Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017398.01.T01 | AES73603.1 | 95.039 | 383 | 15 | 2 | 1 | 379 | 1 | 383 | 0 | 721 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017398.01.T01 | G7JAG4 | 95.039 | 383 | 15 | 2 | 1 | 379 | 1 | 383 | 0.0 | 721 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000595.01 | MsG0380017398.01 | 0.817714 | 2.856910e-52 | 2.096366e-49 |
| MsG0380016753.01 | MsG0380017398.01 | 0.805747 | 1.149939e-49 | 6.132663e-47 |
| MsG0380017398.01 | MsG0780041767.01 | 0.803137 | 4.023819e-49 | 2.006463e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017398.01.T01 | MTR_3g106630 | 95.039 | 383 | 15 | 2 | 1 | 379 | 1 | 383 | 0.0 | 721 |
| MsG0380017398.01.T01 | MTR_4g115690 | 43.548 | 372 | 177 | 6 | 10 | 379 | 20 | 360 | 3.43e-91 | 278 |
| MsG0380017398.01.T01 | MTR_4g014150 | 34.496 | 258 | 120 | 6 | 36 | 290 | 36 | 247 | 4.69e-38 | 137 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 62 sgRNAs with CRISPR-Local
Find 82 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAACCAACATACGTTCTAAA+AGG | 0.193644 | 3:+95807844 | None:intergenic |
| GGAATGATTCCTAAGATTAT+TGG | 0.204241 | 3:-95807612 | MsG0380017398.01.T01:CDS |
| TTTGCAATACAAGTGGAAAT+TGG | 0.242240 | 3:-95807360 | MsG0380017398.01.T01:CDS |
| TGAGAGAGATTGTAAAGATT+TGG | 0.261084 | 3:+95808164 | None:intergenic |
| TTTGATGGGTCCAACGATTA+AGG | 0.295162 | 3:-95807730 | MsG0380017398.01.T01:CDS |
| AGTCTGAATTTCGGATTCTA+AGG | 0.319480 | 3:+95808025 | None:intergenic |
| TTGCAATACAAGTGGAAATT+GGG | 0.326426 | 3:-95807359 | MsG0380017398.01.T01:CDS |
| AACAATAAACCAATAATCTT+AGG | 0.326765 | 3:+95807603 | None:intergenic |
| GAGATTAGAGAAAAGAGGTT+AGG | 0.345793 | 3:-95807426 | MsG0380017398.01.T01:CDS |
| AGCTATGGCTATAGCGGATT+TGG | 0.357030 | 3:-95807787 | MsG0380017398.01.T01:CDS |
| GTCTAAATAAGTCTGAATTT+CGG | 0.358799 | 3:+95808016 | None:intergenic |
| AGATTAGAGAAAAGAGGTTA+GGG | 0.375202 | 3:-95807425 | MsG0380017398.01.T01:CDS |
| GTGTCTTCGTGTTGCGGCTT+TGG | 0.376492 | 3:-95807568 | MsG0380017398.01.T01:CDS |
| GGCTTCGGAGTGTTCTATAA+AGG | 0.379168 | 3:-95807694 | MsG0380017398.01.T01:CDS |
| CGGTGCTTAGAAGGTTCATC+AGG | 0.395543 | 3:+95808341 | None:intergenic |
| AAATCTTGTATGAAATGATT+TGG | 0.410065 | 3:+95808063 | None:intergenic |
| GGCTTTGATAGCAATGGCTT+CGG | 0.418058 | 3:-95807709 | MsG0380017398.01.T01:CDS |
| CTGTTAGTGCTGAGATATTG+TGG | 0.420831 | 3:-95807245 | MsG0380017398.01.T01:CDS |
| GTAAGAACACGGTGCTTAGA+AGG | 0.430132 | 3:+95808332 | None:intergenic |
| GATAAAATTTCATCAACTAA+AGG | 0.439999 | 3:+95807639 | None:intergenic |
| TGTTAGTGCTGAGATATTGT+GGG | 0.445099 | 3:-95807244 | MsG0380017398.01.T01:CDS |
| ATTAGATGAGAAGATAGTTA+TGG | 0.445440 | 3:+95808187 | None:intergenic |
| TAAAACCTTGATCGAAAACT+TGG | 0.445674 | 3:-95808126 | MsG0380017398.01.T01:CDS |
