AlfalfaGEDB | MsG0380016885.01

M. sativa cultivar ZhongmuNo.1 / MsG0380016885.01

KEGG: K14488
GO: GO:0009733
PFAM: PF02519

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380016885.01.T01	XP_013461602.1	99.057	106	1	0	1	106	1	106	4.00E-73	222

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380016885.01.T01	Q9ZUZ3	38.947	95	52	2	2	96	17	105	5.22E-16	70.9

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380016885.01.T01	A0A072V1B1	99.057	106	1	0	1	106	1	106	1.91e-73	222

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0280010645.01	MsG0380016885.01	0.809156	2.174472e-50	1.267171e-47
MsG0380016885.01	MsG0880045617.01	0.816630	5.008003e-52	3.567263e-49
MsG0380016885.01	MsG0880045709.01	0.814745	1.317248e-51	8.912121e-49

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380016885.01.T01	MTR_3g097120	99.057	106	1	0	1	106	1	106	4.85e-77	222
MsG0380016885.01.T01	MTR_8g094980	69.159	107	26	2	7	106	1	107	2.10e-42	135
MsG0380016885.01.T01	MTR_3g092220	41.379	87	45	1	4	90	28	108	5.25e-17	70.9
MsG0380016885.01.T01	MTR_7g118010	40.244	82	43	1	9	90	23	98	8.96e-17	70.1
MsG0380016885.01.T01	MTR_8g026730	39.024	82	44	1	9	90	27	102	1.37e-16	69.7
MsG0380016885.01.T01	MTR_4g072220	41.463	82	39	2	2	82	19	92	5.61e-14	62.4
MsG0380016885.01.T01	MTR_2g043960	39.759	83	42	1	9	91	76	150	6.96e-14	63.9
MsG0380016885.01.T01	MTR_4g072890	42.857	77	35	2	5	80	22	90	1.14e-13	61.6
MsG0380016885.01.T01	MTR_4g072300	43.590	78	35	2	4	80	21	90	1.14e-13	61.6
MsG0380016885.01.T01	MTR_3g084250	42.308	78	36	2	4	80	20	89	1.30e-13	61.6
MsG0380016885.01.T01	MTR_7g405740	40.230	87	43	2	2	87	59	137	2.40e-13	62.4
MsG0380016885.01.T01	MTR_4g072940	41.772	79	37	2	5	82	53	123	2.67e-13	61.6
MsG0380016885.01.T01	MTR_4g072330	43.038	79	36	2	3	80	17	87	3.15e-13	60.5
MsG0380016885.01.T01	MTR_4g072250	43.590	78	34	2	4	80	21	89	4.58e-13	60.1
MsG0380016885.01.T01	MTR_4g072260	42.857	77	35	2	5	80	22	90	4.79e-13	60.1
MsG0380016885.01.T01	MTR_4g072240	42.308	78	36	2	4	80	21	90	5.92e-13	59.7
MsG0380016885.01.T01	MTR_4g072230	42.857	77	33	2	4	79	21	87	7.85e-13	59.3
MsG0380016885.01.T01	MTR_4g072570	40.506	79	38	2	3	80	19	89	1.02e-12	59.3
MsG0380016885.01.T01	MTR_4g072500	45.205	73	31	2	5	76	21	85	1.08e-12	59.3
MsG0380016885.01.T01	MTR_4g072820	45.333	75	32	2	8	81	19	85	1.48e-12	58.5
MsG0380016885.01.T01	MTR_8g461420	45.833	72	30	2	5	75	21	84	1.51e-12	58.9
MsG0380016885.01.T01	MTR_4g072640	44.595	74	32	2	4	76	20	85	1.75e-12	58.5
MsG0380016885.01.T01	MTR_3g098970	40.000	70	34	1	7	76	51	112	1.82e-12	60.1
MsG0380016885.01.T01	MTR_4g072870	44.595	74	32	2	4	76	21	86	2.29e-12	58.2
MsG0380016885.01.T01	MTR_3g084240	43.421	76	34	2	2	76	18	85	2.40e-12	58.2
MsG0380016885.01.T01	MTR_4g072660	41.333	75	35	2	3	76	14	80	2.42e-12	58.2
MsG0380016885.01.T01	MTR_4g124750	40.476	84	39	2	9	92	69	141	2.46e-12	59.7
MsG0380016885.01.T01	MTR_4g072880	41.026	78	37	2	4	80	21	90	2.54e-12	58.2
MsG0380016885.01.T01	MTR_3g084170	43.421	76	34	2	2	76	18	85	2.92e-12	58.2
MsG0380016885.01.T01	MTR_4g072480	43.243	74	33	2	4	76	20	85	4.12e-12	57.8
MsG0380016885.01.T01	MTR_3g084210	45.205	73	31	2	4	75	20	84	4.74e-12	57.4
MsG0380016885.01.T01	MTR_4g072370	43.590	78	35	2	4	80	18	87	4.83e-12	57.4
MsG0380016885.01.T01	MTR_3g110045	45.833	72	30	2	5	75	80	143	5.89e-12	58.5
MsG0380016885.01.T01	MTR_3g084190	43.056	72	32	2	5	75	21	84	8.32e-12	57.0
MsG0380016885.01.T01	MTR_8g096440	43.750	80	37	1	2	81	38	109	8.63e-12	58.2
MsG0380016885.01.T01	MTR_4g072600	45.946	74	31	2	4	76	15	80	9.48e-12	56.6
MsG0380016885.01.T01	MTR_1g061570	45.455	66	26	2	9	74	19	74	9.52e-12	57.4
MsG0380016885.01.T01	MTR_3g084230	45.833	72	30	2	5	75	21	84	9.58e-12	56.6
MsG0380016885.01.T01	MTR_3g117630	39.506	81	40	2	3	82	19	91	1.07e-11	56.6
MsG0380016885.01.T01	MTR_4g072910	49.275	69	26	2	9	76	35	95	1.11e-11	56.6
MsG0380016885.01.T01	MTR_5g008370	46.377	69	28	2	9	76	25	85	1.42e-11	57.4
MsG0380016885.01.T01	MTR_4g072730	38.667	75	37	2	3	76	19	85	1.72e-11	56.2
MsG0380016885.01.T01	MTR_4g072310	41.026	78	37	2	4	80	21	90	1.72e-11	56.2
MsG0380016885.01.T01	MTR_3g084180	39.241	79	39	2	3	80	19	89	2.20e-11	55.8
MsG0380016885.01.T01	MTR_3g084150	39.744	78	38	2	3	79	19	88	2.40e-11	55.8
MsG0380016885.01.T01	MTR_3g110038	39.024	82	41	2	2	82	18	91	2.59e-11	55.5
MsG0380016885.01.T01	MTR_4g072850	40.506	79	38	2	5	82	23	93	2.62e-11	55.5
MsG0380016885.01.T01	MTR_4g072530	42.466	73	33	2	5	76	21	85	2.64e-11	55.5
MsG0380016885.01.T01	MTR_4g072620	40.541	74	35	2	4	76	20	85	3.66e-11	55.1
MsG0380016885.01.T01	MTR_4g072830	41.026	78	37	2	4	80	18	87	6.39e-11	54.7
MsG0380016885.01.T01	MTR_4g072560	39.189	74	37	2	9	81	32	98	7.36e-11	54.7
MsG0380016885.01.T01	MTR_8g096500	43.590	78	34	2	12	89	52	119	7.53e-11	55.8
MsG0380016885.01.T01	MTR_4g072860	44.595	74	32	2	4	76	21	86	8.05e-11	54.3
MsG0380016885.01.T01	MTR_7g104940	41.667	72	34	1	10	81	51	114	8.95e-11	55.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380016885.01.T01	AT5G53590	41.975	81	44	1	9	89	46	123	2.72e-17	72.4
MsG0380016885.01.T01	AT2G46690	38.947	95	52	2	2	96	17	105	5.32e-17	70.9
MsG0380016885.01.T01	AT4G00880	41.558	77	39	1	8	84	27	97	7.95e-15	65.5
MsG0380016885.01.T01	AT3G61900	41.333	75	39	1	9	83	30	99	3.16e-14	64.3
MsG0380016885.01.T01	AT4G34780	42.857	77	38	2	7	82	26	97	3.65e-13	60.8
MsG0380016885.01.T01	AT4G22620	41.026	78	41	1	3	80	73	145	1.04e-12	60.8
MsG0380016885.01.T01	AT4G34790	43.478	69	32	2	9	76	39	101	2.79e-11	55.8
MsG0380016885.01.T01	AT3G43120	42.466	73	34	1	9	81	79	143	3.15e-11	57.0
MsG0380016885.01.T01	AT5G20810	44.118	68	30	1	9	76	79	138	3.72e-11	57.0
MsG0380016885.01.T01	AT4G34800	40.000	75	39	2	3	76	15	84	5.20e-11	55.1
MsG0380016885.01.T01	AT5G20810	44.118	68	30	1	9	76	79	138	7.58e-11	56.6
MsG0380016885.01.T01	AT4G12410	37.500	72	40	1	9	80	76	142	9.49e-11	55.8

Find 28 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GACCAAGCTTATGATGTTTA+TGG	0.111493	3:-89018534	MsG0380016885.01.T01:CDS
TGCAGTTCAAGTGGGTTTAG+AGG	0.387862	3:-89018640	MsG0380016885.01.T01:CDS
CAAGAAGATGAAAGTGAAGA+AGG	0.391141	3:-89018670	MsG0380016885.01.T01:CDS
TGGTTATGGTTATGTTGGTT+AGG	0.415635	3:+89018429	None:intergenic
GACGATGCTGATGGTGGTTA+TGG	0.422891	3:+89018415	None:intergenic
GATGGTGGTTATGGTTATGT+TGG	0.451887	3:+89018424	None:intergenic
AAATTTCGAGTCAACAAGAA+TGG	0.461541	3:+89018373	None:intergenic
GGATGGTAAAGATAAGAGAT+AGG	0.469559	3:+89018576	None:intergenic
AAGAAGATGAAAGTGAAGAA+GGG	0.500937	3:-89018669	MsG0380016885.01.T01:CDS
ATGATTTCCTTCATCTTCGA+TGG	0.519321	3:-89018470	MsG0380016885.01.T01:CDS
CAATTCGCCATCGAAGATGA+AGG	0.535691	3:+89018463	None:intergenic
AGATGAAAGTGAAGAAGGGA+TGG	0.536875	3:-89018665	MsG0380016885.01.T01:CDS
AGGAAATCATCCACAGAACA+TGG	0.538192	3:+89018483	None:intergenic
AGCCATAAACATCATAAGCT+TGG	0.538249	3:+89018532	None:intergenic
TGGAAGCTTGAGAGGACCAT+TGG	0.551787	3:+89018503	None:intergenic
TCAAGAAGACGCTTGAAAAG+TGG	0.553447	3:+89018555	None:intergenic
TCTCAAGCTTCCATGTTCTG+TGG	0.559981	3:-89018493	MsG0380016885.01.T01:CDS
ACAGAACATGGAAGCTTGAG+AGG	0.564027	3:+89018495	None:intergenic
GGTTTAGAGGAAGAAGAAGA+AGG	0.564757	3:-89018627	MsG0380016885.01.T01:CDS
GGGATGGCTTGCAGTTCAAG+TGG	0.572241	3:-89018649	MsG0380016885.01.T01:CDS
GAATGGAAAATCAGAGCATG+AGG	0.588224	3:+89018390	None:intergenic
GCTTGAGAGGACCATTGGTA+TGG	0.590408	3:+89018508	None:intergenic
TTAGAGGAAGAAGAAGAAGG+TGG	0.598907	3:-89018624	MsG0380016885.01.T01:CDS
GGATGGCTTGCAGTTCAAGT+GGG	0.612179	3:-89018648	MsG0380016885.01.T01:CDS
GTTTATGGCTACCATACCAA+TGG	0.626042	3:-89018519	MsG0380016885.01.T01:CDS
GAAGACGCTTGAAAAGTGGA+TGG	0.652650	3:+89018559	None:intergenic
CATGAGGAAGACGATGCTGA+TGG	0.669865	3:+89018406	None:intergenic
GAGGAAGACGATGCTGATGG+TGG	0.699924	3:+89018409	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAGAAGATGAAAGTGAAGAA+GGG	-	Chr3:89018395-89018414	MsG0380016885.01.T01:CDS	30.0%
	AGCCATAAACATCATAAGCT+TGG	+	Chr3:89018535-89018554	None:intergenic	35.0%
	ATGATTTCCTTCATCTTCGA+TGG	-	Chr3:89018594-89018613	MsG0380016885.01.T01:CDS	35.0%
	CAAGAAGATGAAAGTGAAGA+AGG	-	Chr3:89018394-89018413	MsG0380016885.01.T01:CDS	35.0%
	GACCAAGCTTATGATGTTTA+TGG	-	Chr3:89018530-89018549	MsG0380016885.01.T01:CDS	35.0%
	GGATGGTAAAGATAAGAGAT+AGG	+	Chr3:89018491-89018510	None:intergenic	35.0%
!	TGGTTATGGTTATGTTGGTT+AGG	+	Chr3:89018638-89018657	None:intergenic	35.0%
	AGATGAAAGTGAAGAAGGGA+TGG	-	Chr3:89018399-89018418	MsG0380016885.01.T01:CDS	40.0%
	AGGAAATCATCCACAGAACA+TGG	+	Chr3:89018584-89018603	None:intergenic	40.0%
	GAATGGAAAATCAGAGCATG+AGG	+	Chr3:89018677-89018696	None:intergenic	40.0%
	GGTTTAGAGGAAGAAGAAGA+AGG	-	Chr3:89018437-89018456	MsG0380016885.01.T01:CDS	40.0%
	GTTTATGGCTACCATACCAA+TGG	-	Chr3:89018545-89018564	MsG0380016885.01.T01:CDS	40.0%
	TCAAGAAGACGCTTGAAAAG+TGG	+	Chr3:89018512-89018531	None:intergenic	40.0%
	TTAGAGGAAGAAGAAGAAGG+TGG	-	Chr3:89018440-89018459	MsG0380016885.01.T01:CDS	40.0%
!	GATGGTGGTTATGGTTATGT+TGG	+	Chr3:89018643-89018662	None:intergenic	40.0%
	ACAGAACATGGAAGCTTGAG+AGG	+	Chr3:89018572-89018591	None:intergenic	45.0%
	CAATTCGCCATCGAAGATGA+AGG	+	Chr3:89018604-89018623	None:intergenic	45.0%
	GAAGACGCTTGAAAAGTGGA+TGG	+	Chr3:89018508-89018527	None:intergenic	45.0%
	TCTCAAGCTTCCATGTTCTG+TGG	-	Chr3:89018571-89018590	MsG0380016885.01.T01:CDS	45.0%
!	TGCAGTTCAAGTGGGTTTAG+AGG	-	Chr3:89018424-89018443	MsG0380016885.01.T01:CDS	45.0%
	CATGAGGAAGACGATGCTGA+TGG	+	Chr3:89018661-89018680	None:intergenic	50.0%
	GCTTGAGAGGACCATTGGTA+TGG	+	Chr3:89018559-89018578	None:intergenic	50.0%
	TGGAAGCTTGAGAGGACCAT+TGG	+	Chr3:89018564-89018583	None:intergenic	50.0%
!	GGATGGCTTGCAGTTCAAGT+GGG	-	Chr3:89018416-89018435	MsG0380016885.01.T01:CDS	50.0%
!!	GACGATGCTGATGGTGGTTA+TGG	+	Chr3:89018652-89018671	None:intergenic	50.0%
	GAGGAAGACGATGCTGATGG+TGG	+	Chr3:89018658-89018677	None:intergenic	55.0%
!	GGGATGGCTTGCAGTTCAAG+TGG	-	Chr3:89018415-89018434	MsG0380016885.01.T01:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	89018383	89018703	89018383	ID=MsG0380016885.01;Name=MsG0380016885.01
Chr3	mRNA	89018383	89018703	89018383	ID=MsG0380016885.01.T01;Parent=MsG0380016885.01;Name=MsG0380016885.01.T01;_AED=0.42;_eAED=0.42;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|106
Chr3	exon	89018383	89018703	89018383	ID=MsG0380016885.01.T01:exon:5680;Parent=MsG0380016885.01.T01
Chr3	CDS	89018383	89018703	89018383	ID=MsG0380016885.01.T01:cds;Parent=MsG0380016885.01.T01

Gene Sequence	>MsG0380016885.01.T01 ATGCAGGAAGACAAGAAGATGAAAGTGAAGAAGGGATGGCTTGCAGTTCAAGTGGGTTTAGAGGAAGAAGAAGAAGGTGGTTCTTGCTCTCAGAAATTTGTCATACCTATCTCTTATCTTTACCATCCACTTTTCAAGCGTCTTCTTGACCAAGCTTATGATGTTTATGGCTACCATACCAATGGTCCTCTCAAGCTTCCATGTTCTGTGGATGATTTCCTTCATCTTCGATGGCGAATTGAGAAAGAATCTCCTAACCAACATAACCATAACCACCATCAGCATCGTCTTCCTCATGCTCTGATTTTCCATTCTTGTTGA
Protein sequence	>MsG0380016885.01.T01 MQEDKKMKVKKGWLAVQVGLEEEEEGGSCSQKFVIPISYLYHPLFKRLLDQAYDVYGYHTNGPLKLPCSVDDFLHLRWRIEKESPNQHNHNHHQHRLPHALIFHSC*