AlfalfaGEDB | MsG0180000595.01

M. sativa cultivar ZhongmuNo.1 / MsG0180000595.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000595.01.T01	AES59029.1	93.151	146	10	0	1	146	159	304	1.56E-78	257

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000595.01.T01	A0A396JFC3	93.151	146	10	0	1	146	159	304	1.08e-78	257

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000594.01	MsG0180000595.01	0.919986	2.264566e-87	7.487181e-83
MsG0180000595.01	MsG0180000596.01	0.925123	2.808035e-90	1.231361e-85
MsG0180000595.01	MsG0380015812.01	0.802418	5.662794e-49	2.772655e-46
MsG0180000595.01	MsG0380017398.01	0.817714	2.856910e-52	2.096366e-49
MsG0180000595.01	MsG0380017798.01	-0.819098	1.388133e-52	1.058196e-49
MsG0180000595.01	MsG0680032814.01	0.833865	4.171398e-56	4.866174e-53

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000595.01.T01	MTR_1g012620	93.151	146	10	0	1	146	159	304	1.88e-82	257

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000595.01.T01	AT4G32190	47.518	141	74	0	1	141	170	310	4.06e-26	103

Find 33 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAAGGTTGCAGAGGCCTCTT+TGG	0.261894	1:+8439449	MsG0180000595.01.T01:CDS
GAAGGAGGCGACGGCTAGTT+TGG	0.320741	1:+8439113	MsG0180000595.01.T01:CDS
AGGAAATGAAGCTCCGGCTT+AGG	0.372074	1:+8439157	MsG0180000595.01.T01:CDS
AAATTGAGCTTCTAGAATTA+CGG	0.387513	1:+8439355	MsG0180000595.01.T01:CDS
GGAAATGAAGCTCCGGCTTA+GGG	0.388122	1:+8439158	MsG0180000595.01.T01:CDS
ATAGTGAGATTGATGGTTTA+CGG	0.396914	1:+8439187	MsG0180000595.01.T01:CDS
GAGATGGAGAAAATGAAGAT+TGG	0.405764	1:+8439234	MsG0180000595.01.T01:CDS
GACTGGAAGAGGAGGCTTCA+AGG	0.407228	1:+8439517	MsG0180000595.01.T01:CDS
TAGTGAGATTGATGGTTTAC+GGG	0.413981	1:+8439188	MsG0180000595.01.T01:CDS
GGAGAAAATGAAGATTGGTT+TGG	0.423670	1:+8439239	MsG0180000595.01.T01:CDS
GCTAGTTTGGTTTCTCAAGC+TGG	0.442794	1:+8439126	MsG0180000595.01.T01:CDS
ACGGGATGCGCTGTCTTTGA+AGG	0.447564	1:+8439206	MsG0180000595.01.T01:CDS
GATCGTGATAGTGAGATTGA+TGG	0.456862	1:+8439180	MsG0180000595.01.T01:CDS
TGATTCTGAGCTTAGACAAA+AGG	0.457472	1:+8439290	MsG0180000595.01.T01:CDS
GGAGGCTTCAAGGCATGCAC+AGG	0.466912	1:+8439527	MsG0180000595.01.T01:CDS
TTCTCAAGCTGGACAGATAG+AGG	0.486214	1:+8439137	MsG0180000595.01.T01:CDS
CACTATCACGATCCCTAAGC+CGG	0.519235	1:-8439170	None:intergenic
ACAAGAGGAACTCAAGAGAC+TGG	0.522581	1:+8439500	MsG0180000595.01.T01:CDS
ACGTCTATCCGTGGCTGCGA+GGG	0.526538	1:+8439407	MsG0180000595.01.T01:CDS
GTCTTTGAAGGAAGAAGAGA+TGG	0.530883	1:+8439218	MsG0180000595.01.T01:CDS
TTCAACATCCCTCGCAGCCA+CGG	0.543338	1:-8439415	None:intergenic
AGATAGAGGAAATGAAGCTC+CGG	0.558398	1:+8439151	MsG0180000595.01.T01:CDS
GTGGCTGCGAGGGATGTTGA+AGG	0.560868	1:+8439417	MsG0180000595.01.T01:CDS
GGAACTCAAGAGACTGGAAG+AGG	0.572397	1:+8439506	MsG0180000595.01.T01:CDS
TACGTCTATCCGTGGCTGCG+AGG	0.593135	1:+8439406	MsG0180000595.01.T01:CDS
ACTCAAGAGACTGGAAGAGG+AGG	0.605945	1:+8439509	MsG0180000595.01.T01:CDS
TGGAAAAGCACACGATGGAG+TGG	0.609324	1:+8439469	MsG0180000595.01.T01:CDS
GGAGTGGTTGTTAACACAAG+AGG	0.610855	1:+8439485	MsG0180000595.01.T01:CDS
AGATGAGATGAAGGAGGCGA+CGG	0.624465	1:+8439104	None:intergenic
GAGGCTTCAAGGCATGCACA+GGG	0.643450	1:+8439528	MsG0180000595.01.T01:CDS
AGAAGAACTACGTCTATCCG+TGG	0.663326	1:+8439398	MsG0180000595.01.T01:CDS
GCTTGAAGATGAGATGAAGG+AGG	0.679304	1:+8439098	None:intergenic
