AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380016986.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016986.01.T01 MTR_3g098730 89.076 238 6 3 1 219 151 387 8.31e-128 372
MsG0380016986.01.T01 MTR_8g032570 37.674 215 107 5 1 192 163 373 6.57e-30 116
MsG0380016986.01.T01 MTR_8g032570 37.674 215 107 5 1 192 186 396 6.84e-30 116
MsG0380016986.01.T01 MTR_7g076220 33.962 212 118 2 1 192 163 372 1.69e-28 112
MsG0380016986.01.T01 MTR_4g091320 28.837 215 128 4 1 192 164 376 7.40e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016986.01.T01 AT5G50900 62.605 238 70 2 1 219 153 390 1.15e-91 279
MsG0380016986.01.T01 AT2G45720 34.579 214 114 4 1 192 158 367 5.95e-26 105
MsG0380016986.01.T01 AT2G45720 34.579 214 114 4 1 192 158 367 5.95e-26 105
MsG0380016986.01.T01 AT2G45720 34.579 214 114 4 1 192 158 367 5.95e-26 105
MsG0380016986.01.T01 AT2G45720 34.579 214 114 4 1 192 158 367 5.95e-26 105
MsG0380016986.01.T01 AT2G45720 34.579 214 114 4 1 192 158 367 5.95e-26 105
MsG0380016986.01.T01 AT1G01830 37.209 215 108 5 1 192 176 386 2.23e-24 100
MsG0380016986.01.T01 AT1G01830 37.209 215 108 5 1 192 176 386 2.23e-24 100
MsG0380016986.01.T01 AT1G01830 37.209 215 108 5 1 192 176 386 2.23e-24 100
MsG0380016986.01.T01 AT1G61350 28.846 208 122 5 1 185 156 360 7.85e-14 70.1

Find 60 sgRNAs with CRISPR-Local

Find 73 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAATCATTTGGTTAGGGTTT+TGG 0.180873 3:-90313528 MsG0380016986.01.T01:CDS
GGTTGAAATGTTGAGGTATT+TGG 0.223216 3:-90313101 MsG0380016986.01.T01:CDS
TTGAGTGTTTGAAGAATTAT+TGG 0.266104 3:-90313159 MsG0380016986.01.T01:CDS
ATTGCAGTGGCGCGTGATTC+TGG 0.283476 3:+90313749 None:intergenic
GAAAGCTTGTATTGCGCTTC+AGG 0.297814 3:-90313480 MsG0380016986.01.T01:CDS
GGATGAGAGTATGAGGGTTT+TGG 0.343750 3:-90313200 MsG0380016986.01.T01:CDS
TGTTGTTGAATCATTTGGTT+AGG 0.343807 3:-90313535 MsG0380016986.01.T01:CDS
TTGTTAGGGTTGGCTTCGTC+TGG 0.344793 3:-90313276 MsG0380016986.01.T01:intron
GTTGTTGTTGTTGAATCATT+TGG 0.357475 3:-90313540 MsG0380016986.01.T01:CDS
GGTGGAATTTCGTCTCTGTT+AGG 0.358232 3:-90313413 MsG0380016986.01.T01:CDS
ACCAACTAAACCTCATCAAT+TGG 0.390014 3:+90313067 None:intergenic
TTTGAAGAATTATTGGGATT+CGG 0.396056 3:-90313152 MsG0380016986.01.T01:CDS
TTGTGTGGCAAATTTGATTT+CGG 0.412915 3:-90313224 MsG0380016986.01.T01:CDS
AGGGTTGGCTTCGTCTGGAA+CGG 0.423372 3:-90313271 MsG0380016986.01.T01:CDS
GTGTAGTTCCGGTGCTGGTT+CGG 0.423585 3:-90313664 MsG0380016986.01.T01:CDS
GGGTTGGCTTCGTCTGGAAC+GGG 0.424514 3:-90313270 MsG0380016986.01.T01:CDS
TTTCGGAGGATGAGAGTATG+AGG 0.432008 3:-90313207 MsG0380016986.01.T01:CDS
CTCATCGCACGGTTGCAAAT+CGG 0.432560 3:-90313776 None:intergenic
CTCCTTCATATCAGAGGAGC+TGG 0.435157 3:+90313631 None:intergenic
GTGGAATTTCGTCTCTGTTA+GGG 0.438416 3:-90313412 MsG0380016986.01.T01:CDS
GCAAGGTGTAGTTCCGGTGC+TGG 0.439174 3:-90313669 MsG0380016986.01.T01:CDS
GTTGTTGAATCATTTGGTTA+GGG 0.469282 3:-90313534 MsG0380016986.01.T01:CDS
TGAGTGTTTGAAGAATTATT+GGG 0.492153 3:-90313158 MsG0380016986.01.T01:CDS
TGATTCAGAGTCTTGAAGTA+GGG 0.493354 3:-90313123 MsG0380016986.01.T01:CDS
AGGGACAATGCTAGAGCGAT+TGG 0.504635 3:-90313443 MsG0380016986.01.T01:CDS
TTCCAGCTCCTCTGATATGA+AGG 0.514211 3:-90313633 MsG0380016986.01.T01:CDS
GCTGCATAACCTTGTGAACC+TGG 0.516453 3:+90313371 None:intergenic
TGCTATTTCGAGAGTTTCCA+CGG 0.517769 3:-90313597 MsG0380016986.01.T01:CDS
TTCAGGCGTTGAGTTTGACT+AGG 0.520825 3:-90313463 MsG0380016986.01.T01:CDS
CGTCGCGCAAGGTGTAGTTC+CGG 0.521124 3:-90313675 MsG0380016986.01.T01:CDS
ATTTGTCAAGGCGGAACACC+AGG 0.523455 3:-90313389 MsG0380016986.01.T01:CDS
ATGATTCAGAGTCTTGAAGT+AGG 0.530753 3:-90313124 MsG0380016986.01.T01:CDS
TGGCTTCGTCTGGAACGGGT+TGG 0.533465 3:-90313266 MsG0380016986.01.T01:CDS
TTCGGAGGATGAGAGTATGA+GGG 0.538974 3:-90313206 MsG0380016986.01.T01:CDS
TTGAGGTATTTGGCTATGAC+TGG 0.545352 3:-90313091 MsG0380016986.01.T01:CDS
AAGTAGGGGTTGAAATGTTG+AGG 0.549591 3:-90313108 MsG0380016986.01.T01:CDS
AAGAATAATTTACTTGCGGA+GGG 0.550763 3:-90313563 MsG0380016986.01.T01:CDS
TCGAGTAACCGAACCAGCAC+CGG 0.554153 3:+90313656 None:intergenic
TCAGGCGTTGAGTTTGACTA+GGG 0.566469 3:-90313462 MsG0380016986.01.T01:CDS
TTGGCACGAGAAAATGCGAT+TGG 0.567725 3:-90313247 MsG0380016986.01.T01:CDS
AGAGTTTCCACGGTGGAGAG+TGG 0.573107 3:-90313587 MsG0380016986.01.T01:CDS
GAGTTTCCACGGTGGAGAGT+GGG 0.574172 3:-90313586 MsG0380016986.01.T01:CDS
GAAGAATAATTTACTTGCGG+AGG 0.596771 3:-90313564 MsG0380016986.01.T01:CDS