| ACAGAACAACGAGGATGAAC+AGG | 0.447175 | 3:-95807955 | MsG0380017398.01.T01:CDS |
| CAACGAGGATGAACAGGTTA+AGG | 0.466181 | 3:-95807949 | MsG0380017398.01.T01:CDS |
| ATCTTTGGAAGCTTGTTTGA+GGG | 0.467224 | 3:+95808298 | None:intergenic |
| TTGAACCCTTTACTTCGAAA+AGG | 0.468750 | 3:-95807824 | MsG0380017398.01.T01:CDS |
| GATCTTTGGAAGCTTGTTTG+AGG | 0.477620 | 3:+95808297 | None:intergenic |
| TAGAGAAAAGAGGTTAGGGG+TGG | 0.485970 | 3:-95807421 | MsG0380017398.01.T01:CDS |
| ATGAAATGATTTGGCGTTGA+AGG | 0.487135 | 3:+95808072 | None:intergenic |
| AAGCTCTAAAAGAGCTTCAA+TGG | 0.491502 | 3:+95808220 | None:intergenic |
| TGAACCCTTTACTTCGAAAA+GGG | 0.497904 | 3:-95807823 | MsG0380017398.01.T01:CDS |
| TGGAAGAGATTAGAGAAAAG+AGG | 0.504380 | 3:-95807431 | MsG0380017398.01.T01:CDS |
| GTTTGAACAGAGGTTATTGT+CGG | 0.514496 | 3:-95807916 | MsG0380017398.01.T01:CDS |
| AATGATTTGGCGTTGAAGGA+TGG | 0.515159 | 3:+95808076 | None:intergenic |
| TTCGAGATTTGCAATACAAG+TGG | 0.518226 | 3:-95807367 | MsG0380017398.01.T01:CDS |
| AACCAACATACGTTCTAAAA+GGG | 0.519907 | 3:+95807845 | None:intergenic |
| GAAGAGAAAAGTGAAAGGGT+TGG | 0.521905 | 3:+95808262 | None:intergenic |
| GCTATCAAAGCCTTAATCGT+TGG | 0.522227 | 3:+95807720 | None:intergenic |
| AAAAGAGGATTTATGTGATG+TGG | 0.525491 | 3:-95807451 | MsG0380017398.01.T01:CDS |
| TGATTTGTGTCTTCGTGTTG+CGG | 0.528822 | 3:-95807574 | MsG0380017398.01.T01:CDS |
| AGCAATGTTACAGCAAGAGT+TGG | 0.539505 | 3:-95807499 | MsG0380017398.01.T01:CDS |
| GAAATTGGAGATACTGAGAT+TGG | 0.551471 | 3:-95807304 | MsG0380017398.01.T01:CDS |
| GTTCTTGCAGTGTCTTCACA+AGG | 0.552523 | 3:+95807976 | None:intergenic |
| GGAGATACTGAGATTGGTGA+AGG | 0.553572 | 3:-95807298 | MsG0380017398.01.T01:CDS |
| TTTGAACAGAGGTTATTGTC+GGG | 0.555301 | 3:-95807915 | MsG0380017398.01.T01:CDS |
| TGCAATACAAGTGGAAATTG+GGG | 0.567079 | 3:-95807358 | MsG0380017398.01.T01:CDS |
| GATTAAGGCTTTGATAGCAA+TGG | 0.569015 | 3:-95807715 | MsG0380017398.01.T01:CDS |
| CAAGGTTTATAATGATTACA+CGG | 0.569226 | 3:+95807994 | None:intergenic |
| CTTTGAAGAGAAAAGTGAAA+GGG | 0.585350 | 3:+95808258 | None:intergenic |
| AGAATCAGCTATGGCTATAG+CGG | 0.587380 | 3:-95807793 | MsG0380017398.01.T01:CDS |
| ATACAAGTGGAAATTGGGGA+AGG | 0.606082 | 3:-95807354 | MsG0380017398.01.T01:CDS |
| CTCTAAAAGAGCTTCAATGG+CGG | 0.608003 | 3:+95808223 | None:intergenic |
| GATACTGAGATTGGTGAAGG+AGG | 0.619602 | 3:-95807295 | MsG0380017398.01.T01:CDS |
| GCTTTGAAGAGAAAAGTGAA+AGG | 0.623593 | 3:+95808257 | None:intergenic |
| GGTTCGAGAAGAATCAGCTA+TGG | 0.629018 | 3:-95807802 | MsG0380017398.01.T01:CDS |
| GATTAGAGAAAAGAGGTTAG+GGG | 0.631517 | 3:-95807424 | MsG0380017398.01.T01:CDS |
| GTTAGTGCTGAGATATTGTG+GGG | 0.668901 | 3:-95807243 | MsG0380017398.01.T01:CDS |
| GGTGCTTAGAAGGTTCATCA+GGG | 0.684594 | 3:+95808342 | None:intergenic |
| AGTTGAGAAGAGTAAGAACA+CGG | 0.702245 | 3:+95808321 | None:intergenic |
| CTATTGTTGAGTTTGAACAG+AGG | 0.712522 | 3:-95807926 | MsG0380017398.01.T01:CDS |
| ACTGCAAGAACAGAACAACG+AGG | 0.856159 | 3:-95807964 | MsG0380017398.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATCTTGTATGAAATGATT+TGG | + | Chr3:95807517-95807536 | None:intergenic | 20.0% |
| !! | AACAATAAACCAATAATCTT+AGG | + | Chr3:95807977-95807996 | None:intergenic | 20.0% |
| !! | GATAAAATTTCATCAACTAA+AGG | + | Chr3:95807941-95807960 | None:intergenic | 20.0% |
| !!! | GTTGATGAAATTTTATCTAA+TGG | - | Chr3:95807944-95807963 | MsG0380017398.01.T01:CDS | 20.0% |
| !!! | GTTTTTGAACTTTGTTATAT+TGG | - | Chr3:95808037-95808056 | MsG0380017398.01.T01:CDS | 20.0% |
| !!! | TCAACAAAAATGTTTTTGTT+GGG | - | Chr3:95807819-95807838 | MsG0380017398.01.T01:CDS | 20.0% |
| !!! | TTCAACAAAAATGTTTTTGT+TGG | - | Chr3:95807818-95807837 | MsG0380017398.01.T01:CDS | 20.0% |
| ! | ATTAGATGAGAAGATAGTTA+TGG | + | Chr3:95807393-95807412 | None:intergenic | 25.0% |
| ! | CAAGGTTTATAATGATTACA+CGG | + | Chr3:95807586-95807605 | None:intergenic | 25.0% |
| ! | GAAAACTTGGAAAAATGTAA+AGG | - | Chr3:95807464-95807483 | MsG0380017398.01.T01:CDS | 25.0% |
| ! | GTCTAAATAAGTCTGAATTT+CGG | + | Chr3:95807564-95807583 | None:intergenic | 25.0% |
| ! | TTGGAAAAATGTAAAGGTTA+TGG | - | Chr3:95807470-95807489 | MsG0380017398.01.T01:CDS | 25.0% |
| AAAAGAGGATTTATGTGATG+TGG | - | Chr3:95808126-95808145 | MsG0380017398.01.T01:CDS | 30.0% | |
| AGAAAAAAGAGAGGTGAAAT+TGG | - | Chr3:95808258-95808277 | MsG0380017398.01.T01:CDS | 30.0% | |
| AGATTAGAGAAAAGAGGTTA+GGG | - | Chr3:95808152-95808171 | MsG0380017398.01.T01:CDS | 30.0% | |
| GGAATGATTCCTAAGATTAT+TGG | - | Chr3:95807965-95807984 | MsG0380017398.01.T01:CDS | 30.0% | |
| TAAAACCTTGATCGAAAACT+TGG | - | Chr3:95807451-95807470 | MsG0380017398.01.T01:CDS | 30.0% | |
| TGAGAGAGATTGTAAAGATT+TGG | + | Chr3:95807416-95807435 | None:intergenic | 30.0% | |
| TTGCAATACAAGTGGAAATT+GGG | - | Chr3:95808218-95808237 | MsG0380017398.01.T01:CDS | 30.0% | |
| TTTGCAATACAAGTGGAAAT+TGG | - | Chr3:95808217-95808236 | MsG0380017398.01.T01:CDS | 30.0% | |
| ! | AGTTTTCGATCAAGGTTTTA+AGG | + | Chr3:95807451-95807470 | None:intergenic | 30.0% |
| ! | CTTTGAAGAGAAAAGTGAAA+GGG | + | Chr3:95807322-95807341 | None:intergenic | 30.0% |
| ! | GATGATTTTGCAAAGAAAAG+AGG | - | Chr3:95808111-95808130 | MsG0380017398.01.T01:CDS | 30.0% |
| ! | TTTTTCCAAGTTTTCGATCA+AGG | + | Chr3:95807459-95807478 | None:intergenic | 30.0% |
| !! | AACCAACATACGTTCTAAAA+GGG | + | Chr3:95807735-95807754 | None:intergenic | 30.0% |
| !! | GTTTTCGATCAAGGTTTTAA+GGG | + | Chr3:95807450-95807469 | None:intergenic | 30.0% |
| !!! | GGTTTTGATTTGAAGATCTT+TGG | + | Chr3:95807297-95807316 | None:intergenic | 30.0% |
| !!! | TTTGTTGGGTTAGTTTTGAT+GGG | - | Chr3:95807833-95807852 | MsG0380017398.01.T01:CDS | 30.0% |
| !!! | TTTTGTTGGGTTAGTTTTGA+TGG | - | Chr3:95807832-95807851 | MsG0380017398.01.T01:CDS | 30.0% |
| AGTCTGAATTTCGGATTCTA+AGG | + | Chr3:95807555-95807574 | None:intergenic | 35.0% | |
| AGTTGAGAAGAGTAAGAACA+CGG | + | Chr3:95807259-95807278 | None:intergenic | 35.0% | |
| CTATTGTTGAGTTTGAACAG+AGG | - | Chr3:95807651-95807670 | MsG0380017398.01.T01:CDS | 35.0% | |
| GAAATTGGAGATACTGAGAT+TGG | - | Chr3:95808273-95808292 | MsG0380017398.01.T01:CDS | 35.0% | |
| GAGATTAGAGAAAAGAGGTT+AGG | - | Chr3:95808151-95808170 | MsG0380017398.01.T01:CDS | 35.0% | |
| GAGTAGTGAAGAAAAAAGAG+AGG | - | Chr3:95808249-95808268 | MsG0380017398.01.T01:CDS | 35.0% | |
| GATTAGAGAAAAGAGGTTAG+GGG | - | Chr3:95808153-95808172 | MsG0380017398.01.T01:CDS | 35.0% | |
| GTTTGAACAGAGGTTATTGT+CGG | - | Chr3:95807661-95807680 | MsG0380017398.01.T01:CDS | 35.0% | |
| TGAACCCTTTACTTCGAAAA+GGG | - | Chr3:95807754-95807773 | MsG0380017398.01.T01:CDS | 35.0% | |
| TGCAATACAAGTGGAAATTG+GGG | - | Chr3:95808219-95808238 | MsG0380017398.01.T01:CDS | 35.0% | |
| TGGAAGAGATTAGAGAAAAG+AGG | - | Chr3:95808146-95808165 | MsG0380017398.01.T01:CDS | 35.0% | |
| TTCGAGATTTGCAATACAAG+TGG | - | Chr3:95808210-95808229 | MsG0380017398.01.T01:CDS | 35.0% | |
| TTGAACCCTTTACTTCGAAA+AGG | - | Chr3:95807753-95807772 | MsG0380017398.01.T01:CDS | 35.0% | |
| TTTGAACAGAGGTTATTGTC+GGG | - | Chr3:95807662-95807681 | MsG0380017398.01.T01:CDS | 35.0% | |
| ! | ATCTTTGGAAGCTTGTTTGA+GGG | + | Chr3:95807282-95807301 | None:intergenic | 35.0% |
| ! | ATGAAATGATTTGGCGTTGA+AGG | + | Chr3:95807508-95807527 | None:intergenic | 35.0% |
| ! | CAACCAACATACGTTCTAAA+AGG | + | Chr3:95807736-95807755 | None:intergenic | 35.0% |
| ! | GATTAAGGCTTTGATAGCAA+TGG | - | Chr3:95807862-95807881 | MsG0380017398.01.T01:CDS | 35.0% |
| ! | TGTTAGTGCTGAGATATTGT+GGG | - | Chr3:95808333-95808352 | MsG0380017398.01.T01:CDS | 35.0% |
| !! | AAGCTCTAAAAGAGCTTCAA+TGG | + | Chr3:95807360-95807379 | None:intergenic | 35.0% |
| !! | GCTTTGAAGAGAAAAGTGAA+AGG | + | Chr3:95807323-95807342 | None:intergenic | 35.0% |
| !!! | GTTATTGTCGGGTTTTGATT+TGG | - | Chr3:95807673-95807692 | MsG0380017398.01.T01:CDS | 35.0% |
| !!! | TACCCTTTTAGAACGTATGT+TGG | - | Chr3:95807730-95807749 | MsG0380017398.01.T01:CDS | 35.0% |
| AGAATCAGCTATGGCTATAG+CGG | - | Chr3:95807784-95807803 | MsG0380017398.01.T01:CDS | 40.0% | |
| AGCAATGTTACAGCAAGAGT+TGG | - | Chr3:95808078-95808097 | MsG0380017398.01.T01:CDS | 40.0% | |
| ATACAAGTGGAAATTGGGGA+AGG | - | Chr3:95808223-95808242 | MsG0380017398.01.T01:CDS | 40.0% | |
| GAAGAGAAAAGTGAAAGGGT+TGG | + | Chr3:95807318-95807337 | None:intergenic | 40.0% | |
| GCTATCAAAGCCTTAATCGT+TGG | + | Chr3:95807860-95807879 | None:intergenic | 40.0% | |
| ! | AATGATTTGGCGTTGAAGGA+TGG | + | Chr3:95807504-95807523 | None:intergenic | 40.0% |
| ! | CGAACCCTTTTCGAAGTAAA+GGG | + | Chr3:95807761-95807780 | None:intergenic | 40.0% |
| ! | CTCTAAAAGAGCTTCAATGG+CGG | + | Chr3:95807357-95807376 | None:intergenic | 40.0% |
| ! | CTGTTAGTGCTGAGATATTG+TGG | - | Chr3:95808332-95808351 | MsG0380017398.01.T01:CDS | 40.0% |
| ! | GATCTTTGGAAGCTTGTTTG+AGG | + | Chr3:95807283-95807302 | None:intergenic | 40.0% |
| ! | GTTAGTGCTGAGATATTGTG+GGG | - | Chr3:95808334-95808353 | MsG0380017398.01.T01:CDS | 40.0% |
| ! | TCGAACCCTTTTCGAAGTAA+AGG | + | Chr3:95807762-95807781 | None:intergenic | 40.0% |
| ! | TGATTTGTGTCTTCGTGTTG+CGG | - | Chr3:95808003-95808022 | MsG0380017398.01.T01:CDS | 40.0% |
| ! | TTCAATGGCGGCTTTTGAAT+CGG | + | Chr3:95807345-95807364 | None:intergenic | 40.0% |
| ! | TTTGATGGGTCCAACGATTA+AGG | - | Chr3:95807847-95807866 | MsG0380017398.01.T01:CDS | 40.0% |
| ACAGAACAACGAGGATGAAC+AGG | - | Chr3:95807622-95807641 | MsG0380017398.01.T01:CDS | 45.0% | |
| ACTGCAAGAACAGAACAACG+AGG | - | Chr3:95807613-95807632 | MsG0380017398.01.T01:CDS | 45.0% | |
| CAACGAGGATGAACAGGTTA+AGG | - | Chr3:95807628-95807647 | MsG0380017398.01.T01:CDS | 45.0% | |
| GATACTGAGATTGGTGAAGG+AGG | - | Chr3:95808282-95808301 | MsG0380017398.01.T01:CDS | 45.0% | |
| GGAGATACTGAGATTGGTGA+AGG | - | Chr3:95808279-95808298 | MsG0380017398.01.T01:CDS | 45.0% | |
| GGTGCTTAGAAGGTTCATCA+GGG | + | Chr3:95807238-95807257 | None:intergenic | 45.0% | |
| GGTTCGAGAAGAATCAGCTA+TGG | - | Chr3:95807775-95807794 | MsG0380017398.01.T01:CDS | 45.0% | |
| GTAAGAACACGGTGCTTAGA+AGG | + | Chr3:95807248-95807267 | None:intergenic | 45.0% | |
| GTTCTTGCAGTGTCTTCACA+AGG | + | Chr3:95807604-95807623 | None:intergenic | 45.0% | |
| TAGAGAAAAGAGGTTAGGGG+TGG | - | Chr3:95808156-95808175 | MsG0380017398.01.T01:CDS | 45.0% | |
| ! | GGCTTCGGAGTGTTCTATAA+AGG | - | Chr3:95807883-95807902 | MsG0380017398.01.T01:CDS | 45.0% |
| ! | GGCTTTGATAGCAATGGCTT+CGG | - | Chr3:95807868-95807887 | MsG0380017398.01.T01:CDS | 45.0% |
| !! | AGCTATGGCTATAGCGGATT+TGG | - | Chr3:95807790-95807809 | MsG0380017398.01.T01:CDS | 45.0% |
| CGGTGCTTAGAAGGTTCATC+AGG | + | Chr3:95807239-95807258 | None:intergenic | 50.0% | |
| GTGTCTTCGTGTTGCGGCTT+TGG | - | Chr3:95808009-95808028 | MsG0380017398.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 95807230 | 95808369 | 95807230 | ID=MsG0380017398.01;Name=MsG0380017398.01 |
| Chr3 | mRNA | 95807230 | 95808369 | 95807230 | ID=MsG0380017398.01.T01;Parent=MsG0380017398.01;Name=MsG0380017398.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|379 |
| Chr3 | exon | 95807230 | 95808369 | 95807230 | ID=MsG0380017398.01.T01:exon:4839;Parent=MsG0380017398.01.T01 |
| Chr3 | CDS | 95807230 | 95808369 | 95807230 | ID=MsG0380017398.01.T01:cds;Parent=MsG0380017398.01.T01 |
| Gene Sequence |
| Protein sequence |