CTCTTTGGAAAAGCACACGA+TGG	0.698049	1:+8439464	MsG0180000595.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AAATTGAGCTTCTAGAATTA+CGG	+	Chr1:8439355-8439374	MsG0180000595.01.T01:CDS	25.0%
!	ATAGTGAGATTGATGGTTTA+CGG	+	Chr1:8439187-8439206	MsG0180000595.01.T01:CDS	30.0%
	GAGATGGAGAAAATGAAGAT+TGG	+	Chr1:8439234-8439253	MsG0180000595.01.T01:CDS	35.0%
	TGATTCTGAGCTTAGACAAA+AGG	+	Chr1:8439290-8439309	MsG0180000595.01.T01:CDS	35.0%
	TGTTGAAGGAGAAAAACTCA+AGG	+	Chr1:8439431-8439450	MsG0180000595.01.T01:CDS	35.0%
!	GGAGAAAATGAAGATTGGTT+TGG	+	Chr1:8439239-8439258	MsG0180000595.01.T01:CDS	35.0%
!	TAGTGAGATTGATGGTTTAC+GGG	+	Chr1:8439188-8439207	MsG0180000595.01.T01:CDS	35.0%
	AGAAAAACTCAAGGTTGCAG+AGG	+	Chr1:8439440-8439459	MsG0180000595.01.T01:CDS	40.0%
	AGATAGAGGAAATGAAGCTC+CGG	+	Chr1:8439151-8439170	MsG0180000595.01.T01:CDS	40.0%
!	GATCGTGATAGTGAGATTGA+TGG	+	Chr1:8439180-8439199	MsG0180000595.01.T01:CDS	40.0%
!	GTCTTTGAAGGAAGAAGAGA+TGG	+	Chr1:8439218-8439237	MsG0180000595.01.T01:CDS	40.0%
	ACAAGAGGAACTCAAGAGAC+TGG	+	Chr1:8439500-8439519	MsG0180000595.01.T01:CDS	45.0%
	AGAAGAACTACGTCTATCCG+TGG	+	Chr1:8439398-8439417	MsG0180000595.01.T01:CDS	45.0%
	GGAGTGGTTGTTAACACAAG+AGG	+	Chr1:8439485-8439504	MsG0180000595.01.T01:CDS	45.0%
	TTCTCAAGCTGGACAGATAG+AGG	+	Chr1:8439137-8439156	MsG0180000595.01.T01:CDS	45.0%
!	CATCGTGTGCTTTTCCAAAG+AGG	-	Chr1:8439466-8439485	None:intergenic	45.0%
!	CTCTTTGGAAAAGCACACGA+TGG	+	Chr1:8439464-8439483	MsG0180000595.01.T01:CDS	45.0%
!	GCTAGTTTGGTTTCTCAAGC+TGG	+	Chr1:8439126-8439145	MsG0180000595.01.T01:CDS	45.0%
	ACTCAAGAGACTGGAAGAGG+AGG	+	Chr1:8439509-8439528	MsG0180000595.01.T01:CDS	50.0%
	AGGAAATGAAGCTCCGGCTT+AGG	+	Chr1:8439157-8439176	MsG0180000595.01.T01:CDS	50.0%
	CACTATCACGATCCCTAAGC+CGG	-	Chr1:8439173-8439192	None:intergenic	50.0%
	GGAAATGAAGCTCCGGCTTA+GGG	+	Chr1:8439158-8439177	MsG0180000595.01.T01:CDS	50.0%
	GGAACTCAAGAGACTGGAAG+AGG	+	Chr1:8439506-8439525	MsG0180000595.01.T01:CDS	50.0%
!	TGGAAAAGCACACGATGGAG+TGG	+	Chr1:8439469-8439488	MsG0180000595.01.T01:CDS	50.0%
	ACGGGATGCGCTGTCTTTGA+AGG	+	Chr1:8439206-8439225	MsG0180000595.01.T01:CDS	55.0%
	CAAGGTTGCAGAGGCCTCTT+TGG	+	Chr1:8439449-8439468	MsG0180000595.01.T01:CDS	55.0%
	GACTGGAAGAGGAGGCTTCA+AGG	+	Chr1:8439517-8439536	MsG0180000595.01.T01:CDS	55.0%
	GAGGCTTCAAGGCATGCACA+GGG	+	Chr1:8439528-8439547	MsG0180000595.01.T01:CDS	55.0%
	TTCAACATCCCTCGCAGCCA+CGG	-	Chr1:8439418-8439437	None:intergenic	55.0%
	ACGTCTATCCGTGGCTGCGA+GGG	+	Chr1:8439407-8439426	MsG0180000595.01.T01:CDS	60.0%
	GGAGGCTTCAAGGCATGCAC+AGG	+	Chr1:8439527-8439546	MsG0180000595.01.T01:CDS	60.0%
	GTGGCTGCGAGGGATGTTGA+AGG	+	Chr1:8439417-8439436	MsG0180000595.01.T01:CDS	60.0%
	TACGTCTATCCGTGGCTGCG+AGG	+	Chr1:8439406-8439425	MsG0180000595.01.T01:CDS	60.0%
!	GAAGGAGGCGACGGCTAGTT+TGG	+	Chr1:8439113-8439132	MsG0180000595.01.T01:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	8439111	8439566	8439111	ID=MsG0180000595.01;Name=MsG0180000595.01
Chr1	mRNA	8439111	8439566	8439111	ID=MsG0180000595.01.T01;Parent=MsG0180000595.01;Name=MsG0180000595.01.T01;_AED=0.49;_eAED=0.49;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|151
Chr1	exon	8439111	8439566	8439111	ID=MsG0180000595.01.T01:exon:2471;Parent=MsG0180000595.01.T01
Chr1	CDS	8439111	8439566	8439111	ID=MsG0180000595.01.T01:cds;Parent=MsG0180000595.01.T01

Gene Sequence

>MsG0180000595.01.T01

ATGAAGGAGGCGACGGCTAGTTTGGTTTCTCAAGCTGGACAGATAGAGGAAATGAAGCTCCGGCTTAGGGATCGTGATAGTGAGATTGATGGTTTACGGGATGCGCTGTCTTTGAAGGAAGAAGAGATGGAGAAAATGAAGATTGGTTTGGCTAAGAAGAGTGAAGAAGCTGCTTATGTTGATTCTGAGCTTAGACAAAAGGTTCAGCTTCTGAGTGAAGCAAATGAGATCGTGAAGAAACAAGAAATTGAGCTTCTAGAATTACGGAGTGTTGTACGACAAAGAGAAGAAGAACTACGTCTATCCGTGGCTGCGAGGGATGTTGAAGGAGAAAAACTCAAGGTTGCAGAGGCCTCTTTGGAAAAGCACACGATGGAGTGGTTGTTAACACAAGAGGAACTCAAGAGACTGGAAGAGGAGGCTTCAAGGCATGCACAGGGAGTAGTGAAACGCTAG

Protein sequence

>MsG0180000595.01.T01

MKEATASLVSQAGQIEEMKLRLRDRDSEIDGLRDALSLKEEEMEKMKIGLAKKSEEAAYVDSELRQKVQLLSEANEIVKKQEIELLELRSVVRQREEELRLSVAARDVEGEKLKVAEASLEKHTMEWLLTQEELKRLEEEASRHAQGVVKR*