TCTCTGTTAGGGATTTGTCA+AGG 0.604955 3:-90313401 MsG0380016986.01.T01:CDS
GGCGGAACACCAGGTTCACA+AGG 0.606227 3:-90313380 MsG0380016986.01.T01:CDS
TTCACAAGGTTATGCAGCAG+CGG 0.614157 3:-90313366 MsG0380016986.01.T01:CDS
GATTCAGAGTCTTGAAGTAG+GGG 0.614676 3:-90313122 MsG0380016986.01.T01:CDS
TGGGAAGAATAATTTACTTG+CGG 0.619324 3:-90313567 MsG0380016986.01.T01:CDS
CGGAACTACACCTTGCGCGA+CGG 0.625906 3:+90313676 None:intergenic
GTTACAATAGCCGTCGCGCA+AGG 0.626258 3:-90313686 MsG0380016986.01.T01:CDS
TGTGGCAAATTTGATTTCGG+AGG 0.627323 3:-90313221 MsG0380016986.01.T01:CDS
AGAAAATGCGATTGGTTGTG+TGG 0.632842 3:-90313239 MsG0380016986.01.T01:CDS
GCTAGAGCGATTGGATCTAG+AGG 0.633516 3:-90313434 MsG0380016986.01.T01:CDS
AGAATAATTTACTTGCGGAG+GGG 0.633658 3:-90313562 MsG0380016986.01.T01:CDS
AGAGCGATTGGATCTAGAGG+TGG 0.653759 3:-90313431 MsG0380016986.01.T01:CDS
TATGACTGGTCCAATTGATG+AGG 0.665742 3:-90313077 MsG0380016986.01.T01:CDS
TAAGAGTGAGTCGATTGCAG+TGG 0.671105 3:+90313736 None:intergenic
ATTCTTCCCACTCTCCACCG+TGG 0.707457 3:+90313580 None:intergenic
CTGTTAGGGATTTGTCAAGG+CGG 0.747669 3:-90313398 MsG0380016986.01.T01:CDS
TATTTCGAGAGTTTCCACGG+TGG 0.826858 3:-90313594 MsG0380016986.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ATGAAATTAAGAGAATTTCG+TGG - Chr3:90313517-90313536 MsG0380016986.01.T01:CDS 25.0%
! TGAGTGTTTGAAGAATTATT+GGG - Chr3:90313678-90313697 MsG0380016986.01.T01:CDS 25.0%
! TTGAGTGTTTGAAGAATTAT+TGG - Chr3:90313677-90313696 MsG0380016986.01.T01:CDS 25.0%
! TTTGAAGAATTATTGGGATT+CGG - Chr3:90313684-90313703 MsG0380016986.01.T01:CDS 25.0%
AAATTAAGAGAATTTCGTGG+AGG - Chr3:90313520-90313539 MsG0380016986.01.T01:CDS 30.0%
AAGAATAATTTACTTGCGGA+GGG - Chr3:90313273-90313292 MsG0380016986.01.T01:intron 30.0%
GTTGTTGAATCATTTGGTTA+GGG - Chr3:90313302-90313321 MsG0380016986.01.T01:intron 30.0%
TGGGAAGAATAATTTACTTG+CGG - Chr3:90313269-90313288 MsG0380016986.01.T01:CDS 30.0%
TGTTGTTGAATCATTTGGTT+AGG - Chr3:90313301-90313320 MsG0380016986.01.T01:intron 30.0%
! GTTGTTGTTGTTGAATCATT+TGG - Chr3:90313296-90313315 MsG0380016986.01.T01:intron 30.0%
! TTGTGTGGCAAATTTGATTT+CGG - Chr3:90313612-90313631 MsG0380016986.01.T01:CDS 30.0%
!! AATGCGGTTATTGTTTTGTT+AGG - Chr3:90313545-90313564 MsG0380016986.01.T01:CDS 30.0%
!! ATGCGGTTATTGTTTTGTTA+GGG - Chr3:90313546-90313565 MsG0380016986.01.T01:CDS 30.0%
AGAATAATTTACTTGCGGAG+GGG - Chr3:90313274-90313293 MsG0380016986.01.T01:intron 35.0%
ATGATTCAGAGTCTTGAAGT+AGG - Chr3:90313712-90313731 MsG0380016986.01.T01:CDS 35.0%
GAAGAATAATTTACTTGCGG+AGG - Chr3:90313272-90313291 MsG0380016986.01.T01:CDS 35.0%
TGATTCAGAGTCTTGAAGTA+GGG - Chr3:90313713-90313732 MsG0380016986.01.T01:CDS 35.0%
! GAATCATTTGGTTAGGGTTT+TGG - Chr3:90313308-90313327 MsG0380016986.01.T01:intron 35.0%
! GGTTGAAATGTTGAGGTATT+TGG - Chr3:90313735-90313754 MsG0380016986.01.T01:CDS 35.0%
! TGTTTTCTCCTTCATATCAG+AGG + Chr3:90313214-90313233 None:intergenic 35.0%
!! GGTTATTGTTTTGTTAGGGT+TGG - Chr3:90313550-90313569 MsG0380016986.01.T01:CDS 35.0%
!!! TTTGGTTAGGGTTTTGGATT+CGG - Chr3:90313314-90313333 MsG0380016986.01.T01:intron 35.0%
AAGTAGGGGTTGAAATGTTG+AGG - Chr3:90313728-90313747 MsG0380016986.01.T01:CDS 40.0%
GATTCAGAGTCTTGAAGTAG+GGG - Chr3:90313714-90313733 MsG0380016986.01.T01:CDS 40.0%
GTGGAATTTCGTCTCTGTTA+GGG - Chr3:90313424-90313443 MsG0380016986.01.T01:CDS 40.0%
TCTCTGTTAGGGATTTGTCA+AGG - Chr3:90313435-90313454 MsG0380016986.01.T01:CDS 40.0%
! AGAAAATGCGATTGGTTGTG+TGG - Chr3:90313597-90313616 MsG0380016986.01.T01:CDS 40.0%
! TATGACTGGTCCAATTGATG+AGG - Chr3:90313759-90313778 MsG0380016986.01.T01:CDS 40.0%
! TGTGGCAAATTTGATTTCGG+AGG - Chr3:90313615-90313634 MsG0380016986.01.T01:CDS 40.0%
! TTGAGGTATTTGGCTATGAC+TGG - Chr3:90313745-90313764 MsG0380016986.01.T01:CDS 40.0%
!! TGCTATTTCGAGAGTTTCCA+CGG - Chr3:90313239-90313258 MsG0380016986.01.T01:CDS 40.0%
!!! TTGGTTAGGGTTTTGGATTC+GGG - Chr3:90313315-90313334 MsG0380016986.01.T01:intron 40.0%
CTGTTAGGGATTTGTCAAGG+CGG - Chr3:90313438-90313457 MsG0380016986.01.T01:CDS 45.0%
GAAAGCTTGTATTGCGCTTC+AGG - Chr3:90313356-90313375 MsG0380016986.01.T01:CDS 45.0%
GAATTTCGTGGAGGAGAATG+CGG - Chr3:90313529-90313548 MsG0380016986.01.T01:CDS 45.0%
GGTGGAATTTCGTCTCTGTT+AGG - Chr3:90313423-90313442 MsG0380016986.01.T01:CDS 45.0%
TAAGAGTGAGTCGATTGCAG+TGG + Chr3:90313103-90313122 None:intergenic 45.0%
TATTTCGAGAGTTTCCACGG+TGG - Chr3:90313242-90313261 MsG0380016986.01.T01:CDS 45.0%
TTCACAAGGTTATGCAGCAG+CGG - Chr3:90313470-90313489 MsG0380016986.01.T01:CDS 45.0%
TTCCAGCTCCTCTGATATGA+AGG - Chr3:90313203-90313222 MsG0380016986.01.T01:CDS 45.0%
TTGGCACGAGAAAATGCGAT+TGG - Chr3:90313589-90313608 MsG0380016986.01.T01:CDS 45.0%
! TTCGGAGGATGAGAGTATGA+GGG - Chr3:90313630-90313649 MsG0380016986.01.T01:CDS 45.0%
! TTTCGGAGGATGAGAGTATG+AGG - Chr3:90313629-90313648 MsG0380016986.01.T01:CDS 45.0%
!! GGATGAGAGTATGAGGGTTT+TGG - Chr3:90313636-90313655 MsG0380016986.01.T01:CDS 45.0%
!! TCAGGCGTTGAGTTTGACTA+GGG - Chr3:90313374-90313393 MsG0380016986.01.T01:CDS 45.0%
!! TTCAGGCGTTGAGTTTGACT+AGG - Chr3:90313373-90313392 MsG0380016986.01.T01:CDS 45.0%
!!! TATGAGGGTTTTGGCTGTGA+AGG - Chr3:90313645-90313664 MsG0380016986.01.T01:CDS 45.0%
AGAGCGATTGGATCTAGAGG+TGG - Chr3:90313405-90313424 MsG0380016986.01.T01:CDS 50.0%
AGGGACAATGCTAGAGCGAT+TGG - Chr3:90313393-90313412 MsG0380016986.01.T01:CDS 50.0%
ATTTGTCAAGGCGGAACACC+AGG - Chr3:90313447-90313466 MsG0380016986.01.T01:CDS 50.0%
CTCCTTCATATCAGAGGAGC+TGG + Chr3:90313208-90313227 None:intergenic 50.0%
GCTAGAGCGATTGGATCTAG+AGG - Chr3:90313402-90313421 MsG0380016986.01.T01:CDS 50.0%
GCTGCATAACCTTGTGAACC+TGG + Chr3:90313468-90313487 None:intergenic 50.0%
! GTTATGCAGCAGCGGTTTTG+AGG - Chr3:90313478-90313497 MsG0380016986.01.T01:CDS 50.0%
!! TTGTTAGGGTTGGCTTCGTC+TGG - Chr3:90313560-90313579 MsG0380016986.01.T01:CDS 50.0%
!!! AGCAGCGGTTTTGAGGAATC+TGG - Chr3:90313485-90313504 MsG0380016986.01.T01:CDS 50.0%
!!! AGGGTTTTGGATTCGGGAAG+TGG - Chr3:90313321-90313340 MsG0380016986.01.T01:intron 50.0%
!!! AGGGTTTTGGCTGTGAAGGA+AGG - Chr3:90313649-90313668 MsG0380016986.01.T01:CDS 50.0%
!!! GTTTTGGCTGTGAAGGAAGG+TGG - Chr3:90313652-90313671 MsG0380016986.01.T01:CDS 50.0%
AGAGTTTCCACGGTGGAGAG+TGG - Chr3:90313249-90313268 MsG0380016986.01.T01:CDS 55.0%
ATTCTTCCCACTCTCCACCG+TGG + Chr3:90313259-90313278 None:intergenic 55.0%
ATTGCAGTGGCGCGTGATTC+TGG + Chr3:90313090-90313109 None:intergenic 55.0%
GAGTTTCCACGGTGGAGAGT+GGG - Chr3:90313250-90313269 MsG0380016986.01.T01:CDS 55.0%
GTTACAATAGCCGTCGCGCA+AGG - Chr3:90313150-90313169 MsG0380016986.01.T01:CDS 55.0%
TCGAGTAACCGAACCAGCAC+CGG + Chr3:90313183-90313202 None:intergenic 55.0%
!! AGGGTTGGCTTCGTCTGGAA+CGG - Chr3:90313565-90313584 MsG0380016986.01.T01:CDS 55.0%
!! GTGTAGTTCCGGTGCTGGTT+CGG - Chr3:90313172-90313191 MsG0380016986.01.T01:CDS 55.0%
CGGAACTACACCTTGCGCGA+CGG + Chr3:90313163-90313182 None:intergenic 60.0%
CGTCGCGCAAGGTGTAGTTC+CGG - Chr3:90313161-90313180 MsG0380016986.01.T01:CDS 60.0%
GCAAGGTGTAGTTCCGGTGC+TGG - Chr3:90313167-90313186 MsG0380016986.01.T01:CDS 60.0%
GGCGGAACACCAGGTTCACA+AGG - Chr3:90313456-90313475 MsG0380016986.01.T01:CDS 60.0%
! TGGCTTCGTCTGGAACGGGT+TGG - Chr3:90313570-90313589 MsG0380016986.01.T01:CDS 60.0%
!! GGGTTGGCTTCGTCTGGAAC+GGG - Chr3:90313566-90313585 MsG0380016986.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr3 gene 90313072 90313786 90313072 ID=MsG0380016986.01;Name=MsG0380016986.01
Chr3 mRNA 90313072 90313786 90313072 ID=MsG0380016986.01.T01;Parent=MsG0380016986.01;Name=MsG0380016986.01.T01;_AED=0.37;_eAED=0.39;_QI=0|0|0|1|1|1|2|0|219
Chr3 exon 90313347 90313786 90313347 ID=MsG0380016986.01.T01:exon:5786;Parent=MsG0380016986.01.T01
Chr3 exon 90313072 90313291 90313072 ID=MsG0380016986.01.T01:exon:5785;Parent=MsG0380016986.01.T01
Chr3 CDS 90313347 90313786 90313347 ID=MsG0380016986.01.T01:cds;Parent=MsG0380016986.01.T01
Chr3 CDS 90313072 90313291 90313072 ID=MsG0380016986.01.T01:cds;Parent=MsG0380016986.01.T01
Gene Sequence

>MsG0380016986.01.T01

TTGCAAATCGGCTCACCAGAATCACGCGCCACTGCAATCGACTCACTCTTATCTCTTCTCAATGAAGATGATAAAAACGTTACAATAGCCGTCGCGCAAGGTGTAGTTCCGGTGCTGGTTCGGTTACTCGATTCCAGCTCCTCTGATATGAAGGAGAAAACAGTTGCTGCTATTTCGAGAGTTTCCACGGTGGAGAGTGGGAAGAATAATTTACTTGCGGAGGGGTTGTTGTTGTTGAATCATTTGGTTAGGGTTTTGGATTCGGGAAGTGGTTTAGCTATAGAGAAAGCTTGTATTGCGCTTCAGGCGTTGAGTTTGACTAGGGACAATGCTAGAGCGATTGGATCTAGAGGTGGAATTTCGTCTCTGTTAGGGATTTGTCAAGGCGGAACACCAGGTTCACAAGGTTATGCAGCAGCGGTTTTGAGGAATCTGGCTAAGGTTGGCTTCGTCTGGAACGGGTTGGCACGAGAAAATGCGATTGGTTGTGTGGCAAATTTGATTTCGGAGGATGAGAGTATGAGGGTTTTGGCTGTGAAGGAAGGTGGTGTTGAGTGTTTGAAGAATTATTGGGATTCGGTTACGATGATTCAGAGTCTTGAAGTAGGGGTTGAAATGTTGAGGTATTTGGCTATGACTGGTCCAATTGATGAGGTTTAG

Protein sequence

>MsG0380016986.01.T01

LQIGSPESRATAIDSLLSLLNEDDKNVTIAVAQGVVPVLVRLLDSSSSDMKEKTVAAISRVSTVESGKNNLLAEGLLLLNHLVRVLDSGSGLAIEKACIALQALSLTRDNARAIGSRGGISSLLGICQGGTPGSQGYAAAVLRNLAKVGFVWNGLARENAIGCVANLISEDESMRVLAVKEGGVECLKNYWDSVTMIQSLEVGVEMLRYLAMTGPIDEV*