AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880045709.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045709.01.T01 MTR_8g077420 83.721 43 7 0 1 43 1 43 2.50e-22 83.2
MsG0880045709.01.T01 MTR_5g020540 58.537 41 17 0 3 43 2 42 2.16e-13 60.1
MsG0880045709.01.T01 MTR_8g077380 53.488 43 20 0 1 43 1 43 3.64e-13 59.7
MsG0880045709.01.T01 MTR_8g077390 53.488 43 20 0 1 43 1 43 3.64e-13 59.7
MsG0880045709.01.T01 MTR_8g077360 53.488 43 20 0 1 43 1 43 4.44e-13 59.7
MsG0880045709.01.T01 MTR_3g116720 63.889 36 13 0 3 38 14 49 5.29e-13 59.7
MsG0880045709.01.T01 MTR_1g022290 61.111 36 14 0 3 38 5 40 7.65e-12 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045709.01.T01 AT1G75250 63.889 36 13 0 3 38 10 45 8.22e-12 57.8
MsG0880045709.01.T01 AT1G75250 63.889 36 13 0 3 38 10 45 1.08e-11 56.6
MsG0880045709.01.T01 AT2G21650 64.706 34 12 0 5 38 14 47 1.84e-11 56.2
MsG0880045709.01.T01 AT1G19510 67.742 31 10 0 8 38 15 45 6.79e-11 54.7
MsG0880045709.01.T01 AT4G39250 61.765 34 13 0 5 38 14 47 8.70e-11 54.3

Find 21 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAACATTCTGCCACTTATC+TGG 0.266066 8:-63081492 None:intergenic
AATCAGTTGAAGATGTTAAA+AGG 0.300561 8:+63081528 MsG0880045709.01.T01:CDS
TTAGCATTATTTGTTCTGTA+AGG 0.355954 8:-63081608 None:intergenic
CAAACAATTTGAAAGAGCTT+TGG 0.396764 8:+63081449 MsG0880045709.01.T01:CDS
TGCGCCTAACATCTTCTTTG+AGG 0.404699 8:-63081562 None:intergenic
TAATTCCTTCTTTCACTGGC+AGG 0.407190 8:+63081872 MsG0880045709.01.T01:CDS
TAACTGAGGCCATGGTCTTA+AGG 0.410966 8:+63081631 MsG0880045709.01.T01:CDS
TAGCATTATTTGTTCTGTAA+GGG 0.498967 8:-63081607 None:intergenic
TGGGATCAACTTGGACTCCA+AGG 0.513093 8:+63081421 MsG0880045709.01.T01:CDS
AATGCTAATAACTGAGGCCA+TGG 0.521264 8:+63081623 MsG0880045709.01.T01:CDS
AGATCCTCAAAGAAGATGTT+AGG 0.522074 8:+63081558 MsG0880045709.01.T01:CDS
TTCTTGAAATGGGATCAACT+TGG 0.536988 8:+63081412 None:intergenic
GAGAACCTGCCAGTGAAAGA+AGG 0.538421 8:-63081877 None:intergenic
CAACTGATTTCCCACCATGT+TGG 0.546180 8:-63081514 None:intergenic
ATAGAGATTCTCCAGATAAG+TGG 0.553229 8:+63081481 MsG0880045709.01.T01:CDS
GATGTTAGGCGCATTGAGCA+TGG 0.583089 8:+63081572 MsG0880045709.01.T01:CDS
GTGGCAGAATGTTGCCAACA+TGG 0.629175 8:+63081500 MsG0880045709.01.T01:CDS
ACAAATAATGCTAATAACTG+AGG 0.631984 8:+63081617 MsG0880045709.01.T01:CDS
AATCATATACCTTAAGACCA+TGG 0.641285 8:-63081640 None:intergenic
CAGAATGTTGCCAACATGGT+GGG 0.684607 8:+63081504 MsG0880045709.01.T01:CDS
GCAGAATGTTGCCAACATGG+TGG 0.706284 8:+63081503 MsG0880045709.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAAAAATGTGGGAAAA+AGG - Chr8:63081668-63081687 None:intergenic 20.0%
!! AGATGTGAATAAAAAAATGT+GGG - Chr8:63081753-63081772 None:intergenic 20.0%
!! TAGATGTGAATAAAAAAATG+TGG - Chr8:63081754-63081773 None:intergenic 20.0%
! AATCAGTTGAAGATGTTAAA+AGG + Chr8:63081528-63081547 MsG0880045709.01.T01:CDS 25.0%
! ACAAATAATGCTAATAACTG+AGG + Chr8:63081617-63081636 MsG0880045709.01.T01:CDS 25.0%
! GGATGTGAATAAAAAAATGT+GGG - Chr8:63081675-63081694 None:intergenic 25.0%
! TAGCATTATTTGTTCTGTAA+GGG - Chr8:63081610-63081629 None:intergenic 25.0%
! TTAGCATTATTTGTTCTGTA+AGG - Chr8:63081611-63081630 None:intergenic 25.0%
AATCATATACCTTAAGACCA+TGG - Chr8:63081643-63081662 None:intergenic 30.0%
CAAACAATTTGAAAGAGCTT+TGG + Chr8:63081449-63081468 MsG0880045709.01.T01:CDS 30.0%
CGGATGTGAATAAAAAAATG+TGG - Chr8:63081676-63081695 None:intergenic 30.0%
! ACTTGAGTCTTCAATTTTGT+TGG - Chr8:63081913-63081932 None:intergenic 30.0%
! TCTTCAATTTTGTTGGATTC+TGG - Chr8:63081906-63081925 None:intergenic 30.0%
!! CTTTTAATTCCTTCTTTCAC+TGG + Chr8:63081868-63081887 MsG0880045709.01.T01:CDS 30.0%
!!! TCAAATTGTTTGTTTTGCCT+TGG - Chr8:63081441-63081460 None:intergenic 30.0%
AGATCCTCAAAGAAGATGTT+AGG + Chr8:63081558-63081577 MsG0880045709.01.T01:CDS 35.0%
AGATTCTGTACAAGATTGCA+CGG - Chr8:63081696-63081715 None:intergenic 35.0%
ATAGAGATTCTCCAGATAAG+TGG + Chr8:63081481-63081500 MsG0880045709.01.T01:CDS 35.0%
TTTAGTATAGCTACAGCCTT+TGG + Chr8:63081803-63081822 MsG0880045709.01.T01:intron 35.0%
AATGCTAATAACTGAGGCCA+TGG + Chr8:63081623-63081642 MsG0880045709.01.T01:CDS 40.0%
AGCATATATACAGCAGCCAA+AGG - Chr8:63081822-63081841 None:intergenic 40.0%
TAATTCCTTCTTTCACTGGC+AGG + Chr8:63081872-63081891 MsG0880045709.01.T01:CDS 40.0%
CAGAATGTTGCCAACATGGT+GGG + Chr8:63081504-63081523 MsG0880045709.01.T01:CDS 45.0%
TGCGCCTAACATCTTCTTTG+AGG - Chr8:63081565-63081584 None:intergenic 45.0%
! CAACTGATTTCCCACCATGT+TGG - Chr8:63081517-63081536 None:intergenic 45.0%
! GCAACATTCTGCCACTTATC+TGG - Chr8:63081495-63081514 None:intergenic 45.0%
! TAACTGAGGCCATGGTCTTA+AGG + Chr8:63081631-63081650 MsG0880045709.01.T01:CDS 45.0%
GAGAACCTGCCAGTGAAAGA+AGG - Chr8:63081880-63081899 None:intergenic 50.0%
GATGTTAGGCGCATTGAGCA+TGG + Chr8:63081572-63081591 MsG0880045709.01.T01:CDS 50.0%
GCAGAATGTTGCCAACATGG+TGG + Chr8:63081503-63081522 MsG0880045709.01.T01:CDS 50.0%
GTGGCAGAATGTTGCCAACA+TGG + Chr8:63081500-63081519 MsG0880045709.01.T01:CDS 50.0%
TGGGATCAACTTGGACTCCA+AGG + Chr8:63081421-63081440 MsG0880045709.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr8 gene 63081420 63081944 63081420 ID=MsG0880045709.01;Name=MsG0880045709.01
Chr8 mRNA 63081420 63081944 63081420 ID=MsG0880045709.01.T01;Parent=MsG0880045709.01;Name=MsG0880045709.01.T01;_AED=0.50;_eAED=0.53;_QI=0|0|0|1|0|0|2|0|105
Chr8 exon 63081420 63081652 63081420 ID=MsG0880045709.01.T01:exon:24186;Parent=MsG0880045709.01.T01
Chr8 exon 63081860 63081944 63081860 ID=MsG0880045709.01.T01:exon:24187;Parent=MsG0880045709.01.T01
Chr8 CDS 63081420 63081652 63081420 ID=MsG0880045709.01.T01:cds;Parent=MsG0880045709.01.T01
Chr8 CDS 63081860 63081944 63081860 ID=MsG0880045709.01.T01:cds;Parent=MsG0880045709.01.T01
Gene Sequence

>MsG0880045709.01.T01

ATGGGATCAACTTGGACTCCAAGGCAAAACAAACAATTTGAAAGAGCTTTGGCTATATATGATAGAGATTCTCCAGATAAGTGGCAGAATGTTGCCAACATGGTGGGAAATCAGTTGAAGATGTTAAAAGGCATTATGAGATCCTCAAAGAAGATGTTAGGCGCATTGAGCATGGTCAAGTTGCATTCCCTTACAGAACAAATAATGCTAATAACTGAGGCCATGGTCTTAAGTGCACTGACTTTTAATTCCTTCTTTCACTGGCAGGTTCTCAATCCAGAATCCAACAAAATTGAAGACTCAAGTATCCATAGTTAA

Protein sequence

>MsG0880045709.01.T01

MGSTWTPRQNKQFERALAIYDRDSPDKWQNVANMVGNQLKMLKGIMRSSKKMLGALSMVKLHSLTEQIMLITEAMVLSALTFNSFFHWQVLNPESNKIEDSSIHS*