGACAAGACATGGTGGTTG+TGG - Chr3:79412007-79412026 MsG0380016128.01.T01:CDS 45.0%
ACAAAGGCCATCAAGGAATG+GGG - Chr3:79412059-79412078 MsG0380016128.01.T01:five_prime_UTR 45.0%
AGGAAAAGAGGCAGCAACAA+AGG - Chr3:79412043-79412062 MsG0380016128.01.T01:five_prime_UTR 45.0%
CAACAAAGGCCATCAAGGAA+TGG - Chr3:79412057-79412076 MsG0380016128.01.T01:five_prime_UTR 45.0%
CAATGTACAAGCACAGAGCA+CGG + Chr3:79411807-79411826 None:intergenic 45.0%
GAGGTGGTAATCTTGGACTT+AGG + Chr3:79412089-79412108 None:intergenic 45.0%
GTGATGCGGAAATAGTAGTC+AGG + Chr3:79411705-79411724 None:intergenic 45.0%
TCCAAGTGTATGCGAGTACA+TGG - Chr3:79411968-79411987 MsG0380016128.01.T01:CDS 45.0%
TGCAACTCCTCAAAACTGTG+TGG - Chr3:79411665-79411684 MsG0380016128.01.T01:CDS 45.0%
! GAGCTTTGTGAGCTGATAGT+CGG + Chr3:79412149-79412168 None:intergenic 45.0%
!! GTTGTGGAAGTACCAAGACT+AGG - Chr3:79412023-79412042 MsG0380016128.01.T01:exon 45.0%
AGGTTGACCCCATTCCTTGA+TGG + Chr3:79412069-79412088 None:intergenic 50.0%
AGTAGTCAGGGTATGTGCTC+TGG + Chr3:79411692-79411711 None:intergenic 50.0%
AGTTGGCAGGGAGGAAGAAA+AGG + Chr3:79411555-79411574 None:intergenic 50.0%
CGAGTACATGGCACCTTCAT+TGG - Chr3:79411980-79411999 MsG0380016128.01.T01:CDS 50.0%
GCCATGTACTCGCATACACT+TGG + Chr3:79411972-79411991 None:intergenic 50.0%
GGATGCAAGACAAGACATGG+TGG - Chr3:79412001-79412020 MsG0380016128.01.T01:CDS 50.0%
! CACTGGTGGTGCAAAAGATG+AGG + Chr3:79412108-79412127 None:intergenic 50.0%
! CTCATCTTTTGCACCACCAG+TGG - Chr3:79412106-79412125 MsG0380016128.01.T01:five_prime_UTR 50.0%
! CTCTGGTCCACACAGTTTTG+AGG + Chr3:79411675-79411694 None:intergenic 50.0%
! GCTGCCTCTTTTCCTAGTCT+TGG + Chr3:79412038-79412057 None:intergenic 50.0%
! TGAGTCTAAAGTTGGCAGGG+AGG + Chr3:79411564-79411583 None:intergenic 50.0%
! TGGTGGTGCAAAAGATGAGG+TGG + Chr3:79412105-79412124 None:intergenic 50.0%
GGCAGCAACAAAGGCCATCA+AGG - Chr3:79412052-79412071 MsG0380016128.01.T01:five_prime_UTR 55.0%
TGCAGTCCCGATTGCCAACA+CGG + Chr3:79411649-79411668 None:intergenic 55.0%
TGTGAGCTGATAGTCGGCAC+CGG + Chr3:79412143-79412162 None:intergenic 55.0%
! CTTTTGCACCACCAGTGGTG+TGG - Chr3:79412111-79412130 MsG0380016128.01.T01:five_prime_UTR 55.0%
!! GACAGTTGATGAGATCCGCC+AGG - Chr3:79411599-79411618 MsG0380016128.01.T01:CDS 55.0%
ACCAGTGGTGTGGACATGCC+CGG - Chr3:79412121-79412140 MsG0380016128.01.T01:five_prime_UTR 60.0%
AGTCCCGATTGCCAACACGG+TGG + Chr3:79411646-79411665 None:intergenic 60.0%
ATGAGATCCGCCAGGCACAG+AGG - Chr3:79411607-79411626 MsG0380016128.01.T01:CDS 60.0%
CGATTGCCAACACGGTGGCA+GGG + Chr3:79411641-79411660 None:intergenic 60.0%
GTGAGCTGATAGTCGGCACC+GGG + Chr3:79412142-79412161 None:intergenic 60.0%
TGAGATCCGCCAGGCACAGA+GGG - Chr3:79411608-79411627 MsG0380016128.01.T01:CDS 60.0%
! CCTGCCACCGTGTTGGCAAT+CGG - Chr3:79411639-79411658 MsG0380016128.01.T01:CDS 60.0%
! CTGCCACCGTGTTGGCAATC+GGG - Chr3:79411640-79411659 MsG0380016128.01.T01:CDS 60.0%
ACCGGGCATGTCCACACCAC+TGG + Chr3:79412125-79412144 None:intergenic 65.0%
CCGATTGCCAACACGGTGGC+AGG + Chr3:79411642-79411661 None:intergenic 65.0%
GGGCATGTCCACACCACTGG+TGG + Chr3:79412122-79412141 None:intergenic 65.0%
CGCCAGGCACAGAGGGCTGA+CGG - Chr3:79411615-79411634 MsG0380016128.01.T01:CDS 70.0%
CGTCAGCCCTCTGTGCCTGG+CGG + Chr3:79411617-79411636 None:intergenic 70.0%
TGACGGCCCTGCCACCGTGT+TGG - Chr3:79411632-79411651 MsG0380016128.01.T01:CDS 70.0%
GGCCGTCAGCCCTCTGTGCC+TGG + Chr3:79411620-79411639 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
Chr3 gene 79411449 79412172 79411449 ID=MsG0380016128.01;Name=MsG0380016128.01
Chr3 mRNA 79411449 79412172 79411449 ID=MsG0380016128.01.T01;Parent=MsG0380016128.01;Name=MsG0380016128.01.T01;_AED=0.39;_eAED=0.39;_QI=145|1|1|1|1|1|2|0|147
Chr3 exon 79411449 79411714 79411449 ID=MsG0380016128.01.T01:exon:18009;Parent=MsG0380016128.01.T01
Chr3 exon 79411850 79412172 79411850 ID=MsG0380016128.01.T01:exon:18008;Parent=MsG0380016128.01.T01
Chr3 five_prime_UTR 79412028 79412172 79412028 ID=MsG0380016128.01.T01:five_prime_utr;Parent=MsG0380016128.01.T01
Chr3 CDS 79411850 79412027 79411850 ID=MsG0380016128.01.T01:cds;Parent=MsG0380016128.01.T01
Chr3 CDS 79411449 79411714 79411449 ID=MsG0380016128.01.T01:cds;Parent=MsG0380016128.01.T01
Gene Sequence

>MsG0380016128.01.T01

ATGGTGACAGTTGATGAGATCCGCCAGGCACAGAGGGCTGACGGCCCTGCCACCGTGTTGGCAATCGGGACTGCAACTCCTCAAAACTGTGTGGACCAGAGCACATACCCTGACTACTATTTCCGCATCACAAACAGTGAACATAAGACTGAGCTCAAAGAAAAATTTCAGCGCATGTGTGATAAGTCGATGATCAAGAAGAGATACATGTACTTGACAGAAGAGATCCTGAAAGAGAATCCAAGTGTATGCGAGTACATGGCACCTTCATTGGATGCAAGACAAGACATGGTGGTTGTGGAAGTACCAAGACTAGGAAAAGAGGCAGCAACAAAGGCCATCAAGGAATGGGGTCAACCTAAGTCCAAGATTACCACCTCATCTTTTGCACCACCAGTGGTGTGGACATGCCCGGTGCCGACTATCAGCTCACAAAGCTCTTAG

Protein sequence

>MsG0380016128.01.T01

MVTVDEIRQAQRADGPATVLAIGTATPQNCVDQSTYPDYYFRITNSEHKTELKEKFQRMCDKSMIKKRYMYLTEEILKENPSVCEYMAPSLDARQDMVVVEVPRLGKEAATKAIKEWGQPKSKITTSSFAPPVVWTCPVPTISSQSS*