Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017546.01.T01 | XP_003603580.1 | 90.857 | 175 | 12 | 1 | 1 | 175 | 125 | 295 | 5.17E-112 | 330 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017546.01.T01 | Q9FUD3 | 46.591 | 176 | 74 | 7 | 1 | 175 | 121 | 277 | 2.66E-35 | 127 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017546.01.T01 | G7J2W2 | 90.857 | 175 | 12 | 1 | 1 | 175 | 125 | 295 | 2.47e-112 | 330 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380014573.01 | MsG0380017546.01 | 0.804353 | 2.249841e-49 | 1.157511e-46 |
| MsG0380017546.01 | MsG0780038386.01 | 0.822122 | 2.804882e-53 | 2.326528e-50 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017546.01.T01 | MTR_3g109310 | 90.857 | 175 | 12 | 1 | 1 | 175 | 125 | 295 | 6.27e-116 | 330 |
| MsG0380017546.01.T01 | MTR_3g110010 | 82.540 | 126 | 22 | 0 | 6 | 131 | 633 | 758 | 1.43e-59 | 197 |
| MsG0380017546.01.T01 | MTR_3g109982 | 81.746 | 126 | 23 | 0 | 6 | 131 | 649 | 774 | 4.97e-59 | 196 |
| MsG0380017546.01.T01 | MTR_1g008990 | 61.017 | 177 | 60 | 7 | 1 | 175 | 170 | 339 | 4.40e-53 | 172 |
| MsG0380017546.01.T01 | MTR_1g008990 | 73.684 | 76 | 20 | 0 | 1 | 76 | 170 | 245 | 8.58e-24 | 94.4 |
| MsG0380017546.01.T01 | MTR_1g080920 | 52.778 | 108 | 47 | 2 | 1 | 108 | 209 | 312 | 1.73e-19 | 84.7 |
| MsG0380017546.01.T01 | MTR_7g115120 | 58.537 | 82 | 34 | 0 | 2 | 83 | 195 | 276 | 4.17e-18 | 80.9 |
| MsG0380017546.01.T01 | MTR_7g115140 | 47.945 | 73 | 38 | 0 | 2 | 74 | 134 | 206 | 8.50e-14 | 67.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017546.01.T01 | AT5G24800 | 46.591 | 176 | 74 | 7 | 1 | 175 | 121 | 277 | 2.71e-36 | 127 |
| MsG0380017546.01.T01 | AT5G28770 | 53.465 | 101 | 43 | 2 | 1 | 101 | 152 | 248 | 7.25e-19 | 82.0 |
| MsG0380017546.01.T01 | AT5G28770 | 53.465 | 101 | 43 | 2 | 1 | 101 | 145 | 241 | 7.59e-19 | 82.0 |
| MsG0380017546.01.T01 | AT5G28770 | 58.667 | 75 | 31 | 0 | 1 | 75 | 152 | 226 | 2.63e-15 | 71.6 |
| MsG0380017546.01.T01 | AT4G02640 | 46.316 | 95 | 48 | 1 | 1 | 95 | 222 | 313 | 1.68e-12 | 65.1 |
| MsG0380017546.01.T01 | AT4G02640 | 49.398 | 83 | 42 | 0 | 1 | 83 | 222 | 304 | 1.69e-12 | 65.1 |
| MsG0380017546.01.T01 | AT4G02640 | 49.398 | 83 | 42 | 0 | 1 | 83 | 216 | 298 | 1.91e-12 | 64.7 |
| MsG0380017546.01.T01 | AT4G02640 | 49.398 | 83 | 42 | 0 | 1 | 83 | 216 | 298 | 1.97e-12 | 64.7 |
Find 37 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTATGAGTTGTGTAACTATT+TGG | 0.238588 | 3:+97735651 | MsG0380017546.01.T01:CDS |
| AATTCAAATCTTGCTGGGTT+TGG | 0.297271 | 3:+97735575 | MsG0380017546.01.T01:CDS |
| TCTTGCTGGGTTTGGAAACT+TGG | 0.301540 | 3:+97735583 | MsG0380017546.01.T01:CDS |
| AACACTACACAAACAGTTTA+CGG | 0.338361 | 3:+97735184 | MsG0380017546.01.T01:CDS |
| GTGTAACTATTTGGCCTTAA+GGG | 0.360767 | 3:+97735660 | MsG0380017546.01.T01:CDS |
| TGCCTCATACGGTGGTGTTC+CGG | 0.376655 | 3:+97735541 | MsG0380017546.01.T01:CDS |
| GTGCACAATTCAAATCTTGC+TGG | 0.391606 | 3:+97735569 | MsG0380017546.01.T01:CDS |
| ATTTCCCTGCTGGACATTGA+TGG | 0.397970 | 3:-97735516 | None:intergenic |
| TGATGTGGTGACAGAGCTTC+TGG | 0.398710 | 3:-97735396 | None:intergenic |
| TGTGTAACTATTTGGCCTTA+AGG | 0.401972 | 3:+97735659 | MsG0380017546.01.T01:CDS |
| TTTGAATTGTGCACGCCAAC+CGG | 0.414397 | 3:-97735560 | None:intergenic |
| GGTGAAGTTAGCTGAGGATA+TGG | 0.427027 | 3:+97735370 | MsG0380017546.01.T01:intron |
| AACCGGAACACCACCGTATG+AGG | 0.428469 | 3:-97735543 | None:intergenic |
| TTTGTCTGAACTTGAGTCTC+AGG | 0.431765 | 3:+97734858 | MsG0380017546.01.T01:CDS |
| CCCTGCTGGACATTGATGGT+TGG | 0.492984 | 3:-97735512 | None:intergenic |
| TGCACAATTCAAATCTTGCT+GGG | 0.493012 | 3:+97735570 | MsG0380017546.01.T01:CDS |
| ATGAGGCACTATTTCCCTGC+TGG | 0.505822 | 3:-97735526 | None:intergenic |
| GCCAACCATCAATGTCCAGC+AGG | 0.512549 | 3:+97735511 | MsG0380017546.01.T01:CDS |
| CACAACTCATAGCATTGCTT+AGG | 0.535820 | 3:-97735640 | None:intergenic |
| TGTAGAAGCTTTGAGAGCTA+AGG | 0.540256 | 3:+97735262 | MsG0380017546.01.T01:CDS |
| TACAATGATGTCGTCGACAA+CGG | 0.542670 | 3:+97735614 | MsG0380017546.01.T01:CDS |
| TTTGACAGAAATACCTTCTG+AGG | 0.545576 | 3:-97734702 | None:intergenic |
| AAACTGATTGTTTAGTGATG+TGG | 0.571542 | 3:-97735411 | None:intergenic |
| CAGGGAAATAGTGCCTCATA+CGG | 0.576934 | 3:+97735530 | MsG0380017546.01.T01:CDS |
| TCATACGGTGGTGTTCCGGT+TGG | 0.585749 | 3:+97735545 | MsG0380017546.01.T01:CDS |
| TTGATATGAAACGCCTCAGA+AGG | 0.589092 | 3:+97734689 | None:intergenic |
| TACTATTCAACAATTTCATG+AGG | 0.600013 | 3:+97735208 | MsG0380017546.01.T01:CDS |
| GCGAGTCAACCAAGAGGTCA+AGG | 0.603161 | 3:+97734818 | MsG0380017546.01.T01:CDS |
| GGAAATAGTGCCTCATACGG+TGG | 0.626744 | 3:+97735533 | MsG0380017546.01.T01:CDS |
| TTAGTAGTCATGTTGAGTTG+TGG | 0.648094 | 3:-97735464 | None:intergenic |
| CCAACCATCAATGTCCAGCA+GGG | 0.666005 | 3:+97735512 | MsG0380017546.01.T01:CDS |
| ATAATAGGTGAAGTTAGCTG+AGG | 0.671237 | 3:+97735364 | MsG0380017546.01.T01:intron |
| GTAGTCATGTTGAGTTGTGG+CGG | 0.676620 | 3:-97735461 | None:intergenic |
| CAAATTGCGAGTCAACCAAG+AGG | 0.697375 | 3:+97734812 | MsG0380017546.01.T01:CDS |
| TTGGCGAAACATGTGGCATG+CGG | 0.727060 | 3:-97735493 | None:intergenic |
| AGTCAACCAAGAGGTCAAGG+TGG | 0.727607 | 3:+97734821 | MsG0380017546.01.T01:CDS |
| TTGATGGTTGGCGAAACATG+TGG | 0.736785 | 3:-97735500 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAACATATACTATTATAAT+AGG | + | Chr3:97735349-97735368 | MsG0380017546.01.T01:intron | 10.0% |
| !! | ACAACACTTCTAATTATATA+TGG | + | Chr3:97734741-97734760 | MsG0380017546.01.T01:intron | 20.0% |
| ! | TACTATTCAACAATTTCATG+AGG | + | Chr3:97735208-97735227 | MsG0380017546.01.T01:CDS | 25.0% |
| ! | TGAGAGATCAAAATTCAAAT+GGG | - | Chr3:97735137-97735156 | None:intergenic | 25.0% |
| !!! | AATTTTGTTTCTGGAATTGT+AGG | + | Chr3:97734908-97734927 | MsG0380017546.01.T01:intron | 25.0% |
| !!! | GAAGTCATTAATTTTGTTTC+TGG | + | Chr3:97734899-97734918 | MsG0380017546.01.T01:intron | 25.0% |
| AAACTGATTGTTTAGTGATG+TGG | - | Chr3:97735414-97735433 | None:intergenic | 30.0% | |
| AACACTACACAAACAGTTTA+CGG | + | Chr3:97735184-97735203 | MsG0380017546.01.T01:CDS | 30.0% | |
| CTATGAGTTGTGTAACTATT+TGG | + | Chr3:97735651-97735670 | MsG0380017546.01.T01:CDS | 30.0% | |
| CTGAGAGATCAAAATTCAAA+TGG | - | Chr3:97735138-97735157 | None:intergenic | 30.0% | |
| GTCACTGAAATAGAAATAGT+AGG | - | Chr3:97735005-97735024 | None:intergenic | 30.0% | |
| TGATATAATGATTCGTGACA+TGG | + | Chr3:97734775-97734794 | MsG0380017546.01.T01:intron | 30.0% | |
| ! | TAATTCTGAGAGCAAATTCT+AGG | + | Chr3:97734976-97734995 | MsG0380017546.01.T01:intron | 30.0% |
| !! | CATTTGAATTTTGATCTCTC+AGG | + | Chr3:97735136-97735155 | MsG0380017546.01.T01:intron | 30.0% |
| AATTCAAATCTTGCTGGGTT+TGG | + | Chr3:97735575-97735594 | MsG0380017546.01.T01:CDS | 35.0% | |
| ATAATAGGTGAAGTTAGCTG+AGG | + | Chr3:97735364-97735383 | MsG0380017546.01.T01:intron | 35.0% | |
| CACAATTTGATATCTCATGC+AGG | + | Chr3:97734938-97734957 | MsG0380017546.01.T01:intron | 35.0% | |
| TGCACAATTCAAATCTTGCT+GGG | + | Chr3:97735570-97735589 | MsG0380017546.01.T01:CDS | 35.0% | |
| TTTGACAGAAATACCTTCTG+AGG | - | Chr3:97734705-97734724 | None:intergenic | 35.0% | |
| ! | CAGGTAACTAATCAGTGTTT+TGG | + | Chr3:97734877-97734896 | MsG0380017546.01.T01:intron | 35.0% |
| ! | TTAGTAGTCATGTTGAGTTG+TGG | - | Chr3:97735467-97735486 | None:intergenic | 35.0% |
| !! | CTACATCTGATTTTAGCACA+CGG | - | Chr3:97735247-97735266 | None:intergenic | 35.0% |
| !! | TTGTTTCTGGAATTGTAGGT+AGG | + | Chr3:97734912-97734931 | MsG0380017546.01.T01:intron | 35.0% |
| ATTCGTGACATGGTTTCAGA+AGG | + | Chr3:97734785-97734804 | MsG0380017546.01.T01:intron | 40.0% | |
| CACAACTCATAGCATTGCTT+AGG | - | Chr3:97735643-97735662 | None:intergenic | 40.0% | |
| GTGCACAATTCAAATCTTGC+TGG | + | Chr3:97735569-97735588 | MsG0380017546.01.T01:CDS | 40.0% | |
| TACAATGATGTCGTCGACAA+CGG | + | Chr3:97735614-97735633 | MsG0380017546.01.T01:CDS | 40.0% | |
| TTCGTGACATGGTTTCAGAA+GGG | + | Chr3:97734786-97734805 | MsG0380017546.01.T01:intron | 40.0% | |
| TTTGTCTGAACTTGAGTCTC+AGG | + | Chr3:97734858-97734877 | MsG0380017546.01.T01:CDS | 40.0% | |
| !! | TCAGGTTGAAAAACTGTCAC+TGG | + | Chr3:97735154-97735173 | MsG0380017546.01.T01:intron | 40.0% |
| !! | TGTAGAAGCTTTGAGAGCTA+AGG | + | Chr3:97735262-97735281 | MsG0380017546.01.T01:CDS | 40.0% |
| ATTTCCCTGCTGGACATTGA+TGG | - | Chr3:97735519-97735538 | None:intergenic | 45.0% | |
| CAAATTGCGAGTCAACCAAG+AGG | + | Chr3:97734812-97734831 | MsG0380017546.01.T01:CDS | 45.0% | |
| CAGGGAAATAGTGCCTCATA+CGG | + | Chr3:97735530-97735549 | MsG0380017546.01.T01:CDS | 45.0% | |
| GGTGAAGTTAGCTGAGGATA+TGG | + | Chr3:97735370-97735389 | MsG0380017546.01.T01:intron | 45.0% | |
| TCTTGCTGGGTTTGGAAACT+TGG | + | Chr3:97735583-97735602 | MsG0380017546.01.T01:CDS | 45.0% | |
| TTTGAATTGTGCACGCCAAC+CGG | - | Chr3:97735563-97735582 | None:intergenic | 45.0% | |
| ! | GTAGTCATGTTGAGTTGTGG+CGG | - | Chr3:97735464-97735483 | None:intergenic | 45.0% |
| ! | TTGATGGTTGGCGAAACATG+TGG | - | Chr3:97735503-97735522 | None:intergenic | 45.0% |
| ! | TTTTCTCCACCTTGACCTCT+TGG | - | Chr3:97734830-97734849 | None:intergenic | 45.0% |
| AGTCAACCAAGAGGTCAAGG+TGG | + | Chr3:97734821-97734840 | MsG0380017546.01.T01:CDS | 50.0% | |
| CCAACCATCAATGTCCAGCA+GGG | + | Chr3:97735512-97735531 | MsG0380017546.01.T01:CDS | 50.0% | |
| GGAAATAGTGCCTCATACGG+TGG | + | Chr3:97735533-97735552 | MsG0380017546.01.T01:CDS | 50.0% | |
| TGATGTGGTGACAGAGCTTC+TGG | - | Chr3:97735399-97735418 | None:intergenic | 50.0% | |
| TTGGCGAAACATGTGGCATG+CGG | - | Chr3:97735496-97735515 | None:intergenic | 50.0% | |
| ! | ATGAGGCACTATTTCCCTGC+TGG | - | Chr3:97735529-97735548 | None:intergenic | 50.0% |
| AACCGGAACACCACCGTATG+AGG | - | Chr3:97735546-97735565 | None:intergenic | 55.0% | |
| GCCAACCATCAATGTCCAGC+AGG | + | Chr3:97735511-97735530 | MsG0380017546.01.T01:CDS | 55.0% | |
| GCGAGTCAACCAAGAGGTCA+AGG | + | Chr3:97734818-97734837 | MsG0380017546.01.T01:CDS | 55.0% | |
| TCATACGGTGGTGTTCCGGT+TGG | + | Chr3:97735545-97735564 | MsG0380017546.01.T01:CDS | 55.0% | |
| TGCCTCATACGGTGGTGTTC+CGG | + | Chr3:97735541-97735560 | MsG0380017546.01.T01:CDS | 55.0% | |
| ! | CCCTGCTGGACATTGATGGT+TGG | - | Chr3:97735515-97735534 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 97734694 | 97735679 | 97734694 | ID=MsG0380017546.01;Name=MsG0380017546.01 |
| Chr3 | mRNA | 97734694 | 97735679 | 97734694 | ID=MsG0380017546.01.T01;Parent=MsG0380017546.01;Name=MsG0380017546.01.T01;_AED=0.10;_eAED=0.10;_QI=0|0|0|1|1|1|4|0|175 |
| Chr3 | exon | 97734694 | 97734710 | 97734694 | ID=MsG0380017546.01.T01:exon:13403;Parent=MsG0380017546.01.T01 |
| Chr3 | exon | 97734804 | 97734879 | 97734804 | ID=MsG0380017546.01.T01:exon:13404;Parent=MsG0380017546.01.T01 |
| Chr3 | exon | 97735158 | 97735283 | 97735158 | ID=MsG0380017546.01.T01:exon:13405;Parent=MsG0380017546.01.T01 |
| Chr3 | exon | 97735371 | 97735679 | 97735371 | ID=MsG0380017546.01.T01:exon:13406;Parent=MsG0380017546.01.T01 |
| Chr3 | CDS | 97734694 | 97734710 | 97734694 | ID=MsG0380017546.01.T01:cds;Parent=MsG0380017546.01.T01 |
| Chr3 | CDS | 97734804 | 97734879 | 97734804 | ID=MsG0380017546.01.T01:cds;Parent=MsG0380017546.01.T01 |
| Chr3 | CDS | 97735158 | 97735283 | 97735158 | ID=MsG0380017546.01.T01:cds;Parent=MsG0380017546.01.T01 |
| Chr3 | CDS | 97735371 | 97735679 | 97735371 | ID=MsG0380017546.01.T01:cds;Parent=MsG0380017546.01.T01 |
| Gene Sequence |
| Protein sequence |
