AlfalfaGEDB | MsG0380016639.01

M. sativa cultivar ZhongmuNo.1 / MsG0380016639.01

KEGG: -
GO: GO:0005524 GO:0005634 GO:0006355
PFAM: PF08879 PF08880

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380016639.01.T01	XP_003602338.1	90.26	462	25	7	1	462	1	442	0	768

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380016639.01.T01	Q6AWY6	57.944	107	29	3	127	218	52	157	2.78E-32	130

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380016639.01.T01	G7J891	90.260	462	25	7	1	462	1	442	0.0	768

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TFs/TRs:

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Gene ID	Type	Classification
MsG0380016639.01.T01	TF	GRF

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0380016593.01	MsG0380016639.01	0.825349	4.919784e-54	4.472898e-51

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380016639.01.T01	MTR_3g092330	90.260	462	25	7	1	462	1	442	0.0	768
MsG0380016639.01.T01	MTR_1g017490	52.632	133	37	3	110	218	126	256	1.05e-33	134
MsG0380016639.01.T01	MTR_1g017490	54.331	127	28	4	116	218	3	123	4.13e-33	131
MsG0380016639.01.T01	MTR_0001s0490	53.153	111	35	2	128	221	25	135	2.65e-32	126
MsG0380016639.01.T01	MTR_5g027030	57.000	100	35	2	126	218	14	112	7.54e-30	119
MsG0380016639.01.T01	MTR_8g020560	49.541	109	37	2	129	219	12	120	1.47e-29	118
MsG0380016639.01.T01	MTR_2g041430	50.000	114	35	3	128	219	7	120	4.63e-29	117
MsG0380016639.01.T01	MTR_2g041430	50.000	114	35	3	128	219	7	120	4.77e-29	117
MsG0380016639.01.T01	MTR_2g041430	50.000	114	35	3	128	219	7	120	4.85e-29	117
MsG0380016639.01.T01	MTR_7g104050	44.604	139	50	3	109	225	60	193	8.07e-29	117

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380016639.01.T01	AT5G53660	56.140	114	46	2	126	235	57	170	8.71e-33	128
MsG0380016639.01.T01	AT2G22840	54.310	116	35	2	128	226	133	247	2.83e-31	126
MsG0380016639.01.T01	AT3G13960	51.327	113	37	2	124	218	12	124	4.29e-30	121
MsG0380016639.01.T01	AT4G24150	40.936	171	69	6	102	249	90	251	9.95e-30	120
MsG0380016639.01.T01	AT4G24150	40.936	171	69	6	102	249	128	289	1.85e-29	120
MsG0380016639.01.T01	AT2G36400	52.137	117	35	1	129	224	77	193	1.68e-28	116
MsG0380016639.01.T01	AT3G52910	49.107	112	35	1	129	218	83	194	2.79e-26	110
MsG0380016639.01.T01	AT4G24150	35.714	196	69	6	102	249	128	314	4.28e-26	111
MsG0380016639.01.T01	AT2G06200	42.759	145	48	5	102	216	23	162	1.97e-25	105
MsG0380016639.01.T01	AT2G06200	46.610	118	34	3	128	216	6	123	3.03e-25	104

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Find 142 sgRNAs with CRISPR-Local

Find 197 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGACATGTTGTATGTTG+TGG	0.562174	3:-85978140	None:intergenic
AAACACAAACTAACCTTTAA+GGG	0.361293	3:-85978263	None:intergenic
AAAGACATGTTGTATGTTGT+GGG	0.561732	3:-85978139	None:intergenic
AAATCAAAGGCCGAGAATGG+CGG	0.623807	3:+85977571	MsG0380016639.01.T01:five_prime_UTR
AAATGAAAGCAAACACACTT+TGG	0.498639	3:-85977901	None:intergenic
AACAAACTTGGAATGACCAA+TGG	0.577256	3:+85979654	MsG0380016639.01.T01:CDS
AACAATACTGATCCAGAGGC+TGG	0.566845	3:+85978817	MsG0380016639.01.T01:CDS
AACAATGGTTCTGCATCAGT+TGG	0.557664	3:+85979903	MsG0380016639.01.T01:CDS
AACCTCTTGGTTTCTCAGAT+TGG	0.311180	3:-85979809	None:intergenic
AACTGATGCAGAACCATTGT+TGG	0.462885	3:-85979901	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAGACATGTTGTATGTTG+TGG	-	Chr3:85978143-85978162	None:intergenic	30.0%
AAAGACATGTTGTATGTTGT+GGG	-	Chr3:85978142-85978161	None:intergenic	30.0%
AAAGCAACACATGATGAAAA+AGG	-	Chr3:85978716-85978735	None:intergenic	30.0%
AAATGAAAGCAAACACACTT+TGG	-	Chr3:85977904-85977923	None:intergenic	30.0%
AATAACACTAGTAGTGTTGT+TGG	+	Chr3:85977980-85977999	MsG0380016639.01.T01:CDS	30.0%
ACAAAGACATAGTACATGTT+TGG	-	Chr3:85979370-85979389	None:intergenic	30.0%
AGTTCAGAACTGTTATATAG+TGG	-	Chr3:85979726-85979745	None:intergenic	30.0%
ATACAGATATAAACTCACCT+TGG	-	Chr3:85979192-85979211	None:intergenic	30.0%
ATACATGAAAGAAGATGAGA+AGG	-	Chr3:85978529-85978548	None:intergenic	30.0%
ATTAAGGAAGAAGAGAGAAA+AGG	-	Chr3:85978560-85978579	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	85977548	85980455	85977548	ID=MsG0380016639.01;Name=MsG0380016639.01
Chr3	mRNA	85977548	85980455	85977548	ID=MsG0380016639.01.T01;Parent=MsG0380016639.01;Name=MsG0380016639.01.T01;_AED=0.24;_eAED=0.24;_QI=381\|0.66\|0.75\|1\|1\|1\|4\|382\|462
Chr3	exon	85977548	85978271	85977548	ID=MsG0380016639.01.T01:exon:21819;Parent=MsG0380016639.01.T01
Chr3	exon	85978410	85978544	85978410	ID=MsG0380016639.01.T01:exon:21820;Parent=MsG0380016639.01.T01
Chr3	exon	85978782	85979193	85978782	ID=MsG0380016639.01.T01:exon:21821;Parent=MsG0380016639.01.T01
Chr3	exon	85979575	85980455	85979575	ID=MsG0380016639.01.T01:exon:21822;Parent=MsG0380016639.01.T01
Chr3	five_prime_UTR	85977548	85977928	85977548	ID=MsG0380016639.01.T01:five_prime_utr;Parent=MsG0380016639.01.T01
Chr3	CDS	85977929	85978271	85977929	ID=MsG0380016639.01.T01:cds;Parent=MsG0380016639.01.T01
Chr3	CDS	85978410	85978544	85978410	ID=MsG0380016639.01.T01:cds;Parent=MsG0380016639.01.T01
Chr3	CDS	85978782	85979193	85978782	ID=MsG0380016639.01.T01:cds;Parent=MsG0380016639.01.T01
Chr3	CDS	85979575	85980073	85979575	ID=MsG0380016639.01.T01:cds;Parent=MsG0380016639.01.T01
Chr3	three_prime_UTR	85980074	85980455	85980074	ID=MsG0380016639.01.T01:three_prime_utr;Parent=MsG0380016639.01.T01

Gene Sequence

>MsG0380016639.01.T01

ATGGGTGAGTTTCGCGTTCAAGACAAGGAACCTACAAAAAAAACCAACAACAATAACACTAGTAGTGTTGTTGGGTCGGTGCAGAGTTTTGAAGGTTTCTCTAATAAGAACATGATGATACTGCAACATCATAATCATCACCACCGTTCATTCTTTGATAATCATGATTCTTCTTCTGTTTCTGCTGCTGTTGATGCTGAAGGTTATGGTCCCACAACATACAACATGTCTTTTGGGGGTGATTCTTCTGCTGTAGGTGCTAATGCTATCATTGATAATGGCGGTGTATCCTTAAATTTTCAACCTTTTCACAACATTTCTGCTTCCAACACAACCCTTAAAGCAGGTGGAATGGCGGCTTCAATAGGAATAGGGTATCCTCCTTTCACAATTGCTCAATGGAGAGAGCTTGAAAGACAAGCTATGATTTACAAGTACTTGATGGCTTCACTTCCTGTTCCTTCTCATCTTCTTTCATTGGATGGTGGTTTCAATCTGAGGCTATCAAATAGGAACAATACTGATCCAGAGGCTGGTAGGTGCAGAAGAACAGATGGTAAAAAATGGAGATGTTCAAGAGATGTAGCTCCTAATAACAAGTACTGTGAAAGACATATGCATAGAGGTCGTCCCCGTTCAAGAAAGCCTGTGGAACTTCACACAAATAACAATAACAATAGCAATAGTCATCATCAAATCAAAAGGGCTCGTTGTGATTCTTCTAATTTTTTTTCACTGCCTGATTTTACTGTGGCTGTTCCTCCTAATCCAACTACAAGAAAATATGGATCTTCATCACATTTTCTTGCTTCCACTCCCCTTCATCCTTATCTCCAATCTTCATTTTCTTTGCATAATAGCAGCTTTGAGGCTTCAAATAAGCAACCAAGGGGCTTGGAGTGGATGCTGAATGGAGATCCAATTTCCCTTGGTGCTTCTAATTCGGGATGGAATCCTCTGATGCATAACAACAAACTTGGAATGACCAATGGAAGTTCCTATCATAATAACACTGAATCCCAGTATCTGAATTCATTTCCACTATATAACAGTTCTGAACTAGCTCAACATGAAAAACCCTTTCCTCTCTTACTTAATCCCCTTGTTGTTCCAATGCAAACTCTCCAATCTGAGAAACCAAGAGGTTTTAGTTTCATTGATGCTTGGTCTAATGCAGAGACAGTGGAAAACAATGCCAACACCACCACCACCACCTCCAACAATGGTTCTGCATCAGTTGGTAAATTATCCCTTTCATCACTTGATCTATCAATGGGAGGTAGTGCTGTGAGTGAAGAAATGAGTACTATTGAAATGAATGCTGCGAGTGATGATGATAGTACTAAAATAGCATTCTCAAGTTGGCTCAATCCAGCACAACCTTGGTAG

Protein sequence

>MsG0380016639.01.T01

MGEFRVQDKEPTKKTNNNNTSSVVGSVQSFEGFSNKNMMILQHHNHHHRSFFDNHDSSSVSAAVDAEGYGPTTYNMSFGGDSSAVGANAIIDNGGVSLNFQPFHNISASNTTLKAGGMAASIGIGYPPFTIAQWRELERQAMIYKYLMASLPVPSHLLSLDGGFNLRLSNRNNTDPEAGRCRRTDGKKWRCSRDVAPNNKYCERHMHRGRPRSRKPVELHTNNNNNSNSHHQIKRARCDSSNFFSLPDFTVAVPPNPTTRKYGSSSHFLASTPLHPYLQSSFSLHNSSFEASNKQPRGLEWMLNGDPISLGASNSGWNPLMHNNKLGMTNGSSYHNNTESQYLNSFPLYNSSELAQHEKPFPLLLNPLVVPMQTLQSEKPRGFSFIDAWSNAETVENNANTTTTTSNNGSASVGKLSLSSLDLSMGGSAVSEEMSTIEMNAASDDDSTKIAFSSWLNPAQPW*