Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017860.01.T01 | XP_013462215.1 | 95.455 | 110 | 5 | 0 | 1 | 110 | 1 | 110 | 5.73E-70 | 214 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017860.01.T01 | A0A072VDK4 | 95.455 | 110 | 5 | 0 | 1 | 110 | 1 | 110 | 2.74e-70 | 214 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380017860.01 | MsG0480018522.01 | 0.818327 | 2.077139e-52 | 1.550051e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017860.01.T01 | MTR_3g113620 | 95.455 | 110 | 5 | 0 | 1 | 110 | 1 | 110 | 6.94e-74 | 214 |
| MsG0380017860.01.T01 | MTR_1g025490 | 52.294 | 109 | 45 | 1 | 1 | 102 | 1 | 109 | 5.60e-33 | 111 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017860.01.T01 | AT1G75810 | 55.200 | 125 | 38 | 2 | 1 | 110 | 4 | 125 | 2.46e-40 | 130 |
Find 36 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGAATAGGAAATATCTGTTC+TGG | 0.343522 | 3:-101575972 | None:intergenic |
| TAGAAAGCGGCGTTGGGAAT+AGG | 0.382735 | 3:-101575987 | None:intergenic |
| CGTCCGATCACCGGCTTCTC+AGG | 0.402331 | 3:+101575935 | MsG0380017860.01.T01:CDS |
| CGTGGTGGGCTTTATCCTAA+TGG | 0.420862 | 3:+101575809 | MsG0380017860.01.T01:CDS |
| ATTGTAAGGGAGGTATTTGT+TGG | 0.421438 | 3:+101575901 | MsG0380017860.01.T01:CDS |
| CCCACGTCAGCGTTGCCATT+AGG | 0.438021 | 3:-101575824 | None:intergenic |
| TCATCCACCGTTGAATTGTA+AGG | 0.438749 | 3:+101575887 | MsG0380017860.01.T01:CDS |
| CTCTGTGCTCGTACCTTCAC+CGG | 0.467220 | 3:+101575759 | MsG0380017860.01.T01:CDS |
| CTATCATTAGAAAGCGGCGT+TGG | 0.486546 | 3:-101575994 | None:intergenic |
| CGGTGTCGCCGTCCGATCAC+CGG | 0.487196 | 3:+101575926 | MsG0380017860.01.T01:CDS |
| TATCATTAGAAAGCGGCGTT+GGG | 0.494168 | 3:-101575993 | None:intergenic |
| CAGCCACGTAGATTGTTGTA+AGG | 0.502912 | 3:-101575707 | None:intergenic |
| TACAATTCAACGGTGGATGA+TGG | 0.506956 | 3:-101575884 | None:intergenic |
| CTGAAACGGACCTGAGAAGC+CGG | 0.530738 | 3:-101575945 | None:intergenic |
| TCCTAATGGCAACGCTGACG+TGG | 0.535183 | 3:+101575823 | MsG0380017860.01.T01:CDS |
| TCTGTTCTGGAATTCTGAAA+CGG | 0.536039 | 3:-101575959 | None:intergenic |
| AAAGCCGTAGAGCTTAATAC+AGG | 0.546620 | 3:-101575736 | None:intergenic |
| ATCGCCTGTATTAAGCTCTA+CGG | 0.548367 | 3:+101575732 | MsG0380017860.01.T01:CDS |
| ATGATATCAACCACCGTGGT+GGG | 0.553215 | 3:+101575795 | MsG0380017860.01.T01:CDS |
| GGACCTGAGAAGCCGGTGAT+CGG | 0.556230 | 3:-101575938 | None:intergenic |
| CTGAGAAGCCGGTGATCGGA+CGG | 0.557696 | 3:-101575934 | None:intergenic |
| AAGGGAGGTATTTGTTGGCA+CGG | 0.595376 | 3:+101575906 | MsG0380017860.01.T01:CDS |
| CATCCACCGTTGAATTGTAA+GGG | 0.599993 | 3:+101575888 | MsG0380017860.01.T01:CDS |
| CCTAATGGCAACGCTGACGT+GGG | 0.634171 | 3:+101575824 | MsG0380017860.01.T01:CDS |
| ATACCTCCCTTACAATTCAA+CGG | 0.637027 | 3:-101575894 | None:intergenic |
| ATCTCACTATCATTAGAAAG+CGG | 0.641809 | 3:-101576000 | None:intergenic |
| AATTCAACGGTGGATGATGG+TGG | 0.646287 | 3:-101575881 | None:intergenic |
| TCAACGGTGGATGATGGTGG+CGG | 0.651496 | 3:-101575878 | None:intergenic |
| CATGATATCAACCACCGTGG+TGG | 0.657627 | 3:+101575794 | MsG0380017860.01.T01:CDS |
| CCACCGTTGAATTGTAAGGG+AGG | 0.677032 | 3:+101575891 | MsG0380017860.01.T01:CDS |
| GTGGCGGTTGATAGCATACG+TGG | 0.688337 | 3:-101575862 | None:intergenic |
| CAACCTTACAACAATCTACG+TGG | 0.719583 | 3:+101575704 | MsG0380017860.01.T01:CDS |
| CATTAGGATAAAGCCCACCA+CGG | 0.727327 | 3:-101575808 | None:intergenic |
| CCTCCCTTACAATTCAACGG+TGG | 0.751522 | 3:-101575891 | None:intergenic |
| TAGGATAAAGCCCACCACGG+TGG | 0.764291 | 3:-101575805 | None:intergenic |
| TGTCATGATATCAACCACCG+TGG | 0.843191 | 3:+101575791 | MsG0380017860.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATACCTCCCTTACAATTCAA+CGG | - | Chr3:101575897-101575916 | None:intergenic | 35.0% | |
| GGAATAGGAAATATCTGTTC+TGG | - | Chr3:101575975-101575994 | None:intergenic | 35.0% | |
| TATCATGACAACAAAAACGC+CGG | - | Chr3:101575781-101575800 | None:intergenic | 35.0% | |
| !! | ATTGTAAGGGAGGTATTTGT+TGG | + | Chr3:101575901-101575920 | MsG0380017860.01.T01:CDS | 35.0% |
| !! | TCTGTTCTGGAATTCTGAAA+CGG | - | Chr3:101575962-101575981 | None:intergenic | 35.0% |
| AAAGCCGTAGAGCTTAATAC+AGG | - | Chr3:101575739-101575758 | None:intergenic | 40.0% | |
| ATCGCCTGTATTAAGCTCTA+CGG | + | Chr3:101575732-101575751 | MsG0380017860.01.T01:CDS | 40.0% | |
| CAACCTTACAACAATCTACG+TGG | + | Chr3:101575704-101575723 | MsG0380017860.01.T01:CDS | 40.0% | |
| CATCCACCGTTGAATTGTAA+GGG | + | Chr3:101575888-101575907 | MsG0380017860.01.T01:CDS | 40.0% | |
| TACAATTCAACGGTGGATGA+TGG | - | Chr3:101575887-101575906 | None:intergenic | 40.0% | |
| TCATCCACCGTTGAATTGTA+AGG | + | Chr3:101575887-101575906 | MsG0380017860.01.T01:CDS | 40.0% | |
| ATGATATCAACCACCGTGGT+GGG | + | Chr3:101575795-101575814 | MsG0380017860.01.T01:CDS | 45.0% | |
| CAGCCACGTAGATTGTTGTA+AGG | - | Chr3:101575710-101575729 | None:intergenic | 45.0% | |
| CATTAGGATAAAGCCCACCA+CGG | - | Chr3:101575811-101575830 | None:intergenic | 45.0% | |
| TGTCATGATATCAACCACCG+TGG | + | Chr3:101575791-101575810 | MsG0380017860.01.T01:CDS | 45.0% | |
| ! | AATTCAACGGTGGATGATGG+TGG | - | Chr3:101575884-101575903 | None:intergenic | 45.0% |
| !! | AAGGGAGGTATTTGTTGGCA+CGG | + | Chr3:101575906-101575925 | MsG0380017860.01.T01:CDS | 45.0% |
| CATGATATCAACCACCGTGG+TGG | + | Chr3:101575794-101575813 | MsG0380017860.01.T01:CDS | 50.0% | |
| CCACCGTTGAATTGTAAGGG+AGG | + | Chr3:101575891-101575910 | MsG0380017860.01.T01:CDS | 50.0% | |
| CCTCCCTTACAATTCAACGG+TGG | - | Chr3:101575894-101575913 | None:intergenic | 50.0% | |
| GACAACAAAAACGCCGGTGA+AGG | - | Chr3:101575775-101575794 | None:intergenic | 50.0% | |
| ! | TAGAAAGCGGCGTTGGGAAT+AGG | - | Chr3:101575990-101576009 | None:intergenic | 50.0% |
| !! | CGTGGTGGGCTTTATCCTAA+TGG | + | Chr3:101575809-101575828 | MsG0380017860.01.T01:CDS | 50.0% |
| CCTAATGGCAACGCTGACGT+GGG | + | Chr3:101575824-101575843 | MsG0380017860.01.T01:CDS | 55.0% | |
| CTCTGTGCTCGTACCTTCAC+CGG | + | Chr3:101575759-101575778 | MsG0380017860.01.T01:CDS | 55.0% | |
| CTGAAACGGACCTGAGAAGC+CGG | - | Chr3:101575948-101575967 | None:intergenic | 55.0% | |
| TAGGATAAAGCCCACCACGG+TGG | - | Chr3:101575808-101575827 | None:intergenic | 55.0% | |
| TCCTAATGGCAACGCTGACG+TGG | + | Chr3:101575823-101575842 | MsG0380017860.01.T01:CDS | 55.0% | |
| !! | GTGGCGGTTGATAGCATACG+TGG | - | Chr3:101575865-101575884 | None:intergenic | 55.0% |
| !! | TCAACGGTGGATGATGGTGG+CGG | - | Chr3:101575881-101575900 | None:intergenic | 55.0% |
| CCCACGTCAGCGTTGCCATT+AGG | - | Chr3:101575827-101575846 | None:intergenic | 60.0% | |
| CTGAGAAGCCGGTGATCGGA+CGG | - | Chr3:101575937-101575956 | None:intergenic | 60.0% | |
| GGACCTGAGAAGCCGGTGAT+CGG | - | Chr3:101575941-101575960 | None:intergenic | 60.0% | |
| !! | CGTCCGATCACCGGCTTCTC+AGG | + | Chr3:101575935-101575954 | MsG0380017860.01.T01:CDS | 65.0% |
| !! | CGGTGTCGCCGTCCGATCAC+CGG | + | Chr3:101575926-101575945 | MsG0380017860.01.T01:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 101575678 | 101576010 | 101575678 | ID=MsG0380017860.01;Name=MsG0380017860.01 |
| Chr3 | mRNA | 101575678 | 101576010 | 101575678 | ID=MsG0380017860.01.T01;Parent=MsG0380017860.01;Name=MsG0380017860.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|110 |
| Chr3 | exon | 101575678 | 101576010 | 101575678 | ID=MsG0380017860.01.T01:exon:2924;Parent=MsG0380017860.01.T01 |
| Chr3 | CDS | 101575678 | 101576010 | 101575678 | ID=MsG0380017860.01.T01:cds;Parent=MsG0380017860.01.T01 |
| Gene Sequence |
| Protein sequence |
