AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380016373.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016373.01.T01 MTR_3g088510 69.602 352 17 2 1 262 1 352 1.29e-153 436
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016373.01.T01 AT1G21710 50.519 289 92 6 7 250 11 293 1.22e-85 263

Find 111 sgRNAs with CRISPR-Local

Find 213 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAGTGGCGGGTTTGGGTTT+GGG 0.225556 3:+82623312 None:intergenic
TGGAGTGGCGGGTTTGGGTT+TGG 0.232265 3:+82623311 None:intergenic
CGGGTTTGGGTTTGGGGTGT+TGG 0.241345 3:+82623319 None:intergenic
GAGTGGCGGGTTTGGGTTTG+GGG 0.249611 3:+82623313 None:intergenic
GTTGGTGGAGTGGCGGGTTT+GGG 0.259029 3:+82623306 None:intergenic
GGTTGGTGGAGTGGCGGGTT+TGG 0.265429 3:+82623305 None:intergenic
CGATGAAAGATTTGCGGAAC+TGG 0.268100 3:-82622996 MsG0380016373.01.T01:CDS
TGGGGTGTTGGTGGTGTTGA+AGG 0.287882 3:+82623331 None:intergenic
GTTTGGGTTTGGGGTGTTGG+TGG 0.298537 3:+82623322 None:intergenic
GCTATACAAGCAGCCACTTT+AGG 0.318798 3:+82622521 None:intergenic
AGCCATTCTTCCCCACCTTC+AGG 0.363722 3:+82622611 None:intergenic
ATCTACGGGTTGTGAGTCTA+AGG 0.373502 3:-82621403 MsG0380016373.01.T01:CDS
ACCTGTGTCAATTGAAGGTT+AGG 0.380392 3:+82621201 None:intergenic
AATCATCGTTGGAGGGAGAT+TGG 0.385553 3:+82623092 None:intergenic
CGCCAAACATGAACATCAAC+AGG 0.405155 3:+82622467 None:intergenic
TTCCTGTTGATGTTCATGTT+TGG 0.414571 3:-82622469 MsG0380016373.01.T01:CDS
GATAGGGCTAAATATATAAC+TGG 0.417698 3:-82622659 MsG0380016373.01.T01:intron
TGAGAGGTTGGTGGAGTGGC+GGG 0.426079 3:+82623300 None:intergenic
TGCTGAGTTGCCTTCACAAA+AGG 0.426910 3:-82621609 MsG0380016373.01.T01:intron
GGTGTTGGTGGTGTTGAAGG+TGG 0.428047 3:+82623334 None:intergenic
TATCCACCTGTGTCAATTGA+AGG 0.434427 3:+82621196 None:intergenic
TCTCAATTCACCGGCGTCGT+TGG 0.434809 3:-82623175 MsG0380016373.01.T01:CDS
AGGACCAACTCCAGGTAACT+TGG 0.437622 3:+82622541 None:intergenic
AGCCTGAAGGTGGGGAAGAA+TGG 0.438759 3:-82622613 MsG0380016373.01.T01:CDS
TGACACGTGGAAGGTGTTCT+CGG 0.441693 3:-82623023 MsG0380016373.01.T01:CDS
ATCATCGTTGGAGGGAGATT+GGG 0.447502 3:+82623093 None:intergenic
CGCAACATTTGAGTGGTGCT+AGG 0.448095 3:-82622973 MsG0380016373.01.T01:CDS
ACCTCTCAAAATCCCGGGTA+TGG 0.449896 3:-82623285 MsG0380016373.01.T01:CDS
GTTGAAGGTGGAAGAGGAGG+AGG 0.456305 3:+82623346 None:intergenic
CCTCCTACCTTTACACTTGA+GGG 0.458910 3:-82621585 MsG0380016373.01.T01:CDS
TTGCTTGATTTCTTAAACGC+CGG 0.472258 3:-82623058 MsG0380016373.01.T01:CDS
AGGAGGAGGTAGTGATTTGG+TGG 0.473290 3:+82623360 None:intergenic
ACATGGAAGATGAACTTTGT+TGG 0.476317 3:-82621098 MsG0380016373.01.T01:CDS
AAGAGGAGGAGGTAGTGATT+TGG 0.478738 3:+82623357 None:intergenic
AGGTAAGGATAGTTCTTGTC+GGG 0.479365 3:+82623246 None:intergenic
TCAATTAGAGTGAGATCTAC+GGG 0.481116 3:-82621417 MsG0380016373.01.T01:intron
GGTGGTGTTGAAGGTGGAAG+AGG 0.481251 3:+82623340 None:intergenic
CAGCGGAAGGTTTGACCGGT+GGG 0.483622 3:+82623214 None:intergenic
GAAGGTTTGACCGGTGGGGA+AGG 0.484925 3:+82623219 None:intergenic
TGGAGGGAGATTGGGAGTGA+AGG 0.485854 3:+82623101 None:intergenic
CTTTGCGGGATTCTTCTGCT+TGG 0.488513 3:+82621559 None:intergenic
AACTGAATCAAGCATTCAAA+GGG 0.495318 3:+82622935 None:intergenic
GCTTTATTCTCTTCGCACAT+TGG 0.497338 3:-82622591 MsG0380016373.01.T01:CDS
TTGAGAGGTTGGTGGAGTGG+CGG 0.498308 3:+82623299 None:intergenic
TGAACTCTTAGTAGAAATCA+TGG 0.498516 3:-82621172 MsG0380016373.01.T01:CDS
TTCAATTAGAGTGAGATCTA+CGG 0.500778 3:-82621418 MsG0380016373.01.T01:intron
GCGATACACGCTGGAGACTC+AGG 0.502315 3:-82621381 MsG0380016373.01.T01:CDS
CTGTTGATGTTCATGTTTGG+CGG 0.502808 3:-82622466 MsG0380016373.01.T01:CDS
TGACTTAGTCGTTGCGAACA+TGG 0.512375 3:-82621115 MsG0380016373.01.T01:CDS
GGTGTTGAAGGTGGAAGAGG+AGG 0.515246 3:+82623343 None:intergenic
CTTTCCAAGTTACCTGGAGT+TGG 0.517490 3:-82622545 MsG0380016373.01.T01:CDS
GAGGTAAGGATAGTTCTTGT+CGG 0.518734 3:+82623245 None:intergenic
GAAGATGTTTGAGGGAAATG+AGG 0.519541 3:+82623146 None:intergenic
TTTCCAGCGGAAGGTTTGAC+CGG 0.521741 3:+82623210 None:intergenic
CCCTCCTACCTTTACACTTG+AGG 0.524416 3:-82621586 MsG0380016373.01.T01:CDS
TCCCTCAAACATCTTCAAAA+CGG 0.526912 3:-82623139 MsG0380016373.01.T01:CDS
AAACTGAATCAAGCATTCAA+AGG 0.527556 3:+82622934 None:intergenic
CCACCAACCTCTCAAAATCC+CGG 0.529694 3:-82623291 MsG0380016373.01.T01:CDS
TGTTGAGAGGGATCCATACC+CGG 0.530592 3:+82623272 None:intergenic
CCCTCAAGTGTAAAGGTAGG+AGG 0.533406 3:+82621585 None:intergenic
AGAGGTGGACAGTGAACTAC+TGG 0.535788 3:-82621142 MsG0380016373.01.T01:CDS
GGAGGGAGATTGGGAGTGAA+GGG 0.536641 3:+82623102 None:intergenic
GTGTTTCTCTTGCTGACACG+TGG 0.537150 3:-82623036 MsG0380016373.01.T01:CDS
TCTTGTCGGGTTATGTTGAG+AGG 0.537793 3:+82623259 None:intergenic
CCAGCGGAAGGTTTGACCGG+TGG 0.538070 3:+82623213 None:intergenic
GCAACATTTGAGTGGTGCTA+GGG 0.540107 3:-82622972 MsG0380016373.01.T01:CDS
ATGTTTGAGGGAAATGAGGT+GGG 0.544232 3:+82623150 None:intergenic
CCACCGGTCAAACCTTCCGC+TGG 0.544466 3:-82623213 MsG0380016373.01.T01:CDS
GAAAACAGCGAAGAAGAAGC+TGG 0.546776 3:-82621530 MsG0380016373.01.T01:intron
TCTGAACTTTCCAAGTTACC+TGG 0.547907 3:-82622551 MsG0380016373.01.T01:CDS
GGATTCCGATGAAAGATTTG+CGG 0.549689 3:-82623002 MsG0380016373.01.T01:CDS
ATATTAGTAGAATCACGAAA+TGG 0.554146 3:-82622892 MsG0380016373.01.T01:intron
TGCAATCAAAGCCTGAAGGT+GGG 0.559250 3:-82622622 MsG0380016373.01.T01:CDS
GCAATCAAAGCCTGAAGGTG+GGG 0.559780 3:-82622621 MsG0380016373.01.T01:CDS
CAGTTCCGCAAATCTTTCAT+CGG 0.560607 3:+82622997 None:intergenic
CTCCAACGATGATTGCAAAA+CGG 0.563244 3:-82623083 MsG0380016373.01.T01:CDS
TGTTGATGTTCATGTTTGGC+GGG 0.563445 3:-82622465 MsG0380016373.01.T01:intron
GTTGAGAGGGATCCATACCC+GGG 0.563985 3:+82623273 None:intergenic
TTTGTTGGCAGAGTAAATAG+TGG 0.564187 3:-82621083 None:intergenic
ACCTGGAGTTGGTCCTAAAG+TGG 0.565307 3:-82622534 MsG0380016373.01.T01:CDS
CCCTAACCTTCAATTGACAC+AGG 0.567083 3:-82621202 MsG0380016373.01.T01:CDS
GGTGGGGAAGGTGAGAGGTA+AGG 0.569427 3:+82623231 None:intergenic
GAGTCTAAGGCGATACACGC+TGG 0.570449 3:-82621390 MsG0380016373.01.T01:CDS
TTGCAATCAAAGCCTGAAGG+TGG 0.570829 3:-82622623 MsG0380016373.01.T01:CDS
CACCAACCTCTCAAAATCCC+GGG 0.574837 3:-82623290 MsG0380016373.01.T01:CDS
TGACCGGTGGGGAAGGTGAG+AGG 0.575831 3:+82623226 None:intergenic
TTACCTCTCACCTTCCCCAC+CGG 0.576560 3:-82623229 MsG0380016373.01.T01:CDS
CTTGTCGGGTTATGTTGAGA+GGG 0.578835 3:+82623260 None:intergenic
CAAGATATTGAGATAAAACA+TGG 0.581652 3:-82621342 MsG0380016373.01.T01:intron
TTCTCTTGCTGACACGTGGA+AGG 0.591357 3:-82623032 MsG0380016373.01.T01:CDS
TTGGTGTCCCTCAAGTGTAA+AGG 0.593031 3:+82621578 None:intergenic
AACATGAACATCAACAGGAA+TGG 0.597125 3:+82622472 None:intergenic
AGCGGAAGGTTTGACCGGTG+GGG 0.603850 3:+82623215 None:intergenic
GGAGGAGGTAGTGATTTGGT+GGG 0.604507 3:+82623361 None:intergenic
GCCACTTTAGGACCAACTCC+AGG 0.606013 3:+82622533 None:intergenic
ACGACGCCGGTGAATTGAGA+AGG 0.607725 3:+82623178 None:intergenic
AGAAATCATGGTCGAAGCAG+AGG 0.608867 3:-82621160 MsG0380016373.01.T01:CDS
GCCGGTGAATTGAGAAGGAG+CGG 0.615116 3:+82623183 None:intergenic
CCTCAAGTGTAAAGGTAGGA+GGG 0.619016 3:+82621586 None:intergenic
AATCATGGTCGAAGCAGAGG+TGG 0.620518 3:-82621157 MsG0380016373.01.T01:CDS
ACCGCTCCTTCTCAATTCAC+CGG 0.631136 3:-82623184 MsG0380016373.01.T01:CDS
GAGGTGGGAGCCAACGACGC+CGG 0.631246 3:+82623165 None:intergenic
TAACCTTCAATTGACACAGG+TGG 0.641383 3:-82621199 MsG0380016373.01.T01:CDS
GATGTTTGAGGGAAATGAGG+TGG 0.643569 3:+82623149 None:intergenic
CGTGTCAGCAAGAGAAACAC+CGG 0.651572 3:+82623039 None:intergenic
GAACATCAACAGGAATGGCA+TGG 0.655759 3:+82622477 None:intergenic
GCTTTGCAATCAAAGCCTGA+AGG 0.659391 3:-82622626 MsG0380016373.01.T01:CDS
TGTCCCTCAAGTGTAAAGGT+AGG 0.665958 3:+82621582 None:intergenic
CCTGTGTCAATTGAAGGTTA+GGG 0.683122 3:+82621202 None:intergenic
GAACTGGCGCAACATTTGAG+TGG 0.700408 3:-82622980 MsG0380016373.01.T01:CDS
GGTGGACAGTGAACTACTGG+CGG 0.704341 3:-82621139 MsG0380016373.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAATAATCTAATTACAG+AGG + Chr3:82622583-82622602 None:intergenic 15.0%
!! TTCTAATGTTGTAATAAAAT+TGG - Chr3:82622082-82622101 MsG0380016373.01.T01:intron 15.0%
!!! TTGTTGTATTATTTTTTCAT+GGG - Chr3:82622519-82622538 MsG0380016373.01.T01:CDS 15.0%
!! AAACTATAAGAAAAACTGTT+AGG - Chr3:82622388-82622407 MsG0380016373.01.T01:intron 20.0%
!!! GTTGTTGTATTATTTTTTCA+TGG - Chr3:82622518-82622537 MsG0380016373.01.T01:CDS 20.0%
!!! TGTTGTATTATTTTTTCATG+GGG - Chr3:82622520-82622539 MsG0380016373.01.T01:CDS 20.0%
! ATATTAGTAGAATCACGAAA+TGG - Chr3:82621576-82621595 MsG0380016373.01.T01:CDS 25.0%
! CAAGATATTGAGATAAAACA+TGG - Chr3:82623126-82623145 MsG0380016373.01.T01:CDS 25.0%
! GTTCTATCATAAACCTTATT+TGG + Chr3:82622441-82622460 None:intergenic 25.0%
! GTTTCATTACAGATTTAGAA+GGG - Chr3:82622550-82622569 MsG0380016373.01.T01:CDS 25.0%
! TAAACTTGATGTTATAACGT+AGG - Chr3:82622983-82623002 MsG0380016373.01.T01:CDS 25.0%
! TTGTTACCTATCAATAAGAT+GGG - Chr3:82622055-82622074 MsG0380016373.01.T01:intron 25.0%
! TTTGTTACCTATCAATAAGA+TGG - Chr3:82622054-82622073 MsG0380016373.01.T01:intron 25.0%
!! AAGACAAATTTGATAGCAAA+AGG - Chr3:82622149-82622168 MsG0380016373.01.T01:intron 25.0%
!! AGACAAATTTGATAGCAAAA+GGG - Chr3:82622150-82622169 MsG0380016373.01.T01:intron 25.0%
!! CTTTTATCATTTTCAAACTG+GGG - Chr3:82622702-82622721 MsG0380016373.01.T01:intron 25.0%
!! GCTTTTATCATTTTCAAACT+GGG - Chr3:82622701-82622720 MsG0380016373.01.T01:intron 25.0%
!! GTTTTTCTTATAGTTTTTCC+AGG + Chr3:82622384-82622403 None:intergenic 25.0%
!! TGAAGTATTTAAGCAAAAGT+AGG + Chr3:82622032-82622051 None:intergenic 25.0%
!! TGCTTTTATCATTTTCAAAC+TGG - Chr3:82622700-82622719 MsG0380016373.01.T01:intron 25.0%
!!! ATCTTGTAGAGTTTTATGAT+TGG - Chr3:82622652-82622671 MsG0380016373.01.T01:CDS 25.0%
!!! ATTATTTTTTTCCGTGGTTT+AGG - Chr3:82622188-82622207 MsG0380016373.01.T01:intron 25.0%
!!! GTTGTATTATTTTTTCATGG+GGG - Chr3:82622521-82622540 MsG0380016373.01.T01:CDS 25.0%
AAACTGAATCAAGCATTCAA+AGG + Chr3:82621537-82621556 None:intergenic 30.0%
AAATTGCCCATCTTATTGAT+AGG + Chr3:82622064-82622083 None:intergenic 30.0%
AACAAAGTATGGTAATATGC+CGG - Chr3:82622812-82622831 MsG0380016373.01.T01:intron 30.0%
AACTGAATCAAGCATTCAAA+GGG + Chr3:82621536-82621555 None:intergenic 30.0%
AACTGTTAGGAAATATGTAG+AGG - Chr3:82622401-82622420 MsG0380016373.01.T01:intron 30.0%
ACTTATTTAGGTGAAGTTGA+AGG - Chr3:82621622-82621641 MsG0380016373.01.T01:intron 30.0%
ATATGTAGAGGTTAATGAGT+TGG - Chr3:82622413-82622432 MsG0380016373.01.T01:intron 30.0%
CTTATTTAGGTGAAGTTGAA+GGG - Chr3:82621623-82621642 MsG0380016373.01.T01:intron 30.0%
GATAGGGCTAAATATATAAC+TGG - Chr3:82621809-82621828 MsG0380016373.01.T01:intron 30.0%
GGTTTCATTACAGATTTAGA+AGG - Chr3:82622549-82622568 MsG0380016373.01.T01:CDS 30.0%
TGAACTCTTAGTAGAAATCA+TGG - Chr3:82623296-82623315 MsG0380016373.01.T01:CDS 30.0%
TTCAATTAGAGTGAGATCTA+CGG - Chr3:82623050-82623069 MsG0380016373.01.T01:CDS 30.0%
! TAATGAGTTGGATCCAAATA+AGG - Chr3:82622425-82622444 MsG0380016373.01.T01:intron 30.0%
!! TTATACCACTTTTCTACTGT+AGG + Chr3:82622277-82622296 None:intergenic 30.0%
!!! GTTGTCATTATTTTTTTCCG+TGG - Chr3:82622182-82622201 MsG0380016373.01.T01:intron 30.0%
AACATGAACATCAACAGGAA+TGG + Chr3:82621999-82622018 None:intergenic 35.0%
ACATGGAAGATGAACTTTGT+TGG - Chr3:82623370-82623389 MsG0380016373.01.T01:CDS 35.0%
ACGAAGAGTAGATTAGATGT+AGG - Chr3:82622331-82622350 MsG0380016373.01.T01:intron 35.0%
GTATAAAACAGTTGAGCTGT+AGG - Chr3:82622291-82622310 MsG0380016373.01.T01:intron 35.0%
GTTCAGAAACTAATGAAAGC+TGG + Chr3:82621677-82621696 None:intergenic 35.0%
TCAATTAGAGTGAGATCTAC+GGG - Chr3:82623051-82623070 MsG0380016373.01.T01:CDS 35.0%
TCCCTCAAACATCTTCAAAA+CGG - Chr3:82621329-82621348 MsG0380016373.01.T01:intron 35.0%
TGCTATCATTTATGCATGAC+TGG - Chr3:82622622-82622641 MsG0380016373.01.T01:CDS 35.0%
TTCCTGTTGATGTTCATGTT+TGG - Chr3:82621999-82622018 MsG0380016373.01.T01:intron 35.0%
TTGATTACGTTTCGTCTCTT+GGG - Chr3:82621599-82621618 MsG0380016373.01.T01:CDS 35.0%
TTGCTTGATTTCTTAAACGC+CGG - Chr3:82621410-82621429 MsG0380016373.01.T01:CDS 35.0%
! CTCGGGTAAGATATACTTTT+GGG + Chr3:82622736-82622755 None:intergenic 35.0%
! CTGTTTTCTTCTATCTTTGC+GGG + Chr3:82622926-82622945 None:intergenic 35.0%
! GAGGCTTTTGTAACAAAGTA+TGG - Chr3:82622801-82622820 MsG0380016373.01.T01:intron 35.0%
!! GCTGTTTTCTTCTATCTTTG+CGG + Chr3:82622927-82622946 None:intergenic 35.0%
!! GTGGTTGCTATTTCTTGATA+GGG - Chr3:82621793-82621812 MsG0380016373.01.T01:intron 35.0%
!! TTTTGGTTACAGGTTAGGTT+AGG - Chr3:82621716-82621735 MsG0380016373.01.T01:intron 35.0%
!!! GTATTATTTTTTCATGGGGG+TGG - Chr3:82622524-82622543 MsG0380016373.01.T01:CDS 35.0%
!!! TTTTTTCCGTGGTTTAGGAA+TGG - Chr3:82622193-82622212 MsG0380016373.01.T01:intron 35.0%
ACACCATTAGAGAGAGAGTT+GGG - Chr3:82622245-82622264 MsG0380016373.01.T01:intron 40.0%
ACCTGTGTCAATTGAAGGTT+AGG + Chr3:82623270-82623289 None:intergenic 40.0%
AGGTAAGGATAGTTCTTGTC+GGG + Chr3:82621225-82621244 None:intergenic 40.0%
AGTCAAATCAGAAGAGGTAG+TGG - Chr3:82622354-82622373 MsG0380016373.01.T01:intron 40.0%
ATGTTTGAGGGAAATGAGGT+GGG + Chr3:82621321-82621340 None:intergenic 40.0%
ATTCCTAATCATATCCCGTC+TGG + Chr3:82622224-82622243 None:intergenic 40.0%
CAGTTCCGCAAATCTTTCAT+CGG + Chr3:82621474-82621493 None:intergenic 40.0%
CCTGTGTCAATTGAAGGTTA+GGG + Chr3:82623269-82623288 None:intergenic 40.0%
CTCCAACGATGATTGCAAAA+CGG - Chr3:82621385-82621404 MsG0380016373.01.T01:CDS 40.0%
CTGTTGATGTTCATGTTTGG+CGG - Chr3:82622002-82622021 MsG0380016373.01.T01:intron 40.0%
GAAGATGTTTGAGGGAAATG+AGG + Chr3:82621325-82621344 None:intergenic 40.0%
GAGGTAAGGATAGTTCTTGT+CGG + Chr3:82621226-82621245 None:intergenic 40.0%
GGATTCCGATGAAAGATTTG+CGG - Chr3:82621466-82621485 MsG0380016373.01.T01:intron 40.0%
GTCTTACCATTCCTAAACCA+CGG + Chr3:82622202-82622221 None:intergenic 40.0%
GTGAAGTTTCTGTGTCAACT+TGG + Chr3:82622769-82622788 None:intergenic 40.0%
GTTGATTACGTTTCGTCTCT+TGG - Chr3:82621598-82621617 MsG0380016373.01.T01:CDS 40.0%
GTTTCGTGTGAATGTTAACG+TGG - Chr3:82621774-82621793 MsG0380016373.01.T01:intron 40.0%
TAACCTTCAATTGACACAGG+TGG - Chr3:82623269-82623288 MsG0380016373.01.T01:CDS 40.0%
TAGCTCCTACAGTAGAAAAG+TGG - Chr3:82622269-82622288 MsG0380016373.01.T01:intron 40.0%
TATCCACCTGTGTCAATTGA+AGG + Chr3:82623275-82623294 None:intergenic 40.0%
TCGTCTCTTGGGACTTATTT+AGG - Chr3:82621610-82621629 MsG0380016373.01.T01:intron 40.0%
TCTGAACTTTCCAAGTTACC+TGG - Chr3:82621917-82621936 MsG0380016373.01.T01:intron 40.0%
TGTAGGAGTCAAATCAGAAG+AGG - Chr3:82622348-82622367 MsG0380016373.01.T01:intron 40.0%
TGTTGATGTTCATGTTTGGC+GGG - Chr3:82622003-82622022 MsG0380016373.01.T01:intron 40.0%
TTAGGAATGGTAAGACCAGA+CGG - Chr3:82622206-82622225 MsG0380016373.01.T01:intron 40.0%
! GCTCGGGTAAGATATACTTT+TGG + Chr3:82622737-82622756 None:intergenic 40.0%
! GCTTTATTCTCTTCGCACAT+TGG - Chr3:82621877-82621896 MsG0380016373.01.T01:intron 40.0%
! GTTTTGCAATCATCGTTGGA+GGG + Chr3:82621386-82621405 None:intergenic 40.0%
!! CGTGGTTGCTATTTCTTGAT+AGG - Chr3:82621792-82621811 MsG0380016373.01.T01:intron 40.0%
!!! GCCGTTTTGAAGATGTTTGA+GGG + Chr3:82621333-82621352 None:intergenic 40.0%
!!! TATTTTTTCATGGGGGTGGA+GGG - Chr3:82622528-82622547 MsG0380016373.01.T01:CDS 40.0%
!!! TTATTTTTTCATGGGGGTGG+AGG - Chr3:82622527-82622546 MsG0380016373.01.T01:CDS 40.0%
AACGTAGGCGTTCGAATATG+CGG - Chr3:82622998-82623017 MsG0380016373.01.T01:CDS 45.0%
AAGAGGAGGAGGTAGTGATT+TGG + Chr3:82621114-82621133 None:intergenic 45.0%
AAGTATGGTAATATGCCGGC+TGG - Chr3:82622816-82622835 MsG0380016373.01.T01:intron 45.0%
AATCATCGTTGGAGGGAGAT+TGG + Chr3:82621379-82621398 None:intergenic 45.0%
ACTGCTGAGTGATGAATGAG+GGG - Chr3:82623156-82623175 MsG0380016373.01.T01:CDS 45.0%
AGAAATCATGGTCGAAGCAG+AGG - Chr3:82623308-82623327 MsG0380016373.01.T01:CDS 45.0%
AGACCAGACGGGATATGATT+AGG - Chr3:82622218-82622237 MsG0380016373.01.T01:intron 45.0%
ATCATCGTTGGAGGGAGATT+GGG + Chr3:82621378-82621397 None:intergenic 45.0%
CACCATTAGAGAGAGAGTTG+GGG - Chr3:82622246-82622265 MsG0380016373.01.T01:intron 45.0%
CACTGCTGAGTGATGAATGA+GGG - Chr3:82623155-82623174 MsG0380016373.01.T01:CDS 45.0%
CCCTAACCTTCAATTGACAC+AGG - Chr3:82623266-82623285 MsG0380016373.01.T01:CDS 45.0%
CCTCAAGTGTAAAGGTAGGA+GGG + Chr3:82622885-82622904 None:intergenic 45.0%
CCTCCTACCTTTACACTTGA+GGG - Chr3:82622883-82622902 MsG0380016373.01.T01:intron 45.0%
CGATGAAAGATTTGCGGAAC+TGG - Chr3:82621472-82621491 MsG0380016373.01.T01:intron 45.0%
CGCCAAACATGAACATCAAC+AGG + Chr3:82622004-82622023 None:intergenic 45.0%
CTTGTCGGGTTATGTTGAGA+GGG + Chr3:82621211-82621230 None:intergenic 45.0%
CTTTCCAAGTTACCTGGAGT+TGG - Chr3:82621923-82621942 MsG0380016373.01.T01:intron 45.0%
GAAAACAGCGAAGAAGAAGC+TGG - Chr3:82622938-82622957 MsG0380016373.01.T01:CDS 45.0%
GAACATCAACAGGAATGGCA+TGG + Chr3:82621994-82622013 None:intergenic 45.0%
GACACCATTAGAGAGAGAGT+TGG - Chr3:82622244-82622263 MsG0380016373.01.T01:intron 45.0%
GATGTTTGAGGGAAATGAGG+TGG + Chr3:82621322-82621341 None:intergenic 45.0%
GCTTTGCAATCAAAGCCTGA+AGG - Chr3:82621842-82621861 MsG0380016373.01.T01:intron 45.0%
TACCCCAACTCTCTCTCTAA+TGG + Chr3:82622251-82622270 None:intergenic 45.0%
TAGGAATGGTAAGACCAGAC+GGG - Chr3:82622207-82622226 MsG0380016373.01.T01:intron 45.0%
TATATCTTACCCGAGCTTGC+AGG - Chr3:82622741-82622760 MsG0380016373.01.T01:intron 45.0%
TCACTGCTGAGTGATGAATG+AGG - Chr3:82623154-82623173 MsG0380016373.01.T01:CDS 45.0%
TCTTGTCGGGTTATGTTGAG+AGG + Chr3:82621212-82621231 None:intergenic 45.0%
TGACTTAGTCGTTGCGAACA+TGG - Chr3:82623353-82623372 MsG0380016373.01.T01:CDS 45.0%
TGCAATCAAAGCCTGAAGGT+GGG - Chr3:82621846-82621865 MsG0380016373.01.T01:intron 45.0%
TGCTGAGTTGCCTTCACAAA+AGG - Chr3:82622859-82622878 MsG0380016373.01.T01:intron 45.0%
TGTCCCTCAAGTGTAAAGGT+AGG + Chr3:82622889-82622908 None:intergenic 45.0%
TTGCAATCAAAGCCTGAAGG+TGG - Chr3:82621845-82621864 MsG0380016373.01.T01:intron 45.0%
! AAGCCTTATCCCACTAAGTG+GGG + Chr3:82622494-82622513 None:intergenic 45.0%
! AGTATGGTAATATGCCGGCT+GGG - Chr3:82622817-82622836 MsG0380016373.01.T01:intron 45.0%
! ATCTACGGGTTGTGAGTCTA+AGG - Chr3:82623065-82623084 MsG0380016373.01.T01:CDS 45.0%
! CAAGCCTTATCCCACTAAGT+GGG + Chr3:82622495-82622514 None:intergenic 45.0%
! CGTTTTGCAATCATCGTTGG+AGG + Chr3:82621387-82621406 None:intergenic 45.0%
! GCAACATTTGAGTGGTGCTA+GGG - Chr3:82621496-82621515 MsG0380016373.01.T01:intron 45.0%
! GCTATACAAGCAGCCACTTT+AGG + Chr3:82621950-82621969 None:intergenic 45.0%
! TGAGTGATGAATGAGGGTGT+AGG - Chr3:82623161-82623180 MsG0380016373.01.T01:CDS 45.0%
! TTGGTGTCCCTCAAGTGTAA+AGG + Chr3:82622893-82622912 None:intergenic 45.0%
!!! ATTTTGAGAGGTTGGTGGAG+TGG + Chr3:82621175-82621194 None:intergenic 45.0%
!!! CAACTAAGAGACGCTGGTTT+TGG - Chr3:82621699-82621718 MsG0380016373.01.T01:intron 45.0%
!!! CGCCGTTTTGAAGATGTTTG+AGG + Chr3:82621334-82621353 None:intergenic 45.0%
!!! GCTGGTTTTGGTTACAGGTT+AGG - Chr3:82621711-82621730 MsG0380016373.01.T01:intron 45.0%
!!! TGCCGTTTTGCAATCATCGT+TGG + Chr3:82621390-82621409 None:intergenic 45.0%
AACTTGGAACCTGCAAGCTC+GGG + Chr3:82622753-82622772 None:intergenic 50.0%
AATCATGGTCGAAGCAGAGG+TGG - Chr3:82623311-82623330 MsG0380016373.01.T01:CDS 50.0%
ACCGCTCCTTCTCAATTCAC+CGG - Chr3:82621284-82621303 MsG0380016373.01.T01:intron 50.0%
ACCTCTCAAAATCCCGGGTA+TGG - Chr3:82621183-82621202 MsG0380016373.01.T01:CDS 50.0%
ACTTCACAGTCGTGTTGCAG+AGG - Chr3:82622782-82622801 MsG0380016373.01.T01:intron 50.0%
AGAGGTGGACAGTGAACTAC+TGG - Chr3:82623326-82623345 MsG0380016373.01.T01:CDS 50.0%
AGGACCAACTCCAGGTAACT+TGG + Chr3:82621930-82621949 None:intergenic 50.0%
CAACTTGGAACCTGCAAGCT+CGG + Chr3:82622754-82622773 None:intergenic 50.0%
CACCAACCTCTCAAAATCCC+GGG - Chr3:82621178-82621197 MsG0380016373.01.T01:CDS 50.0%
CCACCAACCTCTCAAAATCC+CGG - Chr3:82621177-82621196 MsG0380016373.01.T01:CDS 50.0%
CCACTTAGTGGGATAAGGCT+TGG - Chr3:82622493-82622512 MsG0380016373.01.T01:CDS 50.0%
CCCTCAAGTGTAAAGGTAGG+AGG + Chr3:82622886-82622905 None:intergenic 50.0%
CCCTCCTACCTTTACACTTG+AGG - Chr3:82622882-82622901 MsG0380016373.01.T01:intron 50.0%
CGCAACATTTGAGTGGTGCT+AGG - Chr3:82621495-82621514 MsG0380016373.01.T01:intron 50.0%
CGTGTCAGCAAGAGAAACAC+CGG + Chr3:82621432-82621451 None:intergenic 50.0%
CTTATCCCACTAAGTGGGGT+CGG + Chr3:82622490-82622509 None:intergenic 50.0%
CTTTGCGGGATTCTTCTGCT+TGG + Chr3:82622912-82622931 None:intergenic 50.0%
GAACAGCAACTAAGAGACGC+TGG - Chr3:82621693-82621712 MsG0380016373.01.T01:intron 50.0%
GAACTGGCGCAACATTTGAG+TGG - Chr3:82621488-82621507 MsG0380016373.01.T01:intron 50.0%
GCAATCAAAGCCTGAAGGTG+GGG - Chr3:82621847-82621866 MsG0380016373.01.T01:intron 50.0%
GTGTTTCTCTTGCTGACACG+TGG - Chr3:82621432-82621451 MsG0380016373.01.T01:intron 50.0%
TAAAACAGCTTGAGCCCAGC+CGG + Chr3:82622834-82622853 None:intergenic 50.0%
TGTTGAGAGGGATCCATACC+CGG + Chr3:82621199-82621218 None:intergenic 50.0%
TTCTCTTGCTGACACGTGGA+AGG - Chr3:82621436-82621455 MsG0380016373.01.T01:intron 50.0%
TTTCCAGCGGAAGGTTTGAC+CGG + Chr3:82621261-82621280 None:intergenic 50.0%
! AAGGAGCGGTGTTTTTCCAG+CGG + Chr3:82621274-82621293 None:intergenic 50.0%
! ACCTGGAGTTGGTCCTAAAG+TGG - Chr3:82621934-82621953 MsG0380016373.01.T01:intron 50.0%
! AGGAGGAGGTAGTGATTTGG+TGG + Chr3:82621111-82621130 None:intergenic 50.0%
! CCAAGCCTTATCCCACTAAG+TGG + Chr3:82622496-82622515 None:intergenic 50.0%
! TCCATACCCGGGATTTTGAG+AGG + Chr3:82621187-82621206 None:intergenic 50.0%
!! AGAAGAGGTAGTGGAAGACC+TGG - Chr3:82622363-82622382 MsG0380016373.01.T01:intron 50.0%
!! GGAGGAGGTAGTGATTTGGT+GGG + Chr3:82621110-82621129 None:intergenic 50.0%
!! TACCCGGGATTTTGAGAGGT+TGG + Chr3:82621183-82621202 None:intergenic 50.0%
!! TGACACGTGGAAGGTGTTCT+CGG - Chr3:82621445-82621464 MsG0380016373.01.T01:intron 50.0%
!!! GAGACGCTGGTTTTGGTTAC+AGG - Chr3:82621706-82621725 MsG0380016373.01.T01:intron 50.0%
ACGACGCCGGTGAATTGAGA+AGG + Chr3:82621293-82621312 None:intergenic 55.0%
ACTAAGTGGGGTCGGCTACA+TGG + Chr3:82622482-82622501 None:intergenic 55.0%
AGCCATTCTTCCCCACCTTC+AGG + Chr3:82621860-82621879 None:intergenic 55.0%
AGCCTGAAGGTGGGGAAGAA+TGG - Chr3:82621855-82621874 MsG0380016373.01.T01:intron 55.0%
ATGTAGCCGACCCCACTTAG+TGG - Chr3:82622481-82622500 MsG0380016373.01.T01:CDS 55.0%
CGACCCCACTTAGTGGGATA+AGG - Chr3:82622488-82622507 MsG0380016373.01.T01:CDS 55.0%
GCCGGTGAATTGAGAAGGAG+CGG + Chr3:82621288-82621307 None:intergenic 55.0%
GGAGGGAGATTGGGAGTGAA+GGG + Chr3:82621369-82621388 None:intergenic 55.0%
GGTGGACAGTGAACTACTGG+CGG - Chr3:82623329-82623348 MsG0380016373.01.T01:CDS 55.0%
GTTGAAGGTGGAAGAGGAGG+AGG + Chr3:82621125-82621144 None:intergenic 55.0%
GTTGAGAGGGATCCATACCC+GGG + Chr3:82621198-82621217 None:intergenic 55.0%
TCTCAATTCACCGGCGTCGT+TGG - Chr3:82621293-82621312 MsG0380016373.01.T01:intron 55.0%
TGGAGGGAGATTGGGAGTGA+AGG + Chr3:82621370-82621389 None:intergenic 55.0%
TGTAGCCGACCCCACTTAGT+GGG - Chr3:82622482-82622501 MsG0380016373.01.T01:CDS 55.0%
TTACCTCTCACCTTCCCCAC+CGG - Chr3:82621239-82621258 MsG0380016373.01.T01:CDS 55.0%
! AGCGGTGTTTTTCCAGCGGA+AGG + Chr3:82621270-82621289 None:intergenic 55.0%
! GAGTCTAAGGCGATACACGC+TGG - Chr3:82623078-82623097 MsG0380016373.01.T01:CDS 55.0%
! GAGTGATGAATGAGGGGTGC+AGG - Chr3:82623210-82623229 MsG0380016373.01.T01:CDS 55.0%
! GGTAGGAGGGCCTTTTGTGA+AGG + Chr3:82622872-82622891 None:intergenic 55.0%
! GGTGGTGTTGAAGGTGGAAG+AGG + Chr3:82621131-82621150 None:intergenic 55.0%
! GGTGTTGAAGGTGGAAGAGG+AGG + Chr3:82621128-82621147 None:intergenic 55.0%
!! CCGGGATTTTGAGAGGTTGG+TGG + Chr3:82621180-82621199 None:intergenic 55.0%
!! GCCACTTTAGGACCAACTCC+AGG + Chr3:82621938-82621957 None:intergenic 55.0%
!! GGTGTTGGTGGTGTTGAAGG+TGG + Chr3:82621137-82621156 None:intergenic 55.0%
!! GTTTGGGTTTGGGGTGTTGG+TGG + Chr3:82621149-82621168 None:intergenic 55.0%
!! TGGGGTGTTGGTGGTGTTGA+AGG + Chr3:82621140-82621159 None:intergenic 55.0%
!! TTGAGAGGTTGGTGGAGTGG+CGG + Chr3:82621172-82621191 None:intergenic 55.0%
AGCGGAAGGTTTGACCGGTG+GGG + Chr3:82621256-82621275 None:intergenic 60.0%
CAGCGGAAGGTTTGACCGGT+GGG + Chr3:82621257-82621276 None:intergenic 60.0%
GAAGGTTTGACCGGTGGGGA+AGG + Chr3:82621252-82621271 None:intergenic 60.0%
GCGATACACGCTGGAGACTC+AGG - Chr3:82623087-82623106 MsG0380016373.01.T01:CDS 60.0%
GGTGGGGAAGGTGAGAGGTA+AGG + Chr3:82621240-82621259 None:intergenic 60.0%
! CGGGTTTGGGTTTGGGGTGT+TGG + Chr3:82621152-82621171 None:intergenic 60.0%
!! GAGTGGCGGGTTTGGGTTTG+GGG + Chr3:82621158-82621177 None:intergenic 60.0%
!! GGAGTGGCGGGTTTGGGTTT+GGG + Chr3:82621159-82621178 None:intergenic 60.0%
!! GTTGGTGGAGTGGCGGGTTT+GGG + Chr3:82621165-82621184 None:intergenic 60.0%
!! TGAGAGGTTGGTGGAGTGGC+GGG + Chr3:82621171-82621190 None:intergenic 60.0%
!! TGGAGTGGCGGGTTTGGGTT+TGG + Chr3:82621160-82621179 None:intergenic 60.0%
CCACCGGTCAAACCTTCCGC+TGG - Chr3:82621255-82621274 MsG0380016373.01.T01:intron 65.0%
CCAGCGGAAGGTTTGACCGG+TGG + Chr3:82621258-82621277 None:intergenic 65.0%
TGACCGGTGGGGAAGGTGAG+AGG + Chr3:82621245-82621264 None:intergenic 65.0%
!! GGTTGGTGGAGTGGCGGGTT+TGG + Chr3:82621166-82621185 None:intergenic 65.0%
GAGGTGGGAGCCAACGACGC+CGG + Chr3:82621306-82621325 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr3 gene 82621086 82623404 82621086 ID=MsG0380016373.01;Name=MsG0380016373.01
Chr3 mRNA 82621086 82623404 82621086 ID=MsG0380016373.01.T01;Parent=MsG0380016373.01;Name=MsG0380016373.01.T01;_AED=0.32;_eAED=0.48;_QI=0|0|0.2|1|1|1|5|0|358
Chr3 exon 82622893 82623404 82622893 ID=MsG0380016373.01.T01:exon:33142;Parent=MsG0380016373.01.T01
Chr3 exon 82622466 82622676 82622466 ID=MsG0380016373.01.T01:exon:33141;Parent=MsG0380016373.01.T01
Chr3 exon 82621531 82621624 82621531 ID=MsG0380016373.01.T01:exon:33140;Parent=MsG0380016373.01.T01
Chr3 exon 82621343 82621429 82621343 ID=MsG0380016373.01.T01:exon:33139;Parent=MsG0380016373.01.T01
Chr3 exon 82621086 82621258 82621086 ID=MsG0380016373.01.T01:exon:33138;Parent=MsG0380016373.01.T01
Chr3 CDS 82622893 82623404 82622893 ID=MsG0380016373.01.T01:cds;Parent=MsG0380016373.01.T01
Chr3 CDS 82622466 82622676 82622466 ID=MsG0380016373.01.T01:cds;Parent=MsG0380016373.01.T01
Chr3 CDS 82621531 82621624 82621531 ID=MsG0380016373.01.T01:cds;Parent=MsG0380016373.01.T01
Chr3 CDS 82621343 82621429 82621343 ID=MsG0380016373.01.T01:cds;Parent=MsG0380016373.01.T01
Chr3 CDS 82621086 82621258 82621086 ID=MsG0380016373.01.T01:cds;Parent=MsG0380016373.01.T01
Gene Sequence

>MsG0380016373.01.T01

ATGACGAAGAGAAAAAGATCACCCACCAAATCACTACCTCCTCCTCTTCCACCTTCAACACCACCAACACCCCAAACCCAAACCCGCCACTCCACCAACCTCTCAAAATCCCGGGTATGGATCCCTCTCAACATAACCCGACAAGAACTATCCTTACCTCTCACCTTCCCCACCGGTCAAACCTTCCGCTGGAAAAACACCGCTCCTTCTCAATTCACCGGCGTCGTTGGCTCCCACCTCATTTCCCTCAAACATCTTCAAAACGGCGACGTTTGCTACACCCTTCACTCCCAATCTCCCTCCAACGATGATTGCAAAACGGCATTGCTTGATTTCTTAAACGCCGGTGTTTCTCTTGCTGACACGTGGAAGGTGTTCTCGGATTCCGATGAAAGATTTGCGGAACTGGCGCAACATTTGAGTGGTGCTAGGGTTTTGAGACAAGATCCCTTTGAATGCTTGATTCAGTTTATGTGTTCTTCTAATAATCATATTAGTAGAATCACGAAATGGGCTAAATATATAACTGGCACTGTAAATGCTTTGCAATCAAAGCCTGAAGGTGGGGAAGAATGGCTTTATTCTCTTCGCACATTGGAGCTTGAAGATGTAATATCTGAACTTTCCAAGTTACCTGGAGTTGGTCCTAAAGTGGCTGCTTGTATAGCTCTTTACTCACTTGATCAGCACCATGCCATTCCTGTTGATGTTCATGTTTGGCGGTTGCCTTCACAAAAGGCCCTCCTACCTTTACACTTGAGGGACACCAAGCAGAAGAATCCCGCAAAGATAGAAGAAAACAGCGAAGAAGAAGCTGTGAGATCTACGGGTTGTGAGTCTAAGGCGATACACGCTGGAGACTCAGGCGCGACAGACTGCAATCAAGATATTGAGATAAAACATGGTTTTTCTACTATGCATATTGTTAACAACTCTTTCCCTAACCTTCAATTGACACAGGTGGATAATGAACTCTTAGTAGAAATCATGGTCGAAGCAGAGGTGGACAGTGAACTACTGGCGGATGACTTAGTCGTTGCGAACATGGAAGATGAACTTTGTTGGCAGAGTAAATAG
Protein sequence

>MsG0380016373.01.T01

MTKRKRSPTKSLPPPLPPSTPPTPQTQTRHSTNLSKSRVWIPLNITRQELSLPLTFPTGQTFRWKNTAPSQFTGVVGSHLISLKHLQNGDVCYTLHSQSPSNDDCKTALLDFLNAGVSLADTWKVFSDSDERFAELAQHLSGARVLRQDPFECLIQFMCSSNNHISRITKWAKYITGTVNALQSKPEGGEEWLYSLRTLELEDVISELSKLPGVGPKVAACIALYSLDQHHAIPVDVHVWRLPSQKALLPLHLRDTKQKNPAKIEENSEEEAVRSTGCESKAIHAGDSGATDCNQDIEIKHGFSTMHIVNNSFPNLQLTQVDNELLVEIMVEAEVDSELLADDLVVANMEDELCWQSK